共查询到18条相似文献，搜索用时 250 毫秒
1.
为探索长穗颈基因促进水稻上部节间伸长的生理机制,以分别携有长穗颈基因euil、eui2和野生型基因E以的水稻协青早不育系和保持系为材料,测定了在不同节间伸长期不同器官内源GA、ABA和IAA的含量变化。结果表明：在最上节间剧烈伸长前,携有eui基因的水稻内源GA含量在不同的器官里都剧烈增加。内源ABA含量在节间里也表现相同的趋势,携有eui基因水稻的GA与ABA含量的比值显著高于E以基因的水稻．携有eui2基因水稻的内源激素含量及GA与ABA含量的比值介于euil和Eui基因之间。可见eui基因主要是通过在不同器官里产生大量的GA来促进上部节间的显著伸长,其中euil基因在促进上部节间伸长方面强于eui2。而IAA促进上部节间伸长方面作用不显著。 相似文献
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水稻窄叶突变体nal7(t)的遗传分析与基因定位 总被引：1，自引：1，他引：0
本研究以恢复系缙恢10号为试验材料,经过EMS诱变,对水稻叶突变进行了研究。结果表明,群体中发现一个窄叶突变体,表现为叶片变窄、节间变细、结实率降低等一系列突变表型。成熟期的功能叶片宽度为野生型的74.69%,倒一、二、三节的宽度分别为野生型的45.10%、57.38%、74.63%,总叶脉数为野生型的67.36%。遗传分析表明该突变性状受一对隐性核基因控制,利用SSR标记将其定位在第3染色体长臂RM14379和RM14427之间,遗传距离分别为2.1cM和3.0cM。因与nal7位于相同的染色体区段,暂命名为nal7(t)。 相似文献
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水稻长节间基因对GA3敏感性和不育系改良 总被引：25，自引：0，他引：25
带有长节间基因eui水稻不论在苗期和抽穗期对GA3的敏感性均高于等位的Eui基因，尤其对茎部的第1和第2节间影响较大，属1-2节间伸长型。带有Eui基因则对第2和第3节间伸长影响较大，属2-3节间伸长型。带有eui基因的珍长保持系作为供体，将eui导入野败不育系二九南1号、二九青、V20以及矮败不育系协青早，使其穗伸出长度提高了82 相似文献
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水稻黄绿叶基因YGL4的遗传分析和分子定位 总被引：10，自引：3，他引：7
通过EMS诱变恢复系缙恢10号,获得了一个稳定遗传的全生育期黄绿化叶色突变体。其叶绿素总含量稳定在2.01~2.28 mg g-1之间,仅有对照的38.2%~50.5%。与对照相比,黄绿叶突变体的有效穗和株高显著下降,而主穗长、一次枝梗数、主穗实粒数、结实率、千粒重则无明显差异。遗传分析表明该性状受一对隐性核基因控制,命名为YGL4。利用微卫星标记将YGL4定位于第10染色体微卫星标记RM3123和RM590之间,分别距其7.6 cM和7.8 cM。在两标记间进一步设计SSR引物,将该黄绿叶基因定位于RM1162和RM7093之间,分别距其1.8 cM和4.0 cM。为该YGL4基因的分子标记辅助选择育种和图位克隆奠定了基础。 相似文献
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eui基因作为"杂交稻种子生产的第四遗传要素",二十多年来,备受国内外遗传育种学者的关注.最近,关于水稻穗颈伸长基因EUI的定位与克隆、功能鉴定及eui基因在水稻育种中的应用取得了较大的进展.本文概述了长穗颈基因eui的发现、定位与克隆、作用机制及其在水稻育种中的应用. 相似文献
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水稻抗白叶枯病新基因Xa32（t）的鉴定和初步定位 总被引：1，自引：0，他引：1
通过多菌系接种鉴定及抗谱分析,并与目前国际上已知抗白叶枯病基因比较,证明在水稻抗源C4064中含有一个新的抗白叶枯病基因,暂命名为Xa32(t)。应用分离集团分析法(BSA),借助SSR和EST等分子标记,对该基因进行了分子标记定位。通过对F2分离群体及F3家系单株进行遗传连锁性检测,发现6个位于水稻第11染色体长臂末端的分子标记RM27256、RM27274、RM2064、ZCK24、RM6293和RM5926与Xa32(t)基因连锁。它们与Xa32(t)基因间的遗传距离分别为2.1、1.0、1.0、0.5、1.5和2.6 cM。其中标记RM6293和RM5926位于染色体近端粒一侧,其他4个标记RM27256、RM27274、RM2064和ZCK24位于基因的另一侧。将Xa32(t)定位在水稻第11染色体长臂末端2.0 cM范围内。 相似文献
9.
水稻抗白叶枯病新基因Xα32(t)的鉴定和初步定位 总被引：1，自引：0，他引：1
通过多菌系接种鉴定及抗谱分析,并与目前国际上已知抗白叶枯病基因比较,证明在水稻抗源C4064中含有一个新的抗白叶枯病基因,暂命名为Xα32(t).应用分离集团分析法(BSA),借助SSR和EST等分子标记,对该基因进行了分子标记定位.通过对F2分离群体及F3家系单株进行遗传连锁性检测,发现6个位于水稻第11染色体长臂末端的分子标记RM27256、RM27274、RM2064、ZCK24、RM6293和RM5926与Xα32(t)基因连锁.它们与Xα32(t)基因间的遗传距离分别为2.1、1.0、1.0、O.5、1.5和2.6 cM.其中标记RM6293和RM5926位于染色体近端粒一侧,其他4个标记RM27256、RM27274、RM2064和ZCK24位于基因的另一侧.将Xα32(t)定位在水稻第11染色体长臂末端2.0 cM范围内. 相似文献
10.
水稻抗白叶枯病新基因Xa32（t）的鉴定和初步定位 总被引：2，自引：0，他引：2
通过多菌系接种鉴定及抗谱分析,并与目前国际上已知抗白叶枯病基因比较,证明在水稻抗源C4064中含有一个新的抗白叶枯病基因,暂命名为Xa32(t)。应用分离集团分析法(BSA),借助SSR和EST等分子标记,对该基因进行了分子标记定位。通过对F2分离群体及F3家系单株进行遗传连锁性检测,发现6个位于水稻第11染色体长臂末端的分子标记RM27256、RM27274、RM2064、ZCK24、RM6293和RM5926与Xa32(t)基因连锁。它们与Xa32(t)基因间的遗传距离分别为2.1、1.0、1.0、0.5、1.5和2.6 cM。其中标记RM6293和RM5926位于染色体近端粒一侧,其他4个标记RM27256、RM27274、RM2064和ZCK24位于基因的另一侧。将Xa32(t)定位在水稻第11染色体长臂末端2.0 cM范围内。 相似文献
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The elongation and development of rice uppermost internode plays an important role in plant architecture development. In general, the sheathed panicle phenomenon in rice sterility line is caused by the elongation and development hindrance of the uppermost internode. The study on molecular mechanisms underlying sheathed panicle would be helpful for improving plant architecture in sterility line. At the present study, we reported the study on a sheathed panicle mutant, named sui2, originated from a tissue-culture progeny. Its uppermost internode severely shortened, resulted in its pancleen closed by flag leaf sheath, without significantly length alternation at other internodes. The cytological analysis demonstrated that the shorten of the uppermost internode is caused by insufficient elongation of the parenchyma cells. Genetic analysis of the progeny derived from the cross-combination of sui2 and IRAT129 revealed that sui2 is a single gene dominant mutant. Linkage analysis to 608 normal individuals from F2 generation showed that SUI2 was located in a 110 kb region delimited by InDel marks S4-14.1 and S4-14.2 on the end of chromosome 4 long arm. The annotated genes on this region did not display any difference in genomic sequence, while the expression level of LOC_Os04g39430, encoding a cytochrome P450 protein and an allele of D11, increased by 264 times expression amount in mutant. Analysis of qRT-PCR for several crucial genes on BR (brasssinolide) signaling pathway showed that in mutant the expression level of all these genes increased, indicating that genes in BR signaling pathway may be involved in the regulation to the elongation and development of uppermost internode. 相似文献
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Panicle enclosure is a typical phenotype of almost all male-sterile rice lines. An elongated uppermost internode (eui) mutant exhibited notably rapid elongation of the uppermost internode at the heading stage; this is considered as a potential
mechanism to eliminate panicle enclosure. We developed thermo-sensitive genic male-sterile (TGMS) eui mutants that were characterized by notably elongated uppermost internodes. The elongation of the uppermost internode in the
TGMS eui mutant Changxuan 3S (CX) is mainly attributed to an increase in cell number and cell elongation, the latter being the more
significant process. Temperature treatments revealed that the effects of temperature on panicle exsertion were similar to
those on fertility and that the most temperature-sensitive stage coincides with the period from the formation of the pollen
mother cell to meiosis during panicle initiation. These results indicate that elongation increases as temperature decreases
and that the expression of the eui gene is more efficient at low temperatures than at high temperatures. In hybrid rice seed production using the TGMS eui mutant, the temperature range should be optimized at 24–28°C in order to preserve the development of completely male-sterile
pollen and to eliminate panicle enclosure. Consequently, by using TGMS eui rice lines, gibberellin application can be avoided, thereby reducing the cost of hybrid seed production.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
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利用EMS诱变籼型水稻恢复系缙恢10号,获得一个稳定遗传的矮化脆性突变体dbc1,苗期即表现矮化、叶片变脆,一直保持到成熟。与原始亲本相比,突变体的各节间均显著缩短,株高仅58.93 cm,略有包穗,属于dn型矮化变异,对赤霉素的敏感性显著下降,有效穗、千粒重和结实率无明显变化,穗长、穗粒数和实粒数则极显著下降。进一步分析发现,dbc1的茎秆和叶片的载荷强度极显著下降,纤维素含量无变化,木质素含量则略有下降,差异达显著水平。遗传分析表明该性状受1对隐性核基因调控,利用886株西农1A/dbc1的F2变异单株,最终把DBC1基因定位在第2染色体SSR标记RM13943和RM13952之间,物理距离仅197 kb,含有52个注释基因。这为下一步调控基因的克隆和dbc1材料的育种应用奠定了基础。 相似文献
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矮秆突变体是小麦育种和株高遗传研究的重要基因资源。通过‘云麦53’成熟种子的EMS (Ethyl methyl sulfonate)诱变及诱变植株连续自交,获得了33个M3代候选突变体。通过诱变亲本与M2和M3代候选植株的株高差异分析,筛选到26个矮秆突变体,其株高变幅为(13.61±0.11)~(44.08±1.73) cm。基于8个矮秆基因的12个特异性标记检测发现, 26个矮秆突变体至少携带2个矮秆基因标记位点。除株高外, 26个矮秆突变体还携带穗长、小穗密度、节间数和平均节间长4个不同突变性状。26个矮秆突变体可聚为5个亚类,第1亚类的小穗数和小花数最少;第2亚类的株高最矮,穗长和平均节间长最短,小穗密度最高;第3亚类突变体的节间数最少。株高与平均节间长和节间数呈极显著相关,偏相关系数分别为0.94、0.58,但与穗长、小穗数、小花数和小穗密度4个性状无相关性。26个矮秆突变体的株高与平均节间长和节间数关联遗传,携带不同的突变基因组合,可用于小麦矮化育种,以及株高、穗长和小穗密度等性状的遗传机制研究。 相似文献
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Genetic dissection of plant height by molecular markers using a population of recombinant inbred lines in maize 总被引：3，自引：0，他引：3
Tang Ji-hua Teng Wen-tao Yan Jian-bing Ma Xi-qing Meng Yi-jiang Dai Jin-rui Li Jian-Sheng 《Euphytica》2007,155(1-2):117-124
Plant height is an important trait for maize breeding because it is related to planting density and lodging resistance. It
is influenced by many qualitative genes and quantitative trait loci (QTL). In this study, the genetic basis of plant height
and its related traits were dissected, using simple sequence repeat (SSR) markers with a maize population of 294 recombinant
inbred lines (RIL). Correlation results showed that plant height had a significant positive correlation with leaf number,
average internode length and internode number. Increased plant height was affected most by average internode length. Six QTL
for plant height were detected, which were consistent with those reported in previous studies. Moreover, eight QTL for leaf
number, seven for internode number and six for average internode length were identified. Four of six QTL detected for average
internode length were located on the same chromosomal region as the QTL affecting plant height and shared common molecular
markers. This latter result strongly suggests that average internode length was the main contributor to plant height in maize. 相似文献
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Ma Yu Shuang-Lin Mao Guo-Yue Chen Zhi-En Pu Yu-Ming Wei You-Liang Zheng 《Euphytica》2014,200(1):95-108
Spike length (SL) is one of the most important components of spike morphology, and the uppermost internode represents an ideal organ to study the transportation system. We performed conditional and unconditional quantitative trait locus (QTL) mapping in two unrelated recombinant inbred line populations to precisely detect QTLs for uppermost internode length (UIL) and SL, and to dissect the genetic relationship between these two factors with plant height (PH). Both of the populations were derived from crosses with synthetic wheat. Ten repetitive QTLs for UIL and six environment-independent QTLs for SL were identified in this study, and twelve of these were completely independent of PH. Conditional QTL mapping analysis indicated that SL was more independent to PH than UIL was. The results indicated that the conditional QTL mapping method could evaluate PH component effects on PH, and thus accelerate the selection of suitable loci that improve commercial wheat morphology yet avoid changes to PH. 相似文献