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1.
Woo-Sung Jung Kyu-Jong Lee Byun-Woo Lee 《Journal of Crop Science and Biotechnology》2015,18(4):209-218
High temperature-induced spikelet sterility is expected to become a major factor reducing rice yield under the future climate conditions. To examine the responses of spikelet sterility to air temperature, humidity, and temperatures of panicle and spikelet, two japonica rice cultivars in different maturity groups were exposed to four sets of different temperature conditions from initial heading stage in 2013 and 2014. The temperature conditions included ambient temperature (AT), AT + 1.5°C, AT + 3.0°C, and AT + 5.0°C. Spikelet fertility showed a wide range of variation from 100 to 4.6% depending on temperature treatments. The ridge regression revealed that not only air temperature but also vapor pressure deficit (VPD) was negatively associated with spikelet fertility. The spikelet fertility was well fitted to logistic equations not only of air temperature, spikelet internal temperature, and panicle surface temperature but also of VPD. No clear difference in the accuracy of sterility estimation was observed for models that use air temperature, panicle or spikelet temperature as inputs. In contrast, the logistic equation model that uses both air temperature and VPD as independent variables had better accuracy in predicting spikelet sterility. These results suggested that spikelet or panicle temperature would be no better predictor for high temperature-induced spikelet sterility than air temperature. Therefore, further study is merited to verify the VPD effects on spikelet sterility under high temperature conditions. 相似文献
2.
High temperature‐induced grain sterility in rice is becoming a serious problem in tropical rice‐growing ecosystems. We studied the mechanism of high temperature‐induced grain sterility of different rice (Oryza sativa L) cultivars at two relative humidity (RH) levels. Four varieties of Indica and Japonica rice were exposed to over 85 % RH and 60 % RH at 36/30 °C, 34/30 °C, 32/24 °C and 30/24 °C day/night air temperatures from late booting to maturity inside sunlit phytotrons. Increasing both air temperature and RH significantly increased spikelet sterility while high temperature‐induced sterility decreased significantly with decreasing RH. Neither Indica nor Japonica rice types were superior to the other in the response of their spikelets to increased air temperature and RH. Increased spikelet sterility was due to increased pollen grain sterility which reduced deposition of viable pollen grains on stigma. Reduction in sterility with decreased RH was more due to decreased spikelet temperature than to air temperature. Thus the impact of RH should be considered when interpreting the effect of high temperature on grain sterility. Spikelet fertility was curvilinearly related to spikelet temperature. Grain sterility increased when spikelet temperature increased over 30 °C while it became completely sterile at 36 °C. The ability of a variety to decrease its spikelet temperature with decreasing RH could be considered as avoidance while the variability in spikelet sterility among varieties at a given spikelet temperature could be considered as true tolerance. 相似文献
3.
Kyu-Jong Lee Da-Ik Kim Kwang Soo Kim Byun-Woo Lee 《Journal of Crop Science and Biotechnology》2014,17(2):53-57
This experiment was conducted to evaluate the varietal differences of spikelet sterility response to air temperature during the reproductive stage. Six rice varieties differing in maturity group (early-maturing; Unkwangbyeo, Odaebyeo, medium-maturing; Andabyeo, Hwasungbyeo, and mid-late maturing; Donganbyeo, Chuchungbyeo) were grown under ambient temperature (AT) conditions before being transferred to the temperature-controlled plastic houses. For the synchronization of the growth stage, 15 rice seedlings (2011) and 10 rice seedlings (2012) per pot were transplanted in a circle and only main stems were grown by removing tillers at early stage of their emergence. At the initial heading stage, pots for each variety were transferred to the four plastic houses that were controlled to AT, AT + 1.5°C, AT + 3.0°C, and AT + 5.0°C, respectively. Spikelet fertility was significantly decreased due to high temperature-induced spikelet sterility at AT + 3.0 and/or AT + 5.0°C treatment during flowering time in 2011. Spikelet fertility in 2012 was much lower than in 2011 even at the AT treatment because of high temperature-induced spikelet sterility at the micosporogenesis stage. Critical temperature (Tc) that induces 50% spikelet sterility at flowering time was estimated by fitting the temperature response of spikelet fertility to a logistic function. Tc ranged from 34.6°C (Odaebyeo) to 39.7°C (Hwasungbyeo), Odaebyeo being significantly more sensitive to high temperature-induced spikelet sterility than the other varieties. This result has shown that response of spikelet sterility to higher temperature is different according to rice varieties. However, further study should be done to arrive at a concrete conclusion. 相似文献
4.
A genetic analysis of low-temperature-sensitive sterility in indica-japonica rice hybrids 总被引：9，自引：0，他引：9
Low-temperature-sensitive sterility has become one of the major obstacles in indica–japonica hybrid rice breeding. The objectives of this paper were to evaluate the extent of the fertility reduction and to determine the genetic basis of low-temperature-sensitive sterility. Seventeen varieties were crossed in various ways to produce 21 F1s including 16 indica-japonica hybrids. Fertility of the F1s and their parents was examined under both high and low temperature conditions. Considerable reduction in spikelet fertility was observed under low-temperature conditions in the majority of the indica–japonica hybrids having at least one wide compatibility parent. However, the extent of fertility reduction varied greatly, depending on the parental genotypes. Data from five pairs of reciprocal crosses indicated that the cytoplasm had no effect on fertility reduction. The more-or-less bimodal distribution of the fertility segregation of one BC1F1 and two F2: populations under low-temperature conditions suggested that the low-temperature-sensitive sterility was controlled by only one or a few genes. It was also shown that the low-temperature-sensitivity is not related to wide compatibility. We conclude that it is possible to develop indica-japonica hybrids with wide compatibility and also insensitivity to the low-temperature conditions. 相似文献
5.
A breeding program was initiated at the International Rice Research Institute (IRRI) in 1990 to develop thermosensitive genic
male sterile (TGMS) rice lines for developing two-line rice hybrids for the tropics. The TGMS trait was transferred from a
temperate japonica TGMS mutant, Norin PL 12, to indica and tropical japonica rice varieties using the pedigree selection procedure.
Six new TGMS rice lines adapted to tropical conditions were developed which showed complete pollen and spikelet sterility
when maximum temperature was higher than 30 °C 1–2 week after panicle initiation. However, up to 85.5% spikelet fertility was observed when these lines were exposed to
26–29 °C during the critical stage. Using two of these TGMS lines, some heterotic rice hybrids showing 1–1.6 t/ha higher grain yield
than the inbred check varieties were identified in unreplicated observational yield trial conducted at IRRI. Two of the six
two-line hybrids yielded significantly higher than the check variety in a replicated preliminary yield trial.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
6.
Genetic divergence among cold tolerant rices (Oryza sativa L.) 总被引：1，自引：0，他引：1
Summary Genetic divergence was investigated among 17 rice varieties known to possess some degree of cold tolerance at different growth stages. The 17 varieties and their 102 F1 hybrids with 6 male testers were studied for isozyme variation at 15 loci, spikelet fertility, and degree of cold tolerance at various stages. Multivariate analyses of the data provided several schemes of divergence based on various sources of evidence. All schemes gave similar results, and separated the varieties into japonica and indica groups. The japonica group displayed specific isozymes, a low F1 fertility with indica testers, and a high degree of cold tolerance which was expressed in the F1 progenies. The indica group displayed contrasting specific isozymes, a high F1 fertility with indica testers, and a moderate to low degree of cold tolerance which was not expressed in the F1 progenies. One variety, ARC 6000, displayed unique traits in most schemes and was classified into a distinct type based on the isozymes. The results emphasize that cold tolerance is a major trait for classification of rice into two varietal groups and that proper characterization of potential donors is essential in breeding. Isozyme studies are useful tools for this purpose. 相似文献
7.
一个新的水稻花粉半不育性位点的定位分析 总被引：1，自引：0，他引：1
利用一套以籼稻珍汕97B为背景的粳稻日本晴染色体片段代换系,鉴定发现1个半不育的代换系。全基因组基因型分析表明,该代换系仅含3个粳稻导入片段,而其他遗传背景与珍汕97B相同。在湖北武汉和海南分别种植其衍生的F2和F3分离群体,采用单标记分析和区间作图法分析花粉育性和小穗育性的数量性状位点(QTL),结果表明,该代换系的半不育性是第2染色体上的粳稻导入片段引起的,该片段RM262~RM475区间存在1个新的影响花粉育性的QTL,其贡献率为13.9%。研究结果将为进一步精细定位水稻育性QTL以及鉴定相关功能基因提供重要的试验基础。 相似文献
8.
利用一套以粳稻品种Asominori为遗传背景、籼稻品种IR24为染色体片段供体的覆盖全基因组的CSSL群体，研究了籼粳亚种间组合Asominori/IR24和02428/IR24杂种小穗低育性的遗传基础。结果发现，Asominori/IR24组合的育性主要受第5染色体上的2个育性位点S-24(t)和S-31(t)及第6染色体上的 S-5位点控制，其中S-31(t)为本研究发现的新育性位点，粳稻品种02428带有该位点的亲和性基因。02428/IR24组合的低育性主要受S-24(t)花粉育性位点的影响。育性基因的表达受遗传背景的影响，在粳稻遗传背景中，S-24(t)位点处在Si/Sj杂合基因型时可使杂种小穗育性下降70%左右，而S-31(t)和 S-5为杂种半不育位点。在籼粳全基因组杂合遗传背景中，当S-5i/S-5j基因型置换成S-5i/S-5i基因型后，亚种间杂种小穗育性可平均提高22.5%，接近正常育性水平。在S-5i/S-5j遗传背景中，S-24(t)和S-31(t)的Si/Si纯合基因型不能改善亚种间杂种的小穗育性。说明S-5位点是影响亚种间小穗育性的关键位点，在亚种间杂交稻育种中，必须首先克服S-5位点造成的育性障碍。提出了等位基因置换法克服水稻籼粳亚种间杂种小穗低育性的技术策略。 相似文献
9.
Norin‐PL8 (‘PL8’) is an extremely cool‐tolerant line of rice in Japan that contains genes for cool tolerance originating from a javanica landrace. It was investigated to see whether the dwarfing gene d18‐k (kotaketamanishiki dwarf) exerts its pleiotropic effect on enhancing the cool tolerance at the booting stage in the genetic background of PL8. The d18‐k isogenic line of the recurrent parent PL8 (D8), PL8, and two commercial cultivars ‘Hayayuki’ and ‘Kirara 397’ were used. For each line/cultivar, the 12°C‐5‐day treatment was conducted at various times during the booting stage. In addition to spikelet fertility, the ratio (%) of the fertilized‐spikelet number of each treated panicle to the varietal mean of fertilized‐spikelet number per panicle in the control (FS‐T/C) was adopted to estimate cool temperature damage. For FS‐T/C, the lines‐cultivars ranked in the order of D8 > PL8 > ‘Hayayuki’ > ‘Kirara 397’, reflecting their cool tolerances. D8 exceeded PL8 in both pollen grain number per anther in the control and as an indicator of pollen fertility after the treatment, as a result of the effects of d18‐k. Consequently, d18‐k can be used to develop super‐highly cool‐tolerant cultivars for cool‐weather areas. 相似文献
10.
Genetics, fertility behaviour and molecular marker analysis of a new TGMS line, TS6, in rice 总被引：1，自引：0，他引：1
The thermosensitive genic male sterility (TGMS) system has great potential for revolutionizing hybrid rice production through simple, less expensive and more efficient seed production technology. For the successful utilization of this novel male sterility system, knowledge of the breeding and fertility behaviour of a TGMS line is essential. In this study, the fertility transformation behaviour, the critical fertility and sterility temperatures and the mode of inheritance of male sterility were studied for a new TGMS line, TS6, identified at Tamil Nadu Agricultural University, Coimbatore, India. The pollen and spikelet fertilities recorded on plants raised at fortnightly intervals revealed that this line was completely sterile for 78 consecutive days (35/22 to 32/23°C, maximum/minimum temperatures) and reverted to fertile when the temperature was 30/18°C. It remained fertile continuously for 69 days and the maximum pollen and spikelet fertilities recorded were 75 and 70%, respectively. The fertility was highly influenced by daily maximum temperature followed by average and minimum temperatures. It was not influenced by relative humidity, sunshine hours or photoperiod. The critical temperature inducing sterility and fertility was 26.7 and 25.5°C, respectively. The male sterility in TS6 was inherited as a monogenic recessive in the F2 and BC1 populations of TS6 × MRST9 as well as TS6 × IR68281B. Using bulked segregant analysis on an F2 population of TS6 × MRST9, an RAPD marker, OPC052962, was identified to be associated with TGMS in TS6. 相似文献
11.
Extreme temperatures cause spikelet sterility in rice and thus yield losses. Predicting sterility is difficult because organ temperature may differ from air temperature. Four rice genotypes were planted under irrigated flooded conditions in a similar replicated design in four environments: the relatively humid dry season in the Philippines, the summer season in southern France and the cold-dry and hot-dry seasons in northern Senegal. Panicle temperature was measured by IR photography on ca. 4000 images, complemented with simultaneous monitoring of micro-climatic variables on the floodwater-canopy-air continuum. Spikelet sterility was observed at the population scale at grain maturity, and canopy morphology was also characterized (plant height, leaf area index, panicle position within the canopy and panicle exertion). The period and time of day of anthesis (TOA) was estimated using a model developed on the same experiments as described in a previous paper. Panicle temperature varied between 9.5 °C below and 2 °C above air temperature at 2 m. During TOA it was on average slightly warmer than the air in the Philippines and significantly colder in Senegal. Spikelet sterility was disaggregated into three components caused by chilling at microspore stage, incomplete panicle exertion at anthesis and high panicle temperature at anthesis. Chilling caused up to 100% and heat up to 40% sterility, the former mainly in the Senegal cool-dry season and the latter in the Philippines. All genotypes avoided heat sterility in the hot-dry season in Senegal despite air temperatures up to 40 °C, by a combination of escape (early TOA) and avoidance (transpiration cooling). Only one genotype had no chilling induced sterility due to physiological tolerance. It is concluded that heat stress causing sterility is more likely to occur in warm-humid than hot-arid environments due to humidity effects on transpiration cooling. Models predicting global warming effects on sterility losses need to consider microclimate and organ temperature, and research is now needed on the genetic control of panicle transpiration cooling 相似文献
12.
The African rice Oryza glaberrima, traditionally cultivated since more than 3.500 years, is of poor agronomic performance but resistant/tolerant to various
stresses and diseases. The introduction of these characters into O. sativa cultivars is difficult since crossing barriers cause spikelet sterility in F1. Backcrossing can restore fertility and recently
facilitated the development of fertile O. glaberrima × O. sativa ssp. japonica hybrid progenies for rain fed systems. With the objective to gain access to African rice germplasm for improvement of irrigated
rice, crosses were performed with eighteen O. glaberrima and twenty O. sativa ssp. indica accessions. In total about one hundred F1-hybrid grains were obtained. The F1 plants were all completely sterile and backcrossing
(BC) to O. sativa was performed in order to restore spikelet fertility. Monitoring of Tog5681 × IR64 hybrid progenies under field conditions
revealed a broad genetic diversity within the BC1 and BC2 populations. Some BC1 and BC2 progeny plants outperformed the O. sativa parent, indicating that the heterozygocity level and complementary gene action after two backcrosses are still sufficient
to positively influence plant vigor. Spikelet fertility of progenies was highly variable, but almost complete fertility was
already observed within the BC1F2 population. High spikelet fertility was preserved in one out of two analyzed BC1F3 families and inmost of the BC2F3 families. The ability to restore spikelet fertility within few generations and the potential of the genetic diversity present
in interspecific progenies facilitates the development of plant types specifically designed for the African irrigated and
lowland environment.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
13.
Moderate to high heterosis for yield and ten related characters was studied in 45 crosses involving six indica (I) and four
tropical japonica (J) varieties of rice under three (E1-optimum sowing and high fertility, E2-both optimum sowing and fertility and E3- late sowing and high fertility) environments. Trends of magnitude of heterosis for grain yield and plant height were I/J
> I/I > J/J and for days to 50% flowering I/J > J/J > I/I hybrids. Estimates of standard heterosis (%) for grain yield were
–64.5 to 146.1 in E1, –70.4 to 82.2 in E2 and –67.2 to 63.8 in E3. Environment E1 seemed to be more favorable for higher heterosis expression than others. Higher heterosis in yield also accompanied heterosis
in panicle number, dry matter and spikelet and grain number per panicle. Most estimates for days to flower were negative.
Heterotic I/J hybrids also recorded maximum heterosis for earliness. Moderate to low standard heterosis for plant height across
environments (2.0 to 13.7%) was recorded. Hybrids were identified in specific environments for direct exploitation
in hybrid breeding. Hybrids B4116 / Sarjoo 52, B4122 / Pant Dhan 4 and B4122 / Narendera 359 were more stable than others
over three environments.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
14.
15.
开花期低温胁迫对四川攀西稻区水稻开花结实的影响 总被引：7，自引：0，他引：7
以3种籼稻品种和3种粳稻品种为试验材料, 利用人工气候室在开花期进行低温胁迫处理, 研究了低温胁迫对攀西稻区籼、粳稻开花和结实及两者间关系的影响。结果表明, 开花期低温胁迫下籼稻和粳稻的开花习性、花药和花粉特性和结实表现不同。常规粳稻开花对低温有较高的耐冷性。低温胁迫下籼稻品种(组合)花药体积、花药开裂率、可育花粉率、柱头着花粉数和柱头花粉萌发率较对照降低幅度均比粳稻品种(组合)大; 籼稻品种(组合)各产量构成因素较对照降低幅度比粳稻品种(组合)大。相关分析表明, 结实与水稻花药和花粉主要性状有密切关系。开花期低温胁迫影响花药和花粉发育成熟, 使花药不能正常开裂、散粉不足, 可育花粉率和柱头花粉萌发率降低, 直接影响受精结实, 成为结实率降低的主要原因之一。 相似文献
16.
An analysis of hybrid sterility in rice using a diallel cross of 21 parents involving indica, japonica and wide compatibility varieties 总被引：3，自引：0，他引：3
Summary There is much interest in the inter-varietal fertility of Asian cultivated rice. In this study, we analyzed the fertility of hybrids in a diallel set of 210 crosses involving 21 parents representing a broad range of the cultivated rice germplasm including landraces, primitive cultivars, modern elite cultivars and parents of hybrid rice. The materials were also carefully selected with respect to indica-japonica classification including typical indica/japonica, more or less intermediate and wide compatibility varieties. The level of hybrid fertility varied widely among the crosses from almost completely sterile to fully fertile. In general, hybrid fertility of intra-subspecific crosses, i.e., indica by indica (I×I) and japonica by japonica (J×J), is much higher than inter-subspecific crosses (I×J or J×I). The fertility varied widely in hybrids involving wide compatibility varieties and also in inter-subspecific crosses not involving wide compatibility varieties. An analysis of variance showed that both the main effects of indica and japonica parents and the interaction between the parents are highly significant in determining hybrid fertility. We speculate that, in addition to wide compatibility, the overall genetic difference between the indica and japonica parents, that may involve a series of minor mutations, play an important role in determining the hybrid fertility. There are also genes of sizable effect that influence fertility in hybrids resulting from specific combinations of the parents.Abbreviations I×I
indica by indica cross
- I×J
indica by japonica cross
- J×I
japonica by indica cross
- J×J
japonica by japonica cross
- WCV
wide compatibility variety 相似文献
17.
Expression of thermosensitive genic male sterility in rice under varying temperature situations 总被引：1，自引：0，他引：1
Response of thermosensitive genic male sterility in rice to varying temperature situations was studied by using four TGMS
lines. In three sets of maximum, minimum and their combined temperatures, it was observed that maximum temperature played
a predominant role in influencing sterility/fertility of TGMS lines under the combined regimes. Expression of a TGMS gene
was found to be influenced by the genetic background of the recipient lines. Exposure for more than 8 hours of 32 °C was found necessary to induce complete male sterility in indica TGMS lines: IR68945-4-33-4-14 and IR68949-11-5-31 while,
more than 4 hours of such exposure was enough to induce sterility in case of their japonica donor Norin PL 12. Sudden interruption
with 27 °C even for 2 hours under the sterility inducing regime of 32/24 °C could induce partial fertility in the line IR68945-4-33-4-14. However, the line ID 24 remained completely sterile even with
10 hours of interruption with 27 °C. An interruption with lower temperature of 27 °C for 4 hours for even one day induced partial fertility in IR68945-4-33-4-14. The period of four to eight days after panicle
initiation was the stage most sensitive to temperature variations. Hybrid rice breeders need to develop numerous genetically
diverse TGMS lines, which possess critical sterility inducing temperature of 28 °C and are not affected by sudden interruptions with a lower temperature for few hours daily for a couple of days.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
18.
Presence of substantial heterosis and economic hybrid seed production are two most desirable components for success of any commercial hybrid breeding programme. Thermosensitive genic male sterile (TGMS) lines of rice, in this regard, have tremendous potential in realizing further quantum jump in yield and economical hybrid seed cost. Analyses for combining ability and heterosis over optimum (120N : 60P2O5 : 40K2O kg/ha) and high (200N : 90P2O5 : 60K2O kg/ha) fertility environments for six traits were made in 2 years (2001 and 2002) using 120 hybrids of inter‐ and intra‐subspecific nature derived from hybridization of 30 elite indica TGMS lines and four cultivars, viz., ‘Pant Dhan 4’ and ‘Ajaya’ (I = indica), ‘Taichung 65’ (J = japonica) and ‘IR 65598‐112‐2’ (TJ = tropical japonica) in line × tester mating design. Predominance of non‐additive genetic variance suggested good prospects of hybrid breeding. Pooled analysis revealed highly significant variances for lines, general combining ability (GCA), specific combining ability (SCA) and line x tester. TGMS line 365‐8S was the best general combiner for all the six traits including grain yield. Trend of relative mid‐parent heterosis for grain yield, panicle length, grain number per panicle and earliness in flowering was I/TJ > I/J > I/I. For panicle number per plant and 1000‐grain weight, trends were I/TJ > I/I > I/J and I/I > I/TJ > I/J, respectively. Grain yield recorded heterosis of 49.3%, 71.9% and 92.7% for I/I, I/J and I/TJ hybrid groups respectively. Effect of environments on the hybrid performance indicated better response of hybrids at high fertilizer dose. Study suggests greater prospects of combining improved japonica and tropical japonica germplasms having wide compatible gene with indica TGMS lines for exploitation of intersubspecific heterosis. 相似文献
19.
Wide‐compatibility varieties are a special class of rice germplasm that is able to produce fertile hybrids when crossed to either indica or japonica subspecies. Previous studies determined the f5 allele from ‘Dular’ (f5‐Du), f6 allele from ‘Dular’ (f6‐Du) and S5 allele from ‘02428’ (S5‐08) as neutral alleles conferring wide‐compatibility. However, the possible extent of the effect of the three neutral alleles has not been fully characterized because of the narrow range of the tester varieties used and the highly complex differentiation in Asian cultivated rice. In this study, we further developed the five near‐isogenic lines with higher recovery rates of the recurrent parent genome, and testcrossed to 14 japonica varieties, which have been widely used in rice breeding programmes in China. The results clearly revealed that all three neutral alleles exhibited substantial effects on spikelet fertility in most of the indica–japonica testcrosses, which indicated that these hybrid sterility loci have been extensively differentiated between indica and japonica varieties. The magnitudes of effects on spikelet fertility averaged over various crosses seem to be similar among the three neutral alleles, with f5‐Du, f6‐Du and S5‐08 alleles increasing spikelet fertility by 15.09%, 13.99% and 14.25%, respectively. The testcrosses involving f5‐Du allele generally showed much smaller variation in pollen fertility than others. The pyramiding lines with two neutral alleles showed a wider spectrum and a higher level of wide compatibility than others, whereas most of the increases in hybrid fertility couldn’t be simply explained by additive effects, suggesting the very complexity of wide compatibility and hybrid sterility. The indica–japonica hybrids involving restorer lines as one of their parents showed much higher pollen fertility (almost normal) and also higher spikelet fertility. The implications of the findings in rice breeding programmes are also discussed. 相似文献
20.
Hybrids between indica and japonica rice varieties usually show partial sterility, and are a major limiting factor in the
utilization of heterosis at subspecific level. When studying male-gamete (pollen) abortion, a possibly important cause for
sterility, six loci (S-a, S-b, S-c, S-d, S-e and S-f) for F1 pollen sterility were identified. Here we report genetic and linkage analysis of S-c locus using molecular markers in a cross between Taichung 65, a japonica variety carrying allele S-c
j, and its isogenic line TISL5, carrying alleleS-c
j. Our results show that pollen sterility occurring in the hybrids is controlled by one locus. We used 208 RFLP markers, as
well as 500 RAPD primers, to survey the polymorphism between Taichung 65 and TISL5. Six RFLP markers located on a small region
of chromosome 3, detected different RFLP patterns. Co-segregation analysis of fertility and RFLP patterns with 123 F2 plants confirmed that the markers RG227, RG391, R1420 were completely linked with the S-c locus. The genetic distances between the markers C730, RG166 and RG369 and the S-c locus were 0.5 cM, 3.4 cM, and 3.4 cM respectively. Distorted F2 ratios were also observed for these 4 RFLP markers in the cross. This result suggests that the `one locus sporo-gametophytic'
model could explain F1 hybrid pollen sterility in cultivated rice. RG227, the completely linked marker, has been converted to STS marker for marker-assisted
selection.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献