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设置N施肥量为18 kg/667 m2,P2O5施肥量为3~12 kg/667 m2,K2O施肥量为14~26 kg/667 m2,研究不同磷、钾肥施用量对贵州遵义辣椒产量的影响。研究结果表明,辣椒N、P2O5、K2O最佳施肥量为18、6~12、18~22 kg/667 m2时,干辣椒产量可达250 kg/667 m2以上。 相似文献
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通过大田试验,研究了控释肥在土壤中的养分释放规律,及其对当年生平邑甜茶实生苗生长发育的影响。结果表明:控释肥在土壤中养分的释放速率分为增加、高峰、减小3个阶段;控释肥对养分持续、稳定的供应满足了平邑甜茶在整个生长期内对养分的需求,促进了作物的生长。在施肥量相等的情况下,控释肥CRF1处理(N、P2O5、K2O施用量分别为630、150、480kg/hm2)作物的各项生长指标都显著高于速效肥SF处理;即使施肥量降低的控释肥CRF2(N、P2O5、K2O施用量分别为504、120、384kg/hm2)、CRF3(N、P2O5、K2O施用量分别为320、120、400kg/hm2)处理,其作物各项生长指标都不低于SF处理。 相似文献
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以"中荷10号"黄瓜为试材,采用"3414"试验设计方案,对冬棚黄瓜的NPK肥配施效应进行了分析。结果表明:随着NPK肥施用量的增加,黄瓜产量均呈先增后减趋势,符合肥料报酬递减律,增产效应为NP2O5K2O;NP、NK具有正向互作效应,而PK为反向互作效应,最高产量的N∶P2O5∶K2O配合比率为424.32∶251.01∶219.03,最高产量达57 038.23kg/hm2,最佳种植效益的配合比率为388.16∶248.25∶159.15,可获得最佳种植效益128 215.27元/hm2;全肥区产量较高,与高N区、高P区、高K区无显著差异,且产投比相对较高,是最优处理组合。 相似文献
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平衡施肥对宁夏银川日光温室番茄产量的影响 总被引：3，自引：0，他引：3
试验采用四元二次回归通用旋转组合设计,在测土分析的基础上,研究了有机肥、氮肥、磷肥、钾肥对宁夏银川郊县二代节能日光温室番茄产量的影响效应,建立了产量与有机肥、氮肥、磷肥、钾肥4因子的肥料试验模型,并采用降雏法对所建立的数学模型进行分析,确定其最佳施肥量.试验结果表明,供试土壤供肥特点为低氮、高磷、高钾,且因以往施肥量大而排水不畅,土壤表现为轻度盐渍化特征;番茄的产量对有机肥、氮肥、磷肥、钾肥作用的反应为氮肥>钾肥>有机肥>磷肥;番茄达到理论最高产量122.97 t/hm2时,4种肥料的施肥量组合方案为:有机肥(M)7 500 kg/hm2,N 867.6 kg/hm2,P2O5 60 kg/hm2,K2O 405 kg/hm2;而番茄产量稳定达到115.26 t/hm2以上,且有利于土壤可持续利用,4种肥料的适宜施用量为:有机肥15 000 kg/hm2,N 850 kg/hm2,P2O5 300 kg/hm2,K2O 400 kg/hm2,N:P2O5:K2O的比例为8.5:3:4. 相似文献
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本文通过对N、P、K的3因素、4水平、3次重复试验研究,结果表明:马铃薯80%的产量来源于土壤的供肥能力,而N、P、K的合理配方,增产幅度可达20%～40%。在低肥力的土壤上,N、P、K最佳施肥方案为每667m2纯N:2.8～6.1kg、P2O5:2.6～3.5kg、氧化钾:0.3～3.8kg。 相似文献
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AIM: To observe the inhibitory effect of madecassoside on the LPS-stimulated microglia and to investigate its possible mechanism. METHODS: Microglia cells of neonatal Sprague-Dawley (SD) rats were cultured, isolated and purified. Microglia cells were activated with lipopolysaccharide (LPS). The inhibitory effect of madecassoside on microglia was measured by MTT assay. Tumor necrosis factor alpha (TNF-α), interleukin 1β (IL-1β) were detected by ELISA. Cell cycle and apoptotic rate were evaluated by flow cytometry. The expression of TLR4 was detected by Western blotting. The expression of NF-κB was detected by RT-PCR. RESULTS: LPS induced the proliferation of microglia and release inflammatory cytokines significantly. Compared with LPS group, madecassoside inhibited the proliferation of microglia induced by LPS in a dose dependent manner. The IC50 value of madecassoside was 10.97 nmol/L to microglia after incubation for 48 h. Madecassoside also decreased the levels of TNF-α and IL-6, increased the ratios of microglia at the G2 phase and the apoptotic rate, decreased the expression of TLR4 and NF-κB significantly (P<0.05). CONCLUSION: Madecassoside has inhibitory effects on the proliferation of LPS-stimulated microglia, by which the mechanism may be related to inhibition of the expression of TLR4 and NF-κB, change of cell cycle distribution and induction of microglia apoptosis. 相似文献
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AIM: To investigate the effects of fucoidan on the angiogenesis of multiple myeloma cells in vitro, and its related mechanisms. METHODS: The human multiple myeloma RPMI 8226 cells and human endothelial cells were cultured in vitro. The growth inhibition rate of RPMI 8226 cells was examined by MTT assay. The cell cycle and apoptosis rate were measured by flow cytometry. RPMI 8226 cells were treated with fucoidan for 72 h, and the cell culture supernatant was collected. The VEGF concentration was examined by ELISA, and the tube formation assay was applied to assess the angiogenic activity. After treatment with fucoidan for 72 h at different concentrations, the protein levels of HIF-1α, VEGF, p-AKT and p-ERK1/2 were detected by Western blot. RESULTS: Fucoidan inhibited the growth of RPMI 8226 cells in a dose- and time-dependent manner. After treatment with fucoidan for 72 h, the cell cycle was arrested at G1 phase, and the apoptotic rate of RPMI 8226 cells was increased with the increasing concentration of fucoidan, which was much higher than that in control group (P<0.05). The VEGF concentration was significantly decreased with the increa-sing concentration of fucoidan. The numbers and areas of the capillary-like structures decreased while the concentration of fucoidan increased, and those at 100 mg/L were less than those in the control (P<0.05). The protein levels of HIF-1α, VEGF, p-AKT and p-ERK1/2 in fucoidan group were significantly lower than those in control group (P<0.05). CONCLUSION: Fucoidan inhibits the secretion of VEGF in multiple myeloma cells, and reduces angiogenesis induced by multiple myeloma cells. It inhibits the protein expression of HIF-1α and VEGF, which may be related to inhibiting the phosphorylation of AKT and ERK1/2. 相似文献
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CHANG Jing-zhi WANG Chen LI Yi-chuan LIU Qing SHEN Yong-jie LU Kun GUO Ya-li ZHANG Shan-feng WANG Ming-chen 《园艺学报》2018,34(5):930-933
AIM:To investigate the effect of lutein on the viability of breast cancer cells and its possible mechanism. METHODS:The human breast cancer T47D cells were divided into control group and lutein (6.25, 12.5, 25, 50 mg/L) treatment groups. The effect of lutein on the viability of T47D cells was measured by MTT assay. The mRNA expression of nuclear factor erythroid 2-related factor 2 (Nrf2), glutathione peroxidase 1 (GPx1) and superoxide dismutase 2(SOD2) was detected by RT-qPCR. Fluorescent probes DCFH-DA was used to determine the production of reactive oxygen species (ROS). The protein expression of Nrf2 and p65 was determined by Western blot. RESULTS:The MTT results showed that lutein inhibited T47D breast cancer cell viability in a dose-and time-dependent manner. The RT-qPCR results showed that the mRNA levels of Nrf2, GPx1 and SOD2 were higher in lutein treatment groups than those in the control group (P<0.05), and with the increased concentrations and extension of intervention time of lutein, the relative mRNA levels were all increased. The ROS levels were significantly decreased in the lutein-treated groups (P<0.05). The results of Western blot demonstrated that the protein expression of Nrf2 was significantly increased (P<0.05), and p65 protein was decreased (P<0.05) in a dose-dependent manner with lutein treatment for 48 h. CONCLUSION:Lutein significantly inhibits the viability of breast cancer cells, and the inhibition roles may be related to up-regulation of the expression of Nrf2, antioxidant enzymes GPx1 and SOD2 mRNA expression and down-regulation of oxidative stress, thus blocking the NF-κB signaling pathway. 相似文献
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AIM: To investigate the effect of toosendanin (TSN) on invasion and migration abilities of human ovarian cancer cells and the related mechanism. METHODS: The human ovarian cancer cell lines CAVO-3 and SKVO-3 were treated with TSN at different concentrations. The cell viabilty at 12, 24, 48, 72 and 96 h after TSN treatment was measured by CCK-8 assay. Scratch wound healing assay and Transwell assay were employed to measure the invasion and migration abilities of CAVO-3 cells. The protein expression of nuclear factor-κB (NF-κB) p65, E-cadherin, N-cadherin, vimentin and Snail was determined by Western blot. RESULTS: TSN significantly inhibited the viability of CAVO-3 and SKVO-3 cells (P<0.05). Compared with control group, the migration and invasion abilities of CAVO-3 cells in TSN group decreased significantly (P<0.05). In addition, the expression of NF-κB p65 and E-cadherin protein increased notably, followed with N-cadherin, vimentin and Snail protein decreased significantly (P<0.05). However, the inhibitor of NF-κB BAY11-7082 reversed the impact above. Compared with TSN group, the migration and invasion abilities in TSN+BAY11-7082 group increased significantly (P<0.05). The protein expression of E-cadherin also decreased notably, followed with the protein expression of N-cadherin, vimentin and Snail increased significantly (P<0.05). CONCLUSION: TSN inhibits the invasion and migration abilities of human ovarian cancer cells, which is related to the inhibition of epithelial-mesenchymal transition process mediated by NF-κB/Snail signaling pathway. 相似文献
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AIM:To study the effect of dexamethasone(Dex) on the expression of CD18. METHODS:Quantitative RT-PCR analysis, Northern blotting technique were used to measure the expression of CD18 in U937 cells treated by PMA.RESULTS:Dex could significantly attenuated the effects of PMA in a dose-dependent manner (10-6 mol/L-10-10 mol/L). These effects of Dex (10-7 mol/L) were completely aborted by RU-486 (10-6 mol/L).CONCLUSION:Dex, via GR, could inhibit CD18 mRNA expression in U937 cells treated by PMA. The effects of Dex might be possibly depended on the counteracting action on the NF-κB. 相似文献
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Neurons in the mammalian central nervous sysytem (CNS) are highly sensitive to the availability of oxygen. Hypoxia alters synaptic transmission in a few minutes. Both glutamatergic and γ-aminobutyric acid (GABA)ergic synaptic transmissions respond to hypoxic exposure with prominent modification. Glutamate receptors, GABA receptors, adenosine receptor, and some endogenous neuromodulators are involved in the preservation of neuron function. Since the neuroprotection in all hypoxic tolerant species examined so far relies on significant increase in GABA and decrease in glutamate, it may be an important strategy to make a moderate balance of glutamate/GABA synaptic transmission against hypoxic insults. 相似文献
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AIM: To investigate the combined effect of octreotide and Dachaihu decoction on the treatment of severe acute pancreatitis(SAP). METHODS: Wistar rats(n=50) were randomly divided into sham group, SAP group, octreotide group, Dachaihu decoction group and combination group. The quantity of ascites was measured. The levels of amylase, alanine aminotransferase and creatinine in the serum were examined. The morphological changes of the pancreatic tissues were observed by HE staining. The activation of NF-κB and IκBα expression were determined by Western blot. The mRNA expression with ICAM-1 and IL-1 was detected by qPCR.RESULTS: Combined treatment with octreotide and Dachaihu decoction effectively reduced the quantity of ascites and the levels of amylase, alanine aminotransferase and creatinine in the serum in SAP rats. Moreover, combined treatment significantly inhibited SAP-induced activation of NF-κB and decrease in IκBα protein expression, accompanied by a decrease in ICAM-1 and IL-1 mRNA expression. CONCLUSION: Combination of octreotide with Dachaihu decoction effectively attenuates SAP by inhibiting NF-κB signaling pathway and ICAM-1 and IL-1 expression. 相似文献
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AIM: To explore the effect of genistein on ammonia-induced nuclear factor-κB (NF-κB) activation and the underlying mechanism.METHODS: Primary astrocyte cultures were prepared and challenged with NH4Cl to establish a hyperammonemic model. The activation of ERK, Akt and NF-κB was examined by Western blot.RESULTS: AG1478 and genistein significantly inhibited ammonia-induced activation of ERK and Akt. Ammonia-induced NF-κB nuclear translocation was significantly inhibited by the pretreatment of LY294002, genistein and AG1478.CONCLUSION: Genistein significantly inhibited ammonia-induced ERK activation and Akt-mediated NF-κB activation, which might represent the important mechanism by which this naturally occurring substance exerts its swelling-inhibiting effect. 相似文献
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AIM: To investigate whether L-carnitine (LC) treatment confers renoprotection in a rat model of streptozotocin (STZ)-induced diabetic nephropathy (DN). METHODS: Diabetic animal model was established by intraperitoneal injection of STZ (65 mg/kg) in Sprague-Dawley rats. Diabetic rats were treated with LC (50 mg·kg-1·d-1 or 200 mg·kg-1·d-1 intravenously) daily for 12 weeks. The effects of LC on STZ-induced DN were evaluated by assessing renal function, urinary protein excretion, histopathological changes, macrophage infiltration, the expression of proinflammatory and prosclerotic cytokines, and the expression of nuclear factor-κB (NF-κB) and apoptosis-related gene. RESULTS: LC administration significantly decreased glomerulosclerosis, preserved the number of podocytes, and reduced macrophage infiltration. These changes were accompanied by improvements in urinary protein excretion and renal dysfunction. LC treatment suppressed the expression of proinflammatory and prosclerotic cytokines, and these changes were paralleled by significant attenuation of NF-κB and apoptosis-related gene expression. CONCLUSION: LC has a renoprotective effect against STZ-induced DN in rats. 相似文献
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AIM: To investigate the effects of simvastatin on the expression of Toll-like receptor 2 (TLR-2), interferon-γ (IFN-γ) and monocyte chemoattractant protein-1 (MCP-1) in lung tissues of mice with mouse cytomegalovirus (MCMV) pneumonia and to explore the possible mechanism. METHODS: Male BALB/c mice (6~8 weeks old, n=40) were randomly divided into 5 groups: normal control (NC) group, MCMV infection group, simvastatin group 1 (SMV1 group), simvastatin group 2 (SMV2 group), and simvastatin group 3 (SMV3 group). The mice in SMV1, SMV2 and SMV3 groups were gavaged with simvastatin (50 mg·kg-1·d-1 for 7 d) 7 d before, on the same day of and 3 d after intraperitoneal injection of MCMV, while the mice in normal control group and MCMV infection group were gavaged with the same volume of normal saline. HE staining was used to observe the pathological changes of lung tissues in mice. Total tissue protein was extracted from the lung homogenates to detect the expression of TLR-2 by Western blot and immunohistochemical staining. Real-time PCR was used to analyse the content of MCMV DNA. The levels of IFN-γ and MCP-1 were measured by enzyme-linked immunosorbent assay (ELISA). RESULTS: Compared with NC group, the pathological changes of the lung tissues of the mice in MCMV group showed alveolar interstitial edema, alveolar wall widening and a large number of inflammatory cells. The expression of TLR-2 in the lung tissues of the mice in model group was increased significantly. The content of MCMV DNA was increased, and the expression of IFN-γ and MCP-1 was also increased significantly. Compared with the mice in MCMV group, the pathological changes of the lung tissues of simvastatin groups showed that the inflammatory cells were decreased. The expression of TLR-2 was down-regulated. The content of MCMV DNA was decreased, and the levels of IFN-γ and MCP-1 were also decreased significantly. At the same time, the expression of TLR-2 and the content of MCMV DNA in SMV1 group were less than those in SMV2 and SMV3 groups (P<0.05), and no statistically significant difference between SMV2 and SMV3 groups was observed. CONCLUSION: Simvastatin down-regulates the TLR-2 signaling pathway, and reduces the expression of TLR-2 and replication of MCMV DNA, thus attenuating the pathological damage of the lung tissue. Early intervention with simvastatin plays an important role in preventing the infection of MCMV and reducing the inflammation. 相似文献