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干旱、高盐和低温等非生物胁迫严重影响植物的生长发育和作物产量。转录因子在调节植物生长发育以及对外界环境胁迫的响应方面起着重要作用。DREB转录因子含有一个保守的AP2/EREBP结构域,参与外界环境胁迫的应答响应,通过结合DRE(Dehydration responsive element)顺式作用元件,调控下游胁迫相关基因的转录表达,改良植物的抗性。本文在前人研究的基础上,综述了DREB转录因子的结构特征、介导的信号传递途径、对非生物胁迫的响应以及转基因的研究进展,旨在为作物的抗逆育种提供理论依据。  相似文献   

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赵黎明 《北方水稻》2009,39(6):63-69
随着我国科技力量的飞速发展,科学家们在激素受体基因分离鉴定、激素控制株型以及激素间的相互作用等方面取得了突破性进展,其中生长素(IAA)在协调体内外调节机制中起着不可或缺的作用;细胞分裂素(CTK)可以通过对细胞分裂与分化的调节而参与对水稻生长发育的调控;赤霉素作为植物生长的必需激素之一,调控植物生长发育的各个方面,如:种子萌发,下胚轴的伸长,叶片的生长和水稻开花时间等;脱落酸(ABA)同样也是一种重要的植物激素,受到生物胁迫和非生物胁迫的调控,在水稻对胁迫耐受性和抗性中发挥着重要作用;乙烯(ETH)则是一种多功能的植物激素,它在水稻生长发育和应对生物及非生物胁迫过程中起着重要作用。本文着重阐述了近几年植物激素在生理学研究方面的研究进展,提出了我国在植物激素研究领域的未来发展方向与趋势,同时也展望了植物激素研究对水稻重要农业性状改良的意义。  相似文献   

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植物非生物胁迫应答的分子机制   总被引:4,自引:0,他引:4  
非生物胁迫因子是制约植物生长发育、影响作物产量和质量的关键因子。这些非生物胁迫的共同点是它们都会导致植物细胞缺水,使细胞的水分平衡紊乱,还可以引起蛋白质等大分子变性,破坏植物细胞内的膜结构等。为了生存,植物在遇到非生物胁迫时不得不在形态和生理生化代谢上进行一些调整,以适应或忍耐环境胁迫。揭示植物胁迫应答分子机理是人们长期以来探索的重大课题。非生物胁迫引起的应答非常复杂并且常常相互关联,干旱、高盐、低温等胁迫可以引起相似的应答反映,如积累大量的渗透调节剂、重建细胞内离子动态平衡、修复被破坏的膜系统、清除活性氧自由基等等。近年来,胁迫应答的分子机理研究成果颇丰,结合笔者等的研究,本文简要进行了综述。  相似文献   

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Plants have evolved delicate mechanisms to cope with environmental stress. Following exposure to environmental stimuli, extracellular signals are perceived and transmitted through signal transduction cascades. Upon receipt and transmission of the signals, a number of stress-related genes are induced, leading to stress adaptation in plant cells. Rice, which is a critical food grain for a large portion of the world’s population, is frequently impacted by several abiotic stressors, the most important of which are drought, salinity, and cold. Exposure to environmental conditions outside of acceptable tolerance ranges can negatively affect rice growth and production. In this paper, a review of rice responses to abiotic stress is presented, with particular attention to the genes and pathways related to environmental stress tolerance. It is apparent that, while progress has been made in identifying genes involved in stress adaptation, many questions remain. Understanding the mechanisms of stress response in rice is important for all research designed to develop new rice varieties with improved tolerance.  相似文献   

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Setsuko Komatsu 《Rice》2008,1(2):154-165
Proteomics techniques have identified a vast number of proteins that participate in the growth of plants or their adaptation to environmental stresses. Functional analysis of those proteins will contribute to the development of high-yielding crops through artificial manipulation of the basic life phenomena of plants or through the assessment of their stress tolerance. The conditions in almost all farmlands are suboptimal for plant growth, resulting in a calculation that causes the loss to three quarters of the potential yield of crops. Breeding of stress-tolerant cultivars has been hampered by the paucity of information on molecular events underlying stress tolerance. A comparative analysis of the response of plants to stress at the protein level, together with physiological measurements, will assist in identifying the genes and pathways that are crucial for stress tolerance. This review examines analyses of the rice proteome under abiotic stress and the potential contribution of proteomics technology in the creation of abiotic stress-tolerant plants.  相似文献   

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《Field Crops Research》2006,97(1):66-76
Low and unstable rice productivity in many areas of Asia is associated with many abiotic and biotic stresses such as drought, salinity, anaerobic conditions during germination, submergence, phosphorus and zinc deficiency, etc. To develop rice varieties with tolerance to these stresses, we undertook a large backcross (BC) breeding effort for the last 6 years, using three recurrent elite rice lines and 203 diverse donors, which represent a significant portion of the genetic diversity in the primary gene pool of rice. Significant progress has been made in the BC breeding program, which resulted in development of large numbers of introgression lines with improved tolerance to these stresses. Promising lines have been developed with excellent tolerances (extreme phenotypes) to salinity, submergence and zinc deficiency; resistance to brown plant hopper, ability to germinate under the anaerobic condition and low temperature. Our results indicated that there exist tremendous amounts of ‘hidden’ diversity for abiotic and biotic stress tolerances in the primary gene pool of rice. Furthermore, we demonstrated that despite the complex genetics and diverse physiological mechanisms underlying the abiotic stress tolerances, introgression of genes from a diverse source of donors into elite genetic backgrounds through BC breeding and efficient selection (careful screening under severe stress) is a powerful way to exploit this hidden diversity for improving abiotic stress tolerances of rice. We have developed three large sets of introgression lines, which not only provide an unique platform of breeding materials for developing new rice cultivars with superior yield and stability by trait/gene pyramiding, but also represent unique genetic stocks for a large-scale discovery of genes/alleles underlying the abiotic and biotic stress tolerances of rice using genomic tools.  相似文献   

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水稻经常遭遇干旱、高盐、低温、病原菌等逆境胁迫,影响其生长发育甚至产量。NAC转录因子是植物特有的、最大的转录因子家族之一,在水稻生长发育及抵御多种非生物及生物胁迫反应中具有重要的调控作用。本文阐述了水稻NAC转录因子的结构、分类及染色体定位,并阐述了NAC转录因子在水稻抗旱、耐盐、耐冷及抗病等抗逆基因工程中应用的研究进展,为NAC转录因子的利用及水稻抗逆遗传改良提供参考。  相似文献   

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Salinity-alkalinity is incipient abiotic stress that impairs plant growth and development. Rice (Oryza sativa) is a major food crop greatly affected by soil salinity and alkalinity, requiring tolerant varieties in the saline-alkali prone areas. Understanding the molecular and physiological mechanisms of saline-alkali tolerance paves the base for improving saline-alkali tolerance in rice and leads to progress in breeding. This review illustrated the physiological consequences, and molecular mechanisms especially signaling and function of regulating genes for saline-alkali tolerance in rice plants. We also discussed QTLs regarding saline-alkali tolerance accordingly and ways of deployment for improvement. More efforts are needed to identify and utilize the identified QTLs for saline-alkali tolerance in rice.  相似文献   

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花生生育期易遭受干旱、低温、高盐等非生物胁迫,影响其出苗、开花、营养物质积累等过程,从而造成花生产量和品质的下降。在作物遭受非生物胁迫时,通过转录因子调控下游功能基因的表达,是植物应对胁迫的一 种重要调控模式。本文对非生物胁迫相关的转录因子NAC、AP2/ERF、bZIP、MYB等基因家族的结构、功能及相关基因在花生抗逆反应中的研究进展进行了综述,为花生抗逆分子育种提供参考。  相似文献   

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大麦耐盐性鉴定方法及耐盐机理研究进展   总被引:3,自引:0,他引:3  
盐分胁迫是导致作物减产的主要非生物因素之一,大麦是公认的耐盐性较强的作物之一,其耐盐机理复杂。本文简要评述了近年来国内外鉴定大麦耐盐性的主要方法,并对大麦耐盐机理进展做了综述。  相似文献   

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Soybean (Glycine max) is one of the most important economic legume crops with largest planting area, and is also an important oil crop, as well as food and feed material. Soybean-rhizobia symbiosis plays important roles in plant cultivation and fertilizer application. However, there are many problems in agricultural application of soybean symbiotic nitrogen fixation. In this review, we summarized three restriction factors (host specificity, low nitrogen fixation efficiency and abiotic stress) and discussed research progresses of these factors. Clarification of host specific mechanism will help to select and apply rhizobia inoculants. Both maintaining high nitrogenase activity and delaying nodule senescence can improve the efficiency of symbiotic nitrogen fixation. Abiotic stress-tolerant rhizobia can improve the abiotic stress tolerance of soybean. Breeding stress tolerant genotypes of soybean and rhizobia, obtaining correlated genes are the common strategies to improve soybean symbiotic nitrogen fixation under extreme conditions. Regulatory mechanisms of these restriction factors are still poorly understood and needs further clarification.  相似文献   

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WOX转录因子在植物的生长发育和非生物胁迫响应中起着重要的调控作用。文章基于全基因组数据,从茶树基因组中鉴定出29个WOX基因,并对其基因结构、进化关系、保守域、染色体定位进行分析,同时分析了它们在PEG诱导的干旱胁迫、盐胁迫处理中的转录组数据。结果表明,29个CsWOX(茶树WOX)基因在茶树染色体上分布不均;根据进化关系将茶树WOX基因分为4类;基因结构和保守基序分析发现相同亚家族的基因结构和保守结构域基本一致;基因表达分析显示,CsWOX基因在花和果实组织中具有较高的表达水平,且部分基因随着叶片成熟度的增加,表达水平升高;不同的Cs?WOX基因在PEG诱导的干旱胁迫、盐胁迫处理下存在差异表达,说明CsWOX基因广泛参与茶树生长发育并在响应非生物胁迫中发挥重要作用。该结果将为进一步研究WOX基因在调控茶树生长发育和非生物胁迫响应中的作用提供一些有价值的信息,为茶树WOX基因的功能研究与利用提供科学依据。  相似文献   

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Drought is one of the major abiotic stresses which adversely affect crop plants limiting growth and yield potential.Structural and functional characterization of drought stress-induced genes has contributed to a better understanding of how plants respond and adapt to the drought stress.In the present study,differential display technique was employed to study the gene expression of rice plants at the reproductive stage that were subjected to drought stress by withholding water,Pseudomonas fluorescens strain(Pf1) treated plants subjected for drought stress by withholding water and control(well-watered).Differentially expressed c DNAs of six genes(COX1,PKDP,b ZIP1,AP2-EREBP,Hsp20 and COC1) were identified,cloned and sequenced.Real-time q PCR analysis showed that all the six genes were upregulated in drought-stressed plants treated with Pf1.This revealed that the remarkable influence of Pf1 colonization leads to drought tolerance at the reproductive stage.These results showed that high levels of gene expression in plants lacking adequate water can be remarkably influenced by Pf1 colonization,which might be a key element for induced systemic tolerance by microbes.  相似文献   

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Abiotic stress confers serious damage to the photosynthetic machinery, often resulting in plant growth inhibition. Hypothetical chloroplast open reading frame 3 (Ycf3)-interacting protein 1 (Y3IP1) is a nucleus-encoded thylakoid protein and plays an essential role in the assembly of photosystem I. The full-length cDNA over-expresser (FOX) gene-hunting system is an approach using systemically generated gain-of-function mutants. Among the FOX-rice lines, a line CE175 overexpressing rice Y3IP1 gene (OsY3IP1) displayed less inhibition of root growth under saline (NaCl) stress. The expression of OsY3IP1 was up-regulated under saline and alkaline (Na2CO3) stresses in the rice variety Kitaake. After saline and alkaline treatments, transgenic Kitaake overexpressing OsY3IP1-GFP (OsY3IP1-GFPox/Kit) displayed higher levels of chlorophyll content compared to Kitaake. Under the stress conditions, the maximum quantum yield of photosystem II photochemistry levels was higher in OsY3IP1-GFPox/Kit than in Kitaake. The increased tolerance conferred by OsY3IP1 overexpression correlated with reduced reactive oxygen species accumulation. Our data provide new insights into the possible role of OsY3IP1 in the pathway suppressing photooxidative damage under stress conditions. These features can be further exploited to improve saline and alkaline tolerances of rice plants in future.  相似文献   

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Drought stress is one of the major environmental factors affecting crop growth and productivity. Cuticular wax plays essential roles in protecting plants from environmental stress via forming a hydrophobic barrier on leaf epidermis. In this study, we analyzed nine members (OsCUT1?OsCUT9) of β-ketoacyl-CoA synthase, the rate-limiting key enzyme for cuticular wax synthesis in rice by homology search and domain prediction. The expression levels of OsCUT genes under different abiotic stresses were investigated and OsCUT1 down-regulated by abiotic stress was selected for further function validation. Compared to the wild type, overexpression of OsCUT1 (OX-OsCUT1) exhibited significantly increased drought resistance. Epicuticular wax was increased on the leaf surface of OX-OsCUT1 and the chlorophyll leaching experiment showed that the cuticular permeability was decreased in the OX-OsCUT1 plants. Moreover, overexpression of OsCUT1 didn’t result in the significant changes of major agronomic traits. In total, these results suggested that OsCUT1 is a promising gene for engineering rice plants with enhanced drought tolerance.  相似文献   

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