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1.
Summary Clones derived from Beta vulgaris and Beta maritima were assayed for their ability to detect restriction fragment length polymorphisms (RFLPs) in different beet accessions. The clones able to detect polymorphism were used as genetic markers to assess the degree of genetic variation existing between and within different species of the genus Beta. The data support the current taxonomy of the Beta vulgaris section, while the great genetic similarity found between Beta webbiana and Beta procumbens indicates that they could belong to the same species.Enough variation was found between Beta vulgaris cultivars, allowing the isolation of a sufficient number of genetic markers for the construction of detailed genetic maps.  相似文献   

2.
Summary Six Vicia faba populations were grown in all possible combinations with six Rhizobium leguminosarum strains in order to estimate the relative importance of the three genetic components of symbiotic variability. Additive genetic effects of host genotype and rhizobium genotype accounted for only 8.9% and 11.8% of the total phenotypic variation. Non-additive variation attriabutable to specific host genotype × rhizobium genotype interactions was by far the largest component of variation and accounted for 73.8% of phenotypic differences. Therefore the greatest improvement in symbiotic nitrogen fixation is likely to arise from simultaneous selection of both symbionts.  相似文献   

3.
Wild relatives of crop plants offer an attractive gene pool for cultivar improvement. We evaluated genetic and phenotypic variation for a set of 72 Israeli accessions of wild barley from 21 populations. These populations were grouped further into four ecotypes. In addition, environmental variables describing the local conditions for the populations were used to infer the environmental divergence. Genetic, phenotypic and environmental distances were estimated from the data and UPGMA dendrograms constructed. The results showed that genetic variation was larger between populations than within them, whereas for phenotypic measurements variation was larger within populations than between them. No significant correlation was found between genetic and phenotypic similarities, or between phenotypic and environmental similarities, whereas a weak correlation between genetic and environmental similarities was detected. Twenty-three AFLP-markers were identified to be ecotype specific. Chromosomal location was known for five of these markers. Four of the five ecotype specific markers were correlated with both phenotypic traits and environmental variables.  相似文献   

4.
Summary Random amplified polymorphic DNA (RAPD) analysis was carried out to evaluate polymorphism and genetic similarity between 39 Mediterranean mandarin genotypes. One hundred eleven amplification products were identified using 21 random primers. An average of 2.2 RAPD markers was obtained for each primer, corresponding to 42% of the amplification products. Genotype-specific RAPD markers were also found, mainly in known hybrids. UPGMA cluster analysis revealed the low level of genetic variation between accessions of Mediterranean mandarins, whereas their hybrids with other Citrus species showed greater genetic dissimilarity. Twenty accessions yielded very similar patterns, suggesting either that they could be a single clone, or that the technique was not able to detect genomic variation. However, for the other specimens genetic polymorphism can easily be detected by RAPD, although the genetic variation between accessions was quite low. The large number of hybrids and the low polymorphism between accessions support the hypothesis that Mediterranean mandarins are all true hybrid of Common mandarins (Citrus reticulata Blanco).  相似文献   

5.
Seven wild populations of Anemonecoronaria were assessed for 11 phenotypic traits, most of them having economic value for the flower industry. The wild populations were sampled to represent the diversity in habitats, climates, rock and soil types, terrains, and elevations in Israel. AFLP analysis was carried out on 12 individuals from each of six out of the seven wild populations and for six individuals from the commercial cultivar ‘Mona-Lisa’. It was found that the Dorot population, which is located in the area bordering the semi-arid zone at the very end of the species distribution, exhibits extreme and different phenotypes with relatively low variability compared withthe other wild populations. The other six wild populations, that grow in more favorable geographic and climatic conditions exhibit phenotypes with larger plants, larger numbers of flowers and less dissected leaves. These populations were less uniform than that of Dorot. Genetic characterization by AFLP markers revealed a total of 165 bands. The wild populations exhibit wide variation within-population, with about 80% polymorphic bands and average gene diversity between pairs of about 30%. The Dorot population has the lowest genetic variation and the Megido population the highest. Thus, the phenotypic variation reflects the genetic variation. The cultivar ‘Mona-Lisa’, as expected, has much lower genetic variation. The Dorot population and the ‘Mona-Lisa’ cultivar were found to have the largest genetic distances from the other wild populations, and the highest genetic variation between themselves. Phenetic analysis yielded a dendrogram describing the genetic relatedness of these populations. This revised version was published online in August 2006 with corrections to the Cover Date.  相似文献   

6.
Summary All current commercial cultivars of leeks are open-pollinated and one of the major problems with the crop is poor uniformity with much of the variation being genetic in origin. Inbred lines and single cross hybrids were produced to try to reduce the genetic variation. Inbreds were generated by single seed descent from 5400 plants taken from six commercial cultivars and performance data for uniformity, yield and quality are presented for the S1-S3 generations. A few relatively vigorous inbred lines were obtained but overall, inbreeding depression was very severe with no compensating increase in uniformity as measured by coefficients of variation. In contrast, the experimental hybrids gave significant uniformity, yield and quality benefits compared to open-pollinated commercial cultivars and can be used as the basis for developing a range of commercial hybrid cultivars.Abbreviations SSD Single Seed Descent - HRI Horticulture Research International  相似文献   

7.
Summary Anthers of the diploid (2n=77) and the colchi-tetraploid (2n=154) Pelargonium roseum were cultured in vitro. In both ploidy level anthers containing uninucleate or binucleate microspores were incubated on a modified White's medium. Calli formed were then subcultured on Murashige and Skoog's medium for organoid differentiation. Plants developed from organoids were transferred to filter paper bridges and after that transplanted into pots. Plants derived from anthers of the tetraploid had diploid chromosome number. Wide variation of their essential oil components suggested their genetic heterogeneity. Further, high correlations between different seasons in the rate of essential oil components showed that the wide variation was due to genetic differences. Therefore, these plants probably originated from pollen grains. On the other hand, plants derived from anthers of the diploid had diploid chromosome number. Small variation and low correlations between different seasons in essential oil components indicated their genetic homogeneity. Their origin was ascribable to the somatic tissues of the mother plant. It is concluded that in plant species in which usual sexual reproduction is difficult, anther culture of chromosome-doubled plants will give a useful method for obtaining genetic variation.  相似文献   

8.
Genetic diversity of wheat wild relatives in the Near East detected by AFLP   总被引：3，自引：0，他引：3  
In order to reveal the molecular genetic diversity of wheat wild relatives, an AFLP analysis was conducted with 16 accessions of five Triticum andAegilops species originating from the Near East. Variation within population was studied with at least seven individuals per accession. Four primer combinations were used for selective amplification. Based on the scored bands, we estimated percentage of polymorphic bands, 1 – proportion of shared bands (1-psb) and nucleotide diversity (π). Of the five species used in this study, Ae. speltoides had the highest level of `within population' variation. This species had also the highest value of the variation among populations. As for Triticum species, the level of variation within population was low in diploid species (T. urartu and T. boeoticum),whereas two tetraploid species (T. dicoccoides and T. araraticum) had relatively high levels of variation within population. While the two diploid Triticum indicated a clear interspecific divergence, the two tetraploid wild wheats were not clearly divergent in this study. The variance portioning analysis indicated that the variation detected for diploid Triticum species was mainly composed of `between species' variation, on the other hand that for tetraploid Triticum was mostly composed of `within population' variation. In conclusion, AFLP analysis reveals molecular variation in all accessions used in this study, suggesting a potential genetic diversity of the wheat wild relatives in natural populations. These results have implications for the design of strategies to maintain genetic diversity within genebank collections. This revised version was published online in August 2006 with corrections to the Cover Date.  相似文献   

9.
Summary In this study genetic variation for resistance to the greenhouse whitefly (Trialeurodes vaporariorum) of four genotypes of tomato (L. esculentum) and two subspecies of L. hirsutum was investigated. Resistance was quantified by the whitefly life history components adult survival, oviposition rate, pre-adult survival and developmental period, measured on plants inoculated with whiteflies in clip-on cages.The largest differences between species were found when life history components were measured on adult plants of about four months old. On L. hirsutum f. glabratum whiteflies had the lowest adult survival, oviposition rate and pre-adult survival. On L. hirsutum these components were intermediate whereas on all L. esculentum genotypes they were highest. The variation between plants was low compared to the variation within plants. These results indicate that single plant tests can be used to determine accurately genetic variation between individual plants in a segregating population.  相似文献   

10.
Summary Various Cuphea species are adapted to temperate climates and characterized by caprylic, capric, and lauric acid-rich seed oils. These fatty acids are solely commercially available from palm kernel and coconut oils; thus, there is considerable interest in Cuphea as an alternative source of these fatty acids. Cuphea is a genus of undomesticated species characterized by seed dormancy and seed shedding. Our goal is to eliminate these domestication barriers in a few species with agronomic potential. Our objective was to survey existing Cuphea laminuligera and C. lanceolata germplasm for genetic variation for seed dormancy (germination percentage after a certain seed storage period). Recurrent half-sib family selection was practiced in various open-pollinated and synthetic populations. Half-sib family seed were evaluated for germination percentages at 26° C after various seed storage periods. There was significant genetic variation among families in every population. Additive genetic variance, half-sib family-mean heritability, and expected selection response magnitudes depended on the length of the seed storage period within a given population. Estimates of these parameters were significantly greater for longer seed storage periods within a population. Heritability estimates ranged from 0.00 to 0.79. Expected half-sib family selection responses ranged from 0.0 to 23.9%. Our study demonstrated there is substantial genetic variation for seed dormancy in C. laminuligera and C. lanceolata.  相似文献   

11.
Y. Hébert 《Euphytica》1990,46(3):237-247
Summary Evidence of genetic variation for early vigour is presented using maize line × tester crosses. The leaf appearance rate and associated variance components are affected by a physiological stress attributable to the transition to autotrophic nutrition by the plant. At this stage, specific combining ability plays an important role in the total genetic variation. It is concluded that differing genetic controls exist in early and late material, and that this difference is also manifested in leaf initiation and elongation rates. The ground coverage rate, as a component of plant development, is genetically correlated to total dry matter yield. The genetic correlations vary according to the growth stage.  相似文献   

12.
Summary Twelve spring wheat cultivars were grown as isolated plants in the field and their pre-anthesis growth was interpreted in terms of plant growth analysis. Relative growth rate (RGR) decreased steadily with time due to a decline of leaf area per unit plant weight (LAR), which could be explained by the reduction of the portion of leaf weight in total plant weight (LWR). Growth per unit leaf area (NAR) and leaf area per unit leaf weight (SLA) changed only little with time.Differences between cultivars for NAR and LAR were of similar magnitude: both 8% when measured by the genetic coefficient of variation. Because both quantities were negatively correlated, the genetic variation of RGR was only 5%. Genetic variation for LWR and SLA were also of similar size, both about 4%. Estimates of genetic variances and covariances based on cultivar means appeared to be biased strongly when the error variation of the means was neglected.Special attention is paid to the methodology of plant growth analysis.  相似文献   

13.
Outi Savolainen 《Euphytica》1996,92(1-2):139-145
Summary Plant populations in extreme conditions differ from those in more favorable conditions in many respects. They have developed special physiological adaptations for withstanding e.g. extreme temperatures or drought. On the other hand, low survival and fertility may cause smaller effective population sizes, which in turn influences patterns of genetic variation. Scots pine has the widest range of all pine species, extending in the north far beyond the arctic circle. Close to the tree limit, the growing season is short and survival is low. Very northern populations also have lower and more irregular male and female flowering and seed production than southern ones. The consequences of these conditions on genetic variation have been examined. All Finnish populations have similar patterns of variation at allozyme loci and nuclear DNA markers: high variation but no differentiation between populations. Northern populations seem to have slightly fewer recessive deleterious alleles than southern ones. There is, however, strong differentiation between populations with respect to adaptation to the very short growing season. In common garden experiments, northern populations cease growth and set buds much earlier and develop frost tolerance earlier than southern populations. The genetic basis for this high differentiation in bud set can be explored by using dense maps of molecular markers. The implications of these patterns of variations on conservation of genetic resources and tree breeding will be discussed.  相似文献   

14.
Tissue culture-derived variation in crop improvement   总被引：30，自引：3，他引：30  
S. Mohan Jain 《Euphytica》2001,118(2):153-166
Tissue culture generates a wide range of genetic variation in plant species which can be incorporated in plant breeding programmes. By in vitro selection, mutants with useful agronomic traits, e.g. salt or drought tolerance or disease resistance, can be isolated in a short duration. The successful use of somaclonal variation is very much dependent on its genetic stability in the subsequent generations for which molecular markers such as RAPDs, AFLPs, SSRs and others can be helpful. The potential of somaclonal variation has yet to be fully exploited by breeders, even though a few cultivars have been developed in crops such as Brassica juncea, rice and others. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

15.
RAPD (randomly amplified polymorphic DNA) analyses were carried out on 21 accessions of Lachenalia bulbifera (Cyrillo) Engl. Five pre-selected primers produced an average of 88% polymorphisms. Fifteen of the 21 accessions could be identified using the five primers. In a pairwise comparison genetic distance values ranging from 0.11 to 1.08 were obtained. These values reveal a high amount of variation within the species. The genetic distance values within the tetraploid and hexaploid groups on the south coast were low, but values were high between the groups on the south coast and those on the west coast. A dendogram was constructed from the RAPD banding profiles, using UPGM cluster analysis. The dendogram clusters certain accessions together. These clusters are supported by their geographical locality and chromosome data. The hexaploid group, tetraploid group and octoploid group on the south coast are respectively clustered together. It is concluded that RAPDs can be used to assess the genetic variation at an intra-specific level in Lachenalia. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

16.
C. J. Liu 《Euphytica》1996,90(1):115-119
Summary Genetic variation in 40 accessions of Lablab purpureus was evaluated using random amplified polymorphic DNA as markers. A high level of genetic variation in this species was detected but this was mainly restricted to the difference between cultivated and wild forms. Of the cultivated genotypes, genetic variation among Asian collections was significantly higher than that among African collections. The three most divergent cultivated genotypes were all from Asia. Four of the five wild accessions, two from Zimbabwe and the other two from Zambia, were closely related. The other one, CPI 31113 collected from Uganda, was highly divergent. The two commercial forage varieties used in Australia, Rongai from Kenya and Highworth from India, were not very different.  相似文献   

17.
Summary Ethiopian landraces of tetraploid wheat can be grouped according to their seed colors in three major groups: brown, purple and white seeded. Seeds with different colours are used for different purposes, and the three seed colour groups are treated separately in breeding programs. The genetic variation between and within these groups was studied by isozyme analyses at six highly polymorphic loci in sixty landrace agrotypes. The mean number of alleles per locus was 1.95. The mean allele frequencies showed significant variation both within and between the seed colour groups. The brown and white seeded types had very high genetic identity and the genetic identity values between the brown and purple and the purple and white groups were only slightly smaller. The average coefficient of gene differentiation between the seed colour types was very low. Only about 5% of the total genetic variation was due to differences between the seed colour groups. This indicates that agrotypes of different seed colours can not be treated as genetically separate and distinct groups.  相似文献   

18.
Oleander is a Mediterranean evergreen shrub found along watercourses, gravelly places and damp slopes. It is grown widely as an ornamental for its abundant and long-lasting flowering as well as its moderate hardiness. Genetic relatedness among 71 accessions, including commercial varieties, different sources of the same varieties, and selections from the wild were investigated using amplified fragment length polymorphism (AFLP). Nine primer combinations yielded a total of 603 bands of which 241 were polymorphic. Genetic similarities among accessions were calculated according to Jaccard's Similarity Index and used to construct a dendrogram based on the unweighted pair group method using arithmetic averages. Our results show that the AFLP technique, which can simultaneously and assay a large number of loci randomly distributed in the genome, is much more informative on the genetic relationship and origin of accessions than the limited number of morphological characters conventionally used for variety discrimination. Up to about 9% molecular genetic differentiation was detected among morphologically indistinguishable provenances of the same variety; this can be partly attributed to scoring error but mainly to somatic variation occurring during vegetative propagation. On the other hand lower genetic distance values were sometimes found among varieties which differ in morphological characters and are thus commercialised with different names. The possibility of considering the amount of genetic variation within a variety as the threshold value for discrimination of initial varieties and essentially derived varieties is discussed. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

19.
Summary Wild relatives are valuable genetic resources for crop improvement. Evaluating genetic variation in these species is not only important for their use in breeding programs, but will also provide information about evolution of crops. Seeds representing six natural populations were used to study the level of variation in the South Korean wild soybean. Electrophoretic assays of the seeds on horizontal slab gels were conducted to determine the genotypes of each natural plant at 35 loci in 17 isozymes and one protein. The results indicated a surprisingly high variation. The number of alleles at each locus was as high as four. Seventy two of the 94 reported alleles for the 35 loci were present in these populations. The average number of alleles per locus, 99% polymorphism and the expected heterozygosity in the total population were 2.1, 77.1% and 0.215, respectively. This amount of variation was not only higher than that reported for 857 soybean cultivars and wild soybean populations from other geographic regions, but also higher than the average for 123 self-fertilized plant species and 473 plant species of all mating systems. The high variation in the South Korean wild soybean as well as cultivated soybean indicated in this and other population genetic studies prompts us to propose that South Korea is one of the major soybean gene centers.  相似文献   

20.
Breeding of cut and pot rose cultivars for efficient production under low-energy conditions in greenhouses will be facilitated by understanding the inheritance of vigour. To get insight into the genetic variation of vigour-related traits, a diploid rose population was employed for an evaluation study in greenhouses in The Netherlands and Denmark. For all the traits investigated the population showed a continuous quantitative variation as well as a considerable transgression. For most of the traits, the genetic variation found among the tested entries was highly significant and tended to be large in comparison to the effects of genotype by environment interaction. The heritability based on means of the traits was high and ranged from 68 to 92%. Strong simple correlations (r = 0.65 to 0.95) were found among the traits shoot length, leaf area, leaf dry weight, stem dry weight, total dry weight and growth rate. The total dry weight and leaf area are suggested to be good parameters for early selection of rose genotypes with vigorous growth under suboptimal growth conditions.  相似文献   

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