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硫酸粘杆菌素微生物检定法的改进 总被引：5，自引：1，他引：4
本文以大肠杆菌标准菌株(Escherichia coli CMCC(B)44103)为检测菌,对硫酸粘杆菌素的微生物检测方法进行了研究.研究结果表明,本方法检测限为0.2μg/ml,与国外所用的以博德特氏菌ATCC4617为检测菌的检测限基本一致,其质量控制样品的日内、日间变异系数均小于10%,平均回收率94%～100.8%,并且操作简便,是一种较好的替代方法,可用于硫酸粘杆菌素的药代动力学研究或进行残留检测. 相似文献
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1发病情况 2004年9月16日,同心县丁家塘乡丁某等4个养鸡户从孵化户徐某处收入1日龄肉杂鸡2000只,在土炕或平网上育雏(上面铺旧报纸),育雏室温度35～36℃,相对湿度60%～68%,饲喂正大肉雏鸡喂料.19日开始,4户的雏鸡均出现零星死亡,当地兽医诊断后给予了青霉素饮水和多维电解质饮水,但用药效果不明显.21日死亡数量逐渐增加,继续用药后仍不能控制病情,23日前来我市兽医站进行诊断.经调查,4户共死亡雏鸡84只,死亡率4.2%,其中1户最多死亡33只,死亡率达8.25%(33/400).根据畜主提供的情况,同时对孵化场进行了追踪调查. 相似文献
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黄土高原长期种植苜蓿对土壤硫、钙、镁的影响 总被引：1，自引：0，他引：1
以22年定位试验为基础,研究黄土高原长期种植紫花苜蓿(Medicago sativa L.)情况下土壤硫、钙、镁组分的变化。结果表明:长期施P使苜蓿连作耕层土壤总硫、有效硫、水溶性硫、吸附性硫、HCl可溶性硫、总无机硫和有机硫含量分别增加9.41%、62.41%、47.51%、30.07%、2.25%、5.38%和17.54%;长期施NPM使苜蓿连作耕层土壤总硫、有效硫、水溶性硫、吸附性硫、HCl可溶性硫、总无机硫和有机硫含量分别增加20.89%、98.31%、68.44%、57.34%、8.07%、12.54%和37.69%。长期施P使苜蓿连作耕层土壤全钙、有效钙、水溶态钙、交换态钙和酸溶态钙含量增加分别增加4.64%、4.27%、11.66%、4.05%和8.59%,但残余态钙含量降低2.21%;长期施NPM使苜蓿连作耕层土壤全钙、有效钙、水溶态钙、交换态钙和酸溶态钙含量增加分别增加8.69%、8.30%、51.59%、6.73%和27.77%,但残余态钙含量降低26.23%。长期施NPM使苜蓿连作耕层土壤全镁、有效镁、水溶态镁、交换态镁、酸溶态镁和残余态镁含量分别增加7.38%、61.98%、63.16%、61.85%、5.81%和8.97%。此外,有效硫在土壤剖面上出现双累积峰,有向深层土壤迁移的特征;有效钙、有效镁在土壤剖面上存在淋溶累积现象。此研究结果可为苜蓿种植和管理提供依据。 相似文献
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猪用免疫球蛋白对一起猪瘟防治效果的观察 总被引：1，自引：0，他引：1
在猪群发生猪瘟的紧急情况下,根据不同病程,应用猪用免疫球蛋白和猪瘟活疫苗进行紧急防治.效果观察结果表明,对从病猪群中分出的健康猪群,及时注射猪用免疫球蛋白,并在第三天注射猪瘟活疫苗,可阻断或消除病原,控制传染病在健康猪群中的蔓延,有100%的保护率.而只采用猪瘟免疫球蛋白,对病程短、只有体温升高而无其它临床表现的猪,治愈率100%;对病程较长、已表现出猪瘟临床症状的,治愈率只有37.5%.对上述同样病程的病猪只采用猪瘟活疫苗注射,治愈率分别为40%和0. 相似文献
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用高效液相色谱法同时测定呼泻泰乐水溶性粉中酒石酸泰乐菌素、盐酸多西环素、甲氧苄氨嘧啶乳酸盐的含量,色谱柱用C18柱(5μm,3.9mm×250mm),高氯酸钠119g加35%乙腈溶液1000ml制成0.85mol/ L的溶液(用1mol/ L盐酸溶液调节pH值至2.5±0.1)为流动相,检测波长272nm,流速1.0ml/ min,进样量20μl.三组分的回收率分别为99.6%±2.3%、100.0%±1.7%、99.3%±1.8%,线性范围分别为0.16～0.8mg/ ml、0.16～0.8mg/ ml、0.04～0.2mg/ ml,相关系数分别为0.9997、0.9998、0.9998.本方法操作简便、快捷,分离度好,结果准确可靠. 相似文献
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《蚕业科学》2015,(6)
多糖是家蚕幼虫体内富含的活性成分之一,为了建立高效的蚕体水溶性粗多糖提取工艺,以家蚕5龄第3天幼虫为材料提取水溶性粗多糖。在单因素试验的基础上,采用Box-Behnken设计对提取温度、提取时间和原料质量浓度3个因素进行中心组合试验,研究各因素交互作用及对蚕体水溶性粗多糖提取率的影响,采用Design-Expert软件拟合得到预测提取率的二次回归方程。试验结果表明,提取时间和原料质量浓度的交互作用对蚕体水溶性粗多糖提取率具有极显著影响,3个因素对提取率贡献大小的排序为原料质量浓度提取时间提取温度。运用响应面法优化得到最优提取工艺条件为:提取温度80℃,提取时间97 min,原料质量浓度37.5 g/L。优化的提取工艺简单、稳定,蚕体水溶性粗多糖的实际提取率为3.042%,与预测值(3.055%)非常接近,具有应用价值。 相似文献
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Evidence for the non-involvement of the uterus in the lifespan of the corpus luteum in the cyclic dog 总被引：1，自引：0，他引：1
Progesterone levels in peripheral blood of dogs were analysed during the cycle in which hysterectomy (n = 5) or sham surgery (n = 3) was performed as well as during the cycle of dogs (n = 5) hysterectomized at least one year prior to this study; the data were compared with the findings in control dogs (n = 3). The averages of the duration of the luteal period observed in the three experimental groups were not significantly different from those of control dogs. Immediately after surgery, the progesterone level decreased from 25 to 50% of the presurgical level, but returned to presurgical level in about four days. Prolactin levels were elevated for about 30 h after surgery. Nevertheless, the averages of the mean prolactin levels for each animal during the luteal period of the experimental groups were not significantly different from those of control dogs. It is concluded that in the dog, the uterus is not involved in the lifespan of the cyclic corpus luteum. 相似文献
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嗅源是军犬嗅觉作业训练的依据.军犬鉴别中嗅源能力的培养在整个鉴别训练中占有重要地位,关系到鉴别训练的成败和鉴别能力的提高.所以,军犬在嗅认嗅源时必须按照训犬员的指令充分、细致地感受嗅源气味,并且对嗅源气味产生兴奋反应.如何培养军犬充分嗅认嗅源以及及时解决嗅认过程中出现的问题,成为我们进行鉴别训练的关键.…… 相似文献
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Meschan EM Peham C Schobesberger H Licka TF 《Veterinary journal (London, England : 1997)》2007,173(3):578-584
As there is no statistical evidence that saddle fit influences the load exerted on a horse's back, this study was performed to assess the hypothesis that the width of the tree significantly alters the pressure distribution on the back beneath the saddle. Nineteen sound horses were ridden at walk and trot on a treadmill with three saddles differing only in tree width. Kinetic data were recorded by a sensor mat. A minimum of 14 motion cycles were used in each trial. The saddles were classified into four groups depending on fit. For each horse, the saddle with the lowest overall force (LOF) was determined. Saddles were classified as "too-narrow" if they were one size (2 cm) narrower than the LOF saddle, and "too-wide" if they were one size (2 cm) wider than the LOF saddle. Saddles two sizes wider than LOF saddles were classified as "very-wide". In the group of narrow saddles, the pressure in the caudal third (walk 0.63 N/cm(2)+/-0.10; trot 1.08 N/cm(2)+/-0.26) was significantly higher compared to the LOF saddles (walk 0.50 N/cm(2)+/-0.09; trot 0.86 N/cm(2)+/-0.28). In the middle transversal third, the pressure of the wide saddles (walk 0.73 N/cm(2)+/-0.06; trot 1.52 N/cm(2)+/-0.19) and very-wide saddles (walk 0.77 N/cm(2)+/-0.06; trot 1.57 N/cm(2)+/-0.19) was significantly higher compared to LOF saddles (walk 0.65 N/cm(2)+/-0.10/ 0.63 N/cm(2)+/-0.11; trot 1.33 N/cm(2)+/-0.22/1.27 N/cm(2)+/-0.20). This study demonstrates that the load under poorly fitting saddles is distributed over a smaller area than under properly fitting saddles, leading to potentially harmful pressures peaks. 相似文献
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Observations on the Pathology of the Spleen of the Cat 总被引：1，自引：0，他引：1
Abstract— Lesions were found in the spleen of four of sixty-three cats examined post mortem in the department, and comprised two examples of lymphosarcoma, one of chronic fibrinous peritonitis and one of subcapsular haematoma. Abnormalities were found in two cat spleens received from veterinary surgeons in general practice. In one cat the abnormalities were an haemangiosarcoma with secondary deposits in the liver and in the other extensive mast cell infiltration. In addition lesions were detected in the spleen of three of eighty-seven cats examined post mortem in general practice. The lesions were: multiple secondary deposits of adenocarcinoma from a primary tumour of the mammary gland, a ruptured spleen and a foreign body below the splenic capsule. Résumé— L'examen microscopique de soixante-trois chats pratiqué dans le service a permis de rencontrer quatre fois des lésions de la rate, dont deux lymphosarcomes, une péritonite capsulaire fibrineuse et un hématome sous-capsulaire. D'autre part des lésions ont été trouvées dans deux rates de chat expédiées par des vétérinaires exerçant en pratiaue générale. Chez un chat il s'agissait d'un htmangiosarcome avec métastases hépatiques et chex l'autre d'une infilt ation diffuse par des mastocyta. De plus, on a rencontré des llésions dnas la rate de trois chats parmi quatre-vingt sept autopsies effectuées dans le cadre d'une clientèle génárale. Ces iésions étaient dés métastases multiples d'un adénocarcinome dont la tumeur primitive siègeait dam la glande mammaire, une rupture de la rate et un corps érangers sou capsulaire. Zusammenfassung— Läsionen der Milz wurden bei vier von dreiuiidsechzig in dieser hbteilung sezierten Katzen gefunden, und zwar in zwei Fällen ein Lymphosarkom, in einem Falle eine chron-ische fibrinöse Peritonitis und in einem ein subkapsuläres Hématom. Anomalien fanden sich auch in der Milz von zwei Katzen, die aus der tierxrztlichen Praxis eingesandt wurden. Bei der einen Katze war dies ein Hämangiosarkom mit Metastasen in der Leber, bei der anderen eine weitläufige Infiltration der Mastzellen. Außerdem wurden bei drei von siebenundachtzig in der Praxis sezierten Katzen Milzläsionen gefunden. Diese bestanden am multiplen Metastasen eines Adenokarzinoms der Brustdrüse, einer Milzzerreissung und einem Fremdkörper unterhalb der Milzkapsel. 相似文献