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1.
The effects of salinity on haemolymph osmolality, oxygen consumption and ammonia excretion were investigated in adult kuruma shrimp, Marsupenaeus japonicus (Bate), at salinities of 20, 25, 30 and 35 g L?1. Haemolymph osmolality showed a positive linear relationship (r2=0.9854) with medium osmolality. The isosmotic point calculated from this relationship was 1039 mOsm kg?1, which corresponds to a salinity of approximately 35 g L?1. The slope of the regression equation was very high (0.81), suggesting that M. japonicus adults are poor osmoregulators compared with the adults of other penaeids and to conspecific young. The difference between haemolymph and medium osmolality (DOP) was lowest at 35 g L?1 and highest at 20 g L?1. Thus, the minimum DOP coincided with the isosmotic point of the shrimp. The respiration rate was significantly lower at 30 g L?1 than at the other salinities. Ammonia excretion rates were inversely related with salinity and therefore were minimal at 35 g L?1. The results of this study suggest that the optimum salinity for adult kuruma shrimp is around 30–35 g L?1 and that even minor (e.g. 5 g L?1) deviations from this optimum cause significant physiological changes. Further, the observed increases in oxygen consumption and ammonia excretion during exposure to low salinities, which indicate higher energy expenditure and amino acid catabolism for osmoregulation, respectively, suggest that the growth efficiency of M. japonicus adults may be severely compromised by hypohaline water inflow into the rearing ponds.  相似文献   

2.
The aim of this study was to evaluate the effect of light limitation on the water quality, bacterial counts and performance of Litopenaeus vannamei postlarvae reared with biofloc at low salinity (≈9 g L?1). Two treatments were designed: T1 = culture with natural sunlight and T2 = culture in darkness. After 28 days, in both treatments, the final weight of shrimp was over 0.6 g with a specific growth rate over 7.4% d?1, and a survival rate over 70%. In both treatments, Vibrio sp. concentration presented low values (culture with natural sunlight = 0.1 to 9.9 × 102 CFU mL?1, culture in darkness = 0.4 to 11.7 × 102 CFU mL?1) and Bacillus sp. had high values (culture with natural sunlight = 0.7 to 66.0 × 104 CFU mL?1, culture in darkness = 0.7 to 65.8 × 104 CFU mL?1). All water quality parameters remained within the ranges suitable for shrimp culture, except for alkalinity during the first stage of the study. Although in some sampling periods some significant differences were found in bacterial counts and water quality parameters, shrimp productive performance under culture with biofloc at low salinity was not affected significantly by light limitation.  相似文献   

3.
The oxygen consumption (02) and ammonia excretion (N) of juvenile and adult common wolffish was measured in culture tanks in the laboratory. The oxygen consumption and ammonia excretion were affected by temperature (C), fish size and feeding rate. For juveniles (0.5 kg; 7C) the diel oxygen consumption rate varied between 37 and 62 mg O2 kg-1 h-1 and ammonia excretion between 2.3 and 5.7 mg N kg-1 h-1. The corresponding rates for adult fish (6.9 kg; 7C) were 29-44 mg O2 kg-1 h-1 and 1.2–3.1 mg N kg-1 h-1. The weight-specific oxygen consumption (mg O2 kg-1 h-1) was described by the following formulae: O2 (7C) = 0.17* W0.83 and O2 (12C) = 0.39 * W0.73 and the corresponding ammonia excretion (mg N kg-1 h-1) by: N(7C) = 0.024 W0.75 and N(12C) = 0.073 W0.60, where W is fish weight in g.  相似文献   

4.
An experiment was conducted to study the effect of protein level on the growth rate, survival, pre- and postprandial oxygen consumption, and ammonia (N-NH3) excretion in white shrimp. Penaeus setiferus (L.), juveniles. Diets containing 10%, 20%, 30%, 40% and 50% protein were provided at a proportion of 15% of the dry weight of shrimp weighing 0.19 ± 0.01g. The instantaneous growth rate was recorded for 45 days. The optimum protein level, in terms of the best growth rate, was that of the 30% protein diet (1.1 5g45days?1). All shrimp fed with the diets had significantly higher respiration rates after feeding because of the apparent heat increment (AHI). A greater postprandial nitrogen excretion (PPNE) excretion was also observed after feeding. Shrimp maintained on the 30% protein diet had the lowest AHI and PPNE coefficients (percentage of ingested energyl. and took the least time to reach the oxygen consumption and nitrogen excretion peak. The 0:N ratios varied according to protein level, with higher values (32 and 44, fasting and Feeding, respectively) with a 30% protein diet, and lower values (10 and 13. fasting and feeding, respectively) with 10% and 50% protein diets. These results indicate that P. setiferus juveniles use protein when fed with high (50%) and low (10%) protein diets, and lipid-protein substrates with a 30% protein diet. Under these conditions, P. setiferus grow better with a 30% protein diet where shrimp use food more efficiently.  相似文献   

5.
The experiment was conducted with three biofloc treatments and one control in triplicate in 500 L capacity indoor tanks. Biofloc tanks, filled with 350 L of water, were fed with sugarcane molasses (BFTS), tapioca flour (BFTT), wheat flour (BFTW) and clean water as control without biofloc and allowed to stand for 30 days. The postlarvae of Litopenaeus vannamei (Boone, 1931) with an Average body weight of 0.15 ± 0.02 g were stocked at the rate of 130 PL m?2 and cultured for a period of 60 days fed with pelleted feed at the rate of 1.5% of biomass. The total suspended solids (TSS) level was maintained at around 500 mg L?1 in BFT tanks. The addition of carbohydrate significantly reduced the total ammonia‐N (TAN), nitrite‐N and nitrate‐N in water and it significantly increased the total heterotrophic bacteria (THB) population in the biofloc treatments. There was a significant difference in the final average body weight (8.49 ± 0.09 g) in the wheat flour treatment (BFTW) than those treatment and control group of the shrimp. Survival of the shrimps was not affected by the treatments and ranged between 82.02% and 90.3%. The proximate and chemical composition of biofloc and proximate composition of the shrimp was significantly different between the biofloc treatments and control. Tintinids, ciliates, copepods, cyanobacteria and nematodes were identified in all the biofloc treatments, nematodes being the most dominant group of organisms in the biofloc. It could be concluded that the use of wheat flour (BFTW) effectively enhanced the biofloc production and contributed towards better water quality which resulted in higher production of shrimp.  相似文献   

6.
We investigated the effects of the stocking density of white shrimp (Litopenaeus vannamei) on shrimp and tilapia growth and nutrient conversion in an integrated closed recirculating system both with and without Nile tilapia (Oreochromis niloticus). A 2 × 3 factorial design involving tilapia presence/absence and shrimp stocking densities of 40, 80 and 120 m?2 was applied, using a tilapia:shrimp ratio of 0.025. There were no significant interactions between tilapia presence and shrimp stocking density in terms of shrimp growth performance or feed utilization. The presence of tilapia had no effect on the shrimp growth rate, survival rate or total weight gain (%). Shrimp growth declined significantly with increased shrimp stocking density, but the growth of tilapia was not significantly different among the three shrimp densities tested. The conversion of feed nitrogen and phosphorus into total harvested animal biomass was significantly higher in the presence than in the absence of tilapia. The nutrient conversion rate at the lowest shrimp density (40 m?2) was significantly higher than at the highest density tested (120 m?2).  相似文献   

7.
The present study aimed to determine the effect of feeding time on growth and nitrogen excretion in juvenile sole. An 84‐day growth trial was conducted, in which food was supplied to three triplicate groups of juvenile Senegalese sole (3 g wet weight) at different schedules – diurnal, nocturnal and mixed. At the end of the growth trial, ammonia and urea excretion was assessed during a 24 h cycle. Improved growth (1.3% vs. 0.9% day?1, specific growth rate), higher nitrogen retention (0.35 vs. 0.27 g N kg?1 day?1), lower ammonia excretion (209 vs. 272 mg N‐NH4 kg?1 day?1) and lower total nitrogen excretion (278 vs. 352 mg N kg?1 day?1) were found in daytime‐fed fish compared with night‐fed fish. Fish in the mixed feeding regime showed intermediate values of ammonia and total nitrogen excretion, but did not differ from day‐fed fish regarding the other parameters. Results indicate that juvenile sole at a period of their life cycle appear to use more efficiently dietary protein for somatic growth under a diurnal than under a nocturnal feeding regime. This suggest that at least during a time‐window in the juvenile rearing a diurnal feeding regime might be more effective in the production of this species.  相似文献   

8.
The pink shrimp Farfantepenaeus brasiliensis is native in southern Brazil and is potentially suited for aquaculture. Under intensive culture, the accumulation of nitrogenous compounds results from excretion by the shrimp and from the processes of feed decomposition and nitrification. The objective of this study was to evaluate ammonia, nitrite, and nitrate toxicity effects on oxygen consumption of juvenile pink shrimp. Shrimps (initial weight 0.7 ± 0.15 g) were exposed over a period of 30 days to 50%, 100%, and 200% of the safe levels of total ammonia (TAN = 0.88 mg/L), nitrite (NO2? = 10.59 mg/L), and nitrate (NO3? = 91.20 mg/L) for the species. The specimens were individually collected and placed in respirometry chambers, where the oxygen consumption was measured over a period of two hours. Throughout the experiment there was no significant difference (p > 0.05) among treatments in terms of survival and growth. The pink shrimp juveniles exposed to nitrogen concentrations of 200% of the nitrite and nitrate safe level showed the highest oxygen consumption (p < 0.05).  相似文献   

9.
An indoor trial was conducted for 28 days to evaluate the effects and interactions of biofloc and seaweed Ulva lactuca in water quality and growth of Pacific white shrimp Litopenaeus vannamei in intensive system. L. vannamei (4.54 ± 0.09 g) were stocked in experimental tanks at a density 132 shrimp m?2 (566 shrimp m?3) and the U. lactuca was stocked at a density 0.46 kg m?2 (2.0 kg m?3). Biofloc with seaweed (BF-S) significantly reduced (P < 0.05) total ammonia nitrogen (TAN) by 25.9 %, nitrite–nitrogen (NO2–N) by 72.8 %, phosphate (PO 4 3 -P) by 24.6 %, and total suspended solids by 12.9 % in the water and significantly increased (P < 0.05) settleable solids by 34.2 % and final weight of shrimp by 6.9 % as compared to biofloc without seaweed. The BF-S can contribute by reducing nitrogen compounds (TAN and NO2–N), phosphate (PO 4 3 -P), and total suspended solids in water and increased final weight of shrimp.  相似文献   

10.
The objective of this study was to assess zootechnical and physiological performance of Litopenaeus vannamei postlarvae (PL) reared in three environments (CW, clear water; B, biofloc; BS, biofloc with artificial substrates) at three stocking densities (300, 600, 900 PL/m3) for 8 weeks. At the end of experimentation, shrimp were subjected to hypoxia, and physiological response was again assessed. During rearing, low levels of total ammonia nitrogen, nitrite (NO2?) and nitrate (NO3?) were observed in B and BS for 600 and 900 PL/m3. For 300 PL/m3, a slight accumulation of NO2? and NO3? was detected. For the same stocking density, shrimp reared in B and BS showed significantly higher weights than those grown in CW, except for final weight. No significant differences were observed in survival. The use of biofloc and artificial substrates permitted doubling density from 300 to 600 PL/m3 without affecting growth, survival, feed conversion rate and obtaining twice the biomass. Shrimp grown in B and BS stored a surplus of glycogen and carbohydrates in their hepatopancreas, which probably gave them a better physiological capacity to counteract high‐stocking densities and hypoxia. A tendency of a higher adenylate energetic charge was observed in shrimp maintained in B and BS.  相似文献   

11.
A feeding trial with five crude protein levels (549, 513, 472, 445 and 399 g kg?1) was conducted to investigate the optimum protein level in diets of juvenile southern flounder (Paralichthys lethostigma). Budgets of nitrogen and energy were discussed. Fish (initial weight 32.9 ± 0.5 g fish?1, mean ± SD) were fed the experimental diets to satiation twice daily for 61 days. Protein levels affected specific growth rate in wet weight (SGRW) and protein (SGRP) significantly. SGRW and SGRP were highest at 512.5 g kg?1 protein level. SGRW was positively correlated to growth nitrogen (GN), growth energy (GE), nitrogen digestibility, energy digestibility, amount of digestible nitrogen and amount of digestible energy. Faecal nitrogen (FN) and faecal energy (FE) were affected significantly with trends contrary to SGRW. The nitrogen budget was described by the equation 100CN = 2.1FN + 34.4UN + 63.5GN (CN, nitrogen intake; UN, excretion nitrogen). The energy budget was 100IE = 4.04FE +3.32UE + 54.35GE + 38.30ME (IE, gross energy intake; UE, excretion energy; ME, metabolizable energy). The average proportion of GE and ME in assimilated energy (AE) was described by the equation 100AE = 58.65GE + 41.35ME.  相似文献   

12.
Effects of temperature on food consumption, growth and oxygen consumption were estimated for the freshwater prawn Macrobrachium rosenbergii postlarvae at 23 °C, 28 °C and 33 °C in the laboratory. The results showed that the animal's initial body weight had a close linear relationship with food consumption and growth. Food consumption increased directly with temperature. Consumption rates (C; mg day?1 ind?1 ) of the 28 °C and 33 °C groups were much higher than that of the 23 °C group (P < 0.001), and the 33 °C group's consumption rate was higher than that of the 28 °C group (P < 0.05). The relationship of food consumption with temperature and initial body weight (W; mg) could be described as: C = 0.0679W + 0.185t? 3.17. Growth increased significantly with increased temperature. The relationship among specific growth rate, temperature and initial body weight was as follows: SGR = ?0.110W + 0.213t + 0.176. However, temperature showed no effect on growth efficiency. Oxygen consumption increased significantly with temperature (P < 0.01). The weight‐specific oxygen consumption rates (mg O2 g?1 h?1) at 23 °C, 28 °C and 33 °C were 0.83, 1.16 and 1.49 mg O2 g?1 h?1 for 61.92 mg M. rosenbergii.  相似文献   

13.
Three components of the energy budget, consumption (C), respiration (R) and growth (G). were measured in larval and early juvenile sole (77 μg to 7.2 mg dry weight) kept under controlled temperature (15oC), light (photoperiod 18 h at 800 lux) and food (Artemici salina. 5 ind ml-1) conditions. The increase in body weight with age is described by the growth function Wt= 51.9 e0143t, where t is time in days from hatching. Wt is weight (ug dry wt) at time t, 51.9 is the estimate of larval weight at hatching (Wo) and 0.143 is the instantaneous growth coefficient. Daily food consumption (C, in ug dry wtday”1 ind-1) increased with body weight (W, in ug dry wt) according to the allometric function, C = 1.852W0.852. Low routine oxygen consumption (R, in nl 02 h-1 ind-1) was best described in relation to body weight by the allometric equation R = 1.47W0.975 By using the above growth and allometric functions and by converting G, C and R into their energetic equivalents (joules day-1 ind-1), the changes in assimilation efficiency (K1) and gross growth efficiency (K1) during ontogeny could be modelled.  相似文献   

14.
The hypothesis that intraspecific competition affects survival and growth during the culture and harvest at extensive/semi-intensive Penaeus vannamei shrimp ponds was evaluated. Thus, the effect of stocking density on the biomass, shrimp average weight, survival, and economic performance during the culture (133 days) and at the harvest of the P. vannamei shrimp was investigated in 400 m2 earthen ponds. In order to reduce the likelihood of infectious diseases, shrimp received preventive health treatments (probiotics and β-1,3/1,6-glucans) during all culture phases. In this way, the effect of density on the intraspecific competition for space/food was isolated. Ponds stocked at 6, 9, and 12 shrimp m?2 showed competition-dependent growth. Ponds stocked at 12 shrimp m?2 presented a mortality (12 %) between days 76 and 99. Competition, and accordingly individual growth reduction, could have begun at day 76 at a density of 5 shrimp m?2. Survival was significantly higher at 6 shrimp m?2 (84.2 ± 6.2 %) compared with the 12 shrimp m?2 (64.8 ± 12.4 %) treatment, while no significant differences in yield were observed between both treatments. Ponds stocked at 3 and 6 shrimp m?2 presented the best benefit–cost rates. The optimal shrimp density during the experimental culture was 5 shrimp m?2. Given the experimental conditions and considering the fraction of density-independent mortality observed, the optimum stocking density was found to be 6 shrimp m?2.  相似文献   

15.
Galaxias maculatus is an osmeriform native fish of the Southern Hemisphere, in which the crystalline larvae is considered as a luxury delicacy, for this reason, it has been commercially exploited in Chile, Argentina and New Zealand. However, the fisheries have been rapidly decreasing due to the overexploitation and the predation of introduced species. Because of these events, there is a need to determine a carrying capacity for an intensive fish culture. In order to optimize stocking densities for fish culture, this paper proposes objectives to determine oxygen consumption (OC) rates, dissolved oxygen concentrations that produce signs of hypoxia and the average time elapsed between food intake and peak OC in G. maculatus. In the oxygen experiments under routine metabolism conditions, we found that G. maculatus adults and whitebait showed signs of asphyxia at dissolved oxygen concentrations between 1.3 and 2.2 mg L?1 and that adults tolerated dissolved oxygen levels as low as 1.3 mg L?1. The results showed that G. maculatus individuals with an average weight of 0.04 g consumed 0.048 mg O2 h?1, whereas individuals with an average weight of 1.4 g consumed 0.345 mg O2 h?1. Galaxias maculatus increased the OC rate by 31%, from 0.39 to 0.51 mg O2 h?1 g?1, occurring 14 min after food intake. The carrying capacities for industrial cultures of G. maculatus, were estimated using an allometric equation (OC=0.2363 ×W 0.612 ), a water flow rate of 1 m3 h?1 and an input oxygen concentration of 10 mg L?1 at 12 °C. The density culture of whitebait (4 g) can be allowed to reach 8–11 kg m?3; therefore, these stocking densities reduce the risk of hypoxia and mortality, ensuring the appropriate growth and feed conversion rates.  相似文献   

16.
An energetic method was used to investigate the effects of stocking density and dissolved oxygen (DO) concentration on the growth of juvenile Paralichthys olivaceus. Fish, with initial weights of 14±2.1 g, were exposed to a normal and a high DO of 5.5±0.5 and 14±2 mg L?1, as well as four stocking densities per DO concentration (100, 200, 300 and 400 ind m?2 for the normal DO and 200, 400, 600 and 800 ind m?2 for the high DO). The feed efficiency (FEW) decreased significantly with increasing stocking density and increased significantly with increasing DO concentration. The maximum weight was achieved at 400 ind m?2 under a high DO depending on our rearing conditions. The stocking density and DO concentration change energy ingestion and its allocation for respiration, growth and excretion lost in nitrogen excretion, but do not affect the energy loss through faeces. The results of the body composition of fish indicated that the stocking density and DO concentration had no significant effects on the moisture, lipid content and gross energy, but are affected by the same. Energetic analysis demonstrated that high DO concentrations could alleviate the growth depression caused by high stocking densities, decrease energy loss in respiration and nitrogen excretion and increase the energy proportion allocated to growth.  相似文献   

17.

Gilthead sea bream (Sparus aurata) was raised in six individual recirculating aquaculture systems (RAS) whose biofilters’ performance was analyzed. Fish were fed with three different diets (a control diet, a fishmeal-based diet (FM), and a plant meal-based diet (VM)) and with three different feeding strategies (manual feeding to apparent satiation, automatic feeding with restricted ration, and auto-demand feeding). For every combination of diet and feeding strategy, the mean oxygen consumption, ammonia excretion, and ammonia removal rate were determined. Fish fed with the VM diet consumed the most oxygen (20.06?±?1.80 gO2 consumed kg?1 day?1). There were significant differences in ammonia excretion depending on the protein content and protein efficiency of the diet, as well as depending on feeding strategy, which in turn affected ammonia removal rates. Fish fed by auto-demand feeders led to the highest mean ammonia removal rate (0.10 gN-TAN removed m?2 biofiltration area day?1), while not leading to peaks of high ammonia concentration in water, which preserve fish welfare and growth.

  相似文献   

18.
Nitrogen excreted by aquatic animals mainly takes the form of metabolic wastes such as feces and ammonia, which is accumulated in the intensive aquaculture system and causes serious environmental contamination. So it is very important to determine the waste excretion characteristics of aquatic animals for the development of practical and nonpolluting land‐based aquaculture. Abalone has a unique feeding habit and feeding regime, different from those of finfish; abalone gnaw feed seaweed to produce feces and ammonia continuously. In this study, the rates of feces production and ammonia excretion of pacific abalone, Haliotis discus hannai, of three shell lengths (3, 5, and 7 cm) were investigated under three different temperature conditions (12, 16, and 20 C). All experiments were performed in triplicate in a semirecirculating aquaculture system. Feces were collected for 5 d, and ammonia concentrations (total ammonia nitrogen [TAN]) in the tank inlet and outlet were monitored every 4‐h interval for 24 h at the fourth day of the feces collection. The regressions for the weight‐specific feces production rate (g feces/kg abalone/d) and the weight‐specific TAN excretion rate (mg TAN/kg abalone/d) in relation to water temperature (T, C) and shell length (L, cm) were weight‐specific feces production rate = exp(1.575 ? 281.2/T2 – 0.142L), r2 = 0.9550, and weight‐specific TAN excretion rate = exp(5.052 ? 277.1/T2 ? 0.136L), r2 = 0.9598. Pacific abalone produced 108.3–111.7 g feces and excreted 3.83 g TAN/kg seaweed ingested (dry weight).  相似文献   

19.
The effects of the density and type of food on oxygen consumption and ingestion rate of larvae of the white shrimp Penaeus setiferus fed diatoms Chaetoceros ceratosporum, flagellates Tetraselmis chuii and Artemia franciscana nauplii were analysed. Diatoms, flagellates and Artemia nauplii were fed at five densities from 10 to 5 × 103 cells mL?1, 0 to 4 × 103 cells mL?1, and 0.1, 0.5, 1.0, 1.5 and 2 nauplii mL?1, respectively. In three experiments, two of three types of food were maintained constant at concentrations of 30-40 × 103 cells mL?1 (diatoms), 2 × 103 cells mL?1 (flagellates) and 1 Artemia nauplii mL?1. The oxygen consumption in three experiments increased with larval stage, reaching maximum values in Mill except at lower feed concentrations. A maximum ingestion peak in MI was recorded in larvae fed diatoms, whereas that peak was observed in Mil in larvae fed flagellates. The maximum ingestion rate of Artemia nauplii was observed in Mill. Feed concentrations that produced an optimum metabolic rate as a consequence of equilibrium between ingested food and larval stages were obtained with 20 and 30 × 103 cells mL?1 of C. ceratosporum, 2 and 3 × 103 cells mL?1 of T. chuii, and 1.0 Artemia nauplii mL?1. These concentrations would be the most suitable for producing P. setiferus postlarvae.  相似文献   

20.
This investigation examined the effects of live prey availability on growth and survival of Sepia officinalis. Two independent experiments, comprising two feed rations each, were performed, using adequate prey size. In the first experiment, cuttlefish hatchlings were fed live mysids, Paramysis nouvelli [(feed ratio I (fr I)], at 15% body weight per day (bw day?1) (fr I15) and 30% bw day?1 (fr I30). In the second experiment, juvenile cuttlefish were fed live Atlantic ditch shrimp, Paleomonetes varians (fr II), under the same experimental design. In both experiments, the final mean weight, feeding rate and instantaneous growth rate were higher when animals were fed feed ratio fr II15 and fr II30 (30% bw day?1). The results indicate that prey availability influenced weight gain, irrespective of the prey used, during the first 2 months of cuttlefish life cycle. This effect seems to be more noticeable when a certain limit of prey is achieved. For cuttlefish fed fr II, the optimal prey density is thought to be under 2.5 g prey L?1 (i.e. 14 shrimp L?1). Results indicate that cuttlefish can withstand prey densities up to 120 mysids L?1 for cuttlefish up to 3 weeks old and 19 shrimps L?1 for cuttlefish up to 6 weeks old.  相似文献   

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