共查询到19条相似文献，搜索用时 281 毫秒
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樟脑型樟树芽器官离体培养技术 总被引：1，自引：0，他引：1
以樟脑含量＞70％的樟脑型樟树优株的嫩枝为外植体,进行芽器官离体培养技术研究.结果表明：每年的1～4月外植体灭菌消毒接种,无菌活体获取率比较高;适宜樟脑型樟树组培的基本培养基：M2 （MS＋Ca （NO3）2.4H2O 300 mg/L）;芽诱导培养激素组合：6-BA4 mg/L＋NAA1.5 mg/L＋KT 0.5 mg/L;芽增殖培养激素组合：6-BA3.0 mg/L ＋NAA0.5 mg/L ＋KT0.2 mg/L;在培养基中添加L-半光氨酸10 mg/L抑制芽褐化效果良好. 相似文献
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金红茵芋离体快速繁殖技术的研究 总被引：1，自引：0，他引：1
为了建立完善的金红茵芋快速繁殖技术体系,以带腋芽茎段为外植体,对其进行了离体培养。结果表明:初代培养基以MS+0.2 mg/L ZT+0.04 mg/L IBA+5.0 mg/L GA3较为适宜,外植体诱导率最高达85%;最佳增殖培养基是MS+1.0 mg/L ZT+0.5 mg/L KT+0.1 mg/L IBA,培养30 d后,增殖率达3.6,试管苗高度达2.0 cm;生根培养基以1/2MS+1.0 mg/L IBA效果为最佳,生根率达90%;适时移栽入泥炭+蛭石+珍珠岩+有机肥(体积比为6∶2∶1∶1)的混合基质中,成活率在80%以上。 相似文献
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通过对湖南省资兴市某已闭库并实施生态修复的铅锌尾矿库及周边受污染土壤和植物的采样分析,以ICP—ASE电感耦合等离子体发射光谱法测定了杉木、杨树、栾树、泡桐、香樟和乌桕等6种木本植物中Pb、Zn、Cu和Cd等重金属元素的含量,并运用转运系数和富集系数分析了植物相应的富集能力与转运特征。结果表明:6种植物中,对Pb富集系数最大的为乌桕,达到0.029;对Zn富集系数最大的为泡桐,达到0.374;栾树对Pb的转运系数达到1.314;泡桐对于Zn的转运系数达到1.452。6种植物均能适应铅锌重度污染的生长环境,其中乌桕、泡桐、栾树富集和转运Pb、Zn能力突出,适宜作为铅锌矿区植物修复的主要木本树种。 相似文献
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Dr. Hasan Çanakçioglu 《Journal of pest science》1972,45(10):152-154
Zusammenfassung Die Aphiden in den türkischen Forsten (114 Arten in 7 Familien) und deren Wirtspflanzen sowie ihre ö konomische Bedeutung sind in diesem Artikel zusammengestellt. Die wichtigsten Arten sind.Pineus orientalis (Dreyf.),P. pini (Macq.) (Adelgidae);Cinara cedri Mime.,C. palaestinensis H. R. Lamb. (Lachidae);Eriosoma lanuginosum (Hart.) (Pemphigidae);Phyllaphis fagi (L.) (Callaphidae) undAphis craccivora Koch (Aphididae).Weitere in den türkischen Wäldern verbreitete Blattlausarten können orts- und zeitweise von Bedeutung sein.
Summary The aphids of Turkish forests (114 species in 7 families), their host plants and economic importance are summarized. More important species are:Pineus orientalis (Dreyf.),P. pini (Macq.) (Adelgidae);Cinara cedri Mim.,C. palaestinensis H. R. Lamb. (Lachnidae);Eriosoma lanuginosum (Hart.) (Pemphigidae);Phyllaphis fagi (L.) (Callaphididae) andAphis craccivora Koch (Aphididae).The other aphids which are recorded in Turkish forest, in some extent are important species.相似文献
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Four levels of vegetative competition were used to quantify the growth of loblolly and shortleaf pines (Pinus taeda L. and P. echinata Mill.) in naturally regenerated, even-aged stands on the Upper Coastal Plain of southeastern Arkansas, USA. Treatments included: (1) no competition control, (2) woody competition control, (3) herbaceous competition control, and (4) total control of nonpine vegetation. After pines became established from natural seeding, herbicides were used to control herbaceous plants for 4 consecutive years and woody plants for 5 consecutive years. Even though 89% of crop pines on untreated check plots were free-to-grow 11 years after establishment, crop pines on vegetation control plots were larger (P<0.001) in mean dbh, total height, and volume per tree. From age 5 through 11 years, crop pine diameter growth increased on woody control plots and decreased on herbaceous control plots because of hardwood competition in the latter treatment. At age 11, crop pine volume production averaged 207 m3/ha on total control plots, 158 m3/ha on herbaceous control plots, 130 m3/ha on woody control plots, and 102 m3/ha on untreated check plots. 相似文献
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The AhDREB1 gene, cloned from Atriplex hortensis L., was transferred into black locust （Robiniapseudoacacia L.） by an Agrobacterium-mediated transformation. The results suggest that stems of black locust sub-cultured in vitro for 20 d are suitable for genetic transformation. The optimum concentrations of kanamycin and cefotaxime were 30 and 150 mg.L-1, respectively. Important factors affecting the transformation efficiency were studied by means of a L9（3^4） orthogonal design. An effective system for genetic transformation in black locust was developed as follows： the stems were pre-cultured for 2 d, immersed in the Agrobacterium solution （OD600 = 0.7） with 10 mg·L^-1 acetosyringone for 21 min and then co-cultured for 2 d. The selection pressures, changing from low to high, could improve transformation efficiency. The transgenic plants were identified by a PCR method. The PCR results indicated that the AhDREB1 gene had been integrated into the genome of black locust and two lines of the transgenic plants were obtained. 相似文献
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Harchand R. Dagla Aarti Paliwal M. S. Rathore N. S. Shekhawat 《Journal of Sustainable Forestry》2013,32(3):283-293
Leptadenia pyrotechnica (Forsk.) Decne belongs to the family Asclepiadaceae. It is commonly known as Kheemp in India. L. pyrotechnica is an important component of an arid ecosystem and source of fiber, forage, and medicines. We report here in vitro propagation protocol of L. pyrotechnica. Cotyledonary nodes of in vitro raised seedlings were used as an explant for multiplication of shoots. Shoots were multiplied on Modified Murashige and Skoog's (MMS) medium containing 1.33 μM BAP and additives (50 mg L?1 ascorbic acid and 25 mg L?1 each of citric acid, arginine, and adenine). Cultures were maintained at 30 ± 2°C temperature, 50–60 μmol m?2 s?1 SFP, 12 hr day?1 photoperiod, and 60% relative humidity (RH). In vitro multiplied shoots were rooted in vitro on half-strength MS medium containing 2.46 μM IBA and 100 mg L?1 activated charcoal. Shoots were rooted ex vitro using 2460 μM IBA pretreatment for 15 min. Plantlets were hardened in a greenhouse, and survived on a mixture of sand, garden soil, and organic manure in 3:1:1 ratio in polybags in natural habitats. 相似文献
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A.V. Raghu S.P. Geetha Gerald Martin Indira Balachandran P.N. Ravindran 《Journal of Forest Research》2006,11(1):57-60
An efficient system was developed for direct plant regeneration from in vitro-derived leaf explants of Embelia ribes Burm. f., a vulnerable medicinal woody climber of the Western Ghats of India. The in vitro procedure involved three steps
that included induction of shoot initials from leaf tissue, regeneration and elongation of shoots from the shoot initials,
and rooting of shoots. The induction of shoot initials was achieved on Murashige and Skoog (MS) solid medium supplemented
with different concentrations of thidiazuron (TDZ). The best medium for shoot induction was MS with 0.272 μM TDZ. Numerous shoot primordia developed within 2–3 weeks on the leaf margin as well as on the midrib region, without any
callus phase. In the second step, the shoot clumps separated from the leaf explant on transfer to MS basal medium, resulting
in the differentiation of 90% of the shoot initials into well-developed shoots. The 2- to 3-cm-long shoots rooted on half-strength
MS basal medium supplemented with 4.90 μM indole-3-butyric acid (IBA) and 3% (w/v) sucrose in the third stage. The rooted plants could be established in soil with
70% success. This protocol could be utilized for in vitro propagation and conservation of this important threatened medicinal
plant. 相似文献
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Forage value of 10 deciduous woody fodder species during six stages of maturity as well as their acceptance by goats in July and August were studied in Macedonia, northern Greece. The fodder species studies wereAmorpha fruticosa L.,Carpinus orientalis Mill.,Colutea arborescens L.,Corylus avellana L.,Fraxinus ornus L.,Ostrya carpinifolia Scop.,Pyrus amygdaliformis Vill.,Quercus pubescens Willd.,Quercus sessiliflora Salich, andRobinia pseudoacacia L. with two accessions, common and spineless. All these species are integral components of the mediterranean silvo-pastoral systems. Monthly forage samples were collected throughout their growing period (May to November), which coincides with the long dry summer when herbaceous plants are dormant, and analyzed for crude protein content, neutral detergent fiber (NDF), lignin and in vitro organic matter digestibility (IVOMD). Comparing the leguminous species (Robinia pseudoacacia, Amorpha fruticosa, Colutea arborescens) with the remaining species, the leguminous one had on average a higher (P0.05) content of crude protein (21.1%, 21.0% and 17.1% vs. 10.5–13.3%, respectively) and higher (P0.05) IVOMD values (58.4%, 56.0% and 60.0% vs. 47.3–52.8, respectively). Moreover, the leguminous species were among species with lower NDF (42.3%, 45.0% and 35.2% vs. 36.0–48.9%, respectively) and lignin content (7.1%, 8.3% and 9.1% vs. 7.6–10.9%). All species showed a significant increase in crude protein when new leaves appeared (May). Most of the fodder species had digestibilities in the higher range (>55% IVOMD) and low NDF content (<53.5%) during the growing season (first five stages). Lignin contents ranged from 5.9% (A. fruticosa, May) to 16.0% (O. carpinifolia, Nov.).Robinia pseudoacacia had the highest relative acceptance index whileAmorpha fruticosa, Colutea arborescens andCorylus avellana the lowest. It is concluded that deciduous woody fodder species may play a significant role as sources of nutrients in the mediterranean silvo-pastoral systems during the summer period of seasonal nutritional shortage. 相似文献