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1.
Studies were conducted to investigate the possibility of embryo transfer in the cat during the non-breeding season. Estrus was induced in 19/22 (86.4%) cats using a porcine pituitary gland preparation. Uterine horns were flushed in 5 cats 6-8 days after mating with expanded blastocysts being collected from 4 cats. One to nineteen blastocysts per cat were transferred to the uterine horns of 6 recipient cats in which ovulation had been induced with HCG. The time differences between time of ovulation in donor and recipient animals were 0.5 days earlier in the recipient (2 cats), 1 day later in the recipient (3 cats), and no difference (1 cat); conception occurred in all the recipients. The ratio of fetuses to transplanted embryos were 1/1, 1/2, 2/3, 2/6, 4/7, and 2/19, respectively. Fetal death occurred in 2 cats at days 22 and 25 and abortion occurred in 3 cats at days 34, 35 and 39. There was a delay in the expulsion of placentae in the animals that experienced fetal death on days 22 and 25, expulsion occurring on days 36 and 56, respectively. One cat was treated with progesterone and carried 2 fetuses to day 66; pregnancy was terminated by cesarean section. In conclusion, it was demonstrated that embryo transfer can be performed in cats in which estrus and ovulation have been induced with porcine pituitary gland preparation during the non-breeding season. However, luteal activity needs to be supplemented by exogenous progesterone administration to maintain pregnancy.  相似文献   

2.
To obtain normal kits by embryo treansfer (ET) during the non-breeding season, maintenance of pregnancy was carried out by administration of sustained action progesterone (P4) in queens. Embryos were recovered six days after mating from five donor queens in which ovulation was induced by administration of eCG and hCG. The number of embryos recovered ranged from 24 to 53 (mean: 37.2 +/- 6.4) per animal and most embryos were compacted morulae. The yield of embryos was 49.0-93.3% (mean: 73.8 +/- 9.6%). As for recipients, porcine pituitary gland preparation and hCG were administered to 19 queens and estrus and ovulation were induced in 18 queens (94.7%). These queens underwent intrauterine ET of five compacted morulae and 17 cats (94.4%) were impregnated. The number of implantations was 2-5 (mean: 3.7 +/- 0.3). Among these impregnated queens, 15 cats received P4 adminstration starting on day 24 of gestation and 1-5 newborns (mean: 3.4 +/- 0.3) were obtained by normal delivery or caesarean section on day 64-69 of gestation. However, two animals that were not treated with P4 underwent spontaneous abortion about the mid gestational period. Therefore, it is possible to obtain normal kits from queens in the non-breeding season by ET with maintenance of pregnancy by P4 administration.  相似文献   

3.
The present study aimed to investigate the fertility of ewes artificially inseminated with three different methods using a synthetic semen extender, AndroMed. The three methods of artificial insemination (AI) were cervical AI with fresh-diluted or frozen-diluted semen at observed estrus, and an intrauterine AI with frozen-thawed semen. A total of 80 ewes were treated with a controlled internal drug release (CIDR) containing 0.3 g progesterone per device for 12 days. In Experiment 1 (26 Suffolk ewes), superovulation was induced with 20 mg follicle-stimulating hormone and 250 IU equine chorionic gonadotropin (eCG) two days and one day before CIDR removal, respectively, during the non-breeding season. In Experiment 2 (54 Suffolk and Suffolk crossbred ewes), an intramuscular injection of 500 IU eCG was administered one day before CIDR removal to synchronize estrus and ovulation during the breeding season. In Experiment 1, fresh-diluted or frozen-thawed semen was deposited into the cervical orifice after estrus detection, and an intrauterine AI with frozen-thawed semen was performed by laparoscopy at a fixed-time basis without estrus detection. Embryos were recovered by uterine flushing 6 days after AI, and the rates of recovered, fertilized (cleaved) ova and embryos at the morula or blastocyst stage were compared among the three AI methods. In Experiment 2, the pregnancy rates after the three AI methods were compared. In Experiment 1, the rates of recovered ova were not significantly different among the three AI methods (52.5-56.7%). The rate of fertilized ova (81.0%) by laparoscopic AI with frozen-thawed semen was significantly higher compared with cervical AI of fresh-diluted (25.5%) or frozen-thawed (3.5%) semen, but the rate of embryos at the morula or blastocyst stage (17.6%) was significantly lower than that of the cervical AI with fresh-diluted semen (69.2%). The rates of ewes yielding fertilized ova were not significantly different among the three groups (44.4, 11.1 and 62.5% for cervical AI with fresh-diluted and frozen-thawed semen and intrauterine AI with frozen-thawed semen). In Experiment 2, the pregnancy rate of ewes intrauterinally inseminated with frozen-thawed semen (72.2%) was significantly higher than those of ewes inseminated cervically with fresh-diluted (5.5%) or frozen-thawed (0.0%) semen. The present results showed that acceptable fertilization and pregnancy rates could be obtained by an intrauterine AI with frozen-thawed semen using a synthetic semen extender (AndroMed), but not sufficient by the cervical AI with either fresh or frozen semen.  相似文献   

4.
作者尝试在非繁殖季节应用外源促性腺激素对家猫进行人工诱导发情,并判断其较为准确的排卵时间。试验采用PMSG两次注射法对雌性家猫进行诱导发情,注射总剂量为150 IU,PMSG处理后的第6 d肌肉注射hCG（100 IU/只）并合笼配种。分别于合笼后的24、30、36 h手术法摘除家猫卵巢及输卵管部,观察记录卵巢排卵情况并对排卵侧的输卵管进行冲胚。试验结果显示,利用外源性促性腺激素可使家猫在非繁殖季节发情,且排卵时间范围为合笼配种后30 h左右。  相似文献   

5.
OBJECTIVE: To determine the effect of immunization with bovine luteinizing hormone receptor (LH-R) on ovarian function of cats. ANIMALS: 9 adult female domestic cats. PROCEDURE: 7 cats were immunized with 0.5 mg of LH-R encapsulated in a silastic subdermal implant (3 x 10 mm); 2 served as control cats. Receptors had 80% specific binding to 125I-human chorionic gonadotropin with a binding capacity of 2,682 pM/mg. Cats received booster injections of LH-R. Cats were induced to ovulate with luteinizing hormone (LH) releasing hormone on day 345. Samples of venous blood and vaginal cells were collected through day 395. Observation of estrus behavior continued until day 516. Serum concentrations of estradiol, progesterone, thyroid gland hormones, LH, and LH-R antibody were determined. RESULTS: LH-R antibody was detected in the sera of immunized cats within 21 days after implantation. Detection of LH-R antibody was associated with suppression of serum progesterone to < or = 0.5 ng/mL during the study period, compared with concentrations of 5 to 10 ng/mL in control cats. Immunized cats did not display signs of estrus. Release of LH after administration of LH-releasing hormone indicated an intact hypothalamic-pituitary axis but poor corpus luteum function. Serum estradiol concentrations remained between 30 to 40 pg/mL in immunized and control cats. With the decrease antibody titers, hormone concentrations returned to a pattern consistent with that during fertility. CONCLUSIONS AND CLINICAL RELEVANCE: Active immunization with LH-R suppressed corpus luteum function in cats. The effect was reversible. An LH-R-based antifertility vaccine may have clinical application in other vertebrates.  相似文献   

6.
Three cases of fibroepithelial hyperplasia (FEH) of the mammary gland in the cat are reported. A one year old female cat had a distinct enlargement of the middle mammary glands, one on each side, 5 days after the first estrus. One week later the cat was treated with medroxyprogesterone acetate (Depo-Promone). The affected glands, along with the remaining glands, increased further in size. A five year old female cat was treated with Proligeston (Covinan) for the suppression of estrus. Two weeks later fibroepithelial hyperplasia occurred in two glands, one with a well demarcated ulceration. A seven months old male cat was treated with delmadinon acetate (Tarden) because of urine spraying. Two months later he had enlargement of all mammary glands. All three cats were treated with the progesterone antagonist Aglépristone (Alizine). Within 5 to 11 weeks the mammary glands had regressed to normal.  相似文献   

7.
OBJECTIVES: To determine uptake of beta-carotene by ovarian and uterine tissues and influence of dietary beta-carotene on steroidogenesis and production of uterine protein during the estrous cycle in cats. ANIMALS: 56 female cats. PROCEDURE: Cats were fed diets containing 0, 0.4, 2, or 10 mg of beta-carotene daily for 8 weeks prior to detection of estrus. At time of observed estrus, all cats were manually induced to ovulate. Blood samples were obtained at estrus and every 2 days until day 14 after ovulation. On that day, cats underment laparotomy, and the ovaries and uterus were removed. Uterine contents were flushed, and luteal and endometrial tissues were obtained. RESULTS: Concentrations of beta-carotene in plasma and luteal and endometrial tissues increased in a dose-dependent manner. Concentrations of plasma progesterone were higher between days 6 and 10 after ovulation in cats fed diets containing beta-carotene and continued to increase through day 14 after ovulation in cats fed a diet containing 10 mg of beta-carotene. Plasma concentration of estradiol-17beta also was higher between days 0 and 4 after ovulation in cats fed diets containing beta-carotene. Cats fed a diet containing 10 mg of beta-carotene had the highest plasma estradiol concentration. Total uterine protein concentration was higher in cats fed beta-carotene, compared with values for cats fed an unsupplemented diet. CONCLUSION AND CLINICAL RELEVANCE: Cats readily absorb beta-carotene. Increased concentrations of progesterone, estradiol, and uterine protein may provide more optimal ovarian function or a better uterine environment for embryonic survival and development.  相似文献   

8.
Two experiments were conducted to compare the effect of estrus induction by controlled internal drug release (CIDR) and intravaginal cream containing 500 mg progesterone (P cream) in ewes during the non-breeding season. In the first experiment, twenty-four ewes were randomly grouped for two treatments with the different intravaginal devices for 12 days: Group A was the CIDR group and Group B was the P cream group. Blood was collected from all treated ewes, and progesterone (P(4)), estradiol 17-beta (E(2)) and luteinizing hormone (LH) concentrations were measured by enzyme immunoassay. In the second experiment, the conception rates from natural mating, estrus-detected AI (inseminated 12 h after estrus detection), or fixed-time AI (inseminated 42 h after removal of an intravaginal device) in 127 ewes treated with CIDR or P cream were compared. In Experiment 1, the rate of estrus induction and the time of estrus onset after device removal were 91.7% and 36.3 +/- 15.7 h in Group A, and 100% and 35.0 +/- 12.6 h in Group B, respectively. There were no significant differences between the devices. The mean plasma P(4) concentration in Group B was significantly (P < 0.01) lower than Group A between day -9 and day -1 (Day 0: the day of device removal). However, no significant differences were found in the mean E(2) concentrations of the two groups after treatment. The mean time of estrus onset in ewes with an observed LH surge and the time of LH surge after treatment were 23.3 +/- 8.7 h and 30.3 +/- 5.0 h for Group A and 27.6 +/- 6.5 and 26.3 +/- 8.0 h for Group B, respectively, and there were no significant differences. However, a significant difference (P < 0.05) was found in the mean time from the time of estrus onset to LH surge between Group A (6.4 +/- 6.7 h) and Group B (-1.3 +/- 4.1 h). In Experiment 2, the conception rates for natural mating, estrus-detected AI, and fixed-time AI were 55.0, 29.4, and 25.0% for Group A and 40.7, 25.0, and 42.1% for Group B, respectively, and there were no significant differences. These results suggest that the effect of induction of estrus and ovulation and the rate of conception after treatment were comparable to CIDR even though the plasma P(4) concentration of the P cream method tended to be low during the insertion period.  相似文献   

9.
The objective of this retrospective study was to estimate using magnetic resonance imaging the size range of the pituitary gland in cats who had no evidence of pituitary disease. The pituitary gland was measured from transverse and sagittal magnetic resonance postgadolinium T1-weighted images in 17 cats. The cats were 0.83 to 15 years of age and weighed between 2.9 and 6.5 kg. Linear pituitary measurements were performed on a dedicated workstation using electronic calipers. Mean (+/- standard deviation) pituitary gland length was 0.54 cm (+/- 0.06 cm) and mean width was 0.50 cm (+/- 0.08 cm). Mean pituitary gland height measured on sagittal images was 0.34 cm (+/- 0.05) and measured on transverse images was 0.32 cm (+/- 0.04 cm). Mean pituitary volume was 0.05 cm3 (+/- 0.01 cm3). There was no significant correlation between cat weight (kg) and pituitary volume or age and pituitary volume. The pituitary gland appearance varied on pre- and postcontrast T1-weighted images. On the precontrast images, the majority of pituitary glands had a mixed signal intensity. On postcontrast images, uniform pituitary gland enhancement was seen commonly.  相似文献   

10.
Effects of age of cow and a norgestomet (N) implant on number of embryos and endocrine responses to induction of superovulation were studied in old (greater than 12 yr) and young (5 to 7 yr) lactating beef cows. Seventeen cows (8 old and 9 young) received a 6-mg N ear implant on d 4 or 5 of the cycle (d 0 = estrus); the remaining 17 cows (9 old and 8 young) served as untreated controls (C). All animals were treated with 38 mg of porcine follicle-stimulating hormone (FSH-P) in decreasing dosages over a 4.5-d period beginning on d 10 or 11. Regression of the corpus luteum was induced with injections of PGF2 alpha at 0800 and 2000 on d 4 of FSH-P treatment; implants were removed at the second injection of PGF2 alpha. Cows were inseminated artificially 12 and 24 h after onset of estrus. Embryos were collected on d 7 or 8 postinsemination. Blood samples were obtained daily at 0800 from 2 d prior to initiation of treatment with FSH-P until collection of embryos. An additional sample was collected each day at 2000, from the first injection of PGF2 alpha to 1 d after onset of estrus. Samples were assayed for luteinizing hormone (LH), progesterone (P4), follicle stimulating hormone (FSH) and estradiol-17 beta (E2). Total number of embryos plus ova recovered was lower (P less than .01) in N-treated (5.2 +/- 1.1) than in C-treated (10.6 +/- 1.6) cows.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

11.
[目的]研究前列腺素F2α（PGF2α）、孕马血清促性腺激素（PMSG）、人绒毛膜促性腺激素（hCG）3种激素在不同处理方案下对内蒙古通辽地区乏情期母驴卵泡生长情况、排卵率、发情率、受胎率与妊娠率的影响。[方法]将50头2～6岁、体重接近、体况良好、处于乏情期的母驴随机分为A、B、C、D4组,其中D组为未使用任何激素的空白对照组。首先,采用PGF2α、PMSG进行诱导发情试验,对A、B、C3组母驴注射2mL／头PGF2α,连续处理5d,第6天对A、B组母驴分别注射PMSG 1000IU,头和500IU,头;C组不做PMSG处理。诱导发情后将A、B组再细分为A1、A2和B1、B2组,当A1、B1、C3组中母驴卵泡直径大于35mm时注射hCG（总量1500IU）进行诱导排卵;A2、B2不做hCG处理,由卵泡自然发育至排卵。对所有诱导发情后有发情表现的母驴进行自然本交。同时,在试验期间利用直肠检查和B超检查对母驴的卵巢发育进行监测。『结果]c组发情率为30％,经PGF2α处理第5天时卵巢黄体基本全部消退;A组和B组的发情率分别为93．33％和86．66％,二者差异不大,但均明显高于C组。A1、B1最终的排卵率为100％和77．78％,而A2和B2的排卵率为60．00％和50．00％。A,组受胎率和妊娠率最高,分别达到了88．89％和80．00％,其次是B,组分别为66．67％和60．00％,B2、C2组的受胎率和妊娠率都较低,D组虽然有1头母驴发情,但配种后未妊娠。[结论]单独使用PGF2α对非繁殖季节母驴诱导发情效果不明显,但其对消除母驴黄体有明显作用;PGF2α与PMSG联用对诱导乏情母驴发情效果较好。使用PGF2α与PMSG对处于乏情期的母驴诱导发情后,注射hCG对母驴卵巢上优势卵泡具有明显的促进排卵作用,能加快优势卵泡的排卵,且明显提高母驴的排卵率。该试验确定的最佳方案为：注射0．2mgPGF2α连续处理5d,第6天注射PMSG 500lU,当优势卵泡直径达35mm时注射hCG 1500lU,排卵后配种。  相似文献   

12.
In dogs, embryo transfer (ET) techniques such as induciton of excessive ovulation and synchronization of estrus have not progressed well. Therefore, using embryos at various developmental stages, ET was investigated in dogs from a beagle colony in which the ovulation days were close, as estimated by the progesterone level. Embryos were, recovered 8-11 days after ovulation (4-9 days after mating) by excising the oviducts and uteri (excision method) in 16 animals and by surgical flushing of the uteri at laparotomy (surgical method) in 3 animals. In 24 dogs with -4 to +2 days of difference in the timing of ovulation between donor and recipient dogs, 1-10 embryos at the 8-cell to blastocyst stages were transferred per animal. The mean embryo recovery rate by the excision method (97.1%) was significantly higher than that by the surgical method (42.5%) (p<0.01). Twelve (57.1%) of 21 animals with -1 to +2 days difference in ovulation day became pregnant after the transfer of 8-cell to blastocyst stage embryos. Although 3 dogs with -4 to -2 days of difference of ovulation day underwent ET of morula or compacted morula, none of these dogs became pregnant. The mean ratio of the number of newborns to the number of transferred embryos was only 51.9%. The mean duration of the period between ovulation and delivery in the pregnant recipients was 65.8 days, which tended to be longer than that in natural mating. These results demonstrate that pregnancy can be induced by ET at the 8-cell to blastocyst stage in dogs with -1 to +2 days difference in ovulation day.  相似文献   

13.
Nature of early reproductive failure caused by bovine viral diarrhea virus   总被引：2，自引：0，他引：2  
A 2-part study was undertaken to determine the effect of bovine viral diarrhea (BVD) virus on fertilization and early development of embryos. In experiment 1, 10 seronegative cows were superovulated and artificially inseminated twice during estrus. After the second insemination, 5 of the cows received intrauterine infusion of BVD virus suspension. The other 5 cows received suspending medium only and served as controls. All 10 cows were slaughtered on day 3, and ova and embryos were collected for morphologic evaluation. A total of 49 and 52 ova and embryos were collected from the control and virus-treated cows, respectively. Among the ova and embryos collected from control cows, 81.6% were fertilized, whereas only 52% were fertilized in the virus-treated group. The statistically significant difference (P less than 0.01) indicated that the virus interferes with fertilization. In experiment 2, the protocol was identical except for slaughter on day 13. Seventy-nine ova and embryos were collected from the 6 control cows, and the 6 virus-treated cows yielded 59 ova and embryos. Of the total ova and embryos recovered on day 13, 88.6% and 50.8% were hatched and developing normally in the control and virus-treated groups, respectively. The difference was highly significant (P less than 0.001). Unfertilized ova and degenerating embryos could not be differentiated on the basis of morphologic appearance. The nearly identical percentages of unfertilized ova in experiment 1 and unhatched ova and embryos in experiment 2 strongly suggested that fertilization failure is the principal manifestation of the observed adverse effect of BVD virus infection.  相似文献   

14.
Treatment with a progesterone-releasing intravaginal device between days 5 and 12 after estrus elevated (p less than 0.05) plasma progesterone concentrations between days 6 and 8 in comparison with controls. Treatment with injectable progesterone (200 mg) on days 5, 7, 9 and 11 postestrus did not increase plasma progesterone concentrations over controls. The administration of 1500 IU human chorionic gonadotrophin (hCG) on day 5 after estrus resulted in a sustained increase (p less than 0.01) in plasma progesterone concentrations from day 8 until day 20 when measurements ceased. Pregnancy rates, as a result of artificial insemination (AI) at the pretreatment estrus, in these treatments (n = 12-14 each), were unaffected by any of the treatments and ranged from 57.1 to 75.0% at 45-60 days post-AI. In a field trial, of 36 repeat breeder cows treated with 1500 IU hCG 5.5 days after insemination, 47.2% were pregnant at 60 days, whereas 39.5% of saline-treated controls were diagnosed pregnant. Treatment with hCG significantly (p less than 0.05) increased milk progesterone concentrations over controls on days 14 and 20 after insemination.  相似文献   

15.
To evaluate the ability of the CatanDog's tag to eliminate fleas, inhibit egg production and prevent flea infestations, six domestic shorthaired cats were randomly allocated to two treatment groups and housed individually in stainless steel metabolic cages. Three cats were each fitted with a CatanDog's tag; the other three cats were not fitted with tags and served as controls. Following a 42-day acclimation period, each of the six cats was infested with 100, 1-3 day post-emergence, adult Ctenocephalides felis felis (Bouché) on days 0, 7, 14, 21, and 27. Flea egg production was determined by collecting and enumerating eggs 2, 4 and 6 days after each infestation. Viability of eggs was determined by placing 100 eggs recovered from each cat in rearing media in an insect rearing chamber and determining adult emergence at 28 days. Adult fleas were recovered from cats 6 days post-infestation by thoroughly combing each cat to remove fleas. To determine if the tags provided protection from infestation, the six cats were placed into a 8.53mx4.36 m room with 400 cat fleas for 3h. Cats were then combed to remove and enumerate fleas. The CatanDog's tags had no significant effect upon egg production, egg viability, or adult fleas infesting cats. In addition there was no difference in the numbers of fleas recovered from the cats placed in the flea-infested room.  相似文献   

16.
Thirty nonlactating Holstein cows were superovulated to determine the effect of artificial insemination time on fertilization status and embryo quality. During the luteal phase of the estrous cycle, cows were administered 38 mg FSH-P in a 4-d descending dose regimen. Luteolysis was induced with two injections of prostaglandin on the last day of FSH-P treatment. All cows were continuously monitored for behavioral estrus using the HeatWatch estrus detection system. All cows were inseminated once with one .5-mL straw (50 x 10(6) sperm) at either 0 (n = 10), 12 (n = 10), or 24 h (n = 10) after the first standing event. The elapsed time (mean +/- SD) from the first prostaglandin dose to the first standing event was 39.4 h +/- 7.7 h. The (mean +/- SD) duration of behavioral estrus was 13.2 h +/- 4.1 h. The (mean +/- SD) number of standing events was 27 +/- 17. Five hundred twenty-nine embryos and ova were recovered nonsurgically 6 d after insemination. Fertilization rates were 29 (0 h), 60 (12 h), and 81% (24 h) (P < .01). Percentages of excellent and good, fair and poor, and degenerate embryos were not different (P > .05). Percentages of embryos with accessory sperm were 5 (0 h), 8 (12 h), and 41 (24 h) and differed between the 0 and 24 h and the 12 and 24 h inseminations (P < .01). Artificial insemination of superovulated, nonlactating Holstein cattle 24 h after onset of estrus increased fertilization rate and percentage of embryos with accessory sperm compared with insemination at 0 or 12 h after onset of estrus. Embryo quality was not affected by time of insemination.  相似文献   

17.
Hyperadrenocorticism in a cat   总被引：2，自引：0，他引：2  
A diabetic cat with hyperadrenocorticism had polydipsia, polyuria, ventral abdominal alopecia, thin dry skin, and a pendulous abdomen. Results of laboratory testing indicated persistent resting hypercortisolemia, hyperresponsiveness of the adrenal glands (increased cortisol concentration) to ACTH gel, and no suppression of cortisol concentrations after administration of dexamethasone at 0.01 or 1.0 mg/kg of body weight. Necropsy revealed a pituitary gland tumor, bilateral adrenal hyperplasia, hepatic neoplasia, and demodicosis. Adrenal gland function was concurrently assessed in 2 cats with diabetes mellitus. One cat had resting hypercortisolemia, and both had hyperresponsiveness to ACTH gel (increased cortisol concentration) at one hour. After administration of dexamethasone (0.01 and 1.0 mg/kg), the diabetic cats appeared to have normal suppression of cortisol concentrations. The effects of mitotane were investigated in 4 clinically normal cats. Adrenocortical suppression of cortisol production occurred in 2 of 4 cats after dosages of 25, 37, and 50 mg/kg. Three cats remained clinically normal throughout the study. One cat experienced vomiting, diarrhea, and anorexia.  相似文献   

18.
Progesterone receptors in feline mammary adenocarcinomas   总被引：1，自引：0，他引：1  
Estradiol and progesterone receptors were measured in tumor cytosols from 3 intact and 4 neutered female cats with spontaneously occurring mammary adenocarcinomas. Serum from 2 of the intact cats which had been in estrus 4 and 4 to 6 weeks before tumor excision contained progesterone concentrations of 16.2 and 2.2 ng/ml, respectively; serum progesterone in the other cats was less than 2 ng/ml. Estradiol receptors were not detected in any cytosols. Progesterone receptors were detected in all of the cytosols, in concentrations ranging from 4.0 to 11.7 (mean = 7.2) fmol/mg of protein. Scatchard plot analysis of tumor cytosol from an 8th cat with mammary adenocarcinoma revealed presence of high affinity progesterone binding with a dissociation constant (Kd) of 3.47 nM. Tumor receptor content could not be correlated with stage of the estrous cycle nor with whether the cat was intact or neutered.  相似文献   

19.
Hyperadrenocorticism in cats: seven cases (1978-1987)   总被引：1，自引：0，他引：1  
Hyperadrenocorticism was diagnosed in 7 cats with concurrent diabetes mellitus. Four cats had pituitary adenoma with bilateral adrenocortical hyperplasia, 1 cat had pituitary carcinoma with bilateral adrenocortical hyperplasia, 1 cat had adrenocortical carcinoma, and 1 cat had adrenocortical adenoma of the left adrenal gland. One year later, adrenocortical adenoma involving the right adrenal gland also was diagnosed in this cat. Clinical signs included polyuria and polydipsia (n = 7), development of pot-bellied appearance (n = 5), dermatologic alterations (n = 5), lethargy (n = 3), weight loss (n = 3), dyspnea/panting (n = 2), and recurrent bacterial infections (n = 2). In 6 cats, the diagnosis of hyperadrenocorticism was established before death on the basis of results of the ACTH stimulation test (n = 3) and the dexamethasone screening test (n = 5). Pituitary-dependent hyperadrenocorticism was differentiated from adrenocortical neoplasia on the basis of results of the dexamethasone suppression test (n = 4), endogenous ACTH concentration (n = 3), results of abdominal radiography and ultrasonography (n = 3), and exploratory celiotomy (n = 1). Four cats died or were euthanatized without treatment attempts. Treatment with mitotane followed by 60Co teletherapy was ineffective in one cat with pituitary adenoma. One cat with pituitary carcinoma died one week after bilateral adrenalectomy. Bilateral adrenocortical adenomas were removed surgically in the affected cat.  相似文献   

20.
In Exp. 1, 21 first-service cattle and seven repeat-breeder cattle, averaging 4.7 infertile services, were brought into estrus and superovulated by treatment with follicle-stimulating hormone and prostaglandin F2 alpha. At insemination, semen was deposited in the greater curvature of one uterine horn, about midway between the utero-cervical junction and the utero-tubal junction. Cattle were necropsied 2 to 7 d after estrus and ova were recovered and examined. The fertilization rate for first-service cows was 74% of 362 intact ova and for repeat-breeders, 43% of 128 intact ova (P less than .001). Fertilization rate in first-service cows was 81% on the side of semen deposition and 68% on the opposite side (P less than .01); the rates in repeat-breeders were 54% and 32% (P less than .025). Differences between sides were due mostly to four cows that averaged 93% fertilization on the side of semen deposition and 19% on the opposite side. The proportion of fertilized ova with accessory sperm (17%) did not differ between sides of the reproductive tract. In Exp. 2, 60 first-service and 32 repeat-breeder cows in natural estrus had semen deposited in the uterine body or in the greater curvature of one uterine horn, either on the side of impending ovulation or on the opposite side. At necropsy, 55 ova were recovered from first-service cows, of which 42 (76%) were intact and 13 (24%) were ruptured or fragmented. Of the 42 intact ova, 41 (98%) were cleaved. From the 32 repeat-breeders, 30 ova were recovered, of which 26 (87%) were intact and 4 (13%) were ruptured; 23 of the 26 intact ova (88%) were cleaved. Site of semen deposition had no significant effect on either fertilization rate or number of accessory sperm in either type of cow. First-service cows averaged more accessory sperm (40) than did repeat-breeders (19, P less than .01). Overall results indicated that sperm deposited deep in one uterine horn fertilized ova nearly as frequently in the opposite oviduct as in the adjacent oviduct except in 14% of superovulating cattle.  相似文献   

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