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1.
1. Glucose transporter (GLUT) proteins, one of which is the major insulin-responsive transporter GLUT4, play a crucial role in cellular glucose uptake and glucose homeostasis in mammals. The aim of this study was to identify the extent of mRNA expression of GLUT1, GLUT2, GLUT3 and GLUT8 in chickens intrinsically lacking GLUT4. 2. GLUT1 mRNA was detected in most tissues of 3-week-old broiler chickens, with the highest expression measured in brain and adipose tissue. GLUT2 was expressed only in the liver and kidney. GLUT3 was highly expressed in the brain. GLUT8 was expressed ubiquitously, with expression in kidney and adipose tissue relatively higher than that of other tissues. 3. Expression levels of GLUT isoforms 1, 3 and 8 in skeletal muscle tissue were very low compared to the other tissues tested. 4. [3H]Cytochalasin B binding assays on tissue from 3-week-old chickens showed that the number of cytochalasin B binding sites in skeletal muscle plasma membranes was higher than in liver plasma membranes. These results suggest that GLUT proteins and/or GLUT-like proteins that bind cytochalasin B are expressed in chicken skeletal muscles. 5. It is proposed that GLUT expression and glucose transport in chicken tissues are regulated in a manner different from that in mammals.  相似文献   

2.
选用临床检查健康的(26±2)日龄"杜×长×大"三元杂交断奶仔猪100头,按体质量和性别随机分为5组,每组20头,分别饲喂基础日粮、基础日粮+2000mg/kg氧化锌、基础日粮+3000mg/kg氧化锌、基础日粮+250mg/kg蛋氨酸锌、基础日粮+500mg/kg蛋氨酸锌。试验期14d。于断奶后0,7,14d,经前腔静脉采血,用原子吸收光谱仪检测血清中铜、铁、锌水平。试验结束时,每组选5头仔猪放血致死,取心、肝、肾、脑、脾、胸腺组织,测定铜、铁、锌含量。结果显示,仔猪断奶后,血清中铜、锌水平显著或极显著下降(P0.05或P0.01);添加不同锌源和锌水平的高锌日粮能提高断奶仔猪肝、肾、脑、血清锌的含量,显著或极显著降低仔猪血清铜和心、胸腺铜以及脾铁水平(P0.05或P0.01),对血清铁及其他组织铁含量无明显影响。这表明高锌日粮能增加断奶应激仔猪体内锌水平,降低部分组织中铜、铁含量。  相似文献   

3.
P Rudas  G Sályi  J Szabó 《Avian diseases》1986,30(2):293-297
The effect of malabsorption syndrome (stunting or runting syndrome) on the thyroid function of broilers was investigated in control and inoculated broilers from 1 to 29 days of age. The broilers were infected at 1 day of age with intestinal homogenates from chickens naturally suffering from this syndrome. The body weight of inoculated broilers was significantly (P less than 0.05) lower 1 week after inoculation than that of controls. The level of thyroxine in the serum of inoculated birds was lower (P less than 0.05) from day 6 through the remainder of the trial. The level of triiodothyronine of inoculated birds was depressed (P less than 0.05) on day 4, but 1 week later it returned to normal. The earliest phenomenon indicative of disturbance of thyroid function was the significant depression of 5'-deiodination in liver homogenates of inoculated broilers as early as day 2. It is concluded that thyroid function is one of the earliest targets of this syndrome.  相似文献   

4.
This study compares in vitro effects of propylthiouracil (PTU) and ronnel on the conversion of thyroxine (T4) to triiodothyronine (T3) in liver and kidney from Angus steers. Tissues were homogenized and incubated with T4 (1.3 microM) in the presence of 0 to 59 microM PTU or 0 to 49.7 microM ronnel. The T3 generated during a 30-min incubation was measured by radioimmunoassay. It was found that 1.47, 5.9 and 59 microM PTU decreased T4 to T3 conversion in liver and kidney by 62 and 88, 71 and 100, and 81 and 100%, respectively. The inhibition caused by 1.47 and 5.9 microM PTU was overcome by addition of 2 mM dithiothreitol (DTT). Ronnel in concentrations of 1.24, 6.22, 49.7 microM decreased T4 to T3 conversion in liver and kidney 46 and 45, 51 and 72, and 78 and 95%, respectively. However, with ronnel, the addition of DTT caused further inhibition. A Lineweaver-Burk plot of the data obtained using .32 to 6.43 microM T4 with 1.47 and 5.9 microM PTU or 6.22 and 12.44 microM ronnel indicated that PTU is an uncompetitive inhibitor (Ki = 1.67 microM) and ronnel is a noncompetitive (Ki = 15.5 microM) inhibitor of T4-5'-monodeiodination. The data suggest that decreased conversion of T4 to T3 by PTU or ronnel may be responsible for the increased plasma concentrations of T4 and slightly decreased plasma concentrations of T3 reported in steers treated with levels of both PTU and ronnel that are associated with growth stimulation.  相似文献   

5.
为了解细胞因子IL-4和IL-5在戊型肝炎病毒(HEV)感染中的表达及其作用,以新西兰大白兔为模型,腹腔注射感染HEV-4,攻毒后第7、14、21、28、35天对兔的心、肝、脑、肾等进行组织病理学观察,应用实时荧光定量PCR对肝脏、脑等器官中IL-4和IL-5的表达水平及变化进行检测.结果显示,肝脏中IL-4和IL-5...  相似文献   

6.
Objectives were to examine mechanisms underlying anabolic actions of cimaterol in skeletal muscle and to evaluate cimaterol's actions in hypothyroid and hyperthyroid rats. In the first study growing rats were fed either a control diet or a diet containing cimaterol for 10 days. In a second study sham-thyroidectomized and thyroidectomized (Tx) rats were assigned to one of 5 treatments: control (sham-Tx), Tx, Tx supplemented with cimaterol, Tx injected with triiodothyronine (T3), and Tx rats injected with T3 and supplemented with cimaterol. Effects of treatments on growth, muscle weights and urinary NT-methylhistidine (NMH) excretion were evaluated in both trials. Muscle was also collected for determinations of DNA, RNA, protein and activities of several proteolytic enzymes. Cimaterol caused muscle hypertrophy and increased urinary NMH excretion. Hence, anabolic actions of cimaterol may result from an increase in myofibrillar protein synthesis which exceeds changes in myofibrillar protein degradation. Urinary NMH excretion was reduced by thyroidectomy and increased in hyperthyroid rats and both hypothyroidism and hyperthyroidism were characterized by myopathy. Cimaterol increased muscle weights in hypothyroid but not in hyperthyroid rats. Therefore, cimaterol's anabolic properties are thyroid hormone-independent and antagonized by excess thyroid hormone. Anabolic actions of cimaterol in hypothyroid rat muscle were attributed to an action on protein synthesis because urinary NMH excretion was not affected by cimaterol but muscle RNA concentration was increased. Activities of cathepsins B, D and L and neutral proteinase were dose-related to thyroid status, however, were unrelated to cimaterol-dependent perturbations in NMH excretion. Control of muscle protein balance by thyroid hormones may involve regulation of these enzymes; however, control of muscle protein degradation by cimaterol is likely directed towards other proteolytic mechanisms or to mechanisms which alter susceptibility of myofibrillar proteins to degradation.  相似文献   

7.
Toxocara canis is a nematode of the Ascaridae family that normally parasites the small intestine of canid species. Humans are accidentally infected upon ingestion of embryonated eggs, and can manifest several clinical alterations such as fever, hepatomegaly, splenomegaly, respiratory symptoms, muscle pain and anorexia. In the present work, we investigated the kinetics of tissue distribution of L2 larva in lungs, liver, kidney, brain, skeletal muscle and myocardium. Also, we analyzed the blood and bronchoalveolar lavage fluid (BAL) for levels of IL-6, IFN-gamma, eotaxin and Regulated on Activation Normal T Cell Expressed and Secreted (RANTES) in experimental murine T. canis infection. We observed liver, lung and kidney lesions correlated to larva migration as early as the first day of infection. After the seventh post-infection day, larva could also be detected in brain, skeletal muscle and heart, as an indicator of biphasic migration pattern. Increased inflammatory activity was detected in BAL and plasma of infected animals, as was an intense eosinophil migration associated with an increase in the levels of all the cytokines studied. In conclusion, our results establish a tight correlation between tissue lesions caused by larva migration and increased plasma levels of pro-inflammatory and eosinophil chemotactic cytokines. Thus, murine T. canis infection may prove to be useful in understanding the role of cytokines in infection.  相似文献   

8.
In domestic animal medicine, changes in serum enzyme levels are routinely used as diagnostic tools to detect liver disease. Hepatic disease occurs in pinnipeds, but limited data are available on the tissue distribution of serum enzymes in marine mammals. The objectives of this study were to determine the tissue distribution of seven serum enzymes in three pinniped species. Enzymes evaluated were alanine aminotransferase (ALT), aspartate aminotransferase (AST), sorbitol dehydrogenase (SDH), lactate dehydrogenase (LDH), creatine kinase (CK), alkaline phosphatase (ALP), and gamma-glutamyl transferase (GGT) in tissues from California sea lions (Zalophus californianus) (n = 5), harbor seals (Phoca vitulina) (n = 5), and northern elephant seals (Mirounga angustirostris) (n = 5) that stranded and then died at a rehabilitation center. Samples were evaluated in duplicate from liver, skeletal muscle, cardiac muscle, kidney, adrenal, spleen, pancreas, lung, lymph node, and intestine. Patterns of tissue enzyme distribution were similar in all species, with SDH activity highest in liver and kidney, CK activity highest in skeletal and cardiac muscle, ALP activity highest in adrenal, and GGT activity highest in the kidney. Aspartate aminotransferase and LDH activities were less specific, with high activity in multiple tissues. Tissue ALT activity was high in the liver of all species, but was also high in cardiac muscle (California sea lions), skeletal muscle (harbor seals), and kidney (elephant seals). These results suggest that concurrent analysis of SDH, ALT, and CK would provide high specificity and sensitivity for the detection of hepatic lesions, and allow differentiation of liver from skeletal muscle lesions in pinniped species.  相似文献   

9.
The concentrations of thyroxine (T4), 3,3',5-triiodothyronine (T3) and 3,3',5'-triiodothyronine (reverse T3; rT3) in thyroid gland tissue and serum of the fetal pig (n = 68) from day 39 to 113 of gestation were measured. Tracer quantities of iodothyronines, displaying the onset of thyroid hormone activity, were found in the thyroid tissue on day 39, i.e. before the appearance of a measurable quantity of iodothyronines in the serum. The T4 and T3 thyroidal content showed the first rise between days 56 and 76. Then, T3 was increasing sharply from day 92 till birth, while T4 content was decreasing from about day 76 to a low value between day 92 and 105, and then showing an increase shortly before birth. The rT3 content was the highest on day 39 and then it was steadily decreasing to reach a nadir on about day 76. Measurable amounts of thyroid hormones (TH) in the serum were observed not earlier than on day 46 of gestation. Near birth, the tissues of the pig fetus are in a milieu characterised by the highest blood TH concentrations. The serum to thyroid concentration ratio for rT3 and T4 was generally below 1.0 until the last trimester of gestation, when it was over 5.0 for rT3 and over 4.0 for T4. By contrast, the T3 serum to thyroid ratio was below 0.5 throughout the gestation. The results show that the fetal pig thyroid displays a low rT3 and T4 content, but the marked T3 elevation observed near term supports the view that a high production and secretion of T3 near term may be a critical factor for normal postnatal adaptation to extrauterine cooling in the pig.  相似文献   

10.
Total serum alkaline phosphatase (ALP) activity is the product of the combined activity of isoenzymes from a number of tissue sources. In this study, a commercially available kit for electrophoretic separation of ALP isoenzymes in an agarose gel was used to separate ALP isoenzymes in feline tissue extracts and serum. Five separate bands of ALP activity were identified. These bands were numbered 1 to 5 with band 1 having the most anodal migration. The tissue of origin corresponding to the migration position of the isoenzymes are as follows: Band 3 was the liver isoenzyme, band 4 was the bone isoenzyme and ALP isoenzymes of both intestine and kidney migrate in the position labelled band 5. Band 1 appears to be related to albumin and does not represent true ALP activity. The tissue source of band 2 (a and b) was not identified. Serum ALP activity of mature, healthy cats is primarily of liver origin. Immature cats (< 1 year of age) have a greater proportion of the bone isoenzyme in the serum.  相似文献   

11.
Cisplatin is a chemotherapeutic agent widely used in treatment of several cancers. It is documented as a major cause of clinical nephrotoxicity and hepatotoxicity. The purpose of this study was to investigate the involvement of oxidative stress in the pathogenesis of cisplatin-induced liver and kidney injury. Wistar rats were divided into four groups. Group 1 (control) was intraperitoneally (IP) injected with a single dose of 0.85% normal saline. Groups 2, 3 and 4 were IP injected with single doses of cisplatin at 10, 25 and 50 mg/kg body weight (BW), respectively. At 24, 48, 72, 96 and 120 h after injection, BW, levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), blood urea nitrogen (BUN), creatinine, malondialdehyde (MDA), and activity of superoxide dismutase (SOD) and histology of the liver and kidney were evaluated. Cisplatin caused a reduction in BW of rats in groups 2, 3 and 4 at all post injection intervals. The levels of serum ALT, AST, BUN and creatinine and MDA of the kidney and liver were markedly increased especially at 48 and 72 h, whereas the activity of SOD was decreased after cisplatin injection. Liver sections revealed moderate to severe congestion with dilation of the hepatic artery, portal vein and bile duct and disorganization of hepatic cords at 50 mg/kg of cisplatin. Kidney sections illustrated mild to moderate tubular necrosis at 25 and 50 mg/kg of cisplatin. Therefore, oxidative stress was implicated in the pathogenesis of liver and kidney injury causing biochemical and histological alterations.  相似文献   

12.
Fan CK  Lin YH  Du WY  Su KE 《Veterinary parasitology》2003,113(2):145-155
Infectivity and pathogenicity to mice of embryonated eggs of Toxocara canis, that had been maintained in 2% formalin for 14 months at 4 degrees C, were evaluated by immunological and pathological assessment at 1, 4, 8, 12, 16, 20, 24, 28, 42 and 67 weeks post-infection (WPI). On each date, three infected mice and two age-matched uninfected mice were sacrificed for serum collection and histological processing of the liver, lungs, musculature, and brain. Infectivity assessment by enzyme-linked immunosorbent assay (ELISA) revealed that the overall immunological pattern of infected mice tended to be towards the Th2 type response. Serum IgG1 antibody titers in infected mice were significantly higher than that of the uninfected control mice throughout the trial (P<0.05). On the other hand, no significant difference in titers of IgG3 antibody, an indicator for the Th1 type response, was observed between the infected and control mice, except at eight WPI (P<0.05). Pathogenicity was assessed semiquantitatively by comparing the mean number or diameter of inflammatory foci as well as histopathological changes in the liver, musculature, brain, or lungs of the infected mice and the control mice. Each hematoxylin and eosin (H&E) stained tissue section slide was examined under 100x magnification and 15 random fields were counted. Degree of inflammatory injury among the four organs was scored and categorized into four levels: normal (0), mild (1+), moderate (2+), and severe (3+). An index of inflammatory injury (mean score of experimental group/mean score of 10 control groups of 20 uninfected mice) of 2-3 is considered as moderate to severe, 1-2 as mild to moderate, and 0-1 as normal to mild. Histopathological changes were moderate to severe in the liver and lungs, mild to moderate in the musculature, and only normal to mild in the brain throughout the trial. It is noteworthy that apocrine-like change in epithelial cells of the bile duct was observed in most of the infected mice from eight WPI onward. Furthermore, larvae trapped by organized granulomas were found in soft tissue near the musculature at 12, 20, and 28 WPI. Altogether, not only were the infectivity and pathogenicity of the 14-month-cultured T. canis embryonated eggs retained, the hatched larvae were also capable of eliciting some special pathological changes in the murine host.  相似文献   

13.
We evaluated changes in hematology and chemical profile, and the tissue retention of hematoporphyrin derivative (HpD) following the intravenous injection in dogs. HpD at concentrations of 1, 5, 10, and 15 mg/kg was intravenously injected to 16 dogs (n=4 each) and complete blood count (CBC) and blood chemistry were performed on days 1, 3, 5, and 7 after the injection. To examine tissue retention, HpD (5 mg/kg) was administered to 15 dogs and 3 dogs were euthanized on days 1, 2, 7, 14, and 28 after the injection, respectively, to collect the skin, muscle, small intestine, spleen, kidney and liver as tissue samples. There were no significant changes in CBC and blood chemical profile except for an increase in LDH concentrations in dogs given 10 and 15 mg/kg of HpD at day 3. The levels of HpD retention in the tissues were ranked in the following order: liver > kidney > spleen > intestine > muscle > skin.  相似文献   

14.
Weanling pigs were fed a 30% crude protein (CP) diet for 18 d and then assigned to one of three regimens for either 4 or 8 d: 1) fasting, 2) 3% CP, i.e., maintenance or 3) 30% CP after which they were bled, then sacrificed for tissue assessment. Relative to pigs fasted or fed 30% CP, feeding 3% CP resulted in decreased urea-N and NH3-N excretion in the urine. Arginase and ornithine transcarbamoylase (OTC) activities in liver, and arginase activity in kidney cortex were markedly lower in pigs fed 3% CP compared with those either fasted or fed 30% CP. Hepatic arginase and OTC, however, were higher in fasted pigs than in those fed 30% CP. Pigs fed 3% CP had much lower levels of free threonine, tyrosine, cystathionine, taurine and branched-chain amino acids in plasma, liver, kidney, muscle and brain than fasted pigs or those fed 30% CP. Threonine concentration in brain, liver, muscle and plasma increased as length of the fast increased. Fasted pigs had decreased free alanine levels in plasma, and decreased free serine levels in plasma and liver when compared with fed pigs. Inter-organ comparisons provided evidence that both alanine and serine were important gluconeogenic amino acids during fasting. In general, free amino acid levels in brain were similar between fasted pigs and those fed 30% CP. Fasting for 8 d caused a 10-fold elevation in urinary taurine excretion relative to that observed for 4-d fasted pigs.  相似文献   

15.
Oxytetracycline pharmacokinetics and tissue distribution were studied in rainbow trout following bolus i.v. administration at 5 mg/kg. The mean serum (log) drug concentration data were plotted against time (linear). The decay curve was described by a three-component exponential decay function and a three-compartment model. The t1/2 of rapid distribution was 0.9 h, the t1/2 of the slow distribution was 5.9 h and the t1/2 elimination was 81.5 h. Clearance was 25.4 ml/kg/h and Vd(area) 2988 ml/kg. Regression analysis of the serum levels for the three intervals, 0.5-2.0 h, 6.0-18.0 h, and 24-96 h, indicated that the rates of decay for each interval were 0.6151 h-1, 0.0564 h-1 and 0.0088 h-1 respectively. Rates of equilibration between tissues and serum were determined. Kidney equilibrated the fastest with t1/2 to equilibration of 1.1 h for H (anterior) kidney and 1.98 h for P (posterior) kidney. The highest drug levels were found in the liver and the lowest were in the brain.  相似文献   

16.
In the horse, 5'-nucleotidase (5'-NT) activity is found mainly in homogenates of lung, kidney, small intestine, mammary gland, liver and pancreas. Lower activities are present in brain and muscle. Activity can be demonstrated histochemically in the glomeruli and tubules of the kidney, in the sinusoidal borders of the hepatocytes and the bile duct epithelium as well as in the blood vessels of all organs. There is no significant difference between the 5'-NT activity in serum and plasma of normal horses and of horses suffering from a range of orthopaedic conditions. Previous findings that gamma glutamyl transferase activity is greatest in kidney and high in liver and pancreas have been confirmed.  相似文献   

17.
In 7 normal healthy Egyptian one-humped camels aged 3-4 years, the relationships were studied between enzyme activities of lactic dehydrogenase (LDH), creatine phosphokinase (CPK), alkaline phosphatase (ALP), acid phosphatase (ACP), and cholonesterase (CHE) of serum and organs as well as between ACP and ALP and between LDH and CPK. Liver, heart, kidney, and spleen tissue samples as well as serum were analysed for total enzyme activity. The following results were obtained: --The heart was the organ with the highest activities of ALP, LDH, and CPK, and it contained high values of CHE, whereas the lowest activities of all enzymes were recorded from serum. The spleen exhibited of the highest activity of ACP. --Each of the serum enzymes ALP, LDH, and CHE were in strong inverse relationship with the corresponding enzymes in the liver. Strong inverse relationships existed also between serum LDH and kidney LDH as well as between CPK in serum and heart. --Direct relationships were remarkable serum LDH and that of spleen and heart as well as between serum ACP and that of liver and heart. --Interrelationships were inverse between ACP and ALP in liver, kidney, and heart, but weak direct interrelationships were characteristic between the 2 enzymes in serum and spleen. --LDH was inversely interrelated with CPK in serum and heart.  相似文献   

18.
Serum Thyroid Hormone Concentrations Fluctuate in Cats with Hyperthyroidism   总被引：3，自引：0，他引：3  
We measured serum thyroxine (T4) and 3,3',5-triiodothyronine (T3) concentrations in hyperthyroid cats (hourly for 10 hours in 14 cats, and daily for 15 days in seven cats) to assess fluctuation in thyroid hormone levels. Over the 10-hour study period the coefficient of variation (CV) for serum T4 and T3 concentrations ranged from 6.4-22.6% (mean = 12.0 +/- 4.8%) and from 9.6-33.1% (mean = 17.5 +/- 6.3%), respectively. During the 15-day study period, CV for serum T4 ranged from 6.6-34.8% (mean = 18.4 +/- 9.3%), while CV for serum T3 ranged from 7.8-31.0% (mean = 20.1 +/- 8.6%). These CV values were significantly higher than the expected intra-assay CVs (T4 assay, 5.1%; T3 assay, 7.7%). In addition, some of the cats with mild hyperthyroidism showed one or more normal serum T4 and T3 values during the course of the respective study periods. There was no specific time during the 10-hour study period at which the cats consistently showed peak serum T4 or T3 concentrations. These results suggest that serum thyroid hormone concentrations are subject to a degree of fluctuation that exceeds the usual assay variation, and that cats with mild hyperthyroidism can, at a given time, exhibit normal serum T4 and T3 values. Therefore, a diagnosis of feline hyperthyroidism should not be excluded on the basis of the finding of a single normal serum T4 or T3 value in a cat with clinical signs and physical examination findings consistent with the disease.  相似文献   

19.
This study examined the influence of bovine growth hormone (bGH) on liver and kidney thyroxine-5'-monodeiodinase activity (TMA) in growing beef cattle. In a preliminary trial (trial 1), tissue samples were obtained at slaughter from two placebo-injected and two bGH-injected (29.2 IU/day for 14 days before slaughter) Hereford heifers (398 kg avg slaughter wt), with one heifer on each treatment fed at either 1.0 or 2.0 times maintenance energy requirement. In a second trial, tissue and plasma samples were obtained from six placebo-injected and six bGH-injected (29.2 IU/day for 19 days) Hereford steers (331 kg avg slaughter wt) fed at 1.8 times maintenance energy requirement. In a third trial, liver tissue and plasma samples were obtained from 14 Angus X Hereford steers (270 kg avg wt) fed either an 8% protein, 1.96 Mcal-metabolizable energy/kg diet (8 steers) or a 14% protein, 2.67 Mcal-metabolizable energy/kg diet (6 steers) in association with acute administration of bGH. Half the steers in each group were given two injections per steer of either placebo or 37.8 IU bGH at 24-hr intervals and slaughtered 24 hr after the second injection. Units of tissue TMA in all trials were measured at slaughter; one unit defined as 1 ng T3 generated/hr/mg protein during incubation with T4 (1.3 fM). In trial 1, TMA in liver and kidney was 2.86 and 1.21 times greater, respectively, for bGH than for placebo treatments. In trial 2, units of TMA in liver homogenates were higher (P less than .05) for bGH (4.14) than for placebo (2.83) treatments and higher (P less than .02) in liver microsomal preparations for bGH (32.7) than for placebo (27.3) treatments. Units of TMA in kidney homogenates were also higher (P less than .10) for bGH (1.48) than for placebo (.87) treatments and higher (P less than .02) in kidney microsomal preparations for bGH (23.0) than for placebo (16.4) treatments. Following acute injection of bGH (trial 3), units of TMA in liver homogenates were higher (P less than .01) for bGH (2.5) than for placebo (1.8) treatments. Plasma T4 (70.0 vs 73.5 ng/ml) and T3 (1.08 vs 1.18 ng/ml) concentrations appeared slightly higher in bGH-treated steers (trial 2) and increased by 20 and .5 ng/ml, respectively, (P less than .01) within 6 to 12 h after acute injection of bGH (trial 3). Concurrently, plasma TSH concentration increased following acute GH injection (trial 3).(ABSTRACT TRUNCATED AT 400 WORDS)  相似文献   

20.
An experiment was conducted to estimate the relative bioavailability of inorganic Se sources based on tissue Se deposition following supplementation at high dietary levels. Twenty-eight crossbred wethers averaging 50 kg initial weight were assigned randomly to seven treatments that were fed for 10 d. The basal diet contained .18 mg/kg Se (DM basis). Dietary Se was added at 0, 3, 6 or 9 mg/kg as reagent grade sodium selenite (Na2SeO3) and 6 mg/kg from either calcium selenite (CaSeO3), Na2SeO3 + fumed amorphous carrier or sodium selenate (Na2SeO4). There were four sheep per treatment group, housed in individual, raised pens with slatted floors. Daily feed intake was restricted to 1,200 g and tap water was available ad libitum. The basal diet was fed for a 10-d adjustment period, then sheep were fed experimental diets for 10 d. At the termination of the experiment, blood samples were taken; sheep were stunned and killed, and livers and kidneys were removed and frozen for Se analysis. There was a linear (P less than .001) uptake of Se in liver, kidney and serum. The CaSeO3 and Na2SeO4 sources resulted in greater (P less than .05) Se concentrations in liver and kidney than did Na2SeO3, but these differences were not significant when the analyzed dietary Se concentrations were used as a covariate in the statistical model. Based on linear and multiple linear regression slopes and average increases in serum, liver and kidney Se concentrations, estimated relative bioavailability values corrected for analyzed dietary concentration, were 100, 101, 90 and 133 for Na2SeO3, CaSeO3, Na2SeO3 + carrier and Na2SeO4, respectively.  相似文献   

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