共查询到20条相似文献，搜索用时 65 毫秒
1.
Identification and characterization of the protein encoded by the human N-myc oncogene 总被引：22，自引：0，他引：22
D J Slamon T C Boone R C Seeger D E Keith V Chazin H C Lee L M Souza 《Science (New York, N.Y.)》1986,232(4751):768-772
The human N-myc gene is related to the c-myc proto-oncogene, and has been shown to have transforming potential in vitro. Many studies have reported amplification of N-myc in human neuroblastoma and retinoblastoma cell lines. In primary tumors, amplification of the gene was found to correlate directly with behavior of the tumor. Specific restriction fragments of a partial complementary DNA clone of N-myc from LA-N-5 human neuroblastoma cells were placed into a bacterial expression vector for the purpose of producing antigens representative of the N-myc protein. Rabbits immunized with these antigens produced antisera that recognized a protein of 62-64 kilodaltons in neuroblastoma cells. By several criteria, this protein appears to be part of the same proto-oncogene family as the c-myc protein. Moreover, the antisera to fragments of this protein were capable of histochemically identifying malignant cells in clinical specimens. 相似文献
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Amplification of N-myc in untreated human neuroblastomas correlates with advanced disease stage 总被引：89，自引：0，他引：89
G M Brodeur R C Seeger M Schwab H E Varmus J M Bishop 《Science (New York, N.Y.)》1984,224(4653):1121-1124
A domain of DNA designated N-myc is amplified 20- to 140-fold in human neuroblastoma cell lines but not in cell lines from other tumor types. N-myc has now been found to be amplified in neuroblastoma tissue from 24 of 63 untreated patients (38 percent). The extent of amplification appears to be bimodal, with amplification of 100- to 300-fold in 12 cases and 3- to 10-fold in 10 others. Amplification was found in 0 of 15 patients with stage 1 or 2 disease, whereas 24 of 48 cases (50 percent) with stage 3 or 4 had evidence of N-myc amplification. These data indicate that N-myc amplification is a common event in untreated human neuroblastomas. Furthermore, N-myc amplification is highly correlated with advanced stages of disease (P less than 0.001) and with the ability to grow in vitro as an established cell line, both of which are associated with a poor prognosis. 相似文献
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Amitotic neuroblastoma cells used for neural implants in monkeys 总被引：1，自引：0，他引：1
D M Gash M F Notter S H Okawara A L Kraus R J Joynt 《Science (New York, N.Y.)》1986,233(4771):1420-1422
The potential utility of cultured neuroblastoma cells as donor tissue for neutral implants into the mammalian brain has been examined. Cells from a human neuroblastoma cell line, IMR-32, were labeled with [3H]thymidine and chemically rendered amitotic. These differentiated IMR-32 cells were grafted into the hippocampi of five adult African Green monkeys, and graft survival was evaluated for up to 270 days after transplantation. Autoradiographically labeled grafted cells were identified in four animals. Processes from grafted cells could be followed for distances of up to 150 micrometers into the host brain. No evidence for neoplastic growth of the transplant was found. Thus, grafted neuroblastoma cells can survive for prolonged periods in the primate brain and may serve as a practical source of donor tissue for neural implants. 相似文献
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Tyramine-H3: deaminated metabolites in neuroblastoma tumors and in continuous cell line of a neuroblastoma 总被引：2，自引：0，他引：2
Neuroblastoma tumors, as well as cultured cells of neuroblastoma, contain high monoamine oxidase activity. The major deaminated metabolite of tyramine-H(3) in the incubation mixtures with the tumors or with the cultured cells is p-hydroxyphenylacetaldehyde. Upon addition of reduced nicotinamide-adenine dinucleotide phosphate, the aldehyde was further metabolized by the reductive pathway to p-hydroxyphenylethanol, whereas upon addition of nicotinamide-adenine dinucleotide phosphate the aldehyde was only metabolized to a minor extent by the oxidative pathway to p-hydroxyphenylacetic acid. Aldehyde dehydrogenase activity is very low in the neuroblastoma tumors and in the cultured neuroblastoma cells. The generation of aldehydes and alcohols by the action of monoamine oxidase suggests that the deaminated metabolites of biogenic amines might exhibit some toxic effects in neuroblastoma patients. 相似文献
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[目的]研究红松种鳞多酚对癌细胞体外增殖的抑制作用.[方法]分别采用不同浓度红松多酚提取液对人骨髓神经母细胞瘤细胞株SH-SY5Y、人肺腺癌细胞株A549、人皮肤癌细胞株A375、人肝癌细胞株HepG2、人卵巢癌细胞株SKOV3这5种常见的肿瘤细胞进行试验,采用MTT法检测体外细胞增殖抑制率.[结果]红松多酚提取物对SH-SY5Y、HepG2、SKOV3的抑制作用不明显,而对A549、A375均有抑制效果.[结论]在红松多酚提取液固形物含量为0.4 mg/ml时,其对A549细胞抑制效果最佳,在该浓度下,红松多酚提取液对人肺腺癌细胞A549的抑制率可达到55％. 相似文献
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Growth regulation of human melanocytes: mitogenic factors in extracts of melanoma, astrocytoma, and fibroblast cell lines 总被引：5，自引：0，他引：5
Melanocytes derived from fetal or adult skin do not propagate in vitro unless cultured in the presence of factors such as 12-O-tetradecanoylphorbol 13-acetate (TPA). In a search for physiological factors regulating the growth of melanocytes, extracts of various cultured cell types were tested. Factors produced by melanoma and astrocytoma cell lines support continued proliferation of melanocytes in the absence of TPA. WI-38, a fibroblast cell line derived from human embryonic lung, was the most active source of melanocyte growth factors. No melanocyte growth-promoting activity was found in extracts of cultured neuroblastoma, renal cancer, normal keratinocytes, or renal epithelium. Nerve growth factor, epidermal growth factor, melanocyte-stimulating hormone, transforming growth factor-beta, and platelet-derived growth factor did not have growth-promoting activity for melanocytes. The presence of melanocyte growth factors and TPA together resulted in the strongest mitogenic activity for melanocytes, permitting the recovery (at 20 days) of 4 to 20 times as many cells as in growth factor or TPA alone. 相似文献
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Depolarization and muscarinic excitation induced in a sympathetic ganglion by vasoactive intestinal polypeptide 总被引：2，自引：0，他引：2
The effects of vasoactive intestinal polypeptide (VIP) in the superior cervical ganglion of the cat were studied in vitro and in vivo with sucrose gap and multiunit recording, respectively. At a dose of 0.03 to 0.12 nanomole, VIP produced a dose-dependent, prolonged (3 to 15 minutes) depolarization of the ganglion and enhanced the ganglionic depolarization elicited by the muscarinic agonist acetyl-beta-methylcholine. At a dose of 1.8 to 10 nanomoles, the peptide enhanced and prolonged the postganglionic discharge elicited by acetyl-beta-methylcholine, enhanced muscarinic transmission in ganglia treated with an anticholinesterase agent, and enhanced the late muscarinic discharge elicited by acetylcholine. VIP did not affect the early nicotinic discharge elicited by acetylcholine or by electrical stimulation of the preganglionic nerve. It is concluded that VIP has a selective facilitatory action on muscarinic excitatory mechanisms in the superior cervical ganglion of the cat. 相似文献
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Vitamin D3: induction of calcium-binding protein in embryonic chick intestine in vitro 总被引：2，自引：0，他引：2
Induction of the synthesis of calcium-binding protein in chick embryonic intestine maintained in vitro was accomplished by simply adding vitamin D(3) to the culture medium. Accompanying the induction of this protein, there was enhanced radiocalcium uptake by the intestine. These observations represent the first demonstration of an in vitro physiological effect of vitamin D(3) on the calcium absorptive mechanism of the intestine. 相似文献
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Neuroblastoma: drug-induced differentiation increases proportion of cytoplasmic RNA that contains polyadenylic acid 总被引：2，自引：0，他引：2
The production of cytoplasmic RNA that contains polyadenylic acid is increased, relative to total cytoplasmic RNA, in a neuroblastoma clone, NBE-(A), after induction of differentiation by 4-(3-butoxy-4-methoxybenzyl)-2-imidazolidinone, an inhibitor of adenosine 3',5'-monophosphate phosphodiesterase. The amount of RNA that contains polyadenylic acid in cytoplasm may be greater in such differentiated neuroblastoma cells than in proliferating control cells. 相似文献
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H F Clark 《Science (New York, N.Y.)》1978,199(4333):1072-1075
Several strains of attenuated rabies virus lacking the capacity to kill adult mice acquired a high lethal potential for mice after one to five serial passages in murine or human neuroblastoma cells. The virulence acquired after passage in neuroblastoma cells is a stable genetic trait retained during subsequent passage of viruses in nonneuroblastoma cell systems. 相似文献
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草莓瘦果离体培养对种子发芽率的影响 总被引：5，自引：0，他引：5
以草莓栽培品种“硕香”和黄毛草莓、森林草莓、西藏草莓等野生种的瘦果进行离体培养试验 ,结果表明 :采用切开瘦果的方法进行离体培养可提高种子发芽率 ,且在培养 10d后发芽率即达到最高值 ;不同激素浓度配比的MS培养基对草莓种子发芽率影响不大 ;栽培品种在培养基上作离体培养优于在湿滤纸上的培养 ;草莓离体培养的发芽率与基因型有关 ,栽培品种与野生种存在显著差异 ;用花后 2 1d的鲜果作切开瘦果离体培养时发芽率即接近 10 0 % ,花后 7d果实的瘦果培养也获得了少量再生植株 相似文献
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3种生长调节物质对生菜不定芽直接再生的影响 总被引：3，自引：0，他引：3
应用“３１１－Ｂ”回归最优设计研究ＡｇＮＯ３，ＡＢＡ，ＡＳＡ对生菜离体培养不定芽直接再生的影响。结果表明，ＡｇＮＯ３是良好的乙烯抑制剂，与ＡＢＡ配合使用可明显抑制愈伤产生，大大促进不定芽的直接再生；而ＡＳＡ未曾抑制愈伤产生，不是乙烯抑制剂。 相似文献
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以‘青岛百合’为试验材料,研究组培微环境中NAA、蔗糖、活性炭、矮壮素及光照/黑暗条件等因素对‘青岛百合’生根的影响.结果表明:蔗糖对‘青岛百合’鳞茎的膨大影响较大,添加60g/L蔗糖溶液时,鳞茎长势最好;黑暗处理或添加3g/L活性炭可创造根系生长的最佳黑暗微环境,有助于促进根原基的分化,能提前生根2～3d;2g/L矮壮素有助于鳞茎干物质含量的增加累积,提高增强组培苗抗性;1/2MS+0.1mg/L NAA+60g/L蔗糖溶液+3g/L活性炭或进行暗处理可在短时间内获得大量组培苗;1/2 MS+0.1mg/L NAA+60g/L蔗糖+2g/L矮壮素+光照培养可获得健壮的组培苗. 相似文献
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LI Qing-zhang HAO Yan-hong GAO Xue-jun GAO Wen-xue ZHAO Bing 《中国农业科学(英文版)》2007,6(8):1009-1017
To study the target of benzimidazole carbamate drugs against Cysticerci cellulosae and give a theoretical basis for type evolution and new drug design, the changes of key enzyme activities and metabolite contents in the pathway of energy metabolism in C. cellulosae in vitro and in vivo were tested with albendazole and oxfendazole, respectively. Both albendazole and oxfendazole inhibited the pathways of anaerobic glycolysis, partial inversed tricarboxylic acid cycle of Taenia Solium oncosphere, immature and mature Cysticerci in vitro, and immature and mature Cysticerci in vivo to a certain degree, and enhanced fat decomposing, amino acid decomposing, xanthine decomposing metabolism, and on the other hand, the absorption of glucose was hindered; furthermore, both albendazole and oxfendazole inhibited the activities of the fumaric reductase （FR） complex noncompetently in vitro. Benzimidazole carbamate drugs could inhibit the activities of FR complex noncompetently and hinder the absorption of glucose. 相似文献
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Wing disks from the last larval instar of the Indian meal moth, Plodia interpunctella (Hübner), were successfully cultured in modified Grace's medium. 20-Hydroxyecdysone induced cuticle deposition in these disks in vitro. This response was enhanced by treating the medium with larval fat body and was inhibited by application of juvenile hormone. 相似文献
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Examination of ionic membrane currents in a voltage-clamped neuronal cell line derived from the mouse C1300 neuroblastoma disclosed four kinetically different components: sodium, potassium, calcium, and leakage current. The kinetics, voltage dependence, and pharmacological properties of the sodium and potassium currents qualitatively resemble those of the corresponding currents in squid giant axon and frog myelinated nerve fiber, suggesting that the molecular structures of the sodium and potassium channels in neuroblastoma are similar to those of the non-mammalian preparations. 相似文献
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