首页 | 本学科首页   官方微博 | 高级检索  
相似文献
 共查询到20条相似文献，搜索用时 484 毫秒
1.
A macromethod and a semimicromethod were developed to measure erythrocyte acetylcholinesterase activity in cattle, sheep, goats, horses, dogs, and swine, and to measure plasma cholinesterase activity in horses, dogs, and swine. Comparison of the 2 methods with erythrocytes of sheep, cattle, goats, and horses indicated both methods gave similar results. They can be done in a shorter time and are more sensitive than Michel's method. Normal deltapH values per minutes, with standard deviations for blood cholinesterase activity of animals of different ages, sexes, breeds, and species, were: 0.76 +/- 0.12/30; 0.65 +/- 0.10/15; 0.69 +/- 0.19/45; 0.78 +/- 0.11/45; 0.63 +/- 0.11/45; and 0.71 +/- 0.06/25 for sheep, cattle, goats, horses, dogs, and swine erythrocyte acetylcholinesterase, respectively; and 0.66 +/- 0.18/20; 0.67 +/- 0.20/30, and 0.46 +/- 0.05/60 for horses, dogs, and swine plasma cholinesterase, respectively. It was shown that either the chloride or the iodide salt of acetylcholine can be used as the enzyme substrate. tin blood samples stored at 5 C for 24 hours, there was no significant change of the enzymatic activity.  相似文献   

2.
Serum samples collected on a single occasion from cattle, sheep and goats at sites in all 10 regions of Ghana were tested for antibodies to Ehrlichia (previously Cowdria) ruminantium, the causative agent of heartwater, by polyclonal competitive ELISA (PC-ELISA). The survey revealed the presence of heartwater-exposed ruminants throughout the country, with local seroprevalence up to 100%. Seronegative, and therefore presumably susceptible, animals were also present in all regions, in some areas in numbers high enough to indicate local endemic instability. Overall seroprevalences in cattle, sheep and goats were 61, 51 and 28% respectively, and were generally higher in the northern part of the country and lower in the forest zone. Amongst animals over 1 year old, two thirds of cattle and sheep, and around one third of goats throughout the country had been exposed to E. ruminantium. In the north, seroprevalence in sheep sampled with and without cattle was similar, whereas in the south seroconversion rates in sheep were significantly higher in areas where cattle were present.  相似文献   

3.
A questionnaire-survey data indicated that 26% of 276 farmers reported the presence of respiratory disease in their herds in 2001. The incidence was perceived as "high" in small ruminants and camels, but as "low" in cattle. Simultaneously, 2815 serum samples from camels (n=628), cattle (n=910), goats (n=442) and sheep (n=835) were tested. The peste des petits ruminants (PPR) antibody seroprevalence was 3% in camels, 9% in cattle, 9% in goats and 13% in sheep. The highest locality-specific seroprevalences were: camels 10%, cattle 16%, goats 22% and sheep 23%. The animals had not been vaccinated against rinderpest or PPR. Antibody seroprevalences detected in camels, cattle, goats and sheep confirmed natural transmission of PPR virus under field conditions.  相似文献   

4.
AIM: To measure the background concentration of phylloerythrin in plasma from clinically healthy sheep, goats, cattle and horses on pasture. METHODS: Blood samples were taken from 34 sheep of the Dala breed, 20 female Norwegian dairy goats, 35 Norwegian Red cows and 34 horses of different breeds. All animals were grazing green pasture when blood samples were taken. Blood samples were collected from each of four clinically healthy newborn lambs, goats, calves and foals, and pooled into one sample per species. Plasma samples were analysed for phylloerythrin by fluorescence spectroscopy, using a Perkin-Elmer LS-50B luminescence spectrometer equipped with a red-sensitive photomultiplier. The fluorescence spectra of phylloerythrin in plasma from the adult ruminants were compared with those in plasma from the neonatal ruminants, to which a known concentration of phylloerythrin had been added. RESULTS: Plasma obtained from the adult ruminants had spectral characteristics similar to those of phylloerythrin, namely weak emission peaks at 650 and 711 nm, when excited at 425 nm. Emission spectra obtained from plasma from the neonatal ruminants showed no fluorescence at these wavelengths. On average, 0.012 (SD 0.004), 0.06 (SD 0.04), and 0.05 (SD 0.03) micromol/l phylloerythrin were present in plasma samples from the sheep, goats, and cattle, respectively. The fluorescence spectra of plasma from the newborn foals were similar to spectra of plasma from adult horses, with weak emission at 669 nm. CONCLUSION: Small concentrations of phylloerythrin were detected in plasma from clinically healthy sheep, goats and cattle, but none could be detected in plasma from clinically healthy horses.  相似文献   

5.
Esterases are a large group of enzymes found in plasma (or serum) and tissues, which hydrolyse a number of xenobiotics. Some of these enzymes have not yet been measured in tropical domestic animals. In the present work, we have measured the activities of three esterases, viz. aspirin esterase, cholinesterase and nitrophenylacetate esterase in the plasma and livers of male and female camels, cattle, sheep and goats. The results indicate that the enzyme activities are highest in the goat, followed by sheep and cattle (which showed broadly similar activities), and lowest in camels. Gender had no statistically significant effect, although males exhibited about 5-8% more enzyme activity in each of the four species, than females.  相似文献   

6.
The prevalence of malignant catarrhal fever (MCF) virus infection in cattle, sheep and goat populations and also the prevalence of recovered and chronic MCF cases in north-western Turkey are reported. A total of 600 animals, 200 individuals of each species, located in four provinces were sampled between December 2003 and July 2005. A monoclonal antibody-based competitive inhibition (ci) ELISA were used to detect infection status of the animals. Detected antibody prevalence was 97.5%, 96.0% and 15.0% in sheep, goats and cattle, respectively. These results showed that MCF related gammaherpesvirus infections are common in north-western Turkey. There was no significant difference between prevalences detected in sheep and goats, as well as various breeds of these species. There was also no significant difference among locations. Results of this study show that sheep and goats may equally be important in the epidemiology of MCF in Turkey. Seropositivity against MCF agents among cattle was 15.0%. The results indicate that MCF infections may be maintained in intensively managed cattle herds having no close contact with small ruminants.  相似文献   

7.
AIM: To measure the background concentration of phylloerythrin in plasma from clinically healthy sheep, goats, cattle and horses on pasture.

METHODS: Blood samples were taken from 34 sheep of the Dala breed, 20 female Norwegian dairy goats, 35 Norwegian Red cows and 34 horses of different breeds. All animals were grazing green pasture when blood samples were taken. Blood samples were collected from each of four clinically healthy newborn lambs, goats, calves and foals, and pooled into one sample per species. Plasma samples were analysed for phylloerythrin by fluorescence spectroscopy, using a Perkin-Elmer LS-50B luminescence spectrometer equipped with a red-sensitive photo- multiplier. The fluorescence spectra of phylloerythrin in plasma from the adult ruminants were compared with those in plasma from the neonatal ruminants, to which a known concentration of phylloerythrin had been added.

RESULTS: Plasma obtained from the adult ruminants had spectral characteristics similar to those of phylloerythrin, namely weak emission peaks at 650 and 711 nm, when excited at 425 nm. Emission spectra obtained from plasma from the neonatal ruminants showed no fluorescence at these wavelengths. On average, 0.012 (SD 0.004), 0.06 (SD 0.04), and 0.05 (SD 0.03) μmol/l phylloerythrin were present in plasma samples from the sheep, goats, and cattle, respectively. The fluorescence spectra of plasma from the newborn foals were similar to spectra of plasma from adult horses, with weak emission at 669 nm.

CONCLUSION: Small concentrations of phylloerythrin were detected in plasma from clinically healthy sheep, goats and cattle, but none could be detected in plasma from clinically healthy horses.  相似文献   

8.
Whole blood cholinesterase was measured using acetyl-, butyryl- and propionylthiocholine as substrates in 10 healthy adult dogs, cats, horses, pigs, goats, sheep and cows, in order to determine and characterise the cholinesterase activity in whole blood of the main domestic animals. An in vitro exposure test with two anticholinesterase compounds, the organophosphate insecticide coumaphos and the carbamate insecticide imidocarb, was also performed. In whole blood of ruminants and pigs, acetylthiocholine yielded the highest cholinesterase activity and other substrates were poorly hydrolysed; in dogs and cats, although acetylthiocholine showed the highest cholinesterase activity, butyryl- and propionylthiocholine also produced high cholinesterase values; in horses, propionylthiocholine was the substrate that yielded the highest cholinesterase activity, closely followed by butyrylthiocholine. All within- and between-run coefficients of variation observed in whole blood samples were less than 5 and 7 per cent, respectively, except when butyrylthiocholine was used as substrate in ruminant blood samples. Butyryl- and propionylthiocholine were the substrates that yielded higher inhibitions after coumaphos exposure, whereas the use of acetylthiocholine showed the highest cholinesterase inhibition after imidocarb exposure. The use of at least two substrates (acetyl and butyrylthiocholine) is recommended for whole blood cholinesterase analyses in domestic animals since it will allow monitoring of both acetyl- and butyrylcholinesterase activities, respectively, and a more accurate detection of exposure to anticholinesterase compounds. However, acetylthiocholine could be used as a unique substrate for whole blood cholinesterase determination in porcine and ruminant samples since butyrylcholinesterase activity is very low in these species. Additionally, propionylthiocholine could be used as an alternative substrate to butyrylthiocholine in horse whole blood samples.  相似文献   

9.
Monoclonal antibody-based competitive ELISA (C-ELISA) has been used for the specific measurement of antibodies to peste des petits ruminants (PPR) viruses in sheep, goats, cattle and Buffalo. Serum samples from sheep (n = 232), goats (n = 428), cattle (n = 43), buffalo (n = 89) were tested. The animals had not been vaccinated against rinderpest or PPR. Findings suggested that the sero-positive cases were significantly higher in sheep (51.29%) than in goats (39.02%) (P = 0.002). The overall sero-prevalence of PPRV in small ruminants was 43.33%. The PPR antibodies seroprevalence was 67.42% in buffalo and 41.86% in cattle which was significantly higher in buffalo (P = 0.005). The overall sero-prevalence of PPRV in large ruminants was 59.09%. Cattle and buffalo sera showed a high prevalence of antibody against PPR virus which may explain the difficulty experienced in achieving high post-vaccination immunity levels against rinderpest. Because antibodies against PPR virus are both cross-neutralizing and cross-protective against rinderpest virus, further vaccination in the presence of antibodies against PPR virus may be a waste of national resources. It was also suggested that antibodies to PPR virus could prevent an immune response to the rinderpest vaccine. This paper presents serological evidence for the transmission of PPR virus from sheep and goats to cattle and buffalo and highlights the need to include PPR serology in the sero-monitoring programme to give a better indication of national herd immunity of sheep and goats against PPR.  相似文献   

10.
A serological survey on dermatophilosis was carried out amongst sheep and goats in Kaduna State of Nigeria. Sera were obtained from slaughter animals and from sheep kept on an isolated ranch. The percentage of seropositive animals was 28.0 in slaughter sheep, 0.0 in sheep kept on the ranch, and 23.2 in slaughter goats. The high prevalence of D. congolensis antibodies among small ruminants compares well with the level of prevalence reported of cattle of cattle and calls for a concerted government effort for the control of the disease.  相似文献   

11.
The prevalence of Coxiella burnetii antibodies in domestic ruminants in Albania has been investigated. A total of 1656 serum samples taken from sheep, goats, and cattle housed on farms located in 20 different districts were tested by ELISA for the presence of specific antibodies to C. burnetii phase I and II antigens. Specific IgG antibodies were detected in 9.1% of the animals from both lowland and mountainous areas. In total, a slightly higher percentage of antibodies was detected in sheep and goats (9.8%) than in cattle (7.9%).  相似文献   

12.
This study was conducted between April 2000 and March 2001, in 12-month period. During the study, local slaughterhouses were visited periodically for 1 year to examine the internal organs (livers, lungs, spleens and hearts) for the presence of cysts and total 1355 cattle, 218 sheep and 104 goats were examined for the cystic echinococcosis (CE). It was found that 13.5% of cattle, 26.6% of sheep and 22.1% of goats were infected with this disease. While cysts in cattle (P < 0.001) and goats (P > 0.05) were found mostly in lungs (88.5 and 82.6%, respectively), but they were mostly found in livers (P > 0.05) in sheep. In addition to this, three spleens and one heart in cattle were infected with CE. In this study, the prevalence of CE and the number of cysts in ruminants were found different when the cattle, sheep and goats examined were stratified based on age. The prevalence and the number of cysts increased with age approaching an asymptotic prevalence of one in the oldest animals (P < 0.05). The number of cysts in cattle, sheep and goats were increasing at a rate of 0.31, 0.63 and 0.42/year, respectively. The economic decrease in the value of the carcasses because of the discarded liver and lung as a result of CE was estimated as 1.1% (7.5 US dollars per cattle) for cattle, 4.37% (3.2 US dollars per sheep) for sheep and 4.26% (2.9 US dollars per goat) for goats. The minimum total loss for all infected animals was determined to be 583 US dollars in infected animals, based on the market prices in the year 2002.  相似文献   

13.
Giemsa-stained thin blood smears prepared monthly from cattle, sheep and goats in the Greater Accra region of Ghana between May 1994 and December 1996 were examined for presence of tick-borne haemoparasites. The majority of animals were less than 2 months old at the start of the survey. Monthly and cumulative incidences are presented of Anaplasma sp., Babesia bigemina, Borrelia sp., Eperythrozoon sp., Theileria mutans and Theileria velifera in cattle, Anaplasma sp., Borrelia sp., and Theileria sp. in sheep, and Anaplasma sp. in goats. T. mutans was the commonest parasite in cattle, with 100% incidence in calves by 10 months of age, and Anaplasma was commonest in small ruminants. The relative prevalence of these haemoparasites in blood smears from cattle, sheep and goats sampled on a single occasion at sites in all 10 regions of Ghana was found to be similar, though actual infection rates were lower. Packed cell volume (PCV) measurements from the sampled animals are also presented; no seasonal trends were evident in the PCV of the cattle, sheep and goats sampled monthly. In animals sampled on a single occasion, mean PCV was significantly higher in cattle and sheep without detectable haemoparasite infection, and in cattle was lowest in animals positive for both Babesia and Anaplasma, while there was no difference in mean PCV levels between parasitised and non-parasitised goats.  相似文献   

14.
Recent studies have identified a phenomenon in which ciliated protozoa engulf Salmonella and the intra-protozoal environment hyperactivates virulence gene expression and provides a venue for conjugal transfer of antibiotic resistance plasmids. The former observation is relegated to Salmonella bearing the SGI1 multiresistance integron while the latter phenomenon appears to be a more generalized event for recipient Salmonella. Our previous studies have assessed virulence gene hyperexpression only with protozoa from the bovine rumen while conjugal transfer has been demonstrated in rumen protozoa from cattle and goats. The present study examined virulence gene hyperexpression for Salmonella exposed to rumen protozoa obtained from cattle, sheep, goats, or two African ruminants (giraffe and bongo). Conjugal transfer was also assessed in these protozoa using Salmonella as the recipient. Virulence gene hyperexpression was only observed following exposure to the rumen protozoa from cattle and sheep while elevated virulence was also observed in these animals. Conjugal transfer events were, however, observed in all protozoa evaluated. It therefore appears that the protozoa-based hypervirulence is not universal to all ruminants while conjugal transfer is more ubiquitous.  相似文献   

15.
This study was conducted between April 2000 and March 2001, in 12‐month period. During the study, local slaughterhouses were visited periodically for 1 year to examine the internal organs (livers, lungs, spleens and hearts) for the presence of cysts and total 1355 cattle, 218 sheep and 104 goats were examined for the cystic echinococcosis (CE). It was found that 13.5% of cattle, 26.6% of sheep and 22.1% of goats were infected with this disease. While cysts in cattle (P < 0.001) and goats (P > 0.05) were found mostly in lungs (88.5 and 82.6%, respectively), but they were mostly found in livers (P > 0.05) in sheep. In addition to this, three spleens and one heart in cattle were infected with CE. In this study, the prevalence of CE and the number of cysts in ruminants were found different when the cattle, sheep and goats examined were stratified based on age. The prevalence and the number of cysts increased with age approaching an asymptotic prevalence of one in the oldest animals (P < 0.05). The number of cysts in cattle, sheep and goats were increasing at a rate of 0.31, 0.63 and 0.42/year, respectively. The economic decrease in the value of the carcasses because of the discarded liver and lung as a result of CE was estimated as 1.1% ($7.5 per cattle) for cattle, 4.37% ($3.2 per sheep) for sheep and 4.26% ($2.9 per goat) for goats. The minimum total loss for all infected animals was determined to be $583 in infected animals, based on the market prices in the year 2002.  相似文献   

16.

West Nile virus (WNV) is a mosquito-borne disease, usually present as a symptomatic disease but can cause various clinical signs ranged from mild fever to severe encephalitis and death in various animals and humans. In Egypt, the epidemiological data about WNV infection in different animal species particularly in domestic ruminants are scarce. The present study aimed to investigate the seroprevalence of WNV in cattle, buffalo, camel, sheep, and goats at some Governorates northern Egypt. In total, 360 serum samples (100 cattle, 50 buffalo, 50 camels, 85 sheep, and 75 goats) were examined using ELISA. The results revealed that the seroprevalence of WNV among ruminants was highly significant (P = 0.03) at Kafr El Sheikh Governorate (17.6%) in comparison with other the Governorates. Besides, the seroprevalence of WNV antibodies significantly differed between the examined species (P = 0.0001); it was 22%, 0%, 40%, 3.5%, and 5.3% in cattle, buffalo, camel, sheep, and goats, respectively. This is the first study to confirm that domestic ruminants act as a reservoir in the epidemiology of WNV infection and represent a risk for human and equine infections in Egypt.

  相似文献   

17.
A biomonitoring protocol, using blood cholinesterase (ChE) activity in livestock as a monitor of potential organophosphate nerve agent exposure during the planned destruction of US unitary chemical warfare agent stockpiles, is described. The experimental design included analysis of blood ChE activity in individual healthy sheep, horses, and dairy and beef cattle during a 10- to 12-month period. Castrated and sexually intact males, pregnant and lactating females, and adult and immature animals were examined through at least one reproductive cycle. The same animals were used throughout the period of observation and were not exposed to ChE-inhibiting organophosphate or carbamate compounds. A framework for an effective biomonitoring protocol within a monitoring area includes establishing individual baseline blood ChE activity for a sentinel group of 6 animals on the bases of blood samples collected over a 6-month period, monthly collection of blood samples for ChE-activity determination during monitoring, and selection of adult animals as sentinels. Exposure to ChE-inhibiting compounds would be suspected when all blood ChE activity of all animals within the sentinel group are decreased greater than 20% from their own baseline value. Sentinel species selection is primarily a logistical and operational concern; however, sheep appear to be the species of choice because within-individual baseline ChE activity and among age and gender group ChE activity in sheep had the least variability, compared with data from other species. This protocol provides an effective and efficient means for detecting abnormal depressions in blood ChE activity in livestock and can serve as a valuable indicator of the extent of actual plume movement and/or deposition in the event of organophosphate nerve agent release.  相似文献   

18.
The structure of the omasum was compared in 10 water buffalo (Bubalus bubalis), 10 Shorthorn cattle (Bos indicus), 9 Merino sheep (Ovis aries) and 10 cross-bred goats (Capra hircus), and the functional significance of the differences is discussed. The weight of the omasum and area of the internal laminae appeared to be smaller in Brahman cattle than the buffalo and British cattle. When compared in relation to body weight, the omasums of caprid animals were smaller than those of bovids, by a factor of 0.33-0.67 but the omasums of goats were appreciably larger than sheep. The number of laminae ranged from 33 to 35 for the sheep and goats compared with 122 to 169 for the buffalo and cattle. It is suggested that the marked difference in structure of the omasum between the small and large ruminants may influence the food intake of these animals through the effects of this gut compartment on absorption of nutrients and passage of digesta.  相似文献   

19.
A capillary flocculation test was developed to diagnose heartwater disease of ruminants. Antigen was prepared from the brains of cattle and goats highly infected with Cowdria ruminantium. Sera were obtained from experimentally infected ruminants which either recovered naturally or with the aid of oxytetracycline treatment. Antibodies were first detected one to two weeks after clinical recovery or after treatment, and persisted for periods varying between one and four weeks. Control sera collected from cattle (sheep) and goats in the Netherlands where heartwater does not occur, or from animals serologically positive for Anaplasma marginale or Eperythrozoon ovis infections, did not react to the test.  相似文献   

20.
隐孢子虫是可以感染小反刍动物的肠道原生生物,具有潜在的公共卫生学问题。隐孢子虫可引起人和动物的腹泻和肠道疾病,严重情况下会出现死亡。被感染的动物可能是人畜共患型隐孢子虫的宿主,可引起公共卫生风险、农场利润减少和动物福利等问题。隐孢子虫病已被报道是新生牛、羊等反刍动物腹泻和死亡的一个重要原因,认为是新生羔羊腹泻的第二大诱因,仅次于轮状病毒。羊隐孢子虫病在世界范围内都有发现,不同程度地威胁着人和动物的身体健康,基于此,现就隐孢子虫对羊感染的危险因素和常用检测方法,以及我国羊感染的现状进行综述,为其防控提供参考。  相似文献   

设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号