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1.
To determine the helminth fauna of wild boars, stomachs and intestines of 124, lungs of 62, and livers of 39 animals from 5 enclosures in Southern Germany were examined. The evaluation of the species composition revealed at least 13 species: Metastrongylus apri, M. salmi, M. pudendotectus, Physocephalus sexalatus, Hyostrongylus rubidus, Globocephalus longemucronatus, Ascaris suum, Trichuris suis, Oesophagostomum dentatum, O. quadrispinulatum, Fasciola hepatica, C. tenuicollis and Capillaria. The most frequently isolated species was M. salmi with 91.9%, followed by G. longemucronatus with 91.1%, M. apri with 88.7%, M. pudendotectus with 87.1%, P. sexalatus with 51.6%, O. dentatum with 46%, T. suis with 30.6%, H. rubidus with 14.5%, A. suum with 12.1% and O. quadrispinulatum with 8.1%. Differences in the prevalence of infection, determined for animals up to 1 year old, 1-2 year-old and adults, were minimal for Metastrongylus spp. and G. longemucronatus. P. sexalatus, T. suis and A. suum, however, occurred more often in 1 year old, whereas H. rubidus, O. dentatum and O. quadrispinulatum were isolated more often from adults. A correlation of the infection rate with the sex of the animals was not found.  相似文献   

2.
The mandarinfish, Synchiropus splendidus, is a small, pelagic-spawning enthic dragonet of the western Pacific. Although popular in the marine aquarium trade, little is known of its fishery or biology. All aquarium-trade animals are currently taken from the wild and the impact of heavy collecting is unknown. The specialized and selective nature of the fishery for mandarinfish is described and its potential to disrupt the mating system identified. As a possible alternative to wild capture and as an aid to sustainable exploitation, egg production and early development relevant to mariculture are described, including egg output, embryo, larva and post-settlement development to 30 days, based on live material. Egg output was determined for 40 females and ranged from 12 to 205 eggs. Embryo and larva development were rapid, with settlement occurring within 14 days at 24–26 °C, and at 3.5 mm TL. The swimbladder is retained in adults. Our limited attempts at raising the mandarinfish to settlement were encouraging and suggest an excellent potential for mariculture with implications for both conservation and improved maintenance of fish in captivity. At present, given that this species is difficult to maintain in captivity, it is only suitable for experienced aquarists. Preliminary diet information is provided. This revised version was published online in August 2006 with corrections to the Cover Date.  相似文献   

3.
Egg Quality in Wild and Broodstock Cod Gadus morhua L.   总被引:1,自引:0,他引:1  
Problems concerning egg viability or egg quality have received increasing attention, in relation to cultivation as well as to the assessment of reproduction of wild fishes. One important aspect of this paper is to discuss the use of cell morphological characteristics as indicators of egg quality in cod. Early cellular malformation rates in egg batches show a positive correlation with their later egg and larval survival, and can be used as a rough prediction of normal development and mortality rates.
The natural mortality rate offish eggs is not well understood, and observed egg viability of captive cod is are discussed in relation to egg viability in the wild. Results from recent work indicated that approximately 10% of naturally spawned cod eggs from both wild fish (planktonic samples) and from a broodstock had abnormal cleavages at the 2–128 cell stage. Eggs with observed early abnormal cell-cleavage showed very poor survival and hatching rates. For broodstock cod, egg viability was highest during the peak of the spawning season.
The North Atlantic cod may provide a good model species for studies of egg viability variations in wild and captive individuals, as its reproductive biology is well studied. It is now documented that the egg production in cod is dependent on fish age, nutritional status, batch number, and stress factors.  相似文献   

4.
The widespread belief in the superior quality of highly pigmented eggs is examined by bringing together evidence concerning the effect of the carotenoid content of salmonid eggs on their viability. It is concluded that, in rainbow trout, there is not a simple linear relation between these two quantities. There is some evidence that a critical level exists (ca. 1–3 μg carotenoid/g eggs) above which hatching percentages of more than 80% may be expected but below which hatching percentages of less than 50% tend to occur. However, the evidence is not conclusive and suitable experiments under controlled conditions have not been performed. Eggs of wild fish are frequently of higher quality than eggs of farmed fish but this difference is not necessarily caused by the large difference in egg carotenoid content but rather by the generally superior nutrition of the wild fish. The effects of egg carotenoid composition, including the artificial pigmentation of eggs with dietary canthaxanthin, on egg viability are considered, and the possible functions of egg carotenoids during embryonic development are discussed. Only one such function has been conclusively demonstrated, namely that they act as a source of pigment for the chromatophores of the alevin. Analogy with other animals suggests that β-carotene, when present, may act as a precursor of vitamin A. There is growing evidence that carotenoids may perform some undefined function in respiration of egg or alevin when oxygen is deficient.  相似文献   

5.
Epitheliocystis in Swiss brown trout (Salmo trutta) is a chlamydial infection, mainly caused by Candidatus Piscichlamydia salmonis and Candidatus Clavichlamydia salmonicola. To gain a better understanding of the temporal development of infections in wild brown trout, we investigated epitheliocystis infections during the course of the summer and autumn months of a single year (2015), and compared this to sampling points over the span of the years 2012–2014. The survey focused on tributaries (Venoge and Boiron) of the Rhone flowing in to Lake Geneva. When evaluated histologically, epitheliocystis infections were found throughout the period of investigation with the exception of the month of June. Fifty to 86 animals per sampling were investigated. Highest prevalence and infection intensities were seen in September. A correlation between epitheliocystis infection and water temperatures was not evident. Interyear comparison revealed consistent levels of prevalence and infection intensities in late summer. The absence of infections in June, combined with the consistent interyear results, indicates seasonal fluctuation of epitheliocystis infections in brown trout with a reservoir persisting during winter months from which infections can re‐initiate each year. This could either be at levels below detection limits within the brown trout population itself or in an alternative host.  相似文献   

6.
Tanabe  Atsushi  Shimizu  Risa  Osawa  Yui  Suzuki  Machi  Ito  Saki  Goto  Mutsuo  Pastene  Luis A.  Fujise  Yoshihiro  Sahara  Hiroeki 《Fisheries Science》2020,86(1):35-41
Fisheries Science - Determining the age of wild animals is very important in the study of their ecology and for stock assessment and management. In baleen whales, the most common approach for age...  相似文献   

7.
Slovenia has no history of health problems related to proliferative kidney disease (PKD) either in farmed or in wild fish. However, due to the past molecular evidence for the presence of Tetracapsuloides bryosalmonae DNA in tissues of some fish from open waters, a survey was conducted on wild salmonids that were primarily sampled for other purposes. In winter 2010–2011, specimens from a total of 244 rainbow trout, Oncorhynchus mykiss (Walbaum), and brown trout, Salmo trutta L., from 30 bodies of fresh water were examined for T. bryosalmonae using a PCR method. The sampled fish showed no clinical signs or gross pathological lesions characteristic of PKD. Nineteen (7.8%) fish from seven (23.3%) fresh waters were positive for T. bryosalmonae. The identity of PCR amplicons was confirmed by sequencing. With one exception, all the positive fish were found in waters from the regions where the average yearly temperatures and the environmental pollution are higher. This preliminary countrywide survey provided the first insight into the situation regarding T. bryosalmonae infection of wild salmonids in Slovenia.  相似文献   

8.
Abstract.— The breeding frequency, fecundity, egg hatchability, larval output and viability of pond-reared, mature Macrobrachium rosenbergii females were individually followed up during 180 d. Sexually mature prawns were maintained under controlled laboratory conditions (28.7 C and 12-h light/d) in a 190-L freshwater recirculation system and fed a formulated diet. Ovarian development, moulting and spawning events were checked daily. At least six consecutive moults were recorded for each of the 18 females. The duration of the intermoult period averaged 27.5 d and was not affected by ovarian development or spawning, but intermoult periods followed by spawning had significantly lower growth rates. From a total of 126 moulting events recorded, egg laying successfully followed 76 (60.3%) of them. The number of eggs per spawn (NES) varied from 26,587 to 74,775 for females weighing 20.0 to 55.8 g. The relationship between NES and female size (W; in g) was found to be NES = 484 + 1454W ( r 2= 0.74). Results suggest that the number of viable larvae produced per egg clutch may be increased by in vitro incubation, as the losses of eggs, which usually occur under in vivo incubation, are prevented. The present study illustrates that under adequate and stable rearing conditions, M. rosenbergii females are able to spawn up to five times during 180 d, in comparison to three to five times per year as reported for wild prawn populations.  相似文献   

9.
Banded morwong (Cheilodactylus spectabilis) are of interest for marine finfish aquaculture in temperate southern Australia. To improve their ovulatory response, adult females were implanted during the autumn spawning season with slow‐release pellets containing 0–400 μg luteinizing‐hormone‐releasing hormone analogue (LHRHa)/kg body weight within 24 h of capture from the wild. Compared to the sham control group, animals treated with LHRHa produced significantly more eggs on each day after implantation for the following 7 d (91 ± 39 and 290 ± 38 mL) and a higher proportion ovulated (8/12 and 27/27). Of fish treated with LHRHa, 93% ovulated 2 d after implantation and 79% ovulated three times at 2‐d intervals, whereas control animals showed no cyclicity of ovulation and few ovulated more than once. Egg production was highest at the first ovulation after LHRHa treatment and declined at subsequent ovulations. In a second experiment investigating the range 100–400 μg LHRHa, there was no effect of dose rate on ovulation parameters, which additionally examined implantation either immediately after capture or after a 5‐d delay. Compared to immediate implantation, a delay resulted in a lower proportion of animals that could be stripped after implantation (100 and 50%, respectively) and the volume of eggs was lower (135 ± 15 and 107 ± 10 mL). The egg quality was poor following delayed implantation, resulting in no fertilization after artificial insemination compared with immediate implantation in which fertilization and hatch rates were higher for eggs collected on Day 2 after implantation (79 ± 8% and 58 ± 9%) than on Day 4 (23 ± 7% and 15 ± 6%). Thus, it is important to implant animals as soon as possible after capture to ensure optimum egg quality. Good‐quality eggs were buoyant and spherical and had a diameter of 1050 ± 25 μm with a single pigmented oil droplet of 190 ± 9 μm. When a separate large batch of eggs collected 2 d after implantation with 100 μg LHRHa was inseminated and cultured at 18 C, larvae hatched after 63 ± 2 h at a standard length of 2.6 ± 0.4 mm. Newly hatched larvae were buoyant and transparent with only a few melanophores, eyes were nonpigmented and jaws were nonfunctional. By the fourth day, jaws were functional and eyes were fully pigmented. Utilization of the endogenous yolk and oil was completed by Day 6, and swimming commenced with exogenous feeding. Larvae, initially fed lipid‐enriched rotifers followed by Artemia, reached 8.9 ± 0.7 mm length on Day 55, after which they metamorphosed to the postlarval paperfish stage of development, 22 ± 0.9 mm on Day 100, and 43 ± 1.0 mm at 6 mo of age. The results show that treatment of wild‐caught females with slow‐release pellets containing LHRHa is effective for the production of eggs for hatchery rearing.  相似文献   

10.
The sea louse, Lepeophtheirus salmonis (Krøyer 1837), is a significant parasite of farmed salmon throughout the Northern Hemisphere. Management of on‐farm louse populations can be improved by understanding the role that wild fish play in sustaining and providing refuge for the local population of sea lice. In this study, 1,064 sticklebacks were captured. Of these animals, 176 individuals were carrying a total of 238 sea lice, yielding a prevalence and intensity of 16.5% and 1.4 lice per fish, respectively. Detailed examination of the sea lice on the three‐spined sticklebacks captured in Cobscook Bay found two L. salmonis individuals using three‐spined sticklebacks as hosts. A 2012 survey of wild fish in Cobscook Bay, Maine, found multiple wild hosts for Caligus elongatus (von Nordmann 1832), including three‐spined sticklebacks (Gasterosteus aculeatus L.), but no L. salmonis were found in this earlier study.  相似文献   

11.
Hatchery-spawned and reared abalone that are placed onto subtidal reefs to enhance native populations often suffer a high mortality. A potential source of mortality is behavioral differences of cultured and wild abalone. Controlled observations and factorial experiments in the laboratory were used to examine abalone behavior and mortality rate due to three predators — crabs, sea stars and lobsters — on small reefs in laboratory tanks. Two abalone types — cultured and wild — and two age/size classes, 0+ (8–20 mm shell length) and 1+ (25–50 mm), were used. Abalone types differed in their movement patterns, with wild animals moving rapidlyto concealed positions within the reefs and most cultured abalones remaining in their original positions for several hours. Over all experiments, there were differences in predation rates by crabs, lobsters and sea stars, with crabs consuming the most abalone and sea stars the least. Significantly more cultured than wild abalone were consumed in an experiment in which different combinations of predators were present. No consistent differences were seen in the consumption of the two size classes of abalone. Animals fresh from the hatchery suffered significantly higher mortality and were more sluggish in their responses to predators than hatchery-reared abalone that had been acclimated to laboratory conditions. The results suggest, however, that cultured abalone may acclimate relatively quickly to a new environment, which may be useful for future attempts at transplanting these animals.  相似文献   

12.
哈维弧菌(Vibrio harveyi)是水生动物的重要病原,为研究哈维弧菌溶血素基因vhh缺失后对其生物学特性的影响,该研究利用同源重组技术构建了V.harveyi 345的vhh基因缺失突变株,并比较了野生株和突变株的生物学特性变化。结果显示,vhh基因的缺失不影响菌株的生长、胞外蛋白酶分泌、过氧化氢(H2O2)和铜离子(Cu2+)的压力感应、铁的吸收利用、15种抗生素抗性和生物膜形成等生物学特性,但会导致菌株游动和涌动显著增强;另发现,vhh基因虽然在野生菌株内高表达,对绵羊红细胞却未表现出溶血活性。结果表明该基因负调控着菌株的运动能力。该研究为认识哈维弧菌vhh基因功能研究提供新的资料。  相似文献   

13.
为优化曼氏无针乌贼人工繁育技术,提高人工增殖苗种的产量与质量,采用实验生态学方法,对比分析了野生和养殖曼氏无针乌贼(Sepiella japonica)的表观形态、生化组分及其受精卵的形态差异,探究了光照周期与受精卵胶质外膜对曼氏无针乌贼胚胎发育的影响。结果显示:野生和养殖曼氏无针乌贼的表观形态、生化组分均存在显著差异,野生群体性成熟规格远大于养殖群体,肌肉和肝脏组织中的脂肪含量显著低于养殖群体(P<0.05),卵巢中的蛋白质含量则显著高于养殖群体(P<0.05);养殖亲体所产黑卵在12D12L光照周期条件下孵化率最高,所产白卵在24D和24L条件下孵化率最高,野生亲体所产卵在24D和12D12L条件下孵化率最高;随光照时间的延长,受精卵的平均孵化时间具有先增加后减小的趋势,表明光照周期是影响曼氏无针乌贼胚胎发育的重要因素;实验发现,将受精卵胶质外膜剥离处理后,养殖亲体所产黑白卵的平均孵化时间具有减少的趋势,但孵化率显著下降(P<0.05)。综上所述,建议在曼氏无针乌贼人工苗种生产过程中,尽量选用大规格野生乌贼作为繁育亲体。此外,在受精卵孵化期间,应对孵化池进行适当遮光处理,避免受精卵长时间暴露于强光照环境中,以提高孵化率和仔乌成活率。本实验研究结果可为曼氏无针乌贼人工苗种繁育技术优化提供参考。  相似文献   

14.
胶州湾移植底播菲律宾蛤仔的生长和死亡特性   总被引:3,自引:0,他引:3       下载免费PDF全文
2004年5月至2005年4月对胶州湾菲律宾蛤仔(Rudimpes philippinarum)底播增殖区进行了逐月定点采样,将样品带回放于实验室内水族箱暂养,每站随机取样,共对3269个个体进行了基本生物学特性测定,研究了移植底播菲律宾蛤仔的生长、死亡等渔业生物学特性。结果表明,底播增殖菲律宾蛤仔苗种的平均个体质量0.26g、平均壳长11.1mm;底播蛤仔与野生蛤仔在生长规律上基本一致,春末至秋初(4~9月)是其主要生长期;1~3龄期间个体生长速度较快,1~2龄、2~3龄,个体质量分别增长3.88g和4.02g;1~2龄生物量增长最快,2~3龄由于死亡率增大,其生物量增长缓慢;1~3龄蛤仔软体部的生长速度快于贝壳,5~6月是菲律宾蛤仔的繁殖肥育期。根据个体生长特性,3龄为最佳采捕年龄;根据目前的养殖状况,2龄蛤仔已达到商品规格,从生物量上分析,采捕2龄蛤仔收益最高;6月是最佳的捕获时期。蛤仔生长具有明显的季节变化,水温是影响菲律宾蛤仔生长的主要环境因子。[中国水产科学,2006,13(4):642—649]  相似文献   

15.
In wild fish, gonad maturation and spawning induce modifications in feeding behaviour and dynamics of body stores which are possibly responsible for metabolic disorders. Conversely, food manipulations can modify reproduction of captive female broodstock. A restricted diet during early stages of the life cycle delays the first maturation age, a quantitatively restricted food supply during the stages of oocyte differentiation reduces the egg number, while a reduction of food levels during the last phases of oogenesis has only a small effect on egg size, composition and hatchability. During the spawning period, salmonid female broodstock seem to be more able to utilize carbohydrates than immature fish, they valorize the energy brought in by fats and have low quantitative requirements for proteins (28%). On the other hand, Red Sea bream broodstock have higher needs for proteins (45%).The incorporation into diet of liposoluble vitamins and essential fatty acids is necessary for normal reproduction, but (n-6) fatty acids seem to play a more important role than in juveniles. Although the effects and physiological role of carotenoids are not yet well known in salmonids, they are necessary for normal development in Red Sea bream. Although little work has been done on the effects of mineral nutrition, special attention must be paid to trace elements, manganese in particular.The major part of this review concerns female broodstock, since very little interest has been devoted to males.  相似文献   

16.
近年来,在野生渔业资源持续衰退、水产 养殖规模不断扩大、人们对鱼类福利关注度不断提升等的背景下,环境丰容作为一种全新的技术手段在水产领域受到广泛关注,被认为在野化放流鱼类行为、增加养殖鱼类产量、提升圈养鱼类福利等诸多方面均具有较大的应用潜力。环境丰容是指在增养殖生产中,采用适当方式向圈养或自然水体引入新的环境刺激,提高其异质性和复杂性,从而实现提升鱼类产量、提高鱼类福利、控制鱼类行为、改善鱼类生理目标的环境优化方式。总体来看,国际上围绕环境丰容技术的相关研究结果层出不穷,理论体系不断完善,但国内水产领域的相关研究尚处于起步阶段。本文在简要介绍环境丰容概念和分类基础上,聚焦目前最受关注的物理丰容方式,评述了物理丰容对鱼类打斗行为、生理应激、代谢生长等重要性状和放流后的适应性行为、个体适合度等增殖性状的影响,重点分析了引发研究结果差异的可能原因及其潜在神经可塑性机理,最后探讨了本领域以往研究的不足及今后的研究方向,旨在为我国开展该方面研究提供借鉴,为增养殖苗种高效健康培育与放流鱼类野化训练提供参考。  相似文献   

17.
Abstract. A quantitative gonadal index was developed for oysters, Crassostrea virginica (Gmelin. 1791), using polyclonal antibodies from eggs and sperm. Percoll used in the purification of oyster eggs and sperm greatly improved the purity of antigens compared to filtering the egg or sperm through a fine mesh only. The antigen-antibody reaction was tested with indirect sandwich ELISA using alkaline phosphatase-conjugated goat anti-rabbit igG as a secondary antibody. Rabbit anti-oyster egg IgG and anti-oyster sperm IgG initially exhibited a weak cross-reactivity over somatic tissue. Absoring with acetone-dried oyster tissue powder removed this cross-reactivity. Both antisera exhibited strong specific immunological reactions to oyster eggs or sperm respectively. The quantity of eggs or sperm was measured using ELISA and a quantitative gonadosomatic index (dry wt of egg or sperm/dry wt oyster) (GSI) calculated. GSI from ELISA correlated with gonadal stage measured histologically. Monthly mean GSI of female oysters was highest during late spring to early summer (0·157–0·201) and lowest during early winter to early spring (0·002-0·000). Maximum GSI observed during the study was 0·422 for female oysters and 0·446 for male oysters. Female oysters produce 3·7–65·4 million eggs, with an average of 21·1 million during each spawning. A positive correlation was observed between the number of eggs produced and oyster size; the number of eggs in the gonad increased as oyster size (i.e. total dry wt) increased (r= 0·67); however, the relationship was non-linear. Large oysters contained proportionally fewer eggs. Prevalence of Perkinsus marinus parasitism was high, 90–100%, during the study, as was weighted incidence, 1·33 to 2·67, No statistically significant correlation was observed between infection intensity and the per cent weight of oyster eggs or egg number.  相似文献   

18.
To determine the dynamics of the transmission of Aeromonas salmonicida ssp. salmonicida infection, chinook salmon, Oncorhynchus tshawytscha, were exposed to bacteria by cohabitation. The latent period (time between exposure and infectivity) was determined by exposing a group of chinook salmonid fingerlings to A. salmonicida by bath, then, at daily intervals, by holding five exposed (donor) fish with approximately 50 naive fish for 24 h. The latent period was 3 days post-infection and the time period between the initial exposure to bacteria and the beginning of bacterial shedding was 4.5 days for the same animals. The prevalence and intensity of infection in the donor fish, to which recipient fish were exposed, i.e. the level of exposure, was highly correlated with the development of disease in recipient (susceptible) chinook salmon (r2 = 0.57). An experiment was conducted to determine the daily progress of infection and development of a furunculosis epidemic among recipient fish by cohabiting a single exposed fish with 43 unexposed salmon. At daily intervals, all fish (in seven treatment tanks and one control tank daily) were sacrificed and tested for the presence of A. salmonicida in the kidney (n = 3520). Over 10 days, mean prevalence among recipient fish reached 75% and disease related mortality exceeded 50%. Bacterial concentrations in the water continued to increase over the duration of the experiment in concert with the number of infected animals present in the population.  相似文献   

19.
Over 130 cases of equine periodic ophthalmia (p.o.), which were treated as in-patients at the Equine Clinic of the Free University of Berlin in the last 35 years, were examined statistically in relation to the age and gender of the animals involved as well as to the development of the illness and the season in which it arose. As regards aetiology, the extraction of 71 affected Trotters was investigated. Antibodies to toxoplasmosis, leptospirosis and intestinal parasites were found only in some of the patients. Younger animals, aged between one and four years, and male animals (63.6%) were predominantly affected. The results of the examinations of faeces showed no correlation between cases of p.o. and a vermination of the horses. The examinations for antibodies to toxoplasmosis gave no indication of a participation of the toxoplasmas in the aetiology of recurrent uveitis. In contrast, the results of the examinations for antibodies to leptospirosis, of which 58.8% were positive, showed a seven to ten times higher infection than in healthy horses in Berlin. Breed analysis showed that in certain breeds of Trotters and warm-blooded horses, p.o. illnesses were frequent, supporting the hypothesis that the occurrence of p.o. is due to a hereditary, allergic reaction, triggered by various factors, in particular an infection without clinical signs.  相似文献   

20.
Mycobacterium spp. and Photobacterium damselae subsp. piscicida are recognized as the most frequent causative agents of granulomatous lesions in fish. Although frequent episodes of mycobacterial infections have been reported in wild fish worldwide, only sporadic cases have been documented to date in Italy. To investigate for the presence of lesions referable to mycobacteriosis and to identify the mycobacterial species involved, a total of 159 wild mullets were fished from the eastern coast of the Ligurian Sea, killed and necropsied. Liver and spleen samples were collected from all fish for histopathological and microbiological analyses. Molecular investigations for identification of Photobacterium damselae subsp. piscicida were performed. Gross examination revealed granulomatous lesions in one animal; microscopically, 42.14% of fish displayed granulomas with various histological features, 19.50% resulted positive at Ziehl–Neelsen staining, and were confirmed as mycobacterial lesions by culture. The identified colonies were characterized as M. fortuitum, M. abscessus, M. flavescens, M. chelonae, M. septicum and M. nonchromogenicum. In all, 35% of animals resulted positive for Photobacterium damselae subsp. piscicida. These data suggest widespread mycobacterial infection also by Photobacterium damselae subsp. piscicida infections in wild fish. Moreover, the pathogenicity of some mycobacterial species, previously considered as saprophytic, was demonstrated.  相似文献   

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