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1.
In the present study, attempt was made to compare agar with gum karaya as gelling agent in micropropagation of rough lemon (Citrus jambhiri Lush.). Initially nodal segments were cultured on agar-gel MS medium containing benzyladenine (BA), kinetin (KN), zeatin (ZN) (1.0–2.5 mg L?1) and malt extract (200–1,200 mg L?1) to standardize the medium. Maximum shoot regeneration (66.66%) was observed with KN 2 mg L?1 with an average shoot length of 0.73 cm. Gum karaya and agar was then evaluated at different concentration and combinations in same medium. The shoot regeneration response on media gelled with 30 g L?1 gum karaya was 62.49% with an average shoot length of 0.80 cm. Regenerated shoots were rooted on MS medium gelled with agar and supplemented with different concentrations (0.5–2.5 mg L?1) of indole-3-acetic acid (IAA), naphthalene acetic acid (NAA), and indole-3-butyric acid (IBA). Maximum response (52.77 %) was observed with IBA 2.0 mg L?1 with an average number of 2.58 roots/shoot. A maximum of 53.47% cultures showed root regeneration with an average number of 2.91 roots/shoot in 30 g L?1 gum karaya-gel medium. Texture measurements revealed that firmness of gum karaya-gel medium was nowhere near to that of agar. However, in their capability of supporting growth and differentiation of explants they are equal to agar medium. Gum karaya forms less adhesive and gummy medium as compared to agar. This study indicates that gum karaya can be used as gelling agent in place of agar.  相似文献   

2.
Potato leaf roll virus (PLRV) is causing serious loss in yield and quality of potatoes. In the present study, the effect of seven antiviral chemicals viz. Acyclovir, 5-Azacytidine, Cytarabine, 5-Bromouracil, Ribavirin, 2-Thiouracil and Zidovudine on regeneration response and production of PLRV-free plants under in vitro conditions is reported. MS medium supplemented with 0.1 mg L?1 GA3, 0.1 mg L?1 NAA and 500 mg L?1 malt extract was used for regeneration of plantlets from nodal explants. DAS-ELISA and RT-PCR was used for virus indexing of the mother plant and in vitro-regenerated plantlets. Explants of PLRV positive potato plants were cultured on this medium containing different concentrations (5 – 30 mg L?1) of antiviral chemicals. Shoot regeneration response varied between tested antiviral chemicals and was decreased with increase in concentration of antiviral chemicals from 5 to 30 mg L?1. Antiviral chemicals at 30 mg L?1 concentration showed strong inhibitory effect on regeneration response of shoots. In vitro regenerated plantlets tested negative in both ELISA and RT-PCR were only considered as virus free. When regeneration response and number of virus-free plants produced was compared, 2- thiouracil and ribavirin (25 mg L?1) were found to be effective. 2- thiouracil (25 mg L?1) gave 38.68% PLRV free plants with 30.55% cultures showing shoot regeneration and ribavirin (25 mg L?1) gave 39.62% PLRV-free plants with 36.80% cultures showing shoot regeneration. Regeneration response of explants was better on 5-Bromouracil at 30 mg L?1 concentrations but it was found least effective in production of PLRV-free potato plants.  相似文献   

3.
Kaempferia angustifolia is an aromatic, essential oil-yielding plant of the Zingiberaceae family with an ethno-medicinal repute. We standardized an effective system for micropropagation of K. angustifolia, and this is probably the very first report of in vitro culture of this species. Axillary buds were cultured on a Murashige and Skoog (MS) medium supplemented with various concentrations and combinations of plant growth regulators (PGRs) and spermidine. Highest multiplication occurred when the MS medium was supplemented with a combination of 2.0 mg L?1 6-benzylaminopurine (BAP), 2.0 mg L?1 kinetin (KIN) and 1.0 mg L?1 α-naphthalene acetic acid (NAA). Addition of spermidine (2.0 mM) along with optimum PGRs had further improved the multiplication rate with a maximum of 6.6 ± 0.36 shoots per explant within 60 days of implantation. The number of multiplied shoots per explant increased with each subsequent regeneration cycle; and the shoots per explant increased from 6.6 ± 0.36 on the 1st regeneration cycle to 10.3 ± 0.42 on the 2nd regeneration cycle and further increased to 13.7 ± 0.37 on the 3rd regeneration cycle on the same medium composition. The best result for in vitro root induction of multiplied shoot was achieved on a half-strength MS medium fortified with 2.0 mg L?1 IBA, with a maximum of 18.5 ± 0.28 roots per shoot. Regenerated plantlets were acclimatized with 88.9 % survival rate. After 9 months of field-transfer, all these plants were harvested and rhizomes were collected. However, the present protocol can definitely be applied for large-scale propagation and commercial cultivation of K. angustifolia.  相似文献   

4.
Factors affecting in vitro propagation and microtuberization were evaluated for Gloriosa superba L., an endangered ornamental cum medicinal plant having limited reproductive capacity. Surface sterilization of tuber explants with 0.1% mercuric chloride (HgCl2) for 5 min eliminated the contamination effectively with highest survival rate. Among the various combinations used, Murashige and Skoog (MS) medium with 2.0 mg L?1 6-benzylaminopurine (BAP) + 0.5 mg L?1 α-naphthalene acetic acid (NAA) containing 3% sucrose with 16-h photoperiod exhibited the greatest in vitro tuberization (3.2) with the highest shoot regeneration frequency (90%). The longest tuber regeneration occurred on MS media containing 4% sucrose. Transfer of in vitro-regenerated shoots to half-strength MS medium with 1.0 mg L?1 indole-3-butyric acid (IBA) + 0.5 mg L?1 NAA showed maximum root induction (66.6%). The in vitro-grown plantlets were successfully acclimatized and transplanted to sterilized soil and sand mixture (3:1) in the glasshouse with 70% survival. The colchicine content was determined in the tubers of ex vitro plants by HPLC using the same retention time (1.5 min) as that of the standard colchicine. This revealed that the micropropagation protocol developed by us for rapid mass production could be used as raw material for colchicine extraction and provides a basis for germplasm conservation and genetic improvement of G. superba.  相似文献   

5.
We established an advanced protocol for in vitro propagation of Aloe vera via comparison of basal media, sucrose contents, growth hormone combinations, and additional supplementation with various polyamines. The maximal number and growth of shoots after 5 weeks was obtained using MS media including 30 g L?1 sucrose supplemented with 1.0 mg L?1 BA and 0.1 mg L?1 NAA. To improve shoot production, various concentrations of putrescine, spermidine, and spermine were added under optimal growth hormone conditions (MS media supplemented with 30 g L?1 sucrose, 1.0 mg L?1 BA, and 0.1 mg L?1 NAA). Maximal shoot number and growth after 5 weeks were achieved with supplementation of 50 mg L?1 spermidine. Regenerated plants were successfully acclimatized in soil with 100% efficiency. Cytogenetic inspection revealed that the regenerated plants maintained intact chromosomes identical to those of plants grown in field conditions. This protocol provides a valuable alternative for mass production of elite Aloe vera.  相似文献   

6.
In the present study, in vitro propagation of tribal endemic medicinal plant, Andrographis lineata has been established using mature nodal explants. High frequency of regeneration (91.4%) was achieved on MS medium containing BA (3.0 mg L–1) along with IAA (0.2 mg L–1). Strikingly, irrespective of the season and collection period, we observed axillary flower induction and fruit formation from the above in vitro cultures supplemented with BA and NAA. Transition from the vegetative to the reproductive phase occurred within 2 months of culture, and importantly was influenced by factors such as sucrose and cytokinins. In vitro-regenerated flowers were morphologically identical to in vivo flowers. Furthermore, our scanning electron microscopic studies revealed that pollen external morphology of both in vitro and in vivo flowers were similar. The flowers self-fertilized and produced fruits in vitro. Elongated shoots rooted well on half-strength MS basal medium, and were successfully acclimatized to the garden conditions. Altogether, our established protocol can be utilized in plant breeding for the purpose of ex situ conservation, quick flowering, and fruit set.  相似文献   

7.
As a medicinal plant, the importance of evening primrose (Oenothera biennis L.) is due to its unsaturated fatty acids in the seeds and roots, and also oenotherine and comfarol in the leaves. Low germination and difficulties in seed production are the main problems encountered with growing this plant in the field. As an alternative approach, an in vitro experiment was set up for the evaluation of evening primrose production via direct and indirect regeneration of the cultivars NC-1 and VNK. For callogenesis and direct regeneration, the explants from the apical bud and petiole were cultured on MS medium supplemented with 0.25, 0.75, and 1.25 mg L?1 of both BAP and Kinetin (KIN). Indirect regeneration was performed by placing apical buds, petioles, and leaf explants on MS medium supplemented with 0.5 and 1 mg L?1 2,4-D and 0.5, 1, and 1.25 mg L?1 of both BAP and KIN. The highest shoot induction from direct regeneration was obtained with apical bud explants of VNK treated with 0.75 mg L?1 BAP. The highest callus weight (3.17 g) obtained from indirect regeneration was with petiole explants treated with 1 mg L?1 2, 4-D and 1 mg L?1 BAP in VNK cultivars. The highest number of torpedo embryogenic clusters (23.8) was obtained from the VNK petiole explants treated with 0.5 mg L?1 2, 4-D and 1.25 mg L?1 BAP. BAP had higher positive effects on in vitro production of evening primrose than KIN in both direct and indirect regeneration. In general, results indicated that VNK was more potent for regeneration than NC-1 and concentrations of 0.75 mg L?1 BAP for direct and 0.5 mg L?1 2, 4-D and 1.25 mg L?1of BAP for indirect regeneration had a higher efficiency for increasing in vitro production of evening primrose.  相似文献   

8.
The effect of plant growth regulators and natural supplements on the morphogenetic response of Pogostemon cablin Benth. was investigated. Murashige and Skoog (MS) media supplemented with 0.5 mg L?1 benzyl-6-adenine and 0.5 mg L?1 kinetin was effective in inducing multiple shoots (63.20 ± 0.15) with an average shoot length of 5.27 ± 0.15 cm and biomass of 5.20 ± 0.10 g shoot?1. Among the natural supplements, 10% coconut water supplemented to MS media showed a better response in all the morphological parameters studied. The use of 10% tomato extract, 20% banana extract, 10% carrot extract, and 10% papaya extract in MS medium have efficiently increased multiple shoots, shoot length, and fresh weight of the shoots. The natural supplements also effectively increased the chlorophyll content, total protein, and total carbohydrate content in the plant. The frequency of rooting (93%) was highest when shoots were implanted on 1/2 strength MS media with 100 mg L?1 activated charcoal. The in vitro rooted plants were successfully acclimatized and established in soil. Also, RAPD analysis showed no variation suggesting true-to-type nature of the micropropagated plants. Hence, this protocol can effectively reduce the cost of in vitro multiplication of plants.  相似文献   

9.
Although several studies have been made on the micropropagation of Jatropha curcas using agar base mediums, none of them have been by using liquid medium systems. The effects of explant type and temporary immersion system (test tube, jar with filter paper boat, and growtek bioreactor) on the micropropagation of J. curcas were studied. The explant type influenced shoot quality, multiplication coefficient (MC), and rooting. Leaf explant produced more and longer shoots than nodal explant. Use of filter paper (FB) boat prevented hyperhydricity and allowed proliferation of nodal explants cultured in liquid MS (Murashige and Skoog) medium supplemented 6-benzylaminopurine (BAP) and Kinetin (KN). The best shoot bud induction (92.1±3.1%) was achieved in liquid MS medium supplemented with 2.0 mg/L KN. Leaf regeneration efficiency was compared in growtek bioreactor and in jar containing liquid MS medium supplemented with 0.5 mg/L Thidiazuron (TDZ). The best shoot bud regeneration (78.7±2.1%) was obtained in growtek bioreactor. Shoot buds achieved from nodal segment and leaf were subcultured on filter paper boats in jar and bioreactor containing liquid MS medium supplemented with BAP, Indole butyric acid (IBA), Indole-3-acetic acid (IAA), and KN. Best shoot proliferation and elongation was obtained in filter paper boats containing liquid MS medium supplemented with 1.5 mg/L BAP, 0.5 mg/L IAA, and 0.2 mg/L KN. The number of multiple shoot buds was higher in leaf explants as compared to nodal explants and the highest number of multiple shoot buds was recorded from leaf explants. Up to 76.4% rooting efficiency was obtained when the shoots were ex vitro rooted. The generated plants well established in the nursery and grew normally in outdoor conditions. The protocol has good potential for application in large-scale propagation of J. curcas using liquid medium.  相似文献   

10.
The present study describes the procedure for micropropagation of Dracocephalum kotschyi L. using shoot tips from in vitro-germinated plants. The best response was observed for shoot tips on MS medium containing 5 mg 6-benzylaminopurine L?1 and 0.2 mg 1-naphthaleneacetic L?1 acid. Regeneration for other types of the explant hypocotyls and cotyledons did not show satisfactory results so that the explants did not develop into normal shoots and in turn developed into the calli after 12 days of culture. Histochemical analysis showed that only the shoot tip revealed a direct induction of more teratological protuberances that arise around the cut end of the explants. Elongation of shoot buds was obtained on MS medium containing 1 mg BAP L?1 + 0.5 mg IBA L?1. Regenerated shoots rooted best on the same medium of elongation. After hardening, the rooted plants were transferred to the greenhouse where they grew, matured, and flowered normally with a survival rate of 95%. We concluded that the present protocol can be efficiently used for mass propagation of Dracocephalum kotschyi.  相似文献   

11.
Jatropha curcas, the energy plant has attained great attention in recent years because of its biodiesel production potential; however, oil and deoiled cakes are toxic. A non-toxic variety of J. curcas is reported from Mexico. A simple and efficient protocol has been developed for plant regeneration using cotyledonary petiole explants of non-toxic variety of J. curcas. The percentage of induction of shoot buds (59.11%), and the number of shoot buds (5.01) per explant was achieved on Murashige and Skoog’s (MS) medium supplemented with 2.27 μM thidiazuron (TDZ). These induced shoot buds multiplied when subcultured on MS medium supplemented with 10 μM kinetin (Kn), 4.5 μM 6-benzyl aminopurine (BAP), and 5.5 μM α-naphthaleneacetic acid (NAA) for 4 weeks and subsequent elongation achieved on MS medium supplemented with 2.25 μM BAP and 8.5 μM indole-3-acetic acid (IAA). Shoots more than 2 cm long were harvested and cultured on MS medium containing different concentrations and combinations of IBA, IAA, NAA, and 0.25 mg L?1 activated charcoal, and 19.91% rooting was achieved in 15 μM IBA, 5.7 μM IAA, and 16.5 μM NAA after 4 weeks with more than 90% survival rate.  相似文献   

12.
The main objectives of this study were to investigate physicochemicals and antioxidant activities of new red rice (Oryza sativa cv. Gunganghongmi (GH)) by comparing normal brown (Nampyeongbyeo, NB) and reported red rice (Jukjinjubyeo, JB) in Korea. The nutritional constituents, including protein, oil, sugar, fatty acid, GABA, and γ-oryzanol were not significantly different between normal brown and colored rice. However, the ethanol extract of GH showed the highest phenolic content (24.7 ± 1.3 mg g?1). The ethanol extracts of GH showed higher scavenging activities against DPPH (0.2 mg mL?1 = 62.1 ± 2.5%) and ABTS (0.2 mg mL?1 = 63.2 ± 3.5%) radicals. Moreover, GH more inhibited LPS-induced nitric oxide (NO) production (13.2 ± 1.4 μM) than JB (18.3 ± 2.3 μM) and NB (22.1 ± 1.4 μM) at the same concentration (0.2 mg mL?1) without cytotoxicity. These results suggest that new red rice (GH) would be considered to be new functional rice due to its anti-oxidative effect and high nutrition.  相似文献   

13.
Mungbean (Vigna radiata) and rice bean (V. umbellata) (both species 2n = 2x = 22) have desirable traits that complement each other. In this study, we rescued embryos from a cross between mungbean cv. “Kamphaeng Saen 2” and rice bean cv. “Miyazaki” and resolved the hybrid sterility problem by colchicine treatment. The interspecific hybrids were obtained when Kamphaeng Saen 2 was used as the female parent. Four out of 80 immature seeds at 12 days old were able to germinate on an MS medium supplemented with 1 mg L?1 IAA, 0.2 mg L?1 kinetin, and 500 mg L?1 casein hydrolysate. Forty random amplified polymorphic DNA (RAPD) primers were screened for polymorphism among the parents, and two specific primers were finally chosen for testing of hybridity. Using the two primers, all putative F1 hybrids were confirmed as the interspecific hybrids. To observe their fertility, some of the hybrid seedlings were transplanted. The hybrid produced flowers profusely but failed to set pods. To overcome the sterility, plants were induced to become tetraploid by colchicine treatment in vitro. The ploidy level of the regenerated seedlings was confirmed from leaf DNA using a flow cytometer. Three out of 20 hybrid seedlings (15%) were successfully induced from diploid to tetraploid by a colchicine concentration of 2 g L?1. The tetraploid hybrids were able to produce flowers and set pods normally.  相似文献   

14.
An in vitro propagation method for female plants of Momordica dioica (Roxb.) has been established. The nodal segments were harvested and the cut ends of the explants were sealed with wax and then surface sterilized and cultured. Bud breaking occurred on Murashige and Skoog’s (MS) agar-gelled medium + 2.0 mg L−1 6-Benzylaminopurine (BAP) + 0.1 mg L−1 Indole-3 acetic acid (IAA). The cultures were amplified by passages on MS medium supplemented with 1.0 mg L−1 BAP + 0.1 mg L−1 IAA. Further, shoot amplification (29.2 shoots per vessel) was achieved by subculturing of in vitro regenerated shoot clump on MS medium + 0.5 mg L−1 BAP + 0.1 mg L−1 IAA. The micropropagated shoots were subsequently transferred for root formation on half-strength MS medium + 2.0 mg L−1 Indole-3 butyric acid (IBA) with 89% success rate. The in vitro-regenerated shoots were also rooted ex vitro with 34% success. These plantlets were hardened in the greenhouse and transferred to the field. The established protocol is suitable for true to type cloning of mature female plant of M. dioica.  相似文献   

15.
Due to its vegetative reproduction, saffron has a narrow genetic base and induced in vitro variations provide opportunities for expanding new cultivars. The objectives of this study were to evaluate sodium azide-induced variations in saffron’s corm culture in order to increase salt tolerance and pharmaceutical ingredients. Corm explants from the well-known ecotypes, Estahban and Kashmar, were subjected to various concentrations of sodium azide (NaN3) (0.09, 0.12, and 0.22 mg L?1) and NaCl (1.5, 2.5, and 4.0 dS equivalent to 0.07, 0.12, and 0.20 g NaCl in 100 mL water) in Murashige and Skoog medium supplemented with 1 mg L?1 2-4-D, 1 mg L?1 BAP, and 30 g L?1 sucrose and in a second pot culture experiment. The active pharmaceutical ingredients (crocin, picrocrocin, and safranal) were measured by high-performance liquid chromatography (HPLC). Variations in sodium azide-treated plants were more broadened for callus fresh weight (0.57–7.57 g), embryo weight (1.24–10.29 g), and regenerated seedlings (3.0–21.25) compared with those (0.12–3.77 g, 0.56–4.56 g, and 0.25–11.50, respectively) that were not treated with sodium azide. Under 0.20% salt, flowering failed in some of plants developed from sodium azide-untreated corms. HPLC analysis indicated wider ranges for crocin (11.92–18.03 mg g?1), picrocrocin (8.99–14.76 mg g?1), and safranal (2.13–7.36 mg g?1) in sodium azide-treated plants compared to the ranges (0.0–16.1, 0.0–12.5, and 0.0–6.66 mg g?1, respectively) in untreated plants. From a breeding perspective, induced variations found in this study would be useful to improve saffron’s quality and salt tolerance.  相似文献   

16.
Previously we reported that postproduction quality of pot ‘Seadov’ tulip (Tulipa gesneriana) was significantly increased by GA4+7 plus BA in a manner dependent on the concentration and stage of flower development at application. In these experiments, we extended the survey to 20 tulip cultivars to further evaluate the effects of GA4+7 plus BA sprays for enhancing postproduction flower and leaf quality. The senescence symptom of the cultivars fell into three categories: wilting, wilting-abscission (abscission shortly after tepal wilting) and abscission (abscission without wilting), with the majority of the cultivars belonging to the wilting and wilting-abscission categories. Pots bearing six plants were sprayed with a range of GA4+7 plus BA concentrations at marketable stage and placed in a simulated consumer environment (SCE). GA4+7 plus BA significantly enhanced individual flower and postproduction longevity, but the effect was dependent upon the senescence category of the cultivar. In general, GA4+7 plus BA increased individual flower and postproduction longevity of wilting-type cultivars at concentrations above 10 mg L?1, while longevity of wilting-abscission-type cultivars was only enhanced by 50 mg L?1. Abscission-type cultivars were not affected by any concentrations of GA4+7 plus BA. Regardless of floral senescence category, leaf yellowing was significantly reduced by GA4+7 plus BA sprays in those cultivars showing postproduction leaf yellowing. GA4+7 plus BA did not induce leaf and stem elongation in most cultivars. Only ‘Yellow Baby’, the shortest cultivar, showed elongation of stem and leaf by GA4+7 plus BA at concentrations above 25 mg L?1. Spray applications of GA4+7 plus BA can be useful to enhance flower and leaf quality in pot tulips.  相似文献   

17.
Crosses were made to produce interspecific hybrids between Brassica napus × B. juncea and their reciprocals with the aid of embryo culture techniques. A better response of hybrid embryo culture was obtained from two cross combinations of B. juncea × B. napus (Ames 24521 × Huyou 15 and Vittasso × Zheshuang 72) than from their reciprocals. Embryo culture was more effective in terms of plant regeneration when embryos were cultured in vitro at 15 days after pollination (DAP), while more calli were initiated when embryos were excised and cultured at 10 DAP. A better response was observed on the MS medium with 0.3 mg l?1 naphthylacetic acid (NAA) + 1.5 mg l?1 6‐benzylaminopurine (BAP) and with 0.3 mg l?1 NAA + 2.0 mg l?1 BAP. Callus formation and plant regeneration on these two media reached 55.43 and 26.65 %, and 66.98 and 24.61 %, respectively.  相似文献   

18.
Synthetic seed technology is a potential tool for a more efficient and cost effective rapid clonal propagation system. In the present investigation, synthetic seeds were produced by encapsulating nodal segments of Ocimum basilicum in calcium alginate gel. For encapsulation of nodal segment, 3% (w/v) sodium alginate and 75 mM CaCl2.2H2O were found most suitable. The synthetic seeds when cultured on half-strength Murashige and Skoog (MS) medium supplemented with 5.0 μM benzyladenine (BA) and 0.5 μM Indole -3- acetic acid (IAA) produced maximum number of shoots (7.9 ± 0.54) after 8 weeks of culture exhibiting 80% in vitro conversion response. Further, synthetic seeds stored at 4 °C for 4 weeks resulted in maximum conversion response (90%) when placed back to regeneration medium. Both root and shoot formation took place in the same medium but the roots were thin and difficult to handle. Individual elongated shoots were rooted on MS medium supplemented with 1.0 μM Indole -3- butyric acid (IBA). Plants regenerated from the synseeds were hardened, acclimatized, and established in soil with 80% survival rate. Changes in antioxidative enzymes viz., Superoxide dismutase (SOD) and Catalase (CAT) in O. basilicum indicated the adaptation of micropropagated plants to ex vitro conditions.  相似文献   

19.
二乔刺槐愈伤组织诱导及植株再生研究   总被引：2，自引：1，他引：1  
以二乔刺槐的幼嫩茎段为外植体进行愈伤组织诱导及植株再生研究。结果表明：二乔刺槐茎段诱导愈伤组织的最适培养基为MS+IBA0.2mg/L+BA3.0mg/L,愈伤组织芽诱导的最适培养基为MS+IBA 0.1 mg/L+BA 5.0 mg/L,根诱导培养基为1/2MS+ IBA 0.4mg/L。  相似文献   

20.
选择不同基因型的花生品种为外植体供体,以初步建立适应河南花生品种的高效再生体系。以5天苗龄的花生无菌胚轴为外植体,将供试的4个花生品种分别接种于4种丛生芽诱导培养基上：MS+6-BA5mg/L+NAA1mg/L,MS+TDZ0.6mg/L+NAA0.4mg/L,MS+TDZ1mg/L+6-BA1mg/L+NAA0.5mg/L,MS+TDZ1mg/L+6-BA2mg/L+NAA0.5mg/L。在25℃±1℃、2000lx、16h/d光照条件下培养约30天左右,上胚轴和下胚轴均分化出愈伤组织和丛生芽点。结果发现,上胚轴的丛生芽诱导率远高于下胚轴,最高达到67%,平均每个外植体产生4.5个丛生芽,最高的可分化出30多个;上胚轴在培养基MS+6-BA5mg/L+NAA1mg/L和MS+TDZ1mg/L+6-BA1mg/L+NAA0.5mg/L的丛生芽分化较好,该研究为花生组织的离体培养和外植体遗传转化提供有效途径。  相似文献   

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