共查询到20条相似文献，搜索用时 187 毫秒
1.
用蚕沙制取叶绿素铜钠盐邢萱（重庆工业管理学院）叶绿素是一种夭然色素，它不仅在医药、食品和日用工业中有着广泛用途，由它制得的叶绿素铜钠盐，更是生产治疗肝炎、胃及十二指肠溃疡药物的重要原料。蚕儿排出的蚕沙（即粪便）中，含叶绿素高达０．８～１．０％（干物）... 相似文献
2.
3.
一、蚕沙(包括蚕粪及残桑)是制取叶绿素及叶绿素铜钠盐的新原料,通过综合利用还可以制成其他产品,较用作饲料及肥料的经济价值提高30倍。二、蚕沙的色素与桑叶相似,经纸上层析法分离得6种色素,它们的顺序由下而上是:叶绿素b、叶绿素a,叶黄素。三色堇黄素、叶黄氧茂素及β葫蘿蔔素,是测定它们的Rf值。三、用光电比色法及分光光度法测定蚕沙及桑叶的叶绿素含量;蚕沙(干物)合叶绿素1.1205—1.2640％,桑叶合叶绿素1.0640—1.1375％,以春季的桑叶及蚕沙的叶绿素较多。四、蚕沙叶绿素制成水溶性叶绿素铜钠盐质量合符规格,纯度88—82％,相对消光度为3.4—4,得率0.8—2.05％。五、蚕沙叶绿素的吸收光譜与桑叶相似,而叶绿素纳盐的吸收光带偏于两极,卽6800—6500A°及4760—4000A°之间,蚕沙叶绿素的波长—消光曲线有两个吸收极大,叶绿素铜纳盐的波长—消光曲线的吸收极大为4070A°及6330A° 相似文献
4.
1959—1961年间应用蚕沙(粪)作原料,制取叶绿素铜钠盐,供作治疗非黄疸型传染性肝炎的临床试验,获得良好的效果。作者认为蚕沙是制取叶绿素及胡萝卜素的优良原料,并对其制取方法作了初步的探讨。 相似文献
5.
蚕沙叶绿素铜钠盐生产废液中β-胡萝卜素的大孔吸附树脂分离方法 总被引：2，自引：0，他引：2
研究了从蚕沙的叶绿素铜钠盐生产废液中制备β-胡萝卜素的色谱分离方法。采用高效液相色谱法(HPLC)考察了HZ816、DK110、D301和S8等4种树脂对叶绿素铜钠盐生产废液中β-胡萝卜素组分的静态吸附行为,确定了树脂极性和孔径大小等因素对β-胡萝卜素静态吸附量的影响。以HZ816树脂为固定相、乙醇和乙醚为洗脱液进行了原料液的动态吸附试验,发现用乙醇作溶剂上样分离时,叶绿素铜钠盐生产废液中的β-胡萝卜素组分被富集在固定相上,换用乙醚洗脱后该组分被置换出制备柱,从而获得β-胡萝卜素质量分数0.69%、收率97%的产品。 相似文献
6.
探讨用不同有效活菌数、纤维酶活含量的发酵菌种对蚕沙发酵腐熟及其中常见家蚕病原微生物灭活作用，研究蚕沙含水率对蚕沙发酵的影响。结果表明：每100kg蚕沙中有效菌数总量不低于500亿个，纤维素酶活总量不低于2500ug的发酵菌种，并且对蚕沙堆放盖膜处理，保证蚕沙的含水率不低于60％，才能够在短时间内让蚕沙迅速升温发酵，发酵温度达到60℃以上且持续7d以上，15d内蚕沙充分发酵腐烂降解，有效缩短堆肥时间。腐熟后的蚕沙全N为2．83％、全P20，为1．83％、全K20为4．4％、有机质含量67．88％，碳含量为26．25％，碳氮比（C／N）9．27，均能达到堆肥配比的要求，不需添加任何配料可直接回田使用，达到对蚕沙无害化处理的目的。 相似文献
7.
采用有机溶剂萃取法从松针中提取叶绿素,再制备成叶绿素铜钠盐,利用紫外吸收光谱测定其对光、热、酸碱以及氧化剂、还原剂的稳定性,为叶绿素铜钠盐的广泛应用提供技术参考。用松针制备叶绿素铜钠盐的紫外吸收光谱与标准曲线一致,亦与用蚕沙制备叶绿素铜钠盐的最大吸收峰位置一致。稳定性实验表明,用松针制备的叶绿素铜钠盐在<80℃的温度条件下很稳定,在中性和碱性条件下也较稳定,并具有很好的耐还原性,但其耐光性、耐酸性和耐氧化性稍差。据此建议叶绿素铜钠盐的保存温度应当控制在80℃以下,在使用时需尽量避免接触氧化剂,以及在pH 6～12的微酸、中性或碱性条件下使用。 相似文献
8.
以有效微生物群为生物发酵剂添加到蚕沙中进行堆肥发酵,调查蚕沙中家蚕病原灭活效果和蚕沙腐烂程度.结果表明：有效微生物群可促使蚕沙堆肥快速升温,对蚕沙中家蚕病原有较好的灭活效果,升温效果以添加0.1％EM原露溶液和0.1％VT-1000微生物发酵剂溶液架空并覆盖稻草为最佳,对家蚕核型多角体病、质型多角体病、白僵病的病原灭活率均达100％;堆肥时蚕沙中添加0.1％有效微生物群剂,第2天温度迅速升高,第3天温度达到顶峰,发酵高温期≥50℃的超过15d,≥60℃的12d,≥70℃的8d（最高温达82℃）.添加有效微生物、架空表面覆盖稻草比不添加有效微生物、不架空表面不覆盖稻草,发酵高温期（≥60℃）温度高1.9～10.2℃（平均为6.6℃）;而在不架空厌氧状态下添加有效微生物发酵剂堆肥中水分充足,在短时间内（14 d）蚕沙腐烂程度较好. 相似文献
9.
10.
11.
试验旨在研究不同水平蚕沙对新西兰兔肉品质的影响。选取120只健康、体重相近(1.06 kg±0.09 kg,公、母各半)的35日龄断奶新西兰兔,随机分为5组,每组6个重复,每个重复4只。对照组饲喂基础日粮,试验组分别饲喂添加6%、12%、18%和24%蚕沙的日粮。试验结束后,每组选取接近平均体重的4只兔(公、母各半)屠宰,测定肌肉品质指标。结果显示,不同水平蚕沙对新西兰兔肉品质有一定影响,其中各组肉色、pH、失水率和蒸煮率差异不显著(P＞0.05),而剪切值试验组显著低于对照组(P＜0.05),且随蚕沙添加量的增加呈减小的趋势;在化学性状中各组水分、粗蛋白质、粗脂肪、灰分含量差异不显著(P＞0.05)。 相似文献
12.
家蚕自古为滋补食疗珍品,丰富的有效活性成分使其具有独特的药理作用,为推进家蚕幼虫体的药用开发,用紫外分光光度法研究了家蚕幼虫体中黄酮类化合物含量在不同品种间的变化规律。将家蚕幼虫干燥粉碎,选用80%乙醇为溶剂,原料体积质量按0.0333g/mL在60℃下抽提4h,重复抽提3次,可高效地提取家蚕幼虫体黄酮类化合物。以NaNO2、Al(NO3)3显色,用紫外分光光度计于510nm波长下比色,可精确、稳定地测定出家蚕幼虫体中黄酮类化合物含量。结果表明,家蚕幼虫体富含黄酮类化合物,不同蚕品种其含量存在显著差异。 相似文献
13.
A HPLC-FLD method was developed for determination of enrofloxacin (ENR) and ciprofloxacin (CIP) levels in feces and urine of pig.The pig feces was ultrasonic extracted by acetonitrile-ammonia, then added trichloroacetic acid to make the extraction acidification.The pig urine was acidulated by phosphoric acid and the extraction of feces solution were enriched and purified by solid phase extraction small column, took purification liquid for HPLC analysis.Conditions of HPLC mobile phase was acetonitrile (A):citric acid/ammonium acetate buffer (B), the procedure of gradient elution was 0 to 25 min, A:10% to 40%;25 to 30 min, A:40% to 10%.The detector of fluorescence excitation wavelength was 278 nm, emission wavelength was 465 nm, chromatographic data were measured and recorded.The results showed that the LOD of ENR and CIP were lower than 0.01 mg/L in urine and 0.021 mg/kg in feces, the LOQ of ENR and CIP were lower than 0.03 mg/L in urine and 0.056 mg/kg in feces.ENR and CIP in the concentration of 0.01 to 1.0 mg/mL levels range had good linear relationship, R2 were 0.9994 and 0.9992 in pig urine, respectively;ENR and CIP in the concentration of 0.02 to 2.0 mg/mL levels range had good linear relationship, R2 were 0.9986 and 0.9981 in pig feces, respectively.The recovery ratio of ENR were 79.4% and 88.5%, and the recovery ratio of CIP were 75.8% and 89.9% in pig feces and urine.After get on validation, the method was easy in sample processing and testing, the results were accurate, reliable and high sensitivity, which was a worth promoting detection method. 相似文献
14.
为了对猪排泄物中恩诺沙星(enrofloxacin,ENR)和环丙沙星(ciprofloxacin,CIP)进行定量检测,试验建立了测定猪粪尿中ENR和CIP含量的高效液相－荧光检测方法。将猪粪经乙腈－氨水超声提取后,加入三氯乙酸酸化,然后分别将经磷酸酸化后的猪尿和提取后的猪粪溶液经固相萃取小柱富集净化,取净化液进行HPLC分析。HPLC流动相为乙腈(A):柠檬酸/乙酸铵缓冲液(B),梯度洗脱:0~25 min,A 10%~40%;25~30 min,A 40%至10%,荧光检测器的激发波长278 nm,发射波长465 nm。结果表明,ENR和CIP 在尿中的最低检测限(LOD)<0.01 mg/L,在粪中的LOD<0.021 mg/kg,在尿中的最低检测限(LOQ)<0.03 mg/L,在粪中LOQ<0.056 mg/kg,猪尿中的ENR和CIP在0.01~1.0 mg/mL范围内线性关系良好,R2分别为0.9994和0.9992;猪粪中的ENR和CIP在0.02~2.0 mg/mL范围内线性关系良好,R2分别为0.9986和0.9981。ENR在猪粪和猪尿中的回收率分别为79.4%和88.5%,CIP在猪粪和猪尿中的回收率分别为75.8%和89.9%。该方法样品处理简单,检测结果准确可靠,且灵敏度较高,是值得推广的检测方法。 相似文献
15.
Stephen H Stauffer Adam J Birkenheuer Michael G Levy Henry Marr Jody L Gookin 《Journal of veterinary diagnostic investigation》2008,20(5):639-641
Feces are increasingly valued as practical samples for molecular diagnosis of infectious disease. However, extraction of polymerase chain reaction (PCR) quality DNA from fecal samples can be challenging because of coextraction of PCR inhibitors. Because the type and quantity of PCR inhibitors is influenced by diet, endogenous flora, and concurrent disease, it is unlikely that extraction method performance with human feces can be directly extrapolated to that of domestic cats. In the present study, 4 commercially available DNA extraction methods were examined for their influence on the sensitivity of PCR for the detection of Tritrichomonas foetus in feline stool. DNA was extracted from serially diluted feline-origin T. foetus trophozoites in the absence or presence of feline feces. The ZR Fecal DNA kit was identified as affording the greatest analytical sensitivity and reproducibility and was able to detect >/=10 T. foetus organisms per 100 mg feces in 100% of PCR reactions. Further, the identified extraction method could be completed in the shortest time of all kits tested. 相似文献
16.
Jane Christopher-Hennings Matthew A Dammen Shelleen R Weeks William B Epperson Shri N Singh Gina L Steinlicht Ying Fang Jessica L Skaare Jill L Larsen Janet B Payeur Eric A Nelson 《Journal of veterinary diagnostic investigation》2003,15(2):87-93
In this study, 5 combinations of 2 DNA extractions and 3 polymerase chain reaction (PCR) techniques were compared with culture for the detection of Mycobacterium paratuberculosis directly from bovine feces. These combinations included a new commercial extraction technique combined with a commercial PCR/Southern blot technique, nested PCR (nPCR), or real-time PCR, and a university-developed extraction combined with nPCR or real-time PCR. Four of the 5 combinations had statistically similar sensitivities between 93% and 100% and specificity between 95% and 100%, when compared with culture results from 63 bovine fecal samples. These results indicated that using a commercial extraction with a commercial PCR/Southern blot, nPCR, or real-time PCR, or a university-developed extraction with real-time PCR would result in similar sensitivities to culture for the identification of M. paratuberculosis from bovine feces and are valid alternatives to culture. 相似文献
17.
探讨了存在于泥土地中的家蚕中肠型脓病病原,在室内自然环境中越冬后,对蚕的致病力显著降低.收集经越冬的病原添食感染后无病征出现的五龄蚕后期蚕粪,应用生测法检查有病原性,应用C.B.B法检查有多角体(CPB)存在.农村蚕室调查也表明.春蚕期虽未发现中肠型脓病,但在中秋蚕期,仍有该病出现.可以认为,多数是由于春蚕带毒蚕粪污染环境成为下一蚕期发病的感染源,并提出相应的预防对策. 相似文献
18.
蚕蛹壳聚糖的制备及应用研究进展 总被引：1，自引：0，他引：1
壳聚糖是甲壳素的脱乙酰化产物,具有安全无毒、抗菌、吸湿透气、吸附性强、成纤性好以及良好的化学反应活性和生物活性等特点。蚕蛹壳中含有丰富的甲壳素,是提取壳聚糖的优质原料。介绍了蚕蛹壳聚糖的制备工艺技术,着重讨论了蚕蛹原料预处理、脱无机盐、脱蛋白质、脱色、脱乙酰等工艺流程采用的技术方法;简述了蚕蛹壳聚糖在农业、纺织、食品加工、医用材料、环保、生物工程等领域的应用状况;分析了蚕蛹壳聚糖产业化生产存在的问题,提出了解决办法。 相似文献
19.
20.
Yanai Y Yamashiro H Isobe N Maeda T 《The Journal of reproduction and development》2007,53(5):1107-1112
The aim of this study was to evaluate whether the fecal progestagen (progesterone and its metabolites) levels of miniature pigs would change after excretion at room temperature. Our initial investigation focused on the correlations between the fecal progestagen concentrations with and without ether extraction and between the plasma progesterone and fecal progestagen concentrations in order to develop an enzyme-linked immunosorbent assay (ELISA) for fecal progestagen without ether extraction. There were significant correlations between fecal progestagen concentrations with and without ether extraction (r=0.880) and between fecal progestagen concentrations without ether extraction and plasma progesterone (r=0.763). The fecal progestagen concentration obtained by ELISA without ether extraction was almost identical to that obtained with ether extraction. These results validate the ELISA method without ether extraction, which was therefore used for the latter experiment. Fecal samples collected from the pigs were preserved for 0-24 h at room temperature, and then their fecal progestagen concentrations were measured. The fecal samples preserved for 0 to 24 h were analyzed by high performance liquid chromatography (HPLC) and ELISA. The concentrations of all samples significantly increased with time after preservation. The progestagen concentration of fresh feces (0 h) with high progestagen concentration (>1000 ng/g) increased significantly after 3 h. The concentration increased significantly after 12 h for fresh feces containing about 500 ng/g progestagen. HPLC analysis is showed that the fecal progesterone concentration, but not its other metabolites, doubled 24 h after excretion compared with the concentration at 0 h. These results suggest that dynamic changes in the profile of progesterone metabolites occur in feces after excretion. 相似文献