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在含有1毫克/升吲哚乙酸和2毫克/升6-苄基腺嘌呤(BA)的MS-Nitsch培养基上,从“苏母娜”葡萄(Vitis vinifera cv.Sultana)的分生组织培养中得到了强壮的组织和器官分化。不加6-苄基腺嘌呤,以萘乙酸替代吲哚乙酸同时添加0.1毫克/升赤霉酸诱导了愈合)组织)。然而,这种愈合组织被转移到含有吲哚乙酸和6-苄基腺嘌呤的新的培养基之后不能产生茎芽。为了使分生组织培养的茎芽诱导生根,采用一种内含1毫克/升吲哚乙酸,0.2毫克/升6-苄基腺嘌呤和10毫克/升盐酸半胱氨酸的培养基最为合适,同时为了小植株的不断产生,激素含量逐渐降低的培养基值得推荐。 相似文献
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牡丹试管苗生根过程解剖结构观察及相关激素与酶变化的研究 总被引：5，自引：1，他引：4
以‘太平红’牡丹试管苗为材料,对其生根诱导过程中吲哚乙酸（IAA）、细胞分裂素（ZR）和脱落酸（ABA）含量,过氧化物酶（POD）、多酚氧化酶（PPO）和吲哚乙酸氧化酶（IAAO）活性以及根形态结构进行了研究。结果表明：试管苗不定根的根原基发生于形成层。根原基发生期为诱导生根3 d左右,15 d时不定根突破表皮。内源激素在根原基诱导期间整体呈下降趋势,而在不定根的伸长期整体呈上升趋势。POD活性在根原基发生期间呈下降趋势,在不定根的伸长期间波动较大;PPO和IAAO活性在根原基的诱导期间呈上升趋势,在不定根的伸长期间PPO活性先下降后上升,IAAO活性呈下降趋势。 相似文献
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近年来,我们观测了萘乙酸(NAA)、吲哚乙酸(IAA)、吲哚丁酸(IBA)及赤霉素(GA)等生长激素,对平菇菌丝、子实体生长和品质的影响。现整理如下,供有关方面参考。 (一)对菌丝体生长的影响:在PDA培养基中,2ppm的萘乙酸和吲哚丁酸可明显促进菌丝生长,增长率达5～7％,而吲哚乙酸和赤霉素有一定的抑制作用。大于2ppm吲哚乙酸的抑制作用最强。在木屑培养基中,1ppm的各种生长激素都能不同程度地促进菌丝生长,5ppm的萘乙酸和吲哚丁酸效果最好,菌丝率增长可达7～10％。大于1ppm的吲哚乙酸和赤霉素有抑制作用。木屑培养 相似文献
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<正>1促进扦插生根吲哚乙酸、吲哚丁酸、萘乙酸、2,4-D均可促进大白菜、甘蓝、西瓜、甜瓜的扦插生根,其中以吲哚乙酸效果最好,仅将插条底部浸沾药液即可。番茄插条则用萘乙酸或吲哚乙酸浸泡10分钟左右。 相似文献
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1 试验目的扦插是葡萄繁殖育苗的主要方法 ,插条进行催根处理可以提高成苗率 ,催根的方法有多种 ,通过几种生长调节剂对比试验 ,寻找适合本地环境条件和主栽品种的制剂及浓度、时间等。2 试验方法2 1 试验材料 ①吲哚丁酸 (IBA) ;②吲哚乙酸(IAA ) ;③萘乙酸 (NAA ) ;④ABT生根粉 2号(ABT2 ) ,插条为充实老熟的巨峰枝条。2 2 处理 ①吲哚丁酸浓度为 :a 5 0× 10 - 6 ,b 80× 10 - 6 ,c 10 0× 10 - 6 ,浸 2 0h。②吲哚乙酸浓度为 :a 5 0× 10 - 6 ,b 80× 10 - 6 ,c 10 0× 10 - 6 ,浸 2 0h。③萘乙酸浓度为 :a 5 0× 10 - 6 … 相似文献
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葡萄扦插育苗技术研究 总被引：1，自引：0，他引：1
采用ABT生根粉、吲哚丁酸、吲哚乙酸、萘乙酸等药剂处理葡萄插条,结果表明,各种药剂对插条生根都有一定的促进效果,以50mg/L吲哚丁酸处理的效果最佳,成活率为93%,明显高于对照（清水）;其次150mg/L吲哚丁酸、200mg/L萘乙酸和50mg/L吲哚乙酸处理,成活率分别为90%、87%和83%。新梢平均长度,各处理均高于对照;单株平均生根数各处理均多于对照;发根平均长度,各处理均短于对照。 相似文献
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光照强度对郁金香生长发育和内源激素含量的影响 总被引：1，自引：0，他引：1
通过对郁金香进行遮荫处理,研究全光照(CK)、55%～60%光照(B1)、25%～30%光照(B2)、10%～15%光照(B3)4种不同光照强度对郁金香生长和内源激素的影响。结果表明:遮荫提高了郁金香在自身支撑结构的投入,株高变大,叶片变长,叶面积增大,过度遮荫抑制了有性生殖,表现为开花率降低,花径变小。随着光照强度的降低,郁金香叶片内吲哚乙酸含量和赤霉素含量升高,而脱落酸含量降低,遮荫降低了ABA/IAA与ABA/GA3比值,过低比值的ABA/IAA与ABA/GA3不利于成花。 相似文献
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‘新高’梨雄性不育与IAA和ABA含量变化的关系 总被引：6，自引：0，他引：6
以雄性不育的‘新高’梨和可育的‘丰水’梨为试材, 研究花粉发育过程中IAA和ABA含量及其与IAA代谢相关酶和微量元素的变化。结果表明: 丰水花蕾中IAA含量从四分体后极显著地高于新高, 在新高花粉败育期(单核花粉早期到单核花粉晚期) 是新高的3.70～4.47倍, 新高IAA含量的亏缺可能是导致败育的原因之一; 新高在花粉发育前期IAA氧化酶活性显著高于丰水, 而两个品种POD活性变化一致, 差异不显著, 说明POD不是导致新高IAA含量的亏缺的关键酶; 新高Zn含量低于丰水, 而Mn含量从单核花粉期以后却高于丰水; 新高ABA含量从四分体到二核花粉期显著高于丰水, 而丰水在成熟的花药中积累了大量的ABA。 相似文献
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研究了高效液相色谱法测定香蕉根中4种内源激素:赤霉素(GA3)、3-吲哚乙酸(IAA)、脱落酸(ABA)和玉米素(ZT)的最佳条件。采用Agilent ZORBAX SB-C18柱(250mm×4.6mm,5μm)进行分离测定。结果表明:GA3、IAA、ABA测定的最佳条件是甲醇∶乙腈∶磷酸缓冲液(pH 3.5)为15∶20∶65为流动相,检测波长为210nm。ZT测定最佳条件为:甲醇∶乙腈∶磷酸缓冲液(pH 3.5)为15∶15∶70为流动相,波长265nm。4种激素的进样量为20μL,流速为1mL/min,柱温为35℃。选用外标法进行了定量测定。试验结果表明其回收率高,是一种快速有效的测定方法。 相似文献
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‘St. Julien A’ (Prunus instititia L.) rootstock was induced to proliferate shoots on a modified half-strength Murashige and Skoog (MS) medium. Cultures treated with 12.5 mg l?1 gibberellic acid (GA3) produced elongated shoots suitable for rooting. Elongated shoots were placed in media with indolebutyric acid (IBA) or indole-3-acetic acid (IAA) with or without a 16-day dark incubation. Light (16-h photoperiod) inhibited rooting. IAA (4 mg l?1) was ineffective in promoting rooting. Rooting was best when shoots were incubated in the dark with IBA (4 mg l?1). GA3 was deleterious to shoots, causing chlorosis and apical die-back. Light regime interacted with auxin treatments in affecting shoot condition. Shoot condition was better on shoots treated with IBA and dark-incubated; while those treated with IAA were better when light-incubated. 相似文献
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Expression,subcellular localization and phytohormone stimulation of a functional sucrose transporter (MdSUT1) in apple fruit 总被引：1，自引：0，他引：1
Sucrose transporters or carriers (SUTs or SUCs) play a crucial role in the cell-to-cell distribution of sucrose throughout the plant. Our data demonstrated that MdSUT1 is expressed almost ubiquitously in leaves, shoots, flowers and fruits, and the MdSUT1 was localized in plasma membranes of both sieve element/companion cell complex and storage parenchyma cells in apple fruits detected by immuno-gold labeling. The MdSUT1 expression was rapidly up-regulated by abscisic acid (ABA) at the translational levels in the treatments of the fruit tissues. Ten μmol L−1 ABA and gibberellin acid (GA) induced MdSUT1 protein amounts dramatically, whereas 6-benzyl aminopurine (6-BA), indole-3-acetic acid (IAA) and cis-(−) ABA showed no distinct induction effect. These results suggest that MdSUT1 may be a component of ABA signaling pathways involved in the regulation of photoassimilate transport. ABA could function in plant sucrose trans-membrane transport, thus providing a clue that may help to unravel cross-talk between plant hormone and sucrose signaling pathway. 相似文献
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The present study was carried out to assess the effect of explant preparation and sizing for in vitro micropropagation of Aloe vera L. The stem nodal explants and shoot tips were cultured on modified Murashige and Skoog's medium (1962) supplemented with different concentrations of 6-benzylaminopurine (BA), kinetin (KIN), indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) and α-naphthaleneacetic acid (NAA) either singly or in combination. The best media composition was found to be MS medium supplemented with IAA (11.42 μM), IBA (9.8 μM) and BA (8.88 μM). The explants were divided into 2 sets, with and without ensheathing leaf base. Explant sizing, pruning and retention of mother tissue was highly significant in induction of multiple shoots and roots. The stem nodal explants with leaf base performed much better than those without such covering. A very high number of shoots and roots grew from these explants. The rooted plantlets were successfully acclimatized and transferred to the green house conditions and finally to field conditions. 相似文献
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《Scientia Horticulturae》2005,105(4):475-482
The present study was conducted to evaluate the regeneration ability of Damask rose. Single-node explants were surface sterilised with 10% chlorox for 15 min and cultured on Murashige and Skoog (MS) medium supplemented with various concentrations of N6-benzyladenine (BA) or kinetin (Kin) separately or in combination with different concentrations of indole-3-butyric acid (IBA). Combination of 2.5–3 mg/l BA and 0.1 mg/l IBA was the most suitable treatment for proliferation. In vitro derived shoots were subcultured four times on the fresh medium within a 4-week period. Other treatments such as explant orientation (horizontal, vertical and oblique) and explant wounding were also examined but did not affect shoot multiplication rate significantly. Several experiments were carried out to stimulate in vitro rooting of Damask rose. Application of different media such as MS, 1/2 MS, 1/3 MS and 1/4 MS with different concentrations of indole-3-acetic acid (IAA), IBA and naphthaleneacetic acid (NAA) did not produce satisfactory results. Quick-dip method using sterilised 0–2000 mg/l IAA, IBA and NAA solutions was also studied. Other treatments such as using various concentrations of abscisic acid (ABA) in combination with various concentrations of IAA, IBA and NAA, and using various concentrations of sucrose and agar did not produce any roots. The best treatment for rooting of shoots was 2.5 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) for 2 weeks in MS medium and then transferring the explants to MS medium without any growth regulator. Plantlets were acclimatised in a soil mixture consisting of peat moss and sand 1:1 (v/v) and successfully transferred to the greenhouse after 3 weeks. 相似文献
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以山葡萄雄株为试材,花前20d用100mg/L的KT-30S处理雄株花序,以蒸馏水处理为对照,观察处理后的形态变化和坐果情况,并在不同处理时期取样测定内源激素含量,探讨外源细胞分裂素KT-30S对山葡萄雄株花蕾性别转换的效果及其对内源激素的影响。结果表明:KT-30S对山葡萄雄株的性反转效果显著,雄花花蕾在处理6d子房显著膨大。经KT-30S处理,4种内源激素含量和平衡关系表现出不同的变化趋势,由此推断,KT-30S诱导山葡萄雄株性反转可能是通过调节内源激素在不同时期的水平及其之间的平衡关系来实现的。 相似文献
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I. C. Karapanos C. Fasseas C. M. Olympios 《The Journal of Horticultural Science and Biotechnology》2013,88(4):631-638
SummaryThe present work examines the effects of substrate additions and incubation conditions on in vitro germination of tomato pollen on semi-solid, agar-based substrates with the ultimate aim of defining a standard semi-solid substrate for tomato pollen germination studies. Partial replacement of sucrose by polyethylene glycol (PEG-6000) for osmotic regulation of the substrate significantly increased pollen germination of both the low temperature-susceptible F1 cultivar ‘Dombito’, and the more temperature-resistant cv.‘Supermarmande’, to a level that varied according to the season of pollen harvest. In contrast, partial substitution of sucrose by mannitol was inhibitory to an extent that depended on the final concentration of mannitol in the medium. The optimum pH for germination was 6.5 and the optimum incubation temperature was 15°C. Among the vitamins (riboflavin, thiamine, pyridoxine, niacin, pantothenic acid, p-aminobenzoic acid), amino acids (glutamic acid, aspartic acid), casein hydrolysate, plant growth regulators (gibberellic acid, indole-3-acetic acid, indole-3-butyric acid, indole-3-propionic acid, 1-naphthaleneacetic acid, kinetin) and flavonoids (naringin, myricetin, naringenin, quercetin, rutin) tested, a significant increase in pollen germination was induced only by the flavonoids quercetin and myricetin and, to a lesser extent, by indole-3-acetic acid. In contrast, both kinetin and naringenin were inhibitory, as were both amino acids at 100 mg l–1 and casein hydrolysate (at 1.0 – 10.0 g l–1). On the basis of the above results, we conclude that, for both tomato cultivars tested, the most suitable semi-solid substrate among those examined was that containing 10% (w/v) sucrose, 15.1% (w/v) PEG-6000 and 1.5% (w/v) agar, with the possible addition of 5 mg l–1 quercetin, or 5 mg l–1 myricetin. 相似文献