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以氮、碳离子注入甜菊种子研究其电导率、ｐＨ值及发芽能力的变化，结果表明，被处理种子的电导卓，随着浸种时间的延长而提高，以注入能量７５ｋｅＶ与剂量１０１４最为敏感，氮离子比碳离子对电导率的影响作用更大；离子注入甜菊种子后，ｐＨ值均在４．５～５．０之间，碳离子稍高于氮离子，能量与剂量的变化反应不敏感；离子注入后甜菊种子的发芽势与发芽率有相同趋势，小剂量、低能量促进种子发芽，碳离子比氦离子作用更为明显。 相似文献
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用氮离子、碳离子不同能量、剂量组合注入４个甜菊品种的干种子中，播种后调查田间植株的生物学性状，结果表明：１）同一品种不同处理茎叶鲜重与对照比均有提高的趋势，其中以７５ｋｅＶ×１０１５／ｃｍ２氮离子注入处理为最佳，地上部鲜重及干重分别比对照高６２．３％、５４％，而６０ｋｅＶ×１０１４／ｃｍ２氮离子注入处理与对照比可促进总叶数及单株叶面积的增加；２）氮、碳离子作用比较结果表明，７５ｋｅＶ×１０１４／ｃｍ２与７５ｋｅＶ×５×１０１４／ｃｍ２处理碳离子对所有性状的作用均优于氮离子（７５ｋｅＶ×５×１０１４／ｃｍ２处理的株高性状除外），而７５ｋｅＶ×１０１５／ｃｍ２处理则氮离子作用均优于碳离子（唯有节数两离子作用相当）；３）４个参试品种比较结果表明，纯品种各性状表现均一致（济宁、丰２），而两个杂交种某些性状略有差异。 相似文献
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《中国糖料》2019,(4)
围绕甜叶菊种质评价及甜菊糖苷分子育种所需,针对甜叶菊叶片各甜菊糖苷组分含量分析用样品的准备,在前期的研究中本实验室建立了一种利用酸性氧化铝去除提取液中酚类等非甜菊糖苷杂质的前处理方法。为验证该样品前处理方法对非甜菊糖苷杂质及甜菊糖苷检出效果的影响,促进酸性氧化铝前处理方法在甜叶菊种质评估及甜菊糖苷提取加工业中的应用,本研究在对甜叶菊叶片样品提取液中甜菊糖苷UPLC分离体系建立的基础上,利用UPLC-MS就酸性氧化铝处理对甜叶菊叶片浸提液样品中甜菊糖苷和非甜菊糖苷类化合物检出情况进行了分析验证。结果表明,酸性氧化铝处理后,甜叶菊叶片浸提液中绝大部分非甜菊糖苷类杂质检出率下降70%以上,而甜菊糖苷检出率基本不受影响。揭示酸性氧化铝前处理可有效去除甜叶菊叶片提取液中非甜菊糖苷杂质对甜菊糖苷检测的影响,在甜叶菊种质评估及甜菊糖苷提取加工等过程检测样品的准备中可将酸性氧化铝作为有效的前处理物。 相似文献
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DA—6对甜菊叶片糖苷含量和组分影响研究 总被引：8，自引：1，他引：7
培育丰产、优质、高糖苷含量的甜菊新品种是关系甜菊资源利用的主要课题。本研究以３种不同浓度（２０×１０－６、３０×１０－６和４０×１０－６）ＤＡ－６处理甜菊叶片,不同时间（处理后７ｄ,１４ｄ和２１ｄ）取材,应用重量法测定总糖苷含量,ＨＰＬＣ分离检测两种主要糖苷组分（ＳＳ和ＲＡ）,显示ＤＡ—６对叶片总糖苷含量及主要组分的影响均呈一定的浓度效应和时间效应,并由此而筛选出有利于生产合适的ＤＡ—６处理方法。 相似文献
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离子注入甜菊种子生物效应的研究：Ⅱ.甜菊糖甙成分及其产量分析 总被引：3，自引：0，他引：3
用氮离子、碳离子不同能量、剂量组合注入４个甜菊品种的干种子,经田间栽培、收获时测定产量及糖甙含量变化,结果如下：１）同一品种（济宁种）不同处理,糖甙产量差异均达显著平准。其中以７５ｋｅＶ、５×１０１４／ｃｍ２氮离子注入组合最佳,公顷产干叶、总甙、Ｓｔ甙、ＲＡ甙产量分别比对照提高１５％、５％、３％及１４％。２）能量、剂量及离子作用比较,能量作用以６０ｋｅＶ可促进干叶产量提高;７５ｋｅＶ可促进糖甙产量的提高。剂量作用没有一定规律。初步看出５×１０１４对干叶产量及糖甙产量有促进作用。离子作用看出,氮离子可促进干叶产量提高,碳离子可促进糖甙产量的提高。３）４个参试品种比,以纯品种表现趋势较一致,杂交种变异大,如日原×丰２品种７５ｋｅＶ×１０１４碳离子注入组,干叶产量、总甙、Ｓｔ甙、ＲＡ甙及ＲＣ甙产量分别比对照高２３％、４２％、３９％、２８％及４３％,是个很有希望的育种材料。 相似文献
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混合体系多组分定量是现代分析科学研究难点,探讨建立利用液质联用(LC-MS)对作物器官所含各化合物进行定量的化学计量方法是化学形态学研究的基础,有利于作物品质育种亲本选配及栽培管理措施评价.本研究以甜叶菊叶片所含12种主要甜菊糖苷类化合物为例,通过考察归属各甜菊糖苷化合物的质荷比≥M/2的初级和次级裂解离子、分子离子、... 相似文献
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洪法水 《中国油料作物学报》1993,(4)
油菜种子秦油2号分别用不同浓度的CaCl_2及不同时间浸种处理。结果表明,在一定浓度和时间范围内,Ca~(2 )能够促进脂肪酶、过氧化物酶的活力、加强呼吸,提高发芽率。用0.3％CaCl_2溶液浸种24小时效果最佳。浸种时间过长和浓度过高,对油菜种子萌发及代谢有抑制作用。 相似文献
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启动氮加追肥对氮在大豆体内积累分配规律及产量的影响 总被引：2，自引：0，他引：2
采用框栽试验方法,在施用等量氮(纯N 50 kg.hm-2)条件下设置基肥一次性施用(对照);R3或R4期一次性追肥;启动氮15 kg加R3或R4期追氮35 kg共5个处理,研究启动氮加追肥对氮在大豆体内积累分配规律及产量的影响。结果表明,在R5期,与对照相比,启动氮15 kg加R4期追氮35 kg处理叶片N积累量减少11.5%,荚果N积累量增加36.6%(P<0.05);而根系N积累量增加21.7%(P<0.05),根瘤N积累量增加64.2%(P<0.05),表明启动氮加追肥处理N运转通畅,可以满足籽粒充实期大豆对氮素的需求。在R4～R8期,启动氮加R3或R4期追氮处理的氮积累量分别比基肥一次性施用处理增加82.6%和105%(P<0.01)。启动氮加R3或R4期追氮35 kg处理单株粒数分别较对照增加14.8%和15.8%(P<0.01),产量分别增加17.6%和19.9%(P<0.01)。大豆植株R4～R8期氮积累量与产量呈极显著正相关(r=0.795**),提高结荚鼓粒期后大豆氮素积累量是增加大豆产量的关键。 相似文献
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《Plant Production Science》2013,16(1):109-115
AbstractWe examined the effects of seeding rate, 50 or 150 seeds m-2, nitrogen (N) application rate at active tillering and jointing, 4 and 2 g N m-2, respectively, or none, and N application rate at anthesis, 0, 2, 4, or 6 g N m-2, on grain yield and protein content of a bread wheat cultivar, ‘Minaminokaori’, during the 2004–2005 crop season in southwestern Japan. Grain yield was similar at a seeding rate of 50 and 150 seeds m-2. It was higher when 4 and 2 g N m-2 were applied at active tillering and jointing, respectively (4–2N), than when no N was applied at these stages (0–0N). However, it was not influenced by N application rate at anthesis. Grain protein content was similar at 50 and 150 seeds m-2. It was higher in 4–2N than in 0–0N. It was the highest when 6 g N m-2 was applied at anthesis, followed by 4, 2, and 0 g N m-2. The SPAD value at anthesis was higher at 50 than 150 seeds m-2, but leaf area index (LAI) at anthesis was similar at 50 and 150 seeds m-2 and protein content of grain was nearly the same at 50 and 150 seeds m-2 irrespective of N application rate at anthesis. LAI and the SPAD value were higher in 4–2N than in 0–0N and the protein content of grain was also higher in 4–2N than in 0–0N irrespective of N application rate at anthesis. Therefore, both LAI and the SPAD value may be important traits related to the N application rate at anthesis suitable for yielding wheat grain with a high protein content. 相似文献
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Endosperm-Specific Expression of Serotonin <Emphasis Type="Italic">N</Emphasis>-Hydroxycinnamoyltransferase in Rice 总被引：1，自引：0，他引：1
Lee K Kang K Park M Woo YM Back K 《Plant foods for human nutrition (Dordrecht, Netherlands)》2008,63(2):53-57
Serotonin N-hydroxycinnamoyltransferase (SHT) is a key enzyme in the synthesis of feruloylserotonin (FS) and 4-coumaroylserotonin (CS). These serotonin derivatives show strong antioxidant activity, making them valuable for both nutritional and pharmacological use in humans. Ectopic expression of SHT under the control of the endosperm specific-glutelin and prolamin promoters from rice was produced via Agrobacterium-mediated transformation. SHT expression was confirmed by Southern blot analysis, followed by Northern blotting and SHT enzyme activity analyses using total RNA and protein, respectively, extracted from transgenic seeds. The glutelin A3 (GluA3) promoter produced low SHT mRNA expression in rice seeds, whereas the prolamin promoter expressed high levels of SHT mRNA. In spite of the ectopic expression of SHT in rice seeds, both transgenic genotypes accumulated levels of serotonin derivatives similar to those found in wild-type rice. Furthermore, our data suggest that serotonin, rather than phenylpropanid-CoAs, is the rate-limiting substrate in the biosynthesis of serotonin derivatives in SHT-overexpressing transgenic rice seeds. 相似文献
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Luis Raul Tovar E. Brito T. Takahashi T. Miyazawa J. Soriano K. Fujimoto 《Plant foods for human nutrition (Dordrecht, Netherlands)》1989,39(4):299-309
Amaranth was a major crop among the Aztecs. In Mexico the seed is popped and eaten with brown sugar. The crude protein content of the seed is 14±2% but its contents of lysine and tryptophan are 6.2 and 1.6 g/16 g N respectively. We developed a popping method based on a fluid bed system (FBS) whereas the traditional method (TM) is just to pop the seeds manually in a hot plate. Assays carried out were evaluation of racemization of the amaranth protein due to heat treatment, amino acid composition of the raw and heat treated seeds and a biological experiment testing whether leucine was the most limiting amino acid of amaranth protein. Male rats were fed both popped amaranths and roasted amaranth. Parboiled amaranth and casein were controls. The results were: (a) Lys, Arg and Cys were damaged in the heat treated seeds; (b) Asp, Met, Glu, Ala and Phe were racemized in that decreasing order in the seeds popped and roasted by the TM; (c) the estimated net protein retention (NPR) and estimated net protein utilization (NPU) of popped amaranths by either method were not different, but were lower than for the parboiled amaranth. The parboiled amaranth was not different from casein; (d) Leu was not the most limiting amino acid in any of the amaranth seeds tested. After Lys, sulfur amino acids appear to be the next most limiting in severely heat treated amaranth. The FBS seems to be a promising method for popping amaranth at industrial level. 相似文献
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《Plant Production Science》2013,16(3):187-193
SummaryTo confirm the excellence in productivity of the stored seeds of wheat and two-rowed barley, we examined the growth characters and productivity of the plants developed from the seeds stored at 5°C and 40% relative humidity for various periods but showed a 80% or higher germination percentage, in comparison with those of newly harvested seeds (new seeds). The cultivars of stored seeds were identified by the random amplified polymorphic DNA (RAPD) method. There were no differences in the morphological characters of the seedlings 3 months after emergence between the new seeds and the old seeds (stored for 2 years and 5 months) or the very old seeds (stored for 10 years and 5 months or 16 years and 5 months). On the other hand, there was a positive correlation between the top dry weight at 3 months after emergence and 1,000-grain weight of the seed. In the plants from the old seeds, no significant differences from those from new seeds were observed either in the mean heading date, maturing date, culm lengths, ear length, yield components, grain yield or inspection-grade. On the other hand, in the plants from the very old seeds, the mean heading date, maturing date, culm lengths, yield components and grain yield were significantly different from those in the new seeds in some cultivars. The results of RAPD analysis showed that the band pattern of the very old seeds was the same as that of the new seed in each cultivar. We concluded that the seeds of wheat and two-rowed barley stored for 2 years and 5 months with a germination percentage of higher than 80% are practically useful, showing normal growth characters and productivity. However, it should be noted that some cultivars had inferior grain yield after the seed storage for over 10 years. 相似文献
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为快速、自动、精准地测定油菜籽粒千粒重,建立一种基于图像处理技术,对不同品种的甘蓝型油菜籽粒进行分样、称取质量、获取图像并经图像处理得到表征籽粒面积的像素数,建立籽粒面积与质量之间的相关性模型.利用选择性极限腐蚀算法获得每个籽粒的核,并标记在籽粒的梯度图像上,再利用分水岭算法对标记的梯度图像进行分割.提取一次分割后仍然... 相似文献