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1.
调查了重庆市山羊隐孢子虫感染情况,应用饱和蔗糖溶液漂浮法对重庆市武陵山区和山峡库区6个种羊场共301份粪样进行了检测(其中每个羊场抽检率大于10％).结果检出隐孢子虫阳性样品16份,平均感染率为5.32％,场感染率为50％,均在山峡库区,种羊场阳性率最高为21.1％;不同年龄阶段感染情况为:以2～3月龄羔羊、产后1月和怀孕2月左右母羊的感染率较高,分别为9.68％、10.5％和6.67％.所检出的隐孢子虫卵囊经形态学观察,初步鉴定为微小隐孢子虫(C.Parvum). 相似文献
2.
为了解安徽省山羊隐孢子虫感染情况,为制定人畜共患隐孢子虫病的防控措施提供理论依据,在安徽省境内选泗县、涡阳县和霍邱县作为调查点,分别从各调查点山羊养殖场随机采集50、50、30份新鲜山羊粪样,用改良抗酸染色方法进行检测。共检获2种隐孢子虫,即微小隐孢子虫(Cryptosporidium parvum)和安氏隐孢子虫(C.andersoni);隐孢子虫阳性粪样均源自泗县,其感染率为36.00%(18/50),在涡阳县、霍邱县山羊粪样中未检出隐孢子虫。结论表明:羊感染隐孢子虫与饲养管理、自身免疫力等相关。 相似文献
3.
为了解元江县部分山羊肠道寄生虫感染状况,采用饱和食盐水漂浮法结合麦克马斯特氏法,对该县2个采样点6个不同养殖场中采集的168份山羊粪样进行了寄生虫检测及阳性粪样虫卵计数,并采用巢氏PCR方法对粪样中毕氏肠微孢子虫、芽囊原虫和隐孢子虫进行检测.结果显示,该地区山羊肠道寄生虫总感染率为44.64％,以线虫和球虫感染为主,球... 相似文献
4.
为了解河南省槐山羊肠道寄生虫的感染情况,应用寄生虫常规检测方法对周口市某规模化养羊场150份粪样进行寄生虫感染情况调查。结果共检出5类虫卵和卵囊,其中原虫2类(球虫、隐孢子虫)、线虫2类(类圆线虫、鞭虫)、绦虫1类。调查150份粪便样品全部为寄生虫阳性,寄生虫总感染率为100%,其中隐孢子虫感染率为5.33%,球虫的感染率为100%,鞭虫的感染率为10.67%,类圆线虫的感染率为57.33%,绦虫的感染率为44.67%。调查结果说明槐山羊肠道寄生虫混合感染现象较严重,寄生虫感染率呈随着年龄增长而降低的趋势。 相似文献
5.
目的了解合肥市犬隐孢子虫的感染情况。方法在合肥市随机采集不同年龄、性别与饲养条件的犬粪样69份,采用金胺-酚改良抗酸染色法检测粪样隐孢子虫感染情况,并根据卵囊大小和形态,进行虫种鉴定。结果犬隐孢子虫感染率为28.99%。对68个隐孢子虫卵囊进行观察和测量,椭圆形卵囊大小平均为6.20μm×4.34μm,卵囊形状指数平均为1.44,初步鉴定为鼠隐孢子虫（Cryptosporidium muris）;近圆形卵囊,大小平均为4.69μm×4.58μm,卵囊形状指数平均为1.125,初步鉴定为微小隐孢子虫（Cryptosporidium parvum）。结论犬隐孢子虫的感染率存在年龄差异,年龄越小,感染率越高;饲养管理条件差的犬隐孢子虫感染率高;而隐孢子虫感染率与性别没有关系。 相似文献
6.
为了解山东省滕州市羊隐孢子虫感染情况和种类,从该市2个绵羊场、2个山羊场采集222份羊粪便样品,提取所有样品的基因组DNA,采用巢式PCR扩增隐孢子虫actin基因,对阳性样品进行序列测定和分析。结果显示,滕州市羊隐孢子虫总的感染率为6.76%,其中绵羊7.84%,山羊5.83%;不同月龄的羊感染情况不同,6~12月龄绵羊感染率最高(20.69%),而12月龄以上的山羊感染率最高(10.00%);共发现3种隐孢子虫感染,其中微小隐孢子虫(Cryptosporidium parvum,C.parvum)检出率最高为53.33%,其次是肖氏隐孢子虫(C.xiaoi)为40.00%,仅发现1例(6.67%)泛在隐孢子虫(C.ubiquitum)感染;序列比对结果显示,本试验所获得基因序列与Gen Bank中的C.parvum、C.xiaoi、C.ubiquitum actin基因序列同源性达到100%;系统进化分析显示,滕州市的羊样品中鉴定的actin序列,与Gen Bank中的相应虫种序列在同一分支上。研究结果为深入了解滕州市羊隐孢子虫流行情况及制定有效的防控措施提供了依据。 相似文献
7.
为了解河南省槐山羊肠道寄生虫的感染情况,应用寄生虫常规检测方法对周口市某规模化养羊场150份粪样进行寄生虫感染情况调查。结果共检出5类虫卵和卵囊,其中原虫2类（球虫、隐孢子虫）、线虫2类（类圆线虫、鞭虫）、绦虫1类。调查150份粪便样品全部为寄生虫阳性,寄生虫总感染率为100％,其中隐孢子虫感染率为5．33％,球虫的感染率为100％,鞭虫的感染率为10．67％,类圆线虫的感染率为57．33％,绦虫的感染率为44．67％。调查结果说明槐山羊肠道寄生虫混合感染现象较严重,寄生虫感染率呈随着年龄增长而降低的趋势。 相似文献
8.
为了解宜宾市羊场羊群寄生虫感染情况,对2个养羊场的山羊携带寄生虫情况进行调查。共采集山羊粪便101份,采用饱和蔗糖漂浮法检测。结果显示,山羊寄生虫感染率为95.05%,且发现6种虫卵或卵囊:球虫、线虫、蛔虫、隐孢子虫、贾第虫、鞭虫,感染率分别为95.05%、61.39%、49.51%、9.90%、9.90%、11.88%。表明宜宾地区山羊寄生虫感染率较高。 相似文献
9.
以隐孢子虫(Cryptosporidium spp.)18S rRNA为靶基因,通过巢氏PCR检测发现在采集的101份新鲜粪便样品中18份样本为阳性。不同地区羊场的隐孢子虫感染率分别为：李集镇36%(18/50),新安镇0%(0/30)、堆沟港镇0%(0/21)未检出隐孢子虫。但通过对4羊场的分析发现,有2个羊场(50%)为隐孢子虫感染阳性,且不同的羊场感染率差异显著,因此单纯的以地区来评价隐孢子虫的感染率,是值得商榷的。山羊隐孢子虫的感染率为33.3%,湖羊隐孢子虫的感染率为2%。2～6月龄的育肥羊隐孢子虫的感染率为36%,6～10月龄的育成羊(0%)。对检测为阳性的样品进行了隐孢子虫18S rRNA基因片段序列分析,发现18个样品全部为肖氏隐孢子虫(Cryptosporidium xiaoi),不存在泛在隐孢子虫(Cryptosporidium ubiquitum)。在检测隐孢子虫感染阳性的1个山羊场和1个羊湖羊场均存在肖氏隐孢子虫感染,不存在泛在隐孢子虫,更未发现肖氏隐孢子虫和泛在隐孢子虫的混合感染。目前的数据提示肖氏隐孢子虫对2～6月龄山羊(33.3%)和湖羊(2%)具有更高的... 相似文献
10.
为了解河南省奶牛安氏隐孢子虫病流行情况,采用饱和蔗糖溶液漂浮法和改良抗酸染色法对河南省12个规模化奶牛场和7个奶牛养殖小区共计2 268份粪便样本进行检查,发现109份为阳性粪便样本,感染率为4.8%。卵囊呈长椭圆形,平均大小为7.8μm×6.4μm,卵囊指数为1.22;12个养殖场中10个为阳性场,7个养殖小区中4个为阳性小区,不同地区奶牛安氏隐孢子虫感染率统计学差异显著(P0.05);不同年龄段奶牛安氏隐孢子虫感染率统计学差异极显著(P0.01);不同养殖方式奶牛隐孢子虫感染率统计学差异不显著(P0.05)。结果表明:奶牛隐孢子虫感染流行范围较广,存在一定人兽共患风险。 相似文献
11.
In late December 1983, 2 800 veterinarians across Canada were sent a questionnaire in order to assess the status of heartworm disease in Canada in 1983 and 26% of them responded. Veterinarians reported that 59 504 dogs were blood-tested to check for microfilariae and 771 dogs (1.30% of those tested) were found with Dirofilaria immitis. Heartworm disease was diagnosed in all provinces except New Brunswick and Newfoundland but most (733) of the cases were in Ontario. Heartworm disease was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 31% of the cases were observed with clinical signs of heartworm disease and 64% had a history of not having left Canada. Southwestern Ontario continues to be the focus of the infection and most of the dogs there had not left the province previously. 相似文献
13.
In late December 1985, 1485 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1985 and 44% of them responded. Veterinarians reported that 137,300 dogs were blood-tested to check for microfilariae and 1210 dogs were found with heartworm. Another 36 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number diagnosed in 1985 as 1247 (0.91%). Heartworm was reported from all provinces except Prince Edward Island, Newfoundland and Saskatchewan but most (1126) of the cases were in Ontario. Southwestern Ontario continued to be the primary focus of the infection in Canada. From Quebec, 91 cases were reported mostly from and around Montreal. From Manitoba, 19 cases were reported from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 28% of the cases were observed with clinical signs of heartworm disease and 78% had a history of not having left Canada. 相似文献
14.
In late November 1988, 1581 small and mixed animal clinics and institutional veterinarians across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1988, and 46% of them responded. Veterinarians reported that 181,577 dogs were blood-tested for heartworm disease and 367 dogs were found with D. immitis microfilariae. Another 60 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1988 as 441 (0.24%). 相似文献
15.
In late December 1984, 1853 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1984 and 35% of them responded. Veterinarians reported that 97,794 dogs were blood-tested to check for microfilariae and 1417 dogs (1.45% of those tested) were found with heartworm. Another 34 dogs were amicrofilaremic, but were diagnosed as having heartworm disease, to give the total number diagnosed in 1984 as 1451 (1.48%). Heartworm was reported from all provinces except Prince Edward Island and Newfoundland but most (1310) of the cases were in Ontario. In Quebec, 126 cases were reported mostly from west of Montreal. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 27% of the cases were observed with clinical signs of heartworm disease and 72% had a history of not having left Canada. Southwestern Ontario continued to be the primary focus of the infection. 相似文献
17.
In late November 1991, 1883 clinics in Canada were sent a questionnaire to assess the status of Dirofilaria immitis in dogs in 1991 and there was a 60.0% response. There were 344,031 dogs tested for heart-worm (HW), 627 were found infected and the prevalence of HW infection was 0.18%. There were 417 dogs with HW in Ontario, 116 in Manitoba, 38 in Quebec, 53 in British Columbia, three in Alberta, and one in Nova Scotia. In British Columbia, all of the infected dogs but one were from the Okanagan valley which, as from 1991, is a new focus of infection in Canada. Most dogs with HW had not been on preventive medication in 1990, and the prevalence among dogs tested and unprotected was 0.59%. That prevalence was considerably higher in endemic areas. Companion dogs, over three years of age and maintained primarily outdoors in rural areas, were most frequently infected. One cat was diagnosed with D. immitis and 33 dogs had Dipetalonema reconditium. 相似文献
18.
In late November 1989, 1732 clinics and institutional veterinarians were sent a questionnaire to assess the status of Dirofilaria immitis, and 51.7% responded. Of 247,716 dogs tested, 394 had D. immitis microfilariae and 51 were amicrofilaremic for a total of 445 cases and heartworm prevalence of 0.17%. Most (408) of these dogs had no preventive medication and the prevalence among dogs tested and unprotected was 1.01%. That prevalence was considerably higher in endemic areas. Thirty-seven dogs with heartworm had preventive medication. Heartworm was most frequent in companion dogs over three years of age maintained outdoors in rural areas. About 75% of the cases had never left Canada, 26% had clinical signs and 125 were not treated. Heartworm was reported from British Columbia, Manitoba, Ontario, Quebec, Nova Scotia and Newfoundland, but 383 cases were in Ontario. South-western Ontario was the primary focus of infection. There were 33 cases in Quebec and 24 in Manitoba, mainly found in and around Metropolitan Montreal and Winnipeg respectively. 相似文献
19.
In late November 1987, 1246 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1987, and 50% of them responded. Veterinarians reported that 165,428 dogs were blood tested for heartworm disease and 511 dogs were found with D. immitis microfilariae. Another 78 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1987 as 589 (0.35%). 相似文献
20.
In late December 1986, 1224 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1986; 46% of them responded. Veterinarians reported that 150,989 dogs were blood-tested for microfilariae and 869 dogs were found with heartworm. Another 65 dogs were amicrofilaremic but diagnosed with heartworm disease and one was found with heartworm at necropsy to give the total number diagnosed in 1986 as 935 (0.62%). Heartworm was reported from Manitoba, New Brunswick, Ontario and Quebec, but most (810) of the cases were from Ontario. South-western Ontario continued to be the primary focus of the infection in Canada. There were 103 cases reported from Quebec, mostly from and around Montreal, and 21 cases from Manitoba, from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 33% of the cases were observed with clinical signs of heartworm disease and 81% had a history of not having left Canada. 相似文献
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