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1.
为了解开封地区奶牛肠道寄生虫感染情况,采用离心沉淀法、卢戈氏碘液染色法和饱和蔗糖溶液漂浮法对4个奶牛养殖场和5个奶牛养殖小区共622份新鲜粪便样本进行检查。共检出7种肠道寄生虫,总感染率为41.6%,以球虫为主要感染虫种,感染率33.4%,贾第虫、隐孢子虫、环孢子虫、食道口线虫、鞭虫和莫尼茨绦虫感染率分别为3.4%、8.4%、0.3%、0.8%、0.6%和0.3%。结果分析显示,不同调查点奶牛肠道寄生虫感染率统计学差异极显著(P<0.01);不同年龄段奶牛肠道寄生虫感染率统计学差异极显著(P<0.01);不同饲养条件下奶牛肠道寄生虫感染率统计学差异极显著(P<0.01)。 相似文献
2.
为了解动物园动物肠道寄生虫感染情况,本试验采集了来自贵阳和北京动物园33种动物,共150份粪便样品。采用饱和盐水漂浮法和离心沉淀法富集虫卵和卵囊进行显微镜检查;基于隐孢子虫、芽囊原虫、毕氏肠微孢子虫和十二指肠贾第虫的特异性基因位点对人兽共患原虫进行PCR检测和序列比对分析。结果显示,动物园动物寄生虫总感染率为64.0%。显微镜检查出的寄生虫包括毛首线虫(20.0%)、细颈线虫(2.7%)、艾美耳球虫(14.7%)和其他线虫(25.3%)。PCR检测4种肠道原虫的感染率分别为：隐孢子虫1.3%、毕氏肠微孢子虫8.7%、十二指肠贾第虫12.0%和芽囊原虫32.0%。序列比对结果显示,存在2种隐孢子虫虫种(安氏隐孢子虫和泛在隐孢子虫);7种毕氏肠微孢子虫基因型(SC02、BEB6、Type IV、PigEBITS 7、Peru8、PtEb IX和D);2种十二指肠贾第虫基因型(B和E);8种芽囊原虫基因亚型(ST1、ST2、ST3、ST5、ST8、ST10、ST13和ST14)。结果表明,贵州和北京动物园动物肠道寄生虫感染非常普遍,并且存在多种人兽共患虫种和基因型。做好动物园寄生虫病的防控不... 相似文献
3.
为了解安阳地区羊肠道寄生虫感染情况,采用显微镜检测技术对滑县、内黄县、汤阴县、林州市和安阳县共计880份羊粪便样品进行了寄生虫感染情况调查。结果检测到球虫、贾第虫、隐孢子虫、圆线虫、毛尾线虫、绦虫和吸虫7种肠道寄生虫,共发现589份寄生虫阳性粪便样品,寄生虫总感染率为66.93%(589/880)。其中,球虫感染率为55.00%,贾第虫感染率为1.82%,隐孢子虫感染率为0.34%,圆线虫感染率为18.86%,毛尾线虫感染率为2.16%,绦虫感染率为2.39%,吸虫感染率为2.73%,以球虫和圆线虫为本地区优势虫种。 相似文献
4.
[目的]了解山东省菏泽地区奶牛肠道寄生虫感染现状,为奶牛寄生虫防控提供参考。[方法]在菏泽地区选择8家养殖场,按犊牛（<6月龄）、育成牛（7～16月龄）、成年牛（>16月龄）分类,采用直肠法收集奶牛新鲜粪便1 438份,利用离心沉淀法、饱和蔗糖漂浮法及卢戈氏碘液染色法,进行显微镜检测。[结果]寄生虫总感染率33.45%;球虫、阿米巴原虫、隐孢子虫、圆线虫、贾第虫、绦虫、鞭虫感染率分别为27.33%、 7.65%、 3.27%、3.48%、0.56%、0.49%、1.32%;优势感染虫种为球虫;混合感染率为10.85%,混合虫体种类2～4个。各场点和年龄阶段奶牛均感染寄生虫。[结论]菏泽地区奶牛肠道寄生虫感染较普遍,养殖场需采取防控措施。 相似文献
5.
为明确内蒙古某规模化奶牛养殖场腹泻犊牛寄生虫性病原的感染情况及其分子学特性,采集该养殖场18头发生腹泻犊牛的新鲜粪便样品。通过形态学方法并结合分子生物学方法对样品中隐孢子虫(Cryptosporidium)和蓝氏贾第虫(Giardia lamblia)进行分离鉴定和感染情况统计分析。形态学检查结果表明,18份粪便样品中隐孢子虫感染率为5.56%(1/18),蓝氏贾第虫感染率为72.22%(13/18)。进一步对18份粪便样品提取DNA,并基于SSU rRNA和BG基因位点分别对隐孢子虫和蓝氏贾第虫进行PCR扩增、序列比对和遗传进化分析。电泳检测结果显示,显微镜检测阳性粪便样品均在830 bp和511 bp处出现目的扩增条带。同时,序列比对及遗传进化分析也表明,所分离到的隐孢子虫与来自于不同国家和地区的牛隐孢子虫(C.bovis)在SSU rRNA基因位点上具有100%序列同源性,因此被归于同一个进化分支,而所分离的蓝氏贾第虫在BG基因位点上与集聚体E参考分离株的序列同源性高达99.78%以上,提示其属于集聚体E。通过形态学和分子学方法明确了牛隐孢子虫和蓝氏贾第虫是该奶牛场主要致犊牛腹泻... 相似文献
6.
贾第虫、隐孢子虫和微孢子虫市最重要的奶牛肠道原虫,可以感染多种动物,如宠物和其他家畜,同时也可以感染人,是人畜共患的传染病。本文重点讲述贾第虫流行病学调查研究,为我市的养牛业对原虫的防控提供理论参考。 相似文献
7.
为确定导致犊牛腹泻的寄生原虫种类及分子学特性,利用基于隐孢子虫核糖体小亚基RNA、蓝氏贾第虫β-贾第素和毕氏肠微孢子虫内转录间隔区的分子生物学技术对腹泻犊牛的粪便样品进行鉴定。结果表明,引起犊牛腹泻的寄生原虫包括微小隐孢子虫、安氏隐孢子虫、瑞氏隐孢子虫、E型蓝氏贾第虫和多种基因型的毕氏肠微孢子虫,并且存在多种原虫混合感染。说明原虫是引起犊牛腹泻的重要病原,畜牧业临床中应加强原虫病的防控。 相似文献
8.
为了解宠物兔肠道寄生虫感染情况,采用卢戈氏碘液染色法和饱和蔗糖溶液漂浮法对1个宠物市场和2个宠物兔场的305份兔新鲜粪便样品进行检查。结果检出肠道寄生虫5种,总感染率为71.2%,其中球虫感染率最高,为47.2%,隐孢子虫、贾第虫、圆线虫和栓尾线虫感染率分别为2.0%、3.9%、29.5%和1.0%;不同采样点和不同年龄段宠物兔肠道寄生虫的感染率统计学差异均极显著(P<0.01),寄生虫感染随年龄增长呈递减趋势。数据表明宠物兔肠道寄生虫感染率较高,且存在人兽共患机会性原虫,应加强其寄生虫病的防治。 相似文献
9.
为了解河北及天津近渤海地区奶牛肠道寄生虫感染情况,本试验采用离心沉淀法、饱和蔗糖漂浮法及卢戈氏碘液染色法对河北和天津地区12个奶牛场1 040份奶牛粪便样品进行显微镜检测。结果显示,寄生虫总感染率为61.63%,其中河北及天津地区寄生虫感染率分别为59.78%和66.12%。共检测出8种寄生虫,即球虫、贾第虫、隐孢子虫、阿米巴、圆线虫、鞭虫、绦虫及吸虫,感染率分别为40.96%、1.63%、8.46%、33.27%、2.02%、3.56%、0.96%和0.96%,其中球虫与阿米巴为优势虫种。本试验结果表明,河北及天津地区奶牛肠道寄生虫感染比较普遍,养殖场需采取必要的防控措施。 相似文献
10.
为了解宠物犬肠道寄生虫,尤其是人兽共患肠道寄生虫感染情况,采用甲醛-乙酸乙酯沉淀法、卢戈氏碘液染色法和饱和蔗糖溶液漂浮法对郑州市宠物犬404份粪便样品进行了调查。结果共发现10种肠道寄生虫,寄生虫总感染率为37.12%,其中球虫和贾第虫为优势虫种,感染率分别为18.32%和12.62%;7种为人兽共患虫种,贾第虫、隐孢子虫、钩虫、弓首蛔虫、狮弓蛔虫、粪类圆线虫和犬复孔绦虫感染率分别为12.62%、3.71%、3.96%、6.93%、0.20%、1.73%和0.50%;2～3种寄生虫混合感染率为9.90%。结果分析表明,寄生虫感染与样品采集地点和犬年龄统计学差异极显著(P0.01),与性别无统计学差异(P0.05)。宠物犬肠道寄生虫感染较为普遍,人兽共患寄生虫流行率较高,应加强对其监控和防治。 相似文献
11.
In late December 1984, 1853 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1984 and 35% of them responded. Veterinarians reported that 97,794 dogs were blood-tested to check for microfilariae and 1417 dogs (1.45% of those tested) were found with heartworm. Another 34 dogs were amicrofilaremic, but were diagnosed as having heartworm disease, to give the total number diagnosed in 1984 as 1451 (1.48%). Heartworm was reported from all provinces except Prince Edward Island and Newfoundland but most (1310) of the cases were in Ontario. In Quebec, 126 cases were reported mostly from west of Montreal. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 27% of the cases were observed with clinical signs of heartworm disease and 72% had a history of not having left Canada. Southwestern Ontario continued to be the primary focus of the infection. 相似文献
13.
In late December 1983, 2 800 veterinarians across Canada were sent a questionnaire in order to assess the status of heartworm disease in Canada in 1983 and 26% of them responded. Veterinarians reported that 59 504 dogs were blood-tested to check for microfilariae and 771 dogs (1.30% of those tested) were found with Dirofilaria immitis. Heartworm disease was diagnosed in all provinces except New Brunswick and Newfoundland but most (733) of the cases were in Ontario. Heartworm disease was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 31% of the cases were observed with clinical signs of heartworm disease and 64% had a history of not having left Canada. Southwestern Ontario continues to be the focus of the infection and most of the dogs there had not left the province previously. 相似文献
15.
In late November 1991, 1883 clinics in Canada were sent a questionnaire to assess the status of Dirofilaria immitis in dogs in 1991 and there was a 60.0% response. There were 344,031 dogs tested for heart-worm (HW), 627 were found infected and the prevalence of HW infection was 0.18%. There were 417 dogs with HW in Ontario, 116 in Manitoba, 38 in Quebec, 53 in British Columbia, three in Alberta, and one in Nova Scotia. In British Columbia, all of the infected dogs but one were from the Okanagan valley which, as from 1991, is a new focus of infection in Canada. Most dogs with HW had not been on preventive medication in 1990, and the prevalence among dogs tested and unprotected was 0.59%. That prevalence was considerably higher in endemic areas. Companion dogs, over three years of age and maintained primarily outdoors in rural areas, were most frequently infected. One cat was diagnosed with D. immitis and 33 dogs had Dipetalonema reconditium. 相似文献
16.
In late December 1985, 1485 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1985 and 44% of them responded. Veterinarians reported that 137,300 dogs were blood-tested to check for microfilariae and 1210 dogs were found with heartworm. Another 36 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number diagnosed in 1985 as 1247 (0.91%). Heartworm was reported from all provinces except Prince Edward Island, Newfoundland and Saskatchewan but most (1126) of the cases were in Ontario. Southwestern Ontario continued to be the primary focus of the infection in Canada. From Quebec, 91 cases were reported mostly from and around Montreal. From Manitoba, 19 cases were reported from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 28% of the cases were observed with clinical signs of heartworm disease and 78% had a history of not having left Canada. 相似文献
17.
In late November 1988, 1581 small and mixed animal clinics and institutional veterinarians across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1988, and 46% of them responded. Veterinarians reported that 181,577 dogs were blood-tested for heartworm disease and 367 dogs were found with D. immitis microfilariae. Another 60 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1988 as 441 (0.24%). 相似文献
18.
In late November 1989, 1732 clinics and institutional veterinarians were sent a questionnaire to assess the status of Dirofilaria immitis, and 51.7% responded. Of 247,716 dogs tested, 394 had D. immitis microfilariae and 51 were amicrofilaremic for a total of 445 cases and heartworm prevalence of 0.17%. Most (408) of these dogs had no preventive medication and the prevalence among dogs tested and unprotected was 1.01%. That prevalence was considerably higher in endemic areas. Thirty-seven dogs with heartworm had preventive medication. Heartworm was most frequent in companion dogs over three years of age maintained outdoors in rural areas. About 75% of the cases had never left Canada, 26% had clinical signs and 125 were not treated. Heartworm was reported from British Columbia, Manitoba, Ontario, Quebec, Nova Scotia and Newfoundland, but 383 cases were in Ontario. South-western Ontario was the primary focus of infection. There were 33 cases in Quebec and 24 in Manitoba, mainly found in and around Metropolitan Montreal and Winnipeg respectively. 相似文献
19.
In late November 1987, 1246 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1987, and 50% of them responded. Veterinarians reported that 165,428 dogs were blood tested for heartworm disease and 511 dogs were found with D. immitis microfilariae. Another 78 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1987 as 589 (0.35%). 相似文献
20.
In late December 1986, 1224 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1986; 46% of them responded. Veterinarians reported that 150,989 dogs were blood-tested for microfilariae and 869 dogs were found with heartworm. Another 65 dogs were amicrofilaremic but diagnosed with heartworm disease and one was found with heartworm at necropsy to give the total number diagnosed in 1986 as 935 (0.62%). Heartworm was reported from Manitoba, New Brunswick, Ontario and Quebec, but most (810) of the cases were from Ontario. South-western Ontario continued to be the primary focus of the infection in Canada. There were 103 cases reported from Quebec, mostly from and around Montreal, and 21 cases from Manitoba, from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 33% of the cases were observed with clinical signs of heartworm disease and 81% had a history of not having left Canada. 相似文献
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