共查询到20条相似文献，搜索用时 15 毫秒
1.
Isolation, purification and identification of polysaccharides from cultured Cordyceps militaris 总被引：8，自引：0，他引：8
Four polysaccharides from the water extract of cultured Cordyceps militaris were isolated through ethanol precipitation, deproteination and gel-filtration chromatography. Their molecular weights were determined using gel-filtration chromatography. Among the four isolated polysaccharides, the structures of two of them (CPS-2 and CPS-3) were elucidated by sugar analysis, Smith degradation, IR and (13)C-NMR spectroscopy. 相似文献
2.
A water-soluble polysaccharide (CPS-2), isolated from the cultured Cordyceps sinensis, was obtained by hot-water extraction, anion-exchange and gel permeation chromatography. Its structural characteristics were investigated by PMP pre-column derivation, periodate oxidation, methylation analysis, FTIR and NMR spectroscopy. CPS-2 was found to be mostly of α-(1 → 4)-d-glucose and α-(1 → 3)-D-mannose, branched with α-(1 → 4,6)-d-glucose every twelve residues on average. CPS-2 had a molecular weight of 4.39 × 104 Da. The protective effect of CPS-2 on the model of chronic renal failure was established by fulgerizing kidney. The changes in blood urea nitrogen and serum creatinine revealed that CPS-2 could significantly relieve renal failure caused by fulgerizing kidney. 相似文献
3.
红豆杉枝叶经热水浸提、浓缩、醇沉、透析等工序制得一种红豆杉多糖,采用SephadexG-100凝胶渗透法测定分子质量,采用气相色谱法测定其单糖组成,并测定了不同纯度红豆杉多糖的红外光谱。所得红豆杉多糖分子质量约为59.2 ku,可视为由约365个糖残基构成的高分子化合物,其单糖残基鼠李糖、阿拉伯糖、甘露糖、葡萄糖、半乳糖数量比约为4∶6∶1∶1∶4。该红豆杉多糖分子结构中含有普通单糖、糖醛酸或氨基酸残基等,单糖主要以吡喃环形式存在、通过β-(1,3)苷键结合形成一种糖蛋白缀合物,Sevag法处理不能完全除去分子中的肽链。 相似文献
4.
灵芝是一种木材腐朽菌,具有良好的药用价值,在中国和其它一些国家享有盛誉。作者对赤(灵)芝(Ganoderma lucidum)子实体多糖的提取及其理化性质进行了研究。结果表明,粗多糖得率为1.38％,纯化后的糖经薄层色谱和气相色谱分析查明含有木糖、甘露糖、阿拉伯糖、半乳糖和葡萄糖,糖基比依次为1:1:1.7:5:20。用凝胶过滤得分于量约为23000和34000。经红外光谱测定该多糖在1050、1140、1420和3400cm~(-1)处均有明显的吸收峰。本文提出了一个从灵芝子实体分离和纯化多糖体的方法。 相似文献
5.
酶法提取黑木耳多糖的纯化与鉴定 总被引：1，自引：0，他引：1
利用优化改进的Sevag试剂法对酶法提取的黑木耳粗多糖进行蛋白质的脱除。采用SephadexG-200凝胶层析对黑木耳粗多糖进行分级纯化，得到两个组分AAP1、AAP2，合并收集液，透析、浓缩、冻干得到黑木耳纯多糖，为白色粉末。采用SephroseA-200凝胶层析、紫外光谱扫描对黑木耳纯多糖进行纯度鉴定。SephroseA-200凝胶层析结果表明，多糖的两种组分都为单一洗脱峰；紫外光谱扫描说明多糖中不合有蛋白质、多肽及核酸物质；说明黑木耳多糖的纯度已经达到物理化学的分析标准。红外光谱扫描的结果表明，黑木耳多糖中合有多糖类物质的特征吸收峰。纸层析法对黑木耳纯多糖进行组分分析的结果表明黑木耳多糖由葡萄糖、木糖、半乳糖、甘露糖、阿拉伯糖组成，是一种杂多糖。 相似文献
6.
《Fitoterapia》2014
A novel water-soluble polysaccharide, designated Bletilla striata polysaccharide b (BSPb), was isolated from a traditional Chinese medicinal herb, Bletilla striata. The crude polysaccharide was obtained by hot water extraction and purified by ion-exchange chromatography. The molecular weight was 260 kDa as determined by HPGPC (high performance gel permeation chromatography). Monosaccharide analysis showed that BSPb contained glucose and mannose with a molar ratio of 3:1. Methylation and spectroscopic analyses with 1D and 2D NMR spectroscopy showed that the backbone of BSPb is mainly composed of (1 → 2)-linked α-d-mannopyranose and (1 → 4)-linked β-d-glucopyranose residues. In this study, BSPb plays an important role in protection against the renal fibrosis effect, which is probably mediated by down-regulated TGF-β RI, TGF-β RII, and α-SMA in vitro. 相似文献
7.
Ganoderma lucidum is a traditional medicinal herb used to treat various diseases in China and Southeast Asia for thousands of years. An aqueous glycopeptide, LZ-B-1, was prepared by successive chromatography and exhibited an immunostimulating potential. To better understand the mechanism of bioactivity for this compound, the polysaccharide moiety of glycopeptide LZ-B-1 was studied by NMR spectroscopy. The results indicated that the polysaccharide moiety had a backbone of 1,6-disubstituted-α-galactopyranosyl, 1,2,6-trisubstituted-α-galactopyranosyl, 1,3-disubstituted-β-glucopyranosyl and 1,4,6-trisubstituted-β-glucopyranosyl residues. The branches were mainly composed of 1-substituted-β-glucopyranosyl and 1-substituted-α-fucopyranosyl residues. 相似文献
8.
碱性溶液提取荞麦水溶性多糖的研究 总被引：1，自引：0，他引：1
研究用碱溶液（0．3mol／LNaOH）提取养麦水溶性多糖,分别用料液比（1：30,1：40,1：50）和提取时间（1h,2h,3h）,提取温度（70℃,80℃,90℃）3因素3水平正交法提取多糖,并研究了这3个因素对多糖得率的影响,方差分析表明温度对多糖提取影响较显著（P〈0．1）,料液比和时间影响不显著。在本实验条件下,荞麦多糖的得率迭25．16％。同时用Sevag方法除蛋白,石油醚回流脱脂,淀粉酶,纤维素酶除去淀粉和纤维素,双氧水脱去色素对多糖进行了纯化。 相似文献
9.
Zhang Yu Gu Ming Wang Kaiping Chen Zhixiang Dai Liquan Liu Jingyu Zeng Fang 《Fitoterapia》2010,81(8):1163-1170
A water-soluble polysaccharide LT1 was isolated from the basidiocarps of Lentinus edodes by hot water extraction and ethanol precipitations, further purified by gel chromatography. The Mw of LT1 was estimated to be 642 kDa by using HPGPC. Chemical and spectroscopic studies illustrated that LT1 has a backbone chain composed of 1 → 4-linked and 1 → 3-linked glucopyranosyl residues and has branches of single glucosyl stubs at C-6 of β-(1 → 4)-linked glucopyranosyl. AFM and Congo-red test revealed that LT1 existed as triple helix chain in 0.10 M NaOH solution or distilled water. Our studies showed that LT1 presented significant antitumor bioactivities on Sarcoma180 solid tumor cell implanted in BALB/c mice, which implies that LT1 could be potentially applied as a natural antitumor drug. 相似文献
10.
多糖是醋栗果实中具有重要生理功能的活性成分。以醋栗果实为原料,以醋栗多糖得率为指标,采用响应面法优化醋栗多糖的制备工艺。结果表明,在4种影响因素中,提取温度与提取时间的交互作用对醋栗多糖得率的影响最为显著;提取时间44min,超声功率100W,提取温度57℃,水料比为29∶1,在该条件下醋栗多糖得率为15.79%。该结果为醋栗果实多糖提取工艺研究及功能性成分的开发提供一定的理论基础。 相似文献
11.
大叶金花草多糖的提取、分离纯化及结构分析 总被引：7，自引：3，他引：4
采用热水提取法从大叶金花草中提取大叶金花草多糖,用酶法和Seveg法去除蛋白质,H2 O2脱色,经DEAE-纤维素(Cl -)柱层析纯化后,纸层析和Sephadex G-100 凝胶柱层析证实为单一组分,并用凝胶色谱法、薄层层析、乙酰化GC-MS、紫外光谱和红外光谱等对其组成和结构进行了研究.大叶金花草多糖平均分子质量为8.6×104 u,由阿拉伯糖、葡萄糖和糖醛酸组成,在260和 280 nm处有多糖无核酸和蛋白质的吸收峰.大叶金花草多糖是含有β-吡喃葡萄糖的非蛋白类单一多糖. 相似文献
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14.
Codonopsis pilosula is a perennial species of flowering plants propagated in Northeast Asia. A water-soluble polysaccharide, CPPS3, was extracted from the root of Codonopsis pilosula by boiled water extraction and ethanol precipitation. The molecular weight was estimated to be 7.4 × 104 Da determined by using Gel permeation chromatography. Monosaccharide composition and the structure of the polysaccharide were determined by gas spectroscopy, Fourier transform IR (FT-IR) spectroscopy, NMR spectroscopy and mass spectroscopy and some chemical method analysis was made. The components were galactose, arabinose and rhamnose in the molar ratio of 1.13:1.12:1. The main chain of CPPS3 is illustrated to be (1→3)-linked-β-GalpNAc, (1→3)-linked-α-Rhap and (1→2,3)-β-Galp. 相似文献
15.
A water-soluble polysaccharide, GPB, was obtained from leaf of Ginkgo biloba L by hot water extraction followed by precipitation with ethanol and fractionation with gel chromatography. The results of HPLC with TSK-GEL column and gel filtration chromatography with Sepharose CL-6B analysis indicated GPB was uniform in polarity and its molecular weight (MW) was about 10 kDa. The structure of GPB was analysed using chemical methods, IR spectroscopy, and NMR spectroscopy. GPB has a high branched structure with polygalactose as core part of backbone. The repeating unit of polygalactose consists of 1,6-linked Galactose (Gal) and 1,3,6-linked Gal. 相似文献
16.
对牡丹开花前后根、枝条的各种营养成分含量进行了分析,结果表明:开花前、后总糖、多糖、二糖、粗蛋白、粗纤维、果糖、N、P、Ca、Mg、S的含量变化都达到显著水平,说明牡丹的开花过程是一个明显能量消耗的过程.从牡丹开花前后根、枝条的各种营养成分含量来看,根中多糖、葡萄糖、总糖、淀粉、粗脂肪的含量明显高于枝条,而枝条中二糖、粗纤维的含量明显高于根,充分证明了牡丹根是植株的主要养分贮存器官,为开花提供所需营养,当年形成的枝条为下一次开花提供较少的养分,更主要的是起运输通道的作用. 相似文献
17.
微波、超声波萃取技术提取松茸多糖的研究 总被引：4，自引：0，他引：4
松茸多糖因具有抗肿瘤、增强免疫力、降血脂、抗病毒等生物活性作用,而越来越受到人们的重视,黑龙江林副特产研究所对松茸多糖的提取进行了研究。1）采用微波萃取技术,对微波萃取功率、微波萃取时间、料液比3个因素进行正交试验和分析,确定微波萃取的最佳工艺参数为微波功率660W,萃取时间45min,料液比1：25;2）采用超声波技术对松茸中水溶性粗多糖提取工艺进行了优化,并用硫酸-蒽酮法测定了松茸多糖的含量,结果表明,在超声功率180W、超声时间13min,提取温度100℃的条件下进行提取,料液比1：20,多糖提取率最高;3）比较超声和微波萃取松茸多糖的提取率,可知微波萃取方法更加优于超声方法,有更加广阔的应用前景。 相似文献
18.
An ultrasonic technique was employed to extract polysaccharides from Ophiopogon japonicus. The ultrasonic extracted polysaccharides (POJ-U) were purified, and POJ-U1a (a homogeneous fraction) was obtained. The structural characteristics of POJ-U1a were investigated by infrared spectra, gas chromatography, high performance liquid chromatography, periodate oxidation, Smith degradation, methylation analysis, gas chromatography–mass spectrometry and nuclear magnetic resonance spectroscopy. The results indicated that the relative molecular weight of POJ-U1a was 4.02 × 103 Da. POJ-U1a was an α-configuration polysaccharide with a highly branched structure, and consisted of pyranoside and funanside. The backbone of POJ-U1a consisted of 1,6-α-d-glucopyranose and 1,3,6-α-d-glucofuranose in the molar ratio of 7:3, while the branched chains were mainly composed of 1,3-α-d-glucopyranose and 1-α-d-glucopyranose in the molar ratio of 1:3. The branched structure of POJ-U1a was proved intuitively by AFM. Significant antioxidant activity of POJ-U1a has been proved as shown by its DPPH radical scavenging, hydrogen radical scavenging and superoxide anion scavenging activities, which indicated that POJ-U1a showed strong antioxidant activity. 相似文献
19.
松茸在防癌、抗肿瘤、降低血糖、提高机体免疫力、防治心血管疾病等方面效果显著;绿茶中营养成分众多,也具有很好的保健功能。以绿茶和松茸为原料研究的松茸速溶茶,既具有松茸的营养功效,又保留了绿茶的清香高爽,饮用方便。结果表明：确定绿茶浸提的最佳参数为：料液比1∶13,温度为65℃,浸提时间为40min;松茸速溶茶的最佳配方为：松茸多糖浸提液与绿茶浸提液比例为1∶5,柠檬酸为0．04％,甜味剂为10％（白砂糖∶蜂蜜＝3∶2）,产品为浅棕色、颜色发亮,具有松茸和绿茶特有的香味,酸甜可口,感官评定得分为92．1。 相似文献
20.
真菌多糖的研究进展——真菌多糖的结构、提纯和应用 总被引：2，自引：0，他引：2
真菌多糖是从真菌的子实体、菌丝体和真菌发酵液中分离出来的一类具有广泛生物活性的大分子碳水化合物。现代医学与药理学研究表明,多糖具有提高免疫力、抗肿瘤、延缓衰老、降血糖、降血压、降血脂等生理功效。综述了真菌多糖的化学组成,构效关系,提取与纯化,以及实际应用等现状与展望。 相似文献