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以'户太8号'和'夏黑'葡萄为试材,前者采用11组赤霉酸(GA3)和氯吡脲(CPPU)进行处理,后者采用8组处理,均以清水为对照,研究了不同GA3和CPPU处理下葡萄的外观形状和内在品质,以期为生产优质、健康的葡萄提供参考依据.结果表明:GA3和CPPU处理的单果质量为6.00~12.00 g(对照为3.00 g左右)、果形指数1.05~1.25(对照小于1.05)、果皮硬度7.50~11.00 N(对照小于7.00 N);果面色泽均显著增强;'户太8号'葡萄的可溶性固形物、总酸含量以及固酸比无明显规律性差异;'夏黑'葡萄的可溶性固形物含量、固酸比明显增加;果实中酒石酸含量减少、L-苹果酸含量增加.综合分析可得,'户太8号'葡萄最佳处理为花期1~3 d浸蘸25 mg·L-1 GA3,10~15 d后再次浸蘸50 mg·L-1 GA3和2 mg·L-1 CPPU;'夏黑'葡萄最佳处理为盛花期浸蘸25 mg·L-1 GA3,10~15 d后再次浸蘸50 mg·L-1 GA3和2 mg·L-1 CPPU. 相似文献
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以东方百合'Siberia'为试材,在其栽培过程中施入鞘氨醇杆菌属(SN-2)、不动杆菌属(BG)、不动杆菌属(XG)、金黄杆菌属(F)4株含ACC脱氨酶的植物根际促生菌及上述菌株混合液,以清水为对照,测定其株高、叶绿素相对含量、茎粗、花期及花苞数等指标,探讨含ACC脱氨酶的PGPR对东方百合'Siberia'生长的影响,以期为菌肥的合理施用和开发提供参考依据.结果 表明:BG菌株对株高的促进作用最大,比对照高10.27%;F菌株对整株花期的延长作用最大,比对照高5.70%;SN-2菌株对单朵花期延长效果最佳,比对照高14.70%;XG菌株对减少乙烯释放量的效果最佳,比对照低29.63%;同时发现4株混合菌液对提高种球采收品质均有一定促进作用,其鲜质量、周径及根长分别比对照高54.44%、25.09%和48.79%.综合比较来看,BG菌株对'Siberia'的促生效果最佳. 相似文献
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食用百合种质的玻璃化法超低温保存技术初探 总被引:13,自引:1,他引:13
以离体茎尖为试材 ,采用玻璃化法 ,对食用百合离体超低温保存技术进行了初步研究。结果表明 ,用2~ 3mm茎尖 ,在MS +0 .5mol·L-1蔗糖浓度的培养基上预培养 1~ 2d ,室温下植物玻璃化溶液 (PVS2 )处理 2 0min ,换入新鲜的PVS2 ,迅速投入液氮中 ,2d后取出 ,在 4 0℃水浴中解冻 2min ,再在 2 5℃水浴中解冻 10min ,用1.2mol·L-1蔗糖液体培养基洗涤 2 0min ,接种在 6 -BA 0 .5mg·L-1+NAA 0 .1mg·L-1+GA3 0 .3mg·L-1+蔗糖 30 g·L-1+琼脂 7g·L-1的MS培养基上 ,2 5℃弱光培养 1周后转为正常光下培养 ,2周后再生率达到 5 2 .6 %。 相似文献
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以2种花色、花型良好,长势优异的雄性不育百合'5-4,与'5-35,为试材,采用组织培养法,研究不同消毒时间、激素浓度及不同基质配比对2种雄性不育百合组织培养过程中的影响,以期建立快速繁殖体系,为无花粉百合的大量生产提供参考依据.结果表明:最佳外植体灭菌方式为75%乙醇消毒20 s,10%NaClO处理8 min,和75%乙醇消毒30 s,10%NaClO处理6 min;最佳诱导培养基为 MS+NAA 1.0 mg·L-1+6-BA 0.5 mg·L-1和 MS+NAA 1.5 mg·L-1+6-BA 0.5 mg·L-1;最佳增殖培养基均为 MS+6-BA 1.5 mg·L-1+NAA 0.5 mg·L-1;最佳生根培养基为 1/2MS+IBA 0.1 mg·L-1+NAA 0.1 mg·L-1+蔗糖40 g·L-1 和 1/2MS+IBA 0.2 mg·L-1+NAA 0.1 mg·L-1+蔗糖40 g·L-1;最佳移栽基质比例为蛭石:珍珠岩:草炭=1 ∶1∶1. 相似文献
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《果树学报》2010,(6)
以西洋梨矮化砧木BA-29春季茎段为外植体,筛选适宜的消毒时间和诱导、继代增殖与生根培养基,并对试管苗进行了移栽。结果表明,(1)取春季田间嫩茎段为外植体,用0.1%HgCl2消毒5min,污染率可控制在20%以下,外植体成活率超过70%,效果优于消毒3min、7min和10min;以1/2MS+6-BA1mg·L-1+IBA0.1mg·L-1+GA0.2mg·L-1为诱导培养基,增殖倍数较高;(2)适宜继代增殖培养基为MS+6-BA1mg·L-1+IBA0.4mg·L-1+GA0.05mg·L-1,增殖系数可以达到3.8,且芽生长势强,新芽质量好;(3)适宜生根培养基为1/2MS+NAA0.5mg·L-1+IBA0.5mg·L-1,生根率81%,平均生根7.6条,最多达到10条;已生根试管苗炼苗7d后移栽于灭过菌的基质中,30d后移栽成活率超过80%。 相似文献
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GA3 +CPPU结合疏果处理对“夏黑”葡萄果实品质的影响 总被引:1,自引:0,他引:1
以5年生"夏黑"葡萄为试材,研究了赤霉素GA3(20mg·L~(-1))+不同浓度氯吡脲CPPU(1.0、3.0、5.0mg·L~(-1))处理与单穗不同留果量处理(单穗50、65、80粒)对"夏黑"葡萄果实品质(葡萄果穗及果粒性状、产量、可溶性固形物含量及总酸含量、总酚、单宁、总花色素含量)以及感官评价的影响。结果表明:连续2次对果穗浸蘸GA320mg·L~(-1)+CPPU 3.0mg·L~(-1),单穗留果量为65粒和80粒时均较对照显著使果粒增大、总酸含量降低,总花色素含量升高,同时果形指数未发生显著变化,而单穗留果量为65粒时果实可溶性固形物显著高于对照、单穗留果量为80粒时与对照没有显著差异;GA320mg·L~(-1)+CPPU 3.0mg·L~(-1)连续2次浸蘸果穗、每穗留果65粒,果实感官评价得分最高、口感最好。表明GA320mg·L~(-1)+CPPU 3.0mg·L~(-1)连续2次浸蘸果穗+单穗留果65粒的处理综合效果最好,而其它处理在不同方面存在一定缺陷。 相似文献
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以五年生葡萄品种‘阳光玫瑰’为试材,采用赤霉素(GA3)和氯吡脲(CPPU)搭配不同植物生长刺激物复合施用,研究了不同浓度处理对‘阳光玫瑰’果实品质的影响,以期筛选出适合‘阳光玫瑰’使用的植物生长调节剂组合配方。结果表明:GA3 25 mg·L-1+CPPU 2.5 mg·L-1处理可明显提高果实香味;GA3 25 mg·L-1+CPPU 5.0 mg·L-1处理果实无核率达到90%,显著提高果实的可溶性固形物及还原糖含量,但同时也伴随着果实空心现象增多;GA3 25 mg·L-1+CPPU 5.0 mg·L-1+壳寡糖800倍处理的果实无核化效果最好,并且空心率也较低。综合各指标,GA3 25 mg·L-1+CPPU 5.0 mg·L-1+壳寡糖800倍处理效果最好。 相似文献
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Effect of acupuncture on CD4+ CD25+ Foxp3+ regulatory T-cells in rats of embryo implantation failure
GAO Wei-na GUO Yue WANG Li-jun TANG Xiao LIU Ya-fei CHEN Zhen-yan ZHANG Ming-min HUANG Guang-ying 《园艺学报》2013,29(8):1464-1470
AIM:To observe the effect of acupuncture on CD4+ CD25+ Foxp3(forkhead box P3)+ regulatory T-cells(Treg cells) in rats with embryo implantation failure. METHODS:One hundred and forty-four pregnant rats were randomly divided into control group(N), mifepristone treatment group(M), mifepristone+acupuncture treatment group(A) and mifepristone+progestin treatment group(W). The rats in groups M, A and W were treated with mifepristone-sesame oil solution on day 1, while the rats in group N were injected with the same amount of sesame oil. The Housanli(ST36) and Sanyinjiao(SP6) points were selected for acupuncture. From day 1 to the time of death, the rats in group A were fasten up and then the acupuncture was performed. Accordingly, the rats in group N and group M were only fixed, and the rats in group W were given progestin. Implanted embryos in each group were counted. The proportions of CD4+ CD25+ Foxp3+ Treg cells in peripheral blood and CD4+ Foxp3+ Treg cells in the endometrium were detected by flow cytometry. The mRNA and protein levels of Foxp3 were determined by real-time PCR and Western blotting, respectively. RESULTS:Compared with group N, the number of implanted embryos, the percentages of CD4+ CD25+ Foxp3+ Treg cells in peripheral blood and CD4+ Foxp3+ Treg cells in the endometrium, and the expression of Foxp3 protein and mRNA in the endometrium were significantly decreased in group M(P<005). Compared with group M, the above indexes in group A and group W were significantly increased. CONCLUSION: The effect of acupuncture in rats with embryo implantation failure may be closely correlated with the modulation of CD4+ CD25+ Foxp3+ Treg cells. 相似文献
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以自根和嫁接黄瓜幼苗为试材, 研究叶片Mn、Cu、Zn和Fe含量及低温胁迫下SOD活性的差异, 结果表明: 嫁接黄瓜叶片Mn、Cu和Zn的含量均显著高于自根黄瓜, 而Fe的含量则显著低于自根黄瓜; 低温胁迫第3天, 嫁接黄瓜叶片SOD、Cu /Zn-SOD和Mn-SOD活性均显著高于自根黄瓜, Fe-SOD则显著低于自根黄瓜。为探索嫁接黄瓜叶片高含量Mn、Cu、Zn与SOD活性之间的关系, 向低温胁迫第3天的自根黄瓜叶片SOD 提取液中加入Mn2+ 、Cu2+和Zn2+ , 使之与嫁接黄瓜叶片SOD 提取液对应组合的Mn2+ 、Cu2+和Zn2+浓度相等, 研究此时的SOD及其同工酶活性的变化, 结果发现: Mn2+ 、Cu2+和Zn2+加入自根黄瓜叶片提取液后均能显著增强SOD活性, 其增强作用的大小依次为Mn2+ +Zn2+, Mn2+ + Cu2+,Mn2+, Cu2+, Cu2+ + Zn2+ +Mn2+, Cu2+ + Zn2+和Zn2+ 。3种离子的加入都不影响Fe-SOD的活性。这一结果说明黄瓜叶片Mn、Cu和Zn的含量是影响SOD活性大小的重要因素, 嫁接黄瓜吸收Mn、Cu和Zn的能力较强是其SOD活性显著高于自根黄瓜的重要原因之一。 相似文献
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AIM: To check the physical interaction between GST- Na+-K+-ATPase domain and recombinant human augmenter of liver regeneration (rhALR) by GST pull down assay. METHODS: With PCR and genetic recombinant techniques, the coding region of β subunit of Na+-K+-ATPase was cloned into expressing plasmid pGEX-4T and identified by endonuclease digestion and sequencing methods. Under the inducing of 0.1 mmol/L IPTG, the fusion protein GST- Na+-K+-ATPase domain was highly expressed by E.coli DH-5α. After hypersound quassating, the GST- Na+-K+-ATPase domain was purified by glutathione agarose beads and the physical interaction with rhALR was checked by GST pull down assay. RESULTS: Analysis by SDS-PAGE showed the rhALRs of monomer and dimmer in GST- Na+-K+-ATPase domain lane. The Western blotting of the GST-pull down assay showed the same results as well. CONCLUSION: The Na+-K+-ATPase domain is associated with rhALR specifically in vitro. 相似文献
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以盆栽枳椇的1 a生实生苗为试材,分析了在不同的盐处理0、0.15%、0.3%、0.45%、0.6%下对其根、茎、叶的K+、Na+离子含量和Na+/K+的影响。结果表明:随着盐浓度的增加,植物叶片中Na+含量和Na+/K+明显升高,说明枳椇没有将Na+截留在根、茎中,而较多的运输到了叶片,使枳椇受害较重。 相似文献
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以单季茭白品种蒋墅茭和双季茭白品种葑红早为试材,采用土壤为栽培介质,对Cd2+,Pb2+的单一及其复合胁迫,进行有机肥和无机肥处理,测定了茭白根、短缩茎、叶及产品器官中Cd2+.Pb2+的积累量.研究结果表明,Cd22+,Pb2+的积累量在茭白不同器官间表现为根>短缩茎>叶>肉质茎;两品种中复合胁迫时的Cd2+.Pb2+积累量均明显高于单一胁迫;不同肥料间以有机肥处理下茭白各器官中Cd2+.Pb2+的积累量同比均显著低于无机肥处理.品种问存在明显差异,蒋墅茭对Pb2+的积累能力强于葑红早,对Cd2+的积累能力则低于葑红早.茭白根系对Cd2+,Pb2+的富集系数表现为无机肥处理和复合胁迫处理时分别显著大于有机肥处理和单一胁迫. 相似文献
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采用19个不同类型南瓜品种,研究了300 mmol/L NaCl下,幼苗地上部和根系Na+和Ca2+离子含量和Na+/Ca2+比值的变化。结果表明:NaCl胁迫8 d后,不同南瓜品种幼苗Na+含量均明显增加,离子平衡被打破;南瓜幼苗体内的Na+含量、地上部的Na+/Ca2+比值的变化反映植物对盐离子和营养元素相对的吸收情况;青栗(Q1)南瓜幼苗根系Na+含量和Na+/Ca2+比值明显高于黑蜜南瓜(H2)和黑籽南瓜(H3)。由此可知:不同南瓜品种幼苗体内Na+含量、地上部Na+/Ca2+比值变化趋势与NaCI胁迫下不同南瓜品种幼苗的盐害指数的结果基本一致;进一步验证了Q1耐盐性强与NaCl胁迫下地上部Na+/Ca2+比值较低,Ca2+离子含量较高有关;而品种H2和H3对盐敏感与NaCl胁迫下地上部Na+/Ca2+比值较高,Ca2+离子含量较低有关。 相似文献
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柑橘果实总钙、可溶性Ca~(2+)含量及Ca~(2+)-ATPase活性的季节性变化 总被引:9,自引:1,他引:9
以单性结实的龟井和自花结实的鄂柑1号柑橘品种为材料,对果实的整个发育期的子房(幼果)、果皮和果肉的总钙、可溶性Ca2+含量和Ca2+-ATPase活性进行了测定。结果表明龟井花前至花期子房总钙含量就已很高,花后趋下降,而鄂柑1号花前至花期子房总钙含量相对较低,花后有一显著上升;龟井可溶性Ca2+含量在花期及花后有一明显下降,而对应鄂柑1号在花后却有一显著上升;龟井花前至花期Ca2+-ATPase活性较高,花后下降,而对应鄂柑1号花前较低,花期即始上升;果实增大期内两品种果皮的总钙均趋上升,对应果肉总钙却趋下降;而果皮和果肉的可溶性Ca2+含量和Ca2+-ATPase活性在增大期内均有一明显增长过程或居相对较高水平。 相似文献
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AIM:To investigate the proliferation of CD4+CD25+ T cells from PBMCs of the gastric cancer patients and the inhibitory effect on CD4+CD25- T cells in vitro. METHODS:Magnetic activated cell sorting (MACS) method was used to separate CD4+CD25+T and CD4+CD25-T cells from peripheral blood monocytic lymphocytes in the gastric cancer patients, and then the purity and activity of CD4+CD25+T cells were analyzed with flow cytometer. After stimulated with anti-CD3 Ab, anti-CD28 Ab and rh IL-2, CD4+CD25- and CD4+CD25+ T cells were cocultured. The inhibitory effect of CD4+CD25+T on CD4+CD25-T cells was assayed by [3H] thymidine proliferation experiment. RESULTS:(1)After sorting, CD4+CD25+ T cells purity in healthy control and gastric cancer patients were 83.80%±1.84% and 84.13%±2.77%, respectively. No significant difference between the two groups (P>0.05) was observed. (2)The activity of CD4+CD25+ and CD4+CD25- T cells in healthy control and the gastric cancer patients after sorting were 98.52%±0.72% and 97.80%±0.95%. There was no significantly difference between the two groups (P>0.05). (3) CD4+CD25+ T cells obviously inhibited the CD4+CD25-T cell proliferation in vitro. The inhibition achieved to maximum in coculture of CD4+CD25+ T cells together with CD4+CD25- T cells (ratio of 1∶〖KG-*2〗1). CONCLUSION:The MACS system can effectively isolate CD4+CD25+ and CD4+CD25- T cells. After sorting, CD4+CD25+T cells obviously inhibit the proliferation of CD4+CD25- T cells in vitro and the inhibitory effect display an effect-target ratio relationship. 相似文献