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1.
During the myotomal myogenesis in pike (Esox lucius) two phases of muscle differentiation can be distinguished. In the first phase, the somite cells-derived stock, the primary myoblasts (of mesodermal origin), fuse to form multinucleate myotubes. Participation of myotomal cells of mesodermal origin is insufficient for further muscle development. In the second stage mesenchymal cells migrate, via myosepts, into the myotome between myotubes. Immunocytochemical detection of proliferating cell nuclear antigen (marker of S phase of cell cycle) showed their mitotic activity. Transmission electron microscope analysis revealed that the differentiation of these cells depends on their position. Cells remaining in the myosepts develop into fibroblasts and produce collagen fibres, while those that have migrated into the myotomes transform into secondary myoblasts. Mesenchymal cells in the studied species are believed to participate in hypertrophy and hyperplasy of muscle fibres. Thus the muscle fibres in pike (E. lucius) are of mesodermal-mesenchymal origin. 相似文献
2.
In Coregonus lavaretus, prior the mesoderm segmentation, in cells adjacent to the notochord called adaxial cells MyoD and slow myosin heavy chain (MyHC‐slow) proteins were observed. After somite formation, adaxial cells migrate towards the lateral part of the myotome and form a layer of red muscles. Deeper cells differentiate into white muscle fibres. In situ hybridization using Pax‐3 molecular probe revealed, that after somitogenesis, Pax‐3 is expressed in a layer of cells superficial to the myotome resembling the “external cells” (found in many teleosts species) or dermomyotome described in Amniota. During later developmental stages Pax‐3 gene is expressed in cells in intermyotomal space and then in myoblasts between myotubes. In these cells Pax‐7 protein was also observed. Pax‐3/7 positive cells which have migrated into the myotomes differentiate into satellite cells/secondary myoblasts and participate in hypertrophic and hyperplastic growth of muscles. 相似文献
3.
Yamanouchi K Hosoyama T Murakami Y Nishihara M 《The Journal of reproduction and development》2007,53(1):51-58
The aims of the present study were to establish a culture system for goat skeletal muscle stem cells and to examine their myogenic and adipogenic properties in vitro. Cells were isolated from the skeletal muscle of the Shiba goat and cultured in vitro. Most of the cells were positive for myogenic markers, such as Pax7, MyoD, and desmin, and immunocytochemistry revealed they differentiated to form myotubes expressing myosin heavy chain, indicating they were highly myogenic. Myogenic differentiation was strongly suppressed by the addition of basic fibroblast growth factor, while proliferation was unaffected. When the cells were cultured in adipogenic differentiation medium, some of the cells differentiated into mature adipocytes that stained with Oil Red-O. These cells were immunocytochemically positive for adipogenic markers, including peroxisome proliferator-activated receptor-gamma (PPAR gamma) and CCAAT/enhancer-binding protein-alpha (C/EBP alpha). These results clearly demonstrate the presence of both myogenic and adipogenic stem cells in goat skeletal muscle. 相似文献
4.
O. Lógpez-Albors F. Gil G. Ramirez-Zarzosa J. M. Vázquez R. Latorre A. Garcia-Alcázar A. Arencibia F. Moreno 《Anatomia, histologia, embryologia》1998,27(4):223-229
The histochemical profiles - mATPase and NADH-TR reactions - of the red and white muscle fibres of gilthead sea bream and sea bass were determined from the first week after hatching. Modifications of the mATPase technique by combinations of pH/time/molarity were carried out in order to compare the sensitivity of the myosin ATPase of each muscle fibre type of the lateral muscle. Results showed that the staining of muscle fibres was independent of small modifications in the technique. The intermediate ‘pink’ muscle was histochemically defined towards the end of the larval life and is considered to be implicated in the growth of the myotome. A layer of external cells was observed, by electron microscopical examination, between the connective tissue of the skin and the superficial red muscle fibres of larvae and postlarvae. It is suggested that the external cells are unlikely to be a source of red muscle fibres and implicated on the growth of the myotome, but rather a part of the dermatome. The timing, areas and mechanisms of hyperplastic growth of the myotome were defined and discussed. 相似文献
5.
Serial sections of seventy four bovine embryos from 18 through 38 days gestation were studied to determine the processes and sequence of somite development and differentiation. Somites are formed from day 20 through day 32 or 33 depending upon the individual. Each newly formed somite consists of a discrete block of cells organized into a densely aggregated outer layer enclosing a core of loose mesenchymal cells. Each somite differentiates about 12 hours after its formation into sclerotome and dermamyotome. The ventral and medial surface layer becomes loosely mesenchymal continuous with the mesenchymal core while the rest of the surface layer remains unchanged as dermamyotome. Further change occurs during the next 24 hours as the dermamyotome flattens with somite growth to form a distinct surface layer of perpendicularly arranged cells, the dermatome, and an inner layer of longitudinally arranged cells, the myotome. By day 4 after a somite is formed a transitory artifact, the so-called “myocoele”, is often produced during dehydration for sectioning. The shrinkage artifact occurs progressively caudal through the sacral region until the cellular density of the myotomes equals that of the dermatomes. Vertebral development begins ventro-medial to somite pairs 6 through 12 on day 23 as sclerotomic cells form a continuous band beneath the notochord. Band production continues caudad progressively. The sclerogenic band is transformed into a continuous sclerogenic tube around the notochord at first ventro-medial to somite pairs 15 through 20 and then progressively cranial to somite pair 6 and caudal to somite pair 25 during day 26 as the dorsolaterally attached sclerotomes develop dense caudal halves. During day 27, sclerotomic cells from the caudal dense sclerotome halves form perichordal rings within the sclerogenic tube of the cervival and thoracic regions alternating with the blastemal centra which become vascularized during day 28. Neural and transverse processes develop during day 29 from the caudal dense sclerotome halves connected to the perichordal rings and cranial portion of the centra. Sclerotomes of the cervical through sacral regions disappear by day 31. The blastemal centra become procartilaginous (new term) during day 32 through 34. Precartilage is produced in the ribs during day 33, cervical and thoracic neural processes during day 35, lumbar and sacral neural processes and cervical and lumbar transverse processes during day 36, and sacral transverse processes during day 37. 相似文献
6.
Yamanouchi K Soeta C Suzuki S Hasegawa T Naito K Tojo H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2000,62(11):1213-1216
In isolating skeletal muscle satellite cells, sometimes a problem is encountered in removing contaminating nonmyogenic cells. In the present study, we constructed a novel vector, pSKA-EGFP, which achieves the expression of enhanced green fluorescent protein (EGFP) exclusively in myogenic cells under the control of skeletal alpha-actin promoter when transfected to primary cultured cells from skeletal muscle. Cells from rat skeletal muscle positive for EGFP after transfecting with pSKA-EGFP were all positive for desmin and none of the nonmyogenic cells expressed EGFP, indicating that the expression of EGFP is specific to myogenic cells. Among the cells positive for EGFP were proliferating cells, presumably satellite cells. In addition, EGFP positive cells derived from horse skeletal muscle after transfecting pSKA-EGFP in vitro formed multinuclear myotubes, indicating that myogenic expression of EGFP driven by skeletal alpha-actin was achieved also in the equine cells. These results indicated that pSKA-EGFP vector will be useful in identifying and following up the satellite cells in real time, and also permit us to isolate satellite cells in combination with fluorescence-activated cell sorting (FACS). 相似文献
7.
G. Ramírez-Zarzosa F. Gil J. M. Vázquez A. Arencibia R. Latorre O. López-Albors A. Ortega F. Moreno 《Anatomia, histologia, embryologia》1998,27(1):21-29
Fibre-type differentiation of lateral musculature has been studied in gilthead sea bream Sparus aurata (L.) and sea bass Dicentrarchus labrax (L.) during post-larval development using ultrastructural, histochemical and morphometric techniques. The study showed three muscle layers: red, intermediate (or pink) and white. Initially, most of the red muscle showed low myosin ATPase (m-ATPase) activity fibres, whereas near the transverse septum some small high m-ATPase activity fibres appeared and later acquired a rosette aspect. Afterwards, during adult growth the red muscle showed a histochemical mosaic appearance. The pink muscle in sea bass was observed at the beginning of juvenile development by the oxidative technique (NADH-RT reaction) whereas in gilthead sea bream it was also observed at the end of larval development. The pink layer consists of high m-ATPase activity fibres. However, along the muscle development other low and moderate m-ATPase activity fibres were observed close to the red and white muscles, respectively. The white muscle of juvenile fish showed a histochemical mosaic appearance near the pink muscle. In adult specimens the mosaic white muscle spread out occupying the whole of the myotome. Morphometric analysis shows a significant increase in mean fibre diameter during post-larval development, as shown by the Student's t-test (hypertrophic growth). Skewness and kurtosis values of fibre diameters point to the generation of a new fibres from the myosatellite cells (hyperplastic growth). 相似文献
8.
Transforming growth factor-beta1 (TGF-beta1) is a potent inhibitor of muscle cell proliferation and differentiation. Decorin, a small proteoglycan in the extracellular matrix, binds to TGF-beta1 and modulates the activity of TGF-beta1 during muscle cell growth and development. However, its interaction with TGF-beta1 and involvement in myogenesis is not well characterized. In the present study, chicken myogenic satellite cells, myogenic precursors for muscle growth and repair, were isolated from the pectoralis major muscle and used to investigate the biological function of TGF-beta1 and decorin during myogenesis. The over-expression of decorin in satellite cells significantly increased cell proliferation, compared to the control cells. Consistent with this result, reducing decorin expression decreased cell proliferation, which suggests a decorin-mediated mechanism is involved in the regulation of myogenic satellite cell proliferation. Satellite cells over-expressing decorin were less sensitive to TGF-beta1 during proliferation, which indicates that decorin may sequester TGF-beta1 leading to increased proliferation. During satellite cell differentiation, the over-expression of decorin induced differentiation by increasing the muscle specific creatine kinase concentration. However, the addition of TGF-beta1 diminished decorin-mediated cell responsiveness to TGF-beta1 during differentiation. Taken together, these results suggest that decorin induces myogenic satellite cell proliferation and differentiation by regulating cellular responsiveness to TGF-beta1. An alternative TGF-beta1-independent pathway may be involved in the regulation of satellite cells by decorin. 相似文献
9.
为研究肌肉生长抑制素（myostatin,MSTN）对牛骨骼肌卫星细胞增殖与成肌分化的影响,本试验以牛骨骼肌卫星细胞体外诱导成肌分化模型为对象,以前期设计合成3个干扰RNA（si-MSTN-1、si-MSTN-2、si-MSTN-3）并对其进行干扰效果筛选为基础,将干扰效果极显著的si-MSTN-2（si-MSTN）转染牛骨骼肌卫星细胞,通过EdU染色法检测干扰MSTN对牛骨骼肌卫星细胞增殖的影响;进一步对干扰MSTN的牛骨骼肌卫星细胞进行体外成肌诱导分化,通过肌管形成状态和分化标志因子综合分析干扰MSTN对牛骨骼肌卫星细胞分化的影响：首先通过显微镜观察牛骨骼肌卫星细胞分化时期的肌管形成状态,然后利用实时荧光定量PCR和Western blotting技术检测牛骨骼肌卫星细胞分化标志因子MyoG和MyHC在mRNA和蛋白水平的表达情况。结果显示,干扰MSTN后,牛骨骼肌卫星细胞中EdU阳性细胞率极显著增加（P < 0.01）,说明下调MSTN表达极显著促进了牛骨骼肌卫星细胞的增殖;牛骨骼肌卫星细胞诱导分化后形成的肌管数量和直径均呈现增大趋势,牛骨骼肌卫星细胞成肌分化标志因子MyHC在mRNA和蛋白水平的表达均极显著高于对照组（P < 0.01）,说明下调MSTN表达能够促进牛骨骼肌卫星细胞的成肌分化过程。本研究结果表明,干扰MSTN可以显著促进牛骨骼肌卫星细胞的增殖及成肌分化过程。本试验结果为进一步开展MSTN对牛骨骼肌卫星细胞成肌分化的调控机制研究提供了参考。 相似文献
10.
旨在探究肌球蛋白结合蛋白C1（myosin binding protein C1,MyBPC1）对牛骨骼肌卫星细胞增殖与成肌分化的影响,为进一步研究MyBPC1在细胞分化和肌肉发育过程中的调控作用提供依据。本研究利用西门塔尔胎牛原代牛骨骼肌卫星细胞体外诱导成肌分化模型模拟牛骨骼肌的生长发育过程。采用qRT-PCR和Western blot检测MyBPC1的细胞时序表达谱。试验分为两组。在RNA水平每组4个重复,每个重复20 μL;在蛋白水平每组3个重复,每个重复15 μg。采用qRT-PCR和Western blot检测牛骨骼肌卫星细胞转染MyBPC1的过表达效果,并进一步检测细胞增殖期标志因子Pax7、Ki67以及细胞分化期标志因子MyHC、MyOG的表达变化情况,观察牛骨骼肌卫星细胞肌管形成状态。结果,MyBPC1在牛骨骼肌卫星细胞分化前后表达水平存在极显著差异,牛骨骼肌卫星细胞诱导分化后MyBPC1的mRNA和蛋白表达量均极显著高于增殖期（P<0.01）。过表达MyBPC1后,细胞分化形成的肌管数量明显多于对照组,增殖标志因子Pax7的mRNA水平和蛋白表达水平无显著差异,分化标志因子MyHC的mRNA水平和蛋白表达水平极显著高于对照组（P<0.01）。过表达MyBPC1可以促进牛骨骼肌卫星细胞体外成肌分化,为进一步开展MyBPC1对牛骨骼肌卫星细胞的调控机制奠定基础。 相似文献
11.
为探究肌肉生长抑制素(MSTN)对牛骨骼肌生长发育的作用机制,本研究以前期MSTN^+/-蒙古牛与野生蒙古牛腿臀肌肌肉组织定量蛋白质组学与磷酸化蛋白质组学筛选获得的表达差异倍数较大的核心蛋白聚糖(DCN)为靶标,以实验室前期分离培养的牛骨骼肌卫星细胞及建立的体外诱导成肌分化模型为对象,通过对设计合成的3个DCN siRNA干扰效果的筛选,将干扰效果最显著的si-DCN-2(si-DCN)转染牛骨骼肌卫星细胞。采用实时荧光定量PCR和Western blotting方法检测增殖期(GM)牛骨骼肌卫星细胞中增殖标志因子Pax7和MyoD的mRNA水平及蛋白水平的表达变化,以及使用EdU染色的方法检测干扰DCN对细胞增殖的影响。对转染DCN siRNA的牛骨骼肌卫星细胞进行体外成肌诱导分化,通过显微镜观察牛骨骼肌卫星细胞分化第3天(DM3)的肌管形成状态,同时采用实时荧光定量PCR和Western blotting检测分化标志因子MyoG和MyHC的mRNA水平及蛋白水平的表达变化,并对DM3期肌管MyHC进行免疫荧光染色,以研究干扰DCN对细胞分化的影响。结果显示,干扰DCN表达后,增殖期牛骨骼肌卫星细胞中Pax7和MyoD的mRNA水平及蛋白水平都显著或极显著上调(P<0.05;P<0.01),且EdU阳性细胞率显著增加(P<0.05),表明干扰DCN表达显著促进了牛骨骼肌卫星细胞的增殖。干扰DCN表达后,牛骨骼肌卫星细胞分化第3天诱导形成的肌管直径呈现增大趋势,检测成肌分化标志因子MyoG在mRNA和蛋白水平的表达分别极显著和显著高于对照组(P<0.01;P<0.05),MyHC在mRNA水平显著降低(P<0.05),但在蛋白水平上极显著升高(P<0.01),免疫荧光结果显示,下调DCN后肌管融合指数显著高于对照组(P<0.05),说明干扰DCN表达能够促进牛骨骼肌卫星细胞的成肌分化过程。本研究结果表明,干扰DCN可以显著促进牛骨骼肌卫星细胞的增殖和成肌分化过程。研究结果为进一步开展MSTN对牛骨骼肌卫星细胞成肌分化的调控机制研究奠定了基础。 相似文献
12.
为探究肌肉生长抑制素（MSTN）对牛骨骼肌生长发育的作用机制,本研究以前期MSTN+/-蒙古牛与野生蒙古牛腿臀肌肌肉组织定量蛋白质组学与磷酸化蛋白质组学筛选获得的表达差异倍数较大的核心蛋白聚糖（DCN）为靶标,以实验室前期分离培养的牛骨骼肌卫星细胞及建立的体外诱导成肌分化模型为对象,通过对设计合成的3个DCN siRNA干扰效果的筛选,将干扰效果最显著的si-DCN-2（si-DCN）转染牛骨骼肌卫星细胞。采用实时荧光定量PCR和Western blotting方法检测增殖期（GM）牛骨骼肌卫星细胞中增殖标志因子Pax7和MyoD的mRNA水平及蛋白水平的表达变化,以及使用EdU染色的方法检测干扰DCN对细胞增殖的影响。对转染DCN siRNA的牛骨骼肌卫星细胞进行体外成肌诱导分化,通过显微镜观察牛骨骼肌卫星细胞分化第3天（DM3）的肌管形成状态,同时采用实时荧光定量PCR和Western blotting检测分化标志因子MyoG和MyHC的mRNA水平及蛋白水平的表达变化,并对DM3期肌管MyHC进行免疫荧光染色,以研究干扰DCN对细胞分化的影响。结果显示,干扰DCN表达后,增殖期牛骨骼肌卫星细胞中Pax7和MyoD的mRNA水平及蛋白水平都显著或极显著上调（P<0.05;P<0.01）,且EdU阳性细胞率显著增加（P<0.05）,表明干扰DCN表达显著促进了牛骨骼肌卫星细胞的增殖。干扰DCN表达后,牛骨骼肌卫星细胞分化第3天诱导形成的肌管直径呈现增大趋势,检测成肌分化标志因子MyoG在mRNA和蛋白水平的表达分别极显著和显著高于对照组（P<0.01;P<0.05）,MyHC在mRNA水平显著降低（P<0.05）,但在蛋白水平上极显著升高（P<0.01）,免疫荧光结果显示,下调DCN后肌管融合指数显著高于对照组（P<0.05）,说明干扰DCN表达能够促进牛骨骼肌卫星细胞的成肌分化过程。本研究结果表明,干扰DCN可以显著促进牛骨骼肌卫星细胞的增殖和成肌分化过程。研究结果为进一步开展MSTN对牛骨骼肌卫星细胞成肌分化的调控机制研究奠定了基础。 相似文献
13.
Satellite cells activity contributes to postnatal muscle growth. Herein, we have studied the respective influence of insulin and triiodothyronine (T3) on the proliferation and differentiation of primary bovine satellite cells isolated from Semitendinosus muscle of Montbéliard steers. Under basal conditions, satellite cells proliferated until the fifth day of culture, began to fuse into myotubes and expressed differentiation markers such as connectin, myogenin, and myosin heavy chain (MHC) isoforms. Insulin behaved as an effective mitogen. Moreover, it promoted extensive myotube formation and enhanced differentiation as shown by an increase in the accumulation of differentiation markers. Maximal differentiation occurred with insulin physiological range concentrations. A delay in the stimulation of differentiation was registered with a high dose that promoted maximal proliferation. Conversely, T3 decreased cell proliferation in a dose-dependent manner. In addition, fusion and biochemical differentiation (accumulation of connectin, MyoD1, myogenin, and myosin heavy chain isoforms) were also enhanced. Bovine satellite cells seemed to respond differentially to insulin and T3 for proliferation. Interestingly, both hormones displayed a myogenic influence. Our observations suggest that both hormones could influence bovine satellite cells in vivo and contribute to the regulation of postnatal muscle growth. 相似文献
14.
Insulin-like growth factor binding proteins (IGFBPs) have been shown to affect proliferation of several cell types via insulin-like growth factor (IGF)-dependent and IGF-independent mechanisms. The goal of this study was to determine if levels of IGFBP-2, -3, -4 and -5 mRNA changed during differentiation of cultured porcine embryonic myogenic cells. Total RNA was isolated from muscle cultures at various stages of differentiation and Northern blots of this RNA were probed with 32P-labeled cDNA probes specific for individual IGFBPs. Fusion, myogenin mRNA, and creatine phosphokinase activity were used as markers of differentiation. The level of IGFBP-3 mRNA in differentiating cultures (120 h in culture) was only one-third of the level in myogenin negative, nonfused cultures (72 h in culture) (P < 0.05, n = 4). In contrast, the level of IGFBP-3 mRNA in extensively fused cultures (144 h in culture) was increased by three-fold as compared to the level in myogenin negative, nonfused cultures (P < 0.05, n = 4) and approximately seven-fold as compared to the 120-h cultures (P < 0.05, n = 4). No significant change in the level of IGFBP-5 mRNA was observed during differentiation of myogenic cultures. IGFBP-2 mRNA levels were not significantly different at 72, 96 and 120 h, but at 144 h IGFBP-2 mRNA level was increased three-fold as compared to nonfused cultures (72 h) (P < 0.05, n = 4). IGFBP-4 mRNA was not detectable on Northern blots of total RNA from porcine myogenic cultures at any stage of differentiation. Changes in IGFBP-3 and IGFBP-2 mRNA levels are associated with differentiation of embryonic porcine myogenic cells in culture and this may indicate that these IGFBPs play a role in differentiation of these cells. 相似文献
15.
Expression of myogenic regulating factors, Myogenin and MyoD, in two canine botryoid rhabdomyosarcomas 总被引：1，自引：0，他引：1
Kobayashi M Sakai H Hirata A Yonemaru K Yanai T Watanabe K Yamazoe K Kudo T Masegi T 《Veterinary pathology》2004,41(3):275-277
Myogenin and MyoD regulate the development of skeletal muscle, and their expressions are specific to the stages of myogenesis. Therefore, these myogenic regulatory proteins could be considered as sensitive and specific markers for rhabdomyosarcoma. In this report we investigated the immunohistochemical reactivities of myogenin and MyoD in two canine bladder botryoid rhabdomyosarcomas that were different in the degree of differentiation. MyoD was stained in the Ki-67 antigen-positive undifferentiated mesenchymal cells, which had proliferative activity similar to myoblasts differentiated from mesoblasts. In contrast, multinucleated neoplastic cells were positive for myogenin and alpha-sarcomeric actin but not for Ki-67 antigen, similar to the myotubes differentiated from myoblastic cells. The expressions of myogenin and MyoD were closely correlated to the histologic features of myogenic neoplastic cells. 相似文献
16.
The oviduct has an important role in regulating transport of gametes and fertilization. The main role in these functions has a smooth muscle cells and ciliated epithelium lining the oviduct. All functions are under the influence of hormonal and nervous system. The objective of this study was immunohistochemically to examine the following structures: lining epithelium, smooth muscle cells, elastic fibres and nerve fibres. For this purpose, the following antibodies were used: cytokeratin 18, S‐100 protein, acetylated α‐tubulin, smooth muscle actin, desmin and elastin. Ciliary and secretory cells of the lining epithelium were positive for cytokeratin 18 and S‐100 protein. Cilia and the basal body‐associated structures of ciliary cells were positive to acetylated α‐tubulin. Smooth muscle cells (SMC) in mucosa and of the muscular layer were positive for α‐smooth muscle actin (SMA) and desmin. High density of nerve fibres positively reacted to acetylated α‐tubulin and S100 protein was present in the mucosa, muscular layer and serosa. Elastic fibres positive for elastin form a dense network at the base of the mucosal folds and in the muscle layer. A dense network of these fibres is accompanying the blood vessels. It is supposed that together with smooth muscle cells they are involved in the transport of ovum and in blood flow regulation. 相似文献
17.
18.
R.P. Rhoads M.E. Fernyhough X. Liu D.C. McFarland S.G. Velleman G.J. Hausman M.V. Dodson 《Domestic animal endocrinology》2009
The existence of myogenic satellite cells was reported some 47 years ago, and, since that time, satellite cell research has flourished. So much new information is generated (daily) on these cells that it can be difficult for individuals to keep abreast of important issues related to their activation and proliferation, the modulation of the activity of other cell types, the differentiation of the cells to facilitate normal skeletal muscle growth and development, or to the repair of damaged myofibers. The intent of this review is to summarize new information about the extrinsic regulation of myogenic satellite cells and to provide specific mechanisms involved in altering satellite cell physiology. Where possible, examples from agriculturally important animals are used for illustrative purposes. 相似文献
19.
Keisuke Suzuki Yasuhiro Kishioka Jun‐ichi Wakamatsu Takanori Nishimura 《Animal Science Journal》2013,84(9):669-674
Decorin, a small leucine‐rich proteoglycan, plays an important role in cellular activities through modification of growth factors. It also acts as a signaling molecule to non‐muscle cells through epidermal growth factor receptor or insulin‐like growth factor I receptor (IGF‐IR). However, it is unclear if decorin acts as a signaling molecule to myogenic cells. In this study, we investigated the effect of decorin on the differentiation of myoblasts and the signaling via IGF‐IR to myogenic cells. C2C12 myoblasts cultured in media containing decorin for 72 h showed more extensive formation of multinucleated myotubes than control cells cultured in the same media without decorin. The protein expressions of myogenin and myosin heavy chian were higher in decorn‐treated cells than in control cells. These results suggest that decorin enhances the differentiation of myoblasts. Western blot analysis and immunocytochemistry showed that IGF‐IR was expressed in myoblasts and myotubes. Furthermore, Akt, which is downstream of IGF‐IR, was more phosphorylated in myoblasts cultured in media containing decorin than those in media without decorin. These results suggest that decorin activates Akt downstream of IGF‐IR and enhances the differentiation of myogenic cells. 相似文献
20.
为了研究核不均一核糖核蛋白AB(heterogeneous nuclear ribonucleoprotein AB, HNRNPAB)对牛骨骼肌卫星细胞增殖与分化的影响。本研究以体外分离培养的鲁西黄牛胎牛原代骨骼肌卫星细胞为试验材料,体外诱导成肌分化,分别收取分化前和分化后第1、2、3天的细胞,提取RNA(每组设置4个重复)或蛋白质(每组设置3个重复),通过qRT-PCR、Western blot检测HNRNPAB在成肌分化前后的表达情况。随后合成HNRNPAB的干扰RNA,并转染牛骨骼肌卫星细胞干扰HNRNPAB的表达,设置阴性对照组;在试验组和对照组中,分别利用EdU试验检测细胞增殖情况,qRT-PCR技术、Western blot技术检测HNRNPAB、增殖标志因子Pax7、Cyclin D1及分化标志因子MyoG、MyHC的mRNA表达水平及蛋白表达水平。结果显示,HNRNPAB在牛骨骼肌卫星细胞成肌分化过程中的mRNA表达呈现先升后降的趋势,在分化第1天表达量最高,分化前后蛋白表达水平也具有显著差异。干扰HNRNPAB后,EdU细胞阳性率极显著上升(P<0.01),增... 相似文献