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探讨活性灵芝三萜对各种癌细胞的生长抑制和诱导凋亡作用。采用MTT观察活性三萜对癌细胞的生长抑制作用,采用共聚焦扫描电镜和流式细胞术检测了该活性三萜对各种癌细胞的促凋亡作用。该活性灵芝三萜能够剂量依赖性地抑制各种癌细胞的增殖,并且在流式细胞术检测出现亚二倍体峰,共聚焦扫描电镜可观察到凋亡细胞。该活性灵芝三萜能够抑制各种癌细胞的生长并能诱导细胞发生凋亡。 相似文献
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For effecting of SOD on apoptotsis of arsenic compound induced, active oxygen is found to be involved in apoptotic progress affected by exogenous SOD and measurement of SOD in cell sap. Arsenic compounds cause a decrease of SOD in cell, promoting active oxygen to produce in mitochondria, ceil apoptosis and active oxygen to react with SOD. Quantititive SOD are ccpsume by arsenic compounds. It is exacdy the decrease that make plenty of active oxygen gather in mitccnondria, and starts the apoptotie progress. It is result that SOD protects, ceil in apoptosis. The analysis of SOD values shows that active oxygen has an effect on apoptosis. 相似文献
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In recent yuars,environmental arsenic contamination is a major problem in many parts of the world. There are many reports from many nations on human exposure to arsenic in drinking water has been linkedto various diseases and chronic arsenic poisoning symptoms . Though arsenic has been known as a poison for thousands of years, there have long been reports of benfical effects.Chinese medicine has used arsenic as a medicinal for thousands of years,especially studies have identified arsenic trioxide as a powerful and dramatic therapy for acute promyelocytic leukaemia(APL),which suggested many scholor use this drug to cure various tumors. 相似文献
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亚麻木酚素研究进展 总被引：8，自引：0，他引：8
综述了亚麻木酚素的结构、代谢、功能及应用方面的研究进展。亚麻木酚素具有抗肿瘤生成作用、雌激素及抗雌激素效应、抗癌、抗动脉硬化、预防糖尿病、抑制芳香酶活性、抑制DNA和RNA合成、抗病毒、抗真菌等多种功效。在食品、临床医学和化妆品领域应用广泛。随着人类生活水平的提高和营养的变化,心血管疾病、癌症、糖尿病等与饮食习惯有关的疾病迅速上升,木酚素对于预防与防治这一类疾病意义重大,同时化妆品也成为一大消费领域,以木酚素为主要原料的化妆品可以预防或治疗肌肤衰老。木酚素在亚麻籽中含量非常丰富,中国又是亚麻的生产大国,开发亚麻木酚素会产生巨大的经济和社会效益,前景广阔。 相似文献
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[Objectives]To explore the inhibitory effect of AURKB gene in apoptosis and cancer cell growth in HCT 116 cells.[Methods]The in vitro cytology studies were carr... 相似文献
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The presence of excessive amounts of heavy metals such as arsenic in the environment is a problem in many parts of the world. Many countries e.g., Bangladesh are already severely affected by arsenic contamination of soil and groundwater. Phytoaccumulation is a method in which plants are used to uptake heavy metals from soil. The genus Brassica has many species that can efficiently accumulate arsenic. Despite many studies on phytoaccumulation, little is known about the genetics of phytoaccumulation of Brassica. This study investigated the genetic effect on phytoaccumulation of arsenic in Brassica juncea L. and attempted to identify any morphological trait(s) for phytoaccumulation. Two cultivars of B. juncea namely, B. juncea cv. Rai and B. juncea cv. BARI-11 were chosen as parents for the study. A set of their morphological characteristics were selected to evaluate their potential as marker(s) for phytoaccumulation. A single concentration, 15 ppm of arsenic was used to study the genetic effect on phytoaccumulation using quantitative genetics. The results revealed that phytoaccumulation of arsenic (by the root and shoot systems) is heritable in the broad sense and polygenic in nature. The stem diameter was also found to be heritable in the broad sense and path analysis indicated that it has a significant direct effect on the shoot uptake. It is concluded that phytoaccumulation of arsenic in B. juncea (cvs. Rai and BARI-11) is a genetic phenomenon and stem diameter may be a potential marker of phytoaccumulation of arsenic in B. juncea (cvs. Rai and BARI-11). 相似文献
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台灣西南沿?貐^土壤中砷含量有偏高情形,砷含量過高會經由各種途徑對人體造成毒害。以X-射線繞射儀與傅立葉轉換紅外線吸收光譜儀證明普魯士藍對三價砷的孯恍晕綑C制,再以普魯士藍修飾電極搭配流動注入系統來偵測土壤三價砷,並探討堆肥對三價砷在不同土壤中吸附與脫附的影響。試驗結果得知電化學分析技術可快速、簡便且準確地偵測土壤三價砷,且結果中也指出堆肥處理會增加秀水與後里土壤對三價砷的吸附。此外,三種土壤不論有無施用堆肥,脫附百分率的大小順序爲興大>秀水>後里,其中秀水土壤雖在施用堆肥後吸附力大小明顯增加,但脫附百分率下降的情形較不明顯,顯示黏土礦物種類與含量控制三價砷的脫附行爲。 相似文献
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砷以有机态和无机态广泛存在于自然界中。国际癌症研究中心（IARC）将砷和无机砷化合物列为第一类致癌物（Group1,对人类致癌）。食物和水是无机砷暴露的主要途径。无机砷膳食暴露对人体健康的影响已受到广泛重视。文章综述了近年来联合国粮农组织和世界卫生组织食品添加剂联席专家委员会（JECFA）、欧洲食品安全局（EFSA）等国际组织对食品中砷安全性评价的最新进展,总结了目前中国关于砷膳食暴露的研究现状,指出食品中砷的形态、不同砷形态的毒性作用、生物有效性价态以及食品中无机砷的提取和检测方法将是砷安全性评价的重要研究方向。旨在为进一步开展砷的膳食暴露评估及其他相关研究提供参考。 相似文献
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To investigate the effects of human soluble TRAIL(sTRAIL) on cell growth and apoptosis of hepatocellular carcinoma cell line SMMC7721.After treatment of sTRAIL,the cell proliferation and apoptosis are determined by invertible microscope,trypan blue exclusion test,MTT assay,terminal deoxynucleotidly transferase mediated labeling(TUNEL)and DNA fragmentation assay.Chromatin condensation is observed by invertible microscope,Proliferation of SMMC7721 cell is significantly inhibited after the treatment with sTRAIL,TUNEL results show in comparison with control,the difference of the apoptosis index of cells treated by sTRAIL is significant,and DNA ladder is observed.The results indicate that sTRAIL can induce apoptosis of hepatocellular carcinoma cell line SMMC7721. 相似文献
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【研究目的】抗生素是某些微生物在生长代谢过程中产生的次级代谢产物,具有抑制或杀死微生物的能力[1]。根据抑菌带的长短,即可判断氨苄西林对不同细菌的影响以及不同浓度氨苄西林对同一种菌的影响,初步判断其抗菌谱。【方法】采用滤纸条法测定氨苄西林的抗菌谱,选用的菌种为金黄色葡萄球菌,大肠杆菌,枯草芽孢杆菌。【结果】随着氨苄西林浓度的增加,抑菌带的长度逐渐增长,抑菌效果越来越强,但同一浓度的氨苄西林对金黄色葡萄球菌,大肠杆菌,枯草芽孢杆菌的抑制效果差别不是很明显。【结论】氨苄西林通过抑制转肽作用使细菌的细胞壁解体而死亡。 相似文献
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从刺五加果实体中纯化得到一种均一分子量多糖ASPF,对其结构表征及体外抗肺癌活性进行研究。将提取得到的粗多糖通过DEAE-52柱层析分离得到3个组分,进行体外抗肺癌活性筛选后,将ASPB-3通过Sephadex G-200凝胶柱色谱得到ASPF,选择GPC、IR、HPLC方法对该多糖进行结构解析,并采用CCK-8法研究其对肺癌细胞体外存活率的影响。测得ASPF的糖醛酸含量为22.9%,是一种酸性果胶类结构多糖,其相对分子质量MW为9.7462×104,IR扫描结果显示其具有吡喃环结构,单糖测定结果显示其由鼠李糖、半乳糖醛酸、葡萄糖、木糖、半乳糖、阿拉伯糖6种单糖组成,对A549细胞存在抑制效果,其机制可能与作用于凋亡基因Bcl-2/Bax有关。ASPF对人肺癌细胞具有一定体外抑制作用。 相似文献
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黑果枸杞(Lycium ruthenicum Murry)中含有多种生物活性成分,如花青素类、多糖类、黄酮类、甜菜碱等,这些活性成分发挥抗肿瘤的药理作用机制目前已较为明确,其主要包括抑制肿瘤细胞增殖、诱导肿瘤细胞凋零、周期阻滞、肿瘤细胞分化及增强对肿瘤细胞免疫等作用。但目前关于黑果枸杞中主要抗肿瘤成分组成及对抑制肿瘤的转化机制和影响作用尚不明确。因此,综述了黑果枸杞中花青素、多糖、黄酮、甜菜碱等活性成分的研究现状及抗肿瘤机制,阐述了近年来有关黑果枸杞在抗肿瘤方面的研究进展,为黑果枸杞中功能性成分深入研究及精深加工提供参考依据。 相似文献
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Effect of ultrasonic on fermentation of microorganism makes cell membrane damage and has an increase of cell membrane permeability, and the membrane allows matter within to leave and that outside to enter the cell. When the power of ultrasonic is appropriately low, the cell can be retreated by themselves. Owning to this effect on fermentation, it can promote the cell growth, at the same time, can accelerate the secondary metabolite biosynthesize. As a result, the fermentation time is shortened and the production cost is reduced. This paper summarizes application of low ultrasonic in fermentation of microorganism and discusses its potential mechanism. 相似文献
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设置土壤加砷的大豆盆栽试验,测定大豆株高、结荚期与鼓粒期的叶绿素含量和大豆成熟收获后的生物量。结果表明,当土壤砷含量达50 mg kg–1时,大豆表现明显的中毒症状,植株矮化,叶色暗绿,叶片皱缩,成熟延迟。大豆株高随土壤加砷量增加而降低,高砷量也显著减少大豆生物量,加砷量达100 mg kg–1时,大豆株高、茎叶生物量、地上生物量、籽粒产量和总生物量分别下降45.0%、36.6%、44.6%、56.1%和43.4%。高砷量增加根系与地上、茎叶与地上生物量的比值,降低籽粒与地上、籽粒与茎叶、籽粒与总生物量的比值。土壤加砷量对大豆结荚期叶绿素含量无显著影响,但是高砷量显著降低大豆结荚期叶绿素a与叶绿素b的比值。土壤加砷量为50 mg kg–1和100 mg kg–1时,大豆鼓粒期的叶绿素a、叶绿素b、叶绿素总量和类胡萝卜素分别增加120.4%和96.1%、112.2%和91.5%、117.8%和94.5%、104.4%和83.7%,同时,结荚期与鼓粒期叶绿素含量的比值显著降低。由此可见,土壤中高砷含量对大豆植株有毒害作用,影响大豆生物量的分配,降低大豆地上生物量和籽粒产量,改变叶绿素的构成。相对增加生长后期叶绿素的含量可能是大豆成熟延迟的重要原因。 相似文献
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Being a toxic metalloid and group I carcinogen, Arsenic (As) poses a threat to plants, especially to crops which are consumed by human beings, and sooner or later results in hyper/hypopigmentation and skin cancer. It is a well‐known fact that South‐East Asia is suffering from groundwater As contamination, and according to a recent report, the contamination has been found also in Hungary, Mexico, Argentina, Australia, United States, etc. Thus, As contamination has become a global problem. As is toxic even at low concentration because it has no known function as nutrients. Arsenite (III) and arsenate (V) are the main phytoavailable forms of inorganic arsenic. Being analogous to phosphate, As(V) is transported by a phosphate‐cotransport system in plants, whereas As(III) is transported through ‘OsNIP2.1’ (member of aquaporin superfamily) in rice. Besides, ‘AsSe1’ (As‐accumulation gene), ‘AsTol’ (As‐tolerance gene) and ‘OsACR2.1’ (an arsenate reductase gene) have also been identified. The production of phytochelatins (PCs), a metal‐binding thiol peptide, in response to As stress may hold a way of proper As tolerance in plants but still needs a thorough study. However, with the proper knowledge of arsenic speciation, transportation, accumulation, overexpression in crop plants may result in ways to develop arsenic tolerant transgenics. 相似文献
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《Medicinal Plant》2014,(6)
Poria polysaccharide chemical components can combat tumor and cancer and boost the immune system. It also has antibacterial,antiviral,and anti-inflammatory effect. It has the function of anti-oxidation,and it can delay the age. It is able to protect liver,remove jaundice and promote sound sleep. It can also prevent and remove calculus. It is of the function of dropping glucose and anticoagulating. This paper reviews and analyses the reports and research of functions of Poria polysaccharide and provides references for its clinical application and research. 相似文献
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外源砷在3类土壤转化形态及其烟株中的分布 总被引：2，自引：1，他引：1
为探索外源砷在不同类型土壤中的形态转化状况以及对烟株吸收砷的影响,采用盆栽试验和化学分析相结合的方法,研究外源砷在红壤、紫色土、水稻土3种土壤中形态转化、分布特征以及烟株中砷的分布情况。研究结果表明,不同类型土壤中烟株对砷的吸附量具有显著差异性,说明土壤性质差异对烟株吸收富集砷具有较大影响。其中,红壤中烟株各个部位对砷的吸附率均最高,紫色土最低,说明砷在红壤中具有较高的活性。砷在烟株中的分布趋势为根＞下部叶＞中部叶＞上部叶＞茎。外源砷进入不同类型土壤后,残渣晶格态为主要赋存形态,与植物可吸收态比例相差不大,红壤中可交换态比例最高,紫色土最低;而紫色土碳酸盐结合态比例最高。各个形态分布在15天基本稳定,随着时间累积其他形态会有部分向残渣晶格态转移。 相似文献
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绣球菌多糖诱导K562、THP-1细胞凋亡的研究 总被引：1，自引：1，他引：0
本文的目的是研究绣球菌多糖对K562、THP-1细胞凋亡的诱导作用。利用MTT比色法测定绣球菌多糖对K562、THP-1细胞增殖的抑制作用;通过吖啶橙染色观察K562、THP-1细胞形态学上的改变;同时利用DNA琼脂糖凝胶电泳检测细胞凋亡的情况。实验结果表明：经绣球菌多糖处理后,K562、THP-1细胞的生长受到了显著抑制,最大抑制率分别为46.57%和59.82%;K562、THP-1细胞出现了典型的细胞凋亡形态学特征;同时,琼脂糖凝胶电泳呈现DNA梯形条带。由此说明,绣球菌多糖可在体外抑制K562、THP-1细胞生长并诱导其凋亡。 相似文献