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1.
The effect of trachyloban-19-oic acid isolated from Iostephane heterophylla (Asteraceae), was investigated on several photosynthetic activities in spinach thylakoids. The results indicated that this compound inhibited ATP synthesis and uncoupled electron transport, as well as basal and phosphorylating electron flow. Therefore, trachyloban-19-oic acid acts as Hill reaction inhibitor. This compound did not affect photosystem I activity but inhibited uncoupled photosystem II electron flow from H2O to DCPIP, and has not effect on electron flow from H2O to SiMo, indicating that the site of inhibition of this compound is at the level of QA-QB. Chlorophyll a fluorescence measurements confirm the behavior of this diterpene as QA-QB inhibitor, and in the other hand, this results indicate that a perturbation in the thylakoid membranes at the level of LHC II occurs.  相似文献   

2.
Biochemical effects of glyphosate have been examined with a variety of plant materials using mostly merislematic or actively growing tissues. The accumulation of chlorophyll was severely retarded and photosynthetic CO2 uptake was inhibited to a lesser extent. These inhibitory effects could not be alleviated by the simultaneous admixture of divalent cations. Glyphosate enhanced the initial substrate-induction of nitrate reductase, but repressed induction of nitrite reductase, in Ihe latter case correlating with both inhibition of chlorophyll accumulation and CO2 uptake. Inhibition of macromolecule synthesis in single node buds of Agropyron repens(L.) Beauv. was due partly to inhibition of 14C-precursor uptake. The specific activity of soluble acid phosphatase was enhanced as was the evolution of ethane. Ethylene production was not greatly affected. A marked decrease in microsomal protein was observed but the specific activities of several microsomal enzymes did not decline. Glyphosate had little inhibitory effect on the activity of microsomal ATPases in vitro.  相似文献   

3.
Cells were isolated from the developing leaves of Ipomoea aquatica and Digitaria sanguinalis. The effects of phenoxy alkanoic acid herbicides on light-dependent 14CO2 fixation and oxygen evolution in these leaf cells were studied. (2,4-Dichlorophenoxy)acetic acid and (2,4,5-trichlorophenoxy) acetic acid (2,4,5-T and 2,4-D) caused a 20% stimulation of 14CO2 fixation at 0.8 × 10−5M and an inhibition at 1 × 10−4M in I. aquatica leaf cells. Temperature seemed to have a marked influence on such action. No effect or very little effect was observed in the leaf cells of D. sanguinalis. The nonactive (2,4,6-Trichlorophenoxy)acetic acid (2,4,6-T) caused a similar stimulation of CO2 fixation as 2,4-D and 2,4,5-T at low concentrations in I. aquatica leaf cells, but no inhibition was observed at high concentration. Increase of hight intensity increased the rate of CO2 fixation in both control and 2,4,6-T-treated cells; however, the percentage of stimulation remained the same. At stimulatory concentration, all three compounds did not cause any stimulation in either photosystem I and II or photosystem II-mediated oxygen evolution. At higher concentrations, the differential effects of 2,4-D and 2,4,5-T on the light-induced CO2 fixation and photosystem II-mediated oxygen evolution in the I. aquatica leaf cells and D. sanguinalis mesophyll (ms) cells may be attributed in part to their selective action against dicotyledonous plants.  相似文献   

4.
The effect of chlorfenprop-methyl, flampropisopropyl and benzoylprop-ethyl on 14CO2 fixation was followed in wild oat (Avena fatua L.), barley (Hordeum vulgare L., cv. Ametyst), and wheat (Triticum aestivum L., cv. Mironovská). Experimental plants were exposed to a 14CO2-enriched atmosphere in a special apparatus 2 h, 1, 3, and 9 days after the herbicide treatment. Chlorfenprop-methyl already inhibited 14CO2 fixation in wild oat plants 2 h after the treatment. 14C-metabolite transport to the roots was strongly decreased. Both 14CO2 fixation and 14C-metabolite level in the roots were significantly depressed in A. fatua when compared with untreated plants at the last sampling time. 14C incorporation into starch was inhibited from the first day after treatment, and on day 9 was lowered more than ten fold in treated plants. Flamprop-isopropyl inhibited 14CO2 fixation in wild oat plants from day 3 after treatment, but benzoylprop-ethyl not until day 9. Both herbicides also decreased 14C incorporation into starch in A. fatua. Chlorfenprop-methyl also slightly decreased 14CO2 fixation in barley on day 9. However, assimilate transport into the roots and 14C incorporation into starch were not affected. Flamprop-isopropyl inhibited 14CO2 fixation in barley plants only on the first day after treatment, and assimilate transport was also reduced. By contrast, no differences from untreated plants were found at the end of the experiment. Benzoylprop-ethyl did not decrease either 14CO2 fixation or assimilate transport to the roots in wheat, but it inhibited starch synthesis. Atrazine depressed 14CO2 fixation in wild oat plants by 91%, in wheat plants by 99% compared with untreated plants. Assimilate transport into the roots was also strongly inhibited. In contrast to atrazine, the effect of chlorfenprop-methyl, flamprop-isopropyl, and benzoylprop-ethyl on CO2 fixation seems to be secondary.  相似文献   

5.
The electron transport inhibition, uncoupling, and binding of ioxynil and bromoxynil salts is compared in chloroplast fragments isolated from two weed species with contrasting responses to the hydroxybenzonitriles. Ioxynil Na was three to four times more inhibitory than bromoxynil K towards DCPIP and SiMo reduction in both Matricaria inodora and Viola arvensis. Ioxynil Na was also a more potent uncoupler of PSI-dependent electron transport from ascorbate/DCPIP to methyl viologen. Uncoupling occurred at concentrations higher than those that inhibited electron transport. Binding studies with [14C]bromoxynil K and [14C]ioxynil Na salts revealed slightly biphasic curves with no significant difference in the amounts of the two herbicides bound at a given concentration. The ratios of inhibition constant (Ki) and binding constant (Kb) were approximately one for ioxynil Na and three for bromoxynil K. Radiolabelled herbicide displacement studies revealed that ioxynil Na could partially displace bound [14C]bromoxynil K, but bromoxynil K could not displace bound ioxynil Na at biochemically active concentrations. Ioxynil Na may be a more effective inhibitor than bromoxynil K because it binds more strongly to the thylakoid membrane.  相似文献   

6.
Cells were isolated from the developing leaves of Ipomoea aquatica and Digitaria sanguinalis. The effects of phenoxy alkanoic acid herbicides on light-dependent 14CO2 fixation and oxygen evolution in these leaf cells were studied. (2,4-Dichlorophenoxy)acetic acid and (2,4,5-trichlorophenoxy) acetic acid (2,4,5-T and 2,4-D) caused a 20% stimulation of 14CO2 fixation at 0.8 × 10?5M and an inhibition at 1 × 10?4M in I. aquatica leaf cells. Temperature seemed to have a marked influence on such action. No effect or very little effect was observed in the leaf cells of D. sanguinalis. The nonactive (2,4,6-Trichlorophenoxy)acetic acid (2,4,6-T) caused a similar stimulation of CO2 fixation as 2,4-D and 2,4,5-T at low concentrations in I. aquatica leaf cells, but no inhibition was observed at high concentration. Increase of hight intensity increased the rate of CO2 fixation in both control and 2,4,6-T-treated cells; however, the percentage of stimulation remained the same. At stimulatory concentration, all three compounds did not cause any stimulation in either photosystem I and II or photosystem II-mediated oxygen evolution. At higher concentrations, the differential effects of 2,4-D and 2,4,5-T on the light-induced CO2 fixation and photosystem II-mediated oxygen evolution in the I. aquatica leaf cells and D. sanguinalis mesophyll (ms) cells may be attributed in part to their selective action against dicotyledonous plants.  相似文献   

7.
Cells were isolated from the developing leaves of Ipomoea aquatica (water spinach), a C3 plant, and three kinds of C4 plants, namely, Digitaria sanguinalis (NADP+-specific malate dehydrogenase type), Panicum miliaceum (NAD+-specific malic enzyme type), and Panicum texanum (phosphoenopyruvate carboxy kinase type), to study the effect of monuron on light-dependent 14CO2 fixation and oxygen evolution. Bundle sheath cells, except for those of D. sanguinalis, and mesophyll cells of all plants fixed approximately the same amount of 14CO2. Monuron, at the range used (2 to 10 × 10?7M), showed strong inhibition in the mesophyll cells of water spinach and in bundle sheath cells of P. miliaceum and P. texanum and moderate inhibition in the mesophyll cells of all C4 plants. In the bundle sheath cells of D. sanguinalis the low rate of 14CO2 fixation was stimulated to some extent by the addition of malate and ribose 5-phosphate. The I50 value was 6 × 10?7M for the sensitive cells. Monuron inhibited the oxygen evolution of all seven cell types and their I50 values varied between 3 × 10?7 to 6 × 10?7M. The differential response of isolated plant cells from different species to light-dependent CO2 fixation in the presence of monuron may also be involved in urea herbicide selectivity and undoubtedly is due to the differential photosynthetic pathways present nn them.  相似文献   

8.
The diphenyl ethers acifluorfen (sodium-5-[2-chloro-4-(trifluoromethyl)-phenoxyl]-2-nitrobenzoate), acifluorfen-methyl (methyl-5-[2-chloro-4-(trifluoromethyl)-phenoxyl]-2-nitrobenzoate), and oxyfluorfen (2-chloro-1-[3-ethoxy-4-nitrophenoxy]-4-(trifluoromethyl)benzene have an absolute light requirement for herbicidal activity. CO2-dependent O2 evolution was inhibited in leaf disks obtained from 5-week-old spinach plants as a result of incubation in the light in the presence of each of the three diphenyl ethers. I50's were determined for inhibition by the diphenyl ethers of CO2-dependent O2 evolution in intact chloroplasts obtained from three species of varying susceptibilities (spinach, coffeeweed, and pea). Rankings obtained correlated well with relative susceptibilities and with relative effectiveness of the three compounds tested. Coupled and uncoupled photosynthetic electron transport in susceptible species were unaffected by the three compounds at concentrations in the I50 range. Exposure to herbicidally inactive isomeric analogs of oxyfluorfen and acifluorfen did not affect photosynthesis in leaf disks but was effective in inhibiting photosynthesis in isolated chloroplasts. Photosynthetic abilities of intact tissue were not affected by herbicide treatment in red light. Red light was, however, as effective as white light in mediating the inhibition of photosynthesis in isolated intact chloroplasts by diphenyl ethers. The existence of two photoreceptors for diphenyl ether action, one located at the chloroplast envelope and a second outside of the chloroplast, is suggested as a possible basis for these findings.  相似文献   

9.
The effect of okra yellow vein mosaic virus infection on PS II efficiency and thylakoid membrane protein changes in field-grown bhendi (Abelmoschus esculentus) leaves was studied. The degree of virus infection was determined by means of the ratio of variable to maximum chlorophyll fluorescence (Fv/Fm). Changes in photosynthetic pigments, soluble proteins, nitrate reductase, photosynthetic activities and thylakoid membrane proteins were investigated. Okra yellow vein mosaic virus infection resulted in the reduction of photosynthetic pigments, soluble proteins, ribulose-1, 5-bisphosphate carboxylase activity and nitrate reductase activity. Virus infection caused marked inhibition of PS II activity. The artificial electron donors, diphenyl carbazide and hydroxylamine significantly restored the loss of PS II activity in infected leaves. The marked loss of PS II activity in infected leaves may be due to the loss of 47, 33, 28–25, 17 and 15 kDa polypeptides. It is concluded that the yellow vein mosaic virus infection inactivates the donor side of PS II.  相似文献   

10.
BACKGROUND: Changes in chlorophyll fluorescence have often been advocated as a sensitive biomarker of plant stress, assuming that any kind of plant stress serious enough to affect plant growth will also affect photosynthesis. Glyphosate affects photosynthetic electron transport indirectly by inhibiting sink processes. The question is how fast this inhibition can be observed on CO2 assimilation and ultimately on chlorophyll fluorescence? RESULTS: Experiments measuring CO2 assimilation, conductance and chlorophyll fluorescence using four Kautsky curve parameters on barley (Hordeum vulgare L.) exposed to increasing doses of glyphosate showed a total cessation of CO2 fixation and conductance without significant changes in chlorophyll fluorescence. The decrease in CO2 fixation and conductance was significant 1 day after spraying and corresponded well to the decrease in biomass 5–7 days after spraying. CONCLUSION: A total cessation of CO2 assimilation can take place without affecting chlorophyll fluorescence. Hypotheses concerning what happens to the energy from the photosynthetic apparatus that is not used for CO2 assimilation are discussed. The results question the use of chlorophyll fluorescence as a universal indicator of stress on photosynthetic processes. Also, they demonstrate that changes in gas‐exchange parameters are more sensitive biomarkers for glyphosate toxicity compared with chlorophyll fluorescence. Copyright © 2010 Society of Chemical Industry  相似文献   

11.
UV-B (0.4 W m−2) irradiation and dimethoate (100 and 200 ppm) treatments, singly and in combination, declined the growth, photosynthetic pigment contents and photosynthesis (O2 evolution and CO2-fixation) of cowpea (Vigna unguiculata). Contrary to this, low concentration of dimethoate (50 ppm) caused stimulation on these parameters, while together with UV-B it showed inhibitory effects. Carotenoids (Car) showed varied responses. It was found that carbon-fixation (14CO2) was more sensitive to both the stresses than photosynthetic oxygen evolution. Photosynthetic electron transport activity was reduced by both the stresses, however, 50 ppm dimethoate besides inhibiting photosystem II (PSII) and whole chain activity, showed slight stimulation in photosystem I (PSI) activity. The individual effect of two stresses on PSII activity was probably due to interruption of electron flow at oxidation side of PSII which extended to its reaction center following simultaneous exposure. A similar trend was also noticed in case of CO2 liberation (measured as 14CO2 release) in light and dark. Results suggest that dimethoate (100 and 200 ppm) and UV-B alone caused heavy damage on pigments and photosynthetic activity of cowpea, leading to the significant inhibition in growth. Further, the interactive effects of both the stresses got intensified. However, low concentration (50 ppm) of dimethoate showed stimulation, but in combination, it slightly recovered from the damaging effect, caused by UV-B.  相似文献   

12.
We have studied the inhibitory effect of the herbicides phenmediphan, chloroxuron, dinoseb, dichlobenil, dicamba, 2,4-D, 2,4-DB, and 2,4-DP on photosynthetic CO2 fixation and on the level of intermediates of the CO2 assimilation cycle by isolated chloroplasts, as well as their in vitro activities on the enzymatic systems ribulose-1,5-diphosphate carboxylase and fructose-1,6-bisphosphatase. Phenmedipham showed the strongest inhibition of CO2 assimilation, with an I50 of 0.05 μM, followed by chloroxuron and dinoseb, with a 50% inhibition in the range of 0.5–1 μM. A weaker inhibitory effect, with an I50 of 50 μM, is promoted by 2,4-DB, whereas dicamba and 2,4-DP showed this inhibition at 100 μM; dichlobenil and 2,4-D were completely ineffective. In the presence of phenmedipham and chloroxuron, the trioses-PP-glycerate ratio showed a sharp decrease, which means an inhibition of the P-glycerate reduction step by a low NADPH synthesis; a low ratio is also promoted by 2,4-D, but it may be a consequence of induced collateral metabolic pathways of P-glycerate. Dinoseb showed a 25% inhibition of ribulose-1,5-diphosphate carboxylase activity in the concentration range of 10–100 μM and an I50 of 50 μM of the fructose-1,6-bisphosphatase. Thus these effects could contribute, in addition to the photochemical ones, to an explanation of the dinoseb inhibition of CO2 assimilation by isolated chloroplasts. The other herbicides tested showed a weak or no effect on these enzyme systems.  相似文献   

13.
Timely detection of herbicide resistance at an early stage of crop cultivation is essential to help farmers find alternative solutions to manage herbicide resistance in their fields. In this study, maximum quantum yield of PS II [Fv/Fm = (Fm – Fo)/Fm] was measured at the 4–5 leaf stage to discriminate between herbicide‐resistant and susceptible biotypes of Echinochloa species. The differences in Fv/Fm between herbicide‐resistant and susceptible Echinochloa spp. were consistent with the whole‐plant assay based on I50 (herbicide doses causing a 50% inhibition of Fv/Fm) and GR50 (herbicide doses causing a 50% reduction in plant fresh weight) values and R/S ratios (herbicide resistance index), regardless of the mode of action of the tested herbicides. A PS II inhibitor caused the fastest inhibition of Fv/Fm, compared with ACCase and ALS inhibitors, after herbicide treatment. The required time for discrimination between herbicide‐resistant and susceptible Echinochloa spp. was 64 h after PS II inhibitor treatment, much shorter than those of ACCase and ALS inhibitor‐treated plants, which required 168 and 192 h respectively. The leaf chlorophyll fluorescence assay provided reliable diagnostics of herbicide resistance in Echinochloa spp. with significant time savings and convenient measurement in field conditions compared with the conventional whole‐plant assay.  相似文献   

14.
In this work we have studied the influence of phytoplasma-induced grapevine yellows (yellowing) on some features of the thylakoids from field grown grapevine (Vitis vinifera L. cv. Chardonnay) leaves. Changes in photosynthetic pigments, soluble proteins, ribulose-1,5-bisphosphate carboxylase, photosynthetic activities and thylakoid membrane proteins were investigated. The level of total chlorophyll and carotenoids, on a unit fresh weight basis, showed a progressive decrease in phytoplasma infected leaves. Similar results were also observed for total soluble proteins and ribulose-1,5-bisphosphate carboxylase activity. When various photosynthetic activities were followed in isolated thylakoids, phytoplasma infection caused marked inhibition of whole chain and photosystem (PS) II activity. Smaller inhibition of PSI activity was observed even in severely infected leaves. The artificial exogenous electron donors, DPC and NH2OH significantly restored the PSII activity in both mild and severely infected leaves. The same results were obtained when Fv/ Fm was evaluated by Chl fluorescence measurements. The marked loss of PS II activity in infected leaves was evidently due to the loss of 33, 28–25, 23, 17 and 10 kDa polypeptides. This conclusion was confirmed by immunological studies showing that the content of the 33 kDa protein of the water-splitting complex was diminished significantly in infected leaves. Phytoplasma infection induced a fast degradation of LHCP II which became visible as yellowish colour in leaves.  相似文献   

15.
This study examines the CO2‐mediated influence of plant resistance on crown rot dynamics under continuous cropping of partially resistant wheat line 249 and the susceptible cultivar Tamaroi. Disease incidence, severity, deoxynivalenol and Fusarium biomass were assessed after each cycle in microcosms established at ambient and 700 mg kg?1 CO2 using soil and stubble of these wheat lines from a field experiment with free to air CO2 enrichment. Monoconidial isolates from wheat stubble were collected initially, and after five cropping cycles, to compare the frequency and aggressiveness of Fusarium species in the two populations. Aggressiveness was measured using a high‐throughput seedling bioassay. At elevated CO2, the higher initial incidence in Tamaroi increased with cropping cycles, but incidence in 249 remained unchanged. Incidence at ambient CO2 did not change for either line. Elevated CO2 induced partial resistance in Tamaroi, but not in 249. Increased Fusarium biomass in wheat tissue at elevated CO2 matched raised deoxynivalenol of the stem base in both lines. After five cycles of continuous wheat cropping, aggressiveness increased in pathogenic F. culmorum and F. pseudograminearum by 110%, but decreased in weakly pathogenic F. equiseti and F. oxysporum by 50%. CO2 and host resistance interactively influenced species frequency, and the highly aggressive F. pseudograminearum became dominant on Tamaroi irrespective of CO2 concentration, while its frequency declined on 249. This study shows that induced resistance at elevated CO2 will not reduce crown rot severity, or impede the selection and enrichment of Fusarium populations with increased aggressiveness.  相似文献   

16.
为探讨不同耕作措施下旱作春小麦农田CO2排放以及气候条件、土壤性质和作物管理措施对CO2排放影响的敏感性差异,本文基于DNDC模型,结合定西试验区旱作春小麦农田定点连续监测的CO2排放通量等数据,检验该模型模拟不同耕作措施下CO2排放的适宜性,并对其敏感性进行研究.结果表明:DNDC模型对不同处理下CO2排放的模拟结果...  相似文献   

17.
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18.
The impacts of elevated CO2 and soil water on the population dynamics, adult fecundity and nymphal period of the bird cherry-oat aphidRhopalsiphum padi (Linnaeus) were evaluated in three experiments: (i)Combined effects of CO 2 and soil water on aphid populations. Spring wheat was grown in pots at three CO2 concentrations (350, 550 and 700 ppm) and three soil water levels (40%, 60% and 80% of field water capacity, FWC) in field open-top chambers (OTC) and infested with the bird cherry-oat aphid. Aphid population dynamics were recorded throughout the growing season; at the same time, adult fecundity and duration of the nymphal period were recorded. Chemical composition of spring wheat leaves was also analyzed. (ii)Indirect effects of CO 2 concentrations and soil water on aphid adult fecundity and nymphal period. The experiment was conducted with the leaf discs method in the laboratory. Aphids were reared on leaf discs excised from the treated wheat in OTC with different CO2 and soil water levels. (iii)Direct effects of CO 2 concentrations on aphid adult fecundity and nymphal period. Aphids were reared on leaf discs excised from the wheat grown under natural conditions. The experiment was conducted with the leaf disc method in OTC with the three CO2 concentrations. It was found that the direct effect of CO2 concentration on aphid population parameters was minor. CO2 and soil water affected aphid population indirectly through their effects on wheat characteristics. The aphid population under 550 ppm CO2 was far larger than the one under 350 ppm CO2, whereas the population under 700 ppm CO2 was slightly higher than that under 550 ppm CO2. The largest aphid population was obtained with the 60% soil water treatment, regardless of CO2 treatment. The effects of CO2 concentration on aphid population were, however, not significantly correlated with soil water level. Adult fecundity increased with CO2 concentration, the highest fecundity being achieved under 60% FWC treatments. The nymphal period was not affected by CO2 concentration. The shortest period occurred under 60% FWC. Atmospheric CO2 and soil water had significant effects on the chemical composition of the wheat leaves. Aphid population size was positively correlated with leaf water content, concentrations of soluble proteins, soluble carbohydrates and starch, and negatively correlated with DIMBOA and tannins concentrations. http://www.phytoparasitica.org posting Oct. 20, 2003.  相似文献   

19.
The effect of DDT (2,2-bis-(p-chlorophenyl)-1,1,1-trichloroethane) on carbon assimilation of a green alga, Selanastrum capricormutum was studied. DDT at concentrations between 3.6 and 36 ppb was inhibitory to the photosynthetic CO2 fixation (ethanol-soluble and/or ethanol-insoluble) and the longer the exposure to DDT, the greater the inhibition. Kinetic studies of photosynthetic CO2 fixation indicated that DDT stimulated the incorporation of carbon-14 into glycolic acid, a major compound of photorespiration and caused the concomittant suppression of flow of carbon-14 into aspartic acid, a major component of the C4-dicarboxylic acid pathway. The shift from an efficient pathway into a nonefficient pathway by DDT was interpreted to be through interruption of cyclic photophosphorylation.  相似文献   

20.
The objective was to study CO2 fixation and photoassimilate partition in coffee (Coffea arabica) seedlings infested with the lesion nematode Pratylenchus coffeae. Seedlings infested with 0, 1000 and 8000 Pratylenchus coffeae nematodes were exposed to 14CO2 and the incorporation and distribution of radioactivity were followed in the roots, stems and leaves. Fresh mass, pigments, soluble sugars, sucrose and specific radioactivity of sucrose in the plant parts were determined. At the highest level of infestation almost all the parameters were significantly changed showing the carbon fixation in the leaves and partitioning to the roots were decreased. Since lesion nematodes are not sedentary and do not form feeding sites that could be characterised as metabolic sinks, it is suggested that their damage is more readily expressed by the leaves, through a reduction in photosynthesis and phloem transport.  相似文献   

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