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利用人用诊断试纸对2只绿狒两个月经周期内尿中的促黄体生成素（LH）的变化情况进行测定。测算出绿狒的卵泡形成、排卵和黄体生成的时间，结合对绿狒繁殖行为的观察，掌握了其发情周期及排卵规律，为提高配种受胎率提供了科学依据。 相似文献
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《养殖与饲料．饲料世界》2020,(9)
本试验从15只处于发情前期母犬中随机选取6只母犬,检测其促黄体生成素(LH)、促卵泡素(FSH)和孕酮(P4)3种激素,实现对母犬发情期连续的监测过程。采用静脉采血,抗凝低温离心后分别测定促黄体生成素(LH)、促卵泡素(FSH)、孕酮(P4)的含量,促黄体生成素采用人促黄体生成激素放射免疫分析药盒的方法测定,促卵泡素采用人促滤泡激素放射免疫分析药盒的方法测定,孕酮采用孕酮放射免疫分析药盒的方法测定。测定结果显示:母犬发情期的促黄体生成素的变化范围为5.16～6.82 ng/mL(除峰值外),其峰值平均值是116.71ng/mL;母犬发情期的促卵泡素的变化范围为3.18～4.78 ng/mL(除峰值外),其峰值平均值是43.56 ng/mL;母犬发情期的孕酮的变化范围为0.199 1～8.506 3 ng/mL,其峰值平均值是8.506 3 ng/mL。母犬在发情时,可出现LH释放波,并在LH开始释放后24 h内或开始发情的1～2 d内排卵。 相似文献
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不同繁殖状态的鸵鸟血浆FSH、LH水平比较分析 总被引：1，自引：1，他引：1
目前对鸟类FSH（促卵泡刺激素）、LH（促黄体生成素）测定的有关报道主要集中在鸡、鸭、鹌鹑和火鸡阳的相关研究，而对鸵鸟生殖激素的研究主要集中在有关激素的分离纯化、结构、生化和免疫学特征的研究上，仅见Allan Degen（1994）测定了雌雄鸵鸟的几种生殖激素年周期的变化。有关鸵鸟在不同繁殖状态下排卵周期内血浆FSH、LH水平变化尚未见报道。本研究采用人（FSH、LH）-RIA系统分别测定鸵鸟血浆中的激素水平，探讨这种方法的可行性，并通过该法获得不同生理状态下成年母鸵鸟的血浆FSH、LH水平变化。 相似文献
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试验旨在研究热应激对金定鸭生产性能、血液生殖激素、卵巢生殖相关基因表达量及免疫指标的影响。试验选用产蛋期400日龄的金定鸭,设置对照组（常温组）、试验组（热应激）。对照组环境温度（25±2）℃,试验组环境温度（35±2）℃;测定热应激下金定鸭生产指标、血清生殖激素指标和免疫指标、卵巢生殖基因表达量。结果显示：与对照组相比,试验组产蛋率、总蛋重和平均蛋重极显著降低（P<0.01）;血清促卵泡生成素（FSH）含量显著降低（P<0.05）;卵巢促性腺素释放激素（GnRH）基因表达量显著降低（P<0.05）,促黄体生成素（LH）基因表达量极显著降低（P<0.05）;血清IgG含量极显著降低（P<0.01）,肿瘤坏死因子-α(TNF-α)含量显著升高（P<0.05）。由此可见,热应激对金定鸭生产性能、生殖激素水平及免疫指标产生不利影响,其中产蛋率、总蛋重、平均蛋重、血清FSH及卵巢LH、GnRH基因表达量均显著降低,蛋鸭炎性因子水平显著增加,机体免疫力显著下降。 相似文献
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利用产蛋鸡小黄卵泡膜细胞培养模型研究黄体生成素（LH）对等级前发育的小黄卵泡膜细胞增殖的影响以及LH作用的信号转导途径。单层培养的外层膜细胞用LH（0.1～100μg/L）、蛋白激酶A（PKA）激活剂forskolin（FRSK,0.1～10μmol/L）及抑制剂H89（0.01～1μmol/L）或蛋白激酶C（PKC）激活剂佛波酯（PMA,0.1～10nmol/L）及抑制剂H7（0.01～1μmol/L）单独或共同处理,36 h后测定膜细胞增殖的变化。结果显示,LH和FRSK促进小黄卵泡膜细胞的增殖,且LH的促增殖作用可被H89阻断,而PMA无显著的促进作用,且H7不能阻断LH的促增殖作用。由此推断LH的促小黄卵泡外层膜细胞增殖作用主要是通过PKA途径进行的。 相似文献
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李菊译者 雷蕾校者 Seaman C. Moritz J. Falkenstein E. Van Dyke K. Casotti G. Klandorf H. 《饲料与畜牧》2008,(12):63-63
此试验目的是为了准确定义限饲条件下肉用种母鸡的光诱导促黄体激素（LH）分泌的反应曲线,并与光照反应曲线进行比较,以改进母鸡初产年龄（AFE）。选择平均体重为2．0kg的20周龄肉鸡,分别转移经受8、9、9．5、10、10．5、11、11．5、12、12．5、13、14、18h的光照,4d后测定血浆促黄体激素变化浓度,并记录个体的初产年龄。促黄体激素分泌最先显著增加的是转移到11．5h光照组的鸡, 相似文献
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<正> 家兔是诱发排卵的动物,发情母兔经交配刺激后,脑下垂体前叶释放促黄体生成素(LH),从而引起卵巢排卵。因此,在家兔人工受精前用激素促使母兔卵泡发育和排卵,就可以不经公兔爬跨而使母兔受胎。 我们应用国产孕马血清促性腺激素(PMSG)和促卵泡刺激素(FSH),作为促使卵泡发育和发情的药物;用绒毛膜促性腺激素(HCG)和促黄体生成素(LH),作为促使卵巢排卵的药物,诱导家兔同期发情和提高受胎率。 相似文献
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将30只公鸡随机分为试验组和对照组,每组15只,对照组饲喂基础日粮(每日两次)+自来水,试验组饲喂基础日粮(每日两次)+3.25g/l的NaF水,80d后,检测每只公鸡血浆促黄体生成素的水平。以此探讨慢性氟中毒对公鸡促黄体生成素水平的影响,结果试验组血浆促黄体生成素平均含量为(0.178±0.026)U/l,两组t检验比较差异极显著(P<0.01)。说明氟严重降低公鸡的促黄体生成素水平。 相似文献
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为了检测山羊迷走神经结状神经节中是否有促黄体生成素受体(LHR)的存在,探讨促黄体生成素(LH)是否能够影响结状神经节内脏感觉神经元的功能活动。本试验采用免疫组织化学SP法观察LHR在结状神经节中的分布特征,利用IPP 6.0图像半定量分析系统分析LHR在结状神经节中阳性神经元和阳性非神经元上的表达量差异。结果显示,LHR主要分布在神经元细胞膜和细胞质中,呈棕黄色为强阳性。在带核的神经元中,神经元细胞核呈圆形空泡状,LHR为阴性反应。神经元群间的神经纤维呈淡黄色,LHR为弱阳性。LHR在神经元胞体上的相对表达量极显著高于节内其他阳性非神经元结构(P0.01)。结果表明,山羊结状神经节内脏感觉神经元是LH作用的主要靶细胞,提示LH有可能通过作用于结状神经节内脏感觉神经元胞体上的LHR参与调节内脏器官感觉信息的传导。 相似文献
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Trisomboon H Malaivijitnond S Cherdshewasart W Watanabe G Taya K 《The Journal of reproduction and development》2007,53(2):395-403
This study investigated the changes in the urinary hormone levels of female monkeys (Macaca fascicularis) after single-dose and long-term treatments with Pueraria mirifica (PM). The monkeys were separated into 3 groups (n=3) and orally treated with 10, 100, or 1,000 mg of PM in each group. Two series of experiments were performed. In the first series of experiments, the monkeys were orally treated with a single dose of PM. The experimental schedule was divided into a one menstrual cycle pretreatment period and a two menstrual cycle post-treatment period. In the second series of experiments, the monkeys were orally treated daily with PM for 90 days. The experiment schedule was divided into a one menstrual cycle pretreatment period, a three menstrual cycle treatment period, and a two menstrual cycle post-treatment period. Urinary samples were collected daily and assayed for the FSH, LH, estradiol, and progesterone levels. The results showed that there were no changes in the FSH, LH, estradiol, and progesterone levels after treatment with a single dose of 10, 100, or 1,000 mg of PM or after daily treatment with 10 mg of PM for 90 days compared with the levels observed during the pretreatment period. Daily treatment with 100 mg and 1,000 mg of PM for 90 days only produced a clear reduction in the urinary FSH levels. This suggests that changes of urinary FSH levels can be considered an indicator for study of estrogenic effects on hormonal levels in female monkeys. 相似文献
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The aim of this study was to investigate the potential role of foetal androgens in determining the sexual dimorphism in LH gene expression. Starting on day 30 p.c. pregnant sows were treated i.m. with testosterone propionate (TP) three times at 2-day intervals (TP30 treatment) or received additional TP treatment starting on day 40 p.c. (TP30/40). Sows were allowed to farrow and after frequent blood samples for LH determination were collected prepubertally (6 months) from the female offspring anterior pituitary LHbeta subunit mRNA levels were determined. In Experiment 2 pregnant sows were treated as TP30 before or received similar treatment starting on day 40 p.c. (TP40), but anterior pituitary LHbeta mRNA and plasma LH concentrations were determined at day 80 p.c. TP30 or TP30/40 treatment did not affect mean plasma LH concentrations nor LHbeta mRNA levels at 6 months of age but caused marked masculinization of external genitalia. At day 80 p.c. LHbeta mRNA and plasma LH levels were higher in female than in male foetuses. TP40 treatment suppressed LHbeta mRNA and plasma LH levels while TP30 treatment had no effect on LHbeta mRNA levels but caused masculinization of external genitalia in contrast to TP40. Our findings support the notion that the peak in plasma testosterone observed by others in the male pig foetus 5 weeks p.c. not only determines sexual differentiation of the LH surge mechanism but also LH gene expression in the foetus. The critical period for this process seems to succeed phenotypic differentiation (which appears to be largely completed before day 40 p.c.). The tonic mode of prepubertal LH gene expression and LH secretion in female pigs is not affected greatly by testosterone treatment at the stages of development that were investigated. 相似文献
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目的：探讨实验猕猴生殖道电阻值在月经周期里不同时期的变化规律，结合E2分泌水平，为预测排卵时间提供基础科学数据，满足科研实验需求。方法：利用动物排卵测定仪检测实验猕猴阴道子宫后穹隆处的电阻值，结合检测血清E2分泌水平来说明阴道电阻值与E2分泌水平的关系，预测排卵时间。结果：在猕猴卵泡期初始，月经周期第4 d的阴道电阻值由587±184Ω逐步下降至排卵期第11 d的213±179Ω，随着黄体期的到来，电阻值又急速上升，到第16 d上升至365±148Ω，第22 d平稳在390±140Ω水平。血清E2高峰日出现在月经周期的第10 d，浓度为187.8±123.3 pg/mL。结论：生殖道电阻值随着E2变化而呈现出有规律性的变化，它是一种简便易行、无创伤、无副作用监测预报排卵的方法。 相似文献
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Kristina Nordéus Renée B?ge Hans Gustafsson Patrice Humblot Lennart S?derquist 《Acta veterinaria Scandinavica》2012,54(1):26
Background
Declining fertility is a major concern for dairy farmers today. One explanation is shorter and weaker expression of oestrus in dairy cows making it difficult to determine optimal time for artificial insemination (AI). Chemical communication is of interest in the search for tools to detect oestrus or to synchronise or enhance oestrous periods. Pheromones, used in chemical communication within species, can influence reproduction in different ways. The aim here was to investigate whether oestrous cycle length, and duration and intensity of oestrous expression in dairy heifers could be manipulated through exposure to pheromones in oestrual substances from other females.Methods
Beginning on day 16 of two consecutive control oestrous cycles, ten heifers of the Swedish Red Breed (SRB) were exposed to water. During the two following cycles the heifers were exposed to urine and vaginal mucus, obtained from cows in oestrus. Cyclicity parameters were monitored through hormone measurements, oestrus detection and ultrasonographic examination.Results
We found no difference in cycle length or in duration of standing oestrus between control and treatment. We did, however, find a tendency of interaction between type of exposure (control or treatment) and cycle number within type of exposure for cycle length (p = 0.068), with the length differing less between the treatment cycles. We also found a tendency of effect of type of exposure on maximal concentration (p = 0.073) and sum of concentrations (p = 0.063) of LH during the LH surge, with values being higher for the control cycles. There were also significant differences in when the different signs of oestrus occurred and in the intensity of oestrous expression. The score for oedema and hyperaemia of external genitalia was significantly higher (p = 0.004) for the control cycles and there was also a significant interaction between type of exposure and time period for restlessness (p = 0.011), with maximum score occurring earlier for treatment cycles.Conclusions
No evidence of altered oestrous cycle length or duration of oestrus after exposure of females to oestrous substances from other females was found. Expression of oestrus, and maybe also LH secretion, however, seemed influenced by the exposure, with the effect of treatment being suppressive rather than enhancing. 相似文献15.
T L Martin L V Swanson L H Appell K E Rowe F Stormshak 《Domestic animal endocrinology》1990,7(1):27-34
Two experiments were conducted to investigate the response of the bovine corpus luteum to surges of luteinizing hormone (LH) induced by natural gonadotropin-releasing hormone (GnRH) administered twice during the same estrous cycle. In experiment 1, eight mature beef cows, each cow serving as her own control, were injected intravenously (iv) with saline on days 2 and 8 of the cycle (day of estrus = day 0 of the cycle), then with 100 micrograms GnRH on days 2 and 8 of the subsequent cycle. Jugular blood samples were taken immediately prior to an injection and at 15, 30, 45, 60, 120 and 240 min postinjection, to quantitate changes in serum luteinizing hormone. Blood was also collected on alternate days after an injection until day 16 of the cycle, to characterize changes in serum progesterone concentrations. Although exogenous GnRH caused release of LH on days 2 and 8 of the cycle, the quantity of LH released was greater on day 8 (P less than .025). Serum levels of progesterone after treatment with GnRH on day 8 of the cycle did not differ significantly from those observed during the control cycles of the heifers. Because exposure of the bovine corpus luteum to excess LH, induced by GnRH early during the estrous cycle, causes attenuated progesterone secretion during the same cycle, these data suggest that a second surge of endogenous LH may ameliorate the suppressive effect of the initial release of LH on luteal function. Duration of the estrous cycle was not altered by treatment (control, 20.4 +/- .5 vs. treated, 20.4 +/- .4 days).(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
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Dees WL Dearth RK Hooper RN Brinsko SP Romano JE Rahe H Yu WH McCann SM 《Domestic animal endocrinology》2001,20(4):3791-288
Recent studies have shown that lamprey gonadotropin-releasing hormone (l-GnRH) is localized in the mammalian brain, and that l-GnRH-III, can selectively induce FSH secretion in the rat both in vivo and in vitro. Consequently, the purpose of this study was to determine if l-GnRH-III could elicit selective FSH release in cattle and compare this response with that to mammalian luteinizing hormone releasing hormone (m-LHRH). Cattle were chosen as the animal model because previous studies have demonstrated that FSH and LH are secreted by separate gonadotropes in that species. For these studies, crossbred cycling heifers were implanted with jugular cannulae and l-GnRH-III was infused either between Days 9–14 or on Day 20 of the estrous cycle. Blood samples were collected both before and following peptide infusion. Our results demonstrate that during Days 9–14 of the estrous cycle (luteal phase), when progesterone levels averaged between 4 and 5 ng/ml, a dose of 0.25 mg of l-GnRH-III induced the release of FSH (P < 0.05), but not LH. A 0.5 mg dose of l-GnRH-III caused a greater release of FSH (P < 0.01), but still did not induce LH release. Higher doses of the peptide were capable of significantly releasing both gonadotropins. Importantly, during the luteal phase, doses of 0.5 and 2 mg of m-LHRH were ineffective in stimulating FSH, but did elicit marked increases (P < 0.001) in LH. Again, progesterone levels averaged 4–5 pg/ml. In order to assess gonadotropin releasing ability of l-GnRH-III at a different phase of the estrous cycle, some animals were administered the peptide on Day 20, when progesterone levels were below 1.0 pg/ml. At this time, the l-GnRH-III induced the release of LH (P < 0.01), but not FSH. Overall, our results demonstrate that l-GnRH-III can selectively induce FSH in cattle during the luteal phase, whereas m-LHRH was ineffective in that regard. Furthermore, the fact that l-GnRH-III can selectively stimulate FSH when serum progesterone is high, and LH when serum progesterone is low, suggests its actions are under strong control of this steroid. We suggest the FSH releasing capacity of l-GnRH-III in cattle could render this peptide useful for enhancement of reproductive efficiency in this species. 相似文献
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P Concannon S Whaley D Lein R Wissler 《American journal of veterinary research》1983,44(10):1819-1821
Variation in canine gestation length was studied in a Beagle colony (n = 290) in which apparent gestation length, estimated as the interval from the day of first mating to the day of parturition, ranged from 57 to 72 days, and averaged 65.3 +/- 0.2 days. The interval from the day of the peak in luteinizing hormone (LH) to parturition was less variable and ranged from 64 to 66 days and averaged 65.1 +/- 0.1 days (n = 54). Apparent gestation lengths less than or equal to 61 days all resulted from mating greater than or equal to 3 days after the LH peak; those greater than or equal to 68 days all followed initial or single matings occurring greater than or equal to 2 days before the LH peak. Fertile single matings 3 days before the LH peak provided evidence that the potential postcoital fertile longevity of canine sperm is at least 6 days and thus contributed, along with variability in the onset of estrus, to the observed variation in apparent gestation length in the dog. The limited range in the interval from the day of the preovulatory LH peak to the day of parturition (64, 65, or 66 days) demonstrates a considerable regularity in the sequential events of gestation in the dog. 相似文献
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为在体外成纤维细胞培养体系上探讨盐酸川芎嗪抗鸡传染性法氏囊病病毒的活性及其作用机制,采用MTT法结合细胞病变观察法,通过病毒感染抑制、复制抑制、吸附抑制和直接灭活试验进行评价。结果显示,盐酸川芎嗪在体外对鸡传染性法氏囊病病毒具有较强的作用,其EC50为（92．52±21．13）mg／L,SI≥21．62,最大抑制率达83．7％;盐酸川芎嗪对病毒具有较强的直接杀灭作用,但不能阻止病毒吸附;在复制抑制试验中,盐酸川芎嗪的抗病毒作用具有时间依赖性,分别在病毒吸附O、2、4h时将盐酸川芎嗪加入到体系中,盐酸川芎嗪显示出较强的抗病毒作用,随着病毒吸附时间的延长,盐酸川芎嗪对鸡传染性法氏囊病病毒的抑制作用逐渐减弱。结果表明,盐酸川芎嗪可通过直接杀灭鸡传染性法氏囊病病毒或／和干扰其早期复制过程而表现出抗病毒活性。 相似文献