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1.
Summary Callus cultures were initiated from inflorescence explants of wheat, rye and triticale on MS medium supplemented with 2 mgl-1 2,4-D+5% CW or 2 mgl-1 2,4-D+0.5 mgl-1 BA. On transfer of the cultures to medium supplemented with 15% CW+0.2 mgl-1 NAA or 1 mgl-1 BA+0.1 mgl-1 IAA, shoot buds and embryoids were produced. Full fledged plantlets obtained on MS medium supplemented with NAA were transferred to the field. Cytological analysis showed the plants to be diploid. However, the regenerated plantlets were shorter, produced fewer tillers and had lower fertility compared to the control.Abbreviations BA
Benzyladenine
- CW
coconut water
- IAA
indoleacetic acid
- NAA
-naphthaleneacetic acid
- 2,4-D
2,4-dichlorophenoxyacetic acid 相似文献
2.
大叶黄杨幼茎愈伤组织诱导的研究初报 总被引：2，自引：0，他引：2
以大叶黄杨的幼茎段为外植体,在添加BA和NAA、IBA、2,4-D不同激素组合的MS培养基上培养,对其愈伤组织进行诱导试验。结果表明：①生长素对愈伤组织诱导的效应为2,4-D >NAA>IBA;②在不同激素组合培养基上均可诱导出愈伤组织,其中MS+BA1.0~2.0 mg/L+NAA 1.0~1.5 mg/L、MS+BA1.5~2.0 mg/L+IBA1.0~1.5 mg/L、MS+BA0.5~1.0 mg/L+2,4-D 0.5~1.5 mg/L等对愈伤组织的诱导效果最好,其诱导率分别为74.3%、65.3%、81.1%。因此,通过科学配制不同激素组合的MS培养基,就能有效地诱导出大叶黄杨幼茎的愈伤组织。 相似文献
3.
Plant regeneration was achieved from immature embryo-derived, calli of Pisum sativum. Embryo axes were separated from cotyledons and cultured on different media containing BAP and NAA until plantlet regeneration. Rooting of the plantlets was obtained on MS medium supplemented with 2 mg/1 IBA. Frequency of regeneration was shown to be under the influence of the genotype. Histological preparations showed de novo origin of the shoots via organogenesis. Out of 2C regenerated plantlets, 11 were diploids (2n = 14) arid 9 aneusomatic (chromosomal mosaics) with chromosome numbers ranging from 12 to 16. 相似文献
4.
Summary Callus growth and its subsequent regeneration into complete plantlets was achieved from in vitro cultured anthers ofBrassica nigra (L.)Koch. Callus was induced on a modified N6 medium containing trace elements, organics of B5 medium and 2 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D). Morphogenesis of callus in the form of shoots on MS medium containing indole-3-acetic acid (IAA) and N6-benzyladenine (BA) 0.5 mg/l each and embryoids on MS medium containing 0.5–1.0 mg/l IAA and 3.0–5.0 mg/l BA could be accomplished. Chromosomal analysis revealed presence of 41% haploids (n=8) amongst the regenerated plants. 相似文献
5.
银木的组织培养及植株再生技术研究 总被引：1，自引：1，他引：0
以银木2年生小苗的茎段为外植体,建立银木的组织培养和再生体系。研究不同种类、不同浓度生长调节剂、光照条件等因素对银木组培各阶段产生的影响。结果表明：适合嫩茎段腋芽初诱导与生长对培养基的选择不甚严格,在基本培养基MS中即可诱导;增殖培养基为MS+ BA 0.5 mg/L+ NAA 0~ 0.05 mg/L,增殖系数可达3.5以上;生根的最佳培养基为1/2MS+ NAA 0.1 mg/L + IBA 0.2 mg/L,生根率达98%以上。银木的高效快繁体系建立为银木的开发利用和工厂化育苗提供可靠技术依据。 相似文献
6.
Intergeneric hybrid plants between Colchicaceous ornamental plants, Sandersonia aurantiaca and Gloriosa rothschildiana, have successfully been produced via ovule culture. After 5 days of reciprocal cross-pollination, a few pollen tubes were observed in the ovary. Although seeds were obtained in both reciprocal cross-combinations, they did not germinate under ex vitro conditions. Ovules with placental tissues isolated 14 days after cross-pollination of S. aurantiaca × G. rothschildiana were cultured on a medium containing 0.01 mg l–1 each of -naphthaleneacetic acid (NAA) and 6-benzyladenine (BA), on which 41.5% of ovules swollen and produced callus-like structures within 10 weeks. When such swollen ovules were transferred to a medium containing 0.1 mg l–1 each of NAA and BA, 7.5% of the initially cultured ovules produced rhizome-like structures within 6 weeks. Among the rhizome-like structures, those derived from two independent ovules (3.7% of the initially cultured ovules) produced multiple shoots following transfer to a medium containing 0.25 mg l–1 NAA and 2.5 mg l–1 BA. Multiple shoot-derived plantlets were established on a plant growth regulator-free medium, and they were successfully transplanted to pots. Early verification of their hybridity was accomplished by flow cytometry (FCM) analysis, chromosome observation and rDNA analysis. 相似文献
7.
Summary
Cajanus platycarpus a wild species of pigeonpea incompatible with the cultigen has many desirable characters important for the improvement of cultivated pigeonpea. In the present study, barriers to hybridization were studied and were identified as post-zygotic. Efficient embryo rescue techniques were developed. As a result, hybrids were produced. Morphological and cytological studies as well as esterase isozyme band pattern confirmed the hybrid nature of the plants. The F1 hybrids were completely pollen sterile. Meiotic studies were carried out to check for the cause of pollen sterility.Abbreviations MS
Murashige & Skoog's (1962) medium
- BAP
6-Benzylaminopurine
- IBA
Indole-3-butyric Acid
- NAA
Naphthaleneacetic Acid 相似文献
8.
Using three varieties of Brassica rapa, cv. Hauarad (accession 708), cv. Maoshan-3 (714) and cv. Youbai (715), as the maternal plants and one variety of B. oleracea cv. Jingfeng-1 (6012) as the paternal plant, crosses were made to produce interspecific hybrids through ovary culture techniques. A better response of seed formation was observed when ovaries were cultured in vitro at 9–12 days after pollination on the basal MS and B5 media supplemented with 6-benzylaminopurine (BA) and naphthylacetic acid (NAA). The best response was observed for cross 714×6012 with the rate of seeds per ovary reaching 43.0%. Seeds for cross 715×6012 showed the best germination response (66.7%) on the regeneration medium (MS+1.0 mg l–1 BA+0.05 mg l–1 NAA). In all three cross combinations, good response in terms of root number and length of plants was observed on the root induction medium (MS+1.0 mg l–1 BA+0.1 mg l–1 NAA). A better response was observed for the regenerated plants cultured for 14 days than for 7 days. The ovary-derived plants with well-developed root system were hardened for 8 days and their survival rate reached over 80%. Cytological studies showed that the chromosome number of all plants tested was 19 (the sum of both parents), indicating that these regenerated plants were all true hybrids of B. rapa (n = 10) × B. oleracea (n = 9). The regenerated plants were doubled with colchicine treatment, and the best response in the crosses 708×6012, 714×6012 and 715×6012 was observed when treated with 170 mg l–1 colchicine for up to 30 h and their doubling frequency reached 52, 56 and 62%, respectively. 相似文献
9.
蝴蝶兰组织培养体系的建立及其关键技术研究 总被引：1，自引：0，他引：1
以蝴蝶兰为供试材料,采用幼嫩的花梗、开花后的花梗和茎尖作为外植体,探索蝴蝶兰组织培养技术体系,着重研究各阶段的最佳培养基培养及培养程序。结果表明,开花后的花梗是诱导芽的良好材料,适合花梗诱导芽的培养基是MS+BA1mg/L+NAA0.1 mg/L。防止茎尖褐化的方法是将1%VC过滤灭菌后倒入培养皿,在培养皿中切取茎尖,接种到添加了0.2%PVP的培养基上,适宜茎尖诱导芽的培养基是VW+ BA3mg/L+NAA0.5 mg/L+椰汁200ml/L。适合增殖的培养基是MS+ BA1mg/L+NAA1 mg/L。适合生根的培养基是?MS +IBA1mg/L+NAA1 mg/L。生根的组培苗,置于温室进行经过适当的炼苗处理,移栽基质为湿润海藻,环境湿度保持在80%以上。 相似文献
10.
11.
以窄冠白杨为试材,对其组培快繁与再生体系的建立进行了初步的研究。研究表明,抗氧化剂聚乙烯吡咯烷酮（PVP）和抗坏血酸（Vc）能有效降低外植体的褐化;该品种外植体增殖的最适培养基为1/2MS+BA0.3mg/L+NAA0.05mg/L,平均分化率为87%,增殖系数达7.5倍,与其它处理组合均呈极显著差异。硝普钠（SNP）12mg/L极显著地促进叶片不定芽分化,其再生频率与再生芽个数分别达到96.5%与8.2。窄冠白杨适宜的生根培养基为1/2MS+IBA0.3mg/L。 相似文献
12.
春兰‘金荷鼎’ב赤蕙’杂交根状茎芽分化及生根的研究 总被引：1，自引：1，他引：0
以‘金荷鼎’ב赤蕙’根状茎为材料,研究根状茎芽分化和生根情况,包括BA与IBA的浓度配比对分化的影响和BA与NAA的浓度配比对生根的影响。结果表明,1/2MS+ IBA 0.2 mg/L+ BA 2 mg/L为春兰‘金荷鼎’ב赤蕙’根状茎的最佳分化培养基;春兰杂交兰最佳生根培养基为1/2MS+ BA 0.1 mg/L+ NAA 2 mg/L。杂交育种与组织培养技术结合是国兰新品种选育的有效方法。 相似文献
13.
以勿忘我幼嫩花序为外植体,进行组织培养快繁技术的研究。试验结果表明：在起始培养基附加20 mg/L的苯甲酸钠对抑制污染有一定作用。其最适的芽增殖培养基为：MS+BA1.0+NAA0.1+3%砂糖,交替附加0.5 mg/LGA3的效果为好,诱导生根培养基为1/2MS+NAA0.4+2%砂糖为佳。 相似文献
14.
Summary Four different hybridization experiments were carried out to obtain interspecific hybrids with Spanish cultivars of lentil (Lens culinaris M.). In hybridization experiments I and II, undertaken with only pollination and pollination with the addition of gibberellic acid after fertilization, respectively, no lentil hybrids were recovered. A single interspecific hybrid with L. odemensis was obtained in experiment III using the embryo rescue protocol of Cohen et al. (1984), in this case, a crossing efficiency of 0.11% and a rescue efficiency of 2.5% were obtained. Hybridization experiment IV used a specific embryo rescue protocol developed in this study. In this experiment, ovule-embryos of 18 DAP were cultured on MS salts with 1% sucrose and 1 μ M IAA + 0.8 μ M KN; after two weeks, embryos were released from the ovular integuments and cultured on the same medium for another two weeks in upright position. Afterwards, the embryos were transferred to test tubes containing the same medium and one month later plantlets were obtained. Using the above protocol, out of a total of 1707 pollinations, 6 interspecific hybrids with L. odemensis, 2 with L. nigricans and one with L. ervoides were recovered, yielding on average a crossing efficiency of 0.53% and an average rescue efficiency of 8.26%. Taking into consideration only the interspecific crossing blocks in which hybrids were recovered, the crossing efficiency with L. odemensis was 9%, while with both L. nigircans and L. ervoides the crossing efficiencies were 3%. Rescue efficiencies based on hybrids recovered per number of ovules cultured ranged between 50–100%. 相似文献
15.
Summary Week old seedlings of indica rice variety Jaya obtained on basal MS medium and further sub-cultured on agar solidified MS medium supplemented with cytokinins, sucrose (3% w/v) and mannitol (1% w/v) lead to development of multiple shoot buds. Shoot cultures were maintained and multiplied in liquid medium containing BAP 5 mg l-1, sucrose (3% w/v) and mannitol (1% w/v). Profuse rooting was obtained on transfer to MS liquid medium containing IBA 1 mg l-1 and sucrose (3% w/v). Complete plants were successfully transferred to soil and grown to maturity. 相似文献
16.
魔芋块茎组织培养快速繁殖技术的研究 总被引：3，自引：0，他引：3
以魔芋幼芽附近的块茎组织和其它部分的块茎组织为外植体,接种于I/2MS 1.0mg/IBA 0.01mg/INAA的培养基中,从增大的块茎组织表面直接诱导幼芽。增大的块茎组织分割后接种于MS 1.0mg/IBA 0.01mg/INAA的培养基中进行增殖培养,6个月后,块茎组织的增殖率最高可达757倍。诱导出的幼芽转入不含激素的MS培养基中,形成幼苗,幼苗切块转入MS 0.1mg/INAA的生根培养基中,幼苗生根。试管苗移栽6个月后获得子块茎。从而建立起魔芋块茎组织培养快速繁殖技术程序。 相似文献
17.
以‘洛阳红’牡丹组培苗为试材,探讨了不同基本培养基、激素配比、培养条件、抗褐化物质对‘洛阳红’牡丹继代苗增殖生长、生根及褐化的影响。结果表明：DKW基本培养基适宜‘洛阳红’牡丹继代增殖;培养基中附加2.0～3.0mg/L BA与0.5mg/L IAA或0.05mg/L NAA配比有利于继代苗分化、生长,而附加玉米素ZT效果不佳;20～25℃对‘洛阳红’继代苗增殖影响不大,30℃下试管苗很快老化、枯死,不宜采用;1/2MS附加0.2mg/L NAA或2.0mg/L IAA与2.0mg/L IBA配比可促进‘洛阳红’牡丹试管苗不定根诱导;继代和生根培养基中附加0.5g/L PVP可有效减轻‘洛阳红’牡丹组培苗褐变,促进继代苗分化、生长,但对生根没有明显影响。 相似文献
18.
月季茎段组织培养的研究 总被引：6，自引：0，他引：6
以MS为基本培养基，附加不同浓度的细胞分裂素(BA)和生长素(NAA)，对月季茎段组培快繁进行了研究。结果表明：初代培养阶段，以较低浓度的细胞分裂素和生长素配比对侧芽萌发具有较好的促进作用，适宜的培养基为MS BA1.2mg/L NAA0．3mg/L。继代增殖阶段，以培养基MS BA1.5mg/L NAA0.05mg/L效果较好。生根阶段，最适宜的培养基为1／2MS IBA0．5mg/L。 相似文献
19.
Mikihisa Umehara Takayuki Sueyoshi Katsumi Shimomura Masatoshi Iwai Masayoshi Shigyo Keita Hirashima Takao Nakahara 《Euphytica》2006,148(3):295-301
Interspecific hybridization is an effective method to generate a new crop that gains available functions in a short time. Interspecific hybrids (2n= 16) betweenAllium fistulosumL. (2n= 16) andAllium schoenoprasumL. (2n= 16) were produced by reciprocal crossings through ovary culture, but the hybrids were much fewer in the combination usingA. schoenoprasumas a seed plant. All the hybrids have eight long chromosomes originated fromA.fistulosumand eight short chromosomes originated fromA. schoenoprasum. In addition, the hybridity was confirmed by randomly amplified polymorphic DNA (RAPD) analysis and cleaved amplified polymorphic sequence (CAPS) analysis of the rDNA internal transcribed spacer (ITS) region. The interspecific hybrids showed a vigorous growth habit; their foliage was slightly bloomy and deep green. The hybrids did not form bulbs, but rather propagated vegetatively by tillering. Carotene contents of the hybrids and both parents were quantified using high-performance liquid chromatography (HPLC). The contents of all edible parts of the hybrids were approximately seven times higher than those of either parent. These results indicate that the hybrid is a new and carotene-rich vegetable ofAlliumspecies. 相似文献
20.
Intergeneric hybrids were obtained between Sandersonia aurantiaca and Littonia modesta using ovule culture. The embryos were rescued by culturing 14 to 30 day old ovules. The ovules were cultured on modified
KM medium for varying lengths of time until they germinated. After germination the embryo-derived-plantlets were transferred
to modified growth regulator-free MS medium on which they developed tubers and became quiescent. The quiescent tubers could
be successfully transferred to soil. The hybrid nature of both the putative Sandersonia × Littonia and the Littonia × Sandersonia hybrids was indicated by flow cytometry that showed the hybrid plants had a DNA content midway between that of the two parents.
Mitotic and meiotic chromosome counts from S. aurantiaca, L. modesta and the hybrids gave chromosome numbers of (2n=) 24, 22 and 23 respectively. Morphological analyses of the leaves and flowers
showed that the hybrids displayed features that were intermediate between both parents. Hybrids were male and female sterile.
No morphological differences were observed between the two hybrids.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献