共查询到18条相似文献，搜索用时 156 毫秒
1.
棉花曲叶病研究进展 总被引：1，自引：0，他引：1
棉花曲叶病(Cotton leaf curl disease,CLCuD)是棉花上的重要病害,在巴基斯坦和印度已造成严重危害。已发现7种双生病毒与亚洲和非洲发生的CLCuD相关,在田间这些双生病毒常复合侵染且病毒基因组重组现象较为普遍。CLCuD伴随不同类型的小分子DNA,其中新型卫星DNA分子——DNAβ与CLCuD致病性紧密相关,是诱导田间典型症状所必需的。重组导致CLCuD的病毒多样化,而DNAβ分子能与不同双生病毒互作,这可能是引起CLCuD流行的重要原因。本文介绍了CLCuD的分布与危害、病害病原致病因子的发现及CLCuD病害复合体各组份之间的互作、病毒及小分子DNA的变异与进化关系、病害流行及防治等方面的研究进展。 相似文献
2.
烟草曲茎病毒/DNAβ病害复合体研究进展 总被引：1，自引：1，他引：0
烟草曲茎病毒(Tobacco curly shoot virus, TbCSV)是从云南烟草上分离的双生病毒科Geminiviridae菜豆金色花叶病毒属Begomovirus的一个新种,在番茄和烟草上可引起严重的曲叶病。该病毒与伴随卫星DNAβ形成的病害复合体在病毒致病性、DNAβ与辅助病毒的伴随关系及调控症状的作用等方面均与其它双生病毒病害复合体不同,对其进化起源的研究表明,TbCSV在进化中介于需要卫星的双生病毒和真正的单组分双生病毒之间,因而针对TbCSV病害复合体的研究对于解读双生病毒的起源和遗传进化机制具有重要意义。作者从TbCSV病害复合体的发生危害、基因组结构、伴随小分子DNA、各DNA组分互作以及起源进化等方面综述了该病毒病害复合体的研究进展。 相似文献
3.
为了明确四川米易赛葵上的双生病毒种类及复合侵染情况,基于滚环扩增PCR（RCA-PCR）技术,利用双生病毒DNA-A、DNA-B及卫星DNA的通用引物对四川米易表现黄脉症状的12个赛葵样品进行了检测,并对其序列进行分析。双生病毒的检出率高达92%;共检出云南赛葵黄脉病毒（Malvastrum yellow vein Yunnan virus,MYVYNV）、赛葵黄脉病毒（Malvastrum yellow vein virus,MYVV）及中国番茄黄化曲叶病毒（Tomato yellow leaf curl China virus,TYLCCNV）等3种双生病毒,复合侵染率高达67%;11个阳性样品中共检测到两类卫星DNAβ分子,分别与MYVYNV伴随的DNAβ（MYVYNB-[Y160],98.4%～98.7%）和MYVV伴随的DNAβ（MYVB-[Y197],98.5%～98.7%）的序列相似性最高;未扩增到DNA-B及DNAα分子。表明病毒DNA-A与卫星DNAβ以病害复合体形式存在,且DNAβ可伴随异源辅助病毒。 相似文献
4.
双生病毒是一类在全球范围内危害严重的单链环状DNA病毒。本研究从2017年采自云南的胜红蓟样品中(YN2017)获得了一条菜豆金色花叶病毒属病毒和一条β卫星的全基因组序列。该双生病毒的全长序列与烟草曲茎病毒的相似性最高,为99.60%,确定为烟草曲茎病毒的分离物。YN2017β卫星与中国胜红蓟黄脉β卫星的同源性最高,为90.8%。进一步分析发现,该β卫星的卫星保守区域(SCR)至富含腺嘌呤区(A-rich区)之间约1 kb的序列与中国胜红蓟黄脉β卫星相应序列的相似性高达97.2%;其包含的A-rich区上游与SCR之间约300 bp的序列与中国胜红蓟黄脉β卫星相应序列的相似性仅为70.2%,而与烟草曲茎β卫星相应序列的相似性最高,为97.3%。重组分析发现,所分离的β卫星是由中国胜红蓟黄脉β卫星和烟草曲茎β卫星重组产生。这是首次在中国发现由不同的β卫星重组产生的β卫星分子。 相似文献
5.
从中国福建省表现曲叶和脉突症状的赛葵上分离到病毒分离物FJ1~FJ6。利用菜豆金色花叶病毒属病毒的特异性简并引物PA/PB,在所有6个分离物中都分离到了约500bp长度的病毒部分DNA片段。这些DNA片段与已报道的广东赛葵曲叶病毒(Malvastrum leaf curl Guangdong virus,MLCuGdV)的核苷酸序列同源性高达90%-93%。随机挑选FJ3分离物进行全基因组DNA的克隆测序。结果表明,FJ3 DNA全长2765个核苷酸,具有典型的双生病毒科病毒的基因组结构特征,与MLCuGdV的同源性为92.9%,表明FJ3是MLCuGdV的一个分离物。系统进化分析表明,除了MLCuGdV,FJ3与其它赛葵上分离到的双生病毒的亲缘关系都较远,而与分离自中国南方番木瓜上的双生病毒聚成簇,有较近的亲缘关系。进一步比较分析各蛋白编码的氨基酸序列发现,FJ3可能是一个种间重组分子,它可能是由中国番木瓜曲叶病毒或广东番木瓜曲叶病毒和另外的未知病毒重组产生的。 相似文献
6.
甘薯是重要的粮食作物和食品加工及工业原料。我国是世界上最大的甘薯生产国。病毒病是甘薯上的重要病害,目前世界上已报道的侵染甘薯的DNA病毒主要归属于双生病毒科Geminiviridae和花椰菜花叶病毒科Caulimoviridae。近年来,双生病毒等DNA病毒严重影响我国甘薯的产量、品质以及食品加工产业。本文简介了甘薯在我国的重要地位和种植情况;具体介绍了侵染甘薯的菜豆金色花叶病毒属Begomovirus、玉米线条病毒属Mastrevirus及杆状DNA病毒属Badnavirus的病毒特征、分子变异、分类现状和检测方法。结合甘薯生产的实际情况,提出了目前甘薯DNA病毒研究中存在的问题及思考。本文旨在为我国甘薯DNA病毒病的综合防控提供理论依据。 相似文献
7.
双生病毒作为全球范围内引起作物严重病害的单链DNA病毒，编码多种多功能蛋白，其帮助自身完成复制、转录、组装、移动和致病等生命过程，其中双生病毒的复制增强蛋白C3/AC3在病毒侵染初期可促进病毒复制，提高病毒积累水平以帮助其快速建立侵染。为深入解析C3/AC3蛋白的功能和双生病毒致病机制，该文概述双生病毒C3/AC3蛋白的基本生物学特性及其在病毒侵染过程中的功能，重点阐述C3/AC3蛋白与其他蛋白及寄主因子互作以促进病毒复制的分子机制，同时针对双生病毒复制机制和C3/AC3蛋白的功能解析进行展望。 相似文献
8.
江苏朱槿上分离到的木尔坦棉花曲叶病毒基因组结构特征分析 总被引：1，自引：0，他引：1
2012年5月,江苏南京朱槿上出现一种新的病毒病害,病株表现明显的叶片上卷、叶脉肿大、叶背伴有耳突、植株矮缩等症状。根据其症状及介体发生状况,对其伴随的病毒种类进行研究,结果发现采集的46份典型症状样品体内均可以检测到粉虱传双生病毒,对其基因组DNA-A组分克隆测序后发现其全长2 736 bp,编码6个ORF,BLAST分析显示该病毒与木尔坦棉花曲叶病毒同源性最高(99.9%),是木尔坦棉花曲叶病毒的一个分离物。病样中同时还检测到伴随DNAβ卫星分子,测序结果显示该卫星全长1 346 bp,编码1个ORF,BLAST及聚类分析结果显示该β卫星与木尔坦棉花曲叶病毒β卫星同源性最高(99.7%),为木尔坦棉花曲叶病毒β卫星的一个分离物。这是木尔坦棉花曲叶病毒首次在长江流域的报道。 相似文献
9.
高通量测序技术(next-generation sequencing,NGS)平台提供了一种高效、快速、低成本、深度测序DNA的解决方案,2009年该技术开始被应用于植物病毒学领域,包括新病毒的发现,病害病原的鉴定,病毒基因组多样性及进化的研究,显著加快了植物病毒学的发展进程。迄今,应用高通量测序技术已经成功鉴定了上百种新的植物病毒和类病毒。双生病毒是一类对多种作物造成毁灭性危害的DNA病毒,多发生于草本作物。然而利用高通量测序技术,从柑橘、葡萄、苹果和桑树等多种多年生木本植物中检测到了新的双生病毒,显示出了高通量测序技术所独有的、传统检测技术所不具备的优势。本文围绕高通量测度技术在植物病毒学领域的应用进行概述,重点阐述NGS用于检测木本植物双生病毒的几个实例。 相似文献
10.
双生病毒是一类在全世界范围内广泛发生的单链环状DNA病毒。本文对从湖南采集到的6例(洋姜、番茄、萝卜、赛葵、甘薯、牵牛花)疑似双生病毒侵染的植物叶片进行了分子鉴定。利用滚环扩增技术(RCA)对样品DNA进行扩增,分别对其RCA产物进行酶切,并将酶切得到的片段测序后进行BLAST比对,结果显示番茄样品中的病毒分离物与番茄黄化曲叶病毒相似性最高(99%),牵牛花样品中的病毒分离物与甘薯卷叶病毒相似性最高(99%),证明这两个分离物是单组分DNA-A双生病毒。这是在湖南省首次发现并报道双生病毒的全核酸序列。 相似文献
11.
用滚环扩增技术检测双生病毒 总被引：2，自引：0，他引：2
本文报道了利用滚环扩增技术(rolling circle amplification,RCA)放大双生病毒的靶核酸信号,进而利用限制性内切酶片段长度多态性(RFLP)检测不同类型双生病毒的新技术体系。研究结果表明,利用RCA-RFLP对于已知的单组份和双组份以及带有卫星的菜豆金色花叶病毒属病毒都能够有效检测,对采自浙江杭州地区的田间样品进行RCA-RFLP检测的结果与利用简并引物PA和PB进行PCR扩增的结果相吻合。相比传统的PCR检测方法,用RCA-RFLP体系检测双生病毒灵敏性更高,操作更简便。 相似文献
12.
<正>番茄黄化曲叶病毒(Tomato yellow leaf curl virus,TYLCV)在世界范围内可危害多种作物,造成植株矮化、叶片皱缩变形、局部黄化等症状。该病毒自1964年首次报道以来已蔓延至世界多地。在我国2006年上海首次报道该病毒[1],随后江苏、山东、安徽、北京、河北、天津等地相继报道,危害严重。TYLCV为双生病毒科(Geminiviridae)菜豆金色花叶病毒属(Begomovirus)成员,基因组为单组 相似文献
13.
A vein-yellowing disease of Ageratum conyzoides in Singapore was shown to be caused by a geminivirus, here named ageratum yellow vein virus (AYVV), which was transmitted by the whitefly Bemisia tabaci but not by inoculation with sap or through seed. AYVV particles (30 × 20 nm) are serologically related to those of other whitefly-transmitted geminiviruses, and reacted with some monoclonal antibodies elicited by particles of African cassava mosaic or Indian cassava mosaic geminiviruses. However, the epitope profile of AYVV differed from the profiles of these viruses, and from those of geminiviruses from vein yellowing-affected A. conyzoides from India and from yellow leaf curl-affected tomato from either Singapore or India. The results provide further evidence of antigenic differences among geminiviruses that cause similar diseases in the same plant species in different geographical regions. 相似文献
14.
RNA-silencing suppressors of geminiviruses 总被引：2，自引：0，他引：2
15.
Tomato leaf curl geminivirus in Australia: occurrence, detection, sequence diversity and host range 总被引：5，自引：0，他引：5
The occurrence of whitefly transmitted geminiviruses in Australia was studied using a mixed DNA probe capable of detecting a range of distinct geminiviruses. The only geminivirus species detected was Tomato leaf curl virus (TLCV), which is spread across a vast geographical region of far-northern coastal Australia, an area inhabited by the Australasian-Oceania biotype of Bemisia tabaci . The newly introduced silverleaf whitefly, B. tabaci biotype B, forms high population densities in the eastern coastal region of Queensland and is currently located approximately 150 km from the nearest known TLCV-infected area. The viral host range appeared to be narrow and of 58 species of crop plants and weeds inoculated using the B biotype, only 11 became infected with the virus, including five that did not show foliar symptoms. A DNA fragment of 694 nt, including the complete C4 open reading frame (ORF), the overlapping N-terminal part of the C1 ORF and the viral iterons involved in replication, was amplified from 11 TLCV field isolates and sequenced. Sequence analysis revealed an overall sequence variation of up to 14% in this region, as well as the presence of distinct viral iterons. 相似文献
16.
Genomic characterization using nonradioactive probes, polymerase chain reaction with degenerate primers for whitefly transmitted geminiviruses and nucleotide sequencing were used to describe a new bipartite geminivirus, associated with dwarfing and leaf curling of tomatoes and peppers in Jamaica. Partial DNA-A and DNA-B clones were obtained. DNA sequence analysis showed that tomato and pepper samples have a similar geminivirus associated with them. Nucleotide sequence identity > 92% between the common regions of DNA-A and DNA-B confirmed the bipartite nature of the Jamaican geminivirus isolates. Nucleotide sequence comparisons of DNA-A and DNA-B with those of geminiviruses representing the major phylogenetic groups of Western Hemisphere geminiviruses showed the greatest similarity to potato yellow mosaic virus and members of the Abutilon mosaic virus cluster of geminiviruses. This new virus is given the name tomato dwarf leaf curl virus (TDLCV) because of the dwarfing and leaf curling symptoms associated with infected tomato plants. Polymerase chain reaction and Southern hybridization showed mixed infections of TDLCV with tomato yellow leaf curl virus from Israel in 16% of the field samples of tomatoes and peppers. 相似文献
17.
A. Ouattara F. Tiendrébéogo P. Lefeuvre M. Hoareau S. Claverie A. Allibert F. Chiroleu E.V. Traoré N. Barro O. Traoré J.-M. Lett 《Plant pathology》2020,69(2):379-392
In West and Central Africa, as in many regions of the world, vegetables are severely affected by geminivirus diseases. In Burkina Faso, observation of various virus-like symptoms, especially on tomato, suggests the involvement of several geminiviruses and underlines the pressing need for additional information on their diversity, distribution, prevalence and host plant reservoirs. Large-scale surveys conducted in Burkina Faso confirmed the presence of tomato (yellow) leaf curl diseases (ToLCD-TYLCD) and geminiviruses in all localities with mean prevalences of 25% and 45%, respectively. Five geminiviruses including four begomoviruses (pepper yellow vein Mali virus (PepYVMLV), tomato leaf curl Burkina Faso virus, tomato leaf curl Mali virus and tomato leaf curl Ghana virus) and a dicot-infecting mastrevirus (chickpea chlorotic dwarf virus) were characterized on tomato. In addition, PepYVMLV and cotton leaf curl Gezira virus (CLCuGeV) were characterized on pepper and okra, respectively, in combination or not with alphasatellites and betasatellites for CLCuGeV. The most severe, prevalent and widely distributed virus on vegetables was PepYVMLV, which was characterized for the first time in combination with a genetically divergent DNA-B component that may constitute a key factor of PepYVMLV pathogenicity. Of the eight weeds identified as potential reservoir hosts of begomoviruses, four host PepYVMLV. The results confirm the importance of geminivirus diseases on vegetable crops in Burkina Faso and highlight the complex association of geminiviruses and satellites. The detection of begomoviruses in weeds growing close to crops points to the increasing necessity to consider reservoir plants and virus communities in the control of virus diseases. 相似文献
18.
ABSTRACT Geminiviruses are a group of single-stranded DNA viruses that cause major losses on a number of important crops throughout the world. Bean golden mosaic virus (BGMV) is a typical bipartite, whitefly-transmitted geminivirus that causes a severe disease on beans (Phaseolus vulgaris) in the Western Hemisphere. The lack of natural resistance to geminiviruses has led to attempts to engineer resistance, particularly through the use of pathogen-derived resistance strategies. The rep gene contains several conserved domains including nucleoside triphosphate (NTP)-binding and DNA-nicking domains and is the only geminiviral gene necessary for replication. Previous analysis by our group and others has demonstrated that the NTP-binding and DNA-nicking domains are necessary for geminiviral DNA replication. The ability of the rep gene and rep gene mutants to interfere with geminiviral DNA replication, when expressed in trans, was examined using a transient assay in a tobacco suspension cell culture system. Wild-type (wt) and mutant rep genes were cloned into plasmids under the control of the cauliflower mosaic virus 35S promoter for in planta expression and coinoculated into tobacco cells with infectious clones of various geminiviruses. The wt rep gene from BGMV-GA was able to support replication of BGMV-GA DNA-B. Several different rep gene mutants, with function-abolishing mutations in the NTP-binding or DNA-nicking domains, were potent trans-dominant inhibitors of geminiviral DNA replication. 相似文献