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SDIC在马铃薯脱毒组培苗开放式快繁生产中的应用试验研究 总被引：1，自引：0，他引：1
在马铃薯脱毒组培苗的快繁生产过程中常会出现污染问题,马铃薯组培工厂化发展的瓶颈是解决试管苗快繁过程的污染问题。在马铃薯脱毒组培苗的培养基中添加安全、稳定、光谱杀菌剂二氯异氰尿酸钠（SDIC）,从不同浓度的SDIC灭菌效果、培养基中加入SDIC是否高压灭菌、SDIC加入不同培养基对瓶苗生长的影响、SDIC对试管薯诱导的影响等方面进行试验,结果表明,一定浓度SDIC能有效降低脱毒试管苗组培快繁的污染率,不影响瓶苗的正常生长。采用SDIC添加到培养基中,可进行开放式快繁,进行高效、规模化生产组培苗。 相似文献
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草莓组培快繁及叶片诱导植株再生的研究 总被引：7，自引：1，他引：7
以4个草莓品种的匍匐茎尖为起始材料，试验以MS为基本培养基附加不同激素草莓茎尖诱导植株再生、增殖、生根的培养基，确立了草莓组培快繁技术方法；又以4个草莓品种的组培苗叶片为外植体，探讨了不同激素配比、基因型对诱导草莓不定芽再生的影响；通过进一步试验，以MS＋B5有机为基本培养基附加6－BA2．253mg/L、IAA1．752mg/L，探讨草莓不同基因型及不同外植体对诱导不定芽再生的影响，初步建立了一个有效的、较高频率的芽再生系统，也为基因的转化找到一个较好的受体材料。 相似文献
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金叶络石组培快繁技术体系的建立 总被引：4，自引：0，他引：4
以金叶络石[Trachelospermum asiaticum 'Ougonnishiki']带腋芽的茎段为外植体,研究了植物生长调节剂种类及浓度对金叶络石组培苗增殖及生根的影响,建立金叶络石组培快繁技术体系。结果表明:金叶络石组培苗适宜继代增殖培养基为MS+BA3.0mg/L+NAA0.05mg/L,增殖系数为7.63;适宜生根培养基为1/2MS+IBA0.05mg/L,生根率达到95%以上,根数5.2,根长4.4cm;采用"塑料杯单株一步移栽技术",移栽成活率97%。 相似文献
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本试验以大田甜叶菊为试验材料,通过研究不同外植体、不同激素种类和浓度配比对丛生芽诱导、增殖培养及不定根诱导的影响,建立了甜叶菊组培快繁体系。结果表明:带腋芽茎段是甜叶菊组培快繁最适外植体;诱导丛生芽的最佳培养基为MS+6-BA 1.5 mg/L+NAA 0.1 mg/L,平均诱导率为88.1%;继代增殖培养基为MS+6-BA 0.5 mg/L+KT 4.0 mg/L+NAA 0.05 mg/L+GA30.2 mg/L,单芽平均增殖个数为4.1;不定根最适诱导培养基为1/2 MS+NAA 0.2 mg/L,生根率为95%;组培苗移栽成活率达70%以上。本试验研究结果为甜叶菊组培苗的产业化生产提供了一定的理论基础。 相似文献
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《分子植物育种》2017,(1)
本研究以野生疏花唇柱苣苔叶片为外植体进行组培快繁研究,并利用流式细胞术对组培苗进行遗传稳定性分析。研究表明,最佳初代诱导培养基为MS 6-BA 0.5 mg/L和NAA 0.1 mg/L;最适继代增殖培养基为MS 0.5 mg/L 6-BA;最适生根培养基为1/2 MS添加15 g/L蔗糖和0.3 mg/L IBA。所获得的组培苗在无土栽培基质中移栽驯化成活率达到95%以上。组织学切片检测表明,在本实验所用激素浓度组合和培养条件下,以疏花唇柱苣苔叶片为外植体所形成芽体均为器官发生方式形成。流式细胞术检测表明组培苗倍性没有变化,但基因组大小与母本相比发生了11%的增加;母本苗和组培再生苗具有相同的染色体数目(2n=36),植株形态特征上也没有明显差异。 相似文献
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桃是中国主要栽培水果之一,面积和产量均居世界首位,但生产中还没有大面积推广使用无性系抗性砧木,主要原因是桃的无性系砧木的繁殖技术,包括组培快繁技术还不完善。桃的组培快繁技术可用于脱毒、无性系砧木生产、种质资源离体保存及转基因研究等方面,但桃的组织培养相对其它果树较为困难,且不同的品种之间培养条件差异较大。为了总结分析桃组培快繁过程中出现的关键问题,本综述对桃的组培快繁过程中外植体建立、增殖、伸长、生根和炼苗移栽等环节所涉及的关键影响因子,如培养基中的养分、激素种类和浓度、碳源种类和浓度、最佳培养条件、不同的添加物等,进行了全面的分析总结,提出了桃组培快繁基本技术方案和研究思路,为桃的组织培养相关研究提供参考。 相似文献
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<正>植物组织培养技术作为一种常规快繁技术,在花卉种苗生产中已得到广泛应用。然而,从大量的组培瓶苗到获得可出圃的穴盘苗,温室炼苗移栽是一个极为关键的环节,直接关系到花卉种苗的产量及品质。笔者将花卉组培苗温室炼苗移栽及栽后养护介绍如下。组培苗选择选取转接到生根培养基上的组培苗进入温室进行炼苗,所选组培苗必须无污染,株高及叶片大小适宜,叶片数为5~8片,根系为乳白色,根系长度为0.5~1cm。组培苗状态的选取因种类、 相似文献
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百合科十二卷属玉露的组培快繁关键技术研究 总被引：4，自引：0，他引：4
为建立十二卷属多肉花卉玉露的快繁技术体系,以当年抽生的花序为外植体,以MS为基本培养基,研究不同的激素配比对外植体的诱导、分化、增殖、壮苗与生根的影响,建立适合玉露离体快繁的技术体系,最佳启动培养基为MS 6-BA 2.0 mg/L NAA 0.02 mg/L,愈伤组织诱导及分化培养基MS 6-BA 0.5 mg/L KT 0.5 mg/L NAA 0.01 mg/L,愈伤组织诱导率达87.1%,丛芽分化率达52.3%,生根培养基为1/2MS IBA 0.5 mg/L NAA 0.1 mg/L AC 0.5 g/L。试管苗生根率达97.5%,炼苗成活率达90%以上。本项研究结果可以在不损伤母本的基础上实现玉露的人工组培快繁,通过此项技术可以实现玉露种苗的规模化生产。 相似文献
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以切花玫瑰红唇的带腋芽茎段为材料,研究HgCl2灭菌时间、茎段粗细度、基础培养基、6-苄氨基腺嘌呤（6-BA）、α-萘乙酸（NAA）、赤霉素（GA3）、吲哚丁酸（IBA）、吲哚乙酸（IAA）、活性炭（AC）对切花玫瑰组织培养的影响,分析筛选出了一套能够育出具有优良综合素质切花玫瑰种苗的组织培养技术,建立了切花玫瑰的组织培养快繁技术体系。结果表明：不同因素对切花玫瑰组织培养的影响较大,尤其是对玫瑰的分化、增殖、生根和生长等植物形态发育的影响;外植体的最佳灭菌方法是用0.20% HgCl2灭菌8min;初代的适合培养基为MS+6-BA 1.0mg/L+NAA 0.2mg/L+GA3 2.0mg/L;增殖继代的最佳培养基为MS+6-BA 2.0 mg/L+NAA 0.01mg/L+GA3 1.0mg/L;生根的最佳培养基为1/2 MS+6-BA 0.5mg/L+IBA 1.0mg/L+AC 0.1%。该技术可推广应用于切花玫瑰工厂化组织培养育苗。 相似文献
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St Augustinegrass [Stenotaphrum secundatum (Walt.) Kuntze] is an important warm season turf and pasture grass. In vitro tissue culture of St Augustinegrass could serve as an important mean for its improvement through genetic transformation as well as induced somaclonal variation. To optimize tissue culture conditions for plant regeneration of St Augustinegrass, tissue culture responses of 11 explant tissues and four callus induction/subculture media have been examined. Embryogenic calli with regeneration potential were observed on cultures of early immature embryo [3 days after pollination (DAP)], immature embryo (7–14 DAP), and shoot base of young seedlings. The addition of benzyladenine (BA) in the callus induction/subculture medium enhances callus regeneration ability and does not harm callus induction for immature embryos. The best response came from 7 to 14 DAP immature embryo on MS medium containing 1 mg/l 2,4‐dichlorophenoxyacetic acid and 0.5 mg/l BA. The callus induction and regeneration rates were 97.7% and 47.6% respectively. However, BA supplement reduced callus formation and failed to enhance regeneration for young leaf bases. Scanning electron microscopy revealed that plant regeneration of St Augustinegrass is via somatic embryogenesis. 相似文献
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Formation of embryo autonomy of strawberry, plant regeneration fro membryo components, plant freezing conditions in vitro and the possibility to differentiate objectively genotypes by freezing them in vitro and in vivo were studied to create strawberry screening technology in vitro for cold resistance. It was established that autonomy of strawberry embryos manifests itself not earlier than on 14–16th day after pollination and full autonomy is reached on 20–22nd day. Plants regenerated from 26 days old embryos grew most intensively. At the highest rate strawberry plants regenerated
from an isolated embryo axis on MS medium without phytohormones, and from rescued cotyledons x on the medium with 1.0 BA and
0.5 NAA. The temperature interval, at which genotypes differentiated according to cold resistance in vitro, was -8 to 12 °C. Differentiation of strawberry genotypes according to this character conformed to their differentiation in vivo, provided hardening proceeded not less than 21 days. The correlation between cold resistance in vitro and in vivo reached 0.93. Domination of cold resistance manifested itself in strawberry seedlings from various crossing combinations.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
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P. Bertin J.-P. Busocoro J.-P. Tilquin J.-M. Kinet J. Bouharmont 《Plant Breeding》1996,115(3):183-188
A field experiment was designed to compare control with tissue culture-derived plants of rice, and to study the effects of natural selection pressure applied to the first generation (R0) of in vitro plants on the performance of their progeny. Control- and R0 in vitro plants of four rice varieties from middle altitude in Burundi, i.e. Facagro 57′, Facagro 76′, ‘Kirundo 3’ and ‘Kirundo 9’, were cultivated at four different altitudes (800, 1380, 1650 and 1900 m). Several parameters concerning tillering capacity, plant height development and seed production were measured. The means for most measured parameters were higher in control plants than in the in vitro plants, while the higher variation coefficients and most extreme values were usually found in the in vitro plants. For each variety, the 20 control plants and 20 in vitro plants having the highest production were selected at each altitude of 1380 and 1650 m, and their progenies were cultivated on the same site at 1580 m. Among the plants arising from 1650 m, most maximum values, higher variation coefficients and, in some cases, higher means were found in the in vitro plants. In contrast, among the plants arising from 1380 m, higher means most usually corresponded to control plants, while the tendency for maximum values and variation coefficients were unclear. These results indicate the positive effect of natural selection pressure applied in the first generation to plants rising from tissue culture. They also demonstrate that considerable variation may be generated in vitro. The interest for plant breeding purposes is discussed. 相似文献
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药用植物裸花紫珠的组织培养与快速繁殖研究 总被引：2，自引：2，他引：0
以种子为外植体,MS为基本培养基,通过比较不同植物生长调节剂种类和浓度配比、培养条件以及生根苗的移栽基质,建立裸花紫珠组织培养快速繁殖体系。结果表明：外植体表面消毒以75%酒精预处理10 s,再用0.1% HgCl2浸泡10 min,效果最好;种子在MS基本培养基上萌发;丛生芽继代增殖的最适温度为28℃,最适光照强度为1500 lx;培养基MS+ 6-BA 2.00 mg/L+ NAA 0.05 mg/L适宜继代增殖,30天的增殖系数为10.87;培养基MS+ NAA 0.50~0.75 mg/L适宜诱导生根,生根率100%;生根苗移栽于河沙、珍珠岩和表土(1:1:1)的混合基质中,成活率96%,高生长量最大。运用该组培快繁技术,可以实现裸花紫珠的工厂化育苗。 相似文献
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多胺及稀土对欧石楠诱导分化的影响 总被引：1，自引：1，他引：0
为探讨多胺(ployamines, PAs)及稀土元素(rare-earth elements)在植物组织培养应用中的理论基础和实践意义,以欧石楠继代瓶苗为外植体,采用组织培养的方法,研究不同浓度的多胺及硝酸铈和硝酸镧对其继代瓶苗诱导分化的作用。在基本培养基(1/2 WPM+ZT 1.0 mg/L+IBA 0.3 mg/L)中添加1.0 mg/L的亚精胺(Spd),欧石楠的平均分化倍数、平均分化苗高及胚性愈伤组织诱导率分别达到3.89、3.11 cm、98.30%;在基本培养基中添加1.0 mg/L的硝酸铈[Ce(NO3)3],欧石楠的平均分化倍数、平均分化苗高及胚性愈伤组织诱导率分别为6.9、4.0 cm、98.70%;在基本培养基中添加0.5 mg/L的硝酸镧[La(NO3)3],欧石楠的平均分化倍数、平均分化苗高及愈伤组织诱导率分别为5.5、3.89 cm、100%,这3组试验,显著性方差分析及多重比较结果显示,该处理相对于其他处理均达到极显著水平。低浓度的多胺及稀土元素对欧石楠继代瓶苗的分化及生长具有促进作用,而高浓度则起抑制作用。 相似文献
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Summary Seventy-two plants regenerated from leaf-derived calli of a single plant of Lotus corniculatus have been evaluated for several morphological and agronomical traits. The analysis of selfed and polycross progenies of the regenerants indicates that the variation among regenerants was, at least in part, of genetic origin. Most of the mutations induced by tissue culture were recessive and were detected only after sexual propagation. Although in vitro culture had a depressive effect for most of the traits, the selfed progenies of 2 regenerants displayed higher values for leaflet width and seed yield than the selfed progeny of the initial plant. However the somaclonal variation did not increase the variation for any trait with respect to the variation of the donor cultivar of the initial plant. 相似文献
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以露薇花种子无菌播种苗和穴盘播种苗为试验材料,比较两种条件下种子发芽情况,并将两种幼苗作为外植体,移入初代培养基内进行诱导培养和继代增殖。运用主成分分析法和相关性分析法,并通过建立拟合生长曲线,量化评价不同播种方式幼苗离体培养下生长情况。结果表明,穴盘播种发芽率较无菌播种发芽率高,幼苗根系发达,但苗离体培养污染率较高;离体无菌培养更有利于露薇花植株生长发育的进行,可将穴盘发芽幼苗灭菌后移植进培养基培养;继代培养穴盘播种和无菌播种苗之间无差异;继代培养3次,单瓶接种两株植株,植株长势最好,萌芽数,萌芽均高,增殖倍数高。通过本次实验,为露薇花高效生产繁殖提供理论依据,从而为西北地区新优特异花卉的引进,提供可行的途径方法。 相似文献