共查询到20条相似文献，搜索用时 31 毫秒
1.
Isolation of infectious bovine rhinotracheitis virus from the soft-shelled tick, Ornithodoros coriaceus 总被引：1，自引：0，他引：1
Infectious bovine rhinotracheitis virus was isolated from the soft-shelled tick (Ornithodoros coriaceus). Serological identification of the isolated viruses was confirmed by restriction endonuclease digestion of purified virus DNA. These isolations indicate that the soft-shelled tick may be a vector for infectious bovine rhinotracheitis virus. This may be the first reported isolation of mammalian herpesvirus from an arthropod vector. 相似文献
2.
Transmission of equine influenza virus to dogs 总被引：1，自引：0，他引：1
Crawford PC Dubovi EJ Castleman WL Stephenson I Gibbs EP Chen L Smith C Hill RC Ferro P Pompey J Bright RA Medina MJ Johnson CM Olsen CW Cox NJ Klimov AI Katz JM Donis RO 《Science (New York, N.Y.)》2005,310(5747):482-485
Molecular and antigenic analyses of three influenza viruses isolated from outbreaks of severe respiratory disease in racing greyhounds revealed that they are closely related to H3N8 equine influenza virus. Phylogenetic analysis indicated that the canine influenza virus genomes form a monophyletic group, consistent with a single interspecies virus transfer. Molecular changes in the hemagglutinin suggested adaptive evolution in the new host. The etiologic role of this virus in respiratory disease was supported by the temporal association of rising antibody titers with disease and by experimental inoculation studies. The geographic expansion of the infection and its persistence for several years indicate efficient transmission of canine influenza virus among greyhounds. Evidence of infection in pet dogs suggests that this infection may also become enzootic in this population. 相似文献
3.
HAN Xian-jie WANG Jun-wei 《中国农业科学(英文版)》2005,4(8):634-640
The DNA of duck plague virus （DPV） thymidine kinase （TK） gene was cloned and sequenced from a vaccine virus in the study. Degenerate oligonucleotide primers for the consensus site of herpesvirus UL24, TK, and glycoprotein H（gH） gene were used in the polymerase chain reaction （PCR） to amplify DNA product with 3 741-base-pairs （bp） in size. DNA sequence analysis revealed a 1 077-base-pairs （bp） open reading frame （ORF） encoding a 358 amino acid polypeptide homologous to herpesvirus TK proteins. The predicted TK protein shared 31.2, 41.3, 35.7, 37.4, and 28.4% identity with herpes simplex virus typel, equine herpesvirus type 4, Marek＇s disease virus 2, herpesvirus turkey, and infectious laryngotracheitis virus, respectively. Comparison of the amino acid sequences of other herpesvirus TK proteins showed that these proteins were not conserved on the whole, otherwise the portion of the TK proteins corresponding to the nucleotide binding domain and the nucleoside binding site were highly conserved among herpesvirus. Comparison with the amino acid sequences of the conserved nucleotide and nucleoside binding domains of other eleven herpesvirus TK proteins to the predicted DPV peptide confirmed its identity as the DPV TK protein. 相似文献
4.
SW Lee PF Markham MJ Coppo AR Legione JF Markham AH Noormohammadi GF Browning N Ficorilli CA Hartley JM Devlin 《Science (New York, N.Y.)》2012,337(6091):188
Recombination between herpesviruses has been seen in vitro and in vivo under experimental conditions. This has raised safety concerns about using attenuated herpesvirus vaccines in human and veterinary medicine and adds to other known concerns associated with their use, including reversion to virulence and disease arising from recurrent reactivation of lifelong chronic infection. We used high-throughput sequencing to investigate relationships between emergent field strains and vaccine strains of infectious laryngotracheitis virus (ILTV, gallid herpesvirus 1). We show that independent recombination events between distinct attenuated vaccine strains resulted in virulent recombinant viruses that became the dominant strains responsible for widespread disease in Australian commercial poultry flocks. These findings highlight the risks of using multiple different attenuated herpesvirus vaccines, or vectors, in the same populations. 相似文献
5.
Recombination of influenza A viruses of human and animal origin 总被引：11，自引：0，他引：11
E D Kolbourne 《Science (New York, N.Y.)》1968,160(823):74-76
Simultaneous infection of the allantoic sac of the chick embryo with influenza A/equine 1/56 and any of three recombinants derived from human influenza viruses produced stable hybrids with antigens from each parent strain. These hybrids contain the hemagglutinin protein of the equine virus and the neuraminidase of the human strains. The experiments demonstrate genetic homology of human and equine influenza A viruses and suggest the possibility of their recombination in nature. 相似文献
6.
Cloning and Sequence of Glycoprotein H Gene of Duck Plague Virus 总被引：9，自引：0，他引：9
HAN Xian-jie WANG Jun-wei MA Bo 《中国农业科学(英文版)》2006,5(5):397-402
The glycoprotein H （gH） gene homologue of duck plague virus （DPV） was cloned by degenerate polymerase chain reaction （PCR） and sequenced. It was located immediately downstream from the thymidine kinase gene （TK）. In addition, the 3＇-end of the gene homologue to herpesvirus UL21 was located downstream from the gH gene. DPV gH gene open reading frame （ORF） was 2 505 bp in length and its primary translation product was a polypeptide of 834 amino acids long. It possessed several characteristics of membrane glycoproteins, including an N-terminal hydrophobic signal sequence, an external domain containing eight putative N-linked glycosylation sites, a C-terminal transmembrane domain, and a charged cytoplasmic tail. Comparison with other herpesvirus revealed identities of 20.2, 25.1, 23.0, 23.0, 26.5 and 26.0% with the gH counterparts of the human herpesvirus virus 1 （HSV1）, equine herpesvirus 4 （EHV4）, bovine herpesvirus 1 （BHV1）, pseudorabies virus （PRV）, gallid herpesvirus 2 （GHV2） and gallid herpesvirus 3 （GHV3）, respectively. 相似文献
7.
A gene for dihydrofolate reductase in a herpesvirus 总被引：13，自引：0，他引：13
J J Trimble S C Murthy A Bakker R Grassmann R C Desrosiers 《Science (New York, N.Y.)》1988,239(4844):1145-1147
8.
Four distinct viruses with double-stranded DNA are known to replicate in Chlorella-like algae symbiotic with hydras and paramecia. An attempt was made to infect a number of cultured Chlorella strains derived from invertebrate hosts with these viruses. One of the viruses, PBCV-1, replicated in two of the algal strains. Restriction endonuclease analysis of the viral DNA showed that the infectious progeny virus was identical to the input virus; thus, Koch's postulates were fulfilled. Viral infection of the two Chlorella strains has allowed the large-scale production of a eukaryotic algal virus and the development of a plaque assay for the virus. 相似文献
9.
YUAN Xiu-fang ZHOU Tao HOU Shao-hua TU Ya-bin PENG Jin-mei WEN Jian-xin QIU Hua-ji WU Dong-lai TONG Guang-zhi 《中国农业科学(英文版)》2011,10(1):126-135
The aim of this study was to determine the genomic evolutionary pattern of virulent equine infectious anemia virus (EIAV)during persistent infection.The evolutionary dynamics of proviral genomes were examined by challenging an EIAV seronegative equine (pony 1) and three EIAV vaccinated equines (ponies 4,7,and 8) with the Chinese virulent strain EIAVL.Ponies I and 7 succumbed to disease and were called progressors,while ponies 4 and 8 lacked clinical symptoms and were considered nonprogressors.Sequences spanning the V3,V4,and V5 hyper-variable regions of the EIAV-L envelope gp90 gene were sequenced from each pony as evolutionary markers of the provirus.The proviral genome of the EIAV-Linoculum evolved during persistent infection and displayed different patterns between EIA progressors and nonprogressors.Inoculum-like variants were isolated from nonprogressors during persistent infection,but only from progressors during acute infection.Variant mutations from nonprogressors were dispersed throughout the sequenced region,while those from progressors were predominantly localized to V3.Humoral immunity and virus variant population selection analyses indicated that immune selection was positive in chronically infected progressors and weak in nonprogressors.In-frame stop codons were frequently localized to a defect "hot spot".The high number of defective variants in nonprogressors may promote disease survival. 相似文献
10.
V S Hinshaw R G Webster W J Bean J Downie D A Senne 《Science (New York, N.Y.)》1983,220(4593):206-208
Influenza A viruses (subtype H1N1), recently isolated from turkeys in different areas of the United States, were determined to be closely related to strains typically associated with pigs. This conclusion was based on comparisons of H1N1 isolates from pigs, humans, ducks, and turkeys with polyclonal and monoclonal antibodies, RNA-RNA competitive hybridization, and replication studies. One of the H1N1 isolates from turkeys caused influenza in a laboratory technician, who displayed fever, respiratory illness, virus shedding, and seroconversion. These results suggest that turkeys as well as pigs are involved in the maintenance of these viruses and their transmission to humans. 相似文献
11.
Russell CA Fonville JM Brown AE Burke DF Smith DL James SL Herfst S van Boheemen S Linster M Schrauwen EJ Katzelnick L Mosterín A Kuiken T Maher E Neumann G Osterhaus AD Kawaoka Y Fouchier RA Smith DJ 《Science (New York, N.Y.)》2012,336(6088):1541-1547
Avian A/H5N1 influenza viruses pose a pandemic threat. As few as five amino acid substitutions, or four with reassortment, might be sufficient for mammal-to-mammal transmission through respiratory droplets. From surveillance data, we found that two of these substitutions are common in A/H5N1 viruses, and thus, some viruses might require only three additional substitutions to become transmissible via respiratory droplets between mammals. We used a mathematical model of within-host virus evolution to study factors that could increase and decrease the probability of the remaining substitutions evolving after the virus has infected a mammalian host. These factors, combined with the presence of some of these substitutions in circulating strains, make a virus evolving in nature a potentially serious threat. These results highlight critical areas in which more data are needed for assessing, and potentially averting, this threat. 相似文献
12.
为建立一种特异和敏感的检测锦鲤疱疹病毒(koi herpesvirus,KHV)潜伏感染的方法,以KHV Sph基因为靶序列设计特异性引物,通过条件优化建立Real–Time PCR检测方法,并且对该方法进行特异性和敏感性评估及临床样品应用研究。结果显示:建立的方法与鲤痘疱疹病毒、金鱼造血器官坏死病病毒和鳗鲡疱疹病毒均无交叉反应,特异性强;最低检出率为42拷贝/μL,灵敏度高。用本方法对63份临床样品进行检测,有锦鲤疱疹病毒病(KHVD)病史的18份样品的检测结果全为阳性,阳性率为100%;从有临床症状的鱼中得到的15份样品的检测结果全为阳性,阳性率为100%;30份无任何临床症状鱼中的6份被检测为阳性,阳性率为20%。本试验中建立的水生动物疱疹病毒潜伏感染快速检测方法,可为潜伏感染KHV的临床检测提供一种快速、敏感和特异的检测手段,可为KHVD的分子流行病学调查和预防提供参考。 相似文献
13.
An endonucleolytic activity has been identified in nuclear extracts of chick embryo bursa and mouse fetal liver cells. The activity introduces a double-strand cut in the vicinity of the recombination site of immunoglobulin joining gene segments. The cleavage occurs at the dinucleotide pair TG-AC. This activity is a good candidate for the putative endonuclease involved in recombination of the immunoglobulin variable, diversity, and joining regions. It is distinct from the endonuclease activities previously reported by others. 相似文献
14.
新型禽流感病毒对家禽养殖业可造成严重经济损失，及时发现和阻断其传播具有重要意义。2022—2023年，本实验室在我国发病鸡群中监测到新型H3N3亚型禽流感病毒的出现和传播。本研究对发病鸡群开展了临床发病情况调查，对分离病毒进行了全基因组演化分析，并对分离的代表性病毒进行了鸡致病性以及空气传播性试验。结果表明：1)H3N3最早于2022年12月在华东地区发生产蛋下降的蛋鸡群出现，随后陆续在华东、华北、东北等多个地区的鸡群中监测到，发病群体主要为产蛋鸡，鸡群发病率高，死亡率低(1%～5%),主要引起产蛋率急性下降(10%～40%)。2)病毒全基因组序列分析显示，H3N3病毒为新型重排病毒，由鸡群中流行的H3N8病毒与H10N3病毒重排产生，6个内部基因来源于H9N2病毒。3)与H3N8病毒类似，新型H3N3病毒对鸡群高度易感，可在鸡鼻甲、气管、肺等呼吸器官中高效复制并排毒，引起呼吸系统严重病理损伤。4)空气传播性结果表明，新型H3N3病毒可在鸡群之间空气传播，而H3N8病毒不能在鸡群间空气传播。综上，新型H3N3禽流感病毒对鸡具有较强的致病性和传播性，有可能成为新的优势流行病毒，对我国家禽... 相似文献
15.
本研究主要依据中兽医理论,围绕中药制剂调动动物自身这一主要法则,以充分发挥中药扶正祛邪、标本兼顾的特色和优势,使中药的组合效应达到最佳。荆防败毒散主要成分为荆芥、防风、羌活、独活、柴胡、麻黄、黄连、连翘等名贵中药经浓缩冷冻干燥提取物。多病因呼吸道病(Multicausal respiratory dis-ease)是指在商品化生产条件下以病毒、细菌、支原体、免疫抑制病病原和不利环境因素等多种原因引起并发或混合感染的呼吸道病。 相似文献
16.
云南地区部分猪场猪呼吸道疾病综合症（PRDC）患病猪群中PRRSV，PCV-2，CSFV，PRV混合感染调查 总被引：2，自引：0，他引：2
针对云南省不同地区不同季节猪呼吸道疾病综合征进行流行病学调查,根据其临床特征,在不同规模养殖场共采集1164份血清样品,利用ELISA和RT-PCR方法检测其猪瘟抗原CSFV,猪繁殖与呼吸综合征抗原PRRSV,猪伪狂犬病PRV gE抗体及猪圆环病毒2型PCV-2特异抗体。结果表明：各季节和不同生长阶段猪群存在一种及两种以上的病毒混合感染,四重感染相对较少。从全年看单独感染占39.10%,PCV-2感染率最高,其次是PRV,PRRSV和CSFV;二重感染占26.13%,以PCV-2+PRV最常见,其次是PCV-2+ PRRSV、PRV+PRRSV;三重感染占8.05%,PCV-2+ PRRSV+PRV最常见;有四重感染出现,仅占1.57%。从季节来看,春季和夏季混合感染最高,分别为76.53%和60.74%,冬季和秋季的混合感染率分别为59.50%和53.55%。从年龄和性别看,育肥猪的混合感染率高于仔猪,分别为68.66%和61.11%,种公猪的感染率高于母猪,分别为70.00%和55.51%。说明4种病毒有单独感染或混合感染,推测其中PCV-2在混合感染中可能充当免疫抑制的角色,混合感染使PRDC症状更明显,死亡率增高。 相似文献
17.
A quantitative bioassay for HIV-1 based on trans-activation 总被引：31，自引：0，他引：31
A bioassay that is based on trans-activation has been developed for the detection and quantitation of the human immunodeficiency virus type 1 (HIV-1). Indicator cell lines were constructed that contain the HIV-1 long terminal repeat ligated to the chloramphenicol acetyltransferase (CAT) gene. Infection of these cells by HIV activates the expression of CAT protein. Isolates of HIV-1 with divergent nucleotide sequences activated the indicator cell lines to a similar extent, approximately 500- to 1000-fold. Human T cell lymphotropic viruses types 1 and 2, equine infectious anemia virus, and herpes simplex virus 1 did not activate the indicator cell lines. Isolates of simian immunodeficiency virus and human T cell lymphotropic virus type 4 activated these cells to a much lesser extent, which suggests that these viruses contain similar, but distinct, trans-activators. This assay can be used for the detection, quantitation, and typing of HIV and for studying the effect of drugs on the replication of HIV in different cellular backgrounds. 相似文献
18.
牛呼吸道疾病综合征相关病毒BVDV、BPIV3、IBRV和BRSV多重PCR检测方法的建立 总被引：2，自引：0，他引：2
根据Genbank发表的牛呼吸道综合征相关病毒中牛传染性鼻气管炎病毒(IBRV)gB基因、牛呼吸道合胞体病毒(BRSV)N基因、牛病毒性腹泻病毒(BVDV)5′-UTR和牛副流感3型病毒(BPIV3)N基因设计特异性引物,建立了在同一体系内同时对4种病毒进行检测的多重PCR方法。该方法可以同时检测出DNA病毒IBRV的长306bp和反转录病毒BPIV3长422bp、BRSV长600bp及BVDV长130bp的特异性片段。本项研究中以已知IBRV、BPIV3、BRSV和BVDV作为参考毒株,对来自内蒙古、辽宁、安徽和吉林4个省、自治区的33例临床样品进行检测。结果表明,合并感染2种病毒较为多见,分别为BVDV和BRSV共感染,BPIV3和BRSV共感染,BPIV3和IBRV共感染,未检测到4种病毒同时感染临床样品。 相似文献
19.
Isolation and propagation of a human enteric coronavirus 总被引：6，自引：0，他引：6
Coronavirus-like particles were found by electron microscopy in stools from infants with necrotizing enterocolitis. Stool samples from these infants as well as control specimens were passaged in cultures of human fetal intestinal organs. Two samples yielded virus-like particles and these have now been passaged 14 times (HEC 14). Gradient-purified HEC 14 strains had typical coronavirus morphology on electron microscopy and contained five major proteins with molecular sizes ranging from 190 to 23 kilodaltons. Infants with necrotizing enterocolitis developed specific antibody to the viral antigens between the acute and convalescent stages of the disease, as shown by examining serum specimens by single radial hemolysis, immunoenzymatic assay, and Western immunoblotting. No cross-reactivity was shown with other coronavirus strains tested, or with the newly isolated viruses of the Breda-Berne group, responsible for calf or horse diarrhea. 相似文献
20.
禽疱疹病毒主要包括α疱疹病毒亚科的鸡马立克氏病毒( Mareks disease virus, MDV)、传染性喉气管炎病毒( infectious laryngotracheitis virus, IBDV )和鸭肠炎病毒( duck enteritis virus, DEV )。作为疱疹病毒家族的成员,禽疱疹病毒基因组庞大,存在多个复制非必需区,可容纳多个外源基因的插入,是表达其他禽类病原抗原基因的理想载体。总结了禽疱疹病毒的构建方法、外源基因表达水平,并对MDV、IBDV和DEV 三种禽疱疹病毒的不同重组病毒疫苗免疫效果评价进行归纳,旨在为畜禽传染病防治提供参考依据。 相似文献