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1.
Viable propagules ofVerticillium albo-atrum andV. dahliae were recovered from potato stems before and after burial in the field during 1968–1971. Of 14 cultivars tested: Red Pontiac and Superior ranked highest in numbers of propagules ofV. albo-atrum. Russet Burbank, Kennebec, and Irish Cobbler ranked highest withV. dahliae, respectively. Survival ofV. albo-atrum in infected stems was evident (5 ? 34 × 103 ppg) even after burial in field soil for 17 months. In general, survival ofV. albo-atrum (47–69%) was higher thanV. dahliae (23 – 61%) following field burial of infected stems for 7 months.  相似文献   

2.
In three years (1994, 1995, and 1996), a total of 100 commercial potato fields in southeastern Idaho were surveyed for soil variables, severity of Verticillium wilt, soil inoculum density ofVerticillium dahliae andColletotrichum coccodes, colonization of stems, root, and tubers byV. dahliae andC. coccodes, and tuber yield, size, and quality. As a generalization, factors related to soil integrity (organic matter, organic nitrogen, and increased nutrient availability) were most closely related to wilt suppression and higher tuber yields, whereas factors related to loss of soil integrity (sodium and reduced nutrient availability) were related to increased wilt and lower tuber yields. In a multiple regression analysis, three independent variables, feeder-root infections byV. dahliae, sodium content in soil, and soil organic content, were significant predictors of tuber yield. With these three factors, this model accounted for 49%, 53%, and 62% of the field variability related to total yield in 1994, 1995, and 1996, respectively. Throughout this investigation,V. dahliae root infections had the most direct effect on tuber yield, which emphasizes the importance of quantifying root infections in epidemiological studies of Verticillium wilt. Based on these results, organic matter may be one factor that can be manipulated for suppression of Verticillium wilt without reducing soil populations of the pathogen.  相似文献   

3.
Soil population levels ofVerticillium dahliae in Ohio were monitored from May–October, 1982–1985, in 15 fields in potato-wheat rotation and two fields in potato monoculture. Population levels in fields in rotation ranged from 0 to 86 microsclerotia/10 g of air-dried soil with average values during each 6-month sampling period of 9.7, 12.9, 9.6 and 19.6 microsclerotia/10 g of soil for 1982, 1983, 1984 and 1985, respectively. In at least 2/3 of the fields sampled each year, soil populations ofV. dahliae peaked in either July or August, regardless of whether fields were under wheat rotation or potato monoculture. Of the 15 fields under potato-wheat rotation, 13 showed a general pattern of increased populations ofV. dahliae in one or both years following cropping to potato. Among those 13, significantly higher populations (P < 0.05) were observed in one wheat field in 1983 and in eight fields in 1985. In the two fields in potato monoculture, one consistently had population levels ofV. dahliae 3–4 times higher than any other field sampled in this study. Factors that may contribute to periodic changes inV. dahliae populations, implications of these changes in interpreting soil population data, and usefulness of a potato-wheat rotation in managingV. dahliae populations in Ohio are discussed.  相似文献   

4.
Studies in the 1950’s indicated thatVerticillium alboatrum was present in potato fields in the irrigated coastal valleys of central Peru. No further studies were carried out in Peru until those in the late 1970’s initiated at The International Potato Center. So far, Verticillium wilt of potatoes has been observed in several valleys of the coast as well as in highland valleys at elevations up to 3400 m. Although the frequency of isolation ofVerticillium sp. has been much greater in the highlands, the disease is of less consequence there because of low temperatures during the growing season. The most common symptom observed in the field is early senescence, including yellowing and stunting of the foliage. Typical “wilt” symptoms are rarely found. Both in the coastal valleys and in the highlands, onlyVerticillium dahliae has been identified. Although no studies on losses and the economic importance of Verticillium wilt of potatoes have been done, it is clear that the two most important crops used in rotation in coastal valleys of central Peru, potato and cotton, are severely affected.  相似文献   

5.
Viable propagules ofVerticillium albo-atrum were recovered from roots and stems of naturally infected weeds and sunflower cultivars before or after burial in the field and from stems and roots of certain non-infested crops after burial in the field for seven months.Verticillium dahliae was recovered less frequently, then only from the roots ofSolanum nigrum (black nightshade). Survival of these pathogens after burial for 7 months varied considerably, ranging from 38 to 58% in sunflower stems and from 19 to 235% in roots of weeds. In stems of weeds and crops which did not contain propagules ofV. albo-atrum before burial, the fungus was recovered consistently after burial, indicating that saphophytic colonization probably occurred during interment.  相似文献   

6.
Management practices for the suppression of Verticillium wilt of Russet Burbank potato include sanitation, use of optimum sprinkler-irrigation practices, soil solarization, and an adequate soil fertility program. Among all cultural factors considered, nitrogen (N) deficiency in potato tissue was most commonly associated with the severity of Verticiilium wilt in Russet Burbank potato. Field studies have shown that increased N availability suppresses Verticiilium incidence on cv Russet Burbank while having no effect on cv Norgold Russet. Studies from both greenhouse and field show that the colonization ofV dahliae in potato stem tissue is suppressed in cv Russet Burbank when the availability of Phosphorous (P) is increased to the optimum. Following one season of cropping with Russet Burbank potato, the treatment providing the optimal N availability also suppressed the increases ofV dahliae populations in the soil during the following year of consecutive cropping. Similarly, after two seasons of continuous potato cropping, treatments with optimal P had lower soil populations ofV. dahliae in soil. Results show the suppression of Verticiilium wilt with optimal fertility. Verticiilium wilt [caused by eitherVerticiilium dahliae Kleb, (microsclerotial form) orVerticiilium albo- atrum Reinke and Berthold (dark mycelial form)] is one of the most severe diseases of potato in the United States. Potato yields, tuber size, and specific gravity may be substantially reduced by this disease, depending on severity, time of occurrence, and growing season. In Idaho and other arid growing regions of the United States, Verticiilium wilt is caused byVerticillium dahliae Kleb. Idaho field studies involving cropping practices, soil fumigation treatments, solarization, and Verticillium-resistant potato clones all support the importance ofV dahliae upon potato yield. Data from these studies show that yield losses due toV. dahliae commonly approximate 5 to 12 metric T/ha (5, 6). Table 1 illustrates the effect of several cropping practices upon relativeV dahliae populations in soil with the effects upon both disease severity and potato yield. With continuous cropping of Russet Burbank,V dahliae populations in soil increased, and yields were reduced by 18 to 19 metric Tlha when compared to locations that had been allowed to remain either weed-free and fallow or where corn had been previously cropped. It was estimated thatV dahliae was influencing yield by about 10 to 12 metric Tlha based on a regression analysis of yield as a function ofV dahliae populations in soil. Similarly, whenV dahliae was suppressed by fumigation treatments involving mixtures of dichloropropene and picfume, yields more significantly increased by 6.5 to 12 metric T/ha (5). Although many factors may influence yields, clones with higher Verticillium resistance than Russet Burbank generally out-yield Russet Burbank. Figure 1 demonstrates the effect of solarization (a technique involving the laying of clear plastic on soil for the purpose of elevating soil temperatures to killV dahliae). When Verticillium was controlled in this manner (lo), there was no significant yield difference between the Russet Burbank potato and the highly resistant A68113-4 clone. The A68113-4 clone grown in non-solarized soil out-yielded the Russet Burbank in non-solarized soil by 31 percent while the solarization treatment significantly increased yield for the Russet Burbank and A68113-4 clones by 46 and 18 percent, respectively. These yield responses were observed in a field where inoculum levels ofV dahliae were relatively low (2.10 cfulgrn of soil). With these losses, the need to develop control procedures is great and to achieve this control the need also exists to accurately evaluate the effect of treatments uponV dahliae. Our Idaho studies have routinely utilized such a procedure (9) in combination with comparisons of both disease severity and yield.  相似文献   

7.
Nine soils collected in New Jersey and Maryland were infested with microsclerotia ofVerticillium dahliae and planted with potato cv. Superior for five successive crops in the greenhouse to induce suppressiveness to Verticillium wilt. Potatoes were harvested 9–12 wk after planting and disease incidence was determined by recovery ofV. dahliae from stem segments on selective medium. Six of the nine soils had high disease incidence during the first cropping and remained conducive to Verticillium wilt throughout the experiment. Disease incidence increased in soils 3 and 9 over time, while disease incidence decreased nonlinearly in soil 1. Viability of microsclerotia buried in soil 9 for 4 wks was reduced compared to the viability of microsclerotia buried in soils 1, 3, and 6. Addition of 50 or 100 g hydrated lime/10 kg soil to soil 9 eliminated its suppressiveness to Verticillium wilt. Repeated cropping of the same soil can induce suppressiveness to Verticillium wilt. Soils exhibiting induced suppressiveness may be a source of new antagonists againstV. dahliae.  相似文献   

8.
The incidence of soilborne fungal pathogens of potatoes and root lesion nematodes were determined in preceding crops of clovers, annual ryegrass, and winter wheat, in the succeeding potato crops and soils and severity of associated potato diseases recorded in two, 2-yr field trials. Differences were not evident in yearly recovery ofRhizoctonia solani, Verticil-Hum dahliae, V. alboatrum, andColletotrichum coccodes from previous crops and soils. However, overall trends were present with a higher recovery ofV. dahliae from wheat and a greater recovery ofR. solani from clover than either wheat or ryegrass. A previous crop of ryegrass resulted in lower levels ofR. solani in succeeding potato soils in both trials and after clover in one trial at the pre-harvest sampling time. In trial 1 greater numbers ofPratylenchus penetrans were recovered from clover soils than from soils of ryegrass or wheat. One instance of lower nematode recovery in potato soils following ryegrass occurred but no differences in their recovery from potato roots were observed. Potato wilt incidence was lowest after clovers. Stem rot severity was positively correlated with recovery ofR. solani andV. dahliae from potato stems and roots and negatively correlated withV. alboatrum from potato stems only. Nematode populations were not related to potato disease symptom severity ratings. Results reported herein indicate that only a portion of soilborne diseases of potatoes on Prince Edward Island may be related to previous crops produced in the 3-yr rotation selected for this study.  相似文献   

9.
K. Scholte 《Potato Research》1989,32(3):377-385
Summary The incidence ofVerticillium dahliae andColletotrichum coccodes was studied in a crop rotation experiment on sandy soil from 1983 to 1986. Early in the growing season the percentage of stems infected byV. dahliae increased with increasing cropping frequency of potato, depending on the cropping sequence, and decreased with the application of granular nematicides. However, later in the growing season the initial differences decreased.C. coccodes infections of stems were not affected by these factors. The percentage of plants infected byV. dahliae closely correlated with the percentage of plants with wilt symptoms but this relationship was absent forC. coccodes. Early in the growing season, the more vigorous the plants, the greater the level ofV. dahliae infection. Soil infestation withV. dahliae increased with increasing cropping frequency of potato but was not affected by annual applications of granular nematicides.  相似文献   

10.
Teff (Eragrostis tef) is a fine stemmed annual grass and gluten free small grain that is of interest as a forage, cover, or a rotation crop. Little is known about the susceptibility of teff to many diseases. Teff could be grown in rotation with potato in the northwestern United States provided teff cultivation is economical and does not increase soil populations for pathogens affecting rotation crops such as Verticillium dahliae. Verticillium dahliae infects a wide range of dicotyledonous plants, making it one of the most important fungal pathogens of crop plants in North America, including potato. The objective of this study was to quantify the susceptibility of teff to eight V. dahliae isolates and compare the susceptibility of teff to eggplant. Teff was confirmed as a host for V. dahliae, as indicated by the presence of microsclerotia in teff stems and roots after artificial inoculation in two years of greenhouse studies. The number of microsclerotia produced in teff did not differ between mint and potato pathotypes of V. dahliae. No V. dahliae isolate produced significantly greater numbers of microsclerotia than any of the seven other isolates tested in a two-year study. Microsclerotia production of V. dahliae in teff was consistently less than in susceptible eggplant cv. Night shadow in both greenhouse experiments (P?<?0.02). It is unlikely that teff infected by V. dahliae will proliferate microsclerotia of mint or potato-aggressive pathotypes, especially when compared to susceptible eggplant cultivars.  相似文献   

11.
A three-year field study was conducted during 1984–1986 to determine the interactive effects of nitrogen and phosphorus fertilization on Verticillium wilt, and yield and quality of continuously-cropped Russet Burbank potato. The experiment was conducted on a calcareous, silt loam soil with low initial levels of NO3-N (0.9 mg/kg), P (3.5 mg/kg), andVerticillium dahliae (9 cfu/g of soil). The experimental design consisted of a factorial combination of three N treatments (unfertilized check, preplant N or split N) and three P treatments (0,120, or 240 kg P/ha) applied to the same plots during the three-year study. Nitrogen was applied at 0 or 300 kg N/ha in 1984 and 0 or 240 kg N/ha in 1985 and 1986. By the spring of 1986, soil P concentrations for the 0,120, and 240 kg P/ha treatments had increased to 7, 25, and 50 mg/kg and no additional P was applied. In addition to suppressing Verticillium wilt by as much as 95%, N & P treatments also reduced the rate of increase of soilborne inoculum ofV. dahliae. After one season of cropping, the N treatment providing the most efficient N fertilization (300 kg N/ha, split-application) resulted in significantly (p=0.01) lowerV. dahliae counts in soil than the other N treatments. After two seasons of continuous cropping, applying 120 to 240 kg P/ha produced lower populations ofV. dahliae in soil compared to the treatment with no added P. Generally, as N and P treatments approached the highest levels, both wilt incidence andV. dahliae colonization values were reduced. There was a progressive reduction in total yield in nearly all treatments during each year of continuous cropping. Reduction of yield and increased Verticillium wilt incidence was generally greater with N or P deficient plants than with optimally fertilized plants. The highest total and U.S. No. 1 yields and least Verticillium wilt were obtained each year by applying split N and 240 kg P/ha. Results show that optimal N and P can minimize both Verticillium wilt and yield losses that normally occur with intensive potato cropping.  相似文献   

12.
Summary An extracellular protein-lipopolysaccaride antigen (PLP) was purified from culture fluids ofVerticillium dahliae. Antiserum produced in rabbits to the PLP detected the antigen in homogenates of tubers, stems and leaves ofV. dahliae-infected potato plants but not in extracts of healthy potato tissue or extracts of potato plants infected by other fungal pathogens. The antigen was not detectable in extracts of potato isolates ofV. tricorpus, V. nigrescens andV. nubilum or various other fungi. The antigen was shown to be different from cross-reactive antigens detected by antisera to mycelial antigens. When used as a tool for specific diagnosis ofV. dahliae infection in potato, antiserum to PLP was more reliable than that prepared against fungal body antigens. Publication of the Agricultural Research Organization No 245-E.  相似文献   

13.
A survey of New Brunswick potato fields with symptoms of early dying was conducted in September 1990 and 1991. Root lesion nematodes (Pratylenchus spp.) were detected in 43 of 46 fields in 1990 and in 37 of 43 fields in 1991. In 1990 and 1991 populations averaged 910 and 410 nematodes g-1 dry root and 1030 and 720 nematodes kg1 dry soil, respectively. Previous crops did not affect nematode populations, except in one comparison where the number of nematodes in potato roots in 1990 was higher when the previous crop was cereal than in 1991 when the previous crop was peas.Pratylenchus crenatus was more prevalent thanP. penetrans. Root-knot nematodes(Meloidogyne hapla) were detected at a few sites at low population levels only in 1991. In 1991,Verticillium albo-atrum was detected in all 37 potato fields where fungal analyses were conducted, and the average population was 350 propagules g1 dry soil. Soil populations ofV. alboatrum did not differ in fields in which either Russet Burbank or Shepody were being grown, nor did previous crops have any effect.Verticillium dahliae was detected in 17 fields at very low populations, usually below 1 propagule g1 dry soil. There were no significant correlations (P<0.05) between populations of P.crenatus orP. penetrans in soil or roots and populations ofV. albo-atrum orV. dahliae propagules in soil.  相似文献   

14.
Samples of tubers collected from commercial potato seed lots produced across North America were assayed forVerticillium dahliae. V. dahliae was successfully isolated from 65 of 224 seed lots tested, a successful isolation rate of nearly 30%. Vegetative compatibility of the isolates was assessed through complementation tests using nitrate non-utilizing mutants. AllV. dahliae isolates belonged to vegetative compatibility group (VCG) 4. Of the 162 isolates recovered, 64% belonged to VCG 4A, 33% to VCG 4B, and 3% to VCG 4AB. All 39 of the isolates tested in the greenhouse on potato cv. Superior were pathogenic to potato. Disease symptoms developed earlier, were more severe, and plants died earlier when inoculated with VCG 4A compared to 4B isolates. As a group, AUSPC values were significantly higher (p=0.05) for VCG 4A than for 4B isolates. These data suggest that (1) commercial certified seed tubers from diverse locations are commonly infected withV. dahliae and thus may serve as primary sources of the pathogen; (2) potato isolates ofV. dahliae in North America belong to VCG 4A and 4B and these strains are widely distributed via seed tubers; and (3) VCG 4A and 4B are distinct pathotypes ofV. dahliae that vary in their aggressiveness to potato.  相似文献   

15.
The effects of removing or flaming potato vines and soil fumigation on population density ofVerticillium dahliae in soil, severity of Verticillium wilt, and tuber yield were studied in a field near Alliston, Ontario, between 1993 and 1996. Vines were physically removed or flamed using a propane flamer in September just before harvest and soil was fumigated with metam-sodium (Vapam) at 550 L/ha in October after harvest. Vine removal had no effect on soil populations ofV. dahliae, area under the disease progress curve (AUDPC), or tuber yield. Flaming once (1993), twice (1993 and 1994), or three (1993,1994, and 1995) times reduced the soil population density ofV. dahliae, and flaming twice (1993 and 1994) reduced AUDPC compared to the nontreated control, but had no effect on tuber yield. Fumigation once (1993) or twice (1993 and 1994) reduced pathogen density in soil and AUDPC, but did not increase tuber yield. Fumigation once (1993) or twice (1993 and 1994), in combination with flaming twice (1993 and 1994), was equally and significantly effective in reducing both population density ofV. dahliae in soil and AUDPC values and in increasing tuber yield in 1995. Annual flaming of vines in combination with soil fumigation once (1993) or twice (1993 and 1994) in the fall improved the control of Verticillium wilt of potato and realised the greatest profits.  相似文献   

16.
Effect of nitrogen and phosphorus fertilization rates on the incidence of Verticillium wilt caused byVerticillium albo-atrum orV. dahliae and tuber yield in potato cv Yukon Gold was evaluated in field plots. In three years of study,V. albo-atrum caused a higher incidence of wilt symptoms (74%–100%) thanV. dahliae (5%–81%) resulting in lower marketable tuber yield. In plots infested withV. albo-atrum, tuber yield ranged from 12.3-22.8 T ha1 compared to 18.1– 31.5 T ha-1 in plots infested withV. dahliae. Application of nitrogen above (280 kg ha-1) or below (70 kg ha-1) the recommended rate of 140 kg ha-1 at planting did not suppress foliar symptoms or protect against yield loss caused by eitherV. albo-atrum orV. dahliae. Similarly, phosphorus rate adjustments did not consistently affect disease incidence regardless of the pathogen. Management of nitrogen or phosphorus fertility rates at planting does not appear to be a viable approach for suppressing Verticillium wilt in the determinant, wiltsusceptible cultivar Yukon Gold.  相似文献   

17.
A procedure was developed and tested to evaluate potato germ plasm for relative rates of vascular colonization byVerticillium dahliae. The number of colony forming units (CFU) per ml of plant sap, extracted from stems and plated on a medium, was used to assess vascular colonization from plants grown for 100 days in a field plot infested withV. dahliae. Sap extracted from main stem tissue at the ground line gave more CFU ofV. dahliae than tissue at the mid and apical region of the main stem. Aliquots of 0.05, 0.1 and 0.2 ml of plant sap were equally efficient in measuring vascular colonization. Thirty-three clones were evaluated for amount of vascular colonization in field and greenhouse tests with the former giving the best results. Cultivars and clones resistant to Verticillium wilt in Idaho, Maine, Minnesota, North Dakota and New York each had a low index of wilt and a low number of CFU/ml ofV. dahliae in plant sap in these tests. Clones intermediate in both wilt index and vascular colonization as well as selections with a high wilt index and extensive vascular colonization were found using the fresh sap assay method. Correlation values of, r = 0.92, r = 0.91 and r = 0.86 were observed between CFU/ml and percentage of foliar wilt for 11 clones/cultivars in 1986, 1987 and 1988, respectively. The correlation between CFU/ml and both moisture and temperature for the cultivars Kennebec and Russet Burbank, during a 6-yr period was r = 0.83.  相似文献   

18.
Verticillium wilt, caused by the soil-borne fungus Verticillium dahliae, is one of the most important diseases of potato in North America. Soil incorporation of alfalfa residues prior to planting potato could be a nonchemical Verticillium wilt management tactic by reducing the number of viable microsclerotia in field soil. Verticillium dahliae microsclerotia were quantified in field soils where organic material from alfalfa was incorporated, and numbers of microsclerotia were compared to fields where alfalfa residue was not incorporated. In addition, bacterial metagenomics was utilized to characterize soils where organic material from alfalfa was or was not incorporated to determine if alfalfa residue incorporation facilitates the formation of soils that suppress or kill V. dahliae microsclerotia. The number of V. dahliae microsclerotia in soil was greater (P = 0.0003) in fields where crop residue was incorporated than fields without incorporation when chloropicrin was used as a fumigant. Conversely, the number of V. dahliae microsclerotia observed in potato plants did not differ (P = 0.4020) between fields where residues were or were not incorporated if chloropicrin was used. Alfalfa residue incorporation did not significantly alter the soil bacterial metagenome compared to fields not subject to residue incorporation in both years of study. Despite these conclusions, the method can be employed to analyze the effect of grower practices with the intent of linking a field practice to increasing soil bacterial diversity and decreasing Verticillium wilt severity on potato.  相似文献   

19.
The effect of plowing and deep-rip tillage, in combination with chemigation or shank injection of metam sodium, onVerticillium dahliae populations and disease development was assessed in two fields with differing soil types and potato rotations. Soil samples were collected on a geo-referenced basis at depths of 0 to 10 cm and 10 to 20 cm before tillage, after tillage, after chemical application, and before planting and assayed for the presence of the pathogen. Propagules ofV. dahliae were detected at 140 of 141 sites sampled prior to tillage. Most (74.4% in heavy, sandy loam; 63.1% in light, loamy sand) were concentrated in upper 10 cm of the soil profile. Plowing redistributed inoculum vertically while deep-rip tillage did not. In the non-chemical treated areas of both fields, theVerticillium population reached a maximum between 25 July and 8 August before declining to near pre-tillage levels. Overall, the population generally was lower in the field with heavy soil, higher organic matter content, and a 3-year crop rotation. Metam sodium appeared to be most effective when shank injected, as the levels of inoculum in both fields declined by 60% to 80% following this application method. Chemigation was ineffective in the lighter soil, but the inoculum density in the deep-rip tillage area of the field with the heavier soil declined by nearly 20% in the upper and 60% in the lower strata following this treatment. The number ofV. dahliae propagules at the 10- to 20-cm depth in the plowed area of the same field was reduced by 25% following chemigation, but remained unchanged in the upper strata. Wilt was reduced in both fields by as much as 50% with shank injection of metam sodium with concomitant increases in total yield, marketable yield and gross income. Increases in total yield were significant (P<0.05) for the main effect of chemical, in the plowed area of the field with the heavier soil type following shank injection. These data suggest that growers might benefit from altering their tillage and chemical application practices as part of an integrated approach to managing Verticillium wilt.  相似文献   

20.
Summary The interaction betweenErwinia carotovora subsp.atroseptica (Eca) andVerticillium dahliae and its effect on symptom development in potato cultivars showing different degrees of resistance to them was examined over two seasons in irrigated fields in a hot, dry climate. Four cultivars were used: Cara, highly resistant to blackleg and tolerant to Verticillium wilt; Pentland Crown also resistant to blackleg but susceptible to Verticillium wilt; Désirée, moderately susceptible to blackleg but tolerant to Verticillium wilt; and Maris Bard, susceptible to both diseases. Seed tubers were inoculated with Eca immediately after harvest in Scotland, to simulate natural infection, and then planted in Israel in soil naturally infested byV. dahliae. In Verticillium tolerant cultivars, Eca infection increased the rate of colonization byV. dahliae and significantly enhanced symptom development, as assessed by suppression of plant height and wilt symptoms. This report is a portion of the Ph.D. thesis of the senior author in Ben-Gurion University of the Negev.  相似文献   

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