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1.
两株酵母菌对番茄采后病害的防治效果   总被引：1，自引：0，他引：1  
拮抗菌L-1-6是从辣椒果实表面附生微生物中分离筛选出的对番茄早疫病(Alternaria solani)有拮抗作用的酵母菌;拮抗菌H-2是从李果实表面附生微生物中分离筛选出的对番茄根霉果腐病(Rhizopus nigricans)有拮抗作用的酵母菌。用浓度为1.0×106cfu·mL-1的拮抗菌悬浮液浸泡绿熟期海粉901果实2min,常温贮藏20d,结果表明,拮抗菌L-1-6和H-2悬浮液分别处理番茄果实均能延迟病害发生,分别降低腐烂率40.0%和18.3%,降低失水率0.56%和0.53%,降低总损耗43.2%和21.4%。等体积混合浓度为1.0×106cfu·mL-1的拮抗菌L-1-6+H-2悬浮液处理果实,表现为增加腐烂的发生。用两种拮抗菌悬浮液分别处理番茄果实对果实营养成分含量和感官品质没有不良影响。  相似文献   

2.
Strawberries (Fragaria × ananassa Duch.) were coated either with chitosan (1, 1.5, and 2% solution, w/v) or aloe vera (AV) gel and the coatings were air dried. Coated strawberries were put in a polypropylene box and stored in refrigerator (6 ± 1°C and 50 ± 5% relative humidity. The success of coating in retaining the postharvest quality of the strawberries was evaluated by determining respiration rate, firmness, weight loss, external colour change, ascorbic acid content, total soluble solids, acidity, pH, microbial decay and sensory quality. The incidence of microbial rot started on day-6 in uncoated and 1% chitosan coated strawberries. Strawberries coated with 1.5 and 2% chitosan were affected by microbial decay on day-9 of storage. On the other hand, rot incidence was initiated in AV gel coated strawberries on day-15 of storage. Aloe vera gel or chitosan coating reduced respiration rate, weight loss, and microbial decay and preserved firmness, ascorbic acid content, and other quality parameters, thus delaying ripening and the progress of fruit decay due to senescence or microbial attack. Furthermore, AV gel delayed the changes in external colour and retained all other postharvest quality of strawberries compared to chitosan coated or uncoated ones throughout the storage.  相似文献   

3.
Summary

The effect of Pichia membranefaciens on reducing post-harvest anthracnose rot caused by Colletotrichum acutatum in loquat fruit was investigated. Fruit were treated with different suspensions of P. membranefaciens before challenge with 1.0 105 spores ml–1 of C. acutatum, then incubated at 20°C for 6 d. The results showed that 1 108 colony-forming units (CFU) ml–1 of washed cell suspensions of the yeast provided better control of anthracnose rot than yeast in culture broth at the same concentration. Treatment with autoclaved cell cultures or culture filtrates did not prevent the incidence of disease. The concentration of a washed cell suspension of P. membranefaciens had a significant effect on the efficacy of controlling anthracnose rot in loquat fruit. At a concentration range from 1 106 to 1 109 CFU ml–1, the higher the concentration of the antagonist, the lower the incidence of the disease and the smaller the diameter of the lesions. In inoculated wounds of loquat fruit, populations of P. membranefaciens increased approx. 34-fold after 6 d of incubation at 20°C.Washed cell suspensions of P. membranefaciens also significantly inhibited spore germination and germ-tube growth of C. acutatum, in vitro. These results suggest that P. membranefaciens has potential as a biocontrol agent to reduce post-harvest decay caused by C. acutatum in loquat fruit.  相似文献   

4.
Tomato (Solanum lycopersicum) is a valuable vegetable crop rich in health-protective carotenoids, but breeding improvements are limited by its narrow genetic diversity. New mutants with enhanced and deficient carotenoid content in a single genetic background of tomato cv. MicroTom were developed via chemical mutagenesis. Genetic and metabolic analyses showed that mutant DC260, which exhibited fruit color alteration from red to deep red interlaced with orange color, had significant (P < 0.05) increases of lycopene (up to 42.8%) and ß-carotene (up to 61.5%) compared with control plants. Pearson correlation analysis of M1 and M2 generations in DC260 revealed that fruit color alteration was significantly (P < 0.05) correlated with lycopene (coefficient = 0.55) and ß-carotene content (coefficient = 0.63). The fruit color alteration of DC260 was controlled by a single gene at a heterozygous locus. In contrast, mutant DC107 and DC624, which exhibited fruit color alteration from red to orange-yellow, was significantly (P < 0.05) carotenoid-deficient with up to 346.3-, 10.8-, and 185.2- fold reductions of lycopene, ß-carotene, and total carotenoids, respectively, compared with the control plants. Carotenoid deficiency in DC170 and DC624 was responsible for the fruit color alteration and was controlled by a dominant gene at a homozygous locus.  相似文献   

5.
采用孢子悬浮液接种法,鉴定了多毛番茄LYC4叶、茎、果实对灰霉病菌的抗性。结果表明:多毛番茄LYC4整株接种的病情指数为0,离体叶接种的发病率为0;离体茎接种的相对茎侵染率为0,相对茎腐扩展速率为0;离体果实接种的相对病斑扩展速率为5.7%。多毛番茄LYC4叶、茎、果实3个部位接种均表现高抗,是1份优秀的灰霉病抗源材料。  相似文献   

6.
河套灌区番茄脐腐病发生较重,主要原因是番茄开花坐果后不再灌水,不能满足番茄正常生长的水分需求;施肥以化肥为主,重施氮肥,少施磷钾肥,不施钙肥,导致植株缺钙;河套灌区土壤盐渍化较高,生产中存在连茬、耕作管理粗放等。防治番茄脐腐病应从水分均衡供应、垄沟种植、增施有机肥、减施化肥和叶面补钙等方面着手。  相似文献   

7.
In the present study we have developed an efficient and effective method of synthetic seed production and its two-way germination system of Vitex trifolia, for easy transport of the propagules and efficient utilization of its in vitro regeneration system. Nodal segments harvested from 8-week-old in vitro cultures were encapsulated in calcium alginate beads. Three percent (w/v) Na-alginate polymerized in 100 mmol/L CaCl2.2H2O for 30 min produced clear and uniform beads. Germination of encapsulated beads with shoot and roots was achieved on Murashige and Skoog (MS) medium augmented with 6-furfurylaminopurine (KN, 2.5 µmol/L) + α-naphthalene acetic acid (NAA, 1.0 µmol/L). For multiple shoot production, synseeds were incubated on 6-benzyladenine (BA, 5.0 µmol/L) + NAA (0.5 µmol/L) augmented MS medium followed by in vitro rooting on MS + indole-3-butyric acid (IBA, 1.0 µmol/L). The synseeds produced retained about 90% regeneration potential even after 4 weeks of storage at 4°C. Genetic stability of the regenerated plants was evaluated using 13 inter simple sequence repeats (ISSR) primers. The study thus provides an efficient system for production of synthetic seeds, their storage and subsequent conversion into genetically identical plants.  相似文献   

8.
Recent trends towards greater fresh market use of ‘Bartlett’ pears has increased the need to extend its storage life to prolong the packing and marketing season in the United States Pacific Northwest region. Sixteen and 38%, respectively, of control fruit developed senescence disorders following 5 and 6 months of storage at ?1.1°C. Commercial standard controlled atmosphere (CA) conditions (O2 at 1.5 kPa and CO2 < 1 kPa) or edible coating (Semperfresh?, SF) prevent the appearance of senescence disorders for 5 months, but 9% and 16% of fruit, respectively, developed senescence disorders after 6 months. The combination of CA+SF completely inhibited senescence disorders for 6 months. Treatment with CA and SF, alone or in combination, maintained high-storage quality and developed ripening capacity with characteristic melting texture during storage. Senescence disorders were inhibited for 6 months by 0.3 µL l?1 1-methylcyclopropene (1-MCP), alone or combination with CA or CA+SF. In part these pears developed ripening capacity after 6 months of storage. The combination of CA+SF+1-MCP maintained the highest storage quality with dark green colour and hard firmness, which might be associated and proportional with reductions in ethylene synthesis and respiration rate after long-term storage.  相似文献   

9.
Summary

Heirloom tomatoes (Solanum lycopersicum L.) such as ‘Cuore di Bue’ are highly appreciated by consumers for their outstanding quality and flavour. Nowadays, they are often grafted onto vigorous rootstocks in order to overcome several soil-borne diseases. The present study was conducted in 2007 and 2008 in Southern Italy. Plants of ‘Cuore di Bue’, an heirloom ‘oxheart’ tomato, were grown in a greenhouse either as non-grafted plants, or grafted onto two inter-specific (S. lycopersicum S. habrochaites) rootstocks (‘Beaufort F1’ and ‘Maxifort F1’) in order to evaluate their effects on vegetative growth, marketable yield, fruit quality, and the sensory properties of ‘Cuore di Bue’ tomato fruit. Growth analysis revealed that ‘Maxifort F1’ enhanced plant growth, particularly in terms of leaf area and leaf fresh weight. Grafted plants had higher leaf area ratios (by 13%) and higher leaf dry weight fractions (by 18%) compared to non-grafted ‘Cuore di Bue’ plants. Marketable yields increased by 20 – 25% in grafted plants in both years. However, total soluble solids (TSS) contents, titratable acidity (TA), and TSS/TA ratios were not significantly affected by grafting.Vitamin C contents decreased by 14 – 20% in both years in the fruit of plants grafted onto either rootstock. The sensory profiles of fruit were not modified by grafting, although taste panelists expressed a higher preference for purchasing fruit from plants grafted onto ‘Maxifort F1’. These results confirm that, when using the appropriate rootstock/scion combination, grafting can improve plant growth and the marketable yield of heirloom tomato fruit without reducing the sensory quality of the fruit or its biochemical parameters. However, grafting can reduce vitamin C contents and, thus, nutritional quality.  相似文献   

10.
Calcium (Ca) has been considered a key element in maintaining fruit quality and extending the storage period of several fruits. The main blueberry (interspecific hybrids of Vaccinium corymbosum x V. darrowii) market for the Southern Hemisphere production involves a long transport between harvest and consumption. Foliar Ca sprays during the pre-harvest constitutes a common practice for improving Ca concentration [Ca] in fruits and therefore in alleviating the loss of firmness, dehydration and rot decay during postharvest. In this study, two formulations of Ca; Ca-nitrate [Ca(NO3)2] and chelate Ca-oxide (Ca-gluconate; Ca-Glu) were sprayed four times during pre-harvest of blueberry cv. O’Neal. [Ca] in the fruits was followed after each application and fruit quality parameters were determined at harvest and during 28 days of cold storage. Both formulations were ineffective in increasing [Ca] in the berries (or leaves) after any of the four applications. In fruits, [Ca] decreased from 0.32% at 25 days after full bloom (DAFB) to 0.11% Ca at harvest time. Other minerals such as magnesium (Mg), potassium (K), or nitrogen (N) remained unaffected by the treatments. Data shows that foliar pre-harvest applications of Ca, regardless of formula, were ineffective at increasing [Ca] or improving the quality of the fruit.  相似文献   

11.
The research was conducted in the pummelo growing areas of Thailand. The results showed that the dropped fruits had a statistically smaller fruit size (7.25 cm) than the retained fruit (9.35 cm; P < 0.05) at 4 months after fruit set. Moreover, the dropped fruits had an abnormal fruit shape when compared to the retained fruits. Consideration of fruit and leaf carbohydrate concentrations showed that the retained fruits had statistically higher carbohydrate concentrations (262 mg g?1) than the dropped fruits (203 mg g?1; P < 0.05). Meanwhile, the dropped fruits had statistically lower leaf carbohydrate concentrations (55 mg g?1) than those in the retained fruits (85 mg g?1; P < 0.05). The retained fruits also had statistically higher N, P, and K concentrations (1.6%, 1.1%, and 1.3%, respectively) than the dropped fruits (1.1%, 0.5%, and 1.0%, respectively; P < 0.05). Therefore, the causes of early fruit drop in pummelo could be insufficient carbohydrate and plant nutrients supplied for fruit growth and development.  相似文献   

12.
We stored plug seedlings of tomato (Solanum lycopersicum L. cv. Momotaro) under limited fertilisation (LF) for 12 weeks, and evaluated growth and development during storage and after planting. Seedlings in the LF treatment were grown in substrate containing starter fertiliser and irrigated with water. Controls (CT) were irrigated with nutrient solution. Stem growth, leaf growth, and biomass accumulation slowed or ceased during storage of LF seedlings, while CT seedlings showed increases in these parameters. At 2 weeks after planting, the stem length of LF seedlings was shorter than that of CT seedlings, but the two seedling types had similar numbers of leaves. At harvest, LF and CT plants had similar numbers of leaves under the fruit trusses, but the trusses formed at lower heights in LF than in CT seedlings. LF and CT plants produced similar numbers of fruits and similar yields from the first to third trusses. These results indicate that tomato plug seedlings can be stored for more than 2 months under LF.  相似文献   

13.
Nicht-destruktive Bestimmung der Beduftung bzw. des Duftfilmes bei Pflaumen   总被引：1，自引：1，他引：0  
During their ontogeny, a range of fruits develop a semi-transparent white coating on their surface, designated as wax bloom. This wax bloom was examined non-destructively on plums of different colour i.e. blue mature European plum, yellow mirabelle and greengage using a colourimeter and an industrial luster sensor at 15 mm distance from the fruit surface; the results are displayed as ‘luster levels’. European plums contained 175–205 µg epicuticular wax cm?2 fruit surface with linoleic and ursolic acid as predominant fatty acids. The microscopic examination identified the epicuticular fine structure of the fruit surface as platelets viz with natural wax. Polishing with a soft tissue disturbed this fine structure and doubled the luster levels relative to the natural surface from 120–170 to 300–500 arbitrary units. This is related to wax re-distribution and restructuring rather than loss of epicuticular wax. Polishing increased the a value marginally from 2.35 to 2.85, whereas the b values almost tripled from ? 14 to ? 5, representing a more intense blue impression after polishing. This luster or glossiness measurement enables a clear identification of the natural wax coating versus a polished fruit surface irrespective of the respective fruit colour, i.e. blue plum, yellow mirabelle or greengage and hence seems more suitable than colour measurement. It hence seems plausible that fruit with a similar natural wax bloom like grape berry or blueberry etc. might respond similarly and could be used as a hand-hand device or on a sorting line for fast, online and affordable detection of the glossiness of a commodity.  相似文献   

14.
Summary

The compound benzo-(1, 2, 3)-thiodiazole-7-carbothioic acid S-methyl ester (BTH) effectively induces a systemic acquired resistance (SAR) response in plants and increases plant resistance to disease. To learn how BTH may affect post-harvest quality and disease resistance in peach, (Prunus persica L. cv. ‘Jiubao’) peach fruit were treated with 200 mg l–1 BTH for 5 min immediately after harvest, then incubated for 60 h at 20°C, 85–95% RH. Sixty h after treatment, fruit were inoculated with spore suspensions of Penicillium expansum at 1.2 104 CFU ml–1. The incidence of disease and lesion areas on peach fruit were significantly reduced by BTH-treatment (P < 0.05). In addition, chitinase and -1,3-glucanase activities in peach fruit were significantly enhanced by BTH-treatment (P < 0.05). Moreover, the total levels of phenolic compounds and chlorogenic acid, and the lignin content of BTH-treated fruit were significant higher than in control fruit (P < 0.05). The effects of BTH on fruit quality were also analysed. Soluble solids content, soluble pectin, titratable acidity and ascorbic acid levels in peach fruit were not significantly affected by treatment with BTH. Our results suggest that the application of BTH may control post-harvest diseases in peach fruit during commercial production.  相似文献   

15.
This study reports on in vitro regeneration of Abutilon indicum plantlets through callus mediated organogenesis. The leaf explants implanted on Murashige and Skoogs (MS) medium supplemented with 4.52 µM 2, 4-Dicholorophenoxy acetic acid (2,4-D) and 8.88 µM 6 Benzyladenine (BA) showed highest response (70.3%) for callus proliferation, but these callus did not showed any morphogenetic differentiation on the same medium even after 12 weeks. Whereas, subsequent sub-culture of this green proliferated callus on MS medium added with 2.68µM α-Napthalene acetic acid (NAA), 8.88µM BA and 543 µM Adenine sulphate showed the highest frequency (62.2%) of multiple shoot-buds production and also elongation of shoots. Well developed shoots were efficiently rooted in vitro on half strength MS medium supplemented with 7.38 µM Indole-3-butyric acid (IBA). Seventy per cent of in vitro regenerated plantlets were successfully established in garden and were morphologically alike to the donor plants. The genetic homogeneity of these in vitro regenerated plantlets was also affirmed by inter simple sequence repeat (ISSR) analysis using eight ISSR primers. This standardised in vitro organogenesis protocol supplements a good platform for the conservation of A. indicum germplasms and also caters for the needs of the herbal industry.  相似文献   

16.
The effects of two oxylipins – jasmonic acid (JA) and 9, 10-ketol-octadecadienoic acid (KODA) – on ethylene metabolism and ethylene receptor genes were examined in peach fruit (Prunus persica L.) harvested at 88 days after full bloom (DAFB), in the pre-climacteric stage, and at 102 DAFB, at the beginning of the climacteric stage. Immediate post-harvest applications of either n-propyl dihydrojasmonate (PDJ) (a JA analog) or KODA stimulated ethylene production and 1-aminocyclopropane-1-carboxylate (ACC) concentrations in fruit that were harvested at 88 and 102 DAFB. KODA application significantly increased levels of ACC oxidase (PpACO1) expression at 5 and 8 days after treatment (DAT) in 88-DAFB-harvested fruit, and at 1 DAT in 102-DAFB-harvested fruit. Expression levels of ethylene response factors (PpERS1) were not influenced by either PDJ or KODA applications at 88 or 102 DAFB. In contrast, levels of ethylene-receptor-coding genes (PpETR1) expression in 88-DAFB KODA-treated fruit increased at 8 and 14 DAT. Expression levels of constitutive triple response (PpCTR1) in the untreated control fruit decreased with DAT in both 88- and 102-DAFB-harvested fruit.

These results suggest that certain oxylipins may promote ethylene production in peach fruit, and that PpETR1 and PpCTR1 may be associated with the autocatalytic system of ethylene production.  相似文献   


17.
ABSTRACT

Effect of 2,4-D and naphthalene acetic acid (NAA) on fruit drop reduction in pummelo cv. Thong Dee was investigated in the pummelo growing areas of Nakhon Pathom province, Thailand. Five similar sized and aged of pummelo trees were selected to set up the experiment. Ten mature branches with the same size from each pummelo tree were randomly selected around the canopy for 2,4-D (20 and 40 mg L?1), NAA (20 and 40 mg L?1) application and control. All treatments were applied to selected pummelo branches 2 times at full bloom and 2 months after fruit set. The results showed that 20 mg L?1 NAA (14.84%) and 40 mg L?1 NAA (12.26%) gave significantly higher percent of fruit retention at 6 months after fruit set. However, leaf total nonstructural carbohydrate concentration analysis showed that 40 mg L?1 2,4-D (104.86 mg g?1) and 20 mg L?1 2,4-D (96.55 mg g?1) gave significantly higher total nonstructural carbohydrate than those in control (78.44 mg g?1). For fruit quality, 40 mg L?1 2,4-D and 20 mg L?1 2,4-D gave the highest peel weight with 435.55 and 358.57 g, respectively, and 40 mg L?1 2,4-D gave the highest peel thickness with 20.25 mm, while 20 mg L?1 NAA gave statistically higher total soluble solid than those in 20 mg L?1 2,4-D and 40 mg L?1 2,4-D. Therefore, 20 mg L?1 of NAA sprayed 2 times at full bloom and 2 month after fruit set effectively reduced fruit drop and increased percentage of fruit retention in pummelo cv. Thong Dee.  相似文献   

18.
张海芳  焦立东  张宁宁  李超  王猛 《蔬菜》2021,(11):77-80
为筛选出较适宜京郊地区日光温室无土栽培的中果型优质番茄品种，于2019年引进6个中果型番茄品种进行无土栽培比较试验，综合比较各品种植物学性状、果实性状、产量及发病率等情况。结果表明：味多美2号产量最高，667 m2产量达6 447.67 kg，长势较旺，果实口感较好，且耐贮运，脐腐病发病率较低，较适宜在大兴地区进行冬春茬日光温室无土栽培。  相似文献   

19.
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20.
Two liquid culture systems, rotary shaker (RS) and temporary immersion system (TIS) for the propagation of Rosa rubiginosa and Rosa tomentosa were investigated and compared with solid medium culture. Shoot tips were grown on basic Murashige and Skoog medium with 20 mg dm?3 Fe EDDHA, 1 µM BA, 1.5 µM GA3 and 3% sucrose for six weeks. Of the different culture systems, liquid cultures resulted in better growth and development of roses. Both RS and TIS cultures improved biomass growth, multiplication rate, leaf blade area and plant height; however, RS reduced the shoot dry mass content. There were no changes in the content of chlorophyll a, b, a + b in plants developed in the tested systems. Use of a solid medium favoured the accumulation of phenolic compounds, soluble sugars, and carotenoids, but it was accompanied by browning and necrosis of shoots. Plants propagated in TIS were characterised by the high content of phenolic compounds and soluble sugars, but they had the highest multiplication rate. Use of TIS resulted in eight times the number of shoots for R. tomentosa and twice as many for R. rubiginosa compared to solid medium.  相似文献   

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