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1.
Tilapia is a warmwater fish with mild flavor. Nearly 8.6 million kg are produced domestically, and ≈22.7 million kg are imported. Corn gluten meal (60% protein fraction) is a product obtained from wet-milling of corn. Diets (36% protein) containing 36–44% corn gluten meal with different levels of lysine and fish meal were formulated and fed to tilapia in aquaria for 12 weeks. Weight gain (WG) of tilapia fed diets containing the highest level of lysine (7.4% protein) with 4% fish meal was equal to that of fish fed a commercial control diet. Diets with lower lysine resulted in lower WG. The feed conversion ratio (FCR) and protein efficiency ratio (PER) of tilapia fed experimental diets containing adequate levels of essential amino acids and fish meal were the same as for fish fed the commercial control diet (also containing fish meal). Fish fed diets containing lower lysine levels had less favorable FCR and PER. This study shows that corn gluten meal is utilized at high levels in tilapia diets, particularly if essential amino acids are provided in adequate amounts.  相似文献   

2.
配制蛋白质水平20%~50% 的7种等能半精制实验饲料,饲养平均初始体重为1.26±0.02 g 的7组3重复的刺鲃(Barbudes caldwell)幼鱼8周,研究刺鲃对饲料蛋白的需求量。结果显示,饲料蛋白水平对实验鱼的成活率、脏体比和肝体比等均无显著影响(P >0.05),而对鱼体终重有显著影响(P <0.05);鱼体增重和生长比速均随饲料蛋白水平从20%升高到35%而不断提高,但饲料蛋白水平进一步提高,则鱼体增重和生长比速均无显著差异(P >0.05)。饲料蛋白水平30%～50%的5个实验组的饲料效率无显著差异(P>0.05), 但均显著高于饲料蛋白为20%和25%的2个实验组(P <0.05)。蛋白质效率(PER)与饲料蛋白水平(x)呈负相关(PER= 3.006－0.03251x, R =0.9366)。饲料蛋白水平对鱼体的胴体水分、粗蛋白和粗灰分的含量无显著影响(P > 0.05);胴体脂肪含量随饲料蛋白水平(x)的升高而不断降低(L=8.2169－0.0458x,R =0.8551)。实验鱼肝胰脏蛋白酶活性则在组间无显著差异(P > 0.05)。肠蛋白酶和肝胰脏淀粉酶活性均随着饲料蛋白水平的升高而呈现先逐渐升高,且升高到一定程度后又呈逐渐下降的趋势。肠淀粉酶活性(IAA)与饲料蛋白水平(x)呈负相关关系(IAA = 84.625－0.9147x, R =0.8463,)。以鱼体增重为指标(y),与饲料蛋白水平(x)拟合折线模型进行回归分析,估算出饲料蛋白质适宜水平为占干饲料的34%。  相似文献   

3.
This study was designed to assess the effect of dietary soybean phospholipids on canthaxanthin transport by serum lipoproteins and canthaxanthin muscle deposition in trout. Three groups of 12 immature trout in triplicate with a mean body weight of 130 g were fed with three experimental diets containing (1) canthaxanthin plus lecithin plus fish oil, (2) canthaxanthin plus lecithin, and (3) canthaxanthin alone, for 12 days. The two major lipoprotein classes in rainbow trout are high-density lipoproteins, which transport principally carotenoids present in the serum, and low-density lipoproteins, which are responsible for the transport of cholesterol, both independently of the administered diet. In addition, very low density lipoproteins are responsible for triglyceride transport in serum. Nevertheless, the amount of canthaxanthin in the serum increased when carotenoid was associated with phospholipids plus fish oil. When canthaxanthin is transported by lecithin plus fish oil, the amount of phospholipids, cantaxanthin, and cholesterol deposited in muscle increased but not significantly. The highest apparent canthaxanthin digestibility coefficient was obtained when canthaxanthin was carried by lecithin plus fish oil. The administration of canthaxanthin carried by phospholipids improved its accumulation in the muscle of rainbow trout. This accumulation could be enhanced if the time of administration of canthaxanthin is increased.  相似文献   

4.
Fingerling rainbow trout were fed a diet fortified with 10 ppm Chlorowax 500C, a chlorinated paraffin, for up to 82 days. Chlorinated paraffin residues as high as 1.1 ppm (tissue basis) were found. Recoveries from fortified fish tissue were greater than 92%, and the method sensitivity (tissue basis) is at least 0.5 ppm. Ultraviolet irradiation of the sample extracts and microcoulometric gas chromatography minimized interferences. No gross toxicological effects were noted in the experimental fish, although their weight gain was less than that of the controls.  相似文献   

5.
Gossypol is an antifertilizing agent in males and females. However, gossypol and its metabolite, gossypolone, have also gained interest because of their anticarcinogenic activities. This paper examines for the first time both enantiomers of tissue gossypol and gossypolone in mature rainbow trout fed two diets containing low (15%) and high (60%) levels of cottonseed meal (CM) for 9 months. The gossypol concentration was highest in liver followed by kidney, intestine, testis, blood plasma, stomach, and muscle. Gossypol was detected in muscles of fish fed low- and high-CM diets (0.31 +/- 0.03 and 1.95 +/- 0.59 microg of total gossypol/g, wet basis, respectively). The (+)-gossypol enantiomer was predominantly retained in all tissues. The ratio of (-)- to total gossypol ranged from 30 to 44% in fish fed the high-CM diet and from 23 to 30% in fish fed the low-CM diet except for muscle tissue (44%). Higher gossypolone concentrations were found in intestine than in liver. Gossypolone, however, was not detected in blood plasma, muscle, and testis of fish fed the low-CM diet. The ratio of gossypolone to gossypol was highest in muscle (1.75), followed by intestine (1.59), stomach (1.50), kidney (0.43), liver (0.34), testis (0.28), and blood plasma (0.27). This study indicated that the retention of the (-)-gossypol enantiomer is dependent on dietary concentrations and that the oxidative conversion of gossypol to gossypolone occurs more actively in the digestive tract and muscle than in other tissues in rainbow trout.  相似文献   

6.
Arachidonic acid (AA) content, long-chain n-3 polyunsaturated fatty acid (PUFA) equivalent [LCE; calculated as 0.15 x linolenic acid (LA) + eicosapentaenoic acid (EPA) + docosahexaenoic acid (DHA)], and PUFA n-6/PUFA n-3 ratio were determined in meat [breast meat (BM), thigh meat (TM), and fillets (F), respectively] within four sets of chickens, five sets of turkeys, one set of common carp, and four sets of rainbow trout, fed either commercial diet or diets with manipulated PUFA n-3 and PUFA n-6 contents. AA content was within the range of 20 mg/100 g (F of rainbow trout fed the diet with linseed oil, LO) to 138 mg/100 g (TM of chickens fed restrictively the diet based on maize to the age of 90 days). AA content in BM of turkeys fed the diet with LO or fish oil (FO) did not differ (P > 0.05) from that of rainbow trout F. LCE was in the range of 16 mg/100 g (BM of turkeys fed a commercial feed mixture) to 681 mg/100 g (F of rainbow trout fed a commercial feed mixture). With regard to BM, only turkeys fed the diet with LO deposited more (P < 0.01) LCE (71 mg/100 g) as compared to all other poultry sets except turkeys fed the diet with FO (123 mg/100 g). Apart from all fish samples, also both BM and TM of turkeys fed the diet with either LO or FO met the recommended value of the PUFA n-6/PUFA n-3 ratio (<4). AA content in the tissue increased significantly (P < 0.001) with increasing dietary LA in both all chicken tissues and all turkey tissues, which is contrary to the suggested strong metabolic regulation of the AA formation. When all tissues within all animal species were taken as a one set, both AA percentage and EPA + DHA percentage in the tissue (Y, %) decreased (P < 0.001) with increasing fat content in the tissue (X, %), according to the equation Y = 4.7 - 0.54X (R (2) = 0.41) and Y = 6.0 - 0.33X (R (2) = 0.35), respectively. AA content in chicken BM, chicken TM, and turkey BM, respectively, decreased linearly (P < 0.01) with increasing live weight reached at the slaughter age.  相似文献   

7.
The distribution and potential bioaccumulation of dietary arsenic, cadmium, lead, mercury, and selenium in organs and tissues of rainbow trout (Oncorhyncus mykiss Walbaum, 1792), a major aquaculture species, was studied in relation to fish growth over a period of >3 years. Fish were reared under normal farming conditions, that is, fed a standard fish food and exposed to negligible levels of waterborne trace elements. The age-related variations in the content of each trace element in gills, kidney, liver, muscle, and skin were studied through nonparametric regression analysis. A buildup of all elements in all tissues and organs was observed, but due to dilution with growth, the concentrations did not increase, except in a few cases such as cadmium and mercury in liver and kidney. In muscle tissue, the concentrations of mercury, lead, and selenium did not alter significantly with growth, whereas cadmium increased but remained at exceedingly low levels. The concentration of arsenic in muscle tissue peaked at 14 months and then decreased in adult specimens. Arsenic speciation by high-performance liquid chromatography--inductively coupled plasma mass spectrometry revealed that arsenic in muscle was almost exclusively present in the form of nontoxic arsenobetaine. Application of a mercury mass balance model gave predicted concentrations in agreement with measured ones and showed that in farmed rainbow trout the ratio of mercury concentrations in feed and in fish is about 1:1. Therefore, rainbow trout does not approach the limits established for human consumption even when reared with feed at the maximum permitted levels. These findings highlight the low bioaccumulation potential of toxic trace elements such as cadmium, lead, and mercury in rainbow trout following dietary exposure. On the other hand, selenium concentrations in muscle (about 0.2 microg g (-1) of fresh weight) show that rainbow trout may be a good source of this essential element.  相似文献   

8.
Four-week-old male golden Syrian hamsters were fed diets containing cellulose (control, CC), cellulose + soy protein (CS), CS + vitamin E, (CSE), rice bran (RB), RB + vitamin E (RBE), oat bran (OB), and OB + vitamin E (OBE) for six weeks (n = 10/treatment). Diets contained (by weight) 10% total dietary fiber, 3% N, 20% fat, 0.5% cholesterol, and some diets had an additional 0.1% vitamin E. After six weeks, RB and OB diets resulted in significantly higher weight gain than the CC diet. Plasma low-density lipoprotein cholesterol (LDL-C) values and the LDL-C/high-density lipoprotein cholesterol ratio in hamsters fed CSE, RBE, OB, and OBE diets were significantly lower than in those fed CC diet. There were no significant differences in total plasma cholesterol values among the hamsters fed any of the diets. Liver cholesterol in animals fed OB and OBE diets was significantly lower than in all other groups. Foam cell areas in the inner bend of the aortic arch in animals fed all treatment diets were significantly reduced when compared with that in animals fed CC diet. The level of additional dietary vitamin E did not result in further significant reductions in foam cell area. The results of this study suggest that diets containing rice bran, oat bran, or soy protein significantly reduced the development of atherosclerosis in hypercholesterolemic hamsters.  相似文献   

9.
Fish oil replacement in aquaculture feeds results in major modifications to the fatty acid makeup of cultured fish. Therefore, in vivo fatty acid biosynthesis has been a topic of considerable research interest. Evidence suggests that pyridoxine (vitamin B(6)) plays a role in fatty acid metabolism, and in particular, the biosynthesis of LC-PUFA has been demonstrated in mammals. However, there is little information on the effects of dietary pyridoxine availability in fish fed diets lacking LC-PUFA. This study demonstrates a relationship between dietary pyridoxine supplementation and fatty acid metabolism in rainbow trout. In particular, the dietary pyridoxine level was shown to modulate and positively stimulate the activity of the fatty acid elongase and Δ-6 and Δ-5 desaturase enzymes, deduced by the whole-body fatty acid balance method. This activity was insufficient to compensate for a diet lacking in LC-PUFA but does highlight potential strategies to maximize this activity in cultured fish, especially when fish oil is replaced with vegetable oils.  相似文献   

10.
We examined the effects of low-dose fish oil ingestion on hepatic lipid accumulation caused after high cholesterol feeding in C57BL/6J mice. The mice were fed purified experimental diets consisting of 20 energy % (en%) safflower oil (SO or SO/CH), 2 en% fish oil + 18 en% safflower oil (2FO or 2FO/CH), or 5 en% fish oil + 15 en% safflower oil (5FO or 5FO/CH) with or without 2 weight % (wt %) cholesterol for 8 weeks. Hepatic triglyceride and total cholesterol contents were significantly lower in groups that were fed diets containing fish oil and cholesterol than in those that were fed safflower oil and cholesterol. The hepatic mRNA levels of fatty acid synthase (FAS) were lower in groups fed cholesterol or fish oil. Fatty acid oxidation-related hepatic gene expressions were higher in fish oil-fed groups. Fecal cholesterol excretion was higher in all cholesterol-fed groups; cholesterol excretion was high in groups fed fish oil and cholesterol. These results suggest that low-dose fish oil diets improve lipid metabolism by modifying the expression of lipid metabolism-related genes in the liver and increasing fecal cholesterol excretion.  相似文献   

11.
Fucoxanthin is a marine carotenoid found in edible brown seaweeds. We previously reported that dietary fucoxanthin attenuates the weight gain of white adipose tissue (WAT) of diabetic/obese KK- A(y) mice. In this study, to evaluate the antiobesity and antidiabetic effects of fucoxanthin and fish oil, we investigated the effect on the WAT weight, blood glucose, and insulin levels of KK- A(y) mice. Furthermore, the expression level of uncoupling protein 1 (UCP1) and adipokine mRNA in WAT were measured. After 4 weeks of feeding, 0.2% fucoxanthin in the diet markedly attenuated the gain of WAT weight in KK- A(y) mice with increasing UCP1 expression compared with the control mice. The WAT weight of the mice fed 0.1% fucoxanthin and 6.9% fish oil was also significantly lower than that of the mice fed fucoxanthin alone. In addition, 0.2% fucoxanthin markedly decreased the blood glucose and plasma insulin concentrations in KK- A(y) mice. The mice fed with the combination diet of 0.1% fucoxanthin and fish oil also showed improvements similar to that of 0.2% fucoxanthin. Leptin and tumor necrosis factor (TNFalpha) mRNA expression in WAT were significantly down-regulated by 0.2% fucoxanthin. These results suggest that dietary fucoxanthin decreases the blood glucose and plasma insulin concentration of KK- A(y) along with down-regulating TNFalpha mRNA. In addition, the combination of fucoxanthin and fish oil is more effective for attenuating the weight gain of WAT than feeding with fucoxanthin alone.  相似文献   

12.
Atlantic cod of initial mean weight approximately 220 g were fed a control diet and three diets in which fish meal (FM) was replaced with increasing levels of full-fat soybean meal (FFS) supplied at 12, 24, and 36% of dry diet, for 12 weeks. There were no significant differences in final weights, but the specific growth rate (SGR) was significantly higher in fish fed the control (FFS0) diet compared to fish fed the FFS12 and FFS36 diets, and the feed conversion ratio (FCR) was significantly lower in fish fed the FFS0 diet compared to the other three treatments. The fatty acid (FA) compositions of the cod muscle and liver were highly affected by dietary treatment, and linear relationships between dietary and tissue FA concentrations were shown for some of these. Moreover, selective utilization or accumulation in the tissues of specific FA was suggested by the results.  相似文献   

13.
The effects of fish oil and corn oil diets on diethylnitrosamine initiation/phenobarbital promotion of hepatic enzyme-altered foci in female Sprague-Dawley rats were investigated. Groups of 12 rats were initiated with diethylnitrosamine (15 mg/kg) at 24 h of age. After weaning, they received diets containing either 13.5% fish oil plus 1. 5% corn oil or 15% corn oil for 24 weeks. Rats fed fish oil had significantly greater liver weight, relative liver weight, spleen weight, and relative spleen weight than rats fed corn oil (p < 0.05). Hepatic phospholipid fatty-acid profile was significantly affected by the type of dietary lipid. The rats fed fish oil had significantly greater hepatic phospholipid 20:5 and 22:6 than rats fed corn oil; in contrast, the rats fed corn oil had significantly greater hepatic phospholipid 18:2 and 20:4 than rats fed fish oil (p < 0.05). Rats fed fish oil had significantly lower hepatic vitamin E and PGE(2) content but significantly greater hepatic lipid peroxidation than rats fed corn oil (p < 0.05). The hepatic levels of antioxidant enzymes (GSH reductase and GST) were significantly greater in rats fed fish oil than in rats fed corn oil (p < 0.05). Except for PGST-positive foci (foci area/tissue area), all the other foci parameters (GGT-positive foci area/tissue area, GGT-positive foci no./cm(2), GGT-positive foci no./cm(3), PGST-positive foci no. /cm(2), and PGST-positive foci no./cm(3)) measured in the fish oil group were 10-30% of those in the corn oil group (p < 0.05). Analyses of Pearson correlation coefficient revealed a positive correlation between hepatic GGT- or PGST-positive foci number (no. /cm(2)) and PGE(2) content (r = 0.66, P = 0.01; r = 0.56, P = 0.02, respectively) but a negative correlation between GGT- and PGST-positive foci (no./cm(2)) and lipid peroxidation (r = -0.8, P = 0.0006; r = -0.58, P = 0.01, respectively), GSH/(GSH + GSSG) ratio (r = -0.61, P = 0.05; r = -0.4, P = 0.14, respectively), GSH reductase (r = -0.75, P = 0.002; r = -0.53, P = 0.02, respectively), and GST activities (r = -0.65, P = 0.01; r = -0.44, P = 0.07, respectively). Similar correlation between foci number (no./cm(3)) and PGE(2), lipid peroxidation, GSH/(GSH + GSSG) ratio, GSH reductase, and GST activities were obtained. The results of this study show that dietary fish oil significantly inhibited hepatic enzyme-altered foci formation compared with corn oil in rats. These results suggest that the possible mechanisms involved in this process are the stimulation of hepatic detoxification system, changes in membrane composition, inhibition of PGE(2) synthesis, the enhancement of GSH-related antioxidant capacity, and the enhancement of lipid peroxidation by fish oil.  相似文献   

14.
This study aimed at investigating protein and lipid oxidation during frozen storage of rainbow trout. Rainbow trout fillets were stored for 13 months at -20, -30, or -80 degrees C, and samples were analyzed at regular intervals for lipid and protein oxidation markers. Lipid oxidation was followed by measuring lipid hydroperoxides (PV), as well as secondary oxidation products (volatiles) using dynamic headspace GC-MS. Free fatty acids (FFA) were measured as an estimation of lipolysis. Protein oxidation was followed using the spectrophotometric determination of protein carbonyls and immunoblotting. Significant oxidation was observed in samples stored at -20 degrees C, and at this temperature lipid and protein oxidation seemed to develop simultaneously. FFA, PV, and carbonyls increased significantly for the fish stored at -20 degrees C, whereas the fish stored at -30 and -80 degrees C did not show any increase in oxidation during the entire storage period when these methods were used. In contrast, the more sensitive GC-MS method used for measurement of the volatiles showed that the fish stored at -30 degrees C oxidized more quickly than those stored at -80 degrees C. Detection of protein oxidation using immunoblotting revealed that high molecular weight proteins were oxidized already at t = 0 and that no new protein oxidized during storage irrespective of the storage time and temperature. The results emphasize the need for the development of more sensitive and reliable methods to study protein oxidation in order to gain more explicit knowledge about the significance of protein oxidation for food quality and, especially, to correlate protein oxidation with physical and functional properties of foods.  相似文献   

15.
Genistein, the primary isoflavone in soybean, is one of the chemical components responsible for some of the off-flavors associated with soy-based foods. The potential effects of genistein on the sensory and chemical quality of fish muscle may affect the full utilization of soybean meal as an alternative protein in aquaculture diets. Fingerling trout fed commercial diets containing 0, 500, 1000, or 3000 ppm pure genistein were analyzed after 6 and 12 months of feeding. Genistein was extracted by enzymatic digestions in Tris buffer and quantified by high-performance liquid chromatography. Moisture, fat, protein, ash, and tristimulus color of the fillets were determined. The extent of lipid oxidation occurring in fillets harvested after 12 months of feeding was studied by measurements of thiobarbituric acid reactive substances (TBARS) after 4 and 8 days of refrigerated storage at 4 degrees C. Triangle tests were performed to determine if there were any detectable sensory differences. A dietary genistein content of 3000 ppm led to the deposition of approximately 5.4 pmol of genistein/mg of fillet. Triangle test panelists were unable to detect any significant (p < or = 0.05) differences between the fillets from trout fed the 0 and 3000 ppm genistein concentrations. Moisture, ash, and protein content were influenced by time of harvest, while color was unaffected. TBARS levels on days 4 and 8 were significantly (p < 0.05) higher in the fillets from the 0 ppm genistein level than in fillets from fish fed dietary genistein.  相似文献   

16.
本文以黑龙江省和北京市虹鳟养殖为例,应用生命周期评价方法,将虹鳟养殖生命周期划分为饵料生产、电力生产、化学品生产和养殖污染排放4个阶段,考虑了全球变暖潜势、能源消耗、酸化潜值和富营养化潜值4种环境影响类型,以获得1t养殖增重量为评价的功能单位,对虹鳟网箱养殖模式、工厂化流水养殖模式和工厂化循环水养殖模式的潜在环境影响进行了评价比较。结果表明,我国虹鳟养殖模式的环境影响从高到底依次是富营养化潜值、全球变暖潜势、酸化潜值和能源消耗;网箱养殖模式的环境影响指数分别为53.963、0.939、0.717和0.017,工厂化流水养殖模式的环境影响指数分别为35.213、4.827、2.896和0.049,工厂化循环水养殖模式的环境影响指数分别为7.404、5.545、3.305和0.055;富营养化潜值是虹鳟养殖的主要环境影响类型,其主要来自养殖污染排放。3种虹鳟养殖模式的环境影响综合指数分别为6.69、5.52和2.02,我国虹鳟养殖模式的环境性能从高到低依次为工厂化循环水养殖模式〉工厂化流水养殖模式〉网箱养殖模式。减少养殖污染排放、降低电能消耗和提高饵料利用率是提升我国虹鳟养殖模式环境友好性的关键。  相似文献   

17.
In Plastic Lake, Ontario, stocked rainbow trout (Salmo gairdneri) have failed to survive, one endemic fish species has become extinct and annual fish kills included up to five species, but especially pumpkinseeds (Lepomis gibbosus). The potential toxicity of Plastic Lake water was assessed by holding (hatchery) rainbow trout in the major inlet stream, the outlet, and in a portion of the outlet stream acidified to the pH of inlet No. 1. Stress on rainbow trout was assessed by measuring plasma and muscle concentrations of Na + Cl?, and K+, plus gill A1 concentration. Trout held in Plastic Lake inlet No. 1 showed a rapid loss of plasma Na+ from 138 to 85 meq.L?1and Cl? from 120 to 75 meq.L?1 within 24 hr. Gill A1 concentration increased from 20 to 105 μg.g?1 dry weight. Trout held in the outlet steam showed only slight loss of plasma Na+ and Cl? and no accumulation of Al on the gills. Trout held in the acidified outlet showed a significant loss of ions with plasma Na+ depressed from 140 to 115 meq.L?1 and plasma Cl? from 125 to 95 meq.L?1over 24 hr. Gill Al concentration increased from 18 to 30 μg.g?1 dry weight. The differences in stress response of rainbow trout held in the inlet and acidified outlet are likely due primarily to the difference in Al species concentrations in the two waters.  相似文献   

18.
Bayluscide [the ethanolamine salt of niclosamide (NIC)] is a registered piscicide used in combination with 3-(trifluoromethyl)-4-nitrophenol (TFM) to control sea lamprey populations in streams tributary to the Great Lakes. A high-performance liquid chromatography (HPLC) method was developed for the determination of NIC residues in muscle fillet tissues of fish exposed to NIC and TFM during sea lamprey control treatments. NIC was extracted from fortified channel catfish and rainbow trout fillet tissue with a series of acetone extractions and cleaned up on C(18) solid-phase extraction cartridges. NIC concentrations were determined by HPLC with detection at 360 and 335 nm for rainbow trout and catfish, respectively. Recovery of NIC from rainbow trout (n = 7) fortified at 0.04 microg/g was 77 +/- 6.5% and from channel catfish (n = 7) fortified at 0.02 microg/g was 113 +/- 11%. NIC detection limit was 0.0107 microg/g for rainbow trout and 0.0063 microg/g for catfish. Percent recovery of incurred radioactive residues by this method from catfish exposed to [(14)C]NIC was 89.3 +/- 4.1%. Percent recoveries of NIC from fortified storage stability tissue samples for rainbow trout (n = 3) analyzed at 5 and 7.5 month periods were 78 +/- 5.1 and 68 +/- 2.4%, respectively. Percent recoveries of NIC from fortified storage stability tissue samples for channel catfish (n = 3) analyzed at 5 and 7.5 month periods were 88 +/- 13 and 76 +/- 21%, respectively.  相似文献   

19.
The objective of the present study was the prevention of aflatoxicosis of growing rabbits fed aflatoxin (AF)-contaminated diet (833 microg of aflatoxins/kg) using 5% hydrogen peroxide (H2O2) and gamma-radiation at a dose level of 500 krad (5 kGy) and fed to growing rabbits. A total of 24 New Zealand white rabbits were divided into three groups. The experimental diets included AF-contaminated diet; AF-decontaminated diet, and AF-free diet (control). The obtained data showed significant reduction (p < 0.05) in live body weight and body weight gain of rabbits that fed on AF-contaminated diet as well as AF-decontaminated diet relative to control. There were no differences in feed consumption among the three groups; feed efficiency reduced significantly for AF-contaminated and AF-decontaminated groups. Mortality percentage was 25% for AF-contaminated and AF-decontaminated groups. Relative weight of the liver increased in animals fed AF-contaminated and AF-decontaminated diets, whereas the relative weight of kidneys decreased for both. There was no difference in total protein, but the levels of albumin and globulin were altered in rabbits receiving AF-contaminated diet. Serum enzymes (alanine amino transferase and aspartate amino transferase) activity increased significantly in rabbits that received AF-contaminated as well as AF-decontaminated diets. Histopathological examination revealed particularly alteration in liver and kidneys of rabbits fed AF-decontaminated diet. Results showed that the percentage of aflatoxin reduction ranged between 67 and 80% in boiled liver and between 79 and 90.5% in fried liver, whereas complete reduction in AF was found after boiling followed by frying. These findings indicate that the use of H2O2 and gamma-radiation for the destruction of aflatoxins in contaminated diet induces adverse effects in the animals.  相似文献   

20.
Net protein ratio data: AACC-ASTM collaborative study   总被引：1，自引：0，他引：1  
Seven- and 14-day net protein ratio (NPR) data were obtained from 7 laboratories for 6 protein sources: ANRC casein, lean beef, lactalbumin, textured vegetable protein, and peanut flour were fed as 10% protein (N X 6.25) in the test diet. Wheat flour, casein, and textured vegetable protein were fed as 6% protein (N X 6.25) in the test diet. Weighed dry ingredients for each diet were sent to each collaborator , who mixed the dry ingredients, then added specified amounts of corn oil and water and mixed each complete diet thoroughly. Rats were adapted for 0, 2, or 4 days, and then were fed the test diets for 28 days for protein efficiency ratio (PER) diets. The animal weight gain and feed consumption data obtained after 7 or 14 days of feeding were used to calculate NPR values. Analyses of data were done before [net protein ratio (NPR)] and after (R-NPR [relative-NPR]) adjustment of the data from each laboratory by its results for the reference protein casein. From the analysis of variance for NPR, significant (P less than 0.05) interactions were observed among laboratories, protein sources, and adaptation times of the animals (0, 2, or 4 days). Inter- and intralaboratory variability were decreased by use of 14-day values compared with 7-day values. Adjustment of the NPR data to R-NPR did not lower the intralaboratory variability but did lower the interlaboratory variability of the data. Increasing adaptation time did not consistently decrease interlaboratory or intralaboratory variability or decrease coefficients of variation (CV) of R-NPR values.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

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