共查询到19条相似文献，搜索用时 171 毫秒
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【目的】对狗舌草体积分数60%乙醇提取物的毒理学安全性进行评价。【方法】利用C57L-L小鼠,以生理盐水为阴性对照,环磷酰胺为阳性对照,进行狗舌草体积分数60%乙醇提取物的90 d长期毒性试验、30 d蓄积试验。并采用小鼠骨髓微核试验、小鼠生殖机能试验和小鼠胎儿外观畸形检查,对狗舌草体积分数60%乙醇提取物的长期毒性、蓄积毒性和特殊毒性进行评价。【结果】90 d长期毒性试验未见供试小鼠临床表现、体重和病理变化异常,30 d蓄积试验未见供试小鼠死亡,给药组与阴性对照组小鼠骨髓微核率无显著差异(P>0.05);与阴性对照组相比,用药后受孕鼠的受孕率、每窝活胎数、平均胎儿重、吸收胎儿数和死胎数均无明显变化(P>0.05),供试小鼠胎儿未见明显的外观畸形(P>0.05)。【结论】狗舌草体积分数60%乙醇提取物无长期毒性,无蓄积毒性,无致突变性和致畸胎性。 相似文献
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建立淋巴性白血病L1210细胞荷瘤DBA/2小鼠模型,从荷瘤鼠T/C及腹围测定、荷瘤鼠腹水中L1210细胞形态观察、荷瘤鼠白细胞象检查和骨髓象检查几方面,检查狗舌草60%乙醇提取物在体内对淋巴性白血病的作用效果。发现狗舌草60%乙醇提取物与单猪屎豆碱和槲皮素在体内抑制淋巴性白血病方面具有一致性。 相似文献
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试验以草绿菌株(Curvula rialunata)为病原，对9种常见禾本科草进行接种后，观测草的生长情况、症状出现时间、发病部位、病斑大小、病斑密度等指标，综合评价供试草的抗感性。结果说明，弯孢叶斑病菌都可以侵染供试草，使草生长速度降低，新生叶片形成幔，形成的叶片短而窄，节闻缩短。但是症状出现时间与严重程度不同，症状由强到弱的顺序为：剪股颖、狗牙根、多花黑麦草、鸭茅、高羊茅、牛鞭草、一年生早熟禾、多年生黑麦草、假俭草。供试禾本科草的抗感性：假俭草为R，多年生黑麦草、一年生早熟禾为MR，高羊茅为MS，牛鞭草为R，剪股颖、狗牙根、多花黑麦草和鸭茅为HS。 相似文献
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为了保护狗舌草资源和实现人工栽培,采用植物组织培养技术,以嫩茎为外植体,进行了愈伤组织诱导与分化、不定芽生根、试管苗继代增殖的培养和试管苗移栽与定植的研究,建立了狗舌草的嫩茎无性系。结果表明：MS＋KT 0.3 mg/L＋2,4-D 1.5 mg/L＋NAA 1.0 mg/L是狗舌草嫩茎愈伤组织诱导培养和继代增殖培养的理想培养基;1/2MS＋IAA 0.2 mg/L＋GA 31.0mg/L是狗舌草颗粒愈伤组织分化培养的理想培养基;把不定芽的下部切口在浓度为1.0 mg/L的NAA溶液中处理2 min后,接种到1/3MS＋IAA 0.2 mg/L～0.4 mg/L的培养基上是狗舌草不定芽生根培养的理想方法;1/3MS＋IAA 0.2 mg/L～0.4 mg/L＋NAA 0.1 mg/L＋GA3 0.4mg/L是狗舌草生根继代增殖培养的理想培养基。试管苗易移栽成活,定植的试管苗保持了野生狗舌草的所有植物学性状。 相似文献
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几种禾草对新月弯孢菌的抗病能力 总被引：1，自引：0，他引：1
以新月弯孢[Curvularia lunata(W akker)Boed ijn]为病原,接种亚热带地区9种常见禾草,观测它们的生长情况、潜育期、发病部位、病斑大小、病斑密度等指标,综合评价供试草的抗感性。结果表明,弯孢叶斑病菌可以侵染供试草种,使禾草生长速度降低、新生叶片形成慢、叶片短而窄、节间缩短,但供试禾草的潜育期(4~10 d,变异系数29.54%)和症状的明显程度不同,潜育期由短到长依次为:剪股颖、狗牙根、高羊茅、牛鞭草、鸭茅、多花黑麦草、多年生黑麦草、一年生早熟禾、假俭草;症状明显程度依次为:剪股颖、狗牙根、多花黑麦草、鸭茅、高羊茅、牛鞭草、一年生早熟禾、多年生黑麦草、假俭草。供试禾草的抗感性:假俭草为高抗(R),多年生黑麦草、一年生早熟禾为中抗(MR);高羊茅为中感(MS);牛鞭草为感病(S);剪股颖、狗牙根、多花黑麦草和鸭茅为高感(HS)。 相似文献
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狗舌草生物碱成分分析 总被引：8，自引：0，他引：8
为了更好地评价狗舌草的药理活性和毒性,利用溶剂萃取法从狗舌草中得到总生物碱,通过色谱和波谱技术对总生物碱进行了分离和鉴定。从TLC及GC-MS判断,狗舌草中至少含有4个双稠吡咯啶生物碱(PAs),GC-MS确定化合物 ～ 分别为千里光宁、千里光非灵和全缘千里光碱。经柱层析和制备性TLC得化合物 ,MS,IR及13CNMR鉴定其为当归酰天芥菜定;化合物 水解产物之一经制备性TLC分离得化合物 ,MS鉴定为天芥菜定。这些化合物的存在说明狗舌草可能具有抗肿瘤活性,并提示其对肝脏的毒性不可忽视。 相似文献
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Conti B Sanchez-Alavez M Winsky-Sommerer R Morale MC Lucero J Brownell S Fabre V Huitron-Resendiz S Henriksen S Zorrilla EP de Lecea L Bartfai T 《Science (New York, N.Y.)》2006,314(5800):825-828
Reduction of core body temperature has been proposed to contribute to the increased life span and the antiaging effects conferred by calorie restriction (CR). Validation of this hypothesis has been difficult in homeotherms, primarily due to a lack of experimental models. We report that transgenic mice engineered to overexpress the uncoupling protein 2 in hypocretin neurons (Hcrt-UCP2) have elevated hypothalamic temperature. The effects of local temperature elevation on the central thermostat resulted in a 0.3 degrees to 0.5 degrees C reduction of the core body temperature. Fed ad libitum, Hcrt-UCP2 transgenic mice had the same caloric intake as their wild-type littermates but had increased energy efficiency and a greater median life span (12% increase in males; 20% increase in females). Thus, modest, sustained reduction of core body temperature prolonged life span independent of altered diet or CR. 相似文献
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[目的]建立栝楼的RAPD-PCR体系并对该体系进行优化。[方法]以栝楼叶片为材料,采用CTAB法提取栝楼叶片基因组DNA,利用正交设计对RAPD-PCR体系进行优化。[结果]各因素水平变化对PCR反应影响大小依次为：Mg2＋、TaqDNA聚合酶、引物和dNTPs。通过试验分析,优化的栝楼RAPD反应体系为：在25μl反应体系中,含10×buffer2.5μl,Mg2＋2.0mmol/L,Taq酶1U,引物0.8μmol/L,dNTPs0.1mmol/L。反应程序为94℃预变性2mim;94℃变性30s,37℃退火温度40s,72℃延伸1.5mim,36次循环;72℃延伸10mim,4℃保存。[结论]该研究建立的栝楼RAPD反应体系稳定可靠,为栝楼性别鉴定、遗传多样性分析、亲缘关系分析等方面的研究提供了有效的方法。 相似文献
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Rapamycin-induced insulin resistance is mediated by mTORC2 loss and uncoupled from longevity 总被引：2，自引：0，他引：2
Lamming DW Ye L Katajisto P Goncalves MD Saitoh M Stevens DM Davis JG Salmon AB Richardson A Ahima RS Guertin DA Sabatini DM Baur JA 《Science (New York, N.Y.)》2012,335(6076):1638-1643
Rapamycin, an inhibitor of mechanistic target of rapamycin complex 1 (mTORC1), extends the life spans of yeast, flies, and mice. Calorie restriction, which increases life span and insulin sensitivity, is proposed to function by inhibition of mTORC1, yet paradoxically, chronic administration of rapamycin substantially impairs glucose tolerance and insulin action. We demonstrate that rapamycin disrupted a second mTOR complex, mTORC2, in vivo and that mTORC2 was required for the insulin-mediated suppression of hepatic gluconeogenesis. Further, decreased mTORC1 signaling was sufficient to extend life span independently from changes in glucose homeostasis, as female mice heterozygous for both mTOR and mLST8 exhibited decreased mTORC1 activity and extended life span but had normal glucose tolerance and insulin sensitivity. Thus, mTORC2 disruption is an important mediator of the effects of rapamycin in vivo. 相似文献
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[目的]为防治栝楼根结线虫提供方法支持。[方法]分别以98%棉隆微粒剂3、5%威百亩水剂、根肥得、甲壳胺0、.5%阿维菌素乳油为供试药剂,在肥城市王瓜店镇进行了防治栝楼根结线虫试验。[结果]用98%棉隆微粒剂203、04、0 g/m2处理后,栝楼病株率分别下降27.34%4、8.00%、53.34%,其防治效果分别达57.38%7、2.73%7、4.24%。用35%威百亩水剂69、1、2 g/m2处理后,栝楼病株率分别下降25.33%4、0.00%3、7.00%,其防治效果分别达50.50%6、7.33%、68.89%。甲壳胺和根肥得的使用剂量不变的条件下,加入4.56、.07、.5 g/m2阿维菌素,栝楼病株率分别下降28.00%4、1.34%、44.00%,其防治效果分别达53.91%、69.53%、71.09%。[结论]使用98%棉隆微粒剂和35%威百亩水剂防治栝楼根结线虫的适宜剂量分别高于30、9 g/m2。在甲壳胺和根肥得的辅助下,使用0.5%阿维菌素乳油防治栝楼根结线虫的适宜剂量在6.0 g/m2以上。 相似文献
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Calorie restriction promotes mitochondrial biogenesis by inducing the expression of eNOS 总被引：2，自引：0，他引：2
Nisoli E Tonello C Cardile A Cozzi V Bracale R Tedesco L Falcone S Valerio A Cantoni O Clementi E Moncada S Carruba MO 《Science (New York, N.Y.)》2005,310(5746):314-317
Calorie restriction extends life span in organisms ranging from yeast to mammals. Here, we report that calorie restriction for either 3 or 12 months induced endothelial nitric oxide synthase (eNOS) expression and 3',5'-cyclic guanosine monophosphate formation in various tissues of male mice. This was accompanied by mitochondrial biogenesis, with increased oxygen consumption and adenosine triphosphate production, and an enhanced expression of sirtuin 1. These effects were strongly attenuated in eNOS null-mutant mice. Thus, nitric oxide plays a fundamental role in the processes induced by calorie restriction and may be involved in the extension of life span in mammals. 相似文献
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Reduced insulin-like signaling extends the life span of Caenorhabditis elegans and Drosophila. Here, we show that, in mice, less insulin receptor substrate-2 (Irs2) signaling throughout the body or just in the brain extended life span up to 18%. At 22 months of age, brain-specific Irs2 knockout mice were overweight, hyperinsulinemic, and glucose intolerant; however, compared with control mice, they were more active and displayed greater glucose oxidation, and during meals they displayed stable superoxide dismutase-2 concentrations in the hypothalamus. Thus, less Irs2 signaling in aging brains can promote healthy metabolism, attenuate meal-induced oxidative stress, and extend the life span of overweight and insulin-resistant mice. 相似文献
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In 12 inbred strains of mice there was no general correlation between litter size and parental life span within strains, although a significant between-strain correlation of +0.69 was found between mean life span of the dams and the mean size of their litters. When data for AKR/J mice, which characteristically die early from leukemia, were excluded, the correlation was increased to +0.90 for the remaining 11 strains. These findings indicate that the correlation is of genetic origin-that is, that genes affecting a dam's life span also affect the size of her litters. 相似文献
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