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1.
Leaf area, IBA concentration and age of stockplants were all found to be important factors for successful rooting for vegetative
propagation using single-node cuttings of the two main African mahogany species: Khaya anthotheca and K. ivorensis. Cuttings with leaf area of 30–50 cm2 had the best rooting percentage and cuttings with about 30 cm2 had the most number of roots per cutting in K. anthotheca. Khaya ivorensis, cuttings with 10–30 cm2 leaf area had the highest rooting percentage. Cuttings collected from 1-year-old stockplants recorded the highest rooting
percentage and largest number of roots per cutting. Cuttings from 3-year-old stockplant of the same seeds sources had the
lowest rooting suggesting aging negatively impacted rooting ability. The effect of auxin concentration, on rootability was
examined with cuttings of K. anthotheca. An IBA concentration of 0.8% was the best exogenous auxin concentration for percentage rooting, number of roots per cutting
and the length of the longest root per cutting. 相似文献
2.
Jonicélia C. Araújo Vieira de Souza Adrián G. Bender Juan C. Tivano Deborah G. Barroso Luis A. Mroginski Abelardo C. Vegetti Peter Felker 《New Forests》2014,45(5):745-752
Mesquite (Prosopis alba Grisebach), an important species in arid and semiarid regions, is currently commercially propagated by seeds and so there is great interest in developing techniques for species propagation, domestication and improvement. Cloning through mini-cuttings is a new and promising technique for the production of clonal seedlings of forest species. The main objective of this work was to evaluate vegetative propagation using the mini-cutting technique and indolebutyric acid (IBA) at different concentrations (0, 3,000, 4,500, 6,000 and 7,500 mg L?1) on rooting of P. alba clones. Rooting was achieved in 98–100 % of the mini-cuttings at all concentrations tested. Increasing IBA concentration resulted in an increase in the number of leaves and leaflets, as well as in fresh matter weight and number and length of roots of clone seedlings, until an optimum point (between 3480 and 4800 mg L?1) was reached. Plants were also vegetatively propagated in the field via conventional clonal garden cuttings under the same rooting conditions to compare the efficiency of the two propagation techniques. Mini-cuttings showed higher rooting and survival percentages than cuttings. A correlation analysis conducted between characteristics of stock plants (height, diameter, number of shoots) and shoots (height, diameter) showed a positive correlation between rooting and height of shoots with total root length, leaflet number, and height of clonal seedlings. We propose the mini-cutting technique as a tool for P. alba cloning. 相似文献
3.
Rooting of baldcypress cuttings 总被引：6，自引：0，他引：6
A test of the rooting performance and growth of cuttings from 1-year-old and 25-to 50-year old baldcypress (Taxodium distichum L.) branch tips is reported. Rooting of cuttings from one-year-old trees averaged 75% (0.1% IBA treatment) and 88% (control), cuttings from 25 to 50-year-old trees averaged 12% in both the IBA treatment and the control treatment. Indole-3-butyric acid at a 0.1% concentration did not enhance rooting, but resulted in a significant increase in shoot dry weight on cuttings from one-year-old trees. The results of this experiment suggest that cuttings from mature baldcypres trees have the potential to root, thereby, additional rooting techniques such as girdling and stump sprouts is worthy of pursuing. 相似文献
4.
Applied auxin, node position, leaf area and cutting length were examined to investigate the requirements for rooting stem cuttings of Khaya ivorensis. All these variables were shown to be important factors affecting rooting, confirming the hypothesis that successful rooting can be achieved if these primary variables are optimised.The best concentration of the auxin IBA was found to be 200 g per cutting, which hastened rooting, increased the percentage of cuttings rooted and increased the number of roots per cutting. One clone (8013) was unresponsive to auxins in terms of the percentage of cuttings rooted, but was the most responsive in terms of the numbers of roots per cutting. A greater percentage of cuttings from basal nodes were rooted than from apical nodes. Cuttings cut squarely at the base produced a radially-arranged root system, whereas an oblique cut resulted in a one-sided root system.Trimming the leaf area of cuttings to 10 cm2 gave greater rooting percentages than trimming to 100 cm2. In general, long cuttings (39 mm) rooted better than short cuttings (19 mm), however, there was an interaction between leaf area and cutting length, in which cuttings with short stems and large leaves had the lowest rooting percentage. 相似文献
5.
Catherine Ky-Dembele Jules Bayala Antoine Kalinganire Fatoumata Tata Traoré Bréhima Koné Alain Olivier 《Southern Forests》2016,78(3):193-199
Pterocarpus santalinoides is a multipurpose tree species, native to Africa and South America. However, excessive pruning for fodder is jeopardising its seed production leading to poor regeneration in the Sahel region. Efficient vegetative propagation techniques may overcome this concern. To address this issue, four experiments were conducted to determine the effects of substrate (Experiment 1), cutting type (Experiments 2 and 3), genotype (Experiment 4) and auxins (Experiments 1, 2, and 4) on rooting ability in a non-mist propagating unit in Mali. Among the four tested substrates, no significant differences were found in rooting parameters, including the percentage of rooted cuttings, the number of roots and secondary roots per rooted cutting, and the length of the longest root per rooted cutting. Semi-hardwood cuttings rooted significantly better than softwood cuttings with a higher percentage of rooted cuttings (31.7?±?6.5% vs. 9.2?±?2.7%), greater number of roots (8.2?±?4.8 vs 1.5?±?0.7), greater number of secondary roots (16.7?±?4.2 vs 4.6?±?1.8) and longer root length (17.8?±?12.0?cm vs 2.3?±?1.0?cm) per rooted cutting. In addition, one-node cuttings cut below the second and third nodes, respectively, had the highest rooting success (88% and 90%, respectively) followed by cuttings cut below the fourth node (68%) and the first node (62%). Significant differences in the percentage of rooted cuttings were found among 12 randomly selected genotypes with rooting varying from 2% to 75%.The results also showed that auxin application, especially IBA, had a significant effect on rooting success, the number of roots and secondary roots per rooted cutting. Single-node cuttings, and cuttings of 10?cm length with multiple softwood and semi-hardwood nodes, can be used with or without auxin treatment for successful clonal propagation of P. santalinoides. The results of the present study indicated that P. santalinoides is amenable to clonal propagation using low-cost propagators and sand-based rooting media. 相似文献
6.
A study to determine the best vegetative propagation protocol was carried out for Dalbergia melanoxylon. Natural regeneration of D. melanoxylon is limited due to poor seed viability and germination. The effects of age of donor plant, indole-3-butyric acid (IBA) treatment
and cutting position on rooting of stem cuttings were investigated. The effect of age of donor plant was significant (P < 0.001) only for percent rooting, position effect was significant for all studied parameters, and IBA treatment at 300 ppm
was insignificant only for percent callusing. Interactive effects of cutting position × age of donor and cutting position × IBA
treatment were significant for percent rooting and root number per cuttings while interactive effect of age × IBA was significant
only for percent rooting. Observed difference in rooting performance could be due to uneven distribution of root promoting
and inhibiting substances with respect to age of donor plant and IBA which intensified root formation. Cuttings from juvenile
donor plant performed better in all rooting parameters than mature donor plant. The highest percentages in rooting were (71.11%)
and (24.42%) for cuttings from juvenile and mature donor plants, respectively. IBA treated cuttings produced higher percentage
rooting, number of roots and root length than untreated cuttings which revealed a strong IBA influence on rooting ability
of stem cuttings in D. melanoxylon. It was concluded that stem cuttings should be taken from juvenile donor plants and basal cutting position should be used
to produce high quality planting stock material for the afforestation and conservation programme of D. melanoxylon. 相似文献
7.
The main objective of our clonal propagation program is the production of high quality timber and faster tree growth. Teak
(Tectona grandis Linn. f.) is an important plantation species in the tropics but the demand of teak timber is increasing globally. Teak planting
stock is still produced from seeds of unselected elite genotypes which may lead to poor stand establishment. The number of
seeds per fruit and their germination ability are also variable and limited by several factors. Therefore an alternative method
i.e., clonal propagation is required for mass multiplication of elite trees. It is an established fact that the clonal propagation
or adventitious root formation in cuttings is markedly affected by several external and internal factors. Considering these,
an experiment was conducted on leafy soft wood shoot cuttings of 3-year-old hedged teak stock plants grown in a hedge garden
to study the effects of branch position and auxin treatment on adventitious root formation. A factorial completely randomized
design was used for experimentation. Coppice shoots were collected from basal, middle and upper positions of the hedge garden;
and mono-nodal leafy cuttings were prepared. These cuttings were treated with different concentrations of IBA and NAA; and
were cultured in a mist chamber for rooting where the relative humidity was maintained at 85 ± 2% with maximum and minimum
day-night temperature at 32 ± 1°C and 26 ± 1°C respectively. After 45 days the cuttings were removed from the rooting medium
and observations were made. It was observed that the rooting parameters viz., percent rooting and percent sprouting, mean
number of leaves, mean number of shoots and length, and mean number of roots and length per cutting were significantly influenced
by the branch position and auxin treatment. Treatment with 4000 ppm IBA increased percent rooting and percent sprouting whereas
NAA suppressed it. Treatment with 4000 ppm IBA also increased the mean number of leaves and shoots, and the length, and the
mean number of roots per cutting. The highest mean number of roots and length were observed in cuttings taken from the branch
emerging at the upper position followed by branches of middle and lower position. Cuttings of branches at a middle position
showed the highest values of percent rooting, percent sprouting, mean number of leaves and shoots and mean shoot length per
cutting. Furthermore, the interactive effect of branch position and auxin treatment on rooting and sprouting response was
also found to be significant. Overall, the findings of this investigation suggest that middle branch position in hedge plants
and 4000 ppm IBA can be made to induce maximum rooting and to produce high quality planting stock material for clonal forestry
program of teak. 相似文献
8.
This study investigates the optimal conditions for the vegetative propagation of the multipurpose leguminous shrub Colutea istria from leafy stem cuttings. The conditions examined included 1) the origin of the cutting (apical, medial or basal), 2) indole-3-butyric
acid (IBA) treatment (0, 50, 200 or 400 mg/l), 3) the relative humidity of the propagation environment (high or low), 4) wounding
base of cutting, and 5) the season in which the cutting was taken. The rooting ability of the cuttings was significantly influenced
by all these factors. Winter cuttings obtained from the middle and base of the branch showed a greater rooting capacity than
those harvested from the apical area, while autumn cuttings harvested from the middle and apical areas of the branch showed
a greater rooting capacity than those harvested from the base. Winter cuttings showed the best rooting capacity than autumn
cuttings. IBA treatment (200 mg/l), high humidity (98-100%), and wounding increased the rooting capacity of the cuttings. The sprout
of new leaves was higher in basal cuttings, while IBA and wounding also significantly enhanced the root number of rooted cuttings.
A method for vegetatively propagating C. istria plants is given. 相似文献
9.
Liliana Muñoz-Gutiérrez J. Jesús Vargas-Hernández Javier López-Upton Marcos Soto-Hernández 《New Forests》2009,38(2):187-196
Taxus globosa (Mexican yew) is a conifer endemic to México and Central America. It produces a substance known as taxol, which is useful
in treatment of ovarian cancer. Because seed production for this dioecious tree is limited, and seed germination is extremely
difficult, the use of cuttings could facilitate propagation of this species. With the intention of massively propagating individuals
selected for taxol content, two trials were established in which the effect of substrate temperature (average temperatures
18 and 23°C), age (i.e., young vs. old shoots) and management of cuttings, as well as clone variation in rooting capacity,
were evaluated. Low temperature favored rooting (53 vs. 34% on average for the two trials); younger shoots rooted three times
(61 vs. 23%) more than mature ones, while basal wounding did not affect rooting capacity. A wide variation was found in rooting
capacity of clones (8–76%), which could be associated with genetic or physiological differences among donor trees. 相似文献
10.
11.
Vegetative propagation of Prunus africana: effects of rooting medium, auxin concentrations and leaf area 总被引：2，自引：0，他引：2
Z. Tchoundjeu M. L. Avana R. R. B. Leakey A. J. Simons E. Assah B. Duguma J. M. Bell 《Agroforestry Systems》2002,54(3):183-192
The region of West and Central Africa is endowed with high-value fruit trees and medicinal plants, which are currently traded
locally as well as on regional and international markets. Unfortunately, they are all exploited from the wild and there has
been little or no focussed effort to domesticate and cultivate them. Prunus africana is one of these important medicinal plant under domestication. A series of nursery experiments were conducted to assess the
effects of rooting medium (sawdust, sand and a 50:50 mixture of sand and sawdust), auxin concentration (0, 50, 100, 150, and
200 μg IBA), and leaf area (0, 5, 10, 20, and 25 cm2) on rooting success of juvenile cuttings of P. africana. The percentage of cuttings rooted was significantly greater (P < 0.05) in sawdust (80%), than in sand alone (72%) or in mixture with sawdust (71%). Leaf area also significantly affected
the percentage of rooting. Leafless cuttings did not root and were all dead by week 6, but in leafy cuttings rooting ability
increased proportionally with leaf area up to 20 cm2 (79%). Larger leaf cuttings (25 cm2) rooted at the same level as those of 20 cm2. The cuttings with the largest leaves also had the greatest mean number of roots per cutting (14 roots cutting−1), while those with the smallest (5 cm2) leaf area produced the fewest roots (5 roots cutting−1). The application of auxin (IBA) promoted rooting (P < 0.05) up to an optimum application of 100–200 μg IBA per cutting, but 300 μg was supraoptimal. It can be concluded that
P. africana is amenable to vegetative propagation.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
12.
The cutting propagation technique ofPicea koraiensis was carried under automatic spraying of total sunlight by adopting the same year epicormic branches from different trees
with different age treated by different hormones in different concentration and time and the orthogonal and randomized block
was designed. The results were as follows: (l)The rooting rate of the cutting reached at least 87 percent, when the cuttings
were from the seed tree with the age below 25 years old and the cuts of the cuttings base were in vertical angle and on the
fine sand medium. (2) The sequence of effective factors affecting the rooting rate were the style of cut, the age of seed
tree and the type of medium. (3) There are significant differences of rooting rate among different clones. 相似文献
13.
R. L. Jinks 《New Forests》1995,10(2):183-195
The effect of propagation environment on the rooting of field collected leafy cuttings of ash (Fraxinus excelsior, sycamore (Acer pseudoplatanus) and sweet chestnut (Castanea sativa) was investigated in two experiments. In the first experiment, rooting of all three species in mist enclosed inside a polythene tent was greater than 50%, while rooting of ash cuttings was reduced from 63% in enclosed mist to only 16% in open mist. Rooting in open mist, enclosed mist, and contact polythene was studied in a second experiment. Sycamore cuttings rooted equally well in the two mist systems with an average rooting of 78%. In contrast cuttings of both ash and sweet chestnut rooted most under the enclosed mist system, at 64 and 46% respectively. Rooting of ash cuttings was again depressed in the open mist system where less than 30% of cuttings rooted. This reduction was related to an increase in the percentage of cuttings which remained alive without rooting, while showing proliferation of callus at the cutting base. The percentage of sweet chestnut cuttings which callused without rooting was also much greater in the open mist system than in enclosed mist or contact polythene. 相似文献
14.
A rapid vegetative propagation method is described for Olea europaea L. subsp. cuspidata [Wall. ex DC.) Ciffieri (syn. Olea
africana Mill.)]. Leafy branch cuttings were harvested from 6 to 7-month-old stecklings (= plants derived from rooted cuttings). Cuttings 2–3 mm in basal diameter were trimmed to 70–100 mm in length, with leaf areas reduced to 15–33 cm2. Indolebutyric acid (IBA) was applied at 0, 10, 20, 25, 30, 35 and 40 µg/cutting to each of the 70–80 replicate cuttings randomly allocated to each of the 7 treatments. Callusing and rooting occurred 3–5 and 5–7 weeks after treatment, respectively. The rooting success of cuttings treated with 20 or 40 µg IBA/cutting was 75 and 90%, respectively. Differences in rooting success, speed of rooting and root numbers between the control and the IBA treatments were significant (p < 0.01). Compared to seedlings of similar shoot height, rooted cuttings grew significantly (p < 0.01) faster and produced more biomass. It is concluded from this study that the rooting of leafy branch cuttings derived from young stockplants of wild olive is rapid, and that vegetative propagation is an effective means of regenerating this valuable, yet threatened, tree species. 相似文献
15.
Celastrus paniculatus Willd. (Celastraceae) is an important medicinal tree valued for its seeds. Indiscriminate collection of this plant from the
habitat zones has posed a serious threat to its existence in the wild, results in globally a threatened plant species. Ex
situ conservation is a prioritized agenda of research for redlisted plant species. Semi-hardwood stem cuttings collected from
10 to 15 year old trees were treated with different concentrations (0, 0.5, 1.0, 2.0, 3.0, 4.0 and 5.0 g l−1) of growth hormones; indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) and 1-naphthalene acetic acid (NAA) for positive
effect on root induction. After 90 days of treatment, results were analyzed for percentage of rooting, root number and average
root length per rooted cuttings. The highest rooting (57%) response with highest number of roots (~77.2) was obtained in indole-3-acetic
acid at 3.0 g l−1. Overall, high significant (P ≤ 0.05) rooting response was noticed in IAA treatment comparative to hormones IBA and NAA. Rooted cuttings exhibited 100%
survival in the experimental field. This study reveals the propagation of semi-hardwood cuttings derived from partially matured
branches is possible and it provides a basis for conservation of this threatened plant species by reducing pressure on its
natural populations. 相似文献
16.
Propagation of subtropical eucalypts is often limited by low production of rooted cuttings in winter. This study tested whether changing the temperature of Corymbia citriodora and Eucalyptus dunnii stock plants from 28/23°C (day/night) to 18/13°C, 23/18°C or 33/28°C affected the production of cuttings by stock plants, the concentrations of Ca and other nutrients in cuttings, and the subsequent percentages of cuttings that formed roots. Optimal temperatures for shoot production were 33/28°C and 28/23°C, with lower temperatures reducing the number of harvested cuttings. Stock plant temperature regulated production of rooted cuttings, firstly by controlling shoot production and, secondly, by affecting the ensuing rooting percentage. Shoot production was the primary factor regulating rooted cutting production by C. citriodora, but both shoot production and root production were key determinants of rooted cutting production in E. dunnii. Effects of lower stock plant temperatures on rooting were not the result of reduced Ca concentration, but consistent relationships were found between adventitious root formation and B concentration. Average rooting percentages were low (1–15% for C. citriodora and 2–22% for E. dunnii) but rooted cutting production per stock plant (e.g. 25 for C. citriodora and 52 for E. dunnii over 14 weeks at 33/28°C) was sufficient to establish clonal field tests for plantation forestry. 相似文献
17.
通过双因素试验设计对4种不同生根促进剂种类（H）、6种不同生根促进剂浓度（S）对厚皮香容器扦插生根率的不同影响力进行了研究,同时还研究了5种不同扦插基质（G）及3种不同母株部位剪切的穗条（X）对厚皮香容器扦插生根率的影响差异。结果表明：不同的生根促进剂种类和不同生根促进剂浓度对厚皮香扦插生根率的促进作用是显著的,生根促进剂种类对扦插生根率的影响力大于生根促进剂浓度,以采用质量分数为750×10-6（S5）的IBA（H3）处理,厚皮香插穗生根率高,达85.4%;基质及母株采穗部位对厚皮香扦插生根率的影响显著,母株采穗位对厚皮香扦插生根率的影响力大于基质,以母株下部（X3）剪切的插穗扦插到各50%的红壤和珍珠岩（G2）基质时,生根效果最好,平均生根率达87.9%。 相似文献
18.
比较不同基质、不同生根促进剂及其质量体积浓度、不同季节、不同修剪方式、不同插穗类
型对钟花樱（Cerasus campanulata）扦插生根的影响,结果表明：扦插基质类型、生根激素种类及其质量
体积浓度、扦插季节、插穗留叶面积以及插穗类型均对钟花樱扦插成活率和生根率产生显著或极显著影
响,扦插基质采用黄心土最有利于生根;生根激素种类及其各浓度处理效果均显著高于清水对照,其中
采用IBA 以质量体积浓度2 500 mg · L-1 速蘸10 s 处理效果最佳;于春季扦插最好;插穗留1 片或半片叶
片成活率及生根率远高于不留叶插穗;采用嫩枝扦插和带踵扦插优于硬枝扦插。因此钟花樱扦插宜在春
季,采用修剪成1 片或半片叶片带踵或嫩枝的插穗,经质量体积浓度2 500 mg · L-1 的 IBA 药液蘸10 s 后
扦插在黄心土上,生根效果最好。 相似文献
19.
20.
Fett-Neto AG Fett JP Veira Goulart LW Pasquali G Termignoni RR Ferreira AG 《Tree physiology》2001,21(7):457-464
Adventitious rooting is essential for vegetative propagation of woody species. We studied the effects of auxin and light on the development of adventitious roots in cuttings obtained from seedlings of Eucalyptus saligna Smith and E. globulus Labill in an attempt to characterize the adventitious rooting process and identify factors controlling rhizogenesis. Root development was scored as rooting percentage, root density (roots per rooted cutting), mean rooting time and root length. In both species, rooting time was reduced in the presence of auxin. Cuttings from 2-month-old E. saligna seedlings were responsive to lower auxin concentrations than comparable cuttings from E. globulus seedlings. Cuttings from 3-month-old E. saligna seedlings rooted promptly and rooting was not significantly affected by light conditions. In contrast, rooting of cuttings from 3-month-old E. globulus seedlings exhibited recalcitrant behavior and no roots were formed if illuminated during the root formation phase. Effective root regeneration of E. globulus cuttings was obtained by a 4-day exposure to 10 mg l(-1) IBA and culture in darkness during the root formation step. Loss of rooting capacity with seedling age was more pronounced in E. globulus than in E. saligna. The possibility of switching adventitious rooting off and on by manipulating light regime and exogenous auxin supply in E. globulus, and the constitutive nature of rooting in E. saligna may provide useful models for examining the rooting process at the biochemical and molecular levels in Eucalyptus. 相似文献