This study aims to analyse the influence of adding natural zeolites (clinoptilolite) to the diet or litter of broilers and their effects on growth performance, carcass yield and litter quality.
Three consecutive flocks of broilers were raised on the same sawdust litter, from d 1 to d 42 of age, and distributed in three treatments (control with no added zeolites, addition of 5 g/kg zeolite to diet and addition of 100 g/kg zeolites to litter).
The addition of zeolites to the diet or litter did not affect growth performance or carcass yield.
The addition of zeolites to the diet did not influence moisture content of the litter, ammonia volatilisation was reduced only in the first flock and pH of litter was reduced in the second and third flock.
However, the addition of zeolites to the litter reduced moisture content, litter pH and ammonia volatilisation in all flocks analysed.
The addition of 5 g/kg zeolite to the diet in three consecutive flocks was not effective in maintaining litter quality, whereas the addition of 100 g/kg natural zeolites to sawdust litter reduced litter moisture and ammonia volatilisation in three consecutive flocks raised on the same litter.
The objective was to investigate inbreeding depression for some economic traits of Mazandaran native fowls using data collected from 1992 to 2012 (21 generations) using a REML animal model of significant fixed and random effects with inbreeding of birds and dams as covariates.
The mean inbreeding coefficient (F) for the whole population and dams was 4.67% and 4.12%, respectively, and most of the inbred birds (75.79%) and inbred dams (72.58%) had F < 12.5%.
Individual and dam inbreeding trends were 0.55% and 0.53% per year.
Inbreeding depression for body weight at hatch, at 8 weeks and 12 weeks of age, age at sexual maturity, weight at sexual maturity, egg weight at 1st d of laying and average egg weight at 28, 30 and 32 weeks of laying due to a 1% increase in individual inbreeding were ?0.11 g, ?3.1 g, ?1.3 g, 0.15 d, 0.59 g, ?0.05 g and ?0.03 g, respectively.
A 1% increase in maternal inbreeding resulted in a reduction of 0.06, 0.6 and 3.6 g in body weight at hatch, 8 weeks and 12 weeks of age.
In this study, the effect of chlorogenic acid extract from Lonicera japonica Thunb. on Mycoplasma gallisepticum infections and the performance of broiler flocks was investigated.
A total of 360 Ross-308 broiler chicks taken from M. gallisepticum seropositive flocks were divided equally into three groups designated as control (nothing administered), antibiotic (Tylosin tartrate given for the first 3 d and d 20–22) and test group (chlorogenic acid extract given twice a day on d 16 and 22).
Broiler performance analysis, serological tests (slide agglutination), molecular identification (polymerase chain reaction) and histopathological examination were performed to detect M. gallisepticum.
The results show that chlorogenic acid not only increases live body weight but is also an alternative treatment option in M. gallisepticum–infected broiler flocks.
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Regardless of breed, organ weight does not change at the same rate as body weight during the first 8 weeks of life.
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The ratio of liver or heart weight to body weight reaches a peak at about one to two weeks of age and then decreases gradually with age until 8 weeks. Spleen‐body weight ratio, however, increases to about the 3rd week of age after which it remains relatively constant.
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Significant negative correlations were obtained between body weight and both liver‐ and heart‐body weight ratios; while significant positive correlations were obtained between body weight and spleen‐body weight ratios. The correlation between liver weight and heart weight was found to be higher than that between liver weight and spleen weight or between heart weight and spleen weight.
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The ratio of organ weight to body weight differed with different breeds. The faster growing breeds had lower ratios of organ weight to body weight and higher correlation coefficients between body weight and organ weight ratios.
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Regression equations are presented that enable the estimation of organ weights from body weights or from other organ weights in the three different breeds and two breed crosses studied.
This study aimed to evaluate the changes in physicochemical, textural and sensory characteristics of broiler (BR) and Taiwan native chicken (TNC) pectoralis muscle heated at temperatures of 50–95°C.
With increasing temperature, cooking loss, collagen solubility, shear force value and hardness, of samples increased in both chicken breeds. Rapid decreases in protein solubility were observed when the meat was heated to 50°C and gradually decreased thereafter.
Meat from BRs and native chickens performed differently upon heating in certain characteristics. TNC meat had longer cooking time and lower myofibrillar fragmentation index than BR meat did. TNC meat had higher collagen content, shear force values and springiness but lower collagen solubility than BR meat did.
BR meat had significantly higher onset and end transition temperatures than TNC meat did. In BR meat, a sensory analysis revealed that moisture release decreased and chicken flavour increased with increasing temperature.
Protein solubility, cooking loss and the texture of heated meat were highly correlated.
This study scientifically assessed the performances of the two breeds of chickens with different thermal treatments; producers could utilise the information to produce poultry products with more desirable qualities.
This study examined the changes in eggshell strength and the genetic parameters related to this trait throughout a hen’s laying life using random regression.
The data were collected from a crossbred population between 2011 and 2014, where the eggshell strength was determined repeatedly for 2260 hens.
Using random regression models (RRMs), several Legendre polynomials were employed to estimate the fixed, direct genetic and permanent environment effects. The residual effects were treated as independently distributed with heterogeneous variance for each test week.
The direct genetic variance was included with second-order Legendre polynomials and the permanent environment with third-order Legendre polynomials.
The heritability of eggshell strength ranged from 0.26 to 0.43, the repeatability ranged between 0.47 and 0.69, and the estimated genetic correlations between test weeks was high at > 0.67.
The first eigenvalue of the genetic covariance matrix accounted for about 97% of the sum of all the eigenvalues.
The flexibility and statistical power of RRM suggest that this model could be an effective method to improve eggshell quality and to reduce losses due to cracked eggs in a breeding plan.
The trial was performed with 80 laying hens belonging to two Tuscan autochthonous breeds: 40 birds of the Mugellese (MU) breed and 40 of the White Leghorn (WL) breed.
The animals were allotted to 4 groups of individually caged 20 hens each: two groups were fed on a commercial diet and worked as the control groups (MUC and WLC); the other two groups received the same diet, integrated with 2 g of chestnut tannin (CT) extract per kg of diet (MUT and WLT).
A sample of 70 eggs were randomly collected and analysed for cholesterol content, fatty acid (FA) profile, weight, thickness of shell and colour of yolk.
Physical parameters, including yolk colour, and indices of egg quality were not affected by the treatments.
The concentration of unsaturated FAs increased, whereas cholesterol was significantly decreased: ?17% in WLT and ?9% in MUT. Dietary supplementation with CT extract resulted in a modification of lipid composition, towards a more healthy quality of eggs.
The onset and progression of Salmonella infections was investigated in commercial turkey flocks from placement at 1 d old until slaughter in “brood and move” systems using a longitudinal observational approach based on faeces and environmental sampling with subsequent culture of Salmonella.
Persistent Salmonella Newport contamination was found within rearing houses and on their external concrete aprons after cleaning and disinfection between crops of heavily shedding young birds.
Salmonella shedding was often detected by 5 d of age and the frequency of positive samples peaked at 14–35 d. Thereafter Salmonella isolations declined, especially in the later (fattening) stages. Samples were still Salmonella-positive at low prevalence in half of the intensively sampled houses at slaughter age.
A number of management interventions to combat Salmonella infection of flocks, including sourcing policy, competitive exclusion cultures and cleaning and disinfection, were inadequate to prevent flock infection, although improved disinfection on one unit was associated with a delay in the onset of flock infection.
The objective of the study was to investigate the distribution of LPIN2 variants and haplotypes among breeds and perform an association analysis of the variants and haplotypes with the broiler traits in chickens.
Six breeds were used to study the variation and distribution of chicken LPIN2, and an F2 resource population was used to measure growth traits, carcass traits, meat quality traits and serum biochemistry parameters.
A c.-599G>A variant was located in the promoter region of LPIN2 and c.444G>A and c.1730A>T (E577D) coding variant mutations were detected. Linkage disequilibrium tests showed that these three variants were under moderate linkage disequilibrium in the 6 breeds and 7 haplotypes were constructed. The distribution of variation/haplotypes presented clear differences among breeds.
Association analysis showed that c.-599G>A was associated with leg muscle weight, jejunum length, ileum length, leg muscle fibre density and leg muscle fibre diameter; c.444G>A was associated with spleen weight, ileum length, body weight at hatch and metatarsus length at 8 weeks; c.1730T>A had significant effects on chicken liver weight, heart weight, body weight at 10 weeks, serum albumin and glucose.
Diplotypes were significantly associated with body weight at hatch, heart weight, pancreas weight, duodenum length, leg muscle fibre density and lactate dehydrogenase.
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? Semen and blood samples were collected from 541 rams in 40 flocks.
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? The type of antigen which has been used for a number of years in New Zealand was unsatisfactory. The CCFT using this antigen had a sensitivity of only 85% in 124 rams which gave positive semen cultures. These rams came from 16 infected flocks.
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? The CCFT using the best of the 3 antigens proved to be a remarkably reliable test. In the same group of infected animals mentioned above, 120 of the 124 infected rams had positive or suspicious CFT's. The sensitivity of the test was therefore 97%. The specificity was > 99% in 144 rams in 16 uninfected flocks. Only 1 suspicious titre was found in the uninfected group.
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? In 4 flocks in which B. ovis was never isolated, 18 of 58 sera gave positive or suspicious reactions. Recognition of this type of 'problem flock' is therefore important. A history and clinical picture which does not agree with serological results should always be thoroughly investigated. Semen should be collected from an appropriate number of cases for bacteriological examination. It is suspected that at least some ‘problem flocks’ may contain unreported vaccinated animals. Further investigation of ‘problem flocks’ will be an aim of the Animal Health Division in the future.
A study of broiler breeder eggs differing in eggshell matrix optical density was conducted to determine the association of eggshell structural quality and the risk of disease in broilers.
A total of 10 000 eggs from a broiler breeder flock were examined according to the pre-established criteria, and allocated to groups classified as having a high or low density shell matrix.
The eggs from respective groups were incubated and hatched in a commercial hatchery. Samples of unhatched eggs from each group were subjected to detailed examination to establish the cause of reproductive failure. First, quality chicks from each group were raised as separate flocks in a commercial broiler barn. Group performance, morbidity, and mortality were monitored throughout the growth period. All birds were processed in a commercial plant, and condemnation data were compiled.
There were significantly more unhatched eggs, and fewer quality chicks in the group classified as having a low density eggshell matrix, in comparison to the high-density group.
Embryo pathology accounted for a large proportion of the overall reproductive failure in both groups, with a large proportion of embryos showing anatomical anomalies. The eggs and embryos from the low-density eggshell matrix group were three times more likely to be infected.
Significantly higher production losses associated with mortality/morbidity, and condemnations of carcasses at processing were observed in broilers from the low-density eggshell group compared with the high-density eggshell group.
It is concluded that common metabolic/physiological changes in breeder hens associated with eggshell pathology may be also risk factors linked with predisposition of broiler chicks to some health problems.
The relationships between litter moisture, footpad dermatitis (FPD) and pain in medium-heavy turkey strains was studied by gait analysis in two medium-heavy with and without analgesia (betamethasone or bupivacaine).
The relationship between FPD and litter moisture was linear above a breakpoint of 49% litter moisture, and there were no differences between the two breeds in susceptibility to FPD.
Gait analysis showed higher impulse, single support time, stride time and stance time in breed A compared to breed B. Significant interactions between breed, litter and analgesic for impulse, single support time and stride time were associated with higher means for breed A given saline injection on wet litter.
Data from betamethasone analgesia in Experiments 1 and 3 were combined for analysis. Peak vertical force was higher in saline- compared to betamethasone-treated birds. Compared to the wet (high FPD) litter treatments, birds on dry (low FPD) litter had greater speed and lower double support time and longer stride length. Turkeys kept on wet litter had a longer stride length compared to dry litter when given saline, whereas in betamethasone-treated birds the means were similar.
There were no differences between birds with or without bupivacaine analgesia. Peak vertical force was higher in breed A than B and in birds with a low FPD compared to a high FPD score.
It was concluded that breeds A and B did not differ in susceptibility to develop FPD when housed on wet litter but may have natural gait differences. Significant changes in gait parameters were associated with wet litter and with analgesic treatments. The results showed that FPD affected the gait of the turkeys and, combined with evidence of behavioural changes when given analgesia, suggest that footpad lesions are painful.
Milanino is a heavy Italian chicken breed included in a conservation project of the University of Milan and is an important genetic resource for alternative production systems. This research was aimed to study the effect of the dietary protein concentration on growth, slaughter performance and meat composition in free-range reared Milanino chickens.
A total of 120 Milanino chickens were fed on different protein concentrations (HP = 20% CP and LP = 16% CP), reared according to a free-range system and slaughtered at 150 and 180 d of age. Growth, slaughter performance and meat (breast and thigh) composition were recorded.
The protein concentration of the diet did not affect the overall Milanino mean body weight recorded in the straight-run group in the whole rearing period. However, the growth rate within sex was significantly different between the dietary treatments: heavier females were found in the HP group from 125 d onwards, while no differences were recorded in male body weights. The protein concentration of the diet did not affect carcass weight data or meat composition.
The present results suggest the use of a low-protein diet for rearing straight-run Milanino chickens for long rearing periods. However, in females, a high-protein diet is recommended from 125 d of age onwards.
Genetic parameters were estimated for 5 economically important egg production traits using records collected over 9 years in chickens reared under tropical conditions in Thailand. The data were from two purebred lines and two hybrid lines of layer parent stocks.
The two purebred lines were Rhode Island Red (RIR) and White Plymouth Rock (WPR) and the hybrid lines were formed by crossing a commercial brown egg laying strain to Rhode Island Red (RC) and White Plymouth Rock (WC), respectively.
Five egg production traits were analysed, including age at first egg (AFE), body weight at first egg (BWT), egg weight at first egg (EWFE), number of eggs from the first 17 weeks of lay (EN) and average egg weight over the 17th week of lay (EW).
Fixed effects of year and hatch within year were significant for all 5 traits and were included in the model.
Maternal genetic and permanent environmental effects of the dam were not significant, except for EN and EW in RIR and BWT and EW in WPR.
Estimated heritability of AFE, BWT, EWFE, EN and EW were 0.45, 0.50, 0.29, 0.19 and 0.43 in RIR; 0.44, 0.38, 0.33, 0.20 and 0.38 in WPR; 0.37, 0.41, 0.38, 0.18 and 0.36 in RC; and 0.46, 0.53, 0.36, 0.38 and 0.45 in WC lines, respectively.
The EN was negatively correlated with other traits, except for BWT in RC and AFE and BWT in WC.
It was concluded that selection for increased EN will reduce other egg production traits in purebred and hybrid chicken and therefore EN needs to be combined with other egg production traits in a multi-trait selection index to improve all traits optimally according to a defined breeding objective.
The objective of this study was to identify any issues arising during the laboratory testing of samples collected under the National Control Programme for Salmonella.
A questionnaire-based survey was conducted among Defra (Department for Environment, Food and Rural Affairs)-approved testing laboratories in order to identify any complicating factors that the laboratories may encounter during the processing of samples.
Samples were reported to arrive in good condition and within the specified time frame after collection. The only concern was that new clients may be unaware of the procedure or correct sampling consumables to use.
There was evidence of variability between laboratories regarding the sample testing procedure used, with deviation from the guidelines in some cases.
This finding suggests that further guidance for laboratories on methodology is likely to be beneficial as this could help improve the detection of low levels of Salmonella.
LPIN1 is a Mg2+-dependent phosphatidic acid phosphatase. Variation in chicken LPIN1 exon 6 and its flanking regions were identified and three indel variants in 6 breeds and their associations with performance traits were studied.
Seven variants were detected from 6 breeds, which contained a synonymous tri-allelic variant (c.924A/T/C) and three indels. The exon 6 variants detected from chicken breeds were conserved among bird species. The indel variation frequency presented clear differences among breeds.
Two coding indels (c.1014-1018del3 and c.1125-1138del12) were multiples of three nucleotides and maintained the open reading frames of LPIN1 proteins. However, they were predicted to result in the clear change of the RNA secondary structure of chicken LPIN1 exon 6 and LPIN1 protein conformation.
The association analysis showed that c.871–15-22del6 variation had a significant effect on body weight at hatch (BW0) and 2 weeks (BW2); c. 1014-1018del3 variation had a significant effect on BW4, BW6, caecum length and gizzard weight (GW) traits; c.1125-1138del12 variation had a significant effect on BW12, shank length at 4 weeks (SL4), carcass weight, lactate dehydrogenase traits (LDH), glucose (GLU) and albumin (ALB) traits. The genotype combination for c.1014-1018del3 and c.1125-1138del12 also presented significant effects on SL4, SL8, GW, leg muscle weight, ALB, GLU and LDH.
The study demonstrated that chicken LPIN1 has an important effect on body, carcass and organ weight, serum LDH, GLU and ALB level.
1. Guinea fowl hens were inseminated weekly once with two doses of spermatozoa (75 million and 100 million) in two different diluents, Beltsville poultry semen extender (BPSE), and Instruments for Veterinary Medicine (IMV), each with and without pre-insemination vaginal douching. Per cent fertility, hatchability, dead germ, dead in shells along with data on sperm egg interaction and vaginal microbial counts were recorded.
2. Artificial insemination had significantly improved the per cent fertility and hatchability compared to natural mating. Dose dependent improvement in fertility was noticed with both diluents.
3. There was a beneficial effect of vaginal douching, which was more pronounced at lower insemination doses.
4. For optimum fertility and hatchability in guinea fowl, insemination of 75 million spermatozoa diluted in BPSE once in 4 d and 100 million spermatozoa diluted in BPSE or IMV once in 5 d coupled with vaginal douching is recommended.
The aim of this study was to analyse the morphological lesion pattern of the heart of broiler chickens (Cobb 500, Hubbard F15 and Ross 308) during fattening with no clinical signs of disease and to determine the most susceptible period for the occurrence of morphological lesions.
The most frequently diagnosed lesions in each genetic line were degeneration of the fibres with vacuolation, congestion of cardiac muscle, oedema and vacuolisation of the Purkinje cells.
The highest numbers of morphological lesions were observed on d 38, 31 and 10 of life. The lesions were most numerous in the septum, followed by the left and right ventricles.
Ischaemic cardiomyocytes were also most numerous on d 38 of life and in the left ventricle.
Overload of cardiac muscle, prolonged hypoxia and increasing body weight on d 38 are the likely reasons for the largest number of lesions and ischaemic fibres, which may lead to heart failure.
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the incidence of free‐range, deep‐litter and battery husbandry in flocks from which they came, as estimated by interpolation from annual census data;
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season, as represented by fundamental and first‐harmonic sine and cosine terms;
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age‐structure of the flocks, as indicated by the incidence of extra‐small eggs 10 months earlier;
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time;
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packing‐station throughput.
It is shown that 93–3 per cent of the variance of downgrading percentage is associated with variation in (i), (ii) and (iii). After these effects had been taken into account there was no significant long‐term time‐trend but there were small effects associated with certain years. There was no significant effect of (v). The regression equation was used to predict the incidence of downgrading in the second week of each month from April 1968 to January 1969; this it did accurately except in May and June 1968, when the downgrading percentage fluctuated to an unprecedented degree following a very cold and a very hot spell of weather.
It is suggested that large producer‐packer groups should systematically collect information on the incidence of downgrading and the percentages of birds of each strain and age‐group, of cages of each type, of nutritional regimes and of egg‐collection methods; and that these data should be analysed to yield (a) forecasts of downgrading incidence and (b) estimates of the effect of each strain, cage‐type, etc. on downgrading incidence, for use when deciding replacement policy. 相似文献
This study had the aim of evaluating the effects of medium-chain fatty acids (MCFA) on performance, carcass characteristics, some blood parameters and antibody titre against sheep red blood cells (SRBC) in quail.
A total of 240 quail chicks were allotted to 4 treatments consisting of respectively 0, 1, 2 and 4 g/kg dietary MCFA.
There were no significant differences in body weight, feed intake and feed conversion ratio among treatments at different stages of the experiment.
MCFAs had no significant effect on breast, thigh, liver, spleen and bursa of Fabricius weight ratios. However, the relative weight of abdominal fat significantly decreased in quail receiving 0.2 and 0.4 MCFA as compared to other treatments.
Concentrations of low-density lipoprotein-cholesterol (LDL), triglycerides and total cholesterol were decreased and high-density lipoprotein (HDL)-cholesterol was increased in quail chicks receiving MCFA compared with control quail chicks. The concentrations of SRBC antibody were not statistically different among treatments.
It is concluded that MCFA significantly decreased LDL, triglycerides, cholesterol and abdominal fat and increased HDL in quail chicks.