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1.
Three experiments were conducted to evaluate effects of supplemental protein vs energy level on dormant forage intake and utilization. In Exp. 1, 16 ruminally cannulated steers were blocked by weight (avg wt = 242 kg) and assigned randomly to a negative control or to one of three isocaloric supplement treatments fed at .4% BW: 1) control, no supplement (NS); 2) 12% CP, low protein (LP); 3) 28% CP, moderate protein (MP); 4) 41% CP, high protein (HP). In Exp. 2 and 3, 16 ruminally cannulated steers were blocked by weight (avg wt = 332 kg, Exp. 2; 401 kg, Exp. 3) and assigned randomly to a 2 x 2 factorial arrangement of treatments. The treatments contrasted low (LP) and high (HP) levels of supplemental protein (.66 g CP/kg BW vs 1.32 g CP/kg BW) with low (LE) and high (HE) levels of supplemental ME (9.2 kcal/kg BW vs 18.4 kcal/kg BW). In Exp. 1, forage DMI as well as ruminal DM and indigestible ADF fill at 4 h postfeeding were greater (P less than .10) with the MP and HP steers than with control and LP steers. Total DM digestibility increased (P less than .10) for supplemented steers (35.5% for control vs 47.3 for supplemented steers); however, LP depressed (P less than .10) NDF digestibility. In Exp. 2, forage DMI, indigestible ADF flow and liquid flow were depressed (P less than .10) in LP-HE supplemented steers. In Exp. 3, HP steers had greater (P less than .10) forage DMI, indigestible ADF fill values (4 h postfeeding), liquid volume and tended (P = .11) to have greater ruminal DM fill (4 h postfeeding). In summary, increased levels of supplemental protein increased intake and utilization of dormant tallgrass-prairie forage (less than 3% CP). Increasing supplemental energy without adequate protein availability was associated with depressed intake and digestibility.  相似文献   

2.
The effects of including yeast culture (YC; Saccharomyces cerevisae plus growth medium; 5 x 10(9) organisms/g) in diets for ruminants was examined in two experiments. In Exp. 1, 32 multiparous Friesian dairy cows were fed between wk 7 to 12 of lactation one of four completely mixed diets based on either hay or straw plus rolled barley (mixed to give concentrate:forage ratios of either 50:50 or 60:40, respectively) with or without 10 g YC/d in a 2(3) factorial design. Supplementation with YC increased DM intake of the cows by a mean of 1.2 kg/d (P less than or equal to .062) and increased milk yield by 1.4 liters/d (corrected to 4% butterfat; P less than or equal to .05). There was an interaction (P less than .05) between diet composition and YC addition; effects of YC were greatest in diets containing 60:40 (concentrate:forage) ratio. In Exp. 2, three steers were fed a diet of 50% hay and 50% rolled barley (DM basis). Hay was available for the major part of the day but barley was fed in two meals/d. Addition of YC to the diet increased (P less than .05) ruminal pH for 4 h after the barley meal. This elevation in pH probably was due to a reduction (P less than or equal to .01) in the concentration of L-lactate in the ruminal liquor of steers given YC (1.43 vs 3.55 mM; P less than or equal to .01). Peak ruminal L-lactate concentration (7.75 mM) in the controls coincided with time of minimum pH values (2 h after the meal of barley); this peak was absent in steers given YC. YC had no effect on the concentration of VFA in ruminal liquor, but the ratio of acetate to propionate was reduced (P less than or equal to .01) from 3.3:1 to 2.8:1 in steers given YC. The extent of DM degradation of hay incubated in the rumen of steers fed the hay and rolled barley diet was increased (P less than .05) in the presence of YC at 12 h of incubation, but degradation was similar in all treatment groups after 24 h of incubation. Presence of yeast culture in the rumen had effects on ruminal stoichiometry. An increased rate of forage degradation may have increased forage intake and productivity of these dairy cows.  相似文献   

3.
Three experiments were conducted to study the effects of lasalocid level on performance, intake, digestibility, ruminal fermentation and fluid flow of beef cattle grazing dormant, tallgrass prairie. In Exp. 1, 120 pregnant, mature beef cows of primarily Hereford breeding (avg wt = 471 kg) were randomly assigned to received 0, 100, 200 or 300 mg lasalocid X head-1 X d-1 in 1.82 kg supplement. Weight changes at 30, 60 or 90 d, condition score change and calf birth weight were not affected (P greater than .10) by lasalocid level. In Exp. 2, estimates of intake and digestibility were obtained with 40 pregnant, mature Hereford cows (avg wt = 474 kg) and 12 esophageal-cannulated, Hereford X Angus steers (avg wt = 225 kg), using Yb and indigestible acid detergent fiber as markers for fecal output and digestibility, respectively. Levels of lasalocid provided to cows and steers were similar, on a body weight (BW) basis, to those in Exp. 1 and corresponded to approximately 0, .22, .44 or .66 mg lasalocid/kg BW. Total diet and forage organic matter digestibility for beef cows decreased (P less than .01) at the .22 mg/kg BW level, but increased at the .44 and .66 mg/kg BW levels. Organic matter intake was not influenced (P greater than .10) by lasalocid addition. In Exp. 3, 16 ruminal-cannulated, Hereford X Angus steers (avg wt = 227 kg) were given the same lasalocid dosages per kg BW as in Exp. 2, and were used to study the effects of lasalocid on ruminal fermentation and fluid flow characteristics.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

4.
Studies were conducted to determine the effects of lysocellin on growth performance and metabolism of steers fed forage-based diets. Treatments in all experiments consisted of 1) control, 2) 100 mg lysocellin/d, 3) 200 mg lysocellin/d and 4) 200 mg monensin/d. In each of two 90-d performance studies, 24 Hereford steers were individually fed greenchop (fungus-free tall fescue and Coastal and Tifton-44 bermudagrass) ad libitum and .91 kg/d of a corn-trace mineral salt supplement. In Exp. 1, tall fescue was fed from d 1 to 45 and bermudagrass from d 46 to 90. Bermudagrass was offered during d 1 to 45 and tall fescue during d 46 to 90 in Exp. 2. Lysocellin improved gain (Exp. 1, P less than .01) and feed conversion (Exp. 1 and 2 combined, P less than .05), decreased total VFA concentrations (P less than .05), increased molar proportions of propionate, isobutyrate and isovalerate (P less than .01), decreased molar proportions of acetate and butyrate (P less than .01) and lowered acetate:propionate (P less than .01). Two metabolism studies involving a total of 16 Hereford steers were conducted. Steers were fed tall fescue greenchop and .91 kg/d supplement for a 34-d adjustment period followed by a 5-d total collection period. Lysocellin increased N digestibility (P less than .01) and N retention (P less than .06) but did not (P greater than .05) affect DM, NDF or ADF digestibility. Data indicate that lysocellin results in major alterations in ruminal fermentation and can increase growth performance and N retention in steers fed forage-based diets.  相似文献   

5.
Two experiments were conducted to determine effects of feeding corncobs of various mean particle size (MPS) on ruminal, digestive and metabolic characteristics of early-weaned lambs fed pelleted 74.9% concentrate:25.1% corncob diets. The MPS of corncobs in diets was 6.5, 5.4, 1.4 and .8 mm, respectively. As particle size decreased, percentage starch decreased and percentage neutral detergent fiber (NDF), acid detergent fiber (ADF) and cellulose increased. In Exp. 1, 28 crossbred rams (seven/treatment, avg initial wt, 15.3 kg) were used in a randomized complete-block design. In Exp. 2, lambs from Exp. 1 were re-weighed (avg initial wt, 16.8 kg) and fed the same diets as in Exp. 1. In Exp. 1 and 2, lambs ingested dry matter (DM) equal to 2.68 and 3.74% of body weight, respectively. In Exp. 1, apparent DM digestibility was unaffected by corncob MPS; however, in Exp. 2, DM digestibility was highest (68.8%) for lambs fed the 6.5-mm diet and lowest (63.8%) for those fed the .8-mm diet. Apparent starch digestibility was high (greater than 98.8%) in both experiments. Neutral detergent fiber and ADF digestibilities were highest for lambs fed the 1.4-mm diet (50.5 and 43.6%, Exp. 1; 39.6 and 28.9%, Exp. 2). A dramatic increase (6.8 to 39.1%) in acid detergent lignin (ADL) digestibility was observed in Exp. 1 as corncob MPS decreased. In Exp. 2, ADL digestibilities were similar for lambs fed the 6.5-, 5.4- or 1.4-mm diets (avg value, 5.9%) and highest for those fed the .8-mm diet (29.7%). Nitrogen metabolism was unaffected by corncob MPS. In Exp. 1, digestible energy intake, corrected for urinary losses, did not differ among treatments but in Exp. 2, lambs consuming the 6.5-mm diet had higher corrected digestibility energy intakes (1,926.6 kcal/d) than did those fed other diets (avg, 1,832.4 kcal/d). Ruminal pH sampled 4 h post-feeding was highest for lambs consuming the 6.5-mm diet (6.25) in Exp. 1 and the 1.4-mm diet (5.89) in Exp. 2. Lowest ruminal pH (5.30 and 5.36, respectively) was for lambs consuming the .8-mm diet in Exp. 1 and the 5.4-mm diet in Exp. 2. Ruminal lactate concentrations were variable within and among treatments. Total ruminal volatile fatty acid (VFA) concentrations were similar across treatments but in Exp. 2, there was a shift in molar proportions from acetate to propionate as corncob MPS decreased.(ABSTRACT TRUNCATED AT 400 WORDS)  相似文献   

6.
Two trials evaluated the effects of a monensin ruminal delivery device (MRDD) on steers grazing winter wheat pasture. In Trial 1, 60 Hereford steers (initial wt 238.5 kg) grazed a 21.9-ha paddock of Vona-variety winter wheat for 112 d. Steers were assigned to either MRDD or control (C) treatments in a randomized complete block design. In Trial 2, eight ruminally cannulated steers (avg wt 234.4 kg) grazed a 2.4-ha paddock of Vona-variety wheat and were assigned randomly to either MRDD or C treatments. Three 11-d collection periods were conducted during early February, early March and early April. Chromic oxide was dosed to determine fecal output, and ruminal samples were collected on d 6 of each period. Nylon bags containing ground wheat forage were incubated ruminally beginning on d 8. In Trial 1, steers with MRDD tended (P less than .11) to gain more weight than C steers (.44 vs .38 kg/d). In Trial 2, wheat forage intake, in situ DM disappearance, ruminal pH, ruminal ammonia concentrations and ruminal proportions of acetate and total VFA concentrations were not affected by treatment. Ruminal proportions of propionate were increased (P less than .05) slightly by MRDD (20.3 and 19.2 mol/100 mol for MRDD and C, respectively). Butyrate proportions in ruminal samples were decreased (P less than .05) by MRDD during March but not in other sampling periods. Ruminal fluid chlorophyll concentration was less (P less than .05) for MRDD-treated vs C steers during early March but was greater (P less than .10) for MRDD-treated steers during early April. The MRDD shows promise as a method of supplying monensin to cattle grazing winter wheat forage.  相似文献   

7.
Four experiments were conducted to evaluate the effects of dietary volatile fatty acids (VFA) on nutrient digestibility, ruminal characteristics, and growth performance by finishing steers. Diets were based on sorghum grain in Exp. 1 and 2, and corn in Exp. 3 and 4. Experiment 1 was a digestibility study with eight steers used in two 4 x 4 latin square arrangements of steers and diets. Diets in the first square were all-concentrate (C) with 1 or 2% acetate (A) or 2% propionate (P), and diets in the second square were 20% rice straw (RS) with 1 or 2% A or 2% P. In Exp. 2, steers (four per treatment) were individually fed C or RS basal diets with 1.72% A or 5.00% P added to the C diet, and 1.14% A or 3.33% P added to the RS diet based on expected intake. In Exp. 3 and 4, C diets were diluted with 1.65 or 0.55% A, respectively. These diets were further diluted with 20% rice hulls (RH) in Exp. 3, and 20% RH and 20% RS in Exp. 4. The C diet was also diluted with 20% RS in Exp. 3 and considered a positive control. Adding A, P, or RS to C diet based on sorghum grain had no effect (P>0.10) on energy digestibility or N retention. In Exp. 2, P reduced (P<0.05) DMI and ADG, compared with A. Acetate improved (P<0.05) DMI of C and RH diet in Exp. 3, but only the RH diet in Exp. 4. Likewise ADG and, in most cases, carcass weights were improved (P<0.05) by A in C and RH diets, but not in RS diets. Dietary P reduced (P<0.05) ruminal acetate levels and increased (P<0.05) ruminal propionate in Exp. 2. Acetate may have potential to improve DMI and ADG of steers consuming diets containing all-concentrate or poorly digestible roughage.  相似文献   

8.
Six beef steers (British x Brahman) cannulated at the rumen, duodenum and ileum (avg wt 334 kg) and three mature steers (British x British) cannulated at the esophagus were used in a replicated 3 x 3 latin square design and fed no supplement (C), .5 kg soybean meal (SBM) or .5 kg steam-flaked sorghum grain (SFS).head-1.d-1 (DM basis) while grazing blue grama rangeland. Periods of the latin square included a minimum of 14 d for adaptation and 11 d for esophageal masticate collection and digesta sampling. In September, October and November, respectively, forage collected by esophageally cannulated steers averaged 74.5, 88.8 and 71.0% grasses; 2.06, 1.53 and 1.77% N and 68.3, (P greater than .10) by treatment, but total N intake was greater (P less than .05) for SBM vs C and SFS treatments. No differences (P greater than .10) were detected among treatments in OM, NDF, ADF and N digestibilities in the rumen, small intestine or hindgut, but total tract OM digestibility was greater (P less than .10) for SBM and SFS than for C, and total tract N digestibility was greater (P less than .10) for SBM than for C or SFS. Duodenal ammonia N flow was greater (P less than .05) when SBM was fed that when SFS and C were fed, but microbial N and non-ammonia, non-microbial N flows and microbial efficiency were not altered by treatment. Likewise, ileal N flow was not affected (P greater than .10) by treatment. Particulate passage rate, gastrointestinal mean retention time, forage in vitro OM disappearance and in situ rate of forage NDF digestion also were not affected (P greater than .10) by treatments. Ruminal fluid volume was greater (P less than .05) for SFS vs SBM and C treatments, but no differences were noted in fluid dilution rate. Ruminal fluid ammonia concentration was greater (P less than .05) when SBM was fed than when SFS and C were fed (13.5, 9.9 and 8.7 mg/dl, respectively), whereas pH and total VFA concentrations were not different (P greater than .10). Proportion of acetate in ruminal fluid was less (P less than .10) for SBM and SFS than for C. Small amounts of supplemental SBM and SFS had little effect on forage intake, ruminal fermentation and site of digestion but both increased total tract OM digestion in steers grazing blue grama rangeland.  相似文献   

9.
Objectives of this research were to evaluate effects of increasing level of barley supplementation on forage intake, digestibility, and ruminal fermentation in beef steers fed medium-quality forage. Four crossbred ruminally cannulated steers (average initial BW = 200 +/- 10 kg) were used in a 4 x 4 Latin square design. Chopped (5 cm) grass hay (10% CP) was offered ad libitum with one of four supplements. Supplements included 0, 0.8, 1.6, or 2.4 kg of barley (DM basis) and were fed in two equal portions at 0700 and 1600. Supplements were fed at levels to provide for equal intake of supplemental protein with the addition of soybean meal. Forage intake (kg and g/kg BW) decreased linearly (P < 0.01), and total intake increased linearly (P < 0.03) with increasing level of barley supplementation. Digestible OM intake (g/kg BW) increased linearly (P < 0.01) with increasing level of barley supplementation; however, the majority of this response was observed with 0.8 kg of barley supplementation. Treatments had only minor effects on ruminal pH, with decreases occurring at 15 h after feeding in steers receiving 2.4 kg of barley supplementation. Total-tract digestibility of DM, OM, NDF, and CP were increased (P < 0.04) with barley supplementation; however, ADF digestibility was decreased by 1.6 and 2.4 kg of barley supplementation compared with controls. Ruminal ammonia concentrations decreased linearly (P < 0.01) at 1 through 15 h after feeding. Total ruminal VFA concentrations were not altered by dietary treatments. Ruminal proportions of acetate and butyrate decreased (P < 0.10) in response to supplementation. Rate, lag, and extent (72 h) of in situ forage degradability were unaffected by treatment. Generally, these data are interpreted to indicate that increasing levels of barley supplementation decrease forage intake, increase DM, OM, and NDF digestibility, and indicate alteration of the ruminal environment and fermentation patterns.  相似文献   

10.
Thirty-two mature, pregnant beef cows (avg age 8 yr, body wt 550 kg) grazing oat pastures were used to determine the effects of lasalocid on serum mineral and ruminal fluid volatile fatty acid (VFA) concentrations. Cows were blocked by breed type and assigned randomly to eight, 2-ha pastures containing oat forage from December 17, 1985 to May 6, 1986. The cows were fed .25 kg of ground corn with or without 200 mg of lasalocid daily. Based upon analyses of forage mineral composition (.53, .25, .14, .3 and 2.5% of dry matter for Ca, P, Mg, Na and K, respectively), all macrominerals except Mg were in sufficient quantities to meet the requirement for lactating cows. Lasalocid supplementation did not affect serum Mg, K or Zn concentrations (P greater than .05). During the April sampling, serum Ca was higher (P less than .05) and serum Na was lower (P less than .05) in cows supplemented with lasalocid compared with controls. The molar proportion of acetate was decreased (P less than .05) in ruminal fluid of cows fed lasalocid by d 56 (February 11); the molar proportions of acetate and butyrate were decreased (P less than .05), which decreased (P less than .05) the acetate: propionate ratio by d 84 (March 11) of supplementation. This study suggests that lasalocid is effective in altering ruminal VFA and serum mineral concentrations in cows grazing small grain forages.  相似文献   

11.
Two trials were conducted to determine the effects of transport stress and pre-transport diet on N losses, blood chemistry and ruminal variables of steers. In each trial, 16 crossbred steers (261 kg avg weight) were assigned to four groups in a 2 X 2 factorial arrangement of treatments. Treatments consisted of either transported or non-transported groups and two pre-transport dietary regimens (alfalfa hay or a 50% concentrate diet fed for 3 d before fasting). The alfalfa hay contained 14.6% crude protein (CP) and had a calculated metabolizable energy (ME) content of 1.92 mcal/kg. The 50% concentrate diet contained 15.5% CP and had a calculated ME content of 2.23 mcal/kg. Steers in the transport group were transported for 13 h in trial 1 and 46 h in trial 2, while the remaining steers were not transported. Both groups were withheld from feed and water during the observation periods. Urine and feces were collected and blood samples were obtained at intervals during the observation periods. Transport increased (P less than .05) urinary and total N excretion and nonevaporative water losses compared with fasting alone. Calves fed the 50% concentrate diet had lower N and nonevaporative water losses than calves fed hay, probably due to lower pre-fast N and water intakes. Ruminal total volatile fatty acid (VFA) concentrations and molar proportions of propionate and butyrate declined (P less than .05) with time in all groups, while molar proportions of acetate and minor VFA increased. Results of these trials indicate transportation stress can cause a significant increase in total N excretion and nonevaporative water loss compared with feed and water deprivation alone. Losses of N and water were lower in calves fed a 50% concentrate diet rather than hay for 3 d before the fasting period, probably because of lower pre-fast N and water intakes.  相似文献   

12.
The objective of Exp. 1 was to compare the effects of virginiamycin (VM; 0, 175, or 250 mg x animal(-1) x d(-1)) and monensin/tylosin (MT; 250/ 90 mg x animal(-1) x d(-1)) on ruminal fermentation products and microbial populations in cattle during adaptation to an all-concentrate diet. Four ruminally cannulated, Holstein steers were used in a 4x4 Williams square design with 21-d periods. Steers were stepped up to an all-concentrate diet fed at 2.5% of BW once daily. Ruminal pH, protozoal counts, and NH3-N and VFA concentrations generally were unaffected by VM or MT. Mean counts of Lactobacillus and Streptococcus bovis were lower (P<.05) for VM-treated compared with control or MT-treated steers. Both VM and MT prevented the increase in Fusobacterium necrophorum counts associated with increasing intake of the high-concentrate diet observed in the control. The objective of Exp. 2 was to compare the effects of VM and MT on ruminal pH, L(+) lactate and VFA concentrations, and F. necrophorum numbers during carbohydrate overload. Six ruminally cannulated Holstein steers were assigned randomly to either the control, VM (175 mg/d), or MT (250 + 90 mg/d) treatments. Acidosis was induced with intraruminal administration of a slurry of ground corn and corn starch. The VM and MT premixes were added directly to the slurry before administration. Carbohydrate challenge induced acute ruminal acidosis (pH was 4.36 and L (+) lactate was 19.4 mM) in controls by 36 h. Compared with the controls, steers receiving VM or MT had higher (P<.05) ruminal pH, and the VM group had a lower (P<.05) L (+) lactate concentration. Fusobacterium necrophorum numbers initially increased in VM- and MT-administered steers. In the control steers, F. necrophorum was undetectable by 36 h. Virginiamycin seemed to control the growth of ruminal lactic acid-producing bacteria and, therefore, has the potential to moderate ruminal fermentation in situations that could lead to rapid production of lactic acid.  相似文献   

13.
Three lamb metabolism experiments were conducted to investigate the effects of chronic administration of the novel urease inhibitor N (n-butyl) thiophosphoric triamide (NBPT) on ruminal N metabolism, fermentation, and N balance. In Exp. 1, ruminally cannulated wethers (n = 28; 45.0 +/- .9 kg) were administered one of seven doses of NBPT (0 [control], .125, .25, .5, 1, 2, or 4 g of NBPT daily) and fed a common cracked corn/cottonseed hull-based diet twice daily containing 2% urea at 2.5% of initial BW for the duration of the 15-d experiment. Overall, NBPT decreased (linear P < .0001; quadratic P < .001) ruminal urease activity, resulting in linear increases (P < .0001) in ruminal urea and decreases in ruminal NH3 N concentrations. However, the detection of an NBPT x day interaction (d 2 vs 15; P < .01) indicated that this depression in urea degradation diminished as the experiment progressed. Increasing NBPT linearly decreased (P < .01) total VFA concentrations on d 2 of the experiment, but it had no effect (P > .10) on d 15. Increasing NBPT had no effect (P > .10) on DM or ADF digestibilities, but it linearly decreased (P < .01) N digestibility. Supplementing NBPT produced a linear increase (P < .05) in urinary N excretion and a linear decrease (P < .01) in N retention. In Exp. 2, ruminally cannulated wethers (n = 30; 46.8 +/- .6 kg) were fed one of two basal diets (2.0 vs 1.1% dietary urea) at 2.5% of initial BW and dosed with either 0 (control), .25, or 2 g of NBPT daily for the duration of the 15-d experiment. There were no NBPT x dietary urea interactions (P > .10) for Exp. 2. Increasing NBPT depressed (linear and quadratic P < .0001) ruminal urease activity, producing linear (P < .0001) increases in urea N and linear decreases in NH3 N in the rumen. As in Exp. 1, an NBPT x day interaction (P < .05) was noted for urea, NH3 N, and total VFA concentrations; the maximum response to NBPT occurred on d 2 but diminished by d 15 of the experiment. Administration of NBPT did not influence (P > .10) DM, ADF, or N digestibilities in Exp. 2. In Exp. 3, wether lambs (n = 30; 26.4 +/- .7 kg) were subjected to the same treatment regimen as in Exp. 2 for a 14-d N balance experiment. Although several NBPT x dietary urea interactions (P < .05) were noted, increasing NBPT did not affect (P > .10) N digestibility. Administration of NBPT quadratically increased (P < .10) urinary N excretion, producing a linear decrease (P < .05) in N retention. These results suggest that although NBPT is capable of inhibiting ruminal urease short-term, the ruminal microflora may be capable of adapting to chronic NBPT administration, thereby limiting its practical use in improving the utilization of dietary urea.  相似文献   

14.
An in situ study (Exp. 1) using 4 ruminally cannulated steers (343 ± 11 kg of BW) in a completely randomized design was used to compare ruminal degradation characteristics of low-quality cool-season (C3; Kentucky bluegrass straw; Poa pratensis; 6.3% CP; DM basis) and warm-season (C4; tallgrass prairie; 5.7% CP; DM basis) forage. Four ruminally cannulated steers (252 ± 8 kg of BW; Exp. 2) and 4 wethers (38 ± 1 kg of BW; Exp. 3) were used in two 2 × 2 factorial arrangements of treatments to determine the influence of supplemental CP (CPSupp; soybean meal; 0.09 and 0.19% of BW, CP basis, for steers and lambs, respectively) on nutrient intake and digestion of C3 and C4 forages. Steers and wethers were allotted to separate 4 × 4 Latin squares that ran simultaneously with 20-d periods. In Exp. 1, C3 had a greater A fraction (fraction of total pool disappearing at a rate too rapid to measure) and effective degradability of DM and NDF compared with C4 (P < 0.01). In addition, C3 had a greater (P < 0.01) A fraction and effective degradability of N, whereas the C fraction (fraction of total pool unavailable in the rumen) was less (P < 0.01) than those for C4. Consequently, RDP accounted for 84.7% of total CP in C3 as compared with 66% for C4 (P < 0.01). In Exp. 2, a CPSupp × forage interaction (P < 0.01) was noted for forage and total DMI, with CPSupp increasing intake of C4 by 47% and intake of C3 forage by only 7%. Dry matter digestibility responded similarly, with a CPSupp × forage interaction (P = 0.05; CPSupp increased digestibility by 21% with C4 and by 9% with C3 forage). In addition, CPSupp × forage interactions were noted for ruminal liquid retention time (P = 0.02; CPSupp decreased retention by 3.6 h with C4 and by only 0.6 h with C3 forage) and particulate passage rate (P = 0.02; CPSupp increased passage by 46% with C4 and by 10% with C3 forage). As in Exp. 2, a CPSupp × forage interaction (P = 0.01; CPSupp increased digestibility by 18% with C4 and by 7% with C3 forage) was observed with DM digestibility in Exp. 3. In contrast, only N balance (P < 0.01) and N digestibility (P < 0.01) were affected by CPSupp. These data suggest that intake and digestion of low-quality C3 and C4 forages by ruminants are not similar and, more important, that the physiological response of ruminants to protein supplementation of low-quality forage is dependent on forage type.  相似文献   

15.
Two experiments were conducted to determine the influence of dietary roughage concentration and feed intake on finishing steer performance and ruminal metabolism. In Exp. 1, 126 steers (334 kg) were used in a completely randomized design and fed (120 d) diets of steam-rolled wheat without roughage or containing 5, 10 or 15% roughage (50% alfalfa hay:50% corn silage). Steers fed 5 or 10% roughage gained faster (quadratic, P less than .05) and were more efficient (quadratic, P less than .05) than steers fed 15% or no roughage. In Exp. 2, six ruminally cannulated steers (447 kg) were used in a 6 x 6 latin square design and fed (twice daily) diets of steam-rolled wheat without roughage or containing 5 or 15% alfalfa hay at twice or three times NE required for maintenance. Increasing dietary roughage increased (linear, P less than .01) ruminal liquid passage 38%, indigestible ADF passage 63%, Yb-labeled wheat passage 75% and fiber fill 31%. The rate of in situ starch digestion tended to increase (linear, P = .16), and ruminal VFA concentration was 40 mM higher (P less than .01) at 4 h after feeding with increased roughage. Increased feed intake increased (P less than .05) ruminal starch fill, fiber fill, liquid fill and liquid passage 23%, Yb-labeled wheat passage 50% and Dry-labeled hay passage 20%. It reduced protozoa five- to sixfold (P less than .01) but doubled total bacterial counts (P less than .01). Ruminal NH3N was lower (P less than .01) and total VFA concentration was 50 mM higher (P less than .01) at 4 h after feeding. The acetate:propionate ratio was reduced from 2.3 to 1.3 (P less than .01) with increased intake. Adding roughage to a steam-rolled wheat diet increased passage and tended to increase rate of starch digestion; increased feed intake with its associated effects on ruminal fill and passage dramatically shifted the microbial population and fermentation end products.  相似文献   

16.
Lambs (29 +/- 2.5 kg) were fed three diets at various intakes to determine whether diet composition or level of intake was reflected in changes in diet digestibility or ruminal fluid characteristics. In Exp. 1, a 90% concentrated, pelleted diet or a whole shelled corn diet with a pelleted protein supplement was fed at three levels of intake: ad libitum and 92.5 and 85% of ad libitum (n = 15). Exp. 2 compared the 90% concentrate diet with diets in which the energy density was diluted to 55 or 72.5% concentrate by including alfalfa hay as a possible method of restricting energy intake (n = 6). Lambs were adapted to diets for 13 d; feces were collected for 6 d and ruminal fluid was collected 0, 3, and 6 h after feeding on the day following fecal collection. Restricting intake in Exp. 1 did not affect DM digestibility or digestibility of CP or starch. Digestibility of ADF was increased (P less than .10) by restricting intake. Ruminal fluid pH, ammonia concentration and VFA concentrations were affected little by either restricted intake level. Digestibility of DM was 4% higher (P less than .001) and starch 5% higher (P less than .001) for the whole shelled corn diet than for the pelleted, high-concentrate diet. Ruminal pH of lambs fed the whole shelled corn diet was higher and fluctuated less than the ruminal pH of lambs fed the high-concentrate, pelleted diet. In Exp. 2, diet digestibility was reduced (P less than .01) and ruminal pH was increased (P less than .002) by addition of hay. Restricted feeding of lambs did not seem to increase diet digestibility or alter ruminal conditions.  相似文献   

17.
Two experiments were conducted to determine how varying the proportion of wheat (W) and high-moisture sorghum grain (SG) in 80% grain dies would affect ruminal fermentation, liquid and solid flow, site and extent of digestion (Exp. 1) and feeding performance of cattle (Exp. 2). In Exp. 1, three ruminal, duodenal and ileal cannulated steers (average weight 295 kg), fed at 1.54% of body weight, were used in a six-period crossover design. Treatments were: W, 50W:50SG (W:SG) and SG. Increasing wheat level decreased ruminal pH, molar proportion of acetate, and acetate:propionate ratio (P less than .05) and increased (P less than .05) L-lactate concentration, molar proportions of propionate and valerate and total volatile fatty acid concentration. Ruminal liquid dilution and outflow rates were faster (P less than .05) and retention time was shorter (P less than .05) for the W diet. Duodenal and ileal liquid flow increased (P less than .05), and solid flow decreased (P less than .05), as dietary level of wheat increased. Apparent ruminal digestion (% of intake) of dry matter (DM) and organic matter (OM) was greater (P less than .01) with the wheat-containing diets. Intestinal DM and OM digestion (percent of intake) was higher (P less than .05) with the SG and W:SG diets. Ruminal, small intestine, large intestine and total tract starch digestion (percent of intake) was 93.5, 5.6, .7, 99.8, 71.5, 20.4, 5.7, 97.6; and 48.0, 32.5, 10.5, 91.0 with the W, W:SG and SG diets, respectively. In Exp. 2, group-fed (24 pens) steers (avg initial weight 341 kg) were fed ad libitum once daily for 121 d. Treatments were: W, 67W:33SG, 33W:67SG and SG. Rates of gain (kg/d) with the W (1.32), 67W:37SG (1.33) and 33W:67SG (1.30) diets were similar (P greater than .05), but faster (P less than .05) than those with the SG diet (1.16). Feed intake was lower (P less than .01) with the W and 67W:33SG diets, but the wheat-containing diets were utilized more efficiently (P less than .01). Increasing the proportion of wheat in sorghum grain feedlot diets improved cattle performance by optimizing ruminal and post-ruminal digestion.  相似文献   

18.
Two finishing trials and a metabolism trial were conducted to evaluate the effect of forage source and particle size in dry-rolled corn finishing diets. In Exp. 1, 224 crossbred yearling steers (BW = 342+/-11 kg) were used in a randomized complete block design consisting of seven treatments. Treatments were an all-concentrate diet or diets containing equal NDF levels provided by alfalfa hay or wheat straw (three treatments each) with each forage source ground to pass through a .95-, 7.6-, or 12.7-cm screen. Steers fed diets containing forage had greater (P < .05) DMI than steers fed an all-concentrate diet. Steers fed alfalfa diets gained faster (P < .05) with a greater (P < .05) concentrate efficiency than steers fed either all-concentrate or straw diets. In Exp. 2, 120 crossbred yearling steers (BW = 307+/-2 kg) were used in a completely randomized design and fed dry-rolled corn diets containing 10% alfalfa ground to pass through either a .95- or 7.6-cm screen. Alfalfa particle size had no effect on performance or carcass measurements. In Exp. 3, six ruminally fistulated steers (BW = 508+/-34 kg) were used in a 6 x 6 Latin square design and fed an all-concentrate diet or diets containing equal NDF levels provided by alfalfa hay, wheat straw, or ground corncobs with alfalfa and straw ground to pass through either a 2.54- or 12.7-cm screen. Steers fed straw diets spent more time (P < .10) chewing than those receiving the other diets. In conclusion, forage particle size had no effect on finishing cattle performance or ruminal metabolism data. However, cattle consuming different forage sources in dry-rolled corn finishing diets may not respond similarly in animal performance.  相似文献   

19.
Five ruminally fistulated 3-yr-old mature Holstein steers (average BW 691+/-23 kg) were used in a 5 x 5 Latin square experiment with a 2 x 2 + 1 fact orial arrangement of treatments. Effects of protein concentration and protein source on nutrient digestibility, excretion of DM and fecal N, ruminal fluid volume and dilution rate, ruminal characteristics, and in situ DM disappearance of whole shelled corn, ground corn, and orchardgrass hay were measured in steers limit-fed high-concentrate diets at 1.5% of BW. A negative control basal diet (NC; 9% CP) was supplemented to achieve either 11 or 14% CP; supplemental CP was either from soybean meal (11 and 14% SBM) or a 50:50 ratio of CP from urea and soybean meal (11 and 14% U). Dry matter and OM digestibilities were 5% greater (P < .07) for steers fed the SBM diets than for those fed the U diets. Starch digestibility did not differ (P > .10) among steers fed any of the diets. Nitrogen source did not affect (P > .10) apparent N digestibility or fecal N excretion; however, steers fed the NC diet had the lowest (P < .10) apparent N digestibility compared with those fed all other diets. Ruminal fluid volume was lower (P < .06) when steers were fed the NC diet compared with all other diets; there were no differences (P > .74) among diets for ruminal fluid dilution rate. In general, ruminal ammonia N and VFA molar proportions were not affected by protein source or concentration. Although CP concentration affected (P < .06) in situ DM disappearance of ground corn, CP concentration did not (P > .48) affect total tract digestion of DM or OM. This indicates that CP concentration may have affected site of digestion, but not extent of digestion. When mature ruminants were limit-fed a corn-based diet to meet primarily a maintenance function, protein source and concentration had little effect on measures of nutrient digestion.  相似文献   

20.
Response to monensin in cattle during subacute acidosis   总被引：1，自引：0，他引：1  
A steer metabolism study was conducted to measure changes in ruminal and blood components in response to monensin level following an abrupt switch from forage to a concentrate diet. Six ruminal-cannulated crossbred steers (373 kg) were fed either 0, 150 or 300 mg monensin per head daily in a replicated 3 X 3 Latin-square design. In all treatments, ruminal pH declined to a low of 5.4 to 5.6 12 h post-feeding, suggesting steers experienced subacute acidosis. Also in the first 12 h post-feeding, all treatments exhibited nearly a twofold increase in total ruminal volatile fatty acid (VFA) concentrations, while peak ruminal lactate concentrations ranged from .86 to 1.50 mM. During the entire 48-h period, there were no significant treatment differences in blood pH, HCO3- or ruminal lactate, although there was a trend of higher ruminal and blood lactate associated with increased level of monensin supplementation. Feeding higher levels of monensin resulted in higher pH and propionate with lower acetate and butyrate concentrations. Increasing the level of monensin fed resulted in reduced (P less than .01) total ruminal VFA concentrations. Ruminal pH was more highly correlated to total ruminal VFA concentrations (r = -.69, P less than .01) than lactate concentrations (r = -.14, P less than .10). Results from this study indicate the significance of total ruminal organic acid concentration rather than ruminal lactate concentration during subacute acidosis. Monensin maintained a higher ruminal pH by reducing concentrations of VFA.  相似文献   

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