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抑制素主要是由雄性动物的睾丸支持细胞和雌性动物的卵泡颗粒细胞所分泌的一种糖蛋白。它由2个二硫键将1个α亚基和1个β亚基连接而成,分子量3.1万～10万。不同种属动物的抑制素,其分子结构极为相似。公畜抑制素主要是选择性地抑制FSH的分泌,并与睾酮具有协同作用。 相似文献
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主要介绍了激活素对生殖的作用,激活素亚基βA和βB及其受体在生殖系统中的表达分布,以及激活素的作用机制。激活素A对动物生殖具有刺激作用,但这种作用具有时间的特异性;激活素的主要作用除了受激活素受体、还受卵泡抑素和抑制素等的调节作用。 相似文献
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母鸡抑制素的研究进展 总被引：4，自引：0，他引：4
前言哺乳动物抑制素（ｉｎｈｉｂｉｎ）是一种性腺糖蛋白，由α亚基和βＡ、βＢ亚基组成的异二聚体：α亚基和βＡ亚基构成抑制素Ａ；α亚基和βＢ构成抑制素Ｂ；βＡ和βＢ亚基则组成激活素（ａｃｔｉｖｉｎ）二聚体。卵泡液和外周还存在游离的α前体物，在母鸡，抑制素在卵巢、肾上腺、脑、肌肉等组织中表达。由于抑制素结构的复杂性，至今尚没有办法精确的测定鸡外周抑制素的量，给研究抑制素的生物学活性带来困难。但采用测定各组织中α亚基和βＡ、βＢ亚基ｍＲＮＡ的表达量，可以反映抑制素的来源、分布及其作用。本文总结了近年来母… 相似文献
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抑制素及其对动物繁殖的影响 总被引：7，自引：0，他引：7
抑制素是一种性腺分泌的糖蛋白激素 ,主要由雄性睾丸支持细胞和雌性卵颗粒细胞分泌。抑制素对于动物生殖机能具有重要调节作用。本文将对其分子结构、特性、生成转运、对生殖活动的调节及其免疫在动物繁殖上的运用加以论述。1 抑制素的分子结构和特性抑制素 (Ｉｎｈｉｂｉｎ ,ＩＢ)是一种大分子糖蛋白 ,由α和β两个不同的亚单位通过二硫键偶联而成[1] 。分子量变化范围很大 ,约 2 0 0 0～ 2 0 0 0 0 0。不同种属动物抑制素氨基酸序列差异很小。α单位上有糖基化位点 ,β单位有Ａ、Ｂ两种类型。因此 ,抑制素有两种形式 ,即抑制素Ａ(αβＡ)… 相似文献
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抑制素是一种主要由动物性腺分泌的异二聚体蛋白激素 ,它通过选择性抑制垂体促性腺激素 (主要是 FSH)的分泌 ,进而影响动物的生殖活动。而利用抑制素对动物主动或被动免疫 ,可增加排卵率 ,提高繁殖力的独特功能越来越受到人们的重视。本文就近年来有关抑制素免疫对动物生殖功能影响的研究作一综述。1 抑制素的类型在抑制素免疫研究中 ,有 3种形式的免疫原 ,即天然抑制素制剂、合成抑制素肽段和重组型抑制素。1 .1 天然抑制素 主要有牛抑制素 ( b MPI)和羊抑制素 ( o MPI) ,它是用一种单克隆抗体 2 56 H(其具有对牛 3 2 KD抑制素特异… 相似文献
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何兰花 《广东畜牧兽医科技》2003,28(5):21-23
抑制素(Inh ib in,IB)是一种由雌性卵泡颗粒细胞和雄性睾丸支持细胞分泌的糖蛋白激素,由α和β亚基组成的二聚体,其主要生理作用是特异性地调控垂体FSH(促卵泡素)的分泌。自1932年Macullagh提出这个概念以来,动物抑制素的研究日益受到人们的重视。目前对哺乳动物抑制素合成的部位、分子结构及生理作用已经有了深入的认识,利用蛋白质分离及分子原位杂交技术已人工合成了牛、羊、人的重组抑制素,固相合成技术已成功地合成了有功能的α片段N端 相似文献
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Ma X Zhang H Weng J Sheng X Lu L Hu X Liu S Xu M Weng Q Watanabe G Taya K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(9):1199-1205
The objective of this study was to investigate the cellular immunolocalization of inhibin a and inhibin/activin (β(A) and β(B)) subunits in the muskrat testes and scented glands during the breeding season. Inhibin α and inhibin/activin (β(A) and β(B)) subunits were expressed in Sertoli cells and Leydig cells of testes and glandular cells of scented glands, respectively. Also, positive signals of inhibin α and inhibin/activin (β(A) and β(B)) subunits by Western blotting were both observed in testicular and scented glandular tissues. These results suggested that the testes and scented glands of the muskrats had the ability to synthesize inhibins and activins and that activins and inhibins might play an important role in testicular and scented glandular function in muskrats. 相似文献
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Weng Q Wang H S Medan M Jin W Xia G Watanabe G Taya K 《The Journal of reproduction and development》2006,52(5):607-616
In the present study, the expression of inhibin/activin subunits in the mouse ovary from 13 days post-coitus (dpc) to 30 days postpartum (dpp) was investigated. Circulating FSH, LH, inhibin A, and inhibin B in neonatal to 30 dpp ovaries were measured. Inhibin/activin subunits (alpha, beta(A), beta(B) ) were weakly stained in 13 dpc ovarian stromal cells and increased with age. Inhibin alpha subunit was immunolocalized in follicular granulosa cells at each developmental stage. In 30 dpp ovaries, several large antral follicles were strongly stained for inhibin alpha subunit. Inhibin beta(A) subunit was weakly immunolocalized in granulosa cells until 20 dpp. Moreover, 2 to 3 antral follicles from 20 to 30 dpp were strongly stained for inhibin beta(A) subunit. There was relatively high immunoactivity for inhibin beta(B) subunit in neonatal to 30 dpp mouse ovaries. All three inhibin subunits were stained in theca-interstitial cells from 15 dpp onward. RIA data showed that a temporal increase in circulating FSH occurred around 10 dpp, while the plasma concentrations of LH were sustained at a relatively higher level from 8 to 15 dpp. Inhibin B was detectable in circulation early at 1 dpp (day of birth), and a clear increase in inhibin B occurred around 8 dpp. Circulating inhibin B gradually increased from 20 dpp to 30 dpp, indicating a negative correlation with FSH. Inhibin A levels were only measured on 25 and 30 dpp, and the levels were low. These results suggest that inhibins play an important role in early folliculogenesis in mice. In addition, inhibin B seems to be the main functional isoform from the neonatal to prepubertal stage in the mouse ovary. 相似文献
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Yujin Kim Joong-Sun Kim Myoung-Sub Song Heung-Sik Seo Jong Choon Kim Chun-Sik Bae Seungjoon Kim Taekyun Shin Sung-Ho Kim Changjong Moon 《Journal of veterinary science (Suw?n-si, Korea)》2008,9(4):345-349
Inhibin, which is important for normal gonadal function, acts on the pituitary gonadotropins to suppress follicle-stimulating hormone (FSH) secretion. The level and cellular localization of the inhibin isotypes, α, βA and βB, in the testis of mice were examined during postnatal development in order to determine if inhibin expression is related to testicular maturation. Mouse testes were sampled on postnatal days (PNDs) 1, 3, 6, 18, 48 and 120, and analyzed by Western blotting and immunofluorescence. Western blot analysis showed very low levels of inhibin α, βA and βB expression in the testes at days 1 to 6 after birth. The levels then increased gradually from PND 18 to 48-120, and there were significant peaks at PND 48. Inhibin α, βA and βB were detected in testicular cells during postnatal development using immunohistochemistry. The immunoreactivity of inhibin α was rarely observed in testicular cells during PND 1 to 6, or in the cytoplasmic process of Sertoli cells surrounding the germ cells and interstitial cells during PND 18 to 120. Inhibin βA and βB immunoreactivity was rarely observed in the testis from PND 1 to 6. On the other hand, it was observed in some spermatogonial cells, as well as in the interstitial space between PND 48 and PND 120. We conclude that the expression of inhibin isotypes increases progressively in the testis of mice with increasing postnatal age, suggesting that inhibin is associated with a negative feedback signal for FSH in testicular maturation. 相似文献
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Sakurada Y Sawai M Inoue K Shirota M Shirota K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(7):923-926
In order to understand ovarian toxicity of aryl hydrocarbon receptor (AhR) agonists, in situ gene expression of the AhR was examined during follicle development in immature rats. In situ hybridization on frozen sections of ovaries from 24-day-old Sprague-Dawley rats showed that the AhR mRNA was localized in the granulosa cells and occasionally in the theca cells of the follicles irrespective of the developmental stage. In situ gene quantification on granulosa cell layers collected by laser microdissection further revealed that the granulosa cells expressed less AhR mRNA according to development of belonging follicles, but more β-subunit of inhibin A mRNA, a quality control gene. These results may help to elucidate vulnerable developmental stages of follicles to toxicities of the AhR agonists. 相似文献
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Weng Q Medan MS Ren L Watanabe G Arai KY Taya K 《The Journal of reproduction and development》2005,51(4):521-526
The objective of this study was to investigate the cellular immunolocalization of inhibin alpha and inhibin/activin (betaA and betaB) subunits in the fetal, neonatal and adult testes of Shiba goats. The testes were obtained from a fetus at 90 days, a neonate at 15 days, and two adult Shiba goats (both of 3 years old). The sections of testes were immunostained by the avidin-biotin-peroxidase complex method (ABC) using polyclonal antisera raised against porcine inhibin alpha, inhibin/activin betaA, and inhibin/activin betaB. Inhibin alpha and inhibin/activin (betaA and betaB) subunits were expressed in Leydig cells, but not in the Sertoli cells of the fetus with a weak immunostaining. An increase in the number of positive cells and a more intense immunohistochemical signal for inhibin alpha and inhibin/activin (betaA and betaB) subunits were observed in the Leydig cells of neonatal testes. Moreover, inhibin alpha, betaA, and betaB subunits were expressed in the Sertoli cells and Leydig cells of adult testes, respectively. These results suggest that Shiba goats testes have the ability to synthesize inhibins in the fetus, neonate, and adult, and the cellular localization of inhibin/activin subunits showed age-related changes in fetal, neonatal, and adult testes of Shiba goats. 相似文献
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Peixin YANG Mohamed S. MEDAN Koji Y. ARAI Wanzhu JIN Gen WATANABE Kazuyoshi TAYA 《Animal Science Journal》2005,76(5):427-434
The ontogeny of testicular inhibin/activin in ducks was investigated. Testicular localization of three inhibin/activin subunits (α, βA and βB) was determined in embryonic and newly hatched ducks from 12 days of incubation to 1 day of age, in immature ducks and in adult ducks. In the duck embryonic testis, positive α‐subunit immunostaining was first detected in the Leydig cells and Sertoli cells on day 15 of incubation, whereas βA‐subunit and βB‐subunit immunostaining were found in Sertoli cells and primary germ cells on day 18 of incubation. In 1 month old ducks, intense staining of α‐subunit was present in the seminiferous epithelium consistent with localization in Sertoli cells and primary germ cells, and the immunostaining of the βA‐ and βB‐subunit was also present in Sertoli cells and primary germ cells. Specific immunostaining with inhibin/activin α‐, βA‐ and βB‐subunits antisera occurred in Sertoli cells in the adult duck testes. In conclusion, it was shown that, in the duck testis, the majority of α‐, βA‐ and βB‐subunits are colocalized in Sertoli cells with a certain degree of staining in germ cells and the α‐subunit is present in Leydig cells of embryonic testes before day 18 of incubation. These results indicate that Sertoli cells and possibly germ cells in the embryonic testes of late stage of incubation and newly hatched ducks, immature ducks and mature ducks may produce bioactive inhibin dimers, inhibin A and inhibin B, as a possible regulator of follicle‐stimulating hormone secretion. Free inhibin/activin subunits and their dimers may also play an autocrine/paracrine role in the development of the testis and spermatogenesis. Furthermore, early onset of the α‐subunit in duck testes indicates that it may have an autocrine/paracrine effect on steroid hormones, which is important for sex differentiation. 相似文献
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抑制素是由动物性腺分泌的异二聚体蛋白激素，它通过选择性抑制垂体促性腺激素（主要是FSH）的分泌，进而影响动物的生殖活动。而利用抑制素对动物主动或被动免疫，可增加排卵率，提高繁殖力的独特功能越来越受到人们的重视。本文就近年来有关抑制素免疫对动物生殖功能影响的研究作一综述。 相似文献