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1.
Chun-Ming Lin Benjamin Hause Deanna Gualtieri Nicholas Robinson 《Journal of veterinary diagnostic investigation》2021,33(6):1172
Eight of 9 juvenile raccoons at a rehabilitation center died without obvious prior clinical signs. Gross changes were unremarkable except for mildly distended intestines. Microscopically, crypt loss, distension, necrosis, and regeneration with intranuclear viral inclusions were observed in the small intestine, with marked lymphoid depletion and necrosis in Peyer patches and mesenteric lymph nodes. Immunohistochemistry with a canine parvovirus antibody showed intensive signals of parvoviral antigens in the crypts and lymphoid germinal centers. Metagenomic sequencing allowed assembly of a complete parvoviral genome with >99% identity to canine parvovirus 2a, as well as Salmonella enterica subsp. enterica. Also, S. enterica subsp. enterica serovar Thompson with multiple antimicrobial resistance was isolated from the intestinal contents. Concurrent infection with parvovirus and Salmonella should be included as a differential diagnosis in raccoons with sudden death. 相似文献
2.
Arno Wünschmann Robert Lopez-Astacio Aníbal G. Armin Leslie Reed Colin R. Parrish 《Journal of veterinary diagnostic investigation》2021,33(1):140
A juvenile raccoon was euthanized because of severe neurologic signs. At postmortem examination, no significant gross lesions were present. Histologic evaluation demonstrated nonsuppurative encephalitis in thalamus, brainstem, and hippocampus, cerebellar Purkinje cell loss, as well as poliomyelitis and demyelination of the spinal cord. Parvovirus antigen–specific immunohistochemistry revealed immunopositive neurons in the brainstem, cerebral cortex, and hippocampus. A few Purkinje cells were also immunopositive. DNA extracted from formalin-fixed, paraffin-embedded brain tissue (thalamus, hippocampus, cerebral cortex) yielded a positive signal using PCR targeting both feline and canine parvovirus. Sequencing analyses from a fragment of the NS1 gene and a portion of the VP2 gene confirmed the presence of DNA of a recent canine parvovirus variant (CPV-2a–like virus) in the cerebellum. Our case provides evidence that a recent canine parvovirus (CPV) strain (Carnivore protoparvovirus 1) can infect cerebral and diencephalic neurons and cause encephalitis in an otherwise healthy raccoon. Parvovirus-induced encephalitis is a differential diagnosis of rabies and canine distemper in raccoons with neurologic signs. 相似文献
3.
Junko SUZUKI Yohei NISHIO Yuki KAMEO Yutaka TERADA Ryusei KUWATA Hiroshi SHIMODA Kazuo SUZUKI Ken MAEDA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(11):1457-1463
In 2007–2008, a canine distemper virus (CDV) epidemic occurred among wild animals in
Wakayama Prefecture, Japan, and many mammals, including the wild boar and deer, were
infected. In this study, CDV prevalence among wild animals was surveyed before and after
the epidemic. At first, an enzyme-linked immunosorbent assay (ELISA) with horseradish
peroxidase-conjugated protein A/G was established to detect CDV antibodies in many
mammalian species. This established ELISA was available for testing dogs, raccoons and
raccoon dogs as well as virus-neutralization test. Next, a serological survey of wild
mammalians was conducted, and it was indicated that many wild mammalians, particularly
raccoons, were infected with CDV during the epidemic, but few were infected before and
after the epidemic. On the other hand, many raccoon dogs died during the epidemic, but CDV
remained prevalent in the remaining population, and a small epidemic occurred in raccoon
dogs in 2012–2013. These results indicated that the epidemic of 2007–2008 may have been
intensified by transmission to raccoons. 相似文献
4.
A N Hamir N Raju C Hable C E Rupprecht 《Journal of veterinary diagnostic investigation》1992,4(2):159-163
A 6-year (1985-1990) retrospective survey of raccoons with canine distemper infection and an outbreak of the disease in 1988 on South Island, South Carolina, are described. During this epizootic, 3 male raccoons with the clinical disease had gross testicular lesions that, on histopathologic examination, revealed severe diffuse degeneration and mineralization of seminiferous tubules. The testicular pathology of canine distemper in raccoons has not been previously reported. 相似文献
5.
AbstractCASE HISTORY: One 4.5-month-old male Border Collie cross presented with aggression and seizures in October 2006. A 16-month-old, female, spayed Border Collie cross presented with hypersalivation and a dropped jaw and rapidly became stuporous in September 2007. The dogs were littermates and developed acute neurological signs 5 and 27 days, respectively, after vaccination with different modified live vaccines containing canine distemper virus.HISTOPATHOLOGICAL FINDINGS: Sections of brain in both dogs showed evidence of encephalitis mainly centred on the grey matter of brainstem nuclei, where there was extensive and intense parenchymal and perivascular infiltration of histiocytes and lymphocytes. Intra-nuclear and intra-cytoplasmic inclusions typical of distemper were plentiful and there was abundant labelling for canine distemper virus using immunohistochemistry.DIAGNOSIS: Post-vaccinal canine distemper.CLINCIAL RELEVANCE: Post-vaccinal canine distemper has mainly been attributed to virulent vaccine virus, but it may also occur in dogs whose immunologic nature makes them susceptible to disease induced by a modified-live vaccine virus that is safe and protective for most dogs. 相似文献
6.
Tumour necrosis factor‐alpha‐induced protein 8 (TNFAIP8) expression associated with cell survival and death in cancer cell lines infected with canine distemper virus 
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下载免费PDF全文 J. A. Garcia H. L. Ferreira F. V. Vieira R. Gameiro A. L. Andrade F. R. Eugênio E. F. Flores T. C. Cardoso 《Veterinary and comparative oncology》2017,15(2):336-344
Oncolytic virotherapy is a novel strategy for treatment of cancer in humans and companion animals as well. Canine distemper virus (CDV), a paramyxovirus, has proven to be oncolytic through induction of apoptosis in canine‐derived tumour cells, yet the mechanism behind this inhibitory action is poorly understood. In this study, three human mammary tumour cell lines and one canine‐derived adenofibrosarcoma cell line were tested regarding to their susceptibility to CDV infection, cell proliferation, apoptosis, mitochondrial membrane potential and expression of tumour necrosis factor‐alpha‐induced protein 8 (TNFAIP8). CDV replication‐induced cytopathic effect, decrease of cell proliferation rates, and >45% of infected cells were considered death and/or under late apoptosis/necrosis. TNFAIP8 and CDVM gene expression were positively correlated in all cell lines. In addition, mitochondrial membrane depolarization was associated with increase in virus titres (p < 0.005). Thus, these results strongly suggest that both human and canine mammary tumour cells are potential candidates for studies concerning CDV‐induced cancer therapy. 相似文献
7.
Jae-Ik Han Dong-Woo Chang Ki-Jeong Na 《Journal of veterinary science (Suw?n-si, Korea)》2015,16(3):341-347
Meningoencephalitis (ME) is a common inflammatory disorder of the central nervous system in dogs. Clinically, ME has both infectious and non-infectious causes. In the present study, a multiplex quantitative real-time polymerase chain reaction (mqPCR) panel was optimized for the detection of eight canine neurologic pathogens (Blastomyces dermatitidis, Cryptococcus spp., Neospora caninum, Borrelia burgdorferi, Bartonella spp., Toxoplasma gondii, Ehrlichia canis, and canine distemper virus [CDV]). The mqPCR panel was subsequently applied to 53 cerebrospinal fluid (CSF) samples collected from dogs with ME. The analytic sensitivity (i.e., limit of detection, expressed as molecules per 1 µL of recombinant vector) was 3.8 for CDV, 3.7 for Ehrlichia canis, 3.7 for Bartonella spp., 3.8 for Borrelia burgdorferi, 3.7 for Blastomyces dermatitidis, 3.7 for Cryptococcus spp., 38 for Neospora caninum, and 3.7 for Toxoplasma gondii. Among the tested CSF samples, seven (15%) were positive for the following pathogens in decreasing order of frequency: Cryptococcus spp. (3/7), Blastomyces dermatitidis (2/7), and Borrelia burgdorferi (2/7). In summary, use of an mqPCR panel with high analytic sensitivity as an initial screen for infectious agents in dogs with ME could facilitate the selection of early treatment strategies and improve outcomes. 相似文献
8.
临床中犬瘟热多分为急性和亚急性,3月龄犬多发本病,急性病例和亚急性病例的临床症状和病毒复制的脏器与慢性病例有很大区别。临床上急性病例的病变主要集中在肺脏和淋巴组织,组织病理学可观察到淋巴结炎和多核巨细胞性肺炎。根据免疫组织化学染色结果发现,在犬瘟热急性病例的淋巴结皮质中犬瘟热抗原呈强阳性,且密度很高。而病犬虽然表现出严重的肺炎症状,但在犬的肺脏中犬瘟热病毒抗原阳性反应弱,仅在支气管上皮和少量的巨噬细胞胞浆中观察到。犬瘟热病毒首先在犬的淋巴组织中进行复制、增殖,破坏被膜下淋巴窦和淋巴小节中的T淋巴细胞和B淋巴细胞,影响淋巴组织输出到外周血液循环中的淋巴细胞数量。本研究结果表明,犬瘟热虽然临床上以肺脏病变最严重,但在急性和亚急性病例,犬瘟热病毒主要在淋巴组织的T淋巴细胞、B淋巴细胞和巨噬细胞中增殖,故应以淋巴组织作为犬瘟热病毒的主要分离脏器。 相似文献
9.
犬细小病毒病的流行现状调查 总被引：3，自引：0，他引：3
对犬细小病毒病流行现状进行调查,为本病的预防、诊断和治疗提供依据。 用犬细小病毒抗原检测试剂盒对2008年门诊的具有腹泻、呕吐、粪便带血等体征的189只患犬作病原检测,对犬细小病毒阳性病例兼有发热、咳嗽体征的16例病犬同时作犬瘟热病毒抗原检测。结果犬细小病毒阳性病例共137只,发病年龄从1个月～15岁各年龄段均有分布,以6月龄以下幼龄动物发病较多,占65.7%。流行季节差异不大,以春季病例略多,占27.0%。犬细小病毒与犬瘟热病毒混合感染的有12例,占8.8%。对在疫苗接种免疫保护期内的4只患犬进行检测,3只犬细小病毒阳性。结论：①免疫力尚未建立是幼犬发病的主要原因;②不按规定的免疫程序对动物进行加强免疫是成年犬发病的主要原因;③在部分发病幼犬中存在犬细小病毒与犬瘟热病毒的混合感染;④老龄动物对免疫的应答能力下降可导致免疫失败。 相似文献
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小熊猫犬瘟热病及病原研究 总被引：2，自引：0，他引：2
1999年2月和1999年7月，重庆动物园和雅安碧峰峡生态动物园喂养的小熊猫（red panda)分别大面积爆发和流行犬瘟热病，重庆动物园小熊猫死亡11只，死亡率达100％，雅安碧峰峡生态动物园小熊猫死亡4只，死亡率为25％。两地发病小熊猫所表现的临床症状和病理解剖变化不完全一致，但均有血便、呼吸急促、不食，精神差，肺出血充血等类似症状。从雅安碧峰峡生态动物园患病小熊猫分离出的犬瘟热毒株（cdv2株）对VERO细胞适应性比从重庆动物园分离的（cdv1株）强，其TCID50达10^-6/0.2mL，两地分离的毒株均能同抗犬瘟热阳性高免血清反应。此外，用抗犬瘟热高免血清治疗，成功地挽救了12只病重的小熊猫。 相似文献
11.
犬瘟热是由犬瘟热病毒引起的犬科烈性传染病,给患病动物带来重大危害。本研究基于犬瘟热病毒的NP蛋白基因设计特异性引物,利用RT-PCR获得287 bp目的片段,经TA克隆并测序。结果表明,该片段与NCBI上公布的犬瘟热病毒NP序列(登录号:EU716322)同源性达到98%。利用该特异性的RT-PCR方法,检测杭州市及周边部分地区犬瘟热的流行情况,结果表明,整个杭州及周边地区犬瘟热病毒总阳性检出率为6.7%,杭州城区阳性个体数最多,阳性率最高达到18.8%,其他地方(临安、台州、舟山)阳性率较低。该研究为犬瘟热的综合防制提供基础数据。 相似文献
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Shotaro KUBO Morihiro TATENO Yasuaki ICHIKAWA Yasuyuki ENDO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(10):1275-1279
Tick-borne diseases are often encountered in canine clinical practice. In the present
study, a molecular epidemiological survey of dogs in Japan was conducted to understand the
prevalence and geographical distribution of Babesia spp.,
Hepatozoon spp., Ehrlichia spp. and
Anaplasma spp. Pathogen-derived DNA in blood samples obtained from 722
dogs with a history of exposure to ticks and/or fleas was examined by PCR. The prevalence
of Babesia gibsoni, Babesia odocoilei-like species,
Hepatozoon canis and Ehrlichia
spp./Anaplasma spp. was 2.4% (16/722), 0.1% (1/722), 2.5% (18/722) and
1.5% (11/722), respectively. While B. gibsoni and
Ehrlichia spp./Anaplasma spp. were detected in the
western part of Japan, H. canis was detected in Tohoku area in addition
to western and central parts of Japan. 相似文献
14.
水貂犬瘟热流行病学调查 总被引：1，自引：0，他引：1
为了调查山东省水貂犬瘟热的流行病学情况,对2010年8月－2012年8月期间由诸城、文登等地养貂场送诊的996份水貂病例进行了临床诊断和病原学检查。结果显示,共检出犬瘟热病毒阳性病料223份,阳性率为22.39%。其中,犬瘟热病毒与其他病原混合感染136例,占阳性病例的60.99%;阳性样本中未免疫病例116个;诸城检出阳性病例数最多,为147例;3月龄水貂发病率最高,占阳性病例的43.0%;水貂感染犬瘟热病毒主要症状为眼、鼻黏液性分泌物增多、脚垫增厚、呼吸困难;主要病理变化为肠、脑部出血。结果表明,犬瘟热依然是危害水貂养殖的重要疫病,且犬瘟热病毒易与其他病原混合感染;免疫接种能有效预防水貂犬瘟热,且宜在3月龄以前进行。 相似文献
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犬瘟热病毒细胞受体研究进展 总被引：1，自引：1，他引：0
犬瘟热病毒是一种引起犬类、毛皮动物、野生动物和部分海洋哺乳动物发生多系统感染的病毒性传染病。犬瘟热的宿主范围和组织嗜性主要由其感染动物的细胞表面受体决定。目前,已经确认的犬瘟热受体有信号淋巴细胞激活因子。为了更好地对犬瘟热病毒的受体进行研究,就当前犬瘟热病毒的受体研究进行综述。 相似文献
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小熊猫源犬瘟热病毒全基因序列的克隆及序列分析 总被引：2，自引：1，他引：1
本研究对军事兽医研究所病毒二室分离驯化后的小熊猫源犬瘟热病毒(canine distemper virus,CDV)基因组进行全序列测定,并对其基因组特征及H基因遗传稳定性进行比较分析。根据GenBank公布的犬瘟热病毒全长基因组序列设计合成17对特异性引物,以小熊猫源犬瘟热病毒总RNA为模板,RT-PCR进行分段扩增,并克隆到pEASY-Blunt Simple载体中,经测序、拼接获得全长cDNA序列。结果显示,小熊猫源犬瘟热病毒全基因序列与GenBank登录号分别为AF014953、AY445077、AY542312、AY466011、AY386316、AF164967、EU716337、EU726268、AY443350、AB474397、GU138403和AY649446的12个不同毒株全基因序列的同源性分别为86.6%、92.4%、92.5%、92.5%、94.3%、96.3%、95.9%、87.1%、94.5%、92.3%、87.4%和94.6%,与标准强毒株A75/17株(AF164967)的亲缘关系最近,全基因同源性达96.3%,但与疫苗株Onderstepoort(AF014953)亲缘关系相对较远,同源性为86.6%。小熊猫源犬瘟热病毒H基因与其他不同地区具有代表性的30株CDV进化树分析显示,小熊猫源犬瘟热病毒属于Asia Ⅰ型,H蛋白中309－311位氨基酸残基所形成的潜在糖基化位点,为疫苗株没有而野毒株所共有的,并且可能与病毒的免疫原性有关。因此,致弱的小熊猫源CDV在预防免疫的针对性上可能强于已有的疫苗株。 相似文献
19.
Canine Distemper in Wild Raccoons (Procyon lotor) at the Metropolitan Toronto Zoo 总被引：1，自引：0，他引：1 下载免费PDF全文
下载免费PDF全文 Cranfield MR Barker IK Mehren KG Rapley WA 《The Canadian veterinary journal. La revue veterinaire canadienne》1984,25(2):63-66
An outbreak of canine distemper, involving at least 23 wild raccoons, occurred at the Metropolitan Toronto Zoo between May and August 1981. Wild red foxes were not found with distemper and the disease did not occur in the captive collection. The main clinical signs were lethargy, bilateral purulent ocular discharge, dehydration and emaciation. The main pathological findings were generalized lymphadenopathy, splenomegaly, and interstitial pneumonia. Heavy infestations with intestinal nematodes (Baylisascaris procyonis), and unidentified cestodes were found. Cryptosporidia were found on the epithelium of the duodenum in 42% of the animals examined. Eosinophilic intracytoplasmic and intranuclear inclusions were numerous in the epithelium of the urinary bladder, renal pelvis, stomach and lung. This local outbreak was part of a major eruption of the disease in raccoons in southern Ontario which began in early 1981 and persisted until the submission of this paper in mid 1983. 相似文献
20.
In recent years, canine distemper is a serious damage to health development of dog and fur animal breeding industry.Fast and accurate diagnostic technique is particularly important for canine distemper prevention.At the present stage, canine distemper diagnostic techniques mainly include virus isolation, culture and microscopy observation, enzyme-linked immunosorbent assay(ELISA), immunohistochemistry, immune colloidal gold technique, nucleic acid hybridization technique, nested RT-PCR, Real-time fluorescence quantitative RT-PCR, double PCR, gene chip technology, liquid phase chip technology, loop-mediated isothermal amplification technology, and so on.The key of isolation, culture and microscopy observation of canine distemper virus is to obtain a susceptible cell lines that can produce typical CPE.We review research progress on diagnostic techniques of canine distemper, so as to provide simple and highly effective detecting technique for preventing and controlling epidemic and outbreak of canine distemper. 相似文献