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1.
Vernonia galamensis is a wild plant from the family Asteraceae which is endemic to East Africa and has the potential to become a new oil crop for industrial uses. Its seed oil is rich in vernolic acid, a fatty acid of high interest for oleochemical applications. However, a breeding program for Vernonia galamensis cultivars with high seed and oil yields requires knowledge about the genetic variability of traits that influence seed and oil production. This study was undertaken to examine phenotypic and genotypic variability, broad-sense heritability, genetic advance under selection and interrelationships of agronomic and seed quality traits. A total of 122 Vernonia accessions, 115 collected from different regions of Ethiopia and seven introduced, were grown at two locations in Ethiopia (Alemaya and Babile), in 2001/2002 and were analyzed for 20 traits including phenology, yield, yield components, and seed quality with special emphasis on fatty acid composition. The collections exhibited significant variation for all traits except for days to emergence. Genotypes and locations interacted significantly (P 0.01) for all traits. Broad-sense heritability estimates ranged from 11% (for days to emergence) up to 79% (for days to maturity). Expected genetic advance was between 1.3% (for days to emergence) and 44.8% (for seed oil yield). Genetic correlation analysis revealed that seed yield per plant is highly and positively correlated with seed weight and head number; highly significant and negative correlations (r = –0.59, –0.82, –0.85, and –0.89) were found between vernolic acid and palmitic, stearic, oleic, and linoleic acid, respectively. Highly significant positive correlations (r = 0.55, 0.44, and 0.36) were observed between vernolic acid and oil content, meal protein content and seed oil yield, respectively. Path-coefficient analysis indicated seed weight and secondary head number to be the most important components of seed yield per plant. Vernolic acid, oleic acid and linoleic acid had positive direct effects and stearic acid had a negative direct effect on oil content. The direct positive effect of oleic acid on oil content was, however, compensated by the negative indirect effects of stearic and vernolic acid resulting in a negative correlation (r = –0.60) between oleic acid and oil content. These observations will support the selection of accessions with high seed and oil yield, high meal protein contents, and high vernolic acid content. 相似文献
2.
Five cycles of phenotypic recurrent selection for increased essential oil content were carried out in East Indian lemongrass, Cymbopogon flexuosus. In each cycle, the top 5% of plants for essential oil content were selected and their ramets were planted in isolated polycross blocks to produce the seed of the next cycle. Response to selection for essential oil content and its effect on three unselected traits, leaf yield, dry matter content in leaves and citral content in the essential oil, were determined by evaluating C0‐C5 populations in a replicated trial. Genetic variation, heritability estimates and intertrait correlations for essential oil content, leaf yield, leaf width, tiller number and citral content in the essential oil in C4 were determined, by evaluating 40 clones and their half‐sib progenies produced from 40 randomly selected C4 plants, in a replicated experiment. These were compared with those determined in C0 earlier. The mean essential oil content increased from 0.66% in C0 to 1.67% in C5 (i.e. by about 31% per cycle over C0). Selection for essential oil content did not affect leaf yield and its effect on dry matter content in leaves was marginal. The first three cycles of selection for essential oil content did not affect citral content in the oil but two further cycles significantly decreased citral content. Heritabilities and intertrait correlations between all traits studied, except citral content and essential oil content, were similar to those found in C0. 相似文献
3.
Nagawara Seshagirirao Ravindra Srinivas Iyer Ramesh Mahesh Kumar Gupta Tripta Jhang Ashutosh Kumar Shukla Mahendra Pandurang Darokar Raghavendra Narayanrao Kulkarni 《Journal of Crop Science and Biotechnology》2012,15(1):33-39
Patchouli (Pogostemon patchouli) is an important, exclusively vegetatively propagated aromatic plant, whose essential oil is widely used in perfumery and
cosmetic products. Forty SC1 generation (first generation following in vitro phase) somaclones selected randomly from about 400 somaclones developed from the variety Johore, were multiplied through
stem cuttings and evaluated in SC2 and SC3 generations to study the extent of somaclonal variation generated for plant height, herb yield, essential oil content, essential
oil yield, and seven constituents of the essential oil. Significant or highly significant somaclonal variation was observed
for plant height, herb yield, essential oil content, essential oil yield, and contents of patchouli alcohol, α-guaiene, α,δ-patchoulene,
and α-bulnesene in the essential oil. The number of somaclones significantly superior to the parental variety for plant height,
herb yield, essential oil content, and patchouli alcohol content in the essential oil ranged from 8–16 and the maximum superiority
over the parental variety for these traits ranged from 21–79%. Broad-sense heritability estimates of plant height, herb yield,
and essential oil content were 0.60–0.70 while those of essential oil yield and patchouli alcohol content were 0.44 and 0.47,
respectively. Heritability estimates of other studied essential oil constituents were generally low (0.12–0.38). A high positive
correlation was observed between essential oil yield and herb yield suggesting that selection for herb yield would be effective
in improving essential oil yield. Patchouli alcohol content in the essential oil was negatively correlated with all the studied
traits. Somaclonal variation, heritabilities of traits, and inter-trait correlations are reported for the first time in patchouli. 相似文献
4.
L. George V. Abraham D. R. Suryavanshi A. T. Sipahimalani V. T. Srinivasan 《Plant Breeding》1987,98(1):72-74
Homozygous diploids were obtained through anther culture in Indian mustard, Brassier juncea, cultivar TM-4, Seeds could be harvested from 97 such plants in the A; generation. The androgenetic lines were further selected and the yield and yield components were compared in the A4 and A5 generations, Androgenetic lines showed variations for most of the characters studied including seed oil content and fatty acid composition. However, it was possible to recover seven lines out of 25 which were equal in seed yield and three equal in oil content compared TO the anther donor parent TM-4. 相似文献
5.
Summary Anthers of the diploid (2n=77) and the colchi-tetraploid (2n=154) Pelargonium roseum were cultured in vitro. In both ploidy level anthers containing uninucleate or binucleate microspores were incubated on a modified White's medium. Calli formed were then subcultured on Murashige and Skoog's medium for organoid differentiation. Plants developed from organoids were transferred to filter paper bridges and after that transplanted into pots. Plants derived from anthers of the tetraploid had diploid chromosome number. Wide variation of their essential oil components suggested their genetic heterogeneity. Further, high correlations between different seasons in the rate of essential oil components showed that the wide variation was due to genetic differences. Therefore, these plants probably originated from pollen grains. On the other hand, plants derived from anthers of the diploid had diploid chromosome number. Small variation and low correlations between different seasons in essential oil components indicated their genetic homogeneity. Their origin was ascribable to the somatic tissues of the mother plant. It is concluded that in plant species in which usual sexual reproduction is difficult, anther culture of chromosome-doubled plants will give a useful method for obtaining genetic variation. 相似文献
6.
Evaluation of a lucerne germplasm collection in relation to traits conferring grazing tolerance 总被引：2，自引：0，他引：2
Summary A collection of 90 accessions of different germplasm groups from the Medicago sativa complex was evaluated at Lodi, Italy, for dry-matter yield, time to anthesis, height of regrowth, plant diameter, stem density, vegetative recovery after winter, and presence of deep-set crown and ability to spread with creeping roots or rhizomes, these latter characters possibly contributing to grazing tolerance. The germplasm groups proved rather distinct for these traits, but all included types potentially suitable for grazing. Interesting types combining deep-set crown and high yield occurred mainly in subsp. x varia and materials originated from artificial crosses involving at various extent the subspecies falcata, sativa and x varia. Though belonging to subsp. sativa, mielga wild populations from Spain had aerial traits similar to those of the long-dormant, low-yielding subsp. falcata. Deep-crowned plants had greater plant diameter than shallow-crowned plants, and could be categorised into four distinct top-growth models according to their habit and vigour. One of these models combined spreading ability with high yield. The about 150 plants selected for their combination of deep crown, wide diameter, and good yield were characterised by a branch-rooted system. There was general correspondence between top-growth and root morphology of each model. Of the four deep-crowned models, three had rhizomes, with wide range of underground development and morphology, while the fourth had creeping roots. The latter model was the most rare among the examined accessions, never appearing in either cultivated or wild subsp. sativa. The implications of the results on the development of grazing-tolerant lucerne varieties are discussed. 相似文献
7.
Elaeis oleifera or ‘caiaué’, a close relative of oil palm (E. guineensis), has some agronomic traits of great interest for the oil palm genetic breeding such as slow growth, oil quality (mostly
unsaturated) and disease resistance. An analysis of a Brazilian oil palm germplasm collection was carried out using RAPD (Random
Amplified Polymorphic DNA) markers with the objective of understanding the genetic variation of ‘caiaué’ accessions collected
in the Amazon Forest in the last two decades. A sample of 175 accessions obtained along the Amazon River Basin was analyzed
and compared to 17 accessions of oil palm from Africa. Ninety-six RAPD markers were used in the analysis, of which fourteen
were shown to be specific to oil palm, while twelve were specific to ‘caiaué’. Results showed that the Brazilian ‘caiaué’
accessions studied have moderate levels of genetic diversity as compared to oil palm accessions. The data allowed the establishment
of similarity groups for ‘caiaué’ accessions, which is useful for selecting parental plants for population breeding. Cluster
analysis showed that, in general, genetic similarities are not correlated to geographical distances, but consistent with geographical
dispersal along the Amazon River network. AMOVA showed that most of the genetic variation is found within populations, as
expected for anallogamous and long-lived perennial species. The study provides important information to define strategies
for future collection expeditions, for germplasm conservation and for the use of E. oleifera in breeding programs.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
8.
Somaclonal variation for plant height, plant spread, leaf shape, leaf size, leaf form, herb yield, essential oil content and 10 important constituents of the essential oil was studied in an Indian cultivar, ‘Bourbon’ of rose‐scented geranium (Pelargonium graveolens). Significantly larger variance was observed among in vitro‐regenerated plants of the SC1 generation (first generation following an in vitro phase) than among parental plants raised from stem cuttings for herb yield, plant height, leaf size, essential oil content, and for the contents of cis‐rose oxide, trans‐rose oxide, isomenthone and 10‐epi‐γ‐eudesmol in the essential oil. Plants of the SC1 generation showing values beyond the higher or lower limit (depending on the trait) of the range observed among parental plants for different traits were developed into clones by their multiplication through stem cuttings, and evaluated again in the SC2 generation (second generation following the in vitro phase). Somaclones selected for plant spread, and for the contents of linalool and trans‐rose oxide in their essential oils did not breed true in the SC2 generation. However, for the remaining traits studied, 13–100% of the selected clones retained their selected traits in the SC2 generation, suggesting that the changes in these somaclones were due to genetic causes. 相似文献
9.
The pentaploid hybrid of Lolium multiflorum and Festuca arundinacea (2n = 5x = 35) combines the high growth rate of L. multiforum with the drought resistance and freezing-tolerance of F. arundinacea. Unfortunately, it also displays the deleterious traits associated with Festuca, namely those associated with high leaf fibre content giving rise to poor palatability and digestibility. To access different
combinations of these characters, anther cultures were initiated and regenerated into single embryo derived plants. The anther
culture method was very productive since out of a total of 2349 androgenic plants derived from the same parent plant, 57%
were green plantlets, although only 507 (22%) subsequently established into plants following transfer to soil. Chromosome
counts of randomly selected lines showed that plants with euploid chromosome numbers (14, 21, and 28) would appear to have
selective advantage during regeneration. There was wide variation between mature androgenic plants grown under field conditions
in plant height, leaf length, leaf width, tiller number and herbage dry matter. The variation between genotypes in response
to drought stress was assessed by placing replicate clones under rain-out shelters or under irrigated control conditions in
the field. Herbage dry matter under drought was higher in a number of androgenic lines than either parents, but not higher
than the pentaploid hybrid. Androgenesis was shown to be a highly effective procedure to expose diverse phenotypic variation
all derived from the same Lolium × Festuca hybrid genotype.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
10.
Summary Ten anther-derived monoploids, twelve doubled monoploids and the heterozygous anther donor clone of Solanum phureja were compared for six vegetative, eight reproductive and three yield characters in the greenhouse. Significant high correlations were observed between the monoploids and their doubled monoploids for 14 of 17 characters. Therefore, doubled monoploids presumably express the same alleles as monoploids and the phenotypic expression of a monoploid can be used to predict accurately the phenotype of the derived doubled monoploid. Doubled monoploids flowered earlier and had significantly higher values than the monoploids for 15 of 17 characters, indicating a positive effect of increasing gene dosage from monoploid to diploid. Morphological traits can be used to distinguish plants of the two ploidy levels. Significant differences among clones were observed for all parameters within each ploidy level. The anther donor was not significantly different from the mean of doubled monoploids for 10 of 17 characters. For each of 14 characters, some doubled monoploids exceeded the anther donor. Therefore, doubled monoploids which do not express homozygote depression can be obtained. 相似文献
11.
R. N. Kulkarni 《Euphytica》1991,52(2):99-102
Summary Three cycles of honeycomb selection for herb yield, applied on widely spaced individual plants of davana (Artemisia pallens Wall.), resulted in an increase in yield of 12.4% per cycle when the resulting populations were evaluated at commercial plant density. The selection had no adverse effect on essential oil content, davanone content in oil and plant height. 相似文献
12.
The ratio of monounsaturated oleic fatty acid to polyunsaturated linoleic fatty acid (O/L) and the associated agronomic traits
were profiled for local peanut (Arachis hypogaea L.) cultivars and accessions in Botswana. The research was tested on 16 entries planted in four localities across the country.
The average total oil yield of the genotypes studied was about 42.1% and there was no significant difference in total oil
yield (P < 0.05) across the four research sites and among all entries (accessions). The average mean O/L ratio for all entries was
about 1.47. The highest was that of the locally improved variety, Peolwane (2.58), which was significantly different (P < 0.05) from all other accessions, and the lowest is that of the local accession GO 63 (1.23). This study also profiled the
agronomic yield traits to identify genotypes that have both high oil quality and high yielding potential. No correlation was
detected between pod yield and O/L ratio among the tested genotypes. The station with little rainfall but adequate repartition
showed a very high O/L ratio for some genotypes, and future work is recommended to test this interesting observation on the
correlation between moisture stress and oil quality. 相似文献
13.
Helianthus argophyllus is a wild species known as “silver sunflower”, which possesses several traits, including morphological traits that increase drought tolerance. Therefore, introgressions between chosen cultivated lines and two H. argophyllus accessions were made, and segregating generations were established. Important agronomic traits including single heading, high pollen fertility, silver canopy (indicating more cuticular wax), and content of cuticular waxes and oil were selected over six segregating generations. The resulting F6 lines showed introgression of water saving traits, as they had lower excise leaf water loss, with comparable yield to standard checks. However, these F6 lines were late maturing and showed poor flowering synchronization between the cultivated and introgressed lines. Introgressed line “D‐22” was particularly promising as a breeding line, with superior agronomic and drought resistance traits. This line had the potential to be used as an inbred parental line for introgression of drought resistance traits into elite sunflower germplasm. Combining ability analysis of the introgressed lines further showed their potential for heterosis breeding or to be used as parental lines in breeding programme. 相似文献
14.
Stability of foliage yield and its quality components has not been investigated in vegetable chenopods due to their underutilized
status. The objectives of the present study were to assess genotype-environment interactions (GEI), determine stability of
yield and quality components and to compare different parametric and non-parametric stability parameters. The present study
reports for the first time the effect of genotype and environment on foliage yield and three leaf quality traits viz. carotenoid,
ascorbic acid and protein content in different species of Chenopodium. Twenty accessions comprising four species were tested in a randomized block design with three replications across four environments.
For all the four traits the largest sum of squares was accounted for by the genotypes, followed by GEI and environments. Highest
foliage yield of 16.02 q/ha was obtained from C. album PRC 9804, while the lowest was from C. album CHEN 63/80 (4.01 q/ha). Many indigenous accessions of C. album (PRC 9801, IC 107299, ‘Chandigarh’, ‘local’ and ‘NEFA’) were unstable both for foliage yield and most of the quality traits.
Most of the exotic accessions of C. giganteum were both stable and high yielding, thus, reflecting the potential of these accessions for future breeding programs/variety
release. 相似文献
15.
Summary The increasing popularity of organic agriculture and health food products has led to a renewed interest in hulled wheat species such as emmer (Triticum turgidum ssp. dicoccumSchubler). Knowledge on agronomic and quality traits is required for effective and efficient use of germplasm collections in breeding programs. The objective of this study was to estimate agronomic and grain quality traits of emmer wheat cultivated in Italy. A total of 20 emmer accessions consisting of landraces, breeding lines or cultivars selected from landraces and modern cultivars were examined under low input conditions. The study was conducted for three successive years (2002–2004) at one location of Southern Italy (Foggia). The entries were characterized for agronomic and qualitative traits [grain yield (t ha−1), thousand grain weight (g), test weight (kg hl−1), grain protein content (%), HMWG composition, dry gluten content (%), gluten index and yellow index, alveograph indices and Total Organic Matter (TOM) on cooked pasta]. The results showed a large genetic variability for most of the traits measured and, even if most of the accessions showed inferior bread- and pasta-making performance, modern cultivars exhibited improved quality traits with some potential to perform healthy and tasty food. 相似文献
16.
Akshay K. Pradhan Yaspal S. Sodhi Arundhati Mukhopadhyay Deepak Pental 《Euphytica》1993,69(3):219-229
Summary Divergence of 25 accessions of Brassica juncea of Indian, CIS (Commonwealth of Independent States, former USSR) and synthetic origin was studied by D2 analysis. On the basis of divergence, ten accessions were selected and crossed in a diallel fashion without reciprocals to study the combining ability and heterosis. None of the accessions was found to be a good general combiner for all the nine quantitative characters that were studied. Significant heterosis over better parent for single plant yield was recorded in CIS x Indian and synthetic x CIS crosses (5 each) followed by Indian x synthetic types (3). The analysis of component characters showed that the mean performance of the majority of hybrids was intermediate for five out of six yield attributing traits, thus exhibiting dominance or partial dominance effect. To estimate the contribution of such yield attributing traits towards heterosis for yield, a comparison was made among three parameters viz. heterosis over mid parent (MP), better parent (BP) and better yielding parent (BYP) of the concerned hybrid. It was observed that estimation of heterosis from BYP was a more accurate method to determine the contribution of component characters towards yield heterosis than the analysis based on MP and BP. From the component character analysis, it was concluded that characters like number of primary and secondary branches, number of siliqua per plant and siliqua density contributed significantly towards heterosis in yield. Plot level yield trials of two selected hybrids (Skorospieka II x RH30 and Donskaja IV x Varuna) over two growing seasons revealed 29.4 to 91.8% heterosis over BYP. 相似文献
17.
In the present study diversity in a collection of the Garden Beet Group,consisting of 40 accessions from the BAZ Gene Bank
and Polish cultivars, was evaluated in a two-year field trial. Thirteen morphological and yield traits were evaluated as well
as content of betanin, vulgaxanthine, sugars, and nitrates in root tissue. The study revealed that accessions characterised
by circular root shape and dark red skin, flesh and ring colour were the most common. It was also possible to identify several
highly distinct accessions. The results allowed to assess genetic diversity and to make suggestions on rearranging of the
Garden Beet Group core collection, which was previously set up a priori.Accessions showing a high nutritional value, potentially useful for quality breeding, were indicated.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
18.
19.
The present investigation discusses the scope for transferring of resistance to leaf spot disease incited by Alternaria helianthi from two hexaploid wild species (H. tuberosus and H. resinosus) to diploid cultivated sunflower. Interspecific hybrids produced between sunflower and these two hexaploid species were partially fertile with tetraploid chromosome status. Backcrosses of these interspecific hybrids with cultivated sunflower resulted in the formation of sterile triploid plants. To overcome the problem of sterility and facilitate backcrosses with cultivated sunflower, anther culture of the tetraploid interspecific hybrids was carried out to bring down their chromosome number to diploid status. Anthers from both interspecific hybrids were cultured on basal Murashige and Skoog media supplemented with varying concentrations of organics and the growth regulators benzyladenine and naphthaleneacetic acid. Anthers of interspecific hybrids involving H. resinosus responded well and regenerated through an embryogenic route at a frequency of 98.7%. But in interspecific hybrids with H. tuberosus, anthers formed callus and subsequently regenerated shoots through an organogenic pathway. DNA ploidy analysis of anther culture plants of interspecific hybrids derived from H. tuberosus crosses was carried out to identify plants with desired diploid status. In vitro screening of parents, interspecific hybrids and anther culture plantlets against A. helianthi showed resistance in 68.5% of the anther culture plants of interspecific hybrids from H. tuberosus and in 24.3% of the plants derived from interspecific hybrids involving H. resinosus. 相似文献
20.
A procedure has been established for high frequency, long-term plant regeneration from leaf sheath-derived callus cultures a- Citronella Java, variety ‘Jorhat’. A total of 600 plants were successfully transferred to the glass house, and subsequently 500 of these were shifted to the field in TWO batches of 250 plants each. From amongst 230 plants of the firs; hatch, on which data were recorded, extensive somaclonal variations were observed for seven agronomic traits, viz., herbage yield, tiller number, diameter of the bush, length and area of the longest leaf, fresh and’ dry weight ratio and oil content. In addition, variations were also recorded for six major constituents of the essential oil pool, i.e., citronellal, citronellol, geraniol, citronellyl acetate, geranyl acetate and elemol. Correlation analysis between agronomic traits revealed a significant negative correlation between oil content and herbage yield. Haw-ever, plants with higher herbage yield (> 3 fold) and oil content (> 1.5–2.0 fold) than the parent variety could be selected from amongst the variants. Furthermore, plants could be recovered with improved oil quality, possessing higher levels (> 1.5 fold) of desirable constituents of the oil like citronellal, citronellol, geraniol and geranyl acetate together with a reduced level (> 5 fold) of elemol which is an undesirable component of the citronella oil. Out of the 19 selected somaclones that were evaluated in a replicated trial with the donor parent as control, 5 lines have been further placed under multitocational trials for stability assessment. The scope of sormaclonal variation is discussed in the improvement of vegetatively propagated aromatic grasses that have a narrow genetic base. 相似文献