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1.
臭氧消毒是工厂化水产养殖中水处理的关键技术之一。本文详细介绍了臭氧的物理、化学性质,论述了臭氧制造、水中溶解及水中溶解浓度的检测方法。结合国内外有关水产养殖水处理技术的研究成果与发展趋势,分析了臭氧在工厂化水产养殖水处理中的一些作用和应用特性。介绍了臭氧在工厂化水产养殖中消毒杀菌、氧化有机物、凝聚悬浮物、除臭与除色方面的作用,阐明了臭氧在工厂化水产养殖中的应用前景。  相似文献   

2.
Total ozone production (TOP) from an ozonator, residual ozone concentration (ROC) in water, and the effects of ozone with or without probiotic supplemented feeds on bacterial growth, and shrimp (Penaeus monodon) survival were investigated. Minimal effective ROC to inhibit 3 log units of Vibrio harveyi D331 for 6 h and 2 log units of Bacillus S11 for 9 h was 0.38 g O3/l of ROC from 5-min ozonation. Shrimp postlarvae exposed to 0.34–0.50 mg O3/l ROC (8-h ozonation) caused loss of balance, immobility and destruction of gill lamellar epithelium. In vivo treatment of juvenile P. monodon reared on probiotic feed for 1 month revealed that 0.35 mg O3/l ROC (30-min ozonation) effectively inhibited 3 log units of V. harveyi D331 for 24 h. At this ROC dosage, there was no effect either on shrimp or on intestinal probiotics (Bacillus S11). Shrimp survival from probiotic treatment, coupling with ozonation, increased significantly (P≤0.05) compared with controls.  相似文献   

3.
Denitrification in recirculating systems: Theory and applications   总被引:20,自引:0,他引:20  
Profitability of recirculating systems depends in part on the ability to manage nutrient wastes. Nitrogenous wastes in these systems can be eliminated through nitrifying and denitrifying biofilters. While nitrifying filters are incorporated in most recirculating systems according to well-established protocols, denitrifying filters are still under development. By means of denitrification, oxidized inorganic nitrogen compounds, such as nitrite and nitrate are reduced to elemental nitrogen (N2). The process is conducted by facultative anaerobic microorganisms with electron donors derived from either organic (heterotrophic denitrification) or inorganic sources (autotrophic denitrification). In recirculating systems and traditional wastewater treatment plants, heterotrophic denitrification often is applied using external electron and carbon donors (e.g. carbohydrates, organic alcohols) or endogenous organic donors originating from the waste. In addition to nitrate removal, denitrifying organisms are associated with other processes relevant to water quality control in aquaculture systems. Denitrification raises the alkalinity and, hence, replenishes some of the inorganic carbon lost through nitrification. Organic carbon discharge from recirculating systems is reduced when endogenous carbon sources originating from the fish waste are used to fuel denitrification. In addition to the carbon cycle, denitrifiers also are associated with sulfur and phosphorus cycles in recirculating systems. Orthophosphate uptake by some denitrifiers takes place in excess of their metabolic requirements and may result in a considerable reduction of orthophosphate from the culture water. Finally, autotrophic denitrifiers may prevent the accumulation of toxic sulfide resulting from sulfate reduction in marine recirculating systems. Information on nitrate removal in recirculating systems is limited to studies with small-scale experimental systems. Packed bed reactors supplemented with external carbon sources are used most widely for nitrate removal in these systems. Although studies on the application of denitrification in freshwater and marine recirculating systems were initiated some thirty years ago, a unifying concept for the design and operation of denitrifying biofilters in recirculating systems is lacking.  相似文献   

4.
The high levels of water-reuse in intensive recirculating aquaculture systems (RAS) require an effective water treatment in order to maintain good water quality. In order to reveal the potential and limitations of ozonation for water quality improvement in marine RAS, we tested ozone's ability to remove nitrite, ammonia, yellow substances and total bacterial biomass in seawater, considering aspects such as efficiency, pH-dependency as well as the formation of toxic ozone-produced oxidants (OPO). Our results demonstrate that ozone can be efficiently utilized to simultaneously remove nitrite and yellow substances from process water in RAS without risking the formation of toxic OPO concentrations. Contemporaneously, an effective reduction of bacterial biomass was achieved by ozonation in combination with foam fractionation. In contrast, ammonia is not oxidized by ozone so long as nitrite and yellow substances are present in the water, as the dominant reaction of the ozone-based ammonia-oxidation in seawater requires the previous formation of OPO as intermediates. The oxidation of ammonia in seawater by ozone is basically a bromide-catalyzed reaction with nitrogen gas as end product, enabling an almost complete removal of ammonia-nitrogen from the aquaculture system. Results further show that pH has no effect on the ozone-based ammonia oxidation in seawater. Unlike in freshwater, an effective removal of ammonia even at pH-values as low as 6.5 has been shown to be feasible in seawater. However, as the predominant reaction pathway involves an initial accumulation of OPO to toxic amounts, we consider the ozone-based removal of ammonia in marine RAS as risky for animal health and economically unviable.  相似文献   

5.
Photosynthetic suspended-growth systems in aquaculture   总被引:3,自引:0,他引:3  
Standardized evaluation and rating of biofilters for aquaculture should be assessed in the context of the economic efficiency of ecological services (waste assimilation, nutrient recycling, and internal food production) provided by earthen ponds, and the availability and cost of land, water, and electrical energy resources required to support particular classes of production systems. In photosynthetic suspended-growth systems, water quality control is achieved by a combination of natural and mechanical processes. Natural processes include photosynthesis of oxygen, algal nutrient uptake, coupled nitrification–denitrification, and organic matter oxidation; mechanical processes include aeration and water circulation. Ammonia is controlled by a combination of phytoplankton uptake, nitrification, and immobilization by bacteria. Unlike biofilters for recirculating aquaculture systems, unit processes are combined and are an integral part of the culture unit. The important design and operational considerations for photosynthetic suspended-growth systems include temperature effects, aeration and mixing, quantity and quality of loaded organic matter, and fish water quality tolerance limits. The principle advantages of photosynthetic suspended-growth systems are lower capital costs relative to other recirculating aquaculture systems and increased control over stock management relative to conventional static ponds. The main disadvantage is the relatively low degree of control over water quality and phytoplankton density, metabolism, and community composition relative to other recirculating aquaculture systems. Examples of photosynthetic suspended-growth systems include semi-intensive ponds, intensively aerated outdoor lined ponds, combined intensive–extensive ponds, partitioned aquaculture systems, greenwater tanks, greenwater tanks with solids removal, and greenwater recirculating aquaculture systems.  相似文献   

6.
Total ammonia nitrogen (TAN) concentration is often a key limiting water quality parameter in intensive aquaculture systems. Removing ammonia through biological filtration is thus the first objective in recirculating aquaculture system design. In this study, the performance characteristics of a steady-state nitrification biofilm were explored using a series of reactors. Four nitrification kinetics parameters were estimated using the data collected from the experimental system, including minimum TAN concentration, half saturation constant, maximum TAN removal rate and maximum specific bacterial growth rate. Experimental data showed that a minimum TAN concentration was needed to support a steady-state nitrification biofilm. For the temperature of 27.2°C, the mean minimum TAN concentration was 0.07 mg/l. For a single substrate-limiting factor, the relationship between TAN removal rate (R) and TAN concentration (S) was represented by an empirical equation [R=1859(S−0.07)/(S+1.93)]. The characteristics of nitrite oxidation were also demonstrated by the experiment system. The results of this study will help to better understand the characteristics of nitrification biofilters applied in recirculating aquaculture systems.  相似文献   

7.
The power law in particle size analysis for aquacultural facilities   总被引:1,自引:0,他引:1  
Fine particles tend to accumulate in recirculating aquacultural facilities, which can lead to serious fish health problems. The literature indicated that in many particle systems, the particle size distribution may be accurately represented by a two-parameter, hyperbolic power-law function, dN/dl=Alβ.

The purpose of this paper is to investigate whether this observation holds for aquacultural facilities. Particle size distribution data from four research establishments were examined, using 14 data sets, comprising 11 aquacultural systems, and covering three species of salmonids and one of striped bass. In the data examined, the exponent, β, varied from 2.9 to 4.6, with one sample reaching as high as 6.3. Correlation coefficients varied from 0.998 to 0.975. Larger exponents, indicating a greater number of fine particles, appear to dominate recirculating aquaculture systems.

The results strongly suggest that a hyperbolic distribution is valid for aquaculture systems. The usefulness in this technique is that once a worker has established the exponent from the distribution of particle sizes at any given point in his facility, an estimate of particulate contributions by size to the total particle number, surface area and volume/mass concentration can be obtained.  相似文献   


8.
By keeping tropical shrimp, like Litopenaeus vannamei, in recirculating aquaculture systems (RAS), valuable food for human consumption can be produced sustainable. L. vannamei tolerates low salinities, and therefore, the systems can operate under brackish water conditions. The stabilization of the microbial community in RAS might be difficult under high organic loads, and therefore, water treatment measures like UV irradiation or ozone application are commonly used for bacterial reduction. To investigate the impact of these measures, the effects of UV irradiation and ozone application were studied in small-scale brackish water RAS with a salinity of 15‰ stocked with L. vannamei. UV reactors with 7 and 9 W were used, and by ozonizers with a power of 5–50 mg/hr, the redox potential in the water was adjusted to 350 mV. Ozone had a stabilizing effect on the microbial composition in the water and on biofilms of tank surfaces and shrimp carapaces, prevented an increase of nitrite and accelerated the degradation of nitrate in the water. UV irradiation led to changes in the microbial composition and was less effective in optimizing the chemical water quality. Thus, the use of ozone could be recommended for water treatment in brackish water RAS for shrimp.  相似文献   

9.
This paper describes the performance characteristics of an industrial-scale air-driven rotating biological contactor (RBC) installed in a recirculating aquaculture system (RAS) rearing tilapia at 28 °C. This three-staged RBC system was configured with stages 1 and 2 possessing approximately the same total surface area and stage 3 having approximately 25% smaller. The total surface area provided by the RBC equaled 13,380 m2. Ammonia removal efficiency averaged 31.5% per pass for all systems examined, which equated to an average (± standard deviation) total ammonia nitrogen (TAN) areal removal rate of 0.43 ± 0.16 g/m2/day. First-order ammonia removal rate (K1) constants for stages 1–3 were 2.4, 1.5, and 3.0 h−1, respectively. The nitrite first-order rate constants (K2) were higher, averaging 16.2 h−1 for stage 1, 7.7 h−1 for stage 2, and 9.0 h−1 stage 3. Dissolved organic carbon (DOC) levels decreased an averaged 6.6% per pass across the RBC. Concurrently, increasing influent DOC concentrations decreased ammonia removal efficiency. With respect to dissolved gas conditioning, the RBC system reduced carbon dioxide concentrations approximately 39% as the water flowed through the vessel. The cumulative feed burden – describes the mass of food delivered to the system per unit volume of freshwater added to the system daily – ranged between 5.5 and 7.3 kg feed/m3 of freshwater; however, there was no detectable relationship between the feed loading rate and ammonia oxidation performance.  相似文献   

10.
Water recirculating systems have been used in the shellfish industry for depuration and wet-storage. Knowledge of shellfish excretion characteristics is critical to recirculating system design. In this study, the excretion rate of total ammonia nitrogen (TAN), total Kjeldahl nitrogen (TKN), and 5-day biochemical oxygen demand (BOD5) from Manila clams (Tapes philippinarum) were investigated under both laboratory and commercial conditions. The laboratory tests were conducted under temperatures ranging from 3 to 30°C. The experimental results showed that temperature was a key factor in determining the excretion rate of all the above parameters. The relationship between TAN excretion rate (RTAN) and temperature (T) can be represented by an exponential function (RTAN=0.57×1.25T). For the temperature range between 3 and 20°C, the daily mean excretion rates of TAN, TKN and BOD5 ranged between 1.5–46.1, 4.8–131.0 and 57.4–219.4 mg per kilogram of the clams (wet weight with shell on), respectively. There were linear correlations between TAN, TKN and BOD5 production rates. The data presented in this paper can be used to estimate waste generation from a given shellfish processing operation and to size the waste treatment components for a recirculating depuration (or wet-storage) system.  相似文献   

11.
Profiles of changes in physiological parameters of freshwater giant prawns, Macrobrachium rosenbergii, exposed to various dissolved oxygen (DO) levels of 7.75, 4.75, 2.75, and 1.75 mg l−1 are reported. The parameters involved in osmoregulation and oxygen transport were monitored for a 6-day period. Notable depressions in hemolymph osmolality, Na+, K+, and Cl contents were observed within 24 h after exposure to hypoxia at 2.75 and 1.75 mg O2 l−1, and thereafter remained at rather steady levels, which were significantly lower than those under normoxic conditions (4.75–7.75 mg O2 l−1). The extent of depression of osmotic-related constituents, hemolymph osmolality and Cl in particular, increased with decreased DO.

Oxyhemocyanin constituted 65.46–65.84% of total hemolymph proteins under the various DO levels examined; both hemolymph oxyhemocyanin and proteins showed notable elevations 24 h after exposure to hypoxic conditions, and reached the highest and constant level by 48 h after exposure. The compensatory responses of prawns to reduced O2 were manifested by increased O2 uptake through augmentation of hemocyanin, which results in enhancement of oxygen binding capacity of the hemolymph. In the same period, a significant surge of the respiratory products, PCO2 and HCO3, was also demonstrated 6 h after exposure to hypoxic conditions which resulted in hemolymph alkalosis. These processes likely resulted in an increase in water influx and consequent declines in hemolymph osmolality and ion composition. Furthermore, hyperventilation and respiratory alkalosis, indicated by increased oxyhemocyanin and pH, respectively, were found to be predominant responses of M. rosenbergii to hypoxic stress.  相似文献   


12.
This review deals with the main mechanisms and parameters affecting design and performance of trickling filters in aquaculture. Relationships between nitrification rates and easily accessible process parameters, like bulk phase concentration of TAN, O2, organic matter (COD), nitrite, temperature, HCO3, pH and the hydraulic loading of the trickling filter, are discussed in relation to the design and operation of such filters. Trickling filter design procedures are presented and one of them, a model describing the nitrification performance of trickling filters by plug-flow characteristics, is discussed in greater detail. Finally, practical aspects in relation to filter design and operation are presented.  相似文献   

13.
鲟鱼工厂化循环水养殖系统设计及运行效果   总被引:2,自引:0,他引:2  
针对目前中国淡水工厂化循环水养殖系统建设和运行成本过高,推广应用受到一定程度制约的问题,在自主研发斜管重力滤沉淀装置、内循环流化床反应器、一体化臭氧接触反应器等水净化设备的基础上,通过应用物质平衡相关原理,精确设计、确立不同阶段系统关键运行参数,建立一种高效节能的鲟鱼工厂化循环水养殖系统。通过96 d养殖试验,结果显示,鲟鱼摄食和生长情况正常,养殖密度平均(41.2±2.3)kg/m~3,存活率95.8%,饲料系数1.17。日换水量在5%以下,水质情况良好,氨氮和亚硝酸盐氮后期稳定控制在(0.80±0.21)mg/L和(0.38±0.12)mg/L;系统平均日耗电量为33.3 kW·h,平均产出1kg鲟鱼耗电7.30 kW·h。系统运行具有低能耗、高效率的特点,可为鲟鱼循环水养殖提供技术支撑。  相似文献   

14.
Foam fractionation is often considered an ineffective way of removing organic matter from freshwater due to the low surface tension of the water. There is, however, a lack of studies testing foam fractionation efficiency in replicated freshwater recirculating aquaculture systems (RAS). Foam fractionation can be applied with or without ozone. Ozone is a strong oxidiser previously shown to improve water quality and protein skimmer efficiency. To test the efficiency of foam fractionation and ozonation (20 g O3 kg-1 feed) separately and in combination in freshwater RAS, a two-by-two factorial trial was conducted with each main factor at two levels (applied or not applied). Each treatment combination was carried out in triplicates using 12 replicated pilot scale RAS stocked with juvenile rainbow trout (Oncorhynchus mykiss) and operated at a feed loading of 1.66 kg feed m-3 make-up water. The trial lasted 8 weeks and samples were obtained once a week. Ozone applied by itself significantly reduced the number of particles (83%), bacterial activity (48%) and particulate BOD5 (5-days biochemical oxygen demand; 54%), and increased ultra violet transmittance (UVT; 43%) compared to the untreated control group. Foam fractionation by itself lead to significant reductions in particle numbers and volume (58% and 62%, respectively), turbidity (62%), bacterial activity (54%) and total BOD5 (51%). A combination of both treatments resulted in a significant additional improvement of important water quality variables, including a 75% reduction in total BOD5, 79% reduction in turbidity, 89% reduction in particle numbers and 90% reduction in bacterial activity compared to the control. The removal efficiencies were within the same range as those observed in previous studies conducted with foam fractionators in saltwater systems (with or without ozone), corroborating that foam fractionation may become a useful tool for controlling organic matter build-up and bacterial loads in freshwater RAS.  相似文献   

15.
随着可用水资源的减少,工业化循环水养殖是现代渔业的发展趋势。为了提高工业化循环水养殖的自动化程度,以及将其与物联网更好地结合起来,设计了基于易控的工业化循环水养殖系统。系统采用封闭式循环水养殖工艺,选用微滤机、流化床、低压纯氧混合装置等国内先进的循环水养殖装备构建硬件系统,使用西门子S7-300 PLC和其它智能仪表设备等构建控制系统,通过易控软件作为人机交互平台将各要素进行整合。该系统实现了工业化循环水养殖系统的养殖过程智能控制、养殖水质精准调控和养殖控制物联网化,具备自动化程度高、运行稳定、扩展性强的优点。该系统易于推广,并为将来的福利养殖系统提供了理论依据和基础数据。  相似文献   

16.
为了实现规模化人工养殖小丑鱼(Amphiprioninae),研发了小丑鱼室内循环水养殖设施和技术。1组循环水养殖系统由10个玻璃钢养殖桶和1个水处理玻璃缸及管道系统组成,采用物理过滤、生化过滤、藻板过滤进行循环水处理。1组循环水养殖系统每3个月可养殖产出全长约3.5 cm的商品小丑鱼5 000尾,养殖存活率达80%以上。从2014年至2015年,利用该设施养殖生产出商品小丑鱼10余万尾。和常规的食用海水鱼循环水养殖设施相比,小丑鱼室内循环水养殖系统主要减少了蛋白分离器、气浮机、微滤机等设备,增加了藻板过滤设施。研究表明,小丑鱼室内循环水养殖系统建造成本低、运行能耗低、管理维护简单、水质稳定,可基本实现全封闭循环水养殖,适合进行小丑鱼等海水珊瑚礁观赏鱼类的规模化养殖生产。  相似文献   

17.
The purpose of this study was to develop a mathematical model to assist in the determination of design numbers for color destruction by ozone as a function of feed rate. A mass balance model was developed that takes into account methods of introduction and removal of color, including removal by ozone. Because direct measurement of color mass or concentration is difficult, a representative unit was developed during this study called the color mass equivalent (CME). The CME represents a direct measurement of absorbance at 436 nm, which is a unitless measurement, multiplied by the system volume. The CME is directly proportional to the true concentration of color causing compounds at that wavelength. Once the model was developed, two studies were run to test the model. A 1,500-L recirculating fish system was set up with a 57-L bead filter and six 1-L fluidized sand beds. Approximately 45 kg of channel catfish Ictalurus puncfatus were placed in the system and fed 0.25 kg of 32% protein feed per day (0.55% body wt.). Once the system reached stable conditions, the system was flushed with clean water and samples were taken to estimate the accumulation rate of color in the system. After 5 wk, an ozone unit was activated and samples were taken to determine the destruction rate for color. Once an accumulation rate had been determined, a second study was performed to corroborate this value. The same ozone unit was installed on a 5,000-L recirculating fish system being fed 0.49 kg per day with a 171-L bead filter. Samples were taken to determine the destruction rate for color. The accumulation rate for color was 12.6 CME/kg feed and the destruction rates were 1.7 CME/g O3 in the first study and 0.82 CMWg O3 in the second study. This calculates to a range of 7 to 15 g O3/kg feed to remove the color produced by the feed.  相似文献   

18.
本文通过在循环水养殖系统中添加不同浓度的臭氧,研究其对循环水养殖系统生物膜活性及其净化效能的影响.结果显示,当氧化还原电位(ORP)小于450 mV时,氨氮的去除率随着臭氧浓度升高而升高,最高去除率达39.9%,亚硝酸盐氮的平均去除率为28.2%,生物膜菌群的平均存活率为88.1%,生物膜对养殖水体氨氮和亚硝酸盐氮的处理效果良好;当氧化还原电位为500 mV时,经过臭氧24 h处理,氨氮和亚硝酸盐氮的去除率分别由36.5%、28.1%降到12.2%、8.4%,而臭氧4h处理后,生物膜对氨氮和亚硝酸盐氮的去除率分别由47.5%、32.1%降到5.0%、3.3%,水处理效果明显下降,生物膜菌群存活率由88.1%降到31.5%.由此可见臭氧添加浓度对生物膜及净化效能有重大影响.综合试验结果和分析评估,建议封闭循环水养殖系统的臭氧添加量以控制生物滤池内的氧化还原电位低于400 mV为宜,可保证循环水系统的安全性和经济性.  相似文献   

19.
As part of a larger study of micro-particles in a recirculating, cold water aquaculture system for juvenile Atlantic salmon (Salmo salar), particle size analyses were carried out on the culture and makeup waters over several months followed by samples of feed stirred in water. The particle size distributions followed the power law described in [Aquacult. Eng. 19 (1999): 259] but with a superimposed spike at around 4 μm in the culture water, which was not present in make-up waters. A similar spike appeared in the feed samples indicating that the spike had a feed origin. While the absolute value of the power law exponent (designated β, or the Beta value) was in the range of 2.6–3.6, consistent with aquacultural waters, the build-up of fine particles, expected in a recirculating system with time and which would have been indicated by an increase in Beta value to above 4, did not occur due to a system high water turn-over rate flushing much of the finer particles. There is a particle counts increase occurring across the degasser/oxygenator that needs further investigation.  相似文献   

20.
The potential of using Bannister's linear equation (kt=kw+kcc) (where kt is the overall light extinction coefficient, kw represents the non-phytoplankton light extinction, kc is the specific light extinction coefficient due to chlorophyll a (chla), c is the chla concentration, and kcc represents the light extinction due to chla) to partition sources of turbidity in Secchi disk visibility (SDV) measurements in aquaculture ponds was evaluated. Eight data sets from five sites around the world were used in the study. Chlorophyll a data were regressed against the overall light extinction coefficient determined from SDV measurements. The relationship between chla and overall light extinction coefficient was linear for seven of the eight data sets. The contribution of non-phytoplankton turbidity to SDV measurements was estimated by the intercept of the linear regression line (equivalent to kw). The values obtained (range=3.61–8.91 m−1) were variable and unpredictable between replicate ponds at all sites, but did not vary significantly over time (P<0.05). Because chla concentration serves as an indicator of phytoplankton concentration, the contribution of phytoplankton turbidity to SDV measurements was estimated by the slope of the linear regression line (equivalent to kc) multiplied by the chla concentration. The slope of the regression line (0.014±0.006 m−1 (mg m−3) −1) was similar to values reported for natural freshwater systems. The partitioned light extinction coefficients and chla concentrations were also used to determine the threshold chla concentration above which SDV measurements are determined primarily by chla. The threshold chla concentrations (177–980 mg m−3) above which phytoplankton biomass becomes the primary determinant of SDV were higher than observed chla concentrations. The results indicate that Bannister's linear equation can generally be used to partition and quantify the sources of turbidity in aquaculture ponds. The results also suggest that the contribution of non-phytoplankton turbidity to SDV measurements in fertilized and fed aquaculture ponds can be more important than phytoplankton turbidity.  相似文献   

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