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1.
Twenty-one clones of the peach-potato aphid, Myzus persicae (Sulzer), carrying various combinations of known mechanisms of insecticide resistance were assessed for their response to the new pyridine azomethine compound, pymetrozine, in leaf-dip bioassays. Pymetrozine was also applied as a foliar spray to populations of four different UK M persicae clones on potato plants in field-simulator chambers. Neither study showed any evidence of cross-resistance to this compound. Pymetrozine, used in conjunction with other effective aphicides, such as pirimicarb and imidacloprid, can therefore play an important role in insecticide resistance-management strategies for M persicae.  相似文献   

2.
吡虫啉及其复配剂不同施药方法对油菜蚜虫控制效果   总被引:4,自引:0,他引:4  
2006-2009年,采用拌种和播种沟施药的方法,于河南郑州研究了新烟碱类杀虫剂吡虫啉及吡虫啉.辛硫磷复配剂对油菜蚜虫的全程控制效果。小区试验结果表明,用吡虫啉有效成分5g/kg种子拌种仅能有效控制苗期油菜蚜虫的危害,防治效果为90.79%~100%,对中后期油菜蚜虫控制效果不明显;用吡虫啉600g/hm2及吡虫啉.辛硫磷复配剂1320g/hm2采用播种沟施药对油菜蚜虫的防治效果分别为91.82%~94.49%和90.68%~95.34%;试验示范验证防治效果在92%以上,持效期长达7个月以上,可以达到一次用药控制油菜整个生长期蚜虫为害的效果,减少了用药次数,此方法简单易行、保护环境、保护天敌,是一种简化高效的具有推广价值的油菜蚜虫可持续控制技术。  相似文献   

3.
多数马铃薯病毒可以借助蚜虫传播, 并通过块茎世代积累, 导致马铃薯种性退化, 严重影响块茎的产量和品质?为了筛选新型?环保的马铃薯病毒病防治药剂, 本研究通过3个季节的田间试验, 对矿物油?维生素B1和杀虫剂吡虫啉在防治马铃薯病毒病中的效果进行了评价?结果表明, 通过马铃薯出苗后间隔10 d连续3次喷施, 矿物油能够控制马铃薯卷叶病(potato leaf-roll virus, PLRV)的发生, 对马铃薯M病毒(potato virus M, PVM)和马铃薯S病毒(potato virus S, PVS)的平均防效也分别达到66.72%和70.40%, 但对马铃薯Y病毒(potato virus Y, PVY)和马铃薯A病毒(potato virus A, PVA)在不同的年份和季节的防效不稳定, 平均防效为27.34%和65.02%?维生素B1对PLRV?PVM和PVS的防效也比较明显, 分别达83.36%?83.33%和73.32%, 而对PVY同样防效不稳定, 对PVA防效不明显?杀虫剂吡虫啉对PLRV?PVS和PVM的防效也不稳定, 且对PVY和PVA的防效均不显著?本研究中马铃薯X病毒(potato virus X, PVX)发生频率极低, 未进行病毒病的防效比较?综上所述, 矿物油和维生素B1对马铃薯主要病毒病的综合防效较吡虫啉好, 同时它们的增产效果更明显, 产投比高于化学药剂, 值得推广?  相似文献   

4.
The inhibitory effect on the spread of viruses in lilies viz., tulip breaking virus (TBV; nonpersistently aphid-borne, potyvirus,) lily symptomless virus (LSV; non-persistently alphidborne, carlavirus), and lily virus X (LVX; potexvirus of unknown etiology), was studied of brands of mineral oil (Luxan oil H and Duphar-7E oil) and synthetic pyrethroid insecticides (l-cyhalothrin and deltamethrin), and a nematicide (aldicarb) in crops in which virus-infected plants were present as virus sources. The spread of TBV and LSV were controlled by sprays of mineral oil and insecticide, while that of LSV was also limited by the soil-applied nematicide. The spread of LVX was reduced by the insecticides and, not effectively by the mineral-oil spraying, by which data the mode of transmission may be presumed to be by an insect in the persistent or semi-persistent manner.Mixtures of mineral oil and pyrethroid were more effective in the reduction of spread of TBV and LSV than either components tested alone. The mineral oil was the most effective component in the mixtures in which pyrethroid added a slight extra effect. The addition of pyrethroid did not mask either the lower efficacy of the oil brand Duphar-7E oil, or the diminished inhibitory effect of low dosages of oil. The normal rate of mineral oil gave similar control to that of a mixture of mineral oil at half rate plus the pyrethroid at full dosage. Low rates of oil, or even synthetic pyrethroids alone may be used on cultivars which suffer of the loss of bulb weight by the use of normal or decreased rates of oil. Weekly sprays were more effective than fortnightly sprays. The rate of control by the weekly sprays ranged between 90 and 95% for Luxan oil H at half dosage plus the full rate of pyrethroid. Weekly sprayed synthetic pyrethroids alone onto the virus sources and the plants to be infected gave 60–70% control. The weight ratios tended to be slightly reduced if the half dosage of the efficient Luxan oil H was used. Factors which affect the control of the air-borne field spread of viruses by mineral oils and synthetic pyrethroid insecticides in lilies are discussed.  相似文献   

5.
6.
BACKGROUND: The objectives of this work were to determine if the control of potato virus Y (PVY, genus Potyvirus, family Potyviridae) in seed potato could be improved by combining border crops and mineral oil sprays, and if the border crop acts as a barrier or a virus sink. RESULTS: Field tests over 3 years confirmed that mineral oils alone are an effective barrier to PVY, and showed that borders alone act as a PVY sink. Combining the familiar mineral oil and the more recent crop border methods was almost twice as effective in reducing PVY incidence as either one used alone. The combination provided consistently high PVY control compared with the variable and often lower level of control by either method alone. The contribution of the oil to PVY reduction was similar whether it was applied to the border, the center seed plot, or both. Oil application to the border alone should not affect efficacy and would help keep control costs down. CONCLUSION: Combining border and oil provided the best reduction in PVY incidence 3 years out of 3, providing producers with a tool to reduce year‐to‐year variation in the effectiveness of crop borders or oil sprays used separately. © Her Majesty the Queen in Right of Canada, as represented by the Minister of Agriculture and Agri‐Food Canada. Published by John Wiley and Sons, Ltd.  相似文献   

7.
A range of insecticides was applied at recommended application rates against populations of Myzus persicae (Sulzer) carrying various combinations of three insecticide resistance mechanisms (carboxylesterase-based metabolic resistance and two target-site mechanisms, known as MACE and kdr), supported on either Chinese cabbage or potatoes in field simulator cages. Patterns of response were similar on both host species. MACE conferred extreme resistance to pirimicarb and triazamate (dimethylcarbamate insecticides). The kdr mechanism was associated with resistance to lambda-cyhalothrin, cypermethrin and deltamethrin (pyrethroid insecticides). A mixture of pirimicarb plus lambda-cyhalothrin was only effective against M persicae not carrying kdr or carrying kdr and low carboxylesterase-based resistance. None of the insecticides tested was effective against M persicae carrying both MACE and kdr resistance. The implications of these findings for the formulation of control strategies, based on regular monitoring of resistance genotype frequencies, are discussed.  相似文献   

8.
三种杀虫剂对麦田蚜虫和捕食性天敌群落的影响   总被引:4,自引:1,他引:4  
以2种选择性杀虫剂吡虫啉、抗蚜威和一种广谱性杀虫剂氧化乐果为试验材料,研究了选择性杀虫剂对麦田蚜虫和捕食性天敌群落结构特征的影响。结果表明,施用选择性杀虫剂的小区在农药持效期内麦蚜群落的多样性指数和均匀度低于空白对照区,高于氧化乐果处理区,而优势度和优势集中度高于空白对照区,低于氧化乐果处理区;捕食性天敌群落的多样性指数低于空白对照区,高于氧化乐果处理区,优势度和优势集中度高于空白对照区,低于氧化乐果处理区。选择性杀虫剂处理区瓢蚜比小于空白对照区和氧化乐果处理区,且有效期长达15-16d。根据害虫和天敌发生的特点及选择性杀虫剂的特性,提出了麦蚜的有效防治措施,即在麦蚜为害关键时期,每667m^2用吡虫啉1g(ai)或抗蚜威2.5g(ai)喷施1次,再利用自然天敌调控作用,即可有效控制麦蚜。  相似文献   

9.
Potato spindle tuber viroid (PSTVd) was transmitted by Myzus persicae to Physalis floridana from P. floridana plants that also were infected with potato leafroll luteovirus (PLRV), whereas it was not transmitted by aphids from plants infected with PSTVd alone. Dot-blot hybridisation was used to detect PSTVd. The results indicate that PLRV can assist PSTVd in its transmission by M. persicae. Doubly infected, aphid-inoculated P. floridana plants from the previous experiment were used as the source plants in aphid transmission tests to the tomato cv. Rutgers, P. floridana and Datura stramonium. PSTVd was detected in 17 of 30 plants of tomato. The viroid was not detected by dot-blotting in any plant of P. floridana and D. stramonium in this experiment, but it was recovered from some plants by sap inoculation of the Rutgers plants. Treatment with RNase A of PLRV preparations purified from doubly infected plants indicated that PSTVd was encapsidated by PLRV particles.  相似文献   

10.
柑橘木虱防控药剂筛选及矿物油对其增效作用评价   总被引:1,自引:0,他引:1  
为了筛选柑橘木虱高效防控药剂,提高药剂防治效果,本文探索了 10种杀虫剂对柑橘木虱的田间防效及矿物油对杀虫剂的增效作用.结果表明:对柑橘木虱成虫,22%噻虫·高氯氟悬乳剂(SE)、70%吡虫啉可湿性粉剂(WP)、6%联菊·啶虫脒微乳剂(ME)和5%高效氯氟氰菊酯ME的速效性好,药后1 d防效94.61%~100%;22...  相似文献   

11.
BACKGROUND: Pest resurgence following a pesticide application may occur owing to a stimulatory (hormetic) response to sublethal insecticide concentrations. The objective of the present study was to examine the potential for a greenhouse‐derived red clone of Myzus persicae to exhibit resurgence owing to a hormetic response following a systemic imidacloprid treatment in a bell pepper greenhouse. RESULTS: No differences in mortality and fecundity were observed among apterous adults exposed to sublethal imidacloprid concentrations on excised pepper leaves fed aqueous solutions of imidacloprid. Survival of first‐generation progeny was negatively affected by imidacloprid exposure, yet surviving progeny exhibited no differences in development rates or fecundity from progeny of adults unexposed to imidacloprid. Aphid mortality declined most rapidly in clip cages on pepper leaves at the top of the pepper canopy as compared with leaves present at the middle or bottom of the pepper canopy. CONCLUSION: Imidacloprid decays rapidly in mature pepper plants, resulting in sublethal concentrations in the upper canopy in as little as 4 weeks. Sublethal insecticide concentrations have been implicated in the resurgence of pest populations; however, exposure to sublethal doses of imidacloprid are unlikely to result in pesticide‐induced resurgence of the M. persicae aphid clone examined in this study. Copyright © 2011 Society of Chemical Industry  相似文献   

12.
Thirteen fractions were obtained by distillation of the mineral oil Bayol 50 (ESSO 2214) and these were tested for their ability to inhibit the transmission of potato virus Y to red pepper. The less viscous fractions <8 cSt at 37°C (52 SUS) were shown to be less active, the more viscous fractions were more active than Bayol 50 with a viscosity of 8 cSt at 37°C. Sunoco 7E, a mineral oil with a viscosity of 14.2 cSt at 37°C (74 SUS) was shown to be significantly more active than Bayol 50. The difference in emulgator had no influence on these results. We, therefore, recommed to choose mineral oils with a viscosity of at least 12 cSt at 37°C (=66 SUS) for future work on the inhibition of the transmission of stylet-borne viruses.Samenvatting Door destillatie van de minerale olie Bayol 50 (ESSO 2214) werden 13 fracties verkregen die getoetst werden op hun vermogen de overdracht van aardappelvirus Y naar paprika te verhinderen. De minder viskeuze fracties, <8 cSt bij 37°C (52 SUS), bleken minder, de hoger viskeuze fracties meer activiteit te bezitten dan Bayol 50 zelf. Sunoco 7E, een minerale olie met een viscositeit van 14,2 cSt bij 37°C (74 SUS) bleek significant actiever dan Bayol 50 met een viscositeit van 8 cSt bij 37°C (52 SUS). Een verschil in emulgator had geen invloed op deze resultaten. Op grond van deze gegevens is het aan te bevelen geen minerale olie te gebruiken met een viscositeit lager dan 12 cSt bij 37°C (66 SUS) indien men de overdracht van een nonpersistent virus zo effectief mogelijk wenst te verhinderen.  相似文献   

13.
The effects of five economically important vegetable (seed) oils, peanut, cottonseed, castor, soybean and sunflower, on adult and immature stages of the sweetpotato whitefly(Bemisia tabaci (Gennadius) [Hemiptera: Aleyrodidae]) were studied in the laboratory. Irrespective of origin, oil residues had similar effects on immature stages and adult mortality, and on settling and oviposition deterrence. Trends for 5-h settling, 24-h oviposition and 24-h survival of adults on treated plants were similar for oils applied at the same concentration range. Settling deterrence was strong enough to cause adult death due to starvation or dehydration under no-choice conditions, and caused greater mortality than direct physical toxicity; similar results were obtained when adult survival on oil residues was compared to survival of starved adults. Adults recovered when transferred from oil-treated to untreated plants. All oils produced similar effects when sprayed on immatures; the egg and fourth instar (pupa) were the most tolerant stages. The oil concentration required for significant effects on whitefly mortality and behavior was relatively high (>0.3%) compared with published efficacy data for synthetic pesticides, and varied to some extent with oil origin. All tested oils were active as direct and residual larvicides. Peanut oil was the most effective for all tested effects, followed by cottonseed oil, which was significantly less effective than peanut and castor oils when applied-directly to eggs. Only by this latter parameter was castor oil more effective than cottonseed oil and similar to peanut oil. As direct sprays to larvae, soybean and sunflower oils resembled castor oils, but their residues were less effective against all stages.  相似文献   

14.
Experiments were done on commercial potato crops in the UK to investigate the effect of different insecticide sequences on the control and insecticide resistance status of Myzus persicae (Sulzer). The work was done to provide field validation of similar laboratory studies done in 'field simulators'. To ensure adequate aphid populations and to influence the initial resistance status of the aphid population, cultured M. persicae from a clone of known resistance status (esterase R1, kdr heterozygote, non-MACE (modified acetylcholinesterase)) were inoculated into both experiments. Two-spray programmes starting with lambda-cyhalothrin (a pyrethroid insecticide) gave poor control in comparison with programmes starting with pirimicarb (a carbamate insecticide) or pirimicarb-containing mixtures. This concurred closely with the results obtained from single applications in field simulator studies. Treatment sequences containing pymetrozine (a pyridine azomethine insecticide) were also effective, though slower-acting. This again concurs with field simulator studies. The proportions of aphids carrying different resistance mechanisms were largely unaffected by treatment in these experiments. The implications of these results for field control strategies are discussed.  相似文献   

15.
Laboratory bioassays applying the neonicotinoid insecticides imidacloprid, acetamiprid and nitenpyram against clones of the peach-potato aphid Myzus persicae (Sulzer) demonstrated that these compounds effectively circumvent the known carboxylesterase, modified acetylcholinesterase (MACE) and knock-down (kdr) insecticide resistance mechanisms in this species. However, some clones showed cross-tolerance (up to 18-fold) of these compounds relative to susceptible standards. A survey assessing the frequency of neonicotinoid tolerance in M persicae in the UK, based on samples collected from the field and glasshouses between 1997 and 2000, showed that such tolerance is still rare. Experiments on neonicotinoid-susceptible and -tolerant populations of M persicae under simulated field conditions in the laboratory showed that, although the latter were well controlled by imidacloprid applied at recommended application rates, they were more likely to survive and reproduce when this compound was applied at lower concentrations. Such conditions are probably periodically present in imidacloprid-treated field and glasshouse crops. Selection favouring tolerant forms of M persicae could lead to increases in their frequency and the evolution of more potent resistance to neonicotinoids.  相似文献   

16.
为研究不同种类植物油对刺糖多孢菌生长及其合成多杀菌素能力的影响,探索提高多杀菌素产量的方法,在发酵培养基中分别添加葵花油、花生油、大豆油、芝麻油、橄榄油和菜籽油,研究了其对菌体生长、脂肪酶活性和多杀菌素产量的影响,并利用RT-PCR对脂肪酶基因及多杀菌素合成相关基因的转录水平进行分析。结果表明:6种供试植物油对菌体生长和多杀菌素产量的影响程度不同,依次为菜籽油橄榄油花生油芝麻油葵花油大豆油,其中菜籽油有利于诱导脂肪酶的表达、延缓菌体的衰亡和延长产素期,脂肪酶活力、菌体生物量和多杀菌素产量分别提高310.09%、8.97%和33.94%;脂肪酶基因和多杀菌素合成基因的转录强度也有明显提高。因此,菜籽油是其最佳的辅助性脂类碳源。  相似文献   

17.
This study examines the effects of a vegetable fungicide on sugar beet powdery mildew (Erysiphe betae) and cucumber powdery mildew (Erysiphe cichoracearum). The formulations consisting of a dispersion of Brassicaceae meal in vegetable or mineral oils on infected leaves of sugar beet, reared in the greenhouse, and of musk melons cultivated under plastic tunnels, were tested in comparison to each oil taken separately. Both formulations containing Brassicaceae meals, caused 94% of conidia to be distorted while for the untreated group only 2% were distorted. Furthermore, the leaf area infected by E. betae was 56% for untreated plants and 2.7 and 9.9% respectively, for plants treated with meal containing mineral and vegetable oil. Vegetable oil considered separately or with Brassicaceae meals showed no phytotoxicity, while the formulations based on mineral oil showed a significantly lower fresh and dry weight on tomato plants. The low level or absence of phytotoxicity of plants treated with vegetable oil formulations suggests that to improve the efficacy of powdery mildew control, they could be used mixed with sulphur. The efficiency of the vegetable formulations in the powdery mildew control observed during these trials encourages further investigation on other parasitic fungi and foliar pathogens.  相似文献   

18.
采用微量滴度酶标板法测定了吡虫啉亚致死剂量对桃蚜乙酰胆碱酯酶(AChE)的影响。结果表明,用不同亚致死剂量吡虫啉处理桃蚜后AChE的活力明显降低,表现为抑制作用,且不同剂量间AChE的活力差异显著;酶动力学分析表明,AChE的Km值显著降低,而Vmax显著升高,表明吡虫啉亚致死剂量处理桃蚜后AChE与底物的亲和力明显增强,且最大反应速度显著加快。用LC10的吡虫啉处理桃蚜后AChE的活力随时间的延长而变化,且表现为明显的抑制作用;AChE的Km随时间的变化表现为先升高后降低的趋势,但Vmax均升高。此外研究了LC10的吡虫啉处理桃蚜后AChE在不同亚细胞层的分布特征,结果表明,AChE比活力的高低次序依次为:微粒体>细胞核与细胞碎片>线粒体>细胞质液;吡虫啉亚致死剂量处理后虽然没有影响AChE在亚细胞中的分布,但是却改变了对底物的亲和力和最大反应速度。  相似文献   

19.
The abiogenic and biosynthetic origins of mineral and vegetable oils are contrasted and the importance of soybean, palm, rape seed and sunflower as the major source of fatty acids is highlighted. The cheaper oils derived from animal sources also provide fatty acids. The extraction and refining processes for vegetable oils are explained, the composition of typical vegetable oils is discussed and the importance of the glyceride structure to the physical properties of the oils is emphasised. Stability, volatility and viscosity of vegetable oils are considered. Production of fatty acids by fat-splitting methods and the formation of methyl esters by inter-esterification are outlined. Mineral oil compositions are detailed and their properties explained.  相似文献   

20.
In a study aimed at finding environmentally benign adjuvants for glyphosate, ethoxylates of rapeseed oil and of methylated rapeseed oil were synthesized, with ethylene oxide (EO) content up to 40 and 8 respectively. They had less influence on spray retention by barley shoots than ethoxylated (15 EO) tallow amine (ETA). At 10 g L(-1), ethoxylated rapeseed oil with 30 or 40 EO and ethoxylated methylated rapeseed oil with 6 or 8 EO promoted glyphosate uptake by barley leaves to a greater extent than ETA at the same concentration. However, uptake rates were similar when the concentration was lowered to 2.8 and 3.1 g L(-1) for rapeseed oil derivatives and ETA respectively. In the case of ethoxylated methylated rapeseed oil with 8 EO (MeOil-8), glyphosate uptake increased when MeOil-8 concentration was raised from 5 to 10 g L(-1). In bioassays under controlled conditions, ethoxylated rapeseed oil with 40 EO (Oil-40) and MeOil-8 were slightly less effective than ETA in favouring the efficacy of glyphosate on barley. The same was found on ryegrass. However, both rapeseed oil derivatives compared well with glyphosate formulants such as ethoxylated diethylamine and alkyl ethoxy phosphate. In one field experiment, the efficacy of glyphosate in the presence of Oil-40, MeOil-8 or ETA was comparable with that of a commercial formulation. In another trial, MeOil-8 was as effective as ETA, but Oil-40 performed less well. It is concluded that ethoxylates of rapeseed oil and of methylated rapeseed oil are a promising chemistry for glyphosate adjuvants, provided that their ethylene oxide content is high.  相似文献   

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