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1.
The efficacy of three methods of applying ethanol to prevent storage decay was tested on two cultivars of table grapes, ‘Superior’ and ‘Thompson Seedless’. Ethanol was applied by: (1) dipping grapes in 50% ethanol for 10 s followed by air drying before packaging; (2) placing a container with a wick and 4 or 8 ml ethanol/kg grapes inside the package; (3) applying 4 or 8 ml ethanol/kg grapes to paper and placing this paper above the grapes in the package. The grapes were stored for 6 or 8 weeks at 0 °C and assessed after an additional 3 days at 20 °C. All methods of application controlled decay as well as or better than a SO2-releasing pad. The ethanol impregnated paper caused high levels of berry browning, perhaps because of high levels of acetaldehyde inside the package. However, the taste of the berries was not impaired by any of the ethanol applications. The taste of ‘Thompson Seedless’ grapes stored for 8 weeks in modified atmosphere storage was affected by CO2 levels above 7%. Some methods of applying ethanol used here show promise as alternatives to SO2 to prevent decay of grapes during storage while maintaining fruit quality.  相似文献   

2.
‘Superior seedless’ table grapes were stored for 7 days at 0 °C followed by 4 days at 8 °C + 2 days at 20 °C under modified atmosphere packaging (MAP). Two polypropylene films (PP) were used to generate the MAP, the micro-perforated PP-30 and an oriented PP (OPP). The OPP film was applied with and without fungicide (10 μL of trans-2-hexenal or 0.4 g Na2S2O5 kg−1). As control a macro-perforated PP was used. PP-30 packages reached the lowest O2 and the highest CO2 levels. Control clusters showed the highest weight losses and decay while almost no losses occurred under MAP treatments. No changes in softness, skin and/or pulp browning, or cluster shatter were found. After shelf life MAP-treated clusters showed slight to moderate stem browning, except under SO2 where practically no browning occurred while control clusters showed an extreme stem browning. After shelf life, MAP treatments showed good visual appearance and crunchiness, while control fruits were unmarketable. No off-flavors were detected for MAP treatments except for hexenal-treated berries. No remarkable changes for color, firmness, soluble solids content, pH, titratable acidity and maturity index were detected. Total sugars content at harvest was 200 g L−1 and only slight decreases were found after shelf life for most treatments. Total organic acids content at harvest was 15.4 mg 100 mL−1, which remained quite constant after cold storage and shelf life. The main phenolic compounds were flavan-3-ols (over 85% from the total content), hydroxycinnamic acid derivatives and flavonols, whose total amount at harvest was 140 mg kg−1 in a fresh weight basis. After shelf life only slight decreases in total phenolics occurred in all treatments. As a main conclusion, SO2-free MAP kept the overall quality of clusters close to that at harvest, with few differences when SO2 was added.  相似文献   

3.
Experiments of initial hermetic sealing using high barrier film were carried out on ‘Kyoho’ grapes (Vitis vinifera L. × V. Labrusca L. cv. Kyoho) in the 2008 and 2009 fruit seasons, to investigate their potential to enhance quality and extend storage life of the fruit. In the 2008 season, grapes were packaged in high barrier film bags for 1, 2, 3, 4 and 5 weeks, and a modified atmosphere (MA) of low oxygen and high carbon dioxide was formed after sealing. After packaging, fruit were removed from bags and stored in air for up to 90 d at 0 °C. In the 2009 season, grapes were packaged in perforated bags, or in high barrier film bags for 2 weeks and subsequently perforated bags to avoid further anoxia and excessive CO2 accumulation. After treatment, fruit were stored for up to 90 d at 0 °C, followed by shelf-life at 20 °C for 7 d. Non-packaging air storage was used as a control in both seasons. Fruit quality attributes including soluble solids, titratable acidity, stem browning, berry drop and decay incidence were measured. The results indicated that short-term initial MAP (≤2 weeks) had potential for improving appearance of bunches and maintaining the quality of berries during long-term storage, and significantly reduced quality deterioration. Stems were greener and berry drop and decay incidence were more effectively controlled when fruit were sealed in high barrier film bags for 2 weeks and the bags were subsequently perforated.  相似文献   

4.
Table grapes have high market value in international markets due to their attractive taste and high antioxidant content. However, their market potential is limited by losses due to Botrytis cinerea Pers. Fr. Cinnamon leaf oil (CLO) is a natural fungicidal and antioxidant agent that can be used to avoid postharvest losses due to B. cinerea Pers. Fr. and to increase the antioxidant levels of this produce. CLO was applied to grapes as water emulsions (0, 0.5, 2.5, and 5 g L−1), as vapors (0, 0.196, 0.392, and 0.588 g L−1), or as a chemical incorporated into pectin coatings (0 and 36.1 g L−1). Afterwards, berries were stored at 10 °C for 15 d and were evaluated periodically for the fungal decay index, the total phenolic and flavonoid contents and the antioxidant activity using the Trolox equivalent antioxidant capacity and DPPH radical inhibition methods. The odor acceptability of the treated berries was evaluated after 10 d of storage. The CLO emulsion (5 g L−1) significantly reduced the fungal decay without affecting the antioxidant properties of the berries. The application of CLO as a vapor was more effective according to the evaluated parameters than the emulsions; all tested concentrations inhibited fungal decay and increased the flavonoid content and antioxidant activity. When CLO was incorporated into the pectin, no fungal decay appeared, and the highest antioxidant activity was observed after 15 d of storage. Additionally, all treatments, except the emulsion treatment, increased the odor acceptability of the treated berries compared to the control berries. From this study, it can be concluded that CLO as vapors or coatings can be used to control decay and increase the antioxidant health benefits of grapes due to CLO's antifungal and antioxidant properties.  相似文献   

5.
To control postharvest decay, table grapes are commercially fumigated with sulfur dioxide. We evaluated ozone (O3) fumigation with up to 10,000 μL L?1 of ozone for up to 2 h to control postharvest gray mold of table grapes caused by Botrytis cinerea. Fumigation for 1 h with 2500 or 5000 μL L?1 of ozone were equal in effectiveness. Both treatments reduced postharvest gray mold among inoculated ‘Thompson Seedless’ grapes by approximately 50% when the grapes were examined after storage for 7 d at 15 °C following fumigation. In a similar experiment, ‘Redglobe’ grapes were stored for 28 d at 0.5 °C following fumigation for 1 h with 2500 or 5000 μL L?1 of ozone. Both treatments were equal in effectiveness, but inferior to fumigation with 10,000 μL L?1. Ozone was effective when grapes were inoculated and incubated at 15 °C up to 24 h before fumigation. The cluster rachis sustained minor injuries in some tests, but berries were never harmed. Ozone was applied in three combinations of time and ozone concentration (10,000 μL L?1 for 30 min, 5000 μL L?1 for 1 h, and 2500 μL L?1 for 2 h) where each had a constant concentration × time product (c × t) of 5000 μL L?1 × h. The effectiveness of each combination was similar. The incidence of gray mold was reduced by approximately 50% among naturally inoculated, organically grown ‘Autumn Seedless’ and ‘Black Seedless’ table grapes, and by 65% among ‘Redglobe’ table grapes, when they were fumigated with 5000 μL L?1 ozone for 60 min in a commercial ozone chamber and stored for 6 weeks at 0.5 °C. Residues of fenhexamid, cyprodinil, pyrimethanil, and pyraclostrobin were reduced by 68.5, 75.4, 83.7, and 100.0%, respectively, after a single fumigation of table grapes with 10,000 μL L?1 ozone for 1 h. Residues of iprodione and boscalid were not significantly reduced. Ozone is unlikely to replace sulfur dioxide treatments in conventional grape production unless its efficacy is improved, but it could be an acceptable technology to use with grapes marketed under “organic” classification, where the use of SO2 is prohibited, or if SO2 use were to be discontinued.  相似文献   

6.
After three months storage at 0.5 °C one quarter of a lot of ‘Anjou’ pears (Pyrus communis L.) were treated with 1 μL L?1 of 1-methylcyclopropane (1-MCP) for 8 h at 20 °C and three quarters of the fruit were left untreated at 20 °C for the same time. Treated and untreated pears were then sliced, dipped in a commercial anti-browning solution and packaged in modified atmospheric bags. Packages, containing slices from 1-MCP treated fruit, were labelled as MCP1. Slices from two thirds of the untreated fruit had one of two secondary treatments applied: (1) multi-functional co-release sachets added to the package at the time of sealing (NT), or (2) an injection of 1-MCP to sealed packages to achieve a final concentration of 1 μL L?1 (MCP2). The last third of the slices from the untreated lot of pears were sealed into packages with no further treatment (CK). The packages were kept at 5 °C. In-package ethylene concentrations were significantly lower for the NT treated slices. NT also significantly delayed and reduced net oxygen consumption in the package headspace compared with other treatments. The NT treatment also reduced incidence of browning induced by enzymes of microbial origin, termed secondary browning (SB), and better maintained the measured juiciness of slices. In contrast, the CK, MCP1 and MCP2 treatments showed a more rapid appearance and severity of SB. Slices in packages treated with NT retained higher tissue levels of butyl, hexyl and pentyl acetate, 6-methyl-5-hepten-2-one, butanol and hexanol during storage than any of the other three treatments.  相似文献   

7.
Postharvest diseases limit the storage period and market life of fresh figs (Ficus carica L.). The objective of this work was to determine the effect of sulfur dioxide (SO2) applied by fumigation and/or by dual release SO2 generating pads on postharvest decay and quality retention of ‘Black Mission’ and ‘Brown Turkey’ (dark skin), and ‘Kadota’ and ‘Sierra’ (green skin) figs. A protocol for the computer-controlled application of gaseous SO2 has been developed which allows the application of very low specific concentration × time products of SO2 and simultaneous monitoring of the application progress. In vitro tests with important fungal, yeast and bacterial postharvest pathogens plated on Petri dishes and exposed to a SO2 concentration × time product (C × t) of 100 (μL/L) h at different temperatures showed fewer survived at 20 °C than at 0 °C. Therefore, fumigations were carried out at 20 °C in the rest of the experiments. The evaluation of different SO2 concentration × time products showed that a product of 25 (μL/L) h provided the best compromise between decay control and fruit injury. The performance of SO2 fumigations on warm or cold fruit, its combination with SO2 generating pads, and the use of repeated fumigations during cold storage were also evaluated. All the SO2 treatments tested reduced the percentage of decay, extending the market life of fresh figs. However, in some cases, the use of SO2 generating pads increased the incidence of skin bleaching. Fumigation of warm fruit at 25 (μL/L) h of SO2 reduced populations of Alternaria and Rhizopus spp. growing on the fig surface. The treatment was more effective against Rhizopus spp. than against Alternaria spp. Contamination of fruit by Botrytis spp. and Penicillium spp. was also reduced by SO2. In conclusion, results showed that SO2 can be a potential tool to control postharvest rots and therefore increase the market life of fresh figs.  相似文献   

8.
Pre-storage application of 40% CO2 at 0 °C for 24 or 48 h and controlled atmosphere (12% O2 + 12% CO2) storage at 0 °C for up to eight weeks on decay control and quality of organic ‘Flame Seedless’ and ‘Crimson Seedless’ table grapes were studied as a postharvest disease control alternative. To simulate different potential field conditions, these organic treatments were applied to organic-grown grapes that were naturally infected (without inoculation), surface inoculated (berries inoculated by spraying with a conidia suspension), and nesting inoculated (clusters inoculated by placing in the middle an artificially infected berry) with the pathogen Botrytis cinerea, the cause of grape gray mold. Under these three conditions, a 40% CO2 for 48 h pre-storage treatment followed by controlled atmosphere reduced the gray mold incidence from 22% to 0.6% and from 100% to 7.4% after four and seven weeks, respectively. High CO2 pre-storage alone limited botrytis incidence in both naturally and artificially infected grapes, but was more effective when combined with CA. These treatments did not affect visual or sensory fruit quality. Exposure to high CO2 for 24 or 48 h effectively inhibited mycelial growth of B. cinerea in PDA plates incubated at 22 °C for up to 72 h. Conidia germination in PDA plates was reduced ∼60% after 12 h incubation. In vitro studies demonstrated a fungistatic effect, but further studies on the mechanism of action could improve treatment performance. This novel high CO2 initial fumigation followed by controlled atmosphere during storage or transportation could be a commercially feasible alternative for postharvest handling of organic and conventional table grapes. Our results encourage validating this combined physical treatment in other cultivars and under commercial conditions.  相似文献   

9.
This study evaluated the effects of sodium chlorite (SC) alone and its sequential treatment with edible coatings on browning inhibition and quality maintenance of fresh-cut d’Anjou pears. Edible coatings were prepared from chitosan (CH) and its water-soluble derivative carboxymethyl chitosan (CMCH), separately. Pear wedges were immersed in SC solution, followed by coating with CH or CMCH solutions. The samples were packed in unsealed bags and stored at 4 °C for subsequent color, firmness, and weight loss measurement. The effects of the SC and coating treatments on polyphenol oxidase (PPO) inhibition and microbial inactivation were also evaluated. Results indicated that SC exhibited significant (P < 0.05) inhibition of browning and PPO activity. The SC treatment was also strongly effective in inactivating Escherichia coli O157:H7 on pear slices. Coating SC-treated pear slices with CH adversely affected the quality of pear slices by accelerating the discoloration of cut surfaces and increasing the PPO activity. On the contrary, coating SC-treated samples with CMCH significantly prevented the browning reaction and inhibited PPO activity. In addition, SC and CH/CMCH coatings maintained tissue firmness and did not affect weight loss. Our study may provide a scientific basis for the use of SC + CMCH treatment as an alternative preservation treatment for fresh-cut fruits and vegetables.  相似文献   

10.
Controlled atmosphere (CA) treatments with ultralow oxygen (ULO) alone and in combinations with 50% carbon dioxide were studied to control grape mealybug, Pseudococcus maritimus (Ehrhorn) on harvested table grapes. Two ultralow oxygen levels, 30 and <0.01 μL L−1, were tested in both ULO and ULO + 50% CO2 treatments. The ULO treatments with the lower oxygen level were more effective than the ULO treatments at the higher oxygen level. The ULO + 50% CO2 treatments were more effective than the ULO treatments. Grape mealybug eggs were significantly more tolerant of ULO and ULO + CO2 treatments than nymphs and adults. A 14 day ULO treatment with 30 μL L−1 O2 at 2 °C did not achieve 100% mortalities of any life stage. In the presence of 50% CO2, the 14 d treatment achieved complete mortality of all life stages of the grape mealybug. A 3 d ULO treatment with <0.01 μL L−1 O2 at 2 °C resulted in 93.3% mortality of nymphs and adults. The 3 d ULO treatment in combination with 50% CO2 treatments, however, achieved complete control of grape mealybug nymphs and adults and caused 70.5% relative egg mortality. Complete egg mortality was achieved in a 10 d ULO + 50% CO2 treatment with <0.01 μL L−1 O2 at 2 °C. Both the 14 d CA treatment with 30 μL L−1 O2 and 50% CO2 and the 10 d CA treatment with <0.01 μL L−1 O2 and 50% CO2 were tested on table grapes and grape quality was evaluated after two weeks of post-treatment storage. The CA treatments did not have a significant negative impact on grape quality and were safe for table grapes. The study indicated that CA treatments have potential to be developed for postharvest control of grape mealybug on harvested table grapes.  相似文献   

11.
Rachis browning of table grapes after harvest is a significant problem, and water loss is considered the primary factor in browning. The major rachis desiccation and browning occurs during marketing at ambient temperatures and relative humidity (RH) which create high water vapor pressure deficits (WVPD). In this study the effect of WVPD and its components on rachis browning were examined on the two white seedless cultivars ‘Superior’ and ‘Thompson’. The grape clusters were stored at 20 °C or at 10 °C with low (70%) or high (>95%) RH, thus creating 4 WVPD levels. At each WVPD the clusters were held in open punnets, punnets sealed with low density polyethylene film or microperforated polyethylene, and examed every 2 or 3 d for weight loss, berry firmness, rachis dry weight and subjective rachis index. In addition, the rachis were photographed and image analysis employed to identify the level of browning. The results show that image analysis gave very similar patterns to subjective evaluation of rachis browning with correlation coefficients up to 0.90. However, image analysis detected an increase in browning before subjective evaluation. There was poor overall correlation between cluster weight loss and rachis dry weight to browning for ‘Superior’ grapes but a good correlation for ‘Thompson’. Rachis of ‘Superior’ suffered extensive browning at 20 °C even at high RH while rachis of ‘Thompson’ remained relatively green under similar conditions. ‘Thompson’ grape rachis remained green during the entire examination period (11 d) when held at high RH in either 10 °C or 20 °C. At high WVPD, microperforated packaging offered better control of browning in ‘Superior’ grapes than closed packaging, while clusters of ‘Thompson’ retained green rachis after 4 d in open punnets, and after 7 d in covered punnets. In summary, detailed analysis of rachis browning shows that water loss is an important but not the only factor in browning. Quantitative and objective measurement of rachis browning is likely to facilitate better communication of experimental data and higher resolution of processes which lead to browning.  相似文献   

12.
The control by ozone of postharvest decay of table grapes, caused by Botrytis cinerea and other pathogens, was evaluated in chambers and commercial storage facilities. Ozone at 0.100 μL/L or higher inhibited the spread of gray mold among stored grapes. Ozone diffusion into many types of commercial packaging was measured. Boxes made of uncoated paper corrugate inhibited diffusion more than those composed of coated paper corrugate, plastic corrugate, hard plastic, or expanded polystyrene. Internal packaging of hard plastic clamshell containers inhibited diffusion less than low density polyethylene cluster bags. Atmospheres of 0.100 μL/L ozone in the day and 0.300 μL/L at night reduced the natural incidence of gray mold by approximately 65% after 5–8 weeks of storage. Its effectiveness to control postharvest decay was compared to sulfur dioxide fumigation. After 68 days at 1 °C the incidence of gray mold among grapes stored in air, ozone, or with weekly sulfur dioxide fumigation was 38.8%, 2.1%, and 0.1%, respectively. However, decay by other fungi, such as Alternaria spp. and Penicillium spp., was controlled by sulfur dioxide, but not by ozone. In some tests, rachis appearance was moderately harmed by ozone. The combination of ozone use in storage following a single initial sulfur dioxide fumigation, or its use in between biweekly sulfur dioxide fumigations, controlled both gray mold and other pathogens and matched the commercial practice of initial and weekly sulfur dioxide fumigation. The use of both gases in this way reduced sulfur dioxide use greatly. Differences in flavor of grapes treated with ozone were not detectable compared to those stored in air, and grapes treated with ozone were preferred over those treated with sulfur dioxide.  相似文献   

13.
The potential of humidifying cold storage rooms to control moisture loss and quality of table grapes in different package designs was studied. Fruit were stored in cold rooms (−0.33 ± 0.32 °C or −0.12 ± 0.32 °C) with humidifier (95.0% RH) or no humidification (90.3% RH) respectively. Room humidification resulted in a 7.5% and 9.0% increase in RH inside the clamshell and open-top punnets multi-scale packages respectively in comparison to non-humidified storage, while there was no significant change in RH inside the 4.5 kg carry bag multi-packaging. The grapes were assessed for weight loss and SO2 injury at intervals during a 35 d period. After 21 d of cold storage under humidification, weight loss of grapes was significantly higher (P < 0.05) in packages with open-top punnets than clamshell punnets and carry-bags. After 35 days in non-humidified cold storage, grape weight losses were 1.45 ± 0.32%, 1.62 ± 0.21% and 2.01 ± 0.57% for the 4.5 kg carry-bag, 5 kg clamshell punnet and 5 kg open-top multi-packages, respectively. When fruit were stored inside the same types of multi-packages under humidification, the corresponding weight losses were 0.97 ± 0.34%, 1.08 ± 0.27% and 2.00 ± 0.57%. Cold storage humidification reduced the rate of stem dehydration and browning; however, it increased the incidence of SO2 injury in table grape bunches and caused wetting of the packages.  相似文献   

14.
‘Crimson Seedless’ is a popular table grape cultivar, but in warm-climates, its fruits often fail to develop adequate red color, even after they have been treated with ethephon. Application of abscisic acid (ABA) may improve color more effectively than ethephon, but its potential effects on postharvest quality must be considered before recommending its use on table grapes. Therefore, we compared the postharvest quality attributes of grapes treated preharvest with 250 μL L−1 ethephon, the current industry standard, to that of grapes treated with 150 or 300 μL L−1 ABA, or nontreated. Treatment with either ethephon or 150 μL L−1 ABA allowed grapes to be harvested 10 d before nontreated fruit, and fruits treated with 300 μL L−1 ABA attained marketable quality 30 d before nontreated fruit. Early harvest was possible because the treatments induced more rapid coloring of the grapes, and though total yield was not affected by any plant growth regulator (PGR), all PGRs doubled packable yields by improving the color of the grapes. ABA-treated grapes were characterized by superior appearance both in berries and clusters’ rachises compared to ethephon-treated and control grapes. Other quality attributes such as firmness, berry weight, decay incidence, and shatter remained unaffected among treatments. Therefore, ABA is an effective alternative to ethephon for enhancing the color and maintaining postharvest quality of ‘Crimson Seedless’ grapes.  相似文献   

15.
Fungal decay is a major cause of postharvest losses in strawberries. The traditional approach for controlling fungal decay is the use of fungicides. However, the use of fungicides has been questioned as a sustainable and safe method, and is also prohibited in many countries. One potential physical method for reducing fungal decay is application of a short-term hypobaric treatment prior to storage. In this study efficacy of postharvest hypobaric treatments to control natural rot development in strawberries was evaluated. Strawberries were treated with hypobaric pressures (25 kPaa, 50 kPaa and 75 kPaa) for 4 h at 20 °C and subsequently stored at 20 °C or 5 °C. A 50 kPaa treatment consistently delayed rot development in samples stored at either temperature confirming that the technique has potential as a non-chemical treatment. Moreover 50 kPaa treatments did not affect weight loss and firmness at either 20 °C or 5 °C. An initial increase in respiration rate was observed in 50 kPaa treated samples potentially indicating mild stress due to hypobaric treatment. An in vitro fungal study found that 50 kPaa treatment for 4 h did not affect the rate of radial growth of colonies of Botrytis cinerea and Rhizopus stolonifer, providing further evidence that the potential mechanism of hypobaric treatment is induction of the defence system within the fruit rather than a direct effect on fungal viability. Further molecular and biochemical research is required to evaluate the possible stimulation of resistance in fruit through short-term hypobaric treatments.  相似文献   

16.
The effectiveness of short hyperbaric treatments to control postharvest decay of sweet cherries (Prunus avium L., cv Ferrovia) and table grapes (Vitis vinifera L., cv Italia) was investigated. Sweet cherries and table grape berries were exposed to the pressure of 1140 mmHg (1.5 atm) for 4 and 24 h, respectively, in 64 L gas-proof tanks. Fruit kept at ambient pressure (near 760 mmHg, 1.0 atm) served as a control. Postharvest rots of sweet cherries arose from naturally occurring infections, whereas table grape berries were artificially wounded, exposed to the hyperbaric treatment, then the wounds inoculated with 20 μL of a Botrytis cinerea conidial suspension (5 × 104 spores mL−1). Sweet cherries were stored at 0 ± 1 °C for 14 d, followed by 7 d at 20 ± 1 °C. Table grapes berries were kept at 20 ± 1 °C for 3 d. On sweet cherries, hyperbaric treatment reduced the incidence of brown rot, grey mould, and blue mould, with respect to the control. Similarly, on treated table grapes a significant reduction of lesion diameter and percentage of B. cinerea infected berries was observed. Induced resistance was likely to be responsible for the observed decay reduction. To our knowledge, this is the first report on the effectiveness of short hyperbaric treatments in controlling postharvest decay of sweet cherries and table grapes.  相似文献   

17.
An integrated approach was evaluated that combined biological and chemical fumigation of table grapes to control postharvest gray mold caused by Botrytis cinerea. After fumigation of the grapes with ozone or sulfur dioxide during pre-cooling, the fruit were then exposed to continuous biofumigation by the volatile-producing fungus Muscodor albus during storage. Biofumigation was provided by in-package generators containing a live grain culture of the fungus. This grain formulation of M. albus survived the initial ozone or sulfur dioxide fumigation, but sulfur dioxide reduced its production of isobutyric acid, an indicator of the production of antifungal volatiles. Gray mold incidence was reduced among inoculated ‘Autumn Seedless’ grapes from 91.7 to 19.3% by 1 h fumigation with 5000 μL L?1 ozone, and further reduced to 10.0% when ozone fumigation and M. albus biofumigation were combined. The natural incidence of gray mold among organically grown ‘Thompson Seedless’ grapes after 1 month of storage at 0.5 °C was 31.0%. Ozone fumigation and M. albus biofumigation reduced the incidence of gray mold to 9.7 and 4.4, respectively, while the combined treatment reduced gray mold incidence to 3.4%. The use of commercial sulfur dioxide pads reduced the incidence to 1.1%. The combination of ozone and M. albus controlled decay significantly, but was less effective than the standard sulfur dioxide treatments. Although less effective than sulfur dioxide treatment, ozone and M. albus controlled decay significantly, and could be alternatives to sulfur dioxide, particularly for growers complying with organic production requirements.  相似文献   

18.
Summary A method to inoculate detached bean leaves with Botrytis cinerea or Sclerotinia sclerotiorum without using a carbon-nitrogen source is described. Leaves were incubated in plastic trays that were covered with transparent plastic bags to maintain air humidity above 95%. Spores of either fungus inoculated in water alone were generally unable to cause infection. Additions of KH2PO4 (62.5 mM) or of mixtures of inorganic phosphate and glucose (1.1 or 5.5 mM) to the inoculum stimulated fungal pathogenicity. Also spore concentration (2×105 or 2× 106 spores ml–1) influenced the ability to produce lesions. At 2× 106 spores ml–1 both fungi were able to infect primary bean leaves when inoculated in an inorganic phosphate solution. These conditions were used for a preliminary screening for resistance of 15 bean cultivars to both fungi. Only partial resistance to the pathogens was found. Since the procedure is simple and does not require blooming plants, it can be rapidly adopted in screening and breeding programs.Abbreviations Pi inorganic phosphate  相似文献   

19.
Postharvest diseases limit the storage period and marketing life of figs. The efficacy of chlorine dioxide by fogging was tested for the control of postharvest diseases of black fig (Ficus carica L. cv. Bursa Siyahi). Fruit were fogged with various concentrations of chlorine dioxide in a cold storage unit for 60 min at room temperature. Treated fruit were stored either in air or modified atmosphere bags for 7 d at 1 °C followed by 2 d shelf-life at 20 °C. Fogging at 300–1000 μL L−1 significantly reduced natural incidence of decay, most of which was gray mold. The efficacies of fogging at 500 and 1000 μL L−1 were at the same level and fogging at 1000 μL L−1 was superior to that at 300 μL L−1 in fruit stored in air. Modified atmosphere packaging did not improve the efficacy of fogging in reducing decay incidence. The epiphytic population on the fruit surface was similarly reduced by chlorine dioxide fogging. All treatments significantly reduced total microorganisms, fungal and bacterial populations in fruit. In addition, microorganisms in the storage atmosphere were significantly reduced. None of the treatments affected the visual quality and taste of fruit.  相似文献   

20.
Highbush blueberries (Vaccinum spp.) are a major export fruit crop of Chile which is stored at 0 °C and transported to markets in Asia, Europe, and the USA, using more than 15 d of maritime transportation. Under these conditions, gray mold caused by Botrytis cinerea can produce important economic losses. The effectiveness of sulfur dioxide (SO2) concentration × time treatments on gray mold control was determined in the laboratory and validated prior to refrigerating the fruit, using pallet scale SO2 fumigation treatment on the following blueberry cultivars: ‘Brigitta’, ‘Legacy’, ‘Liberty’ and ‘O’Neal’. In inoculated ‘Brigitta’ and ‘Liberty’ blueberries, gray mold prevalence varied from 97.2% to 97.5% in non-treated fruit, and this value was reduced from 7.9% to 6.1% in blueberries that were exposed to a SO2 concentration × time (Ct) product of 400 (μL L−1) h. The relationship between SO2 Ct products and gray mold prevalence under laboratory conditions was best explained by exponential models, which had a determination coefficient (R2) that ranged from 0.88 to 0.96. The estimated EC90 values varied between 245 and 400 (μL L−1) h, and the SO2 Ct between 250 and 350 (μL L−1) h was validated using a pallet scale application treatment to obtain the best control and minimal variation. No visual phytotoxicity symptoms of SO2 were observed with the Ct that was tested in this study. Therefore, SO2 fumigation was demonstrated to be an effective and practical technology for reducing the risk of blueberry gray mold decay during storage, and further effort should be given to register the use of this product for blueberries in the main Chilean export markets.  相似文献   

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