共查询到18条相似文献，搜索用时 671 毫秒
1.
本文建立了采用高效液相色谱法同时检测火麻籽油中10种酚类物质的方法,10种单体酚检出限为0.05～0.12μg/mL,线性相关系数为0.9970～0.9998,回收率为85%～108%。检测了8份不同产地火麻籽油的总酚、总黄酮含量及体外抗氧化活性指标（DPPH、FRAP、ABTS+·）。总酚含量为1.20～17.35mg对香豆酸/100g,总黄酮含量为7.61～51.72mg柚皮苷/100g。DPPH清除能力为9.03～19.71mmol TE/100g,FRAP还原能力为13.12～30.09mmol TE/100g,ABTS+•清除能力为29.40～358.37mmol TE/100g。相关性分析表明,总酚含量与DPPH、ABTS+•清除能力和FRAP还原能力呈显著正相关;总黄酮含量与DPPH清除能力、FRAP的还原能力呈显著正相关。 相似文献
2.
《热带作物学报》2017,(12)
以新鲜桑葚汁为原料,分析高压均质处理对桑葚汁中抗氧化成分(总酚、总黄酮、花色苷)及其抗氧化活性的影响。结果表明,随着高压均质处理压力的增加,与桑葚原汁相比,其总酚、总黄酮和花色苷含量均降低,且经过160 MPa均质处理后降低得最为显著(p0.05),分别减少了39.91%、27.35%、24.41%。经过不同压力均质处理后的桑葚汁的ABTS、DPPH自由基清除能力和FRAP抗氧化活性与桑葚原汁相比均下降,且随着处理压力的增加,桑葚汁的ABTS、DPPH自由基清除能力和FRAP抗氧化活性逐渐降低,说明高压均质处理对桑葚汁中抗氧化成分造成了一定降解,进而影响其抗氧化能力。 相似文献
3.
桃金娘果实提取物抗氧化活性研究 总被引：1，自引：0，他引：1
以去除种子后的桃金娘果实为试验材料,分别采用等体积的水与体积分数80%甲醇进行提取,测定2种提取物的总酚与总黄酮含量,并对2种提取物的DPPH自由基与ABTS自由基清除能力以及总抗氧化能力进行评价。结果表明,桃金娘果实的2种提取物均具有不同程度的多酚含量与抗氧化活性,体积分数80%甲醇提取物的总酚与总黄酮含量、DPPH与ABTS自由基清除能力以及总抗氧化能力均显著高于水提取物。研究结果表明,桃金娘果实多酚物质具有良好的抗氧化活性。 相似文献
4.
采用DPPH体系、ABTS体系和Oyaizu法对白沙绿茶的甲醇提取物、70%乙醇提取物和水提取物的抗氧化活性进行测定,并利用HPLC-MS对活性较高的乙醇提取物的抗氧化特征性成分进行分析。结果表明:白沙绿茶3种提取物均具有一定的抗氧化能力,呈浓度效应关系。其中3种提取物中以70%乙醇提取物对DPPH和ABTS清除能力最强,而还原力则弱于水提取物和甲醇提取物。通过HPLC-MS从70%乙醇提取物中得到24个酚类物质,其中15个酚类物质被鉴定为新绿原酸、花旗松素-3-O-芸香糖苷、绿原酸、隐绿原酸、去甲氧基姜黄素、表儿茶素、山奈酚-3-O-芸香糖苷、花旗松素-3-O-葡萄糖苷、槲皮素-双己糖苷、Di-O-咖啡酰奎宁酸、槲皮素、山奈素-3-O-戊糖苷、牛蒡子苷、杨梅素和山奈素。 相似文献
5.
目的对辽东丁香枝的总提液、氯仿层、乙酸乙酯层、正丁醇层、水层进行抗氧化活性的评定。方法采用清除DPPH自由基、清除ABTS+.自由基、还原性和总酚含量测定的方法,利用分光光度法测定辽东丁香枝不同部位在不同浓度下的抗氧化能力及总酚含量。结果辽东丁香枝不同部位具有明显的清除DPPH、ABTS+.自由基的能力,且有较好的还原能力和较高的总酚含量。在清除DPPH自由基体系中,乙酸乙酯层的抗氧化能力最强,其IC50值最低为0.033mg/m L,正丁醇层和氯仿层的次之,IC50分别为0.0546,0.0638mg/m L,在清除ABTS+自由基体系中,各部位抗氧化活性大小为:乙酸乙酯层总提液氯仿层正丁醇层水层;还原性体系测定中,乙酸乙酯层的还原能力接近于BHT的;乙酸乙酯层的总酚含量最大,为164.7μg/mg,接近BHT的168.2μg/mg。结论辽东丁香枝具有较好的抗氧化活性,其抗氧化能力与总酚含量有较好的相关性,需要进一步深入研究,开发为天然抗氧化剂。 相似文献
6.
不同品种桑葚叶总酚含量及其抗氧化活性比较 总被引：1，自引：0，他引：1
为了研究不同品种桑葚叶的总酚含量差异及应用价值,以收集引种的40个品种桑葚叶为研究对象,采用超声波辅助提取桑葚叶中酚类物质,以总酚提取率为指标,利用单因素试验和正交试验考察各因素对超声辅助提取桑葚叶总酚提取率的影响;采用DPPH自由基清除能力评价不同品种桑葚叶提取物的抗氧化能力,同时对不同品种桑葚叶中总酚含量及其抗氧化能力进行相关性分析。结果表明：超声辅助提取桑葚叶总酚的最佳工艺条件为：超声温度65 ℃、超声时间30 min、固液比1∶45（g/mL）、乙醇浓度60%,4个因素对桑葚叶总酚提取率影响大小顺序为：超声温度>超声时间>固液比>乙醇浓度。不同品种桑葚叶总酚含量差异较大,其中‘条桑五号’总酚含量最高,为（26.35 ± 0.29）mg/g,‘滇桑’总酚含量最低,为（20.44 ± 0.15）mg/g;不同品种桑葚叶抗氧化活性也存在差异,且趋势与总酚含量基本一致,‘条桑五号’桑葚叶抗氧化活性最强,清除DPPH自由基能力IC50为（77.64 ± 0.34）mg/L,总抗氧化能力（FRAP）TEAC值为（2.58 ± 0.11）mmol/g;‘滇桑’桑葚叶抗氧化活性最弱,清除DPPH自由基能力IC50为（210.30 ± 0.19）mg/L,总抗氧化能力（FRAP）TEAC值为（0.73 ± 0.04）mmol/g。桑葚叶总酚含量与其提取物抗氧化能力呈正相关,选择总酚含量高的桑葚品种栽培,可提高桑葚的综合附加值。 相似文献
7.
《中国糖料》2021,(4)
为了探究和分析红甜菜的抗氧化活性物质,本研究以红甜菜块根为试验材料,通过酶标仪-微孔板建立了3种微量FRAP、DPPH和ABTS体系,来评价红甜菜水提取物的体外抗氧化活性。结果表明:红甜菜水提取物具有较好的总抗氧化能力,对DPPH自由基和ABTS阳离子自由基有较强的清除能力,最高清除率分别达91.92%和83.79%。通过国标法提取并测定红甜菜块根中甜菜红素含量,约为2.53%。对甜菜红素含量与以上3个抗氧化指标进行相关性分析发现,它们之间呈显著正相关,相关系数为0.965～0.995,证明甜菜红素是红甜菜中提取物的主要活性成分。研究结果可为今后深入研究红甜菜提取物的体外抗氧化活性以及深度开发和利用红甜菜提供参考。 相似文献
8.
椰子种皮油提取物的抗氧化活性研究 总被引：1，自引：0，他引：1
测定椰子种皮油提取物中的总酚含量,对羟基自由基、DPPH（1,1-二苯基-2-三硝基苯肼）自由基、ABTS（2,2连氮-双-（3-乙基苯并噻咪唑-6-磺酸）自由基清除能力和总抗氧化能力等抗氧化活性指标。结果表明,椰子种皮油提取物中含有较高的总酚含量（68 mg/g）,提取物浓度为0.1 mg/mL时对羟基自由基的清除率为56.89％,对DPPH自由基的清除率为71.0％,对ABTS自由基的清除率为96.4％,表明椰子种皮油提取物具有很好的抗氧化活性。 相似文献
9.
《中国油料作物学报》2017,(2)
油菜籽富含多酚。菜籽多酚分为酚酸和缩合单宁,其中酚酸包括游离酚酸、酯化酚酸和结合不可溶酚酸,游离酚酸约占总酚的9%~16%,芥子酸是最主要的游离酚酸,占总游离酚酸的70%以上;芥子碱是最主要的酯化酚酸,占总酚的80%以上。多酚的萃取普遍采用有机溶剂萃取法,其中,70%甲醇是最常用的萃取溶剂之一,采用超声波辅助可缩短萃取时间,提高萃取效率;福林酚法、香草醛法和高效液相色谱法是目前国际上普遍采用的总酚、单宁和酚酸测定方法,其中常采用质谱技术对未知多酚成分进行定性。菜籽多酚具有良好的抗氧化活性,一般采用二苯基苦基肼(DPPH)法、铁离子还原抗氧化能力(FRAP)法、氧自由基吸收能力(ORAC)法等方法进行抗氧化活性评价;多酚类化合物的抗氧化活性与羟基和甲氧基的数量及在芳香环上的位置密切相关,羟基肉桂酸衍生物的抗氧化活性优于苯甲酸衍生物。在制油前对油菜籽进行热处理可增加籽和油中多酚含量,并使芥子酸脱羧转化为抗氧化活性更高的canolol,canolol具有脂溶性,压榨制油后,56%~83%的canolol从油菜籽中转移至油中,从而显著改善油的氧化稳定性;脱酸、脱色和脱臭等精炼导致油中多酚和canolol损失严重。未来应进一步采用液相色谱-质谱和核磁共振技术鉴定未知多酚成分的结构并进行定量分析,采用基于细胞模型的生物评价法,系统深入开展菜籽多酚的抗氧化活性研究,为多酚的开发利用提供理论支撑;同时应加强油脂适度精炼技术的研发与推广,尽可能减少精炼过程中多酚的损失,提高油脂营养价值。 相似文献
10.
为比较不同品种荔枝果皮酚类物质含量、组成及其抗氧化活性差异,用80%甲醇分别提取6个品种荔枝果皮中的酚类物质,测定其总酚和总黄酮含量。采用铁离子还原能力（FRAP）和自由基离子清除能力（ABTS）等方法评价其抗氧化活性。通过高效液相色谱（HPLC）鉴定不同的单体酚并分析其在不同品种荔枝果皮之间的差异。结果表明,不同品种荔枝果皮的酚类物质含量为28.69~68.48 mg/g,不同品种荔枝果皮的酚类物质含量和抗氧化能力存在显著的差异（P<0.05）。酚类物质的含量、FRAP和ABTS抗氧化活性能力均以‘荔枝王’为最高,分别为68.48 mg/g、546.31 μmol/g和511.25 μmol/g。液相色谱结果鉴定出‘糯米糍’中的8种单体酚,而含量相对较高的是A型原花青素三聚体和原花青素A2。研究结果可为荔枝加工废弃物荔枝果皮的开发利用提供理论依据。 相似文献
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12.
Maria F. Quintero-Soto Ana G. Saracho-Peña Jeanett Chavez-Ontiveros Jose A. Garzon-Tiznado Karen V. Pineda-Hidalgo Francisco Delgado-Vargas Jose A. Lopez-Valenzuela 《Plant foods for human nutrition (Dordrecht, Netherlands)》2018,73(2):122-129
Chickpea (Cicer arietinum L.) genotypes, nine kabuli from Mexico and 9 desi from other countries, were investigated for their phenolic profiles and antioxidant activity (AA). Phenolics in methanol extracts (ME) were analyzed by ultra-performance liquid chromatography coupled to diode array detection and mass spectrometry (UPLC-DAD-MS), whereas the AA was measured as Trolox equivalents (TE) by ABTS, DPPH and FRAP methods. Twenty phenolic compounds were identified in the ME and their levels showed a great variability among the chickpea genotypes. Phenolic acids and flavonoids were the most abundant compounds in kabuli and desi genotypes, respectively. The AA values (μmol TE/ 100 g dw) by ABTS (278–2417), DPPH (52–1650), and FRAP (41–1181) were mainly associated with the content of sinapic acid hexoside, gallic acid, myricetin, quercetin, catechin, and isorhamnetin, suggesting they are the main compounds responsible for the AA. The sum of the AA obtained for standards of these compounds evaluated at the concentration found in the extracts accounted for 34.3, 69.8, and 47.0% of the AA in the extract by ABTS, DPPH, and FRAP, respectively. In the AA by DPPH, most of the mixtures of these compounds resulted in synergistic interactions. Three desi genotypes with black seeds (ICC 4418, ICC 6306, and ICC 3761) showed the highest AA and flavonoids content, whereas the most promising kabuli genotypes were Surutato 77, Bco. Sin. 92, and Blanoro that showed the highest values of phenolic acids. These genotypes represent good sources of antioxidants for the improvement of nutraceutical properties in chickpea. 相似文献
13.
Bakasso S Lamien-Meda A Lamien CE Kiendrebeogo M Millogo J Ouedraogo AG Nacoulma OG 《Pakistan journal of biological sciences: PJBS》2008,11(11):1429-1435
Aqueous acetone extracts prepared from five Indigofera species of Burkina Faso, namely Indigofera colutea (Burm.) Murril., I. macrocalyx Guilld et Perr., I. nigritana Hook f., I. pulchra willd. and I. tinctoria L., were investigated for their phytochemical composition and their antioxidant activities. Standard methods and TLC were used to screen the phytochemical composition. The total phenolic and flavonoid content of extracts were assessed by Folin-Ciocalteu and AlCl3 methods, respectively. These extracts were also evaluated for their antioxidant potentials using ferric reducing antioxidant power (FRAP), 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulphonate) (ABTS) assays. Flavonoids, saponins, quinones, sterols/triterpenes and tannins were present in all these species except for I. pulchra where quinones were not found. Gallic acid, caffeic acid, rutin and myricetin in I. colutea; gallic acid, quercitrin, myricetin in I. tinctoria; galangin and myricetin in I. macrocalyx were identified by thin layer chromatography. Among these, I. colutea, I. tinctoria, I. nigritana and I. macrocalyx, which had the highest phenolic content, were also found to possess the best antioxidant activities. The results indicated a good correlation between antioxidant activities and total phenolic content (p<0.05 for FRAP/DPPH and DPPH/ABTS and p<0.01 for FRAP/ABTS). These plants represent promising sources of natural antioxidants and these findings give scientific bases to their ethnopharmacological uses. 相似文献
14.
Zoran Maksimovi&#x; 《Industrial Crops and Products》2008,28(3):356-360
The antioxidant activity in 70% aqueous acetone extracts of the herb of Ambrosia artemisiifolia L., Asteraceae was evaluated with regards to total polyphenol and flavonoid contents. Antioxidant activity has been assessed by two commonly used in vitro tests, based on determination of ferric-reducing antioxidant power (FRAP assay) and DPPH free radical scavenging ability (DPPH assay), against butylated hydroxytoluene (BHT), ascorbic acid and quercetin, which were used as positive control substances. The results of both antioxidant tests showed that the plant material expressed a considerable activity (DPPH IC50 = 27.6 μg/ml; FRAP value = 2.37 mmol Fe2+/g), attributed to both flavonoid aglyca and resembling glycosides, as verified by dot-blot TLC analysis. 相似文献
15.
Jayawardena Nilakshi Watawana Mindani I. Waisundara Viduranga Y. 《Plant foods for human nutrition (Dordrecht, Netherlands)》2015,70(1):71-76
The total phenolics contents, total antioxidant capacity (TAC) and starch hydrolase inhibitory activity of the aqueous extracts of 10 edible plants and the stability of these parameters after the gastric and duodenal digestion in an in vitro model was investigated. The TAC was evaluated using the oxygen radical absorbance capacity (ORAC) assay, ferric reducing antioxidant power (FRAP) and 2, 2-diphenyl-1-picrylhydrazyl (DPPH?) and 2, 2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS?+) radical scavenging assays. Characterization and quantification of five polyphenol compounds which were previously identified to be present in all the selected plants were carried out. None of the extracts showed a decrease in the total phenolics content or the ORAC and FRAP values following digestion. None of the quantified phenolic compounds had decreased during any of the digestion phases – an observation which was deemed as beneficial in terms of therapeutic properties. Overall, the parameters analyzed were relatively stable throughout the digestive process in all the extracts.
相似文献16.
Agnieszka Viapiana Marek Wesolowski 《Plant foods for human nutrition (Dordrecht, Netherlands)》2017,72(1):82-87
The aim of this work was to evaluate the antioxidant potential of teas prepared from twenty-four commercially available berries and flowers of Sambucus nigra L. in relation to their phenolic profile, as reflected by the most representative phenolic acids (caffeic, chlorogenic, p-coumaric, ferulic, gallic and syringic acids); flavonols (quercetin, kaempferol, myricetin and rutin); and total phenolic (TPC), phenolic acid (TAC) and flavonoid (TFC) contents. The infusions prepared from elderflowers contained more abundant phenolic compounds than the elderberry infusions. The TPC of these infusions ranged from 19.81 to 23.90 mg of gallic acid equivalents/g dry weight of sample (GAE/g DW) for elderberries and from 15.23 to 35.57 mg GAE/g DW for elderflowers, whereas the TFC ranged from 2.60 to 4.49 mg of rutin equivalents/g dry weight of sample (RUTE/g DW) in elderberry infusions and from 5.27 to 13.19 mg RUTE/g DW in elderflower infusions. Among the phenolic compounds quantified in this study, quercetin (2.07–9.48 mg/g DW) and myricetin (1.17–9.62 mg/g DW) had the highest concentrations in the teas prepared from berries and flowers, respectively. Moreover, the antioxidant potential of elder infusions assessed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical and ferric reducing antioxidant power (FRAP) assays revealed that the teas prepared from flowers had higher mean DPPH and FRAP activities than the teas prepared from berries. Therefore, elder beverages could be important dietary sources of natural antioxidants that contribute to the prevention of diseases caused by oxidative stress. 相似文献
17.
CM Donado-Pestana JM Salgado A de Oliveira Rios PR Dos Santos A Jablonski 《Plant foods for human nutrition (Dordrecht, Netherlands)》2012,67(3):262-270
Intervention strategies regarding the biofortification of orange-fleshed sweet potato, which is a rich source of carotenoids for combating vitamin A deficiency, are being developed in Brazil. This study was conducted to evaluate the concentrations of individual carotenoids, total phenolic compounds and antioxidant capacity in the roots of four biofortified sweet potato cultivars that were raw or processed by four common heat treatments. HPLC, Folin-Ciocalteu, DPPH and ABTS assays were used. All cultivars showed high levels of carotenoids in raw roots, predominantly all-trans-β-carotene (79.1-128.5?mg.100?g(-1) DW), suggesting a high estimated vitamin A activity. The CNPH 1194 cultivar reported carotenoids values highest than those of other cultivars (p?相似文献
18.
To investigate the relationship between reducing sugars and phenolic retention of brown rice after enzymatic extrusion, reducing sugars, total phenolic content, total antioxidant activity, and individual phenolic acids of brown rice extruded with different α-amylase concentrations (0.1%, 0.5%, 1%, w/w) were evaluated. Reducing sugars were produced during enzymatic extrusion and significantly increased with the increasing enzyme concentration. Compared with traditional extrusion, the enzymatic extrusion with 1% amylase significantly increased the phenolic retention, DPPH value, FRAP value, and ABTS value by 22.4%, 19.5%, 14.7%, and 41.5%, respectively. The retention of most free phenolic acids was also increased with the increasing enzyme concentration. Besides, the correlation analysis indicated that the content of reducing sugars was positively correlated with total phenolic content (r = 0.915), DPPH value (r = 0.882), FRAP value (r = 0.861), ABTS value (r = 0.867) and free individual phenolic acids (r = 0.595–0.943) of treated brown rice. These results suggested that reducing sugars might protect phenolic compounds during enzymatic extrusion. 相似文献