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棉花黄萎病抗性的分子研究进展 总被引：8，自引：3，他引：8
本文综述了棉花黄萎病菌的分子鉴定，棉花黄萎病抗性的遗传基础，分子标记在棉花抗黄萎病方面的应用，棉花黄萎病抗性基因的克隆及转化等方面的研究进展。 相似文献
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棉花主要病害研究概要 总被引：16，自引：2，他引：16
以棉花枯、黄萎病为主,就我国近50年来,在棉花病害研究方面取得的进展做了较详细的总结."全国棉花枯、黄萎病协作组"的成立,极大地促进了棉花病害研究工作的进展,突出表现在棉花枯、黄萎病菌生理型(小种)的鉴定,棉花的抗病机理和抗病鉴定方法研究,棉花种质资源材料和新品种系的抗病性鉴定,落叶型黄萎病的研究,抗病育种及种衣剂的研制等方面.并认为应加强棉花抗枯、黄萎病遗传,黄萎病菌致病力分化,以及抗虫棉病害发生特点、机理及抗病育种等方面的研究工作. 相似文献
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有关棉花黄萎病（Verticillium dahliae Kleb．）抗性遗传研究的几个问题 总被引：3，自引：0，他引：3
棉花黄萎病抗性遗传问题迄今尚未获得肯定的结论。据作者等估计，这可能和寄主（棉株）和寄生物（黄萎病镰刀菌）之间的关系、黄萎病菌不同菌系间的互作以及影响抗性鉴定的正确性等问题有关。为此，必须创设有利条件，使寄主和病原菌间的关系得以充分表现；必须了解病原菌菌系间的互作，寄主必须有较大的群体。这一切都是为了寄主和病原物问的关系得以充分体现，为抗性遗传研究奠定基础。抗性遗传研究宜用单菌系接种，并且宜按数量性状和质量性状遗传方式同时进行研究和分析，以便得到比较确切可靠的结果。 相似文献
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棉花黄萎病菌与抗黄萎病遗传育种研究进展 总被引：21，自引：1，他引：21
简要综述了棉花黄萎病菌及抗黄萎病遗传育种的研究进展。研究表明 ,各地的棉花黄萎病菌均存在致病力的分化 ,其致病机理是病菌侵入棉花后菌丝及孢子在导管内大量繁殖 ,同时刺激邻近的薄壁细胞产生胶状物质及侵填体而堵塞导管 ,使水分和养分运输发生困难 ,更重要的是病菌在棉株体内产生的糖蛋白毒素作用的结果。棉花抗黄萎病的遗传方式争论较大 ,但一般在温室由单一菌系接种鉴定时棉花黄萎病抗性表现为单基因遗传 ,而在田间病圃或用多菌系混合鉴定时 ,棉花黄萎病抗性表现为多基因遗传。由于陆地棉内缺乏高抗黄萎病资源 ,给棉花抗黄萎病育种带来一定困难 ,但 90年代以来 ,已育成 86 - 6、川 73 7、川 2 80 2、豫棉 1 9号、豫棉 2 1号等一些抗黄萎病的新品种。上述抗黄萎病品种在棉花黄萎病综合防治中起了重要作用 相似文献
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为了研究黄萎病菌的侵染机制,通过农杆菌介导的转化方法,将绿色荧光蛋白基因sGFP导入落叶型黄萎病菌VD07038,将红色荧光蛋白基因mCherryRFP导入非落叶型黄萎病菌Bp2中,分别获得了具有绿色、红色荧光信号的阳性转化子。经过分子验证和连续继代培养,证明了这些转化子具有遗传稳定的对潮霉素的抗性。通过对转化子的菌落形态、生长速度和致病力进行检测,发现大部分转化子与野生型基本一致,少量转化子发生变异,其中转化子Bp2R-30不能产生微菌核,致病力显著下降。利用荧光显微镜观察了转化子VD07038G-10在感病棉花品种苏棉22幼苗根部的侵染情况。结果表明,在接菌12 h后VD07038G-10的孢子可吸附在根表面;接种7~9 d后,菌丝入侵到棉花根部的维管组织。本研究获得的荧光蛋白标记的棉花黄萎病菌VD07038G-10可用于实时观测黄萎病菌侵染棉花根系的过程,并且可以定量鉴定不同棉花品种对该菌系的抗性,为棉花黄萎病菌抗性鉴定提供一种新方法。 相似文献
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Lu Ningning Zhao Yunlei Wang Hongmei Chen Wei Zhao Pei Gong Haiyan Cui Yanli Sang Xiaohui Zhang Kai 《棉花学报》2019,31(3):254-262
[Objective] The objective of this study was to identify the stable quantitative trait loci (QTLs) related to Verticillium wilt resistance in cotton. [Method] In this study, a population of 111 recombinant inbred lines (RILs) strains was developed by crossing a highly resistant parental line "Changkangmian" to Verticillium wilt and the susceptible parent TM-1. The complete composite interval mapping method was adopted to detect QTLs by Verticillium wilt disease index in multiple environmental conditions and periods in Anyang and Verticillium wilt affected areas of Xinjiang. Simple sequence repeat (SSR) markers of polymorphism were screened for genetic mapping. [Result] The genetic map was constructed by 40 simple sequence repeat (SSR) markers, consisted of 12 linkage groups with total length of 212.5 centimorgan (cM). A total of six QTLs related to the resistance to Verticillium wilt were obtained. The likelihood of odd (LOD) values ranged from 2.51 to 5.55. The maximum phenotypic variation explained (PVE) 20.34%, and the minimum PVE 6.93% were recorded. Among detected QTLs, qVR-D05-1 was detected in both Verticillium wilt affected fields in Anyang and Xinjiang in July 2015 and July 2016 with PVE of 12.96% and 20.34%, respectively. [Conclusion] This study can provide a potential reference for mapping stable QTLs related to resistance to Verticillium wilt. 相似文献
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木霉菌对棉花黄萎病菌拮抗的作用 总被引：2，自引：0，他引：2
以绿色木霉(Trichoderma viride)、康氏木霉(Trichoderma koningii)，以及二株未知种名的木霉菌株(Trichoderma spp.)为供试菌，采用对峙培养测定其对棉花黄萎病菌(Verticillium dahliae)的抑制作用，并研究了四种木霉菌产生的非挥发性代谢物对菌丝干重的影响。结果表明：在对峙培养中，木霉菌对棉花黄萎病菌均有明显抑制作用，木霉菌产生的非挥发性代谢物可以强烈抑制棉花黄萎病菌生长，明显降低其菌丝干重，并具有热稳定性。光学显微镜下观察，木霉菌丝在棉花黄萎病菌丝上平行或波浪式生长且棉花黄萎病菌丝出现细胞原生质浓缩和菌丝断裂等现象。 相似文献
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Isolation and Characterization of Endophytic Bacillus methylotrophicus LH-L3 Antagonist of Cotton Verticillium Wilt 总被引：2，自引：2，他引：0
The aim of this study is to obtain endophytic Bacillus with control effect on cotton Verticillium wilt. [Method] The cotton tissue grinding liquid was heated at 80 ℃, and spread onto the Luria-Bertani ager medium. The strains with better inhibitory effects on Verticillium dahliae Vd084, a strain with strong pathogenicity, was identified by the morphology, physiological and biochemical traits as well as 16S rRNA, gyrB and rpoB sequences, and its control effect on cotton Verticillium wilt was determined by pot experiments. [Result] 61 similar Bacillus strains were isolated from cotton plants. Among them, 17 strains could inhibit Verticillium dahliae Vd084. A strain named LH-L3 with a relatively higher antagonistic activity and a broad inhibition spectrum was obtained in the second screening. LH-L3 was classified into Bacillus methylotrophicus. Compared with the control, the emergence rate, plant height, root length, fresh weight of aerial part and underground part of cotton with 106 mL－1 LH-L3 suspension drenching for 3 times increased by 42.85%, 10.24%, 23.83%, 10.05% and 97.62%, respectively. And control effect on Verticillium wilt was 85.24%. [Conclusion] LH-L3 had good growth promotion and Verticillium wilt control effects and showed good potential for biological control. 相似文献