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1.
猪附红细胞体PCR诊断方法的建立 总被引：1，自引：0，他引：1
据猪附红细胞体重庆株16S rRNA基因的序列特点设计合成种特异性引物,建立了猪附红细胞体PCR诊断方法.该方法能特异性扩增840 bp的猪附红细胞体16S rRNA基因片段,而对牛温氏附红细胞体、羊附红细胞体、猫血巴尔通氏体CA株、绵羊肺炎支原体、猪肺炎支原体、假单孢茵、大肠杆茵、沙门氏茵、肝片吸虫、葡萄球菌等的基因组DNA没有扩增条带出现.对猪附红细胞体基因组DNA的最小检测量为0.16 fg.通过对30份临床样品的检测,21份猪附红细胞体感染为阳性,其余为阴性.结果表明:建立的PCR诊断方法具有很高的敏感性和特异性,可用于猪附红细胞体的临床诊断和流行病学调查. 相似文献
2.
[目的]弄清一株血液征状与鼠巴尔通氏体病相似的小鼠血源细菌的分类学地位及其与鼠巴尔通氏体的区别。[方法]通过细菌常规鉴定及16SrRNA基因系统发育进化分析,对其进行了分类鉴定。利用免疫荧光和透射电镜观察、人工感染及免疫保护等试验,对该茵生物学特性进行研究。[结果]常规分类鉴定显示分离到的细菌与丹毒科丹毒属细菌靠近,但其培养、染色及生化特性与该属的猪丹毒杆菌有较大差异。16SrRNA基因序列与猪红斑丹毒丝菌相似性达98．4％,系统进化树分析该菌与猪红斑丹毒丝菌亲源关系最近。免疫荧光和透射电镜观察结果表明该茵寄生在红细胞内部,但红细胞表面分泌有该细菌抗原成分。该细菌可通过尾静脉注射、腹腔注射、口腔感染及接触感染致死小白鼠。[结论]建议将该细菌归为BXⅢ门（Firmicutes）Ⅱ纲（Mollicutes）V目（未定）I科（Erysipelotrichace—ae）I属（Erysipelothrix）的一新种（Erysipelothrixmuris sp．nou）。 相似文献
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住家附近诱捕到14只家鼠,经皮下注射醋酸可的松8周,建立卡氏肺孢子虫肺炎,取家鼠肺组织制成接种物。1～8号实验猫于接种前皮下注射醋酸可的松8d,然后肺内接种1.3×105卡氏肺孢子虫包囊;9～16号实验猫则直接肺内接种2.6×105卡氏肺孢子虫包囊。接种后,注射醋酸可的松的1～8号实验猫出现严重的临床症状;病理变化呈典型的卡氏肺孢子虫肺炎。9～16号实验猫为亚临床症状;肺脏呈轻微肿胀。实验结果表明实验猫免疫力低下时,接种外源性卡氏肺孢子虫包囊可导致典型的卡氏肺孢子虫肺炎;健康猫接种外源性卡氏肺孢子虫包囊则为隐性感染。并对肺脏等组织内的虫体进行详细的形态观察。 相似文献
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为了解温州地区犬猫肠道寄生虫的感染情况,采用直接涂片法、饱和盐水漂浮法、沉淀法对采自不同饲养场所的267份新鲜粪便进行检查。结果显示,有74份阳性样品,共检出7种寄生虫虫卵,以弓首蛔虫虫卵和钩虫虫卵为主。流浪犬猫、未驱虫犬猫的感染率明显高于宠物犬猫和驱虫犬猫,有弓首蛔虫、钩虫、复孔绦虫、曼氏迭宫绦虫、贾第虫5种人畜共患寄生虫被检出。流浪犬猫感染的寄生虫种类多,感染率高,且携带多种人兽共患寄生虫。研究表明,温州地区犬猫肠道寄生虫感染比较普遍且种类复杂,这为进一步了解温州地区犬猫寄生虫感染状况和人畜共患寄生虫病防控提供基础材料。 相似文献
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血巴尔通体病是以血巴尔通体寄生于猫或犬血液中红细胞表面损伤红细胞,引起机体严重贫血、甚至死亡为特征的疾病。人感染血巴尔通体一般称为"猫抓病",猫血巴尔通体病也有人称之为"猫附红细胞体病,是引起猫传染性贫血原因之一。为此对此病的流行病学、发病机理临床症状等几方面做出分析,仅供参考。 相似文献
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通过临床检查、血常规检查、生化检查和X射线检查,确诊了一例猫右后肢股骨颈骨折.采用股骨头切除术进行治疗,术后对其进行康复训练,患猫逐渐恢复了正常运动能力.股骨头切除术对于猫股骨颈骨折病具有良好的治疗效果. 相似文献
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《四川农业大学学报》2019,(5)
【目的】建立猫慢性肾衰(CRF)模型,为研究猫CRF的发病机制及评价猫CRF药物的疗效奠定基础。【方法】将三聚氰胺与三聚氰酸1∶1混合物按体重给予20、10、5 mg/kg剂量拌料连续饲喂试验组猫21 d(1次/d),后再按1次/2 d饲喂14 d(7次),于0、22、36 d采集血液、尿液检测生理生化指标;按照国际公认的猫慢性肾衰分级标准,判定模型建立是否成功;死亡猫剖检取肾组织制作病理切片,HE染色观察其病理学变化。【结果】①试验组猫出现多饮多尿、消瘦等肾衰临床症状,血肌酐、尿素氮升高,红细胞数目、尿比重下降,并有尿蛋白产生。②肾脏病理组织学结果显示,肾小管上皮细胞空泡变性、坏死,肾小管上皮细胞脱落在管腔中央形成肾小管上皮细胞管型,肾小球结构不完整。【结论】采用低(5 mg/kg)、中(10 mg/kg)剂量三聚氰胺与三聚氰酸1∶1混合物先每天1次连续饲喂猫21 d、再按两天1次饲喂14 d的方法能建立稳定的轻度或中度猫CRF模型。 相似文献
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猫传染性腹膜炎是由猫冠状病毒引起的渐进性、高致死性疾病。患猫可能出现食欲减退、精神沉郁、体重下降、发热、呼吸困难等症状。本文对两例幼龄公猫进行临床、血液学、影像学检查，均出现不同程度的胸腹腔积液，并伴有贫血、总胆红素升高、白蛋白与球蛋白比值降低等情况，结合猫冠状病毒核酸检测结果，病例1确诊为湿性猫传染性腹膜炎，病例2确诊为混合性猫传染性腹膜炎。对两病例使用核苷类似物GS-441524制剂并配合保肝药物，进行数月跟踪治疗，各项指标恢复正常，治疗效果显著。本文可为猫传染性腹膜炎的诊断和治疗提供一定的参考意义。 相似文献
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Cat scratch disease: a bacterial infection 总被引：21，自引：0，他引：21
D J Wear A M Margileth T L Hadfield G W Fischer C J Schlagel F M King 《Science (New York, N.Y.)》1983,221(4618):1403-1405
Histopathologic examination of lymph nodes from 39 patients with clinical and pathological criteria for cat scratch disease revealed delicate pleomorphic Gram-negative bacilli in 34 of the 39 nodes. They were within the walls of capillaries in or near areas of follicular hyperplasia and within microabscesses. They were best seen with the Warthin-Starry silver impregnation stain. Organisms in lymph node sections exposed to convalescent serum from three patients and to immunoperoxidase stained equally well with all three samples. The organisms did not react with hyperimmune sera to Legionella pneumophila nor to several species of Rickettsia. These bacilli appear to be the causative agents of cat scratch disease. 相似文献
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The concentration of tritiumlabeled adrenaline was determined in various areas of cat brain after intravenous infusion. It did not exceed that expected from the blood content of the tissue except in the hypothalamus, where small but significant amounts of H(3)-adrenaline were found. 相似文献
13.
Introduction of milk or corn oil into the duodenum of the cat evokes an increase in superior mesenteric blood flow (blocked by atropine), an inhibition of gastric and duodenal motility, and sedation. Cholecystokinin-pancreozymin mimics the mesenteric vascular effect of intraduodenal fat and seems to have a sedating action. 相似文献
14.
The methoxy analogs of epinephrine, norepinephrine, and dopamine manifest some activity on smooth muscle and blood pressure. Metanephrine is as potent as epinephrine on rabbit aortic strips. The same compounds demonstrate a lesser potency on rabbit duodenum preparation, while 3-methoxy-dopamine produces a slight contraction of the duodenum. Both metanephrine and 3-methoxy-dopamine have 15 to 25 percent of the pressor activity of the original nonmethylated catecholamines. Normetanephrine is 1/600 as active as norepinephrine on the blood pressure of the cat. 相似文献
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Quaternary salts of two triesters of p-dimethylaminothiophenol and phosphoric acid or thiophosphoric acid have effects similar to epinephrine on blood pressure, heart rate, and the nictitating membrane of the anesthetized cat. Two analogous compounds derived from phenol are depressor. None is a potent anticholinesterase. Further investigation is desirable because of the possibility of a relatively specific action on sympathetic ganglia. 相似文献
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Phenylhydrazine-induced anemia in the domestic cat results in an increase in minor, high oxygen affinity hemoglobin B components and an accompanying decrease in the major, low affinity B component. This change is accompanied by an unusually large increase in erythrocytic adenosine triphosphate and 2,3-diphosphoglycerate, a slight decrease in the oxygen affinity of whole blood, and a large decrease in the Hill constant. 相似文献
17.
Storage-body diameter and volume, and the number of molecules of serotonin contained in a storage body, were estimated for blood platelets. In the human, 5.23 x 10(5) molecules of serotonin are contained in a storage body 198 nanometers in diameter, while in the cat, 31.2 x 10(5) molecules of this amine are contained in a storage body 298 nanometers in diameter. 相似文献
18.
Transmission of simian acquired immunodeficiency syndrome (SAIDS) with blood or filtered plasma 总被引：4，自引：0，他引：4
M Gravell W T London S A Houff D L Madden M C Dalakas J L Sever K G Osborn D H Maul R V Henrickson P A Marx 《Science (New York, N.Y.)》1984,223(4631):74-76
Simian acquired immunodeficiency syndrome (SAIDS), a disease clinically and pathologically similar to acquired immunodeficiency syndrome in humans, was transmitted from diseased rhesus monkeys (Macaca mulatta) to normal monkeys by inoculation with heparinized whole blood or plasma that had been passed through filters of 0.45 micrometer pore size. This suggests that the causative agent is small and most probably a virus. No viruses, however, were isolated by standard cell culture techniques from the blood or filtered plasma which caused SAIDS. Both cellular and humoral immunity were markedly depressed in animals with advanced SAIDS. 相似文献
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猫抓病是一种由巴尔通体属细菌引起的人畜共患病,在世界范围内广泛流行。猫是巴尔通体的主要宿主,通过猫蚤在猫之间传播。猫抓伤或咬伤是该病从猫传播到人的主要途径。文章就该病的病原学、流行病学、临床表现、实验室检查、病变特点、诊断及治疗方法等作一综述。 相似文献
20.
Vasoactive intestinal polypeptide-like substance: the potential transmitter for cerebral vasodilation 总被引：9，自引：0，他引：9
In vitro pharmacological studies demonstrated that exogenously applied vasoactive intestinal polypeptide (VIP) relaxes the smooth muscle cells of cat cerebral arteries, whereas substance P constricts them. Ultrastructural-immunocytochemical techniques show that a VIP-like substance is present in the large granular vesicles of nonsympathetic nerve axons and terminals in the cerebral arterial walls. These results provide strong evidence in favor of the hypothesis that a VIP-like substance is the transmitter for vasodilation in cerebral blood vessels. 相似文献