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1.
WRKY转录因子作为植物特有的一类重要转录调控因子,广泛参与植物对生物和非生物胁迫的应答反应及形态发育和组织衰老等,已引起人们的关注。本文以前期克隆得到的毛白杨WRKY转录因子基因（命名为PtWRKY1）为研究对象,开展其基因枪介导洋葱表皮细胞瞬时表达、外源信号分子（水杨酸SA,茉莉酸MeJA,脱落酸ABA）诱导表达、转PtWRKY1基因烟草植株的烟草花叶病毒（TMV）接种实验以及抗病相关基因表达的实时荧光定量RT-PCR（qRT-PCR）分析等研究。结果表明,PtWRKY1基因编码蛋白定位于细胞核,SA、ABA及MeJA处理均可诱导PtWRKY1基因表达,但不同诱导因子对PtWRKY1表达量的影响存在一定差异;同时,TMV侵染实验发现外源PtWRKY1表达能增强转基因烟草抗TMV能力,qRT-PCR分析表明PtWRKY1基因及膜保护性酶（POD,SOD,CAT）基因均受TMV诱导而增强表达。本文研究结果可为后续开展毛白杨转录因子PtWRKY1的功能鉴定及应用奠定基础。 相似文献
2.
F-box蛋白家族在植物抗逆响应中的作用机制 总被引：1，自引：0，他引：1
SCF复合体泛素连接酶E3介导的泛素化蛋白降解是翻译后水平上对生命进程进行调控的一个重要方式。它的关键组分F-box蛋白负责识别被降解的靶底物蛋白。植物F-box基因家族成员众多,极具多样性。F-box蛋白N端常含F-box基序,C端常为蛋白互作保守结构域,该结构具多样性,可识别不同底物,是F-box蛋白分类的依据。研究表明,F-box蛋白参与调控植物的许多生命进程,包括抗逆反应。本文就近年来F-box蛋白在植物抗逆反应中的作用机制进行总结。F-box蛋白大多以SCF复合体泛素连接酶E3介导的泛素化蛋白降解目标蛋白的方式调控抗逆反应,也有不依赖形成SCF复合体的方式行使功能,不少F-box蛋白参与了植物激素信号传导,通过调控转录因子活性而改变下游基因的表达,由此影响了植物的抗逆反应。基因表达谱的生物信息学预测表明,大多数F-box基因参与了植物抗逆反应,目前只有其中一小部分已报道了其抗逆调节功能。在此综述了这些F-box蛋白在植物抗逆胁迫中的研究进展。在干旱和盐碱胁迫反应中,F-box基因常通过影响植物激素脱落酸、乙烯等植物激素信号传导而调控抗逆。由于干旱和盐碱胁迫具协同性,不少F-box基因同时参与抗旱和抗盐碱胁迫,但调节方式有所不同,一些F-box基因对抗干旱和盐碱的反应具协同性,从总体上调控植物的渗透胁迫和离子毒害反应;而另一些F-box基因对干旱和盐胁迫反应的调节作用相反,它们可能在植物抗逆的精细调节中起作用。在低温胁迫反应中,F-box蛋白可调节植物抗低温的CBF信号途径。在生物胁迫反应中,F-box基因常通过影响植物激素茉莉酸和水杨酸途径来调控抗病,病原菌也以攻击植物SCF复合体使植物致病。此外,植物激素信号途径之间相互作用,共同影响抗逆反应。 相似文献
3.
ATP结合盒转运蛋白G亚家族（ABCG）在植物体内的物质运输过程中发挥着重要作用。为探究毛竹（Phyllostachys edulis）中ABCGs的分子特征、表达模式及转录因子对PeABCG15的调控关系，本研究利用生物信息学方法鉴定毛竹ABCG基因家族成员，对其基因结构、编码蛋白的理化性质和系统进化等进行系统分析，通过实时荧光定量PCR(qRT-PCR)技术对毛竹ABCG基因表达模式进行研究，借助酵母单杂交验证转录因子与ABCG基因的调控关系。结果表明，在毛竹基因组中共鉴定到77个ABCG基因（PeABCG1～PeABCG77），其启动子中含有多种激素和非生物胁迫响应元件，其中脱落酸响应元件ABRE最多，出现在74个基因的启动子中。系统进化分析发现，PeABCGs分为白棕色复合体（WBC）和多效性耐药复合体（PDR）两个亚组，与水稻的亲缘关系较近。qRT-PCR结果显示，在脱落酸（ABA）处理后，7个与ABA运输相关的PeABCGs中有6个呈上调表达趋势，而未检测到PeABCG34表达；在低温处理条件下，7个PeABCGs均受到诱导表达；在干旱处理条件下，有2个PeABCGs的表达... 相似文献
4.
植物水分胁迫下功能蛋白的研究进展 总被引：16，自引：0，他引：16
介绍了水分胁迫下的几种功能蛋白（水分通道蛋白、渗调物生物合成的关键酶、LEA蛋白等）的特性,以及几种主要的渗透调节物质（脯氨酸、甜菜碱、多胺）在植物体内的定位、生理功能和它们在植物体内的生物合成途径以及合成酶类的相关基因。 相似文献
5.
干旱、高盐及低温胁迫下植物生理及转录因子的应答调控 总被引：2，自引：1，他引：1
干旱、高盐及低温等非生物胁迫是限制植物生长发育的主要环境因子。这些环境胁迫因子通常导致植物体内生理代谢改变,并参与非生物胁迫调控转录因子的差异表达。植物抵御上述非生物逆境的能力与转录因子调控逆境相关功能基因的表达密不可分。近年来,发掘植物非生物胁迫相关转录因子的功能及揭示转录因子介导植物非生物胁迫响应的调控机制,已成为植物营养分子生物学关注的热点之一。因此,了解植物非生物胁迫下的生理应答及转录因子参与的调控机制,对建立植物适应性改良途径具有重要科学意义。本文从干旱、高盐和低温三方面阐述了非生物胁迫下植物生理生化的适应性变化,概述了MYB、bZIP、AP2/EREBP、WRKY和NAC五类与植物抗逆相关的转录因子的结构与功能特征,着重论述了转录因子介导植物抵御非生物胁迫的分子调控机制。植物遭遇非生物胁迫时,通常表现为生长速率、叶面积和叶片数量下降,蒸腾及光合速率降低。同时,植物体内活性氧逐渐累积,使细胞膜脂过氧化程度加剧,造成细胞损伤。为适应不利环境,在生理上植物表现为体内抗氧化酶活性增强,渗透调节物数量增多;在分子水平上,植物对非生物胁迫适应性的增强,通常与转录因子识别抗逆基因启动子特异性元件及调控逆境防御基因的转录有关。本文对于深入阐明干旱、高盐及低温胁迫下植物生理生化应答与转录因子的分子调控机制提供了全新的科学启示。 相似文献
6.
脱落酸(ABA)是一种重要的植物激素,调节多种植物生理过程,并在植物应答逆境胁迫的信号转导中起重要作用。结构分子生物学分析发现,PYL蛋白具有结合ABA的活性中心。在非胁迫条件下,ABA水平较低,2C型蛋白磷酸酶(PP2C)与蔗糖非酵解型蛋白激酶2(SnRK2)结合,催化其去磷酸化而抑制其活性;在胁迫条件下,ABA水平升高,促进PYL与PP2C结合而抑制其磷酸酶活性,SnRK2靠自身磷酸化激活,又催化碱性亮氨酸拉链(b ZIP)、碱性螺旋-环-螺旋(b HLH)等类型转录因子,调控下游抗性相关基因的表达,也可由SnRK2直接催化下游抗性相关蛋白磷酸化。本文概述了在ABA胁迫下,PYL,PP2C和Sn PK2作用机制的相关研究进展,并建议将PYL、PP2C和SnRK2分别称做ABA信号转导途径的直接受体、第二信使和第三信使,建立玉米中复杂的脱落酸信号上淳传递网络。 相似文献
7.
高等植物对渗透胁迫的基因表达 总被引：8，自引：0，他引：8
干旱、低温、盐渍等环境条件严重限制植物的生长和作物的产量。通过分析植物对环境胁迫的基因表达，人们可以利用基因工程手段获得抗逆性强的作物新品种。本文从以下几个方面介绍有关植物对渗透胁迫的基因表达调控及基因工程的研究进展：（１）植物对渗透胁迫的生理生化反应；（２）渗透胁迫下植物ＬＥＡ基因的表达；（３）水分胁迫下ＡＢＡ对基因表达的调控；（４）耐渗透胁迫的植物基因工程。 相似文献
8.
水通道蛋白（AQPs）是植物中重要的水分转运蛋白，在植物对抗干旱胁迫中发挥着重要功能。为明确野生大豆水通道蛋白在植株抗旱反应中的分子功能，本研究对野生大豆（Glycine soja）中的水通道蛋白的蛋白结构及其在干旱胁迫下的表达模式进行分析，并对筛选的AQPs基因功能进行验证。研究鉴定了野生大豆中69个AQPs蛋白，其中10个在干旱胁迫的高耐野生大豆材料中表达差异达到显著水平。筛选到GsPIP2;7在干旱胁迫后与对照相比显著上调，克隆GsPIP2;7到过表达载体中并侵染转化拟南芥（Arabidopsis thaliana），通过转基因后代筛选获得了稳定表达的T3代转基因拟南芥。与野生型拟南芥相比，转基因植株在干旱胁迫下根系总长度、叶干重、根干重、根冠比和单株叶面积显著增加，过氧化物酶（POD）、过氧化氢酶（CAT）和超氧化物歧化酶（SOD）活性显著提升，丙二醛（MDA）含量显著降低，表现出较强的耐旱能力。本研究结果说明GsPIP2;7对拟南芥耐旱具有正向调控作用，为明确野生大豆耐旱机制奠定了理论基础。 相似文献
9.
我国北方地区石灰性土壤上缺锌与干旱限制因子同时存在,影响作物生长和产量提高。本文综述了干旱胁迫影响土壤锌的有效性和植物对锌吸收利用的可能机制:土壤中锌不同形态间的转化与化学行为、根际土壤中锌的移动性、植物根系形态和生理反应、植物体内锌的运输以及植株对锌的需求等在水分胁迫下都可能发生变化。植物锌营养状况可能与植物气孔开闭、活性氧代谢和基因转录因子-锌指蛋白的形成等关系密切,从而影响植物对水分的吸收利用和对干旱胁迫的适应。 相似文献
10.
胡萝卜DcDREB-A6亚族转录因子基因的克隆与非生物胁迫响应分析 总被引：1，自引：0，他引：1
干旱响应元件结合蛋白(dehydration responsive element-binding protein,DREB)类转录因子是干旱应答元件的结合蛋白,在植物响应非生物胁迫时发挥重要作用。本研究基于胡萝卜(Daucus carota L.)转录组数据,检索和拼接获得一个胡萝卜DREB类转录因子基因序列,并根据其序列设计引物,采用RTPCR方法从黑田五寸获得该胡萝卜转录因子基因DcDREB-A6(GenBank登录号:KM386646)。序列分析显示,来源于胡萝卜中的DcDREB-A6基因含有993 bp的开放阅读框,编码330个氨基酸。氨基酸组成成分、理化性质、亲水性/疏水性和三级结构分析结果显示,胡萝卜DcDREB-A6转录因子蛋白质分子量为36.68 kD,等电点为6.00,属于亲水性蛋白。进化树分析显示,DcDREB-A6属于APETLA 2/乙烯反应元件结合蛋白(AP2/ERF)家族转录因子中DREB亚族中的A6组。空间结构表明,该转录因子结构域具有典型的植物AP2/ERF家族转录因子的结构特征,有1个α螺旋和3个β折叠。实时定量荧光PCR证实,胡萝卜DcDREB-A6转录因子基因受高温、低温和高盐胁迫诱导,分别在高温、低温处理4和2 h后表达量增加2倍左右;盐胁迫对其影响较大,处理8 h时,基因表达量增加4.6倍;而干旱胁迫下该基因表达变化幅度相对较小。胡萝卜DcDREB-A6转录因子基因能够响应高温、低温、干旱和高盐等非生物胁迫,本研究结果有助于进一步开展胡萝卜DREB类转录因子的逆境调控研究。 相似文献
11.
【目的】以富士（Fuji）、 秦冠（Qinguan）嫁接在平邑甜茶（Malus hupehensis Rehd.）上的当年生盆栽苗为试验材料,采用砂培方法,研究了缺氮胁迫和干旱对富士和秦冠生长情况、 光合参数、 植株各部位氮磷钾含量及氮素利用效率的影响,分析比较了低氮干旱条件下富士和秦冠生长及氮素利用的差异,以期为果树生产高效肥水利用提供理论指导。【方法】试验共设四个处理: 正常氮正常水（ZZ）、 低氮正常水（DZ）、 正常氮干旱（ZG）、 低氮干旱（DG）。氮素和水分均设置两个水平,分别为正常氮（6 mmol/L NO-3-N）、 低氮（0.3 mmol/LNO-3-N）、 正常供水（保持盆中砂子相对含水量为饱和含水量的80%~85%）、 干旱处理（保持盆中砂子相对含水量为饱和含水量的60%~65%）。【结果】富士和秦冠的生物量（茎和叶）、 株高茎粗等生长指标以及光合速率、 气孔导度、 蒸腾速率均为正常氮正常水（ZZ）＞低氮正常水（DZ）＞正常氮干旱（ZG）＞低氮干旱（DG）,并且相对应处理下秦冠的以上指标均高于富士;正常供水下,缺氮处理使富士、 秦冠的根冠比比正常氮处理均有所增加,富士提高了2.05%,秦冠提高了22.40%。富士和秦冠的氮、 磷、 钾含量均表现出正常氮正常水（ZZ）＞低氮正常水（DZ）＞正常氮干旱（ZG）＞低氮干旱（DG）; 氮、 钾元素含量在植株各部位的分布顺序依次是叶＞根＞茎,磷元素则是根＞叶＞茎;光合氮素利用效率（PNUE）和氮素利用效率表现为秦冠处理之间差异极显著,富士处理之间差异不显著;秦冠的PNUE和NUE明显高于富士,在低氮正常水（DZ）处理下,秦冠氮肥利用率比富士高42.07%,在低氮干旱（DG）处理下高64.14%;低氮胁迫下富士和秦冠的NUE显著提高,并且秦冠提高的幅度高于富士。【结论】施用氮肥能够显著提高富士与秦冠的干物质量,同等水肥条件下,秦冠生长优于富士;水分亏缺会减少叶片对氮的吸收,干旱条件下适度增施氮肥,可提高果树的抗旱能力;低氮干旱胁迫下秦冠的生长指标、 光合指标及氮素利用效率指标均优于富士,表现出较强的抗低氮干旱胁迫的能力。 相似文献
12.
Kyle Devey Michael Mucalo Gordon Rajendram Joseph Lane 《Communications in Soil Science and Plant Analysis》2015,46(4):72-80
Laser-induced breakdown spectroscopy (LIBS) is a new technique for the analysis of plant material. This study investigates the application of LIBS to pasture-based plant samples. The LIBS measurements were obtained from pelletized pasture samples (100 samples) that had been also analyzed by inductively coupled plasma–optical emission spectroscopy (ICP-OES) following microwave digestion for calibration and comparison purposes. Comparisons for elements sodium (Na), potassium (K), magnesium (Mg), calcium (Ca), manganese (Mn), iron (Fe), copper (Cu), zinc (Zn), boron (B), phosphorus (P), and sulfur (S) showed that LIBS could be used for almost all the standard profile total elements with concentrations down to low mg/kg levels (observed error of Na: 0.024 percent, K: 0.18 percent, Mg: 0.016 percent, Ca: 0.073 percent, P: 0.017 percent, Mn: 31 mg/kg, Fe: 150 mg/kg, Zn: 6.6 mg/kg, and B: 1.1 mg/kg). Elemental analysis at less than mg/kg levels was not possible using LIBS. The elements S and Cu were particularly difficult to analyze with reliability using LIBS at the concentration levels found in the plant samples. Replacing microwave digestion and subsequent ICP analysis with a direct analysis of dried plant samples using LIBS has the potential to improve the productivity and reduce the cost of testing. 相似文献
13.
《中国生态农业学报》2014,(8)
正The Center for Agricultural Resources Research(CARR),the Institute of Genetics and Developmental Biology(IGDB),Chinese Academy of Sciences,invites applicants for several research group leader positions.CARR is one of the research organizations in Chinese Academy of Sciences(CAS).We seek nominations and applications from individuals who have expertise and a record of accomplishment in research areas related to ecology,agro-hydrology, 相似文献
14.
M.A. Callaham Jr. J.M. BlairT.C. Todd D.J. KitchenM.R. Whiles 《Soil biology & biochemistry》2003,35(8):1079-1093
The responses of tallgrass prairie plant communities and ecosystem processes to fire and grazing are well characterized. However, responses of invertebrate consumer groups, and particularly soil-dwelling organisms, to these disturbances are not well known. At Konza Prairie Biological Station, we sampled soil macroinvertebrates in 1994 and 1999 as part of a long-term experiment designed to examine the effects and interactions of annual fire, mowing, and fertilization (N and P) on prairie soil communities and processes. For nearly all taxa, in both years, responses were characterized by significant treatment interactions, but some general patterns were evident. Introduced European earthworms (Aporrectodea spp. and Octolasion spp.) were most abundant in plots where fire was excluded, and the proportion of the total earthworm community consisting of introduced earthworms was greater in unburned, unmowed, and fertilized plots. Nymphs of two Cicada genera were collected (Cicadetta spp. and Tibicen spp.). Cicadetta nymphs were more abundant in burned plots, but mowing reduced their abundance. Tibicen nymphs were collected almost exclusively from unburned plots. Treatment effects on herbivorous beetle larvae (Scarabaeidae, Elateridae, and Curculionidae) were variable, but nutrient additions (N or P) usually resulted in greater densities, whereas mowing usually resulted in lower densities. Our results suggest that departures from historical disturbance regimes (i.e. frequent fire and grazing) may render soils more susceptible to increased numbers of European earthworms, and that interactions between fire, aboveground biomass removal, and vegetation responses affect the structure and composition of invertebrate communities in tallgrass prairie soils. 相似文献
15.
Grete Skrede Berit Karoline Martinsen Anne-Berit Wold Stein-Erik Birkeland Kjersti Aaby 《Acta Agriculturae Scandinavica, Section B - Plant Soil Science》2013,63(3):193-208
Abstract A 3-year study was carried out to investigate quality parameters in 14 tree fruit and berry species grown in southern Norway. The species were blueberry, apple, aronia, sour cherry, sweet cherry, red raspberry, strawberry, blackcurrant, gooseberry, red currant and elderberry, harvested along with wild bilberry, cloudberry and lingonberry. Significant differences between species were identified for all quality parameters. The coefficient of variation between species was lowest for pH (12.5%), dry matter (18.9%) and soluble solids (25.3%), followed by titratable acids (59.3%), total phenolics (83.8%), antioxidant capacity FRAP (85.7%) and antiradical power by the DPPH-assay (97.8%), total monomeric anthocyanins (132%) and ascorbic acid (137%). Average coefficient of variation within species were lower and ranged from 4 (pH) to 62% (ascorbic acid). Only the FRAP values were significantly affected by harvesting year with lower levels in 2004 than in 2005 and 2006. There were significant interactions between species and harvesting year for dry matter, soluble solids, pH, ascorbic acid and FRAP. The results indicate generic ranges in composition within species independent upon growing location and climate, and the composition of the tree fruits and berries is not likely to deviate from these ranges. It is concluded that desirable composition of tree fruits and berries and their products should primarily be achieved by selection among species rather than searching fors broadened variation within individual species. 相似文献
16.
《Communications in Soil Science and Plant Analysis》2012,43(7):865-877
Potassium (K) fixation and release in soil are important factors in the long-term sustainability of a cropping system. Changes in K concentration and characteristics of K fixation and release in rhizosphere and nonrhizosphere soils in the rapeseed (Brassica napus L.)–rice (Oryza sativa L.) rotation were investigated using a rhizobox system. The concentrations of different forms of K in both rhizosphere and nonrhizosphere soils decreased with plants compared to without plants, regardless of K fertilizer application. Potassium uptake by crops mainly came from the rhizosphere soil. In the treatment without K fertilizer (–K), the main form of K supplied by the soil to the crops was 1.0 mol L?1 nitric acid (HNO3) nonextractable K, followed by nonexchangeable K, and then exchangeable K. In the treatment with K fertilizer (+K), the main K forms supplied by the soil to the crops were exchangeable K and nonexchangeable K. The amount and rate of K fixation after one cycle of the rapeseed–rice rotation was greater in rhizosphere soil than in nonrhizosphere soil. The amount and rate of K fixation of soil in the +K treatment were significantly less than in the –K treatment. The cumulative amounts of K released with 1.0 mol L?1 ammonium acetate (NH4OAc) and 1.0 mol L?1 HNO3 extraction increased with the increasing numbers of extractions, but the K-releasing power of soil by successive extraction decreased gradually and finally became almost constant. The release of K was less in rhizosphere soil than in nonrhizosphere soil. The release of K in the +K treatment was similar to that in the –K treatment in rhizosphere soil, but the K release in nonrhizosphere soil was greater with the +K than the –K treatment. Overall, the information obtained in this study will be helpful in formulating more precise K fertilizer recommendations for certain soils. 相似文献
17.
Electron donor and pH relationships for biologically enhanced dissolution of chlorinated solvent DNAPL in groundwater 总被引：3，自引：0，他引：3
Biologically enhanced dissolution offers a method to speed removal of chlorinated solvent dense non-aqueous-phase liquid (DNAPL) sources such as tetrachloroethene (PCE) and trichoroethene (TCE) from aquifers. Bioremediation is accomplished by adding an electron donor to the source zone where fermentation to intermediates leading to acetic acid and hydrogen results. The hydrogen and possibly acetic acid are used by dehalogenating bacteria to convert PCE and TCE to ethene and hydrochloric acid. Reductive dehalogenation is thus an acid forming process, and sufficient alkalinity must be present to maintain a near neutral pH. The bicarbonate alkalinity required to maintain pH above 6.5 is a function of the electron donor: 800 mg/L of bicarbonate alkalinity is sufficient to achieve about 1.2 mM TCE dechlorination with glucose, 1.7 mM with lactate, and a much higher 3.3 mM with formate. Laboratory studies indicate that in mixed culture, formate can be used as an electron donor for complete conversion to ethene, contrary to pure cultures studies indicating it cannot. Various strategies can be used to add electron donor to an aquifer for DNAPL dehalogenation while minimizing pH problems and excessive electron donor usage, including use of injection-extraction wells, dual recirculation wells, and nested injection-extraction wells. 相似文献
18.
Aleš Lebeda Ivana Doležalová Eva Křístková Barbora Mieslerová 《Genetic Resources and Crop Evolution》2001,48(2):153-164
Staff members of the Department of Botany of Palacký University in Olomouc and Gene Bank Department – Workplace Olomouc, Research Institute of Crop Production in Prague, Czech Republic, conducted an expedition in seven European countries (Austria, Czech Republic, France, Germany, Italy, the Netherlands, Switzerland) in August/September 1999 to collect wild Lactuca spp. germplasm and study its geographic distribution, ecology and biodiversity. During the mission, more than 600 locations were visited resulting in the collection of 602 seed samples (accessions) of wild Lactuca species and 13 seed samples of related genera (Chondrilla and Mycelis). Lactuca serriola f. serriola, L. serriola f. integrifolia, L. saligna and L. viminea subsp. chondrilliflora were prevalent in southern Europe (Italy, France), however, only L. serriola was common in central and western Europe (Austria, Czech Republic, Germany, Netherlands, Switzerland). The greatest diversity of Lactuca species was found in France, where also the most seed samples (165) were collected. The most characteristic habitats with a high density of Lactuca spp. populations were observed along roads and highways, grassy ditches, ruderal communities, and dust-heaps. Natural infections by powdery mildew (Erysiphe cichoracearum) and downy mildew (Bremia lactucae) on some wild Lactuca spp. were observed. Recent observations concerning the geographic distribution, population structure, habitats, and natural occurrence of diseases of Lactuca spp. are discussed. This assemblage of genetic resources of Lactuca spp. can serve as the basis of future studies of species diversification, spatial population structure, plant microevolution, domestication processes, and genetic variability of host-parasite interactions. 相似文献
19.
Y. Bashan J. J. Bustillos L. A. Leyva J.-P. Hernandez M. Bacilio 《Biology and Fertility of Soils》2006,42(4):279-285
Inoculation of wheat seedlings with the plant growth-promoting bacterium Azospirillum brasilense Cd was immobilized in alginate microbeads and, without applying any stress, significantly increased the quantity of several
photosynthetic pigments, such as chlorophyll a, chlorophyll b, and the auxiliary photoprotective pigments violaxanthin, zeaxanthin, antheroxanthin, lutein, neoxanthin, and β-carotene.
This resulted in greener plants with no apparent visible stress. After monitoring the quantity of photosynthetic pigments
for 4 weeks, we observed that inoculated plants had higher quantities of pigments in shoot and stem. The greatest difference
in the quantity of all pigments between inoculated and noninoculated plants occurred in the first week of growth. Regardless
of treatment, the quantity of pigments in stems was three to four times less than the quantity of these pigments in shoots.
Application of Azospirillum, either as liquid inoculant or as alginate microbeads, did not alter the positive effect of the bacteria on pigment production
or the positive response of the plants towards A. brasilense Cd inoculation. 相似文献
20.
Three slow-growing legume trees used for desert reforestation and urban gardening in the Sonoran Desert of Northwestern Mexico
and the Southwestern USA were evaluated whether their growth can be promoted by inoculation with plant growth-promoting bacteria
(Azospirillum brasilense and Bacillus pumilus), unidentified arbuscular mycorrhizal (AM) fungi (mainly Glomus sp.), and supplementation with common compost under regular screenhouse cultivation common to these trees in nurseries. Mesquite
amargo (Prosopis articulata) and yellow palo verde (Parkinsonia microphylla) had different positive responses to several of the parameters tested while blue palo verde (Parkinsonia florida) did not respond. Survival of all tree species was over 80% and survival of mesquite was almost 100% after 10 months of cultivation.
Inoculation with growth-promoting microorganisms induced significant effects on the leaf gas exchange of these trees, measured
as transpiration and diffusive resistance, when these trees were cultivated without water restrictions. 相似文献