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灯盏花多倍体植株的诱导培养研究 总被引：1，自引：0，他引：1
以二倍体灯盏花种子为材料,进行了分别经0.1%的秋水仙素诱导处理12 h、24 h、36 h,接种于培养基培养灯盏花多倍体幼苗的实验研究。结果表明:用0.1%秋水仙素处理灯盏花种子12 h的诱变效果最佳,其所培养幼苗的诱变率为38.9%。通过采用不定芽继代培养的途径对灯盏花幼苗多倍体植株进行了分离与稳定培养,获得了稳定的灯盏花幼苗四倍体植株。与二倍体灯盏花植株相比,四倍体灯盏花植株表现为叶片较厚、较大、叶色较深,气孔也较大,其染色体数目为2n=4x=36。 相似文献
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秋水仙素对离体培养辣木多倍体的诱导 总被引：1，自引：0，他引：1
以印度辣木栽培品种PKM(Moringa oleifera PKM-1)一年生植株茎尖为外植体,采用秋水仙素结合组织培养技术诱导多倍体的方法,研究了秋水仙素浓度及处理时间对诱导染色体倍性变异的影响,以及不同外源植物生长调节剂浓度对茎段培养的增殖影响,结果表明:辣木芽初代培养的最适培养基为MS+6-BA2.0mg/L+NAA0.2mg/L,不定芽增殖的最佳培养基为MS+6-BA0.6mg/L,增殖系数为3.57,用0.1%秋水仙素处理24h左右,可诱导获得四倍体再生植株。 相似文献
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白花泡桐同源四倍体的诱导 总被引：2，自引：0，他引：2
将预培养不同时间的白花泡桐组培苗叶片分别放置到含有不同浓度秋水仙素的双层(液体和固体)MS NAA 0.1 mg·L-1 BA 18 mg·L-1培养基上进行染色体加倍试验,通过根尖染色体计数和叶片单细胞相对DNA含量测定进行变异植株的倍性分析.结果表明:秋水仙素浓度对叶片存活率和芽诱导的影响达到极显著水平,对四倍体诱导的影响不显著;秋水仙素处理外植体时间对四倍体诱导率影响显著,外植体预培养时间对芽诱导率和四倍体诱导率的影响达到极显著水平.在9个试验组合中,用5 mg·L-1秋水仙素处理预培养12 d白花泡桐叶片72 h时,四倍体诱导率最高可达20.0%.诱导出的四倍体植株叶片较二倍体增大、增厚,叶片单个气孔器变大,叶片气孔密度变小. 相似文献
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We developed procedures for the micropropagation of Juniperus oxycedrus L. using shoot apices or nodal segments from mature plants. Of the media and explants examined, best culture establishment was obtained with shoot apices cultured on modified Schenk and Hildebrandt medium (SH medium) without growth regulators; however, shoot multiplication was only achieved when shoot apices isolated from shoots grown on SH medium without growth regulators were subcultured on SH medium containing 0.5 micro M benzyladenine. None of the auxins and methods tested for root induction provided satisfactory results. 相似文献
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在油茶新造林地水平带边缘利用3年生截干苗开展美丽胡枝子植篱种植试验。试验表明:在下坡立地条件较好的地段,无论萌枝数量、生长高度、基径、冠幅和生物产量各项生长因子,都要高于上坡立地条件较差的地段,其中下坡地段的生物量要比上坡高48.31%;通过刈割与不刈割对美丽胡枝子生长影响的研究表明,年底刈割有利于促进美丽胡枝子的树高生长和萌枝数量的增加,但对基部直径和冠幅的生长有一定的影响,刈割获取的地上部分生物量比不刈割增加46.88%,具有越刈越旺的特点。美丽胡枝子在新造油茶林地水平带边缘植篱的成功种植,可结合冬季垦复抚育覆盖林地,将会对油茶新造林地水土保持、固土护坡和改良土壤等起着积极的生态保育作用。 相似文献
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以洋紫荆(Bauhinia variegata)成熟种子为试验材料,采用质量浓度为0.5、1.0、1.5、2.0 mg/mL的秋水仙素溶液分别处理12、24、36和48 h,并以蒸馏水浸泡种子作为对照,观察测定其种子萌发特性及幼苗生长指标.结果表明,随着秋水仙素质量浓度的增加,种子发芽率逐渐降低,2.0 mg/mL溶液使大部分种子致死,0.5、1.0 mg/mL质量浓度秋水仙素处理24 h有利于种子萌发;种子胚根的伸长随着秋水仙素质量浓度的增加也受到不同程度地抑制;秋水仙素对发芽种子胚芽的发育有一定的抑制作用;不同质量浓度秋水仙素处理后第7个月,各处理间苗高与地径差异均极显著(P<0.01),质量浓度为1.0 mg/mL,处理36 h时,苗高和地径都最大;当秋水仙素质量浓度为2.0 mg/mL时,对叶片平均分裂角度有一定促进作用. 相似文献
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为了获得大果、质优的多倍体品种,以猕猴桃‘琼露’无菌苗叶柄为材料,分别利用抽滤灭菌的0.05%、0.10%的秋水仙素溶液浸泡3、4、5 h,培养再生植株,利用流式细胞仪进行倍性鉴定。结果表明:0.05%的秋水仙素处理3 h,存活率和再生频率分别达到85.19%和82.61%,低于对照;当0.10%的秋水仙素处理5 h时,其存活率和再生频率分别降低至36.00%和44.44%。其中,以0.05%的秋水仙素处理4 h诱导效果最佳,变异率为28%。倍性鉴定结果表明,共获得四倍体植株7株,嵌合体5株。多倍体与二倍体相比,叶片变大,叶面变厚,节间变短,与二倍体差异显著。 相似文献
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用秋水仙碱处理离体的非洲菊愈伤组织与丛生芽,研究了4种不同浓度的秋水仙碱对非洲菊芽增殖和染色体加倍的影响.结果表明:以0.05%的秋水仙碱溶液处理12 h效果较好,变异率达38.46%;叶片表皮细胞的气孔数量减少、体积增大以及植株叶大根粗等外部形态特征均可作为鉴别非洲菊多倍体的间接证据. 相似文献
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在全球环境保护基金的资助下,对保护区种子植的区系进行了详细的调查。收集了大量资料,采集标本２１２８号。经鉴定分析,其区系的基本特征是：种类丰富,拥有种子植物９５０种,隶属１０５科４３３属,区系起源古老;地理成分复杂;珍稀濒危植物较多。 相似文献
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以金线莲野生植株茎段为外植体,在无菌系建立、增殖和生根培养时进行了适宜培养基的筛选试验,结果表明:以MS为诱导培养基建立无菌系效果较好;以MS+KT 1.0 mg/L(单位下同)+IAA 0.2 mg/L+0.15%活性炭能有效增殖,匍匐茎节部白色芽点多,易生成片状丛生芽块;在1/2 MS+IAA 2.0+0.15%活性炭的培养基上生根率达95%以上。同时开展了组培苗移栽试验,以泥炭土∶腐殖土∶蛭石=1∶1∶1配比为组培苗移栽适宜基质,在可控的设施环境下或透光度50%左右的林下移栽,成活率达87.6%。 相似文献
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Karoly Redei Zoltan Osvath-Bujtas Irina Veperdi 《中国林学(英文版)》2006,8(4):47-50
Black locust (Robinia pseudoacacia L.) is one of the most important stand-forming tree species in Hungary and its importance is increasing in many countries. The main aim of the discussed new selection programme is to identify black locust clones with good performance and good form for setting up clonal seed orchards. As a result of selection programme 16 new black locust clones have been improved. In spring 2002 a black locust seed orchard was established with the newly selected clones. About 40% of the plants can be considered to belong to the height growth rate class 1 and 2. Hungary was the first country where micropropagated black locust planting material was used for seed orchard establishment. 相似文献
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For improving seed germination of Prosopis koelziana and Prosopis juliflora, different treatments of seeds were conducted, including scarification with sulfuric acid 98% for 10 and 15 min, sandy paper,
hot water for 5 and 10 min, potasium nitrate 0.1%, gibberellic acid at 250 mg·L−1 and 500 mg·L−1 and combinational treatment of scarification with gibberellic acid of 250 mg·L−1 and 500 mg·L−1. The results show that scarifications with sandy paper and sulfuric acids 98% were the most effective treatments on breaking
seed dormancy and seed germination induction. Scarification with sulfuric acid 98% for 15 min was the best treatment. According
to the positive effect of scarification and lack of reaction of seeds against KNO3 and gibberellic acid, the kind of seed dormancy was determined as exogenous. 相似文献
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Legesse Negash 《Agroforestry Systems》2002,56(1):39-46
Erythrina brucei Schweinf. emend. Gillett (Papilionaceae),a tree species that is endemic to Ethiopia, is known to possess useful agroforestry attributes, but these attributes have not been quantified. To achieve this, propagation of the species, growth rate of young trees and their impact on barley (Hordeum vulgare L.), and leaf-nutrient concentrations were examined. Germination of seeds planted in plastic pots varied from 73 to 93%, and direct seeding in the field yielded 83%germination. Branch cuttings obtained from 2 to 3-year-old stock plants (n =150) resulted in 70 to 80% rooting within 8 to 12 weeks after setting cuttings into the rooting medium. Considerable nodulation occurred on leaves one year after planting out seedlings and stecklings. Leaf concentrations of Mg, N,P, and S were significantly lower in nodulated than in the non-nodulated leaves. On the other hand, leaf Fe concentration was higher in nodulated than in the non-nodulated leaves. There was no significant difference in Ca and K contentsbetween the nodulated and non-nodulated leaves. E. brucei-treatment significantly increased tiller and seed numbers, as well as seed weight. However, thousand-grain weight and grain size were similar in harvests from E. brucei-treated and the control plots. The results indicate that the species is easy to propagate by seed and vegetative means, grows fast, is desirable for intercropping, and has relatively high leaf-nutrient concentration.This revised version was published online in November 2005 with corrections to the Cover Date. 相似文献
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深色有隔内生真菌在寒温带地区的研究在国内还属空白。本研究首次对内蒙古大兴安岭兴安落叶松林下笃斯越橘、越橘、杜鹃和杜香的DSE真菌进行了研究。结果表明,这4种杜鹃花科植物都有DSE真菌定殖,但DSE定殖率都小于10%。DSE菌根定殖率与全氮呈极显著负相关。依据菌落形态特征和菌丝体的显微结构特征将这4种植物的根样中分离得到的187个DSE菌株分为15个菌株类型,并对这4种植物进行回接实验,其中有8种DSE菌株类型可以定殖这4种植物,8个菌株类型属于子囊菌门的Phialocephala属,分属4个种。 相似文献
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对大花蕙兰的无菌播种及组培快繁技术进行了相关研究。结果表明:大花蕙兰无菌播种采用0.1%升汞溶液灭菌10 min能取得较好的效果;改良MS+6-BA1.0 mg/L+NAA 0.5 mg/L培养基对假鳞茎诱导原球茎效果较好,其原球茎诱导率可达86%;原球茎增殖的最佳培养基为改良MS+NAA 0.5 mg/L,此配方对大花蕙兰的壮苗生根同样合适;采用固、液培养基交替培养(振荡转数100～110 r/min)能有效地促进原球茎增殖;移栽基质配比为腐殖土∶棉籽壳=1∶1时,移栽苗成活率可达95%。 相似文献