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1.
Comparative Study on Antioxidative System in Normal and Vitrified Shoots of Populus suaveolens in Tissue Culture 总被引：1，自引：0，他引：1
To explore the physiological and biochemical mechanism of the occurrence of vitrified shoots of Populus suaveolens in tissue culture, the changes in water, chlorphyll, lignin, H202, phenylalanine ammonialyase (PAL), malonaldehyde (MDA), protective enzymatic systems, and some key enzymes involved in the ascorbate- glutathione cycle were comparatively studied in both normal and vitrified shoots of P suaveolens. The results show that the lower activities of peroxidase (POD), catalase (CAT), ascorbate peroxidase (APX), dehydroascorbate reductase (DHAR), glutathione reductase (GR) and PAL, and the less contents of chlorphyll, lignin, ascorbate (ASA) and reduced glutathione (GSH) as well as the lower ratios of ASA / DHA and GSH / GSSG are observed in vitrified shoots than in normal ones during the whole culture period, While in comparison with normal shoots, the higher activity of superoxide dismutase (SOD) and the more concentrations of water, H2O2, MDA, dehydroascorbate (DHA) and oxidized glutathione (GSSG) are found in vitrified shoots. Statistical analysis indicates that the enhanced activity of SOD and the decreased activities of CAT and POD as well as some enzymes involved in the ascorbate-glutathione cycle might be closely correlated to the accumulation of H202. The less regeneration of ASA and GSH and the lower capacity of the ascorbate-glutathione cycle observed in vitrified shoots might be due to a significant decrease in APX, MDAR, DHAR and GR activities and a decline in redox status of ASA and GSH. The decreases in chlorphyll content might result in a decline in photosynthesis. The lower activities of POD and PAL could result in the decrease of lignin synthesis and cell wall ligination, which might be the key factor leading to the increase in water content. It is concluded that the deficiency of detoxification capacity caused by the lower capacity of the ascorbate-glutathione pathway and the decreased activity of protective enzymatic system might lead to the large accumulation of H2O2 and the enhancement of membrane lipid peroxidation, which might be the main cause leading to the occurrence of vitrifying shoots of P.suaveolens in tissue culture. 相似文献
2.
《林业研究》2016,(3)
The efficiency of sample-based indices proposed to quantify the spatial distribution of trees is influenced by the structure of tree stands, environmental heterogeneity and degree of aggregation. We evaluated 10 commonly used distance-based and 10 density-based indices using two structurally different stands of wild pistachio trees in the Zagros woodlands, Iran, to assess the reliability of each in revealing stand structure in woodlands. All trees were completely stem-mapped in a nearly pure(40 ha) and a mixed(45 ha) stand. First, the inhomogeneous pair correlation function [g(r)] and the Clark–Evans index(CEI) were used as references to reveal the true spatial arrangement of all trees in these stands. The sampled data were then evaluated using the 20 indices.Sampling was undertaken in a grid based on a square lattice using square plots(30 m 9 30 m) and nearest neighbor distances at the sample points. The g(r) and CEI statistics showed that the wild pistachio trees were aggregated in both stands, although the degree of aggregation was markedly higher in the pure stand. Three distance- and six density-based indices statistically verified that the wild pistachio trees were aggregated in both stands. The distance-based Hines and Hines statistic(ht) and the densitybased standardised Morisita(Ip), patchiness(IP) and Cassie(CA) indices revealed aggregation of the trees in the two structurally different stands in the Zagros woodlands and the higher clumping in the pure stand, whereas the other indices were not sensitive enough. 相似文献
3.
Yabing Zhang Chuanyan Zhou Wenqiang Lv Lihua Dai Jingang Tang Shaoqi Zhou Lihua Huang Anding Li Jianli Zhang 《林业研究》2019,(3):799-806
Carbon (C), nitrogen (N), and phosphorous (P) levels and their stoichiometry in plant components (leaves, branch trunks, roots) of trees in a karst forest and non-karst forest are compared. The results show that the C contents, C:N and C:P ratios of dominant species in the karst forest were lower than those in the non-karst forest, but the N and P and the N:P ratio were higher;C:N:P ratios in plant organs of trees in the karst forest were in the order of trunks>roots>branches>leaves. However, C:N:P ratio in the non-karst forest trees were trunks>branches>roots>leaves. Moreover, ratio of C:N:P in trunks was highest and lowest in leaves in both forests. In non-karst forest trees, N:P was in the order of leaves> roots>branches>trunks. There were no significant differences in the ratio of N:P in different plant components of trees in the karst forest. However, in karst and non-karst forest trees, the ratio of N:P in leaves was highest;positive correlations between N and P contents, and N and N:P ratios were observed in both karst and non-karst forests (p<0.001). Negative correlations between P and N:P ratios (p<0.05) were observed in karst forest trees, while positive correlations were observed in non-karst forest trees. 相似文献
4.
《林业研究》2017,(1)
CO_2concentrations in different plant communities(larch, birch, lilac, and grassland) were measured during the growing season in the Heilongjiang Forest Botanical Garden to study diurnal variation, seasonal and annual dynamics and factors that impact CO_2 concentration in different spaces. CO_2 concentration in different communities in green lands had an obvious diurnal variation,chronically decreasing, and temperature influenced the lilac area and the grassland. Seasonally, CO_2 was lowest in the larch green land(344.03 ± 23.03 μmol/mol) and highest in the grassland(360.13 ± 22.43μmol/mol). The overall trend in CO_2 concentration was autumn springsummer; temperature is the main factor controlling variation in CO_2 concentrations during the growing season; the CO_2 concentration at the larch, birch, lilac, and grassland types of sites was negatively correlated with land surface temperature and air temperature, and the CO_2 concentration at the larch and birch sites was positively correlated with atmospheric pressure. Without any obvious annual change law, further study and observation are needed. 相似文献
5.
《林业研究》2020,31(5)
The response of diversity and biomass of herbaceous functional groups along an altitudinal gradient in mountainous forests of southern Zagros, Khuzestan Province, Iran was studied by sampling vegetation in 30 circular 1000-m~2 plots in herb layer of the forest floor within 646–2447 m asl(lowland: 1000 m asl, midland:1000–2000 m asl, highland: 2000 m asl). The most important herbaceous functional groups were classified based on two aspects of growth form: annuals–perennials,grasses–forbs. Then the relationship between the diversity,richness, evenness, biomass and elevation was analyzed.The results showed that the annual functional group in the low-and midland classes, and perennial functional group in the lowland class had the highest species diversity and evenness in annual and perennial functional groups,respectively(p 0.01). The perennials in the highland class had the maximum total, above-and belowground dry biomass(p 0.01). On the other hand, the forb functional group in the lowland class had the greatest species diversity, richness, and evenness(p 0.01) and in the highland class had the maximum total dry, above-and belowgrounddry biomass in the grass and forb functional groups(p 0.01). Increasing the diversity, richness, and species evenness resulted in a decrease in the plant dry biomass. 相似文献
6.
Soil aggregation varies according to climatic and management factors, and is difficult to measure because of irregular shapes and sizes of soil aggregates. We applied fractal theory to assess soil aggregation as affected by (1) land use change from forest to savanna, (2) nutrient additions in forest, ecotone, and savanna ecosystems, and (3) tillage practice and residue treatments in an agro-ecosystem. We used fractal dimensions nonlinear (Dnon-lin) and linear (Dlin) based on number of aggregates (N) and mass of aggregates (M) (the range of values were 2.6 2.89 and 2.69 3.41, respectively) to capture the variations in the sizesof soil aggregates due to land use and treatments/management in these ecosystems. The variation in the values of nonlinear fractal dimension based on mass (DMnon-lin) was smaller in forest and savanna ecosystems with and without nutrient additions, while the variation was wider inagro-ecosystems with different management practices. Linear fractal dimensions based on number (DNlin) and mass (DMlin) of aggregates varied marginally in these ecosystems and did not capture the variations insoil aggregates well. The variations in nonlinear fractal dimension indicate that continued nitrogen loading in forest accelerates the formation of macroaggregates, whereas in savanna the situation was reversed. The values of nonlinear fractal dimensions did not show significant change after 6 years of nutrient additions in the ecotone; reflecting a buffering mechanism of this system in soil aggregate formation. On the basis of non-linear fractal dimension values, we conclude that residue retention and minimum tillage are appropriate for proper maintenance of soil aggregate stability for sustained crop production in the Indian dry land agro-ecosystems. 相似文献
7.
Panagiotis Michopoulos Kostas Kaoukis George Karetsos Theodoros Grigoratos Constantini Samara 《林业研究》2020,31(1):291-301
The fluxes of masses and the nutrients Ca,Mg,K,N,P and S were determined in the litterfall of two adjacent forest ecosystems of Hungarian oak(Quercus frainetto L.)and European beech(Fagus sylvatica L.)in a mountainous area of northeastern Greece in 2010–2015.The foliar litterfall for both species reached about 70%of the total litterfall,and was significantly higher from the other two fractions(woody and rest litterfall).The fluxes of masses and nutrients were compared between ecosystems for each fraction separately.Only one significant statistical difference was found,that of K in the woody litterfall.In addition,the stocks of masses and nutrients were calculated in the forest floors and mineral soils of the two ecosystems.Likewise,the stocks of nutrients in the forest floors and mineral soils were compared between ecosystems.In the L horizon of the forest floors,statistical differences,as a result of species effect,were found for the stocks of Ca and N.In the FH horizons,the masses and all the nutrient stocks differed significantly,as the beech plot had much higher quantities of organic matter and nutrients.These higher quantities were probably due to low soil temperatures(microclimate)and high acidity in the beech plot(species effect)that slowed down decomposition.In the mineral soils,the propagation of random error derived from random errors of the individual soil layers was an important factor in the statistical comparisons.Because of the soil acidity in the beech plot,the stocks of exchangeable base cations were significantly higher in the oak plot,whereas the other nutrient stocks did not differ. 相似文献
8.
The response of soil fauna to the litter decomposition process has received considerable attention,but this effect has not been fully examined in agroforestry systems.A 1-year in situ decomposition experiment was carried out in a pure ginkgo plantation and two ginkgo agroforestry systems using a litterbag method(11 different treatments were tested in three systems).We found that the application of different organic materials(crop residues)produced positive effects on the number of soil fauna in the ginkgo planting systems;the mixture of ginkgo leaves and corn leaves was the best performing treatment.Collembola and Acarina were the predominant groups in the litter bags and were mainly responsible for the differences among the treatments.Litter mixing promoted the abundance,richness,and diversity of soil fauna,and significant differences regarding the Shannon–Wiener index of the soil fauna were observed among the 11 treatments in July.Significantly higher soil MBC(microbial biomass carbon)and MBN(microbial biomass nitrogen)were observed in agroforestry systems than in pure ginkgo plantations.These results suggest that the practice of intercrop residue application plays an important role in enhancing soil ecosystem function in ginkgo agroforestry systems and may ultimately contribute to sustainable intercrop production,soil fertility,and local economic diversity. 相似文献
9.
Giovanni Di Matteo Luigi Perini Paolo Atzori Paolo De Angelis Tiziano Mei Giada Bertini Gianfranco Fabbio Giuseppe Scarascia Mugnozza 《林业研究》2014,25(4):839-845
We estimated water-use efficiency and potential photosynthetic assimilation of Holm oak(Quercus ilex L.) on slopes of NW and SW aspects in a replicated field test examining the effects of intensifying drought in two Mediterranean coppice forests. We used standard techniques for quantifying gas exchange and carbon isotopes in leaves and analyzed total chlorophyll, carotenoids and nitrogen in leaves collected from Mediterranean forests managed under the coppice system. We postulated that responses to drought of coppiced trees would lead to differential responses in physiological traits and that these traits could be used by foresters to adapt to predicted warming and drying in the Mediterranean area. We observed physiological responses of the coppiced trees that suggested acclimation in photosynthetic potential and water-use efficiency:(1) a significant reduction in stomatal conductance(p0.01) wasrecorded as the drought increased at the SW site;(2) foliar δ13C increased as drought increased at the SW site(p0.01);(3) variations in levels of carotenoids and foliar nitrogen, and differences in foliar morphology were recorded, and were tentatively attributed to variation in photosynthetic assimilation between sites. These findings increase knowledge of the capacity for acclimation of managed forests in the Mediterranean region of Europe. 相似文献
10.
We investigated the floristic composition of the Hyrcanian forests and the related forest-steppe ecotone in Northern Iran by using two long ecological transects, from lowland to upper mountain areas. The study was conducted during 2008 and 2009 and yielded the identification of 395 plant taxa belonging to 233 genera and 78 families. Dicots with 300 taxa were the richest groups of the flora, followed by monocots with 75 taxa, pteridophytes with 18 species,and gymnosperms with two species. The largest families were Asteraceae(33 taxa); Rosaceae(32 taxa); and Poaceae(30 taxa), and the most diverse genera included Carex(15 taxa); Alchemilla(7 taxa); and Poa, Geranium and Acer(6 taxa each). Hemicryptophytes were the most dominant life forms in the area(40 %); followed by geophytes(31.4 %); phanerophytes(15.4 %); therophytes(11.4 %); and chamaephytes(1.8 %). Phytogeographically,Euro-Siberian/Irano-Turanian elements(86 taxa, 21.8 %)and Euro-Siberian elements(85 taxa, 21.5 %) were the most common chorotypes in the area. Out of 395 taxa, 66taxa(16.7 %) were endemics and subendemics in Iran, of which 26 taxa were exclusively endemics of Iran.According to the IUCN Red List Categories, 48 threatened plant taxa were found in the study area. Plant diversity, life form, and chorotypes in the current study were compared with similar transect studies in other areas of the Hyrcanian forests and in different altitudinal belts, using S?renson similarity indices. Floristic composition of the surveyed transects demonstrated almost 50 % similarity between them. 相似文献
11.
Green stem cuttings, obtained from 2-year old managed stockplants, with 30, 50 or 100% leaf area were rooted in a non-mist propagator following treatment with 0, 0.2, 0.4 or 0.8% IBA solution. The rooted cuttings were transferred to polythene pots and grown in a nursery for 12 weeks. Rooting percentages of IBA treated cuttings did not significantly differ from those of control cuttings. Likewise leaf area did not significantly influence the percentage of cuttings that rooted. The effects of leaf area and/or IBA treatment in the propagation unit were manifested on the growth of rooted cuttings in polythene pots. Number of roots increased with the increase in IBA concentration and this response was enhanced by increasing leaf area. Root biomass increased with the increase in leaf area even in the control cuttings. Root development was highly influenced by the leaf area and IBA, and hence the growth of rooted cuttings. Cuttings treated with 0.2 or 0.4% IBA at 100% leaf area yielded the best performing rooted cuttings. 相似文献
12.
The present study aimed at examining the amenability of Stereospermum suaveolens DC. to vegetative propagation through rooting of leafy stem cuttings using low-cost polythene propagators. Rooting efficiencies
of cuttings derived from two selected matured mother trees were studied using four different concentrations of Indole-3-butyric
acid (IBA), viz. 0, 0.1, 0.2 and 0.4%. IBA applied at 40 μg per cutting (0.4% concentration) resulted in significantly higher
rooting responses compared to the control (P < 0.05). Differences in the length of longest root for all the treatments were significant (P = 0.05) with cuttings treated with 0.2% IBA producing the longest root. The highest survival after 3 weeks of transference
of the rooted cuttings into polythene bags was found for rooted cuttings treated with 0.2% IBA. Effects of three fertilizer
treatments viz. T0 (no fertilizer), T1 (10 g of Urea + 20 g of TSP + 10 g of MOP dissolved in 1 l of water) and T2 (10 g of Urea + 20 g of TSP + 10 g of MOP dissolved in 2 l of water) on initial growth of established rooted cuttings (stecklings)
of Stereospermum suaveolens in polythene bags were also measured for a period of 90 days. Stem length, collar diameter and leaf area of the stecklings
increased throughout the observation period. Increments of both stem length and collar diameter were significantly higher
for the stecklings treated with T2 in comparison with the control while increment of leaf area was found the highest for the stecklings treated with T1. The study clearly indicated that Stereospermum suaveolens is amenable to vegetative propagation technique using juvenile single-node leafy cuttings and the initial growth performance
of the established rooted cuttings (stecklings) in polythene bags under different fertilizer treatments was satisfactory. 相似文献
13.
Techniques for the vegetative propagation of Ulmus villosa, an indigenous agroforestry tree species of the north-western sub-Himalayas, were developed. Studies investigated the effects on rooting of stem cuttings of growth regulators with sucrose and fungicide (0%, 0.2% p-HBA + 5% sucrose + 5% captan, and IBA concentrations ranging from 0.2 to 1.0% plus each containing, 0.2% p-HBA + 5% sucrose + 5% captan). Cuttings were collected at two times of the year (February and July) from two types of stock plant (seedling and mature trees) and from two positions within the shoots (lower and upper nodes). Cuttings collected in February were leafless, while those collected in July had about 80 cm2 leaf areas. These cuttings were then set under a mist unit. Percentage rooting and primary root number differed significantly between treated and untreated cuttings. Treatment of cuttings with chemical formulations of 0.4% IBA + 0.2% p-HBA + 5% sucrose + 5% captan maximized rooting (82.0%), while, survival (80%) and primary root number (14.7) were greatest with: (i) 0.6% IBA + 0.2% p-HBA + 5% sucrose + 5% captain and (ii) 0.8% IBA + 0.2% p-HBA + 5% sucrose + 5% captan, respectively. Rooting success and root number were better in propagules set in February rather than in July. Cuttings from seedlings rooted better, with more roots ensuring better survival, than cuttings from mature trees (12–13-years-old). In cuttings from seedlings, those cuttings collected from the lower part of the shoots had greater percentage rooting and primary root number than those from the upper part. Maximum root regeneration (100%), roots number (19.0), and survival (98.5%) were achieved when the time of collection (February) and shoot position were optimized. Rooting was best when cuttings had higher levels of sugar, total carbohydrate and peroxidase enzyme activity, and low N. 相似文献
14.
A study to determine the best vegetative propagation protocol was carried out for Dalbergia melanoxylon. Natural regeneration of D. melanoxylon is limited due to poor seed viability and germination. The effects of age of donor plant, indole-3-butyric acid (IBA) treatment
and cutting position on rooting of stem cuttings were investigated. The effect of age of donor plant was significant (P < 0.001) only for percent rooting, position effect was significant for all studied parameters, and IBA treatment at 300 ppm
was insignificant only for percent callusing. Interactive effects of cutting position × age of donor and cutting position × IBA
treatment were significant for percent rooting and root number per cuttings while interactive effect of age × IBA was significant
only for percent rooting. Observed difference in rooting performance could be due to uneven distribution of root promoting
and inhibiting substances with respect to age of donor plant and IBA which intensified root formation. Cuttings from juvenile
donor plant performed better in all rooting parameters than mature donor plant. The highest percentages in rooting were (71.11%)
and (24.42%) for cuttings from juvenile and mature donor plants, respectively. IBA treated cuttings produced higher percentage
rooting, number of roots and root length than untreated cuttings which revealed a strong IBA influence on rooting ability
of stem cuttings in D. melanoxylon. It was concluded that stem cuttings should be taken from juvenile donor plants and basal cutting position should be used
to produce high quality planting stock material for the afforestation and conservation programme of D. melanoxylon. 相似文献
15.
Tarit Kumar Baul Mohammad Mosharraf Hossain Mohammad Mezbahuddin Mohammed Mohiuddin 《林业研究》2011,22(3):409-416
In this study we investigated the rooting ability and the growth performance of juvenile single-node leafy stem cuttings of Litsea monopetala (Roxb) Pers. collected from two mature mother trees preserved in the hill forest of Chittagong district, Bangladesh. The rooting ability of cuttings was studied under 0%, 0.1%, 0.2% and 0.4% indole-3-butyric acid (IBA) treatments. Significantly better rooting response (p ≤ 0.05) was observed with 0.1% IBA compared to control (0% IBA). The mean number of roots and the length of the longest root of cuttings in different treatments showed no significant difference (p≤0.05). After transfer into polythene bags from non-mist propagator, rooted cuttings treated with 0%, 0.1% and 0.2% IBA demonstrated the highest (100 ± 0.00%) survival capacity. The mean number of shoots developed in cuttings in the polythene bags in first three weeks varied significantly (p≤0.05) among the treatments. Effects of three fertilizer treatments, viz. T0 (no fertilizer), T1 (10g Urea, 20g TSP, 10g MOP dissolved in 1 L water) and T2 (10g Urea, 20g TSP, 10g MOP dissolved in 2 L water) on initial growth ofstecklings were also measured over a 90-days period. The increment of leaf area of stecklings was significantly higher (p≤0.05) under T0 compared with that under T1 and T2 while the increment of stem length, collar diameter and root biomass varied insignificantly among different fertilizer treatments. The results suggest that rooting juvenile single-node leafy stem cuttings could be an effective mean of regenerating L. monopetala. The application of 0.1% IBA concentration is recommended for rooting of juvenile leafy stem cuttings and application of fertilizer appeared unnecessary for the subsequent growth of stecklings in polythene bags. 相似文献
16.
Cuttage and air layering were evaluated as means of vegetative propagation of the tropical woody tree, Inga feuillei. Effects of moisture management systems, leafiness, auxin application, and stem diameter on rooting of semihardwood cuttings were investigated. Cuttings were taken from 2-year-old seed-propagated, greenhouse-grown stock plants. Compound leaves were either reduced in area or removed entirely before auxin pretreatment with 0, 0.3, or 0.8% indolebutyric acid (IBA) followed by sticking under mist or in a polyethylene enclosure. Leafless cuttings did not root regardless of moisture management system or auxin pretreatment, whereas 55% rooting of leafy cuttings was observed. Leafy cuttings rooted significantly better under mist than in a polyethylene enclosure. Auxin treatment at the higher level increased rooting percentage approximately two fold for larger diameter cuttings (8.1 to 20 mm), but had no effect on the smaller cuttings (2 to 8 mm), and resulted in an approximately three fold increase in the number of roots/rooted cutting for both stem diameter classes. Auxin treatment did not affect rooting percentage of leafy softwood cuttings under fog, however it did increase the number of roots per rooted cutting. One hundred percent of air layered shoots rooted within 5 weeks with or without auxin pretreatment, and all rooted layers survived transplanting to soil. Possible implications of this research on agroforestry, selection, genetic improvement, and conservation are discussed. 相似文献
17.
E. F. de Andres J. Alegre J. L. Tenorio M. Manzanares F. J. Sanchez L. Ayerbe 《Agroforestry Systems》1999,46(2):113-121
The vegetative propagation of the multipurpose leguminous shrub Colutea arborescens from leafless hardwood cuttings was examined.
Specific attention was paid to the factors: 1) cutting origin (apical, medial or basal); 2) indole-3-butyric acid (IBA) treatment
(0, 50 or 200 ppm); and 3) relative humidity of the propagation environment (high or low humidity). After 120 days, 67.6%
of the C. arborescens cuttings had rooted. The rooting ability of the cuttings was significantly influenced by the cutting
origin and hormonal treatment. No effects on rooting percentage were shown by the relative humidity of the propagation environment.
The best rooting response (95%) was shown by basal cuttings treated with 200 ppm IBA. Cutting origin, IBA treatment and relative
humidity were significantly related to the root number, longest root length and the fresh/dry biomass of roots and leaves.
Under conditions of high humidity, basal cuttings treated with 200 ppm IBA gave rise to the most highly developed plants.
The positive relationship between sprouting and rooting suggests that successful propagation is related to the shoot formation
potential of the cutting's food reserves. These findings were used to propose an efficient method for the vegetative propagation
of C. arborescens plants.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
18.
Catherine Ky-Dembele Jules Bayala Antoine Kalinganire Fatoumata Tata Traoré Bréhima Koné Alain Olivier 《Southern Forests》2016,78(3):193-199
Pterocarpus santalinoides is a multipurpose tree species, native to Africa and South America. However, excessive pruning for fodder is jeopardising its seed production leading to poor regeneration in the Sahel region. Efficient vegetative propagation techniques may overcome this concern. To address this issue, four experiments were conducted to determine the effects of substrate (Experiment 1), cutting type (Experiments 2 and 3), genotype (Experiment 4) and auxins (Experiments 1, 2, and 4) on rooting ability in a non-mist propagating unit in Mali. Among the four tested substrates, no significant differences were found in rooting parameters, including the percentage of rooted cuttings, the number of roots and secondary roots per rooted cutting, and the length of the longest root per rooted cutting. Semi-hardwood cuttings rooted significantly better than softwood cuttings with a higher percentage of rooted cuttings (31.7?±?6.5% vs. 9.2?±?2.7%), greater number of roots (8.2?±?4.8 vs 1.5?±?0.7), greater number of secondary roots (16.7?±?4.2 vs 4.6?±?1.8) and longer root length (17.8?±?12.0?cm vs 2.3?±?1.0?cm) per rooted cutting. In addition, one-node cuttings cut below the second and third nodes, respectively, had the highest rooting success (88% and 90%, respectively) followed by cuttings cut below the fourth node (68%) and the first node (62%). Significant differences in the percentage of rooted cuttings were found among 12 randomly selected genotypes with rooting varying from 2% to 75%.The results also showed that auxin application, especially IBA, had a significant effect on rooting success, the number of roots and secondary roots per rooted cutting. Single-node cuttings, and cuttings of 10?cm length with multiple softwood and semi-hardwood nodes, can be used with or without auxin treatment for successful clonal propagation of P. santalinoides. The results of the present study indicated that P. santalinoides is amenable to clonal propagation using low-cost propagators and sand-based rooting media. 相似文献
19.
This study investigates the optimal conditions for the vegetative propagation of the multipurpose leguminous shrub Colutea istria from leafy stem cuttings. The conditions examined included 1) the origin of the cutting (apical, medial or basal), 2) indole-3-butyric
acid (IBA) treatment (0, 50, 200 or 400 mg/l), 3) the relative humidity of the propagation environment (high or low), 4) wounding
base of cutting, and 5) the season in which the cutting was taken. The rooting ability of the cuttings was significantly influenced
by all these factors. Winter cuttings obtained from the middle and base of the branch showed a greater rooting capacity than
those harvested from the apical area, while autumn cuttings harvested from the middle and apical areas of the branch showed
a greater rooting capacity than those harvested from the base. Winter cuttings showed the best rooting capacity than autumn
cuttings. IBA treatment (200 mg/l), high humidity (98-100%), and wounding increased the rooting capacity of the cuttings. The sprout
of new leaves was higher in basal cuttings, while IBA and wounding also significantly enhanced the root number of rooted cuttings.
A method for vegetatively propagating C. istria plants is given. 相似文献
20.