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1.
猪传染性胃肠炎(TGE)是由猪传染性胃肠炎病毒(TGEV)引起猪只的一种高度接触性胃肠道传染病,临床症状主要表现为腹泻、呕吐和脱水,该病为影响养猪业健康发展的重要疫病之一,世界动物卫生组织将其列为法定报告的B类动物疫病。近年来,国内外许多研究者在对猪传染性胃肠炎的诊断与检测方法上进行了大量的研究,并建立了相关的实验室诊断检测技术,这些检测技术主要包括病理诊断、病毒的分离鉴定、电镜检测、血清学诊断和分子生物学诊断方法等。本文对猪传染性胃肠炎主要的诊断技术进行了综述,以期为该病在临床的快速诊断及防控提供参考。 相似文献
2.
猪传染性胃肠炎是一种高度接触性、急性肠道传染病。脱水、呕吐和腹泻是猪传染性胃肠炎的主要临床症状表现,为了使该病得到有效的防治,必须做好尽早的诊断,以保证能够及时得到治疗。本研究从猪传染性胃肠炎的病原着手,具体分析了猪传染性胃肠炎的诊断和治疗方法。 相似文献
3.
猪传染性胃肠炎是由猪传染性胃肠炎病毒引起的一种急性、病毒性传染病,该病严重影响着猪的生产性能,给养猪业造成极大的经济损失。基于此,笔者结合工作实践体会,阐述了猪传染性胃肠炎的诊断与防治措施,以期为养猪场诊治该病提供技术参考。 相似文献
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猪传染性胃肠炎病毒和猪流行性腹泻病毒二联RT-PCR检测方法的建立 总被引：1，自引：0，他引：1
为了研究出一种快速、敏感、特异的诊断猪传染性胃肠炎和猪流行腹泻的方法,试验参照国内外已发表的猪传染性胃肠炎病毒(TGEV)和猪流行性腹泻病毒(PEDV)基因序列及其相关的RT-PCR检测方法,根据猪传染性胃肠炎病毒S蛋白基因和猪流行性腹泻病毒s蛋白基因各设计1套特异性通用引物,扩增目的带分别为426 bp和584 bp.结果表明:建立的猪传染性胃肠炎病毒和猪流行性腹泻病毒二联RT-PCR检测方法具有快速、敏感、特异等优点,可为猪传染性胃肠炎病毒和猪流行性腹泻病毒的检测、流行病学调查及疫苗使用等奠定基础. 相似文献
6.
猪传染性胃肠炎病毒属冠状病毒科冠状病毒属成员,主要引起以仔猪呕吐、腹泻、脱水和死亡为特征的传染性胃肠炎。随着集约化养猪业的发展,该病造成的损失越来越大。近年来,猪传染性胃肠炎病毒各方面的研究都取得了一定进展。本文就国内外猪传染性胃肠炎病毒基因组结构、蛋白质功能、致病机理研究进展进行综述,并介绍了分子生物学技术在诊断方法和新型疫苗开发上的应用情况。 相似文献
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根据GenBank上猪传染性胃肠炎病毒S基因的保守序列,设计1对引物,建立了检测猪群中的猪传染性胃肠炎病毒的RT-PCR方法.试验结果表明,该RT-PCR方法敏感、特异,适于临床样品检测,为猪传染性胃肠炎病毒的临床诊断和流行病学调查奠定了基础. 相似文献
8.
为探讨猪传染性胃肠炎病的防治措施,本文从传染性胃肠炎病毒的病原、流行病学特点、临诊症状、病理变化和诊断逐一进行阐述,最后提出治疗方法和防治措施。目的是提高对猪传染病胃肠炎病的认识,加强对该病的防范。 相似文献
9.
间接竞争ELISA检测TGEV方法的建立 总被引：1，自引：0，他引：1
猪传染性胃肠炎(transmissible gastroenteri-tis,TGE)是由冠状病毒科猪传染性胃肠炎病毒(transmissible gastroenteritis virus,TGEV)引起的以仔猪呕吐、腹泻、严重脱水为特征的消化道传染病。病毒粒子随粪便大量排出,是造成病毒扩大传播、仔猪大批死亡的重要原因。采取粪便样品进行病原检测,对该病早期诊断具有重要意义。目前针对TGEV感染有多种快速诊断方法,概括起来可分为针对病毒结构蛋白进行的各种免疫学诊断技术和近些年发展的以病毒核酸为基础的分子生物学检测方法。其中免疫学方法以其特异性强、易操作、不需要昂贵仪器等特点… 相似文献
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猪传染性胃肠炎病毒检测的新方法 总被引：1，自引：0，他引：1
猪传染性胃肠炎是由猪传染性胃肠炎病毒（TGEV）引起的以猪呕吐、腹泻、脱水为特征的传染病,对新生仔猪具有高度致死率（通常100％）的传染病,给养猪业带来严重危害,已成为重要的猪病毒性腹泻病之一。因此对该病的诊断显得尤为重要。检测TGEV的传统方法主要有病毒分离、中和试验、免疫荧光抗体试验、酶联免疫吸附试验和电镜技术等,这些方法对于检测TGEV和防制TGE起到了重要作用。目前cDNA探针、RT—PCR已成为国际上检测TGEV的主要方法,为临床上诊断TGE提供灵敏、快速的方法。 相似文献
11.
Immunoperoxidase intibody (IPA) test was developed for detecting antibody against transmissible gastroenteritis (TGE) virus of pigs. The IPA antibody titers in sera collected in the field from 82 pigs were approximately seven times higher than those obtained in a serum-neutralization test. The correlation between the TGE antibody concentrations in the IPA and serum neutralization tests was positive (r = +0.74). The IPA tests appears to have the potential for routine laboratory use for serologic diagnosis of TGE. 相似文献
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K W Theil L J Saif E H Bohl A G Agnes E M Kohler 《American journal of veterinary research》1979,40(5):719-721
A 3-day-old suckling pig with diarrhea was necropsied, and immunofluorescent microscopic examination of the small intestinal mucosa, together with immune electron microscopic examination of the large intestinal contents, provided a presumptive diagnosis of a concurrent infection with transmissible gastroenteritis (TGE) virus and porcine rotavirus. Immunofluorescent microscopic, immune electron microscopic, and serologic data obtained from gnotobiotic pigs experimentally inoculated with the large intestinal contents of the suckling pig confirmed this diagnosis. Two gnotobiotic pigs, convalescent from previous TGE viral infections, became infected with porcine rotavirus only. However, another gnotobiotic pig, convalescent from a previous porcine rotaviral infection, became infected with TGE virus only, following inoculation with the large intestinal contents of the suckling pig. 相似文献
13.
猪传染性胃肠炎和猪流行性腹泻诊断方法研究进展 总被引：3，自引：0，他引：3
猪传染性胃肠炎和猪流行性腹泻均是由冠状病毒引起的以仔猪发生呕吐、腹泻和脱水为特征的两种传染病。目前 ,对这两种传染病的诊断方法主要有病毒中和试验 ,免疫荧光法 ,免疫电镜法 ,EL ISA法和 RT-PCR法。随着分子生物学的不断发展 ,EL ISA法和 RT-PCR法以其敏感性高、特异性强越来越受到人们的重视。文章就各种诊断方法近年来的研究进展进行了综述 ,并简明地指出了研究中存在的问题以及未来展望 ,可为这两种传染病诊断方法的进一步研究提供一些新的思路 相似文献
14.
Solid phase immune electron microscopy for diagnosis of transmissible gastroenteritis in pigs 总被引：3，自引：0，他引：3
A serological trapping technique is described for detecting transmissible gastroenteritis (TGE) virus in faeces. The technique involves the coating of electron microscope grids with protein A and specific TGE virus antiserum. Optimal conditions for performing this solid phase immune electron microscopy technique were a concentration 250 micrograms ml-1 of protein A; 1:100 diluted rabbit anti-TGE virus hyperimmune serum for coating the grids and overnight incubation with virus samples. The possibility of detecting coronavirus in crude faeces was highly improved with solid phase immune electron microscopy, compared with conventional negative staining electron microscopy, by specific trapping of virus and prevention of adsorption of contaminants. The sensitivity of the method was evaluated by coded investigation of a dilution series of stock virus mixed with different pools of faeces. The improvement of virus detection in faeces by solid phase immune electron microscopy, compared with standard electron microscopy was at least 100-fold. Faecal shedding of coronavirus by pigs infected with virulent and attenuated strains of TGE virus was studied. Virus detection in faeces by a standard electron microscopy technique was not practical, since the virus was obscured by a large quantity of debris in the faeces. By using solid phase immune electron microscopy, however, the aspect of the specimens on the grids improved so much that, in addition to more common immunofluorescence, the technique might be useful as a diagnostic test for TGE. Virus was detected in daily faecal samples from one or two days after experimental infection with virulent TGE virus, until death in five out of eight animals. Unlike immunofluorescence, solid phase immune electron microscopy may be used for diagnosis in living animals. 相似文献
15.
Cross-protection studies between the feline infectious peritonitis (FIP) and the porcine transmissible gastroenteritis (TGE) viruses were conducted in cats, pigs and pregnant gilts. Cats vaccinated with TGE virus developed neutralizing antibodies against TGE virus and low titer antibody against FIP virus detected by an indirect fluorescent antibody technique but were not protected against a virulent FIP virus challenge. Baby pigs and pregnant gilts vaccinated with FIP virus did not develop detectable antibodies to TGE virus. Nevertheless, it appeared that vaccination of swine with FIP virus conferred some immunity against TGE virus infection. Seventeen-day-old pigs vaccinated with two doses of FIP virus had a 67% survival rate following a virulent TGE virus challenge, and 75% of the 3-day-old pigs suckling either FIP or TGE-virus-vaccinated gilts survived virulent TGE virus infection in contrast to 0% survival of baby pigs suckling unvaccinated gilts. 相似文献
16.
Cell-mediated immune responses of suckling pigs inoculated with attenuated or virulent transmissible gastroenteritis virus 总被引：1，自引：0，他引：1
Two litters of suckling pigs seronegative for transmissible gastroenteritis (TGE) virus were orally inoculated with live attenuated (P115) or virulent (M5C) strains of TGE virus. A third seronegative litter (controls) was given cell culture fluids from uninfected cells. Lymphocytes were collected from blood, spleen, mesenteric lymph nodes, and Peyer patches of euthanatized pigs at 0 day and approximately weekly until 26 days after exposure and at approximately 45 days after exposure. Sera were tested for virus-neutralizing antibody titers by use of plaque reduction. Lymphocytes were tested in a lymphocyte proliferation assay for uptake of [3H]thymidine after incubation with the homologous or the heterologous strain of inactivated TGE virus or uninfected cell culture fluids. Only pigs inoculated with virulent TGE virus developed clinical signs of TGE and shed virus. However, all pigs inoculated with TGE virus seroconverted at 6 days after exposure. Responses of lymphocytes from all sources from TGE virus-inoculated pigs peaked between 6 and 14 days after exposure. Pigs inoculated with virulent TGE virus had higher lymphocyte proliferative responses and neutralizing antibody titers than did pigs inoculated with attenuated TGE virus. Cessation of virus shedding coincided with the peak of lymphocyte proliferative responses. The highest responses were with intestinal lymphocytes (mesenteric lymph nodes and Peyer patches) from pigs inoculated with virulent TGE virus. The responses of intestinal lymphocytes from pigs inoculated with attenuated virus were not significantly different from those of pigs inoculated with cell culture fluid. Lymphocytes collected from all sources, except blood from M5C-inoculated pigs, had significantly (P less than 0.05) higher responses to the homologous than to the heterologous TGE virus stimulation.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
17.
某大型猪场临床仔猪见不明原因腹泻,发病率高,而对妊娠母猪普遍接种ＴＧＥ和ＰＥＤ疫苗后收到了明显的防病效果。 相似文献
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J P Siegel L L Hungerford W F Hall 《Journal of the American Veterinary Medical Association》1991,199(11):1579-1583
Commercial production data base records from 2 Illinois farms, on which epizootic or enzootic transmissible gastroenteritis (TGE) was experienced, were accessed for an epidemiologic study. Risk factors investigated were sow parity, source of sows, location of farrowing crates, and breeding practices. At farm 1, an epizootic was experienced; at farm 2, an epizootic of TGE followed by enzootic TGE was experienced. Initially, crude risk ratios were calculated for these risk factors, and the crude risk ratios were subsequently adjusted for confounders and interactions, using multiple logistic regression techniques. After adjustment, parity-3 sows were 2.3 times more likely to have litters with TGE than were sows of all other parities on farm 1, and parity-1 sows were 2.6 times more likely to have litters that experienced TGE than were sows of all other parities on farm 2. A single boar on each farm was linked to increased likelihood of a sow's litter contracting epizootic TGE on each farm. Enzootic TGE was maintained by the periodic influx of outside-source gilts on farm 2; these gilts were 2.2 times more likely to have litters with TGE than were sows derived from farm 2. Sows housed in farrowing crates located under the cold air inlet of farm 2 were 1.7 times as likely as sows located in other rows to have litters with enzootic TGE. 相似文献
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L J Kemeny 《American journal of veterinary research》1978,39(4):703-705
A virologic survey was conducted to determine the frequency of transmissible gastroenteritis (TGE) virus infection in farm-raised sows. Pharyngeal swab specimens collected in an abattoir were examined for TGE virus by inoculation onto swine-testes cell culures. The virus was detected in 61 (3%) of a sample of 2,058 Iowa sows after slaughter. All TGE viral isolates, given orally to 2- or 3-day-old pigs, caused acute gastroenteritis and in some cases death. All pigs that recovered from illness had serum antibody to TGE virus. 相似文献
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M Asagi T Ogawa T Minetoma K Sato Y Inaba 《American journal of veterinary research》1986,47(10):2161-2164
A reversed passive hemagglutination (RPHA) method was developed for the detection of transmissible gastroenteritis (TGE) virus in the fecal specimens from pigs. Ovine erythrocytes fixed with glutaraldehyde and treated with tannic acid were coated with anti-TGE virus swine antibodies, which were purified by affinity chromatographic technique linked with purified TGE virus. The RPHA test was done by the Microtiter method. Erythrocytes coated with purified specific antibodies were agglutinated by TGE virus, but not by porcine rotavirus or porcine enterovirus. The reaction was specifically inhibited by antiserum against TGE virus, confirming the specificity of the reaction. A litter of seven 3-day-old pigs was orally inoculated with TGE virus, and fecal specimens were obtained once a day and serum was obtained every 4th day. With the RPHA test, TGE virus was detected in the diarrheal feces; all of the inoculated pigs developed virus-neutralization antibody for the TGE virus. The RPHA test detected TGE virus in feces from pigs with naturally occurring diarrhea. The RPHA test detected TGE virus in 5 of 6 fecal specimens (80%), whereas the positive rate was only 50% (3/6) for the immunofluorescent staining of primary cultures of porcine kidney cells inoculated with the specimens. The advantages of the RPHA method are simplicity, high sensitivity, and rapid to do. 相似文献