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1.
Muscle growth was studied in larvae of sea bass, Dicentrarchus labrax L., reared at two temperatures: real ambient temperature ( congruent with 15 degrees C during vitelline phase and increased gradually) and 19 degrees C from fertilization until the end of larval development. Muscle cellularity, body length and body weight were measured. Early temperature influenced larval development and so, pre-larval phase finished earlier at 19 degrees C than at ambient temperature (4 and 6 days, respectively). Temperature also affected muscle growth such that at hatching and at mouth opening hypertrophy of muscle fibres was greater at 19 degrees C (P < 0.05), whereas hyperplasia was similar in both groups. After 25 days, the cross-sectional area of the white muscle was greater at 19 degrees C (P < 0.05), which was mainly associated with a higher proliferation of new white muscle fibres. At this stage the body length was also higher at 19 degrees C. Metamorphosis finished earlier in fish reared at 19 degrees C (52 days) than at natural temperature (82 days). At this developmental stage body length and cross-sectional area of the myotome were similar in both groups. However, muscle cellularity differed between groups. Thus, hypertrophy of muscle fibres was higher in fish reared at ambient temperature (P < 0.05), whereas proliferation of new muscle fibres was higher at 19 degrees C (P > 0.05).  相似文献   

2.
The objective of this study was to determine the relationship between body weight and the size, number and proportion of muscle fibre types in the pig semitendinosus muscle at birth. Based on weight at birth, 68 specimens selected from 21 different litters from the same farm were allotted in two equal groups (G1 and G2). G1 included 34 piglets ≤1.1 kg and G2 34 pigs ≥2 kg. Fifteen piglets per group were killed at birth and the remaining 19 piglets in each group were reared until weaning (21 days) and post-weaning (67 days). The weight and total cross-sectional area of the semitendinosus muscle were recorded at birth. Then, type I and type II fibres from the superficial portion of the muscle were identified according to histochemistry and immunohistochemistry techniques and percentages, average size of each fibre type, and the total number of muscle fibres were estimated by morphometry. Birth weight in G1 was 54.74% lower than that in G2. Correspondingly, the total cross-sectional area of the semitendinosus, as well as the size and number of muscle fibres, was significantly lower in G1 ( P  <   0.001). Weight at birth still influenced weights at weaning and post-weaning, hence it was 43.17% and 28.38% lower respectively in G1. It is concluded that pig weight at birth is associated with muscle cellularity of the semitendinosus muscle of pig, which may influence the postnatal muscle growth and final size of muscle fibres and meat quality.  相似文献   

3.
In order to determine the temperature effect on the axial muscle growth of sea bass, a stock of larvae was subjected to the following incubation and cultivation temperatures, respectively: 15 degrees C/ambient, 15/17 degrees C, 17 degrees C/ambient and 17/17 degrees C. In all groups the cross-sectional area of white and red muscles and the number and average area of the white and red muscle fibres were quantified. Results showed that the embryonic period, pre-larval phase and the end of metamorphosis were accelerated at higher temperatures. During the endogenous feeding period, muscle growth took place by fibrillar hypertrophy, and was not influenced by the temperature. Thereafter (external feeding) muscular hyperplasia began, and growth of all the muscular parameters was favoured by the effect of high incubation and cultivation temperatures, with the latter having higher influence. High incubation temperature had an slight effect on muscle growth and body length, which was only observed from 15 days. Metamorphosis finished at 3 +/- 0.4 cm in all the larvae, but this length was earlier reached at higher temperatures. At 120 days, the largest growth was obtained in the larvae maintained at a higher temperature.  相似文献   

4.
Fibre-type differentiation of lateral musculature has been studied in gilthead sea bream Sparus aurata (L.) and sea bass Dicentrarchus labrax (L.) during post-larval development using ultrastructural, histochemical and morphometric techniques. The study showed three muscle layers: red, intermediate (or pink) and white. Initially, most of the red muscle showed low myosin ATPase (m-ATPase) activity fibres, whereas near the transverse septum some small high m-ATPase activity fibres appeared and later acquired a rosette aspect. Afterwards, during adult growth the red muscle showed a histochemical mosaic appearance. The pink muscle in sea bass was observed at the beginning of juvenile development by the oxidative technique (NADH-RT reaction) whereas in gilthead sea bream it was also observed at the end of larval development. The pink layer consists of high m-ATPase activity fibres. However, along the muscle development other low and moderate m-ATPase activity fibres were observed close to the red and white muscles, respectively. The white muscle of juvenile fish showed a histochemical mosaic appearance near the pink muscle. In adult specimens the mosaic white muscle spread out occupying the whole of the myotome. Morphometric analysis shows a significant increase in mean fibre diameter during post-larval development, as shown by the Student's t-test (hypertrophic growth). Skewness and kurtosis values of fibre diameters point to the generation of a new fibres from the myosatellite cells (hyperplastic growth).  相似文献   

5.
The histochemical profiles - mATPase and NADH-TR reactions - of the red and white muscle fibres of gilthead sea bream and sea bass were determined from the first week after hatching. Modifications of the mATPase technique by combinations of pH/time/molarity were carried out in order to compare the sensitivity of the myosin ATPase of each muscle fibre type of the lateral muscle. Results showed that the staining of muscle fibres was independent of small modifications in the technique. The intermediate ‘pink’ muscle was histochemically defined towards the end of the larval life and is considered to be implicated in the growth of the myotome. A layer of external cells was observed, by electron microscopical examination, between the connective tissue of the skin and the superficial red muscle fibres of larvae and postlarvae. It is suggested that the external cells are unlikely to be a source of red muscle fibres and implicated on the growth of the myotome, but rather a part of the dermatome. The timing, areas and mechanisms of hyperplastic growth of the myotome were defined and discussed.  相似文献   

6.
Pig breeding is aimed at improving lean meat production ability as well as meat quality, and muscle fibre characteristics may be important for enhancing these traits. Therefore, new molecular markers have been demanded for selecting lean meat production ability and meat quality in live animals. Myogenin belongs to the MyoD gene family, and is a candidate gene responsible for muscle fibre characteristics. We identified a new single nucleotide polymorphism (SNP) site in the 5' upstream region of the myogenin gene (nucleotides C and T). A total of 252 pigs of three breeds were genotyped by polymerase chain reaction–restriction fragment length polymorphism using BspCNI . Additionally, they were genotyped for the previously detected Msp I site in the 3'-flanking region (alleles A and B). The CCBB diplotype had the highest frequency over breeds, followed by TCBB and CCAB. The other diplotypes were not found in studied pigs. Association analysis performed for the markers found that the TCBB diplotype has desirable effects on the total number of fibres (p   < 0.002), fibre cross-sectional area (p   < 0.06), and loin eye area (p   < 0.001) than the other diplotypes. Moreover, the diplotype had the highest muscle pH value (p   < 0.07) and all meat quality traits were near the upper limit of the normal range as a reddish pink, firm and non-exudative (RFN) pork. Therefore, we suggest that selection for the myogenin diplotypes could improve total muscle fibre number, size and lean meat production ability with good meat quality.  相似文献   

7.
Reasons for performing study: The trotting speed of Norwegian‐Swedish Coldblooded Trotters (NSCTs) is, although inferior to Standardbred speeds, gradually increasing. This development is mainly attributed to breeding progresses. Characteristics of the middle gluteal muscle have recently been described in a small number of these horses but the actual spread in muscle parameters within the population is unknown. Objectives: To describe the characteristics of the middle gluteal muscle and their naturally occurring variability in young NSCT horses and investigate possible association with body size, individual breeding index and early training. Methods: Biopsies of the middle gluteal muscle of 144 NSCT horses born in 2005 were collected at age 17–24 months. The horses were randomly selected by stratified sampling with gender and paternal progeny as stratification factors. The muscle samples were analysed for fibre type composition, fibre type areas, capillary supply and the activity of the key metabolic enzymes citrate synthase, 3‐OH‐acyl‐CoA‐dehydrogenase and lactate dehydrogenase. Correlation analysis including muscle parameters and body size, individual breeding index and early training was performed. Results: Large interindividual differences were identified in muscle characteristics. Male horses had higher percentages of type IIA fibres and lower percentages of type IIX fibres than age‐matched females. Fibre type areas followed the order type IIX>IIAX>IIA>I, while the capillary supply per fibre type area increased in the opposite order. No gender‐related differences were found in fibre type areas or capillary supply. Height was correlated to the relative area of type I fibres. Early training was associated with increased oxidative capacity and increased percentage of type IIA fibres. The lowest breeding index was found in untrained horses. Conclusions: Muscle characteristics differ among young NSCT horses, but the innate oxidative capacity seems to be lower than in Standardbreds of comparable age. Early training may influence on these parameters.  相似文献   

8.
The extensor digitorum longus (EDL) and soleus (SOL) muscle fibres from albino rats submitted to experimental chronic alcoholism were evaluated in accordance with their metabolic and morphometric profiles. Twenty-seven male animals aged 4 months and weighing approximately 400 g were used. The animals were divided into three groups: control, isocaloric and alcoholic and sacrifices were carried out after 5, 10 and 15 months. The muscles were dissected, removed, cross-sectioned in a cryostat and submitted to the NADH (nicotinamide adenine dinucleotide) reaction. The SO (slow-twitch-oxidative), FG (fast-twitch-glycolytic) and FOG (fast-twitch-oxidative-glycolytic) muscle fibre types exhibited a polygonal, triangular or rounded shape and did not present noteworthy modifications in either muscles during the study. The cross-sectional areas of the fibres from the studied muscles did not present significant differences during the observations. Fibre area behaved similarly in the alcoholic animals up to the 10th month, i.e. it was decreased, as also observed in the other groups. At 15 months, however, all fibres were increased, with a predominance of FG fibres in the SOL muscle. Changes in fibre population were observed mainly in the SOL muscle of alcoholic animals: SO fibres were initially increased in number but decreased after the 10th month, and the opposite was observed for the population of FG fibres. FOG fibres increased linearly in number throughout the experiment. The statistical analysis showed nevertheless that the fibre population and cross-sectional area changes were not significant. In the alcoholic animals quantitative variations of muscle fibres were more evident in the SOL muscle, suggesting that the SOL muscle is more sensitive to the toxic action of ethanol. The results concerning the increased fibre diameter in alcoholic animals would be associated with muscle oedema induced directly or indirectly by the ethanol.  相似文献   

9.
The body length and diameter of muscle fibres from the right hypaxial tail myomeres were measured in 100 sea bass larvae (Dicentrarchus labrax, L.) at ages of 40 to 90 days, from a fish farm in Croatia. The larvae were kept at a temperature of 19-20 degrees C and fed with standard feed for their respective ages. Body lengths of larvae 40-90 days old varied from 8 to 42 mm, increasing significantly in 10-day intervals (p < 0.001), except between days 50 and 60 when there was no increase in the body length. The measured diameters of muscle fibres ranged from 10 to 80 microm, the mean value increasing except between days 50 and 60. The fibre diameter in larvae aged up to 60 days was 10-40 microm, while that in larvae older than 70 days was 51-80 microm. In 90-day-old larvae there were no fibres of 10-20 microm in diameter; however, the number of fibers of 61-80 microm diameter increased. A statistically significant concordance was determined between the body length and fibre diameter of larvae 70 to 90 days old. Between days 50 and 60, arrested growth of the larval body and corresponding decrease in fibre diameter was observed. From day 45 until days 59, larvae were fed with Artemia nauplii and microparticles of formulated diets. From the day 60, minced fish was added to formulated diets. This change in diet probably resulted in the observed growth arrest.  相似文献   

10.
本试验旨在研究饲料中添加肌肉生长抑制素抑制肽对海鲈生长性能、体组成、血清生化指标及肝脏与血清免疫指标的影响。以初始均重为(9.05±0.05)g的海鲈幼鱼为试验动物,暂养1周后,挑选规格一致的健康试验鱼480尾,随机分为4组,每组4个重复(水族箱),每个重复放养30尾鱼。4组试验鱼分别投喂肌肉生长抑制素抑制肽添加水平为0(对照)、0.25%、0.50%、0.75%的试验饲料135 d。结果显示:1)0.50%组的增重率(WGR)、特定生长率(SGR)和成活率(SR)均显著高于对照组(P0.05),各添加组的饲料系数(FCR)均显著低于对照组(P0.05),且以0.50%组的FCR最低;2)各添加组的背肌粗脂肪含量均显著低于对照组(P0.05),0.50%组的背肌粗蛋白质含量显著高于对照组(P0.05),全鱼的水分、粗蛋白质、粗脂肪、粗灰分和背肌的水分、粗灰分含量在各组之间没有显著差异(P0.05);3)0.25%组的血清总蛋白(TP)、白蛋白(ALB)和总胆固醇(TC)含量显著低于其他各组(P0.05),血清甘油三酯(TG)含量各组间不存在显著差异(P0.05);4)0.50%和0.75%组的血清碱性磷酸酶(ALP)活性显著高于对照组(P0.05),0.25%和0.50%组的肝脏溶菌酶(LZM)活性显著高于对照组(P0.05),肝脏与血清的总抗氧化能力(T-AOC)和肝脏超氧化物歧化酶(SOD)活性各组间不存在显著差异(P0.05)。综上所述,在饲料中添加肌肉生长抑制素抑制肽能够促进海鲈的生长并提高免疫能力,本试验条件下最适添加水平为0.50%。  相似文献   

11.
研究旨在通过测定豁眼鹅、皖西白鹅、浙东白鹅肌肉纤维直径、横切面积和肌纤维的密度,探讨不同鹅品种的肌纤维特性差异.结果表明,豁眼鹅胸肌肌纤维直径、横切面积显著大于浙东白鹅、皖西白鹅(P< 0.05),而肌纤维密度表现为相反的趋势.豁眼鹅的腿肌肌纤维直径和横切面积显著大于皖西白鹅(P< 0.05),而豁眼鹅的腿肌肌纤维密度显著小于皖西白鹅(P< 0.05).品种内不同性别之间部分指标也存在显著差异,主要表现在皖西白鹅和浙东白鹅的肌纤维横切面积和直径上.相关性分析表明,肌纤维的直径与肌纤维横切面积呈极显著正相关(P< 0.01),肌纤维直径和横切面积与肌纤维密度呈极显著负相关(P< 0.01).由此可知,不同品种间肌纤维特性存在差异,同品种间不同性别间的肌纤维特性也存在差异.  相似文献   

12.
To estimate the direct and correlated responses in meatiness and meat quality, simulated selection was applied using one‐trait selection and index selection including muscle fibre traits. In a total of 2024 pigs of German Landrace, Large White, Leicoma, and Schwerfurter breeds, carcass composition, meat quality, and fibre characteristics of the M. longissimus lumborum were analysed and genetic parameters were calculated by using REML variance component estimation. Coefficients of heritability ranged from low to moderate (growth traits: h2 = 0.22–0.32; meat quality traits: h2 = 0.17–0.27; muscle fibre traits: h2 = 0.12–0.20). The total fibre number and the frequency of white fibres correlated positively with live weight (rg = 0.44 and 0.44, respectively) and with loin muscle area (rg = 0.38 and 0.19, respectively) while the relationships to pH value and drip loss were not as close (rg = ?0.29–0.19). Selection indices were constructed from live weight and muscle area, from pH value and drip loss, and from muscle structure traits. As expected, simulated selection for live weight or loin muscle area lead to direct positive effects but these were associated with negative effects on meat quality traits (selection intensities: SI = 0.1; 0.5). Using selection for an index from loin area and muscle structure (loin muscle area + total fibre number – frequency of white fibres – frequency of giant fibres) the adverse effect on meat quality was minimized (responses in pH value: ?0.05; ?0.01) or was changed towards desired direction (responses in drip loss: ?0.65%; ?0.26%). The results show that fibre characteristics of pig muscle can be used as selection criteria for simultaneous improvement of carcass composition and meat quality in pigs by including indices developed from performance and fibre traits.  相似文献   

13.
Fish have the ability to compensate for set-backs in growth as a result of fasting. When food levels are restored, growth in these fish can increase over and above normal rates. This phenomenon, known as “compensatory growth”, has been studied with respect to enhancing food conversion efficiency. However, the mechanisms by which food intake activates an increase in somatic growth, and especially in muscle growth, are not well understood. In this study, we report first on the isolation of two complete cDNAs sequences encoding sea bass (Dicentrarchus labrax) myostatin and fibroblast growth factor 6 (FGF6), which have been shown to be major genetic determinants of skeletal muscle growth. The open reading frames of myostatin (376 amino acids) and FGF6 (209 amino acids) showed 97–63% and 87–62% sequence identity with other vertebrate myostatins and FGF6s, respectively. We also report on the expression profile of myostatin and FGF6 in sea bass skeletal muscle in response to different feeding regimens, as quantified by real-time RT-PCR. Nutritional status significantly influenced the myostatin expression levels in muscle, inducing an up-regulation during fasting and a down-regulation during the recovery from fasting, whereas the muscular FGF6 mRNA levels were not significantly affected by the feeding status of the animals. These findings suggest that myostatin has an inhibitory role in muscle growth in response to different feeding regimens, whereas FGF6 is not involved in the muscle compensatory growth induced by refeeding.  相似文献   

14.
Abstract

This study was conducted to investigate the effects of garlic and ginger oils on hematological and biochemical health characteristics of sea bass Dicentrarchus labrax. Fish were exposed to garlic oil (0.01 or 0.02 mL/L), ginger oil (0.01 or 0.02 mL/L), or a combination of the two oils (each oil at a concentration of 0.005 or 0.01 mL/L) for 96 h via bath immersion. Results showed that the red blood cell count, hematocrit (%), hemoglobin (Hb) concentration (g/dL), mean corpuscular volume (μm3), mean corpuscular Hb (pg), and mean corpuscular Hb concentration (%) were not significantly affected by herb oil exposure. However, some changes in biochemical variables were observed. Sea bass exposed to the 0.005-mL/L garlic oil–ginger oil mixture exhibited a significant increase in serum glucose. Serum total protein and albumin levels decreased in sea bass that were exposed to a garlic oil–ginger oil mixture (0.005 or 0.01 mL/L) or to garlic oil at 0.02 mL/L. Serum globulin levels decreased and triglyceride levels increased in sea bass exposed to 0.02-mL/L garlic oil or to the 0.01-mL/L mixture. The serum lipase level decreased and the cholesterol level increased in fish that were exposed to 0.02-mL/L garlic oil. In summary, ginger oil at 0.01–0.02 mL/L can be used without negative effects, while the garlic oil or garlic oil–ginger oil mixture should be applied at a concentration below 0.005 mL/L for bath immersion of sea bass. This is the first study to examine how garlic oil and ginger oil exposure via bath immersion affects the hematological and biochemical status of sea bass.

Received March 19, 2012; accepted July 2, 2012  相似文献   

15.
A bovine tail was cross-sectioned at four vertebrae, to expose its muscles - dorsal extensors, ventral flexors and the lateral flexors that pass between the transverse processes. Muscle cross-sectional areas were measured at these faces and muscle sections were stained to distinguish slow and fast fibre types. From the muscle and fibre area data and fibre type occurrences, preliminary conclusions are drawn about the role of different muscles. The tail as a model for muscle studies is briefly discussed.  相似文献   

16.
The middle gluteal muscle of five, two-year-old untrained trotters was investigated by repeated needle biopsy sampling over a training period of six months. A second group of five, three-year-old untrained horses was included to examine the effect of growth. After the training period increases were found in the relative distribution of slow twitch (ST) fibres from 18 per cent to 25 per cent and fast twitch (FTa) fibres from 36 per cent to 45 per cent, and a decrease in FTb fibres from 46 per cent to 30 per cent. A proportionally equal reduction (approximately 18 per cent) in the cross sectional area of all fibre types was observed after the first two months of training succeeded by an increase to approximately pretraining levels at the end of the period. The number of capillaries per fibre was enhanced from 1.7 to 2.4. Proliferation of capillaries occurred around fibres of all types. The metabolic adaptations showed increases in the activities of 3-hydroxy-acyl-CoA dehydrogenase (HAD) (50 per cent) and citrate synthase (31 per cent). Growth had no effect on the relative fibre type distribution nor the capillary per fibre ratio, but as the mean fibre area increased 36 per cent (primarily because of increases in FT fibres) the number of capillaries/mm2 was lower in the older untrained horses (350 capillaries/mm2) compared with the younger untrained ones (460 capillaries/mm2). Increase with growth was found in the activity of phosphorylase and HAD and a decrease was seen in the activity of hexokinase. It is concluded that the training programme exclusively induced alterations which improved the aerobic capacity of the muscle.  相似文献   

17.
The transverse red and white muscle area, the superficial red muscle fibres area and their percentages of mitochondria (%mit), myofibrils (%myof) and sarcoplasm (%sarc) were determined in the Mediterranean teleost gilthead sea bream, Sparus aurata (L.). Fish aged from hatching to 78 days were studied. The proportional growth of the red and white muscles was higher for the red muscle in the first half of the larval stage (1-35 days). Then the opposite relationship was observed. The hypertrophic growth of the superficial muscle fibres was continuous except in the first week after hatching. The percentage of mitochondria and percentage of myofibrils showed a significant change just after the half of the larval stage. Whereas the %mit of the superficial muscle fibres was higher than %myof from the first week after hatching to 35 days (average 66.64%), then the %mit decreased significantly and at 73-78 days both parameters were close to an average value of 50%. The meaning of these morphological changes is discussed in relation to the functional role of the red muscle of larvae and the onset of the gills respiration.  相似文献   

18.
We evaluated the influence of dietary gelatinized starch and protein on the fatty acid composition of muscle in rainbow trout and European sea bass and on the susceptibility of flesh to lipid peroxidation. The possibility that flesh peroxidation could be accounted for by lipogenesis and the deposition of fat was also explored. The inclusion of gelatinized starch in the diet of rainbow trout improved growth with respect to that observed in fish fed crude starch (P<.001). This was especially noticeable at the lowest concentration of dietary protein tested (P = .037); suggesting that gelatinized starch may partially replace protein in the production of energy without inducing a negative effect on growth. However, in European sea bass, the gelatinization of starch and dietary protein concentration showed no significant effect on final body weight. The intramuscular neutral lipid concentration of the sea bass was reduced by the gelatinization of dietary starch (P = .034). The highest dietary protein concentration increased the proportion of saturated fatty acids in the neutral (P = .0742) and polar (P = .0033) lipid fractions. The dietary inclusion of high levels of protein in rainbow trout led to a lower concentration of total (n-3) (P = .0457) and (n-6) (P = .0522) fatty acids and a higher concentration of total monounsaturated fatty acids (P = .0006). The inclusion of gelatinized starch led to a lower concentration of (n-3) fatty acids (P = .0034) and a higher concentration of saturated fatty acids (P = .0007). The polar fraction was hardly affected by the same treatment. A significantly lower susceptibility of the dorsal muscle to oxidation was observed in groups of European sea bass fed gelatinized starch (P<.01). A similar trend was observed in rainbow trout, although differences were not significant. The findings suggest that the digestible protein concentration of nutrient-dense diets for rainbow trout and European sea bass can be reduced with a beneficial effect on tissue lipid oxidation and no negative effects on growth and muscle composition.  相似文献   

19.
This study uses non-invasive evoked surface electromyography (SEMG) to investigate postnatal muscle development in pigs, and to assess any correlation between recorded signal parameters and muscle fibre types in two different skeletal muscles. Four litters (n = 43) of Large White × Landrace pigs were used. Evoked SEMG measurements were taken on days 2, 5, 14, 26, 60 and 151 post partum from m. Longissimus dorsi (LD) and on days 14, 26, 60 and 151 post partum from m. Biceps femoris (BF). A third of each litter was slaughtered at days 27, 61 and 153 post partum. Biopsy samples for LD and BF were taken to categorize muscle fibre types. For LD there was a significant increase in compound muscle action potential (CMAP) parameters from day 2 to day 5 post partum, whilst for BF significant increases occurred from days 14 to 26 post partum. Fibre type development in both muscles showed a significant decrease in type IIA fibre number (P < 0.001) and an increase in type IIB fibre number (P < 0.001). Analysis of CMAP parameters in relation to fibre type percentages showed significant positive correlations between percentage of type I fibres in BF and CMAP Area (r = 0.71; P = 0.05), SlopeL (r = 0.79; P = 0.02) and Corr Peak (r = 0.78; P = 0.02) and a negative correlation with SlopeT (r = 0.89; P = 0.003) at day 151. A greater CMAP Area and Corr Peak as well as steeper leading and trailing slopes were on the other hand correlated to fewer type IIB fibres.

It is concluded that 1) changes in the evoked CMAP are muscle-specific during early postnatal development in the pig, and 2) in spite of a small sample size, the correlations between CMAP signal parameters and fibre type percentages warrant further investigation.  相似文献   


20.
We compared the histochemical activity of succinate dehydrogenase (SDH) and the intensity of reaction to the presence of myoglobin (Mb) in the same muscle fibres m. longissimus thoracis (MLT), m. semimembranosus (MSM), m. rectus femoris (MRF) and caput longum m. tricipitis brachii (MTB). At the same time we also ascertained the myoglobin content of one gram of fresh muscle. We found that the muscle fibre in all the muscles studied in which there is the most intensive reaction in the test for SDH are also the most intensively coloured in the test for Mb. There is also the same relationship in intermediary and white fibres. The reaction in testing for SDH on a cross section is greatest in the subsarcolemmic area. Similar tendencies appear in the distribution of Mb, although they are less marked and only in fibres with intensive reaction. Different muscles have different myoglobin levels. Muscles with a higher proportion of red and intermediary fibres contain more myoglobin than muscles with a high proportion of white fibres. The quantitative values of the levels of Mb (g/fresh muscle) are not, however, proportional to the share of individual types of muscle fibres in the muscles studied.  相似文献   

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